pycom111g02100

Belongs to the universal ribosomal protein uL15 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0002131
Physical Location & Seq
Forward (+)
1261231 .. 1261680
450 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g02100.1

Sequence Viewer

Length: 450 bp
ATGACGACGAGGTTCAAGAAGAACCGCAAGAAGCGGGGCCACGTCAGTGCCGGTCACGGGCGTATCGGCAAGCACCGCAAGCATCCCGGCGGTCGCGGTAACGCCGGAGGAATGCACCACCACCGGATCCTCTTCGACAAGTACCATCCAGGGTATTTCGGTAAGGTCGGTATGAGGTACTTCCACAGGCTGCGCAACAAGTTCCATTGCCCAATCGTCAACCTCGACAAGCTGTGGTCGCTTGTACCCCAGGACGTGAAGGACAAGGCCGCCGCCGCCAAGGGAGGAAGCGAGGCGCCGTTGCTCGACGTCACTCAGTTCGGCTACTTCAAGGTGTTGGGGAAGGGCGTTTTGCCGTCTCAGCCGTTGGTAGTGAAGGCCAAGCTGATCTCAAAGACCGCCGAGAAGAAGATCAAGGAGGCCGGTGGCGCCGTCGTGCTCACCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L27A

Protein Analysis

150

Amino Acids

16.48

Weight (kDa)

10.67

Isoelectric Point (pI)

21.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27A PF00828 72 - 146 4e-15 Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 312
Acc16I TGCGCA 1 cut(s) 194
AccB1I GGYRCC 2 cut(s) 295, 428
AccII CGCG 1 cut(s) 96
AclWI GGATC 2 cut(s) 121, 134
AcyI GRCGYC 3 cut(s) 296, 309, 429
AfaI GTAC 3 cut(s) 143, 179, 246
AfiI CCNNNNNNNGG 1 cut(s) 57
AgsI TTSAA 2 cut(s) 16, 331
AjiI CACGTC 2 cut(s) 43, 256
AjnI CCWGG 2 cut(s) 148, 249
AleI CACNNNNGTG 1 cut(s) 45
AluBI AGCT 2 cut(s) 232, 385
AluI AGCT 2 cut(s) 232, 385
Alw21I GWGCWC 1 cut(s) 441
Alw26I GTCTC 1 cut(s) 363
AlwI GGATC 2 cut(s) 121, 134
AoxI GGCC 4 cut(s) 37, 267, 378, 420
ApeKI GCWGC 1 cut(s) 190
AspLEI GCGC 3 cut(s) 195, 298, 431
AspS9I GGNCC 1 cut(s) 37
AsuC2I CCSGG 1 cut(s) 87
AsuHPI GGTGA 1 cut(s) 433
BaeI ACNNNNGTAYC 2 cut(s) 46, 79
BamHI GGATCC 1 cut(s) 126
BanI GGYRCC 2 cut(s) 295, 428
Bbv12I GWGCWC 1 cut(s) 441
BbvI GCAGC 1 cut(s) 177
BccI CCATC 1 cut(s) 153
BceAI ACGGC 4 cut(s) 283, 340, 349, 416
BciT130I CCWGG 2 cut(s) 150, 251
BcnI CCSGG 1 cut(s) 87
BcoDI GTCTC 1 cut(s) 363
BfoI RGCGCY 2 cut(s) 299, 432
BisI GCNGC 4 cut(s) 191, 270, 273, 276
BlsI GCNGC 4 cut(s) 192, 271, 274, 277
Bme1390I CCNGG 3 cut(s) 87, 150, 251
BmgBI CACGTC 2 cut(s) 43, 256
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 4 cut(s) 38, 128, 297, 430
BmrFI CCNGG 3 cut(s) 87, 150, 251
BmsI GCATC 1 cut(s) 91
BpuMI CCSGG 1 cut(s) 87
BsaHI GRCGYC 3 cut(s) 296, 309, 429
BsaJI CCNNGG 3 cut(s) 149, 249, 279
BsaWI WCCGGW 1 cut(s) 123
Bsc4I CCNNNNNNNGG 1 cut(s) 57
Bse118I RCCGGY 2 cut(s) 50, 422
Bse3DI GCAATG 1 cut(s) 205
BseBI CCWGG 2 cut(s) 150, 251
BseDI CCNNGG 3 cut(s) 149, 249, 279
BseGI GGATG 2 cut(s) 82, 145
BseLI CCNNNNNNNGG 1 cut(s) 57
BseMI GCAATG 1 cut(s) 205
BseMII CTCAG 2 cut(s) 329, 374
BseXI GCAGC 1 cut(s) 177
Bsh1236I CGCG 1 cut(s) 96
Bsh1285I CGRYCG 1 cut(s) 94
BshFI GGCC 4 cut(s) 39, 269, 380, 422
BshNI GGYRCC 2 cut(s) 295, 428
BsiEI CGRYCG 1 cut(s) 94
BsiHKAI GWGCWC 1 cut(s) 441
BsiSI CCGG 5 cut(s) 51, 87, 105, 124, 423
BslI CCNNNNNNNGG 1 cut(s) 57
BsmAI GTCTC 1 cut(s) 363
BsmBI CGTCTC 1 cut(s) 363
BsmI GAATGC 1 cut(s) 117
BsnI GGCC 4 cut(s) 39, 269, 380, 422
Bsp1286I GDGCHC 1 cut(s) 441
Bsp143I GATC 3 cut(s) 126, 387, 411
BspANI GGCC 4 cut(s) 39, 269, 380, 422
BspCNI CTCAG 2 cut(s) 328, 373
BspFNI CGCG 1 cut(s) 96
BspLI GGNNCC 4 cut(s) 38, 128, 297, 430
BspPI GGATC 2 cut(s) 121, 134
BspT107I GGYRCC 2 cut(s) 295, 428
BsrDI GCAATG 1 cut(s) 205
BsrFI RCCGGY 2 cut(s) 50, 422
BssAI RCCGGY 2 cut(s) 50, 422
BssECI CCNNGG 3 cut(s) 149, 249, 279
BssMI GATC 3 cut(s) 126, 387, 411
BssNI GRCGYC 3 cut(s) 296, 309, 429
BssT1I CCWWGG 1 cut(s) 279
Bst2UI CCWGG 2 cut(s) 150, 251
Bst6I CTCTTC 1 cut(s) 137
BstACI GRCGYC 3 cut(s) 296, 309, 429
BstC8I GCNNGC 2 cut(s) 71, 80
BstDEI CTNAG 3 cut(s) 315, 360, 447
BstF5I GGATG 2 cut(s) 82, 145
BstFNI CGCG 1 cut(s) 96
BstH2I RGCGCY 2 cut(s) 299, 432
BstHHI GCGC 3 cut(s) 195, 298, 431
BstKTI GATC 3 cut(s) 129, 390, 414
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 3 cut(s) 126, 387, 411
BstMCI CGRYCG 1 cut(s) 94
BstMWI GCNNNNNNNGC 6 cut(s) 75, 79, 238, 275, 361, 428
BstNI CCWGG 2 cut(s) 150, 251
BstSCI CCNGG 3 cut(s) 85, 148, 249
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 1 cut(s) 177
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
BsuRI GGCC 4 cut(s) 39, 269, 380, 422
BtrI CACGTC 2 cut(s) 43, 256
BtsCI GGATG 2 cut(s) 82, 145
BtsIMutI CAGTG 1 cut(s) 52
Cac8I GCNNGC 2 cut(s) 71, 80
CfoI GCGC 3 cut(s) 195, 298, 431
Cfr10I RCCGGY 2 cut(s) 50, 422
Cfr13I GGNCC 1 cut(s) 37
Csp6I GTAC 3 cut(s) 142, 178, 245
CviJI RGCY 9 cut(s) 39, 190, 232, 269, 324, 364, 380, 385, 422
CviKI_1 RGCY 9 cut(s) 39, 190, 232, 269, 324, 364, 380, 385, 422
CviQI GTAC 3 cut(s) 142, 178, 245
DdeI CTNAG 3 cut(s) 315, 360, 447
DinI GGCGCC 2 cut(s) 297, 430
DpnI GATC 3 cut(s) 128, 389, 413
DpnII GATC 3 cut(s) 126, 387, 411
Eam1104I CTCTTC 1 cut(s) 137
EarI CTCTTC 1 cut(s) 137
Eco130I CCWWGG 1 cut(s) 279
EcoRII CCWGG 2 cut(s) 148, 249
EcoT14I CCWWGG 1 cut(s) 279
EgeI GGCGCC 2 cut(s) 297, 430
EheI GGCGCC 2 cut(s) 297, 430
ErhI CCWWGG 1 cut(s) 279
Esp3I CGTCTC 1 cut(s) 363
FaiI YATR 1 cut(s) 173
FauI CCCGC 1 cut(s) 27
Fnu4HI GCNGC 4 cut(s) 191, 270, 273, 276
FokI GGATG 2 cut(s) 69, 132
Fsp4HI GCNGC 4 cut(s) 191, 270, 273, 276
FspI TGCGCA 1 cut(s) 194
GlaI GCGC 3 cut(s) 194, 297, 430
GluI GCNGC 4 cut(s) 191, 270, 273, 276
HaeII RGCGCY 2 cut(s) 299, 432
HaeIII GGCC 4 cut(s) 39, 269, 380, 422
HapII CCGG 5 cut(s) 51, 87, 105, 124, 423
HhaI GCGC 3 cut(s) 195, 298, 431
Hin1I GRCGYC 3 cut(s) 296, 309, 429
Hin6I GCGC 3 cut(s) 193, 296, 429
HinP1I GCGC 3 cut(s) 193, 296, 429
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HpaII CCGG 5 cut(s) 51, 87, 105, 124, 423
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 1 cut(s) 220
Hpy188III TCNNGA 1 cut(s) 16
Hpy8I GTNNAC 1 cut(s) 220
Hpy99I CGWCG 3 cut(s) 10, 311, 437
HpyAV CCTTC 3 cut(s) 253, 337, 370
HpyCH4IV ACGT 3 cut(s) 42, 255, 309
HpyCH4V TGCA 1 cut(s) 115
HpyF10VI GCNNNNNNNGC 6 cut(s) 75, 79, 238, 275, 361, 428
HpyF3I CTNAG 3 cut(s) 315, 360, 447
HpySE526I ACGT 3 cut(s) 42, 255, 309
Hsp92I GRCGYC 3 cut(s) 296, 309, 429
HspAI GCGC 3 cut(s) 193, 296, 429
KasI GGCGCC 2 cut(s) 295, 428
Kzo9I GATC 3 cut(s) 126, 387, 411
Lsp1109I GCAGC 1 cut(s) 177
LweI GCATC 1 cut(s) 91
MaeII ACGT 3 cut(s) 42, 255, 309
MaeIII GTNAC 3 cut(s) 53, 98, 310
MalI GATC 3 cut(s) 128, 389, 413
MboI GATC 3 cut(s) 126, 387, 411
MboII GAAGA 4 cut(s) 31, 124, 418, 421
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 1 cut(s) 441
Mly113I GGCGCC 2 cut(s) 296, 429
MnlI CCTC 8 cut(s) 3, 101, 140, 168, 233, 278, 286, 412
MslI CAYNNNNRTG 1 cut(s) 45
MspI CCGG 5 cut(s) 51, 87, 105, 124, 423
MspR9I CCNGG 3 cut(s) 87, 150, 251
Mva1269I GAATGC 1 cut(s) 117
MvaI CCWGG 2 cut(s) 150, 251
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 6 cut(s) 75, 79, 238, 275, 361, 428
NarI GGCGCC 2 cut(s) 296, 429
NciI CCSGG 1 cut(s) 87
NdeII GATC 3 cut(s) 126, 387, 411
NlaIV GGNNCC 4 cut(s) 38, 128, 297, 430
NmeAIII GCCGAG 1 cut(s) 427
NmuCI GTSAC 2 cut(s) 53, 310
NsbI TGCGCA 1 cut(s) 194
OliI CACNNNNGTG 1 cut(s) 45
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PctI GAATGC 1 cut(s) 117
PkrI GCNGC 4 cut(s) 192, 271, 274, 277
PluTI GGCGCC 2 cut(s) 299, 432
Psp6I CCWGG 2 cut(s) 148, 249
PspGI CCWGG 2 cut(s) 148, 249
PspN4I GGNNCC 4 cut(s) 38, 128, 297, 430
PspPI GGNCC 1 cut(s) 37
PsuI RGATCY 1 cut(s) 126
RsaI GTAC 3 cut(s) 143, 179, 246
RsaNI GTAC 3 cut(s) 142, 178, 245
RseI CAYNNNNRTG 1 cut(s) 45
SatI GCNGC 4 cut(s) 191, 270, 273, 276
Sau3AI GATC 3 cut(s) 126, 387, 411
Sau96I GGNCC 1 cut(s) 37
ScrFI CCNGG 3 cut(s) 87, 150, 251
SduI GDGCHC 1 cut(s) 441
SfaNI GCATC 1 cut(s) 91
SfoI GGCGCC 2 cut(s) 297, 430
SmiMI CAYNNNNRTG 1 cut(s) 45
SspDI GGCGCC 2 cut(s) 295, 428
StyD4I CCNGG 3 cut(s) 85, 148, 249
StyI CCWWGG 1 cut(s) 279
TaiI ACGT 3 cut(s) 45, 258, 312
TaqI TCGA 3 cut(s) 135, 225, 306
TauI GCSGC 3 cut(s) 272, 275, 278
TscAI CASTG 1 cut(s) 52
TseFI GTSAC 2 cut(s) 53, 310
TseI GCWGC 1 cut(s) 190
Tsp45I GTSAC 2 cut(s) 53, 310
TspRI CASTG 1 cut(s) 52
ZraI GACGTC 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.