RchiOBHm_Chr5g0018391

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002131
Physical Location & Seq
Forward (+)
12949484 .. 12950328
845 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29860

Sequence Viewer

Length: 441 bp
ATGACGACCCGGTTGAAGAAAAACCGAAAGAAGAGAGGCCACGTCAGCGCCGGTCACGGCCGGATCGGGAAGCACCGGAAGCACCCCGGCGGGCGGGGAAACGCCGGAGGGATGCACCACCACCGGATCCTGTTCGACAAGTACCACCCGGGGTATTTCGGTAAAGTGGGCATGAGGTACTTCCACAAGCTCCGCAACAAGTTCTACTGCCCCATCGTCAACGTCGACAAGCTCTGGTCCCTGGTGCCGCAGGAGGTCAAGGACAAGGCCACCAAAAACGACGTGCCGTTGATTGACGTGACGCAGCACGGGTTCTTCAAGATTTTGGGTAAGGGGAAGCTGCCGGCGGACCGGCCTGTGGTGGTGAAGGCCAAGCTCATTTCGAAGGTGGCCGAGAAGAAGATCAAGGAGGCGGGCGGCGCTGTGGTTCTCACCGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L27A

Protein Analysis

146

Amino Acids

16.39

Weight (kDa)

10.61

Isoelectric Point (pI)

21.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27A PF00828 72 - 143 5.5e-16 Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 244
AccI GTMKAC 1 cut(s) 225
AciI CCGC 8 cut(s) 90, 94, 193, 248, 347, 413, 417, 435
AclWI GGATC 3 cut(s) 71, 121, 134
AcoI YGGCCR 2 cut(s) 58, 390
AfaI GTAC 2 cut(s) 143, 179
AfiI CCNNNNNNNGG 2 cut(s) 93, 358
AgsI TTSAA 2 cut(s) 16, 319
AjiI CACGTC 3 cut(s) 43, 283, 298
AjnI CCWGG 1 cut(s) 240
AluBI AGCT 4 cut(s) 190, 232, 340, 376
AluI AGCT 4 cut(s) 190, 232, 340, 376
AlwI GGATC 3 cut(s) 71, 121, 134
Ama87I CYCGRG 1 cut(s) 148
AoxI GGCC 6 cut(s) 37, 58, 267, 353, 369, 390
ApeKI GCWGC 2 cut(s) 304, 340
AspLEI GCGC 2 cut(s) 50, 422
AspS9I GGNCC 2 cut(s) 237, 349
AsuC2I CCSGG 4 cut(s) 10, 87, 149, 150
AsuHPI GGTGA 2 cut(s) 376, 424
AsuII TTCGAA 1 cut(s) 383
AvaI CYCGRG 1 cut(s) 148
AvaII GGWCC 2 cut(s) 237, 349
BamHI GGATCC 1 cut(s) 126
BanI GGYRCC 1 cut(s) 244
BbvI GCAGC 2 cut(s) 316, 327
BccI CCATC 1 cut(s) 221
BceAI ACGGC 2 cut(s) 73, 271
BciT130I CCWGG 1 cut(s) 242
BcnI CCSGG 4 cut(s) 10, 87, 149, 150
BfaI CTAG 1 cut(s) 439
BfoI RGCGCY 2 cut(s) 51, 423
BisI GCNGC 4 cut(s) 248, 305, 341, 418
BlsI GCNGC 4 cut(s) 249, 306, 342, 419
Bme1390I CCNGG 5 cut(s) 10, 87, 149, 150, 242
Bme18I GGWCC 2 cut(s) 237, 349
BmeT110I CYCGRG 1 cut(s) 148
BmgBI CACGTC 3 cut(s) 43, 283, 298
BmgT120I GGNCC 2 cut(s) 237, 349
BmiI GGNNCC 3 cut(s) 128, 239, 246
BmrFI CCNGG 5 cut(s) 10, 87, 149, 150, 242
BmsI GCATC 1 cut(s) 102
Bpu14I TTCGAA 1 cut(s) 383
BpuMI CCSGG 4 cut(s) 10, 87, 149, 150
BsaJI CCNNGG 4 cut(s) 85, 148, 149, 240
BsaWI WCCGGW 2 cut(s) 75, 123
Bsc4I CCNNNNNNNGG 2 cut(s) 93, 358
Bse118I RCCGGY 3 cut(s) 50, 343, 351
BseBI CCWGG 1 cut(s) 242
BseDI CCNNGG 4 cut(s) 85, 148, 149, 240
BseGI GGATG 1 cut(s) 117
BseLI CCNNNNNNNGG 2 cut(s) 93, 358
BseX3I CGGCCG 1 cut(s) 58
BseXI GCAGC 2 cut(s) 316, 327
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 6 cut(s) 39, 60, 269, 355, 371, 392
BshNI GGYRCC 1 cut(s) 244
BsiEI CGRYCG 1 cut(s) 61
BsiHKCI CYCGRG 1 cut(s) 148
BslFI GGGAC 1 cut(s) 223
BslI CCNNNNNNNGG 2 cut(s) 93, 358
BsmFI GGGAC 1 cut(s) 223
BsnI GGCC 6 cut(s) 39, 60, 269, 355, 371, 392
BsoBI CYCGRG 1 cut(s) 148
Bsp119I TTCGAA 1 cut(s) 383
Bsp143I GATC 3 cut(s) 63, 126, 402
BspACI CCGC 8 cut(s) 90, 94, 193, 248, 347, 413, 417, 435
BspANI GGCC 6 cut(s) 39, 60, 269, 355, 371, 392
BspLI GGNNCC 3 cut(s) 128, 239, 246
BspPI GGATC 3 cut(s) 71, 121, 134
BspT104I TTCGAA 1 cut(s) 383
BspT107I GGYRCC 1 cut(s) 244
BsrFI RCCGGY 3 cut(s) 50, 343, 351
BssAI RCCGGY 3 cut(s) 50, 343, 351
BssECI CCNNGG 4 cut(s) 85, 148, 149, 240
BssMI GATC 3 cut(s) 63, 126, 402
Bst2UI CCWGG 1 cut(s) 242
Bst6I CTCTTC 1 cut(s) 26
BstBI TTCGAA 1 cut(s) 383
BstC8I GCNNGC 3 cut(s) 92, 345, 415
BstF5I GGATG 1 cut(s) 117
BstH2I RGCGCY 2 cut(s) 51, 423
BstHHI GCGC 2 cut(s) 50, 422
BstKTI GATC 3 cut(s) 66, 129, 405
BstMBI GATC 3 cut(s) 63, 126, 402
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 3 cut(s) 45, 79, 419
BstNI CCWGG 1 cut(s) 242
BstSCI CCNGG 5 cut(s) 8, 85, 147, 148, 240
BstV1I GCAGC 2 cut(s) 316, 327
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
BstZI CGGCCG 1 cut(s) 58
BsuRI GGCC 6 cut(s) 39, 60, 269, 355, 371, 392
BtrI CACGTC 3 cut(s) 43, 283, 298
BtsCI GGATG 1 cut(s) 117
Cac8I GCNNGC 3 cut(s) 92, 345, 415
CfoI GCGC 2 cut(s) 50, 422
Cfr10I RCCGGY 3 cut(s) 50, 343, 351
Cfr13I GGNCC 2 cut(s) 237, 349
Cfr9I CCCGGG 1 cut(s) 148
CpoI CGGWCCG 1 cut(s) 349
CseI GACGC 1 cut(s) 310
Csp6I GTAC 2 cut(s) 142, 178
CspI CGGWCCG 1 cut(s) 349
CviAII CATG 1 cut(s) 172
CviQI GTAC 2 cut(s) 142, 178
DpnI GATC 3 cut(s) 65, 128, 404
DpnII GATC 3 cut(s) 63, 126, 402
EaeI YGGCCR 2 cut(s) 58, 390
EagI CGGCCG 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
EciI GGCGGA 1 cut(s) 362
EclXI CGGCCG 1 cut(s) 58
Eco47I GGWCC 2 cut(s) 237, 349
Eco52I CGGCCG 1 cut(s) 58
Eco88I CYCGRG 1 cut(s) 148
EcoRII CCWGG 1 cut(s) 240
FaeI CATG 1 cut(s) 175
FaiI YATR 1 cut(s) 173
FaqI GGGAC 1 cut(s) 223
FatI CATG 1 cut(s) 171
FauI CCCGC 3 cut(s) 83, 87, 406
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 4 cut(s) 248, 305, 341, 418
FokI GGATG 1 cut(s) 124
Fsp4HI GCNGC 4 cut(s) 248, 305, 341, 418
FspBI CTAG 1 cut(s) 439
GlaI GCGC 2 cut(s) 49, 421
GluI GCNGC 4 cut(s) 248, 305, 341, 418
HaeII RGCGCY 2 cut(s) 51, 423
HaeIII GGCC 6 cut(s) 39, 60, 269, 355, 371, 392
HgaI GACGC 1 cut(s) 310
HhaI GCGC 2 cut(s) 50, 422
Hin1II CATG 1 cut(s) 175
Hin6I GCGC 2 cut(s) 48, 420
HinP1I GCGC 2 cut(s) 48, 420
HincII GTYRAC 2 cut(s) 220, 226
HindII GTYRAC 2 cut(s) 220, 226
HphI GGTGA 2 cut(s) 376, 424
Hpy166II GTNNAC 2 cut(s) 220, 226
Hpy188III TCNNGA 2 cut(s) 67, 319
Hpy8I GTNNAC 2 cut(s) 220, 226
Hpy99I CGWCG 2 cut(s) 227, 284
HpyAV CCTTC 2 cut(s) 361, 379
HpyCH4IV ACGT 4 cut(s) 42, 222, 282, 297
HpyCH4V TGCA 1 cut(s) 115
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 79, 419
HpySE526I ACGT 4 cut(s) 42, 222, 282, 297
Hsp92II CATG 1 cut(s) 175
HspAI GCGC 2 cut(s) 48, 420
KroI GCCGGC 1 cut(s) 343
KroNI GCCGGC 1 cut(s) 345
Kzo9I GATC 3 cut(s) 63, 126, 402
LmnI GCTCC 1 cut(s) 195
Lsp1109I GCAGC 2 cut(s) 316, 327
LweI GCATC 1 cut(s) 102
MaeI CTAG 1 cut(s) 439
MaeII ACGT 4 cut(s) 42, 222, 282, 297
MaeIII GTNAC 2 cut(s) 53, 298
MalI GATC 3 cut(s) 65, 128, 404
MboI GATC 3 cut(s) 63, 126, 402
MboII GAAGA 5 cut(s) 28, 43, 307, 409, 412
MflI RGATCY 1 cut(s) 126
MnlI CCTC 5 cut(s) 29, 101, 168, 247, 403
MroNI GCCGGC 1 cut(s) 343
MspR9I CCNGG 5 cut(s) 10, 87, 149, 150, 242
MvaI CCWGG 1 cut(s) 242
MwoI GCNNNNNNNGC 3 cut(s) 45, 79, 419
NaeI GCCGGC 1 cut(s) 345
NciI CCSGG 4 cut(s) 10, 87, 149, 150
NdeII GATC 3 cut(s) 63, 126, 402
NgoMIV GCCGGC 1 cut(s) 343
NlaIII CATG 1 cut(s) 175
NlaIV GGNNCC 3 cut(s) 128, 239, 246
NmeAIII GCCGAG 1 cut(s) 418
NmuCI GTSAC 2 cut(s) 53, 298
NspV TTCGAA 1 cut(s) 383
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PdiI GCCGGC 1 cut(s) 345
PkrI GCNGC 4 cut(s) 249, 306, 342, 419
Psp6I CCWGG 1 cut(s) 240
PspGI CCWGG 1 cut(s) 240
PspN4I GGNNCC 3 cut(s) 128, 239, 246
PspPI GGNCC 2 cut(s) 237, 349
PsuI RGATCY 1 cut(s) 126
RsaI GTAC 2 cut(s) 143, 179
RsaNI GTAC 2 cut(s) 142, 178
Rsr2I CGGWCCG 1 cut(s) 349
RsrII CGGWCCG 1 cut(s) 349
SalI GTCGAC 1 cut(s) 224
SatI GCNGC 4 cut(s) 248, 305, 341, 418
Sau3AI GATC 3 cut(s) 63, 126, 402
Sau96I GGNCC 2 cut(s) 237, 349
ScrFI CCNGG 5 cut(s) 10, 87, 149, 150, 242
SfaNI GCATC 1 cut(s) 102
SfuI TTCGAA 1 cut(s) 383
SinI GGWCC 2 cut(s) 237, 349
SmaI CCCGGG 1 cut(s) 150
SsiI CCGC 8 cut(s) 90, 94, 193, 248, 347, 413, 417, 435
SspMI CTAG 1 cut(s) 439
StyD4I CCNGG 5 cut(s) 8, 85, 147, 148, 240
TaiI ACGT 4 cut(s) 45, 225, 285, 300
TaqI TCGA 3 cut(s) 135, 225, 383
TauI GCSGC 2 cut(s) 250, 420
TseFI GTSAC 2 cut(s) 53, 298
TseI GCWGC 2 cut(s) 304, 340
Tsp45I GTSAC 2 cut(s) 53, 298
TspMI CCCGGG 1 cut(s) 148
VpaK11BI GGWCC 2 cut(s) 237, 349
XmaI CCCGGG 1 cut(s) 148
XmiI GTMKAC 1 cut(s) 225
XspI CTAG 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.