MD16G1102500.v1.1

Belongs to the universal ribosomal protein uL15 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002131
Physical Location & Seq
Forward (+)
7173863 .. 7175952
2090 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1102500.v1.1.491

Sequence Viewer

Length: 441 bp
ATGACGACCCGATTCAAGAAGAACCGCAAGAAGAGAGGCCACGTCAGTGCCGGTCACGGTCGTATCGGCAAGCACCGAAAGCATCCCGGAGGTCGCGGTAACGCCGGAGGCATGCACCACCACCGGATCCTCTTCGACAAGTACCATCCGGGGTATTTCGGTAAAGTCGGTATGAGGTACTTTCACAAGCTTCGCAACAAGTTCTACTGCCCCATCGTGAACGTCGACAAGCTCTGGTCGCTCCTCCCCCAGGAGGCCAAGGACAAGGCCACCAAGGAAAACACGCCGCTCATCGACGTGACCCAGTACGGCTTCTTTAAGGTCCTCGGCAAGGGCGTGTTGCCTCAGAACCAGCCCGTCGTGGTCAAGGCCAAGCTCATTTCCAAGACCGCTGAGAAGAAGATTAAGGAGGCCGGTGGCGCCGTCGTGCTCACCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L27A

Protein Analysis

147

Amino Acids

16.44

Weight (kDa)

10.52

Isoelectric Point (pI)

20.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27A PF00828 72 - 143 1.7e-15 Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 419
AccBSI CCGCTC 1 cut(s) 289
AccI GTMKAC 1 cut(s) 225
AccII CGCG 1 cut(s) 96
AciI CCGC 5 cut(s) 25, 96, 287, 390, 435
AclWI GGATC 2 cut(s) 121, 134
AcyI GRCGYC 1 cut(s) 420
AfaI GTAC 3 cut(s) 143, 179, 308
AfiI CCNNNNNNNGG 3 cut(s) 250, 253, 331
AgsI TTSAA 1 cut(s) 16
AjiI CACGTC 2 cut(s) 43, 298
AjnI CCWGG 1 cut(s) 249
AleI CACNNNNGTG 1 cut(s) 45
AluBI AGCT 3 cut(s) 190, 232, 376
AluI AGCT 3 cut(s) 190, 232, 376
Alw21I GWGCWC 1 cut(s) 432
AlwI GGATC 2 cut(s) 121, 134
AoxI GGCC 5 cut(s) 37, 255, 267, 369, 411
AspLEI GCGC 1 cut(s) 422
AspS9I GGNCC 1 cut(s) 322
AsuC2I CCSGG 2 cut(s) 87, 150
AsuHPI GGTGA 1 cut(s) 424
AvaII GGWCC 1 cut(s) 322
BaeI ACNNNNGTAYC 2 cut(s) 46, 79
BamHI GGATCC 1 cut(s) 126
BanI GGYRCC 1 cut(s) 419
Bbv12I GWGCWC 1 cut(s) 432
BccI CCATC 2 cut(s) 153, 221
BceAI ACGGC 2 cut(s) 325, 407
BciT130I CCWGG 1 cut(s) 251
BcnI CCSGG 2 cut(s) 87, 150
BfoI RGCGCY 1 cut(s) 423
BisI GCNGC 1 cut(s) 287
BlsI GCNGC 1 cut(s) 288
Bme1390I CCNGG 3 cut(s) 87, 150, 251
Bme18I GGWCC 1 cut(s) 322
BmgBI CACGTC 2 cut(s) 43, 298
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 2 cut(s) 128, 421
BmrFI CCNGG 3 cut(s) 87, 150, 251
BmrI ACTGGG 1 cut(s) 298
BmsI GCATC 1 cut(s) 91
BmuI ACTGGG 1 cut(s) 298
BpuMI CCSGG 2 cut(s) 87, 150
BsaHI GRCGYC 1 cut(s) 420
BsaJI CCNNGG 5 cut(s) 149, 249, 258, 273, 325
BsaWI WCCGGW 1 cut(s) 123
Bsc4I CCNNNNNNNGG 3 cut(s) 250, 253, 331
Bse118I RCCGGY 2 cut(s) 50, 413
Bse1I ACTGG 1 cut(s) 304
BseBI CCWGG 1 cut(s) 251
BseDI CCNNGG 5 cut(s) 149, 249, 258, 273, 325
BseGI GGATG 2 cut(s) 82, 145
BseLI CCNNNNNNNGG 3 cut(s) 250, 253, 331
BseMII CTCAG 2 cut(s) 359, 384
BseNI ACTGG 1 cut(s) 304
BseRI GAGGAG 1 cut(s) 233
Bsh1236I CGCG 1 cut(s) 96
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 5 cut(s) 39, 257, 269, 371, 413
BshNI GGYRCC 1 cut(s) 419
BsiEI CGRYCG 1 cut(s) 61
BsiHKAI GWGCWC 1 cut(s) 432
BsiSI CCGG 6 cut(s) 51, 87, 105, 124, 149, 414
BslI CCNNNNNNNGG 3 cut(s) 250, 253, 331
BsnI GGCC 5 cut(s) 39, 257, 269, 371, 413
Bsp1286I GDGCHC 1 cut(s) 432
Bsp143I GATC 1 cut(s) 126
BspACI CCGC 5 cut(s) 25, 96, 287, 390, 435
BspANI GGCC 5 cut(s) 39, 257, 269, 371, 413
BspCNI CTCAG 2 cut(s) 358, 385
BspFNI CGCG 1 cut(s) 96
BspLI GGNNCC 2 cut(s) 128, 421
BspPI GGATC 2 cut(s) 121, 134
BspT107I GGYRCC 1 cut(s) 419
BsrBI CCGCTC 1 cut(s) 289
BsrFI RCCGGY 2 cut(s) 50, 413
BsrI ACTGG 1 cut(s) 304
BssAI RCCGGY 2 cut(s) 50, 413
BssECI CCNNGG 5 cut(s) 149, 249, 258, 273, 325
BssMI GATC 1 cut(s) 126
BssNI GRCGYC 1 cut(s) 420
BssT1I CCWWGG 2 cut(s) 258, 273
Bst2UI CCWGG 1 cut(s) 251
Bst4CI ACNGT 1 cut(s) 59
Bst6I CTCTTC 2 cut(s) 26, 137
BstACI GRCGYC 1 cut(s) 420
BstC8I GCNNGC 2 cut(s) 71, 113
BstDEI CTNAG 3 cut(s) 345, 393, 438
BstENI CCTNNNNNAGG 2 cut(s) 248, 329
BstF5I GGATG 2 cut(s) 82, 145
BstFNI CGCG 1 cut(s) 96
BstH2I RGCGCY 1 cut(s) 423
BstHHI GCGC 1 cut(s) 422
BstKTI GATC 1 cut(s) 129
BstMBI GATC 1 cut(s) 126
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 3 cut(s) 79, 238, 419
BstNI CCWGG 1 cut(s) 251
BstNSI RCATGY 1 cut(s) 115
BstSCI CCNGG 3 cut(s) 85, 148, 249
BstUI CGCG 1 cut(s) 96
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
BsuRI GGCC 5 cut(s) 39, 257, 269, 371, 413
BtrI CACGTC 2 cut(s) 43, 298
BtsCI GGATG 2 cut(s) 82, 145
BtsIMutI CAGTG 1 cut(s) 52
Cac8I GCNNGC 2 cut(s) 71, 113
CfoI GCGC 1 cut(s) 422
Cfr10I RCCGGY 2 cut(s) 50, 413
Cfr13I GGNCC 1 cut(s) 322
Csp6I GTAC 3 cut(s) 142, 178, 307
CviAII CATG 1 cut(s) 112
CviQI GTAC 3 cut(s) 142, 178, 307
DdeI CTNAG 3 cut(s) 345, 393, 438
DinI GGCGCC 1 cut(s) 421
DpnI GATC 1 cut(s) 128
DpnII GATC 1 cut(s) 126
Eam1104I CTCTTC 2 cut(s) 26, 137
EarI CTCTTC 2 cut(s) 26, 137
Eco130I CCWWGG 2 cut(s) 258, 273
Eco47I GGWCC 1 cut(s) 322
EcoNI CCTNNNNNAGG 2 cut(s) 248, 329
EcoO109I RGGNCCY 1 cut(s) 322
EcoRII CCWGG 1 cut(s) 249
EcoT14I CCWWGG 2 cut(s) 258, 273
EgeI GGCGCC 1 cut(s) 421
EheI GGCGCC 1 cut(s) 421
ErhI CCWWGG 2 cut(s) 258, 273
FaeI CATG 1 cut(s) 115
FaiI YATR 2 cut(s) 113, 173
FatI CATG 1 cut(s) 111
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 1 cut(s) 287
FokI GGATG 2 cut(s) 69, 132
Fsp4HI GCNGC 1 cut(s) 287
GlaI GCGC 1 cut(s) 421
GluI GCNGC 1 cut(s) 287
HaeII RGCGCY 1 cut(s) 423
HaeIII GGCC 5 cut(s) 39, 257, 269, 371, 413
HapII CCGG 6 cut(s) 51, 87, 105, 124, 149, 414
HhaI GCGC 1 cut(s) 422
Hin1I GRCGYC 1 cut(s) 420
Hin1II CATG 1 cut(s) 115
Hin6I GCGC 1 cut(s) 420
HinP1I GCGC 1 cut(s) 420
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 1 cut(s) 12
HpaII CCGG 6 cut(s) 51, 87, 105, 124, 149, 414
HphI GGTGA 1 cut(s) 424
Hpy166II GTNNAC 2 cut(s) 220, 226
Hpy188I TCNGA 1 cut(s) 348
Hpy188III TCNNGA 2 cut(s) 16, 217
Hpy8I GTNNAC 2 cut(s) 220, 226
Hpy99I CGWCG 4 cut(s) 227, 299, 362, 428
HpyCH4III ACNGT 1 cut(s) 59
HpyCH4IV ACGT 3 cut(s) 42, 222, 297
HpyCH4V TGCA 1 cut(s) 115
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 238, 419
HpyF3I CTNAG 3 cut(s) 345, 393, 438
HpySE526I ACGT 3 cut(s) 42, 222, 297
Hsp92I GRCGYC 1 cut(s) 420
Hsp92II CATG 1 cut(s) 115
HspAI GCGC 1 cut(s) 420
KasI GGCGCC 1 cut(s) 419
Kzo9I GATC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 246
LweI GCATC 1 cut(s) 91
MaeII ACGT 3 cut(s) 42, 222, 297
MaeIII GTNAC 3 cut(s) 53, 98, 298
MalI GATC 1 cut(s) 128
MbiI CCGCTC 1 cut(s) 289
MboI GATC 1 cut(s) 126
MboII GAAGA 5 cut(s) 31, 43, 124, 409, 412
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 1 cut(s) 432
Mly113I GGCGCC 1 cut(s) 420
MseI TTAA 2 cut(s) 318, 405
MslI CAYNNNNRTG 2 cut(s) 45, 296
MspA1I CMGCKG 1 cut(s) 392
MspI CCGG 6 cut(s) 51, 87, 105, 124, 149, 414
MspR9I CCNGG 3 cut(s) 87, 150, 251
MvaI CCWGG 1 cut(s) 251
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 3 cut(s) 79, 238, 419
NarI GGCGCC 1 cut(s) 420
NciI CCSGG 2 cut(s) 87, 150
NdeII GATC 1 cut(s) 126
NlaIII CATG 1 cut(s) 115
NlaIV GGNNCC 2 cut(s) 128, 421
NmeAIII GCCGAG 1 cut(s) 306
NmuCI GTSAC 2 cut(s) 53, 298
NspI RCATGY 1 cut(s) 115
OliI CACNNNNGTG 1 cut(s) 45
PaeI GCATGC 1 cut(s) 115
PcsI WCGNNNNNNNCGW 2 cut(s) 222, 333
PfeI GAWTC 1 cut(s) 12
PfoI TCCNGGA 1 cut(s) 85
PkrI GCNGC 1 cut(s) 288
PluTI GGCGCC 1 cut(s) 423
PpuMI RGGWCCY 1 cut(s) 322
Psp5II RGGWCCY 1 cut(s) 322
Psp6I CCWGG 1 cut(s) 249
PspGI CCWGG 1 cut(s) 249
PspN4I GGNNCC 2 cut(s) 128, 421
PspPI GGNCC 1 cut(s) 322
PspPPI RGGWCCY 1 cut(s) 322
PsuI RGATCY 1 cut(s) 126
RsaI GTAC 3 cut(s) 143, 179, 308
RsaNI GTAC 3 cut(s) 142, 178, 307
RseI CAYNNNNRTG 2 cut(s) 45, 296
SalI GTCGAC 1 cut(s) 224
SaqAI TTAA 2 cut(s) 318, 405
SatI GCNGC 1 cut(s) 287
Sau3AI GATC 1 cut(s) 126
Sau96I GGNCC 1 cut(s) 322
ScrFI CCNGG 3 cut(s) 87, 150, 251
SduI GDGCHC 1 cut(s) 432
SetI ASST 9 cut(s) 45, 94, 179, 192, 225, 234, 300, 324, 378
SfaNI GCATC 1 cut(s) 91
SfoI GGCGCC 1 cut(s) 421
SinI GGWCC 1 cut(s) 322
SmiMI CAYNNNNRTG 2 cut(s) 45, 296
SphI GCATGC 1 cut(s) 115
SsiI CCGC 5 cut(s) 25, 96, 287, 390, 435
SspDI GGCGCC 1 cut(s) 419
StyD4I CCNGG 3 cut(s) 85, 148, 249
StyI CCWWGG 2 cut(s) 258, 273
TaaI ACNGT 1 cut(s) 59
TaiI ACGT 3 cut(s) 45, 225, 300
TaqI TCGA 3 cut(s) 135, 225, 294
TauI GCSGC 1 cut(s) 289
TfiI GAWTC 1 cut(s) 12
Tru1I TTAA 2 cut(s) 318, 405
Tru9I TTAA 2 cut(s) 318, 405
TscAI CASTG 1 cut(s) 52
TseFI GTSAC 2 cut(s) 53, 298
Tsp45I GTSAC 2 cut(s) 53, 298
TspRI CASTG 1 cut(s) 52
VpaK11BI GGWCC 1 cut(s) 322
XagI CCTNNNNNAGG 2 cut(s) 248, 329
XceI RCATGY 1 cut(s) 115
XmiI GTMKAC 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.