pycom13g08910

Belongs to the universal ribosomal protein uL15 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0002131
Physical Location & Seq
Forward (+)
5934484 .. 5934924
441 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g08910.1

Sequence Viewer

Length: 441 bp
ATGACGACCCGATTCAAGAAGAACCGCAAGAAGAGAGGCCACGTCAGCGCCGGTCACGGTCGTATCGGCAAGCACCGCAAGCATCCCGGAGGTCGCGGTAACGCCGGAGGCATGCACCACCACCGGATCCTCTTCGACAAGTACCATCCTGGGTATTTCGGTAAAGTCGGTATGAGGTACTTTCACAAGCTCCGCAACAAGTTCTACTGCCCCATCGTCAACGTCGACAAGCTCTGGTCGCTTCTCCCTCAGGAGGCCAAGGACAAGGCCACCAAGGACAACGCCCCGCTCATCGATGTGACTCAGTACGGCTTCTTCAAGGTCCTAGGCAAGGGCGTGTTGCCGCAGAACCAGCCCGTCGTGGTGAAGGCCAAGCTCATCTCCAAGACCGCAGAGAAGAAAATTAAGGAGGCCGGTGGCGCCGTCGTGCTCACCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L27A

Protein Analysis

147

Amino Acids

16.4

Weight (kDa)

10.52

Isoelectric Point (pI)

22.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27A PF00828 72 - 143 1.9e-15 Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 419
AccBSI CCGCTC 1 cut(s) 289
AccI GTMKAC 1 cut(s) 225
AccII CGCG 1 cut(s) 96
AciI CCGC 8 cut(s) 25, 76, 96, 193, 287, 344, 390, 435
AclWI GGATC 2 cut(s) 121, 134
AcyI GRCGYC 1 cut(s) 420
AfaI GTAC 3 cut(s) 143, 179, 308
AfiI CCNNNNNNNGG 2 cut(s) 253, 331
AgsI TTSAA 2 cut(s) 16, 319
AjiI CACGTC 1 cut(s) 43
AjnI CCWGG 1 cut(s) 148
AluBI AGCT 3 cut(s) 190, 232, 376
AluI AGCT 3 cut(s) 190, 232, 376
Alw21I GWGCWC 1 cut(s) 432
AlwI GGATC 2 cut(s) 121, 134
AoxI GGCC 5 cut(s) 37, 255, 267, 369, 411
AspA2I CCTAGG 1 cut(s) 325
AspLEI GCGC 2 cut(s) 50, 422
AspS9I GGNCC 1 cut(s) 322
AsuC2I CCSGG 1 cut(s) 87
AsuHPI GGTGA 2 cut(s) 376, 424
AvaII GGWCC 1 cut(s) 322
AvrII CCTAGG 1 cut(s) 325
AxyI CCTNAGG 1 cut(s) 249
BaeI ACNNNNGTAYC 2 cut(s) 46, 79
BamHI GGATCC 1 cut(s) 126
BanI GGYRCC 1 cut(s) 419
BarI GAAGNNNNNNTAC 2 cut(s) 299, 331
Bbv12I GWGCWC 1 cut(s) 432
BccI CCATC 2 cut(s) 153, 221
BceAI ACGGC 2 cut(s) 325, 407
BciT130I CCWGG 1 cut(s) 150
BcnI CCSGG 1 cut(s) 87
BfaI CTAG 1 cut(s) 326
BfoI RGCGCY 2 cut(s) 51, 423
BisI GCNGC 1 cut(s) 344
BlnI CCTAGG 1 cut(s) 325
BlsI GCNGC 1 cut(s) 345
Bme1390I CCNGG 2 cut(s) 87, 150
Bme18I GGWCC 1 cut(s) 322
BmgBI CACGTC 1 cut(s) 43
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 2 cut(s) 128, 421
BmrFI CCNGG 2 cut(s) 87, 150
BmsI GCATC 1 cut(s) 91
BpuMI CCSGG 1 cut(s) 87
Bsa29I ATCGAT 1 cut(s) 294
BsaHI GRCGYC 1 cut(s) 420
BsaJI CCNNGG 4 cut(s) 149, 258, 273, 325
BsaWI WCCGGW 1 cut(s) 123
Bsc4I CCNNNNNNNGG 2 cut(s) 253, 331
Bse118I RCCGGY 2 cut(s) 50, 413
Bse21I CCTNAGG 1 cut(s) 249
BseBI CCWGG 1 cut(s) 150
BseCI ATCGAT 1 cut(s) 294
BseDI CCNNGG 4 cut(s) 149, 258, 273, 325
BseGI GGATG 2 cut(s) 82, 145
BseLI CCNNNNNNNGG 2 cut(s) 253, 331
BseMII CTCAG 2 cut(s) 263, 317
Bsh1236I CGCG 1 cut(s) 96
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 5 cut(s) 39, 257, 269, 371, 413
BshNI GGYRCC 1 cut(s) 419
BshVI ATCGAT 1 cut(s) 294
BsiEI CGRYCG 1 cut(s) 61
BsiHKAI GWGCWC 1 cut(s) 432
BsiSI CCGG 5 cut(s) 51, 87, 105, 124, 414
BslI CCNNNNNNNGG 2 cut(s) 253, 331
BsnI GGCC 5 cut(s) 39, 257, 269, 371, 413
Bsp1286I GDGCHC 1 cut(s) 432
Bsp143I GATC 1 cut(s) 126
BspACI CCGC 8 cut(s) 25, 76, 96, 193, 287, 344, 390, 435
BspANI GGCC 5 cut(s) 39, 257, 269, 371, 413
BspCNI CTCAG 2 cut(s) 262, 316
BspDI ATCGAT 1 cut(s) 294
BspFNI CGCG 1 cut(s) 96
BspLI GGNNCC 2 cut(s) 128, 421
BspPI GGATC 2 cut(s) 121, 134
BspT107I GGYRCC 1 cut(s) 419
BsrBI CCGCTC 1 cut(s) 289
BsrFI RCCGGY 2 cut(s) 50, 413
BssAI RCCGGY 2 cut(s) 50, 413
BssECI CCNNGG 4 cut(s) 149, 258, 273, 325
BssMI GATC 1 cut(s) 126
BssNI GRCGYC 1 cut(s) 420
BssT1I CCWWGG 3 cut(s) 258, 273, 325
Bst2UI CCWGG 1 cut(s) 150
Bst4CI ACNGT 1 cut(s) 59
Bst6I CTCTTC 2 cut(s) 26, 137
BstACI GRCGYC 1 cut(s) 420
BstC8I GCNNGC 3 cut(s) 71, 80, 113
BstDEI CTNAG 3 cut(s) 249, 303, 438
BstENI CCTNNNNNAGG 1 cut(s) 329
BstF5I GGATG 2 cut(s) 82, 145
BstFNI CGCG 1 cut(s) 96
BstH2I RGCGCY 2 cut(s) 51, 423
BstHHI GCGC 2 cut(s) 50, 422
BstKTI GATC 1 cut(s) 129
BstMBI GATC 1 cut(s) 126
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 6 cut(s) 45, 75, 79, 238, 352, 419
BstNI CCWGG 1 cut(s) 150
BstNSI RCATGY 1 cut(s) 115
BstSCI CCNGG 2 cut(s) 85, 148
BstUI CGCG 1 cut(s) 96
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
Bsu15I ATCGAT 1 cut(s) 294
Bsu36I CCTNAGG 1 cut(s) 249
BsuRI GGCC 5 cut(s) 39, 257, 269, 371, 413
BsuTUI ATCGAT 1 cut(s) 294
BtrI CACGTC 1 cut(s) 43
BtsCI GGATG 2 cut(s) 82, 145
Cac8I GCNNGC 3 cut(s) 71, 80, 113
CfoI GCGC 2 cut(s) 50, 422
Cfr10I RCCGGY 2 cut(s) 50, 413
Cfr13I GGNCC 1 cut(s) 322
ClaI ATCGAT 1 cut(s) 294
Csp6I GTAC 3 cut(s) 142, 178, 307
CviAII CATG 1 cut(s) 112
CviQI GTAC 3 cut(s) 142, 178, 307
DdeI CTNAG 3 cut(s) 249, 303, 438
DinI GGCGCC 1 cut(s) 421
DpnI GATC 1 cut(s) 128
DpnII GATC 1 cut(s) 126
Eam1104I CTCTTC 2 cut(s) 26, 137
EarI CTCTTC 2 cut(s) 26, 137
Eco130I CCWWGG 3 cut(s) 258, 273, 325
Eco47I GGWCC 1 cut(s) 322
Eco81I CCTNAGG 1 cut(s) 249
EcoNI CCTNNNNNAGG 1 cut(s) 329
EcoO109I RGGNCCY 1 cut(s) 322
EcoRII CCWGG 1 cut(s) 148
EcoT14I CCWWGG 3 cut(s) 258, 273, 325
EgeI GGCGCC 1 cut(s) 421
EheI GGCGCC 1 cut(s) 421
ErhI CCWWGG 3 cut(s) 258, 273, 325
FaeI CATG 1 cut(s) 115
FaiI YATR 2 cut(s) 113, 173
FatI CATG 1 cut(s) 111
FauI CCCGC 1 cut(s) 294
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 1 cut(s) 344
FokI GGATG 2 cut(s) 69, 132
Fsp4HI GCNGC 1 cut(s) 344
FspBI CTAG 1 cut(s) 326
GlaI GCGC 2 cut(s) 49, 421
GluI GCNGC 1 cut(s) 344
HaeII RGCGCY 2 cut(s) 51, 423
HaeIII GGCC 5 cut(s) 39, 257, 269, 371, 413
HapII CCGG 5 cut(s) 51, 87, 105, 124, 414
HhaI GCGC 2 cut(s) 50, 422
Hin1I GRCGYC 1 cut(s) 420
Hin1II CATG 1 cut(s) 115
Hin6I GCGC 2 cut(s) 48, 420
HinP1I GCGC 2 cut(s) 48, 420
HincII GTYRAC 2 cut(s) 220, 226
HindII GTYRAC 2 cut(s) 220, 226
HinfI GANTC 2 cut(s) 12, 301
HpaII CCGG 5 cut(s) 51, 87, 105, 124, 414
HphI GGTGA 2 cut(s) 376, 424
Hpy166II GTNNAC 2 cut(s) 220, 226
Hpy188III TCNNGA 2 cut(s) 16, 251
Hpy8I GTNNAC 2 cut(s) 220, 226
Hpy99I CGWCG 3 cut(s) 227, 362, 428
HpyAV CCTTC 1 cut(s) 361
HpyCH4III ACNGT 1 cut(s) 59
HpyCH4IV ACGT 2 cut(s) 42, 222
HpyCH4V TGCA 1 cut(s) 115
HpyF10VI GCNNNNNNNGC 6 cut(s) 45, 75, 79, 238, 352, 419
HpyF3I CTNAG 3 cut(s) 249, 303, 438
HpySE526I ACGT 2 cut(s) 42, 222
Hsp92I GRCGYC 1 cut(s) 420
Hsp92II CATG 1 cut(s) 115
HspAI GCGC 2 cut(s) 48, 420
KasI GGCGCC 1 cut(s) 419
Kzo9I GATC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 195
LweI GCATC 1 cut(s) 91
MaeI CTAG 1 cut(s) 326
MaeII ACGT 2 cut(s) 42, 222
MaeIII GTNAC 3 cut(s) 53, 98, 298
MalI GATC 1 cut(s) 128
MbiI CCGCTC 1 cut(s) 289
MboI GATC 1 cut(s) 126
MboII GAAGA 5 cut(s) 31, 43, 124, 307, 409
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 1 cut(s) 402
Mly113I GGCGCC 1 cut(s) 420
MlyI GAGTC 1 cut(s) 295
MnlI CCTC 8 cut(s) 29, 83, 101, 140, 168, 247, 258, 403
MseI TTAA 1 cut(s) 405
MslI CAYNNNNRTG 1 cut(s) 296
MspI CCGG 5 cut(s) 51, 87, 105, 124, 414
MspR9I CCNGG 2 cut(s) 87, 150
MvaI CCWGG 1 cut(s) 150
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 6 cut(s) 45, 75, 79, 238, 352, 419
NarI GGCGCC 1 cut(s) 420
NciI CCSGG 1 cut(s) 87
NdeII GATC 1 cut(s) 126
NlaIII CATG 1 cut(s) 115
NlaIV GGNNCC 2 cut(s) 128, 421
NmuCI GTSAC 2 cut(s) 53, 298
NspI RCATGY 1 cut(s) 115
PaeI GCATGC 1 cut(s) 115
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PfeI GAWTC 1 cut(s) 12
PfoI TCCNGGA 1 cut(s) 85
PkrI GCNGC 1 cut(s) 345
PleI GAGTC 1 cut(s) 295
PluTI GGCGCC 1 cut(s) 423
PpsI GAGTC 1 cut(s) 295
PpuMI RGGWCCY 1 cut(s) 322
Psp5II RGGWCCY 1 cut(s) 322
Psp6I CCWGG 1 cut(s) 148
PspGI CCWGG 1 cut(s) 148
PspN4I GGNNCC 2 cut(s) 128, 421
PspPI GGNCC 1 cut(s) 322
PspPPI RGGWCCY 1 cut(s) 322
PsuI RGATCY 1 cut(s) 126
RsaI GTAC 3 cut(s) 143, 179, 308
RsaNI GTAC 3 cut(s) 142, 178, 307
RseI CAYNNNNRTG 1 cut(s) 296
SalI GTCGAC 1 cut(s) 224
SaqAI TTAA 1 cut(s) 405
SatI GCNGC 1 cut(s) 344
Sau3AI GATC 1 cut(s) 126
Sau96I GGNCC 1 cut(s) 322
SchI GAGTC 1 cut(s) 295
ScrFI CCNGG 2 cut(s) 87, 150
SduI GDGCHC 1 cut(s) 432
SetI ASST 8 cut(s) 45, 94, 179, 192, 225, 234, 324, 378
SfaNI GCATC 1 cut(s) 91
SfoI GGCGCC 1 cut(s) 421
SinI GGWCC 1 cut(s) 322
SmiMI CAYNNNNRTG 1 cut(s) 296
SphI GCATGC 1 cut(s) 115
Sse9I AATT 1 cut(s) 402
SsiI CCGC 8 cut(s) 25, 76, 96, 193, 287, 344, 390, 435
SspDI GGCGCC 1 cut(s) 419
SspMI CTAG 1 cut(s) 326
StyD4I CCNGG 2 cut(s) 85, 148
StyI CCWWGG 3 cut(s) 258, 273, 325
TaaI ACNGT 1 cut(s) 59
TaiI ACGT 2 cut(s) 45, 225
TaqI TCGA 3 cut(s) 135, 225, 294
TasI AATT 1 cut(s) 402
TauI GCSGC 1 cut(s) 346
TfiI GAWTC 1 cut(s) 12
Tru1I TTAA 1 cut(s) 405
Tru9I TTAA 1 cut(s) 405
TseFI GTSAC 2 cut(s) 53, 298
Tsp45I GTSAC 2 cut(s) 53, 298
VpaK11BI GGWCC 1 cut(s) 322
XagI CCTNNNNNAGG 1 cut(s) 329
XceI RCATGY 1 cut(s) 115
XmaJI CCTAGG 1 cut(s) 325
XmiI GTMKAC 1 cut(s) 225
XspI CTAG 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.