pycom17g02200

Belongs to the universal ribosomal protein uL15 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002131
Physical Location & Seq
Forward (+)
1383833 .. 1384282
450 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g02200.1

Sequence Viewer

Length: 450 bp
ATGACGACGAGGTTCAAGAAGAACCGCAAGAAGCGCGGCCACGTCAGCGCCGGCCACGGGCGTATCGGCAAGCACCGCAAGCACCCCGGCGGTCGCGGTAACGCCGGAGGTATGCACCACCACCGGATCCTTTTCGACAAGTACCATCCAGGGTATTTCGGTAAAGTCGGTATGAGGTACTTCCACAGGCTGCGCAACAAGTTCCATTGCCCGATCGTCAACCTCGACAAGCTGTGGTCGCTTGTACCCCAGGAGGTGAAGGACAAGGCCGCCGCCGCCAAGGGAGGAAGCGAGGCGCCGTTGCTCGACGTCACTCAGTTCGGGTACTTCAAGGTGTTGGGGAAGGGCGTGTTACCGTCACAGCCATTGGTGGTGAAGGCCAAGCTGATTTCAAAGACTGCCGAGAAGAAGATCAAGGAGGCTGGTGGCGCCGTCGTGCTCACCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L27A

Protein Analysis

150

Amino Acids

16.5

Weight (kDa)

10.67

Isoelectric Point (pI)

21.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L27A PF00828 72 - 146 3.1e-15 Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 312
Acc16I TGCGCA 1 cut(s) 194
AccB1I GGYRCC 2 cut(s) 295, 428
AccII CGCG 2 cut(s) 36, 96
AciI CCGC 9 cut(s) 25, 36, 76, 90, 96, 270, 273, 276, 444
AclWI GGATC 2 cut(s) 121, 134
AcoI YGGCCR 2 cut(s) 37, 52
AcyI GRCGYC 3 cut(s) 296, 309, 429
AfaI GTAC 4 cut(s) 143, 179, 246, 326
AfiI CCNNNNNNNGG 1 cut(s) 57
AgsI TTSAA 3 cut(s) 16, 331, 393
AjiI CACGTC 1 cut(s) 43
AjnI CCWGG 2 cut(s) 148, 249
AluBI AGCT 2 cut(s) 232, 385
AluI AGCT 2 cut(s) 232, 385
Alw21I GWGCWC 1 cut(s) 441
AlwI GGATC 2 cut(s) 121, 134
AoxI GGCC 4 cut(s) 37, 52, 267, 378
ApeKI GCWGC 1 cut(s) 190
AspLEI GCGC 5 cut(s) 36, 50, 195, 298, 431
AsuC2I CCSGG 1 cut(s) 87
AsuHPI GGTGA 3 cut(s) 268, 385, 433
BaeI ACNNNNGTAYC 2 cut(s) 46, 79
BamHI GGATCC 1 cut(s) 126
BanI GGYRCC 2 cut(s) 295, 428
Bbv12I GWGCWC 1 cut(s) 441
BbvI GCAGC 1 cut(s) 177
BccI CCATC 1 cut(s) 153
BceAI ACGGC 2 cut(s) 283, 416
BciT130I CCWGG 2 cut(s) 150, 251
BcnI CCSGG 1 cut(s) 87
BfoI RGCGCY 3 cut(s) 51, 299, 432
BisI GCNGC 5 cut(s) 37, 191, 270, 273, 276
BlsI GCNGC 5 cut(s) 38, 192, 271, 274, 277
Bme1390I CCNGG 3 cut(s) 87, 150, 251
BmgBI CACGTC 1 cut(s) 43
BmiI GGNNCC 3 cut(s) 128, 297, 430
BmrFI CCNGG 3 cut(s) 87, 150, 251
BpuMI CCSGG 1 cut(s) 87
BsaHI GRCGYC 3 cut(s) 296, 309, 429
BsaJI CCNNGG 5 cut(s) 55, 85, 149, 249, 279
BsaWI WCCGGW 1 cut(s) 123
Bsc4I CCNNNNNNNGG 1 cut(s) 57
Bse118I RCCGGY 1 cut(s) 50
Bse3DI GCAATG 1 cut(s) 205
BseBI CCWGG 2 cut(s) 150, 251
BseDI CCNNGG 5 cut(s) 55, 85, 149, 249, 279
BseGI GGATG 1 cut(s) 145
BseLI CCNNNNNNNGG 1 cut(s) 57
BseMI GCAATG 1 cut(s) 205
BseMII CTCAG 1 cut(s) 329
BseXI GCAGC 1 cut(s) 177
Bsh1236I CGCG 2 cut(s) 36, 96
Bsh1285I CGRYCG 2 cut(s) 94, 216
BshFI GGCC 4 cut(s) 39, 54, 269, 380
BshNI GGYRCC 2 cut(s) 295, 428
BsiEI CGRYCG 2 cut(s) 94, 216
BsiHKAI GWGCWC 1 cut(s) 441
BsiSI CCGG 4 cut(s) 51, 87, 105, 124
BslI CCNNNNNNNGG 1 cut(s) 57
BsnI GGCC 4 cut(s) 39, 54, 269, 380
Bsp1286I GDGCHC 1 cut(s) 441
Bsp143I GATC 3 cut(s) 126, 213, 411
BspACI CCGC 9 cut(s) 25, 36, 76, 90, 96, 270, 273, 276, 444
BspANI GGCC 4 cut(s) 39, 54, 269, 380
BspCNI CTCAG 1 cut(s) 328
BspFNI CGCG 2 cut(s) 36, 96
BspLI GGNNCC 3 cut(s) 128, 297, 430
BspPI GGATC 2 cut(s) 121, 134
BspT107I GGYRCC 2 cut(s) 295, 428
BsrDI GCAATG 1 cut(s) 205
BsrFI RCCGGY 1 cut(s) 50
BssAI RCCGGY 1 cut(s) 50
BssECI CCNNGG 5 cut(s) 55, 85, 149, 249, 279
BssMI GATC 3 cut(s) 126, 213, 411
BssNI GRCGYC 3 cut(s) 296, 309, 429
BssT1I CCWWGG 1 cut(s) 279
Bst2UI CCWGG 2 cut(s) 150, 251
Bst4CI ACNGT 1 cut(s) 357
BstACI GRCGYC 3 cut(s) 296, 309, 429
BstC8I GCNNGC 3 cut(s) 52, 71, 80
BstDEI CTNAG 2 cut(s) 315, 447
BstDSI CCRYGG 1 cut(s) 55
BstF5I GGATG 1 cut(s) 145
BstFNI CGCG 2 cut(s) 36, 96
BstH2I RGCGCY 3 cut(s) 51, 299, 432
BstHHI GCGC 5 cut(s) 36, 50, 195, 298, 431
BstKTI GATC 3 cut(s) 129, 216, 414
BstMBI GATC 3 cut(s) 126, 213, 411
BstMCI CGRYCG 2 cut(s) 94, 216
BstMWI GCNNNNNNNGC 7 cut(s) 33, 45, 75, 79, 238, 275, 428
BstNI CCWGG 2 cut(s) 150, 251
BstSCI CCNGG 3 cut(s) 85, 148, 249
BstUI CGCG 2 cut(s) 36, 96
BstV1I GCAGC 1 cut(s) 177
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
BsuRI GGCC 4 cut(s) 39, 54, 269, 380
BtgI CCRYGG 1 cut(s) 55
BtrI CACGTC 1 cut(s) 43
BtsCI GGATG 1 cut(s) 145
Cac8I GCNNGC 3 cut(s) 52, 71, 80
CfoI GCGC 5 cut(s) 36, 50, 195, 298, 431
Cfr10I RCCGGY 1 cut(s) 50
Csp6I GTAC 4 cut(s) 142, 178, 245, 325
CviJI RGCY 9 cut(s) 39, 54, 190, 232, 269, 364, 380, 385, 422
CviKI_1 RGCY 9 cut(s) 39, 54, 190, 232, 269, 364, 380, 385, 422
CviQI GTAC 4 cut(s) 142, 178, 245, 325
DdeI CTNAG 2 cut(s) 315, 447
DinI GGCGCC 2 cut(s) 297, 430
DpnI GATC 3 cut(s) 128, 215, 413
DpnII GATC 3 cut(s) 126, 213, 411
EaeI YGGCCR 2 cut(s) 37, 52
Eco130I CCWWGG 1 cut(s) 279
EcoRII CCWGG 2 cut(s) 148, 249
EcoT14I CCWWGG 1 cut(s) 279
EgeI GGCGCC 2 cut(s) 297, 430
EheI GGCGCC 2 cut(s) 297, 430
ErhI CCWWGG 1 cut(s) 279
FaiI YATR 2 cut(s) 113, 173
Fnu4HI GCNGC 5 cut(s) 37, 191, 270, 273, 276
FokI GGATG 1 cut(s) 132
Fsp4HI GCNGC 5 cut(s) 37, 191, 270, 273, 276
FspI TGCGCA 1 cut(s) 194
GlaI GCGC 5 cut(s) 35, 49, 194, 297, 430
GluI GCNGC 5 cut(s) 37, 191, 270, 273, 276
HaeII RGCGCY 3 cut(s) 51, 299, 432
HaeIII GGCC 4 cut(s) 39, 54, 269, 380
HapII CCGG 4 cut(s) 51, 87, 105, 124
HhaI GCGC 5 cut(s) 36, 50, 195, 298, 431
Hin1I GRCGYC 3 cut(s) 296, 309, 429
Hin6I GCGC 5 cut(s) 34, 48, 193, 296, 429
HinP1I GCGC 5 cut(s) 34, 48, 193, 296, 429
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HpaII CCGG 4 cut(s) 51, 87, 105, 124
HphI GGTGA 3 cut(s) 268, 385, 433
Hpy166II GTNNAC 1 cut(s) 220
Hpy188III TCNNGA 1 cut(s) 16
Hpy8I GTNNAC 1 cut(s) 220
Hpy99I CGWCG 3 cut(s) 10, 311, 437
HpyAV CCTTC 3 cut(s) 253, 337, 370
HpyCH4III ACNGT 1 cut(s) 357
HpyCH4IV ACGT 2 cut(s) 42, 309
HpyCH4V TGCA 1 cut(s) 115
HpyF10VI GCNNNNNNNGC 7 cut(s) 33, 45, 75, 79, 238, 275, 428
HpyF3I CTNAG 2 cut(s) 315, 447
HpySE526I ACGT 2 cut(s) 42, 309
Hsp92I GRCGYC 3 cut(s) 296, 309, 429
HspAI GCGC 5 cut(s) 34, 48, 193, 296, 429
KasI GGCGCC 2 cut(s) 295, 428
KroI GCCGGC 1 cut(s) 50
KroNI GCCGGC 1 cut(s) 52
Kzo9I GATC 3 cut(s) 126, 213, 411
Lsp1109I GCAGC 1 cut(s) 177
MaeII ACGT 2 cut(s) 42, 309
MaeIII GTNAC 4 cut(s) 98, 310, 351, 357
MalI GATC 3 cut(s) 128, 215, 413
MboI GATC 3 cut(s) 126, 213, 411
MboII GAAGA 3 cut(s) 31, 418, 421
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 1 cut(s) 441
Mly113I GGCGCC 2 cut(s) 296, 429
MnlI CCTC 8 cut(s) 3, 101, 168, 233, 247, 278, 286, 412
MroNI GCCGGC 1 cut(s) 50
MspI CCGG 4 cut(s) 51, 87, 105, 124
MspR9I CCNGG 3 cut(s) 87, 150, 251
MvaI CCWGG 2 cut(s) 150, 251
MvnI CGCG 2 cut(s) 36, 96
MwoI GCNNNNNNNGC 7 cut(s) 33, 45, 75, 79, 238, 275, 428
NaeI GCCGGC 1 cut(s) 52
NarI GGCGCC 2 cut(s) 296, 429
NciI CCSGG 1 cut(s) 87
NdeII GATC 3 cut(s) 126, 213, 411
NgoMIV GCCGGC 1 cut(s) 50
NlaIV GGNNCC 3 cut(s) 128, 297, 430
NmeAIII GCCGAG 1 cut(s) 427
NmuCI GTSAC 2 cut(s) 310, 357
NsbI TGCGCA 1 cut(s) 194
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PdiI GCCGGC 1 cut(s) 52
PkrI GCNGC 5 cut(s) 38, 192, 271, 274, 277
Ple19I CGATCG 1 cut(s) 216
PluTI GGCGCC 2 cut(s) 299, 432
Psp6I CCWGG 2 cut(s) 148, 249
PspGI CCWGG 2 cut(s) 148, 249
PspN4I GGNNCC 3 cut(s) 128, 297, 430
PsuI RGATCY 1 cut(s) 126
PvuI CGATCG 1 cut(s) 216
RsaI GTAC 4 cut(s) 143, 179, 246, 326
RsaNI GTAC 4 cut(s) 142, 178, 245, 325
SatI GCNGC 5 cut(s) 37, 191, 270, 273, 276
Sau3AI GATC 3 cut(s) 126, 213, 411
ScrFI CCNGG 3 cut(s) 87, 150, 251
SduI GDGCHC 1 cut(s) 441
SfoI GGCGCC 2 cut(s) 297, 430
SsiI CCGC 9 cut(s) 25, 36, 76, 90, 96, 270, 273, 276, 444
SspDI GGCGCC 2 cut(s) 295, 428
StyD4I CCNGG 3 cut(s) 85, 148, 249
StyI CCWWGG 1 cut(s) 279
TaaI ACNGT 1 cut(s) 357
TaiI ACGT 2 cut(s) 45, 312
TaqI TCGA 3 cut(s) 135, 225, 306
TauI GCSGC 4 cut(s) 39, 272, 275, 278
TseFI GTSAC 2 cut(s) 310, 357
TseI GCWGC 1 cut(s) 190
Tsp45I GTSAC 2 cut(s) 310, 357
ZraI GACGTC 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.