FvH4_3g20453

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003682
Physical Location & Seq
Forward (+)
13485740 .. 13486555
816 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g20453.t1

Sequence Viewer

Length: 816 bp
ATGATTACTGATGCTAGCTTCTCCTATGGTATTCTTTTTGAAGAAAATTTGTTTGCTAGAGAAAAAAATGGACTAGTGCAAGAGAATACTGAAATGGGTGCAATGAAACAACACTGTTTGTCTCAGGTCTCCTCTACTTTACATCTATATCATGAAGAATTTGGAAGAGCTTTTGCACATTTCCTGTTTGGTGTGAGTAAGCCCTATAATGATAGTTTCTTGCCCTCTGAAGTTGGAGGCACTTGGCTTCTTGATGATAGGATTGAGCACATAAATAGGGATGTCGACACTCTGTTTGACAACAGATCAACTAGAGAAATTAAACTTGATAGTTTCCTAGTTGCTACTGGTTTGGCTTCTGCTATTGATTCAATCGGTATAGCCCCTCTTGGTAGTTCTGACATGGTTTCTAAGAGGTCATATAAGATGGAGATTGTTACTTCATTGTCGTTGATGGAAATGGTAATTTCTAAAGTGTTGAGCATAGAAACTACTGAGAAAGATCAATCACTTATATGTCGATGGCTTATTTGTTTATTCTTTCTCTGTGATTGCAAAGAGAGCATTCATACACTCGCTGGTACTAGGAATGCCTGGTTGACTGGAGATCTTCTGTGTTTACCTCTTGCTGATGACTTAGAGAAAACTAGACAGAACAGGCTACTTAGAAAATTTGTTACTGTTATGGCTGTCAGGAAGGATACAGAGTTTGTGGCTTTGGGAAACAGAAATGGAGATATATGTGTGGTTAAAGTCAAGCAATGGAGCATGAATTGGCTGCGCTCAGACATCACATTTCATCTCCTGGTGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

272

Amino Acids

30.76

Weight (kDa)

5.68

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 285
AcsI RAATTY 3 cut(s) 46, 158, 671
AcuI CTGAAG 1 cut(s) 249
AfaI GTAC 1 cut(s) 583
AgsI TTSAA 2 cut(s) 41, 372
AhlI ACTAGT 1 cut(s) 73
AjnI CCWGG 2 cut(s) 593, 804
AluBI AGCT 2 cut(s) 18, 170
AluI AGCT 2 cut(s) 18, 170
Alw21I GWGCWC 1 cut(s) 270
Alw26I GTCTC 2 cut(s) 126, 133
ApeKI GCWGC 1 cut(s) 778
ApoI RAATTY 3 cut(s) 46, 158, 671
ArsI GACNNNNNNTTYG 2 cut(s) 278, 310
AspLEI GCGC 1 cut(s) 783
AsuNHI GCTAGC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 270
BbvI GCAGC 1 cut(s) 765
BccI CCATC 3 cut(s) 421, 448, 516
BciT130I CCWGG 2 cut(s) 595, 806
BciVI GTATCC 1 cut(s) 694
BcoDI GTCTC 2 cut(s) 126, 133
BcuI ACTAGT 1 cut(s) 73
BfaI CTAG 7 cut(s) 15, 57, 74, 312, 338, 585, 648
BfuI GTATCC 1 cut(s) 694
BglII AGATCT 1 cut(s) 607
BisI GCNGC 1 cut(s) 779
BlsI GCNGC 1 cut(s) 780
Bme1390I CCNGG 2 cut(s) 595, 806
BmrFI CCNGG 2 cut(s) 595, 806
BmtI GCTAGC 1 cut(s) 18
BpmI CTGGAG 1 cut(s) 624
BsaI GGTCTC 1 cut(s) 133
Bse1I ACTGG 2 cut(s) 352, 607
Bse3DI GCAATG 2 cut(s) 108, 767
BseBI CCWGG 2 cut(s) 595, 806
BseGI GGATG 1 cut(s) 286
BseMI GCAATG 2 cut(s) 108, 767
BseMII CTCAG 3 cut(s) 137, 486, 798
BseNI ACTGG 2 cut(s) 352, 607
BseRI GAGGAG 1 cut(s) 121
BseXI GCAGC 1 cut(s) 765
BsiHKAI GWGCWC 1 cut(s) 270
BsmAI GTCTC 2 cut(s) 126, 133
BsmI GAATGC 2 cut(s) 564, 595
Bso31I GGTCTC 1 cut(s) 133
Bsp1286I GDGCHC 1 cut(s) 270
Bsp143I GATC 3 cut(s) 305, 502, 607
BspCNI CTCAG 3 cut(s) 136, 487, 797
BspHI TCATGA 1 cut(s) 151
BspOI GCTAGC 1 cut(s) 18
BspQI GCTCTTC 1 cut(s) 160
BspTNI GGTCTC 1 cut(s) 133
BsrDI GCAATG 2 cut(s) 108, 767
BsrI ACTGG 2 cut(s) 352, 607
BssMI GATC 3 cut(s) 305, 502, 607
Bst2UI CCWGG 2 cut(s) 595, 806
Bst4CI ACNGT 2 cut(s) 116, 682
Bst6I CTCTTC 1 cut(s) 160
BstC8I GCNNGC 1 cut(s) 16
BstDEI CTNAG 6 cut(s) 123, 411, 495, 637, 665, 784
BstF5I GGATG 1 cut(s) 286
BstHHI GCGC 1 cut(s) 783
BstKTI GATC 3 cut(s) 308, 505, 610
BstMAI GTCTC 2 cut(s) 126, 133
BstMBI GATC 3 cut(s) 305, 502, 607
BstMWI GCNNNNNNNGC 1 cut(s) 561
BstNI CCWGG 2 cut(s) 595, 806
BstSCI CCNGG 2 cut(s) 593, 804
BstV1I GCAGC 1 cut(s) 765
BstX2I RGATCY 1 cut(s) 607
BstYI RGATCY 1 cut(s) 607
BsuI GTATCC 1 cut(s) 694
BtsCI GGATG 1 cut(s) 286
BtsIMutI CAGTG 1 cut(s) 112
Cac8I GCNNGC 1 cut(s) 16
CciI TCATGA 1 cut(s) 151
CfoI GCGC 1 cut(s) 783
Csp6I GTAC 1 cut(s) 582
CviAII CATG 3 cut(s) 152, 403, 769
CviQI GTAC 1 cut(s) 582
DdeI CTNAG 6 cut(s) 123, 411, 495, 637, 665, 784
DpnI GATC 3 cut(s) 307, 504, 609
DpnII GATC 3 cut(s) 305, 502, 607
Eam1104I CTCTTC 1 cut(s) 160
EarI CTCTTC 1 cut(s) 160
Eco31I GGTCTC 1 cut(s) 133
Eco57I CTGAAG 1 cut(s) 249
EcoRII CCWGG 2 cut(s) 593, 804
FaeI CATG 3 cut(s) 155, 406, 772
FatI CATG 3 cut(s) 151, 402, 768
FblI GTMKAC 1 cut(s) 285
Fnu4HI GCNGC 1 cut(s) 779
FokI GGATG 1 cut(s) 293
Fsp4HI GCNGC 1 cut(s) 779
FspBI CTAG 7 cut(s) 15, 57, 74, 312, 338, 585, 648
GlaI GCGC 1 cut(s) 782
GluI GCNGC 1 cut(s) 779
GsuI CTGGAG 1 cut(s) 624
HhaI GCGC 1 cut(s) 783
Hin1II CATG 3 cut(s) 155, 406, 772
Hin6I GCGC 1 cut(s) 781
HinP1I GCGC 1 cut(s) 781
HincII GTYRAC 2 cut(s) 286, 600
HindII GTYRAC 2 cut(s) 286, 600
HinfI GANTC 1 cut(s) 368
Hpy166II GTNNAC 3 cut(s) 286, 600, 620
Hpy188I TCNGA 3 cut(s) 229, 400, 787
Hpy188III TCNNGA 3 cut(s) 152, 251, 694
Hpy8I GTNNAC 3 cut(s) 286, 600, 620
HpyAV CCTTC 1 cut(s) 691
HpyCH4III ACNGT 2 cut(s) 116, 682
HpyCH4V TGCA 4 cut(s) 79, 101, 176, 555
HpyF10VI GCNNNNNNNGC 1 cut(s) 561
HpyF3I CTNAG 6 cut(s) 123, 411, 495, 637, 665, 784
Hsp92II CATG 3 cut(s) 155, 406, 772
HspAI GCGC 1 cut(s) 781
Kzo9I GATC 3 cut(s) 305, 502, 607
LguI GCTCTTC 1 cut(s) 160
LmnI GCTCC 1 cut(s) 765
Lsp1109I GCAGC 1 cut(s) 765
MaeI CTAG 7 cut(s) 15, 57, 74, 312, 338, 585, 648
MaeIII GTNAC 2 cut(s) 436, 676
MalI GATC 3 cut(s) 307, 504, 609
MboI GATC 3 cut(s) 305, 502, 607
MboII GAAGA 4 cut(s) 53, 167, 177, 602
MflI RGATCY 1 cut(s) 607
MhlI GDGCHC 1 cut(s) 270
MluCI AATT 6 cut(s) 46, 158, 318, 465, 671, 772
MmeI TCCRAC 1 cut(s) 214
MnlI CCTC 6 cut(s) 142, 230, 235, 396, 408, 633
MseI TTAA 2 cut(s) 321, 750
MslI CAYNNNNRTG 1 cut(s) 514
MspR9I CCNGG 2 cut(s) 595, 806
Mva1269I GAATGC 2 cut(s) 564, 595
MvaI CCWGG 2 cut(s) 595, 806
MwoI GCNNNNNNNGC 1 cut(s) 561
NdeII GATC 3 cut(s) 305, 502, 607
NheI GCTAGC 1 cut(s) 14
NlaIII CATG 3 cut(s) 155, 406, 772
PagI TCATGA 1 cut(s) 151
PciSI GCTCTTC 1 cut(s) 160
PctI GAATGC 2 cut(s) 564, 595
PfeI GAWTC 1 cut(s) 368
PkrI GCNGC 1 cut(s) 780
Psp6I CCWGG 2 cut(s) 593, 804
PspGI CCWGG 2 cut(s) 593, 804
PsuI RGATCY 1 cut(s) 607
RsaI GTAC 1 cut(s) 583
RsaNI GTAC 1 cut(s) 582
RseI CAYNNNNRTG 1 cut(s) 514
SalI GTCGAC 1 cut(s) 284
SapI GCTCTTC 1 cut(s) 160
SaqAI TTAA 2 cut(s) 321, 750
SatI GCNGC 1 cut(s) 779
Sau3AI GATC 3 cut(s) 305, 502, 607
ScrFI CCNGG 2 cut(s) 595, 806
SduI GDGCHC 1 cut(s) 270
SetI ASST 5 cut(s) 20, 129, 172, 419, 625
SmiMI CAYNNNNRTG 1 cut(s) 514
SpeI ACTAGT 1 cut(s) 73
Sse9I AATT 6 cut(s) 46, 158, 318, 465, 671, 772
SspMI CTAG 7 cut(s) 15, 57, 74, 312, 338, 585, 648
StyD4I CCNGG 2 cut(s) 593, 804
TaaI ACNGT 2 cut(s) 116, 682
TaqI TCGA 2 cut(s) 285, 520
TasI AATT 6 cut(s) 46, 158, 318, 465, 671, 772
TfiI GAWTC 1 cut(s) 368
Tru1I TTAA 2 cut(s) 321, 750
Tru9I TTAA 2 cut(s) 321, 750
TscAI CASTG 1 cut(s) 119
TseI GCWGC 1 cut(s) 778
TspDTI ATGAA 6 cut(s) 119, 168, 432, 557, 785, 788
TspRI CASTG 1 cut(s) 119
XapI RAATTY 3 cut(s) 46, 158, 671
XmiI GTMKAC 1 cut(s) 285
XspI CTAG 7 cut(s) 15, 57, 74, 312, 338, 585, 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.