RchiOBHm_Chr4g0393431

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003682
Physical Location & Seq
Forward (+)
8908460 .. 8913905
5446 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36603

Sequence Viewer

Length: 2940 bp
ATGCCAGTTGGGTTTGTCACCGTTCCTGTTGGGATTGTCGGGCCTCTCATTTTTGATGGAAGAGAGTTGTCAGGCCCTATGGCCACCACTGAAGGTTGCCATATGGAAGACCTCGCTAAGAGAGCAGCTGAGTTCAAGTTCTTCATGAAAGACCCTGACAATTATGAAACCAGTTTCCATCTTGAAAAGGCAAAAAAGGGGCCAAAAACACATGAAATTGGGCACCAAGCATCCATTATGTCGGTTAGTGGCATTGAGAATGCCAGAGTAGAGGACCCTACATCTATTCTTGGAGTTAAGAATTCATCAAAGATTCCCTCCCTAGCCATTGACGCAGACAAAGAAGCCTGTAACCATCATCCCTTGGAGAAAAGTTGTGATGACAGTGGAGGATTGAAAACCTTGAATATTCAATTTGAGGTAATTGCTTCTCCATTTGATAAATGTCACTTTTTGAGAGGTGTATCTACTTTGCCTCTTGCAACATGGCTTGAGAAAATTCAAAGGGATGCAGATATGATTTTTTATGGAAATTGGGAGGGGCTTAAAGCCAATGTGGTTGTTGGAACTTCAGTAGTTGAGTTTTGGGACAAGAAGTTAAACATGCATTTCTTTATCAAGTGTAAAGAGCCAGATAAGTACTATATTAGAAATGCAATTTTCAGTCTAGAAATTTCGATTTCATTTGTGACTTTGAAATTGCTCGCATTTAACTCTTGTATGAGTGGGAACATATCTATGGATAACGAAAGGGTCCGGGCATTTGATGATGGTACCATTGGTCATGGTCATCAAGTGGAGAATTGGCTTTCCTTGTCTCTGAGACCAACAAGTTCTCTGATTAGTGCTCTTGAACCCTCTATTTTAATGGTAGTTGGTGGAACTAGCACTAATTATGATCAGGAACAGGTGCCATTGGGTTTCTTGCCTTTGGAACGAATTTTAATGGTAGTTGGTGGAACTAGCACTAATTATGATCAGGGAGTGATGCCTCCTAATTCAACTCTGTATTATGAGGTCGAGCTTTTATCTTTTGTCAAAGACAAGGAACCATGGGACATGAATACTTTAGAGAAAATGGAGGTTGCTGTGTCATTGCTGGAGCAGGATATCAAAACGGGACCATTAGATCAATCTGGTTTAAATTTTGTCAGTGAGATTATTTGTAAATCCAAAGCTCTAATGTTTGAGAGGATGTTGTTTCATGCTACAAGGGGAAATTTGTTTTTCAACCAAACACCAGCAGATGAACATATCATGGATCCTTTATCTAGTGAAATGGTTGAGCAGATAGTTTTTTTTGTCTTCTTCTCAGGGTTGCAGGCAAAAGCAAAGATTCTGAAAATTTGTGAGGCCTTTGGTACAAATTGCTGCCCTGTTCCTGAAGATATAAATAAGCGGAGGCAAATAACTAAAGACGCTTCATCACATCTTGCTGAATTGGAAGCAACTTGGGATGCAGGGCTTCACCACAGAAATAAAGTCATTATTCTCATTATGCTCTACATGAGGCCTCCTGATGATTTCAAGGTTGATGCTAGCAGTGGTTATATTCAAATAGGTGTCCCTCAATCTATTATTAAAGATGCCCAATATGTAGAAATTAGTGCTAGTGATCTCATCAAATGGGACAAGGCCAAAATGGGGTACTACGTTGGCATTGATATGGCTAAAGGATTATTCCTTGGAAATAGCGTGTACTGGATACAATTTGATGAAGAATGGATCGTCCTCCTTCATGTCTTTAAATCACGGGAAGAAGAGTATGGTATGGAGTTCATTTTTGTGAAGGACTCTTATGAACTTATGCACGAATATATTTTGGAGAAGTTCAAATTATATTGGCTATTGCAATATCTATTGCTTCCAGCAGGAAAACAGTCTTTGAGTGTTATCCTTGCCACTAAGTTGATGACTTTGAAATACAAGAAAGATGCACAGGATGCCAATTGCCATGTGGCAATTTTCCATGTTGGTTTTGTGTTGTTATTGCATATGCAACTCGTAGGGATGTGTTTGCCACCTCAAACAGTGGAGTTTCCTCAACATTACTTGACAAAGCAAGAGATAGCCAAATTTGGGATTTGGCTTCCTACAACACTGTCACGTTTGCAGACAAAGGTGATTTTACTGGAAACCACTTATACATGCGCATATTTCCAACCTAAGCAACACAAATGGTCTGCTGCAATTTCTGTTAGGTTAATCTCAAGTGGTCTACATATTACTGATCATAAGCAGATGCTTGAGATGTGTTTGAGTGTTACTAAGTCTACCTTGGATGCTCTTTATATAATGCAGAAGTCGGGTATCACACAAAATTATCAGAGTTTTCTTCAGTTGTATTTGCCTTATGTTTGTGTGGGAAAAGCATTTATAATTCTGCAACATGAGTATCATATTGATCCAGAAGATATCAAGGCGATTATTTCAAGTGCAATTATTCAATTGCATTTAAATGAAGCTACTGAGTTATACAAGGGAATGGACAATCTTCTTAGAGCTTTTGGGATATATATAGTCCTTAATCATTTGGTCTACCATCTTTGCTCAAATGGTCTACAAGTTTTAAATGAGCAATCTGTTGATGTTGTATCCTTTGTGAATTTCCATGATATAATTGGGGATCATTGGCTCCCTGCTCATGATGAAAGATCCATTTGCCAAGGTACAAAGAGAATCAGTAGAAGGTCGCTCGCATTGCTTACCCTTCATCACTGCAATAATAAAATGGTCCAACGATTCAGTTACCTGCGGATAATTCTGAAGAAAGTCATTTTGGCAGAACTGCAAGTTTGTAAGTCTAACTTGGAAAAATTGTCTGATAGTTCTTTTCTTCTGTGGTTATTCAAGTTACTAGAGCCCAAGATCTCATTTGAACGTCCACAACCACGAGTGTACCATGTTGGCAGTAGAAAAACATTGAGGATTAGTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005798 GO:0005802 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006885 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0012505 GO:0012506 GO:0012510 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030641 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0030665 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032588 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042470 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046961 GO:0048770 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051452 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098791 GO:0098796 GO:0098805 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

979

Amino Acids

111.29

Weight (kDa)

6.31

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 376 - 499 8.9e-24 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2378
Acc16I TGCGCA 1 cut(s) 2152
Acc36I ACCTGC 1 cut(s) 2760
Acc65I GGTACC 1 cut(s) 773
AccB1I GGYRCC 3 cut(s) 222, 773, 910
AccI GTMKAC 4 cut(s) 2218, 2273, 2538, 2560
AciI CCGC 2 cut(s) 1399, 2755
AclWI GGATC 6 cut(s) 1256, 1269, 1733, 2399, 2634, 2649
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 9 cut(s) 301, 498, 672, 939, 1144, 1219, 1344, 2075, 2605
AcuI CTGAAG 5 cut(s) 111, 555, 1404, 2321, 2786
AfaI GTAC 7 cut(s) 641, 775, 1363, 1649, 1700, 2671, 2900
AfiI CCNNNNNNNGG 2 cut(s) 1644, 2079
AjuI GAANNNNNNNTTGG 2 cut(s) 2762, 2794
AluBI AGCT 5 cut(s) 128, 1024, 1178, 2465, 2504
AluI AGCT 5 cut(s) 128, 1024, 1178, 2465, 2504
Alw21I GWGCWC 1 cut(s) 850
Alw26I GTCTC 2 cut(s) 817, 822
AlwI GGATC 6 cut(s) 1256, 1269, 1733, 2399, 2634, 2649
AoxI GGCC 7 cut(s) 41, 73, 81, 200, 1353, 1511, 1635
ApeKI GCWGC 3 cut(s) 125, 1371, 2186
ApoI RAATTY 9 cut(s) 301, 498, 672, 939, 1144, 1219, 1344, 2075, 2605
Asp718I GGTACC 1 cut(s) 773
AspLEI GCGC 1 cut(s) 2153
AspS9I GGNCC 7 cut(s) 41, 74, 200, 274, 754, 1121, 2734
AsuC2I CCSGG 1 cut(s) 758
AsuHPI GGTGA 3 cut(s) 10, 1460, 2134
AsuNHI GCTAGC 1 cut(s) 1538
AvaII GGWCC 4 cut(s) 274, 754, 1121, 2734
BaeGI GKGCMC 1 cut(s) 225
BaeI ACNNNNGTAYC 4 cut(s) 2378, 2411, 2882, 2915
BalI TGGCCA 1 cut(s) 83
BamHI GGATCC 1 cut(s) 1261
BanI GGYRCC 3 cut(s) 222, 773, 910
BanII GRGCYC 1 cut(s) 2865
BauI CACGAG 1 cut(s) 2892
BbsI GAAGAC 2 cut(s) 114, 1297
Bbv12I GWGCWC 1 cut(s) 850
BbvI GCAGC 3 cut(s) 137, 1358, 2173
BccI CCATC 5 cut(s) 50, 186, 363, 764, 2550
BciVI GTATCC 2 cut(s) 1698, 2605
BclI TGATCA 3 cut(s) 898, 976, 2230
BcnI CCSGG 1 cut(s) 758
BcoDI GTCTC 2 cut(s) 817, 822
BfaI CTAG 8 cut(s) 323, 668, 885, 963, 1272, 1539, 1611, 2858
BfuAI ACCTGC 1 cut(s) 2760
BfuI GTATCC 2 cut(s) 1698, 2605
BglII AGATCT 1 cut(s) 2868
BisI GCNGC 3 cut(s) 126, 1372, 2187
BlsI GCNGC 3 cut(s) 127, 1373, 2188
BmcAI AGTACT 1 cut(s) 641
Bme1390I CCNGG 1 cut(s) 758
Bme18I GGWCC 4 cut(s) 274, 754, 1121, 2734
BmgT120I GGNCC 7 cut(s) 41, 74, 200, 274, 754, 1121, 2734
BmrFI CCNGG 1 cut(s) 758
BmtI GCTAGC 1 cut(s) 1542
BpiI GAAGAC 2 cut(s) 114, 1297
BpmI CTGGAG 1 cut(s) 1121
Bpu10I CCTNAGC 1 cut(s) 2166
BpuEI CTTGAG 3 cut(s) 512, 2194, 2267
BpuMI CCSGG 1 cut(s) 758
BsaI GGTCTC 1 cut(s) 817
BsaJI CCNNGG 5 cut(s) 363, 1052, 1684, 2277, 2665
Bsc4I CCNNNNNNNGG 2 cut(s) 1644, 2079
Bse1I ACTGG 4 cut(s) 5, 171, 1706, 2136
Bse3DI GCAATG 2 cut(s) 1094, 2699
BseDI CCNNGG 5 cut(s) 363, 1052, 1684, 2277, 2665
BseGI GGATG 8 cut(s) 230, 358, 514, 1200, 1462, 1948, 2016, 2287
BseLI CCNNNNNNNGG 2 cut(s) 1644, 2079
BseMI GCAATG 2 cut(s) 1094, 2699
BseMII CTCAG 4 cut(s) 120, 812, 1326, 2460
BseNI ACTGG 4 cut(s) 5, 171, 1706, 2136
BseSI GKGCMC 1 cut(s) 225
BseXI GCAGC 3 cut(s) 137, 1358, 2173
BshFI GGCC 7 cut(s) 43, 75, 83, 202, 1355, 1513, 1637
BshNI GGYRCC 3 cut(s) 222, 773, 910
BsiHKAI GWGCWC 1 cut(s) 850
BsiSI CCGG 1 cut(s) 757
BslFI GGGAC 5 cut(s) 602, 1070, 1134, 1550, 1643
BslI CCNNNNNNNGG 2 cut(s) 1644, 2079
BsmAI GTCTC 2 cut(s) 817, 822
BsmFI GGGAC 5 cut(s) 602, 1070, 1134, 1550, 1643
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 7 cut(s) 43, 75, 83, 202, 1355, 1513, 1637
Bso31I GGTCTC 1 cut(s) 817
Bsp1286I GDGCHC 3 cut(s) 225, 850, 2865
Bsp19I CCATGG 1 cut(s) 1052
BspACI CCGC 2 cut(s) 1399, 2755
BspANI GGCC 7 cut(s) 43, 75, 83, 202, 1355, 1513, 1637
BspCNI CTCAG 4 cut(s) 121, 813, 1325, 2461
BspHI TCATGA 3 cut(s) 144, 2644, 2936
BspMI ACCTGC 1 cut(s) 2760
BspOI GCTAGC 1 cut(s) 1542
BspPI GGATC 6 cut(s) 1256, 1269, 1733, 2399, 2634, 2649
BspT107I GGYRCC 3 cut(s) 222, 773, 910
BspTNI GGTCTC 1 cut(s) 817
BsrDI GCAATG 2 cut(s) 1094, 2699
BsrI ACTGG 4 cut(s) 5, 171, 1706, 2136
BssECI CCNNGG 5 cut(s) 363, 1052, 1684, 2277, 2665
BssSI CACGAG 1 cut(s) 2892
BssT1I CCWWGG 5 cut(s) 363, 1052, 1684, 2277, 2665
Bst2BI CACGAG 1 cut(s) 2892
Bst4CI ACNGT 5 cut(s) 22, 386, 1881, 2032, 2103
Bst6I CTCTTC 2 cut(s) 55, 1755
BstAPI GCANNNNNTGC 1 cut(s) 1943
BstC8I GCNNGC 4 cut(s) 705, 1323, 1540, 2697
BstDEI CTNAG 9 cut(s) 117, 129, 821, 1312, 1905, 2166, 2268, 2469, 2498
BstDSI CCRYGG 1 cut(s) 1052
BstF5I GGATG 8 cut(s) 230, 358, 514, 1200, 1462, 1948, 2016, 2287
BstHHI GCGC 1 cut(s) 2153
BstMAI GTCTC 2 cut(s) 817, 822
BstMWI GCNNNNNNNGC 4 cut(s) 122, 332, 1943, 2701
BstNSI RCATGY 2 cut(s) 607, 2151
BstSCI CCNGG 1 cut(s) 756
BstSLI GKGCMC 1 cut(s) 225
BstV1I GCAGC 3 cut(s) 137, 1358, 2173
BstV2I GAAGAC 2 cut(s) 114, 1297
BstX2I RGATCY 3 cut(s) 1261, 2654, 2868
BstYI RGATCY 3 cut(s) 1261, 2654, 2868
BsuI GTATCC 2 cut(s) 1698, 2605
BsuRI GGCC 7 cut(s) 43, 75, 83, 202, 1355, 1513, 1637
BtgI CCRYGG 1 cut(s) 1052
BtsCI GGATG 8 cut(s) 230, 358, 514, 1200, 1462, 1948, 2016, 2287
BtsI GCAGTG 2 cut(s) 1549, 2716
BtsIMutI CAGTG 7 cut(s) 87, 391, 1159, 1549, 2037, 2099, 2716
BveI ACCTGC 1 cut(s) 2760
Cac8I GCNNGC 4 cut(s) 705, 1323, 1540, 2697
CciI TCATGA 3 cut(s) 144, 2644, 2936
CfoI GCGC 1 cut(s) 2153
Cfr13I GGNCC 7 cut(s) 41, 74, 200, 274, 754, 1121, 2734
CseI GACGC 2 cut(s) 341, 1427
Csp6I GTAC 7 cut(s) 640, 774, 1362, 1648, 1699, 2670, 2899
CviQI GTAC 7 cut(s) 640, 774, 1362, 1648, 1699, 2670, 2899
DdeI CTNAG 9 cut(s) 117, 129, 821, 1312, 1905, 2166, 2268, 2469, 2498
DraI TTTAAA 4 cut(s) 1143, 1747, 2457, 2571
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 2 cut(s) 55, 1755
EarI CTCTTC 2 cut(s) 55, 1755
Eco130I CCWWGG 5 cut(s) 363, 1052, 1684, 2277, 2665
Eco147I AGGCCT 2 cut(s) 1355, 1513
Eco24I GRGCYC 1 cut(s) 2865
Eco31I GGTCTC 1 cut(s) 817
Eco32I GATATC 2 cut(s) 1111, 2416
Eco47I GGWCC 4 cut(s) 274, 754, 1121, 2734
Eco57I CTGAAG 5 cut(s) 111, 555, 1404, 2321, 2786
EcoO109I RGGNCCY 2 cut(s) 74, 274
EcoRI GAATTC 1 cut(s) 301
EcoRV GATATC 2 cut(s) 1111, 2416
EcoT14I CCWWGG 5 cut(s) 363, 1052, 1684, 2277, 2665
EcoT22I ATGCAT 1 cut(s) 609
EcoT38I GRGCYC 1 cut(s) 2865
ErhI CCWWGG 5 cut(s) 363, 1052, 1684, 2277, 2665
FalI AAGNNNNNCTT 4 cut(s) 2261, 2293, 2792, 2824
FaqI GGGAC 5 cut(s) 602, 1070, 1134, 1550, 1643
FauNDI CATATG 2 cut(s) 102, 1995
FbaI TGATCA 3 cut(s) 898, 976, 2230
FblI GTMKAC 4 cut(s) 2218, 2273, 2538, 2560
Fnu4HI GCNGC 3 cut(s) 126, 1372, 2187
FokI GGATG 8 cut(s) 217, 345, 521, 1207, 1469, 1955, 2023, 2294
FriOI GRGCYC 1 cut(s) 2865
Fsp4HI GCNGC 3 cut(s) 126, 1372, 2187
FspAI RTGCGCAY 1 cut(s) 2152
FspBI CTAG 8 cut(s) 323, 668, 885, 963, 1272, 1539, 1611, 2858
FspI TGCGCA 1 cut(s) 2152
GlaI GCGC 1 cut(s) 2152
GluI GCNGC 3 cut(s) 126, 1372, 2187
GsuI CTGGAG 1 cut(s) 1121
HaeIII GGCC 7 cut(s) 43, 75, 83, 202, 1355, 1513, 1637
HapII CCGG 1 cut(s) 757
HgaI GACGC 2 cut(s) 341, 1427
HhaI GCGC 1 cut(s) 2153
Hin6I GCGC 1 cut(s) 2151
HinP1I GCGC 1 cut(s) 2151
HinfI GANTC 5 cut(s) 313, 1336, 1793, 2679, 2742
HpaII CCGG 1 cut(s) 757
HphI GGTGA 3 cut(s) 10, 1460, 2134
Hpy166II GTNNAC 7 cut(s) 1699, 2219, 2274, 2539, 2561, 2885, 2899
Hpy188I TCNGA 6 cut(s) 822, 840, 1341, 2328, 2766, 2824
Hpy8I GTNNAC 7 cut(s) 1699, 2219, 2274, 2539, 2561, 2885, 2899
HpyAV CCTTC 5 cut(s) 86, 1745, 1783, 2682, 2720
HpyCH4III ACNGT 5 cut(s) 22, 386, 1881, 2032, 2103
HpyCH4IV ACGT 3 cut(s) 1653, 2107, 2881
HpyF10VI GCNNNNNNNGC 4 cut(s) 122, 332, 1943, 2701
HpyF3I CTNAG 9 cut(s) 117, 129, 821, 1312, 1905, 2166, 2268, 2469, 2498
HpySE526I ACGT 3 cut(s) 1653, 2107, 2881
HspAI GCGC 1 cut(s) 2151
KpnI GGTACC 1 cut(s) 777
Ksp22I TGATCA 3 cut(s) 898, 976, 2230
LmnI GCTCC 2 cut(s) 1102, 2640
Lsp1109I GCAGC 3 cut(s) 137, 1358, 2173
MaeI CTAG 8 cut(s) 323, 668, 885, 963, 1272, 1539, 1611, 2858
MaeII ACGT 3 cut(s) 1653, 2107, 2881
MaeIII GTNAC 8 cut(s) 16, 350, 446, 688, 2103, 2263, 2747, 2853
MfeI CAATTG 2 cut(s) 1948, 2447
MflI RGATCY 3 cut(s) 1261, 2654, 2868
MhlI GDGCHC 3 cut(s) 225, 850, 2865
MlsI TGGCCA 1 cut(s) 83
MluNI TGGCCA 1 cut(s) 83
MlyI GAGTC 1 cut(s) 1787
MmeI TCCRAC 3 cut(s) 544, 2185, 2761
Mox20I TGGCCA 1 cut(s) 83
Mph1103I ATGCAT 1 cut(s) 609
MscI TGGCCA 1 cut(s) 83
MslI CAYNNNNRTG 4 cut(s) 737, 1664, 1784, 2457
Msp20I TGGCCA 1 cut(s) 83
MspA1I CMGCKG 1 cut(s) 128
MspI CCGG 1 cut(s) 757
MspR9I CCNGG 1 cut(s) 758
MunI CAATTG 2 cut(s) 1948, 2447
Mva1269I GAATGC 1 cut(s) 265
MwoI GCNNNNNNNGC 4 cut(s) 122, 332, 1943, 2701
NciI CCSGG 1 cut(s) 758
NcoI CCATGG 1 cut(s) 1052
NdeI CATATG 2 cut(s) 102, 1995
NheI GCTAGC 1 cut(s) 1538
NmuCI GTSAC 4 cut(s) 16, 446, 688, 2103
NsbI TGCGCA 1 cut(s) 2152
NsiI ATGCAT 1 cut(s) 609
NspI RCATGY 2 cut(s) 607, 2151
PagI TCATGA 3 cut(s) 144, 2644, 2936
PceI AGGCCT 2 cut(s) 1355, 1513
PctI GAATGC 1 cut(s) 265
PfeI GAWTC 4 cut(s) 313, 1336, 2679, 2742
PkrI GCNGC 3 cut(s) 127, 1373, 2188
PleI GAGTC 1 cut(s) 1787
PpsI GAGTC 1 cut(s) 1787
PpuMI RGGWCCY 1 cut(s) 274
PsiI TTATAA 1 cut(s) 2378
Psp5II RGGWCCY 1 cut(s) 274
PspPI GGNCC 7 cut(s) 41, 74, 200, 274, 754, 1121, 2734
PspPPI RGGWCCY 1 cut(s) 274
PsuI RGATCY 3 cut(s) 1261, 2654, 2868
PvuII CAGCTG 1 cut(s) 128
RsaI GTAC 7 cut(s) 641, 775, 1363, 1649, 1700, 2671, 2900
RsaNI GTAC 7 cut(s) 640, 774, 1362, 1648, 1699, 2670, 2899
RseI CAYNNNNRTG 4 cut(s) 737, 1664, 1784, 2457
SatI GCNGC 3 cut(s) 126, 1372, 2187
Sau96I GGNCC 7 cut(s) 41, 74, 200, 274, 754, 1121, 2734
ScaI AGTACT 1 cut(s) 641
SchI GAGTC 1 cut(s) 1787
ScrFI CCNGG 1 cut(s) 758
SduI GDGCHC 3 cut(s) 225, 850, 2865
SinI GGWCC 4 cut(s) 274, 754, 1121, 2734
SmiI ATTTAAAT 1 cut(s) 2457
SmiMI CAYNNNNRTG 4 cut(s) 737, 1664, 1784, 2457
SmlI CTYRAG 3 cut(s) 491, 2209, 2246
SmoI CTYRAG 3 cut(s) 491, 2209, 2246
SseBI AGGCCT 2 cut(s) 1355, 1513
SsiI CCGC 2 cut(s) 1399, 2755
SspI AATATT 1 cut(s) 409
SspMI CTAG 8 cut(s) 323, 668, 885, 963, 1272, 1539, 1611, 2858
StuI AGGCCT 2 cut(s) 1355, 1513
StyD4I CCNGG 1 cut(s) 756
StyI CCWWGG 5 cut(s) 363, 1052, 1684, 2277, 2665
SwaI ATTTAAAT 1 cut(s) 2457
TaaI ACNGT 5 cut(s) 22, 386, 1881, 2032, 2103
TaiI ACGT 3 cut(s) 1656, 2110, 2884
TaqI TCGA 2 cut(s) 677, 1020
TatI WGTACW 2 cut(s) 639, 1698
TfiI GAWTC 4 cut(s) 313, 1336, 2679, 2742
TscAI CASTG 7 cut(s) 94, 391, 1159, 1549, 2037, 2106, 2723
TseFI GTSAC 4 cut(s) 16, 446, 688, 2103
TseI GCWGC 3 cut(s) 125, 1371, 2186
Tsp45I GTSAC 4 cut(s) 16, 446, 688, 2103
TspRI CASTG 7 cut(s) 94, 391, 1159, 1549, 2037, 2106, 2723
VpaK11BI GGWCC 4 cut(s) 274, 754, 1121, 2734
XapI RAATTY 9 cut(s) 301, 498, 672, 939, 1144, 1219, 1344, 2075, 2605
XbaI TCTAGA 1 cut(s) 667
XceI RCATGY 2 cut(s) 607, 2151
XcmI CCANNNNNNNNNTGG 2 cut(s) 1954, 2618
XmiI GTMKAC 4 cut(s) 2218, 2273, 2538, 2560
XspI CTAG 8 cut(s) 323, 668, 885, 963, 1272, 1539, 1611, 2858
ZrmI AGTACT 1 cut(s) 641
Zsp2I ATGCAT 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.