Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0003682

Physical Location & Seq

Genomic Coordinates Forward (+)

61078162 .. 61081988

Gene Structure 3827 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00267930.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3429 bp

ATGACCCATTCCGTTTATTCACTACCACAAACCCCTAATCACCTCTTTTACACCACTTCTCAGCCTCTGCAACATCCGAGAAATTTCCATTCACCATGTCAACATCCTCCAACTCCGGGTGAGGTCTATCACCATTACCCACCTACCCAGCCTTATTGCATCCCCACAAACCAGTTTATTCCTCCACCTTCCACTCATGAGCAACCACGTCCTATTTCAGATCTAAAGCAGGTGGAAACTGACCTTCAGCTTCGTCCAATTTCAATGGAACCTGACCGGGTACATGATTGGGAGGTCCCAATTGTTGATTCAGGCTACTCCTCTTTCACACCAGAGCCTACGGTCAAGCTTAAGGGGCTGAGAAACCACCGTCACCACCCCGAGAGTTCGACGGATGCCTTCATGTCTGAATTCAGGGAGGAGCTACGGCGGTTAAGGGCTTCGAAGAAAACTCCGACAGGCCAAGTTGATAATCCATTGAAGTTTGGCTCTCTGCCATCGTCAACACATGAAATTATTCCGGCTACTATTAAGGCTTCTTCGTCAGTCGTATCGTCTTCTAATGGTACTCTGATAATAACTTCTCTTAATACTGGGTTGAATTTATCTCACCATGAAGAGGTTGTGAGTGGTGCTAATACTAGCTGTGTGACTTCTTCTGAAACTATGGTGAAACTCTCTAGATGTACTGCTCCCCCTCTTTGTAATTTGGCCCTTGACATTGCTACTGTTTTGCGCTTAGTTGTGACTGAAGAAGATCATCTTCTGTTGGATATGATTTCATTGGTTGGGCAAGGGGAAGCCGATGATAAACCATCCTTGAGTCTATTTGAGCGAATAATTAATGCCCTTTCTATATCTTGTAAATCGAGTACTCTTCCTGTTGATTCCTTTACTTTTGTCTTCCCTATAATGGAGCAAACTCTTTTAAGCTCCGAGAAGACCGAGCTTCATGATAATGTGCTTCAGATTATTTATATGCACATGAACCCCATATTACCTCTCCATAGGCTTCAAGTGATTTCAGTTCCTTATTATGTTCTTGGTATTGTACAAGCTTACCAATACTGTATTGGATCTGTGTTGAATGAGCTGTGTTTTGGTCTGCAGCTAGATGGAGTTGCACCGATTTTATACAGGGTTTATGCAAAAGTTGTTCACATTAGAATGGCCTCTCTAACTGCTGTCAAATGCATTCCTACTGTTGCCATTCGGTTTCTTATTCAAAAGGTTGAGGCTGCTACAAGTATTTGGATTGCTTTACATGATCCAGAAAAGTCAGTTACCAATGCTATAGAGGATTTCTGGGATTGTTATGGAAATGACTTTGGGACTGACTATTTAGGCCTTTTCAAAGCATGTTCCCATGTCCATTATACGGTCCGTTATCCTGCTGCAGAGGTATTAGCCGCTGCTTTGGACAAATTTCCTGATTCTATACAGGAATCCCTTTCTACTTTACTTCTCATGTATGTCTGTGATGCTAGTTTGTCTGAGAACAATGTCAATACGGGTTGGCTCAAGGTTGACAGTGAGATGGTGATGTGTTCTGCTGATGCACAATCTGATGCGAAGTTATGGCCATGGTTCATTCTTTGTTTACAGCTTTATCATTGGGAAACAAAGAAATCTATTCTCGTCTATAGGGAAGCATTCAACCAAATGGGTGAAATGAGTCAAACCACATTATCTCATAATGGGCCTACACTTGCTGGTAAGGATAATCTGATAGGAATTGTAGCAATTATTGACTGGTTTCGGTGTGCTTTATCAGAATTTGGCACTGTGGCTACTCTAAATGCATGCAAGGATGGAAAATATGTTGCTCTGGGAAGCAAAGATGTAGATACATTTGTGGTTGTAATTAAGAAAATGGAACATGAATTTATTGAGCTCCGAAATCACTTTTTGTCTCAAGGAGTAGTGAACTTGCTTGAAGAGGTATCTGAACTTGCTTTTGCAAAATTTGGTCCCAGTGTTATTTTATTGACTGGTTTACAAGGAATTGGGAAGACAACAATATTCACTACATTGAAGATGGCATTTTTGTTGTGCCAGGTTGCACTAACACGCATGGGAAAGTTACAAGATGAAAGATTTTTATTCTCTGGTAGCTTTAGTTATGATAATTATGCATGTTTTGATATTGTCATGGAAAAACAATTACCAAATGCAGCTTGTCATTCAATCTTGTTTAGGCTCATATTGGGAGCTCTTAGACAAGAATCATCAGAAGCCCTAAGGAGATGGCAATATGCACTGCTTCTTAGCTCTTTTCAGTATTGTCAACACATGCTTGATCTAGACATCCCATCAACAGTTCTGCAATTTTTGTTGCTTGATGAGCAAGAGGTTGAAGATCTGGATCTCCAGAAAATTAGATGTGAAATTGGAGAGATGATGTCACTCTACGTTTTGGATGCAATCATATGCGTAGATCATGATGGGGTACATTCTCAAAACTCAATGCAGCAGCAAGCACAAACTCCGGAGGCTGAGCTTGCTTTGCTATTGAAAATAAAATATCTCTTTGCATCCTTTATTTCCCGTGATGAAGACTATTATGCATGTGTTGAAGGGAGAGAAATTATCTCCAATCTTAGTATCACAGCTGCCTCAAAATTTCTATTAAATTTGTTGGTCCAGCCTGAGCATGAACACCGCAAATGGTCTGCTGCAATTTCTGTTGGATTAATCTCAAGTGGTCTATATATTACTGATCATAAGCAAAAGTTTGAGAAGTGTTTGGGAGTTGTTAACTCTACCTTCAAACGAAATCAGAGTTTCCTTCAGTTGTATTTGCCTTATGTTAGTGTGGGAAAAGCATTTACAAGTCTGCAAAATGAGTATCAAATTGATCAAGTAGATGTCAGGGCTGTTATTGCAATTGTAATTATTCAATTGCATTCAAATGAAGCTACTGAGTTATGCAAGGGGATGGACAAACTTCTTCAAGCTTTTGGGATATATATAGCCTTTGCTTATTTGGCCTACCATCTTTGCTCAGTTGGTCCACAACTGTTAATTGAGCAATTCATTGATGTTGCATTCGTTGGGAATTTTTATGATACAAATGTCGATCAATGGCTCCCTACTCATGATGAAAGATTCATTTGCAAAGGTACGAAGAGAATCAGCAGAAGGTTACTCACATTGCTTCAAAACCCCTTTACCAATGTCAGAATGGGTATGATGACACCATCCTGCACCTCCAATGATAAGCTTGGGGTGCATGAGGCCACAAAGACGGACCAACTTGCTGATAAAGACAATGTCTCATTTCATCAATGGGGTATCACATTTGTCATCTTCTACTTCGAGTTTGTCTATCATTTCTTCTTGTCTCAGTGCTGCTGGTATAACATTCTTGTCAAGATGCTCCACATGGTTGATTTTTTTCAAGATGCTCCACATGCTCCCAGATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009682 GO:0016020 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0050896 GO:0051704 GO:0051707 GO:0098542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CLASP_N HEAT HEAT_2

Protein Analysis

1142

Amino Acids

128.94

Weight (kDa)

5.7

Isoelectric Point (pI)

42.49

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003682)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g12421 FvH4_3g20453 FvH4_3g20454
malus_domestica	MD16G1253500.v1.1
rosa_chinensis	RchiOBHm_Chr3g0479611 RchiOBHm_Chr4g0393431 RchiOBHm_Chr4g0403131 RchiOBHm_Chr4g0403161 RchiOBHm_Chr4g0419441 RchiOBHm_Chr5g0007271 RchiOBHm_Chr5g0056401
rosa_multiflora	Rmu_sc0000577.1_g000014 Rmu_sc0001218.1_g000016 Rmu_sc0001787.1_g000002 Rmu_sc0001877.1_g000005 Rmu_sc0002396.1_g000007 Rmu_sc0002522.1_g000025 Rmu_sc0002699.1_g000023 Rmu_sc0002822.1_g000015 Rmu_sc0002822.1_g000017 Rmu_sc0003347.1_g000019 Rmu_sc0004174.1_g000005 Rmu_sc0004874.1_g000013 Rmu_sc0009422.1_g000002 Rmu_sc0017595.1_g000001 Rmu_sc0032285.1_g000001
rosa_roxburghii	Rroxscaffold_1G00033040 Rroxscaffold_2G00106480 Rroxscaffold_3G00267930 Rroxscaffold_4G00326640 Rroxscaffold_5G00382820 Rroxscaffold_6G00403290 Rroxscaffold_7G00191420
rosa_samantha	Rh2AG247000 Rh6CG057200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	220
Acc36I	ACCTGC	1 cut(s)	220
AccIII	TCCGGA	1 cut(s)	2488
AciI	CCGC	3 cut(s)	430, 1410, 2662
AclWI	GGATC	3 cut(s)	1084, 1262, 2373
AcoI	YGGCCR	1 cut(s)	1580
AcuI	CTGAAG	4 cut(s)	230, 771, 950, 2774
AfaI	GTAC	7 cut(s)	282, 568, 688, 874, 1053, 2451, 3124
AfiI	CCNNNNNNNGG	4 cut(s)	116, 619, 913, 1378
AflII	CTTAAG	1 cut(s)	350
AjiI	CACGTC	1 cut(s)	209
AjnI	CCWGG	1 cut(s)	2055
AjuI	GAANNNNNNNTTGG	2 cut(s)	292, 324
Alw21I	GWGCWC	2 cut(s)	1896, 2215
Alw26I	GTCTC	3 cut(s)	1917, 3280, 3348
AlwI	GGATC	3 cut(s)	1084, 1262, 2373
AlwNI	CAGNNNCTG	1 cut(s)	67
Ama87I	CYCGRG	1 cut(s)	380
Aor13HI	TCCGGA	1 cut(s)	2488
AoxI	GGCC	8 cut(s)	460, 711, 1170, 1345, 1580, 1700, 2988, 3237
AseI	ATTAAT	2 cut(s)	843, 2693
Asp700I	GAANNNNTTC	1 cut(s)	516
AspLEI	GCGC	1 cut(s)	738
AspS9I	GGNCC	8 cut(s)	295, 712, 1381, 1700, 1970, 2641, 3011, 3250
AsuC2I	CCSGG	2 cut(s)	117, 278
AsuHPI	GGTGA	9 cut(s)	32, 84, 122, 131, 365, 602, 682, 1552, 1679
AsuII	TTCGAA	1 cut(s)	443
AvaI	CYCGRG	1 cut(s)	380
AvaII	GGWCC	6 cut(s)	295, 1381, 1970, 2641, 3011, 3250
AxyI	CCTNAGG	1 cut(s)	2240
BaeI	ACNNNNGTAYC	4 cut(s)	1839, 1872, 3060, 3093
BalI	TGGCCA	1 cut(s)	1582
BanII	GRGCYC	2 cut(s)	1896, 2215
BbsI	GAAGAC	5 cut(s)	549, 895, 947, 2018, 2562
Bbv12I	GWGCWC	2 cut(s)	1896, 2215
BceAI	ACGGC	1 cut(s)	443
BciT130I	CCWGG	1 cut(s)	2057
BclI	TGATCA	2 cut(s)	2719, 2857
BcnI	CCSGG	2 cut(s)	117, 278
BcoDI	GTCTC	3 cut(s)	1917, 3280, 3348
BfaI	CTAG	5 cut(s)	642, 681, 1112, 1485, 2303
BfmI	CTRYAG	4 cut(s)	1106, 1293, 1395, 1642
BfrI	CTTAAG	1 cut(s)	350
BfuAI	ACCTGC	1 cut(s)	220
BglII	AGATCT	2 cut(s)	220, 2359
BlpI	GCTNAGC	1 cut(s)	2496
BmcAI	AGTACT	1 cut(s)	874
Bme1390I	CCNGG	3 cut(s)	117, 278, 2057
Bme18I	GGWCC	6 cut(s)	295, 1381, 1970, 2641, 3011, 3250
BmeT110I	CYCGRG	1 cut(s)	380
BmgBI	CACGTC	1 cut(s)	209
BmgT120I	GGNCC	8 cut(s)	295, 712, 1381, 1700, 1970, 2641, 3011, 3250
BmiI	GGNNCC	4 cut(s)	270, 297, 1972, 3089
BmrFI	CCNGG	3 cut(s)	117, 278, 2057
BmrI	ACTGGG	2 cut(s)	603, 1968
BmsI	GCATC	9 cut(s)	168, 385, 1471, 1546, 1558, 2410, 2543, 3366, 3394
BmuI	ACTGGG	2 cut(s)	603, 1968
BpiI	GAAGAC	5 cut(s)	549, 895, 947, 2018, 2562
BpmI	CTGGAG	1 cut(s)	2354
Bpu10I	CCTNAGC	1 cut(s)	2649
Bpu1102I	GCTNAGC	1 cut(s)	2496
Bpu14I	TTCGAA	1 cut(s)	443
BpuEI	CTTGAG	4 cut(s)	841, 1505, 1899, 2683
BpuMI	CCSGG	2 cut(s)	117, 278
BsaBI	GATNNNNATC	1 cut(s)	2364
BsaJI	CCNNGG	1 cut(s)	1583
BsaWI	WCCGGW	1 cut(s)	2488
Bsc4I	CCNNNNNNNGG	4 cut(s)	116, 619, 913, 1378
Bse1I	ACTGG	5 cut(s)	172, 598, 1757, 1974, 1996
Bse21I	CCTNAGG	1 cut(s)	2240
Bse3DI	GCAATG	2 cut(s)	720, 3152
Bse8I	GATNNNNATC	1 cut(s)	2364
BseAI	TCCGGA	1 cut(s)	2488
BseBI	CCWGG	1 cut(s)	2057
BseDI	CCNNGG	1 cut(s)	1583
BseJI	GATNNNNATC	1 cut(s)	2364
BseLI	CCNNNNNNNGG	4 cut(s)	116, 619, 913, 1378
BseMI	GCAATG	2 cut(s)	720, 3152
BseMII	CTCAG	8 cut(s)	74, 350, 1485, 2487, 2640, 2913, 3018, 3359
BseNI	ACTGG	5 cut(s)	172, 598, 1757, 1974, 1996
BseRI	GAGGAG	2 cut(s)	310, 434
BseYI	CCCAGC	1 cut(s)	147
BsgI	GTGCAG	1 cut(s)	3190
BshFI	GGCC	8 cut(s)	462, 713, 1172, 1347, 1582, 1702, 2990, 3239
BsiHKAI	GWGCWC	2 cut(s)	1896, 2215
BsiHKCI	CYCGRG	1 cut(s)	380
BsiSI	CCGG	4 cut(s)	116, 277, 521, 2489
BslFI	GGGAC	3 cut(s)	281, 1345, 1956
BslI	CCNNNNNNNGG	4 cut(s)	116, 619, 913, 1378
BsmAI	GTCTC	3 cut(s)	1917, 3280, 3348
BsmFI	GGGAC	3 cut(s)	281, 1345, 1956
BsmI	GAATGC	4 cut(s)	1194, 1652, 2905, 3047
BsnI	GGCC	8 cut(s)	462, 713, 1172, 1347, 1582, 1702, 2990, 3239
BsoBI	CYCGRG	1 cut(s)	380
Bsp119I	TTCGAA	1 cut(s)	443
Bsp1286I	GDGCHC	2 cut(s)	1896, 2215
Bsp13I	TCCGGA	1 cut(s)	2488
Bsp1407I	TGTACA	1 cut(s)	1051
Bsp1720I	GCTNAGC	1 cut(s)	2496
Bsp19I	CCATGG	1 cut(s)	1583
BspACI	CCGC	3 cut(s)	430, 1410, 2662
BspANI	GGCC	8 cut(s)	462, 713, 1172, 1347, 1582, 1702, 2990, 3239
BspCNI	CTCAG	8 cut(s)	73, 351, 1486, 2488, 2641, 2914, 3017, 3358
BspEI	TCCGGA	1 cut(s)	2488
BspHI	TCATGA	4 cut(s)	196, 952, 2440, 3097
BspLI	GGNNCC	4 cut(s)	270, 297, 1972, 3089
BspMAI	CTGCAG	2 cut(s)	1110, 1399
BspMI	ACCTGC	1 cut(s)	220
BspPI	GGATC	3 cut(s)	1084, 1262, 2373
BspT104I	TTCGAA	1 cut(s)	443
BspTI	CTTAAG	1 cut(s)	350
BsrDI	GCAATG	2 cut(s)	720, 3152
BsrGI	TGTACA	1 cut(s)	1051
BsrI	ACTGG	5 cut(s)	172, 598, 1757, 1974, 1996
BssECI	CCNNGG	1 cut(s)	1583
BssT1I	CCWWGG	1 cut(s)	1583
Bst2UI	CCWGG	1 cut(s)	2057
Bst6I	CTCTTC	4 cut(s)	612, 882, 1932, 3122
BstAFI	CTTAAG	1 cut(s)	350
BstAUI	TGTACA	1 cut(s)	1051
BstBI	TTCGAA	1 cut(s)	443
BstC8I	GCNNGC	3 cut(s)	1804, 2478, 2502
BstDSI	CCRYGG	1 cut(s)	1583
BstHHI	GCGC	1 cut(s)	738
BstMAI	GTCTC	3 cut(s)	1917, 3280, 3348
BstMWI	GCNNNNNNNGC	8 cut(s)	355, 2344, 2501, 2506, 2882, 2987, 3229, 3413
BstNI	CCWGG	1 cut(s)	2057
BstNSI	RCATGY	6 cut(s)	1362, 1806, 2139, 2296, 2571, 3416
BstSCI	CCNGG	3 cut(s)	115, 276, 2055
BstSFI	CTRYAG	4 cut(s)	1106, 1293, 1395, 1642
BstV2I	GAAGAC	5 cut(s)	549, 895, 947, 2018, 2562
BstX2I	RGATCY	4 cut(s)	220, 1076, 2359, 2365
BstYI	RGATCY	4 cut(s)	220, 1076, 2359, 2365
Bsu36I	CCTNAGG	1 cut(s)	2240
BsuRI	GGCC	8 cut(s)	462, 713, 1172, 1347, 1582, 1702, 2990, 3239
BtgI	CCRYGG	1 cut(s)	1583
BtrI	CACGTC	1 cut(s)	209
BtsI	GCAGTG	1 cut(s)	2258
BtsIMutI	CAGTG	5 cut(s)	1537, 1782, 1981, 2258, 3353
BveI	ACCTGC	1 cut(s)	220
Cac8I	GCNNGC	3 cut(s)	1804, 2478, 2502
CaiI	CAGNNNCTG	1 cut(s)	67
CciI	TCATGA	4 cut(s)	196, 952, 2440, 3097
CfoI	GCGC	1 cut(s)	738
Cfr13I	GGNCC	8 cut(s)	295, 712, 1381, 1700, 1970, 2641, 3011, 3250
CpoI	CGGWCCG	1 cut(s)	1381
Csp6I	GTAC	7 cut(s)	281, 567, 687, 873, 1052, 2450, 3123
CspI	CGGWCCG	1 cut(s)	1381
CviQI	GTAC	7 cut(s)	281, 567, 687, 873, 1052, 2450, 3123
EaeI	YGGCCR	1 cut(s)	1580
Eam1104I	CTCTTC	4 cut(s)	612, 882, 1932, 3122
EarI	CTCTTC	4 cut(s)	612, 882, 1932, 3122
Ecl136II	GAGCTC	2 cut(s)	1894, 2213
Eco130I	CCWWGG	1 cut(s)	1583
Eco147I	AGGCCT	1 cut(s)	1347
Eco24I	GRGCYC	2 cut(s)	1896, 2215
Eco47I	GGWCC	6 cut(s)	295, 1381, 1970, 2641, 3011, 3250
Eco53kI	GAGCTC	2 cut(s)	1894, 2213
Eco57I	CTGAAG	4 cut(s)	230, 771, 950, 2774
Eco81I	CCTNAGG	1 cut(s)	2240
Eco88I	CYCGRG	1 cut(s)	380
EcoICRI	GAGCTC	2 cut(s)	1894, 2213
EcoO109I	RGGNCCY	1 cut(s)	295
EcoRI	GAATTC	1 cut(s)	410
EcoRII	CCWGG	1 cut(s)	2055
EcoT14I	CCWWGG	1 cut(s)	1583
EcoT22I	ATGCAT	4 cut(s)	1196, 1804, 2137, 2569
EcoT38I	GRGCYC	2 cut(s)	1896, 2215
ErhI	CCWWGG	1 cut(s)	1583
FalI	AAGNNNNNCTT	2 cut(s)	747, 779
FaqI	GGGAC	3 cut(s)	281, 1345, 1956
FauNDI	CATATG	1 cut(s)	2429
FbaI	TGATCA	2 cut(s)	2719, 2857
FriOI	GRGCYC	2 cut(s)	1896, 2215
FspBI	CTAG	5 cut(s)	642, 681, 1112, 1485, 2303
GlaI	GCGC	1 cut(s)	737
GsaI	CCCAGC	1 cut(s)	151
GsuI	CTGGAG	1 cut(s)	2354
HaeIII	GGCC	8 cut(s)	462, 713, 1172, 1347, 1582, 1702, 2990, 3239
HapII	CCGG	4 cut(s)	116, 277, 521, 2489
HhaI	GCGC	1 cut(s)	738
Hin6I	GCGC	1 cut(s)	736
HinP1I	GCGC	1 cut(s)	736
HincII	GTYRAC	5 cut(s)	101, 504, 1528, 2288, 2758
HindII	GTYRAC	5 cut(s)	101, 504, 1528, 2288, 2758
HindIII	AAGCTT	4 cut(s)	347, 1056, 2955, 3221
HinfI	GANTC	9 cut(s)	308, 823, 887, 1433, 1445, 1675, 2225, 3108, 3132
HpaI	GTTAAC	1 cut(s)	2758
HpaII	CCGG	4 cut(s)	116, 277, 521, 2489
HphI	GGTGA	9 cut(s)	32, 84, 122, 131, 365, 602, 682, 1552, 1679
Hpy99I	CGWCG	1 cut(s)	394
HpyAV	CCTTC	7 cut(s)	198, 254, 409, 2570, 2776, 2798, 3135
HpyCH4IV	ACGT	2 cut(s)	208, 2412
HpyF10VI	GCNNNNNNNGC	8 cut(s)	355, 2344, 2501, 2506, 2882, 2987, 3229, 3413
HpySE526I	ACGT	2 cut(s)	208, 2412
HspAI	GCGC	1 cut(s)	736
Kpn2I	TCCGGA	1 cut(s)	2488
Ksp22I	TGATCA	2 cut(s)	2719, 2857
KspAI	GTTAAC	1 cut(s)	2758
LweI	GCATC	9 cut(s)	168, 385, 1471, 1546, 1558, 2410, 2543, 3366, 3394
MaeI	CTAG	5 cut(s)	642, 681, 1112, 1485, 2303
MaeII	ACGT	2 cut(s)	208, 2412
MaeIII	GTNAC	7 cut(s)	371, 649, 745, 1282, 2082, 2403, 3144
MfeI	CAATTG	3 cut(s)	300, 2886, 2900
MflI	RGATCY	4 cut(s)	220, 1076, 2359, 2365
MhlI	GDGCHC	2 cut(s)	1896, 2215
MlsI	TGGCCA	1 cut(s)	1582
MluNI	TGGCCA	1 cut(s)	1582
MlyI	GAGTC	2 cut(s)	832, 1684
MmeI	TCCRAC	4 cut(s)	134, 479, 750, 2668
Mox20I	TGGCCA	1 cut(s)	1582
Mph1103I	ATGCAT	4 cut(s)	1196, 1804, 2137, 2569
MroI	TCCGGA	1 cut(s)	2488
MroXI	GAANNNNTTC	1 cut(s)	516
MscI	TGGCCA	1 cut(s)	1582
MslI	CAYNNNNRTG	3 cut(s)	957, 1166, 2910
Msp20I	TGGCCA	1 cut(s)	1582
MspA1I	CMGCKG	2 cut(s)	1412, 2612
MspCI	CTTAAG	1 cut(s)	350
MspI	CCGG	4 cut(s)	116, 277, 521, 2489
MspR9I	CCNGG	3 cut(s)	117, 278, 2057
MunI	CAATTG	3 cut(s)	300, 2886, 2900
Mva1269I	GAATGC	4 cut(s)	1194, 1652, 2905, 3047
MvaI	CCWGG	1 cut(s)	2057
MwoI	GCNNNNNNNGC	8 cut(s)	355, 2344, 2501, 2506, 2882, 2987, 3229, 3413
NciI	CCSGG	2 cut(s)	117, 278
NcoI	CCATGG	1 cut(s)	1583
NdeI	CATATG	1 cut(s)	2429
NlaIV	GGNNCC	4 cut(s)	270, 297, 1972, 3089
NmuCI	GTSAC	4 cut(s)	371, 649, 745, 2403
NsiI	ATGCAT	4 cut(s)	1196, 1804, 2137, 2569
NspI	RCATGY	6 cut(s)	1362, 1806, 2139, 2296, 2571, 3416
NspV	TTCGAA	1 cut(s)	443
PaeI	GCATGC	1 cut(s)	1806
PagI	TCATGA	4 cut(s)	196, 952, 2440, 3097
PaqCI	CACCTGC	1 cut(s)	220
PceI	AGGCCT	1 cut(s)	1347
PctI	GAATGC	4 cut(s)	1194, 1652, 2905, 3047
PdmI	GAANNNNTTC	1 cut(s)	516
PfeI	GAWTC	7 cut(s)	308, 887, 1433, 1445, 2225, 3108, 3132
PflFI	GACNNNGTC	1 cut(s)	3272
PleI	GAGTC	2 cut(s)	831, 1683
PpsI	GAGTC	2 cut(s)	831, 1683
PpuMI	RGGWCCY	1 cut(s)	295
PshBI	ATTAAT	2 cut(s)	843, 2693
Psp124BI	GAGCTC	2 cut(s)	1896, 2215
Psp5II	RGGWCCY	1 cut(s)	295
Psp6I	CCWGG	1 cut(s)	2055
PspFI	CCCAGC	1 cut(s)	147
PspGI	CCWGG	1 cut(s)	2055
PspN4I	GGNNCC	4 cut(s)	270, 297, 1972, 3089
PspPI	GGNCC	8 cut(s)	295, 712, 1381, 1700, 1970, 2641, 3011, 3250
PspPPI	RGGWCCY	1 cut(s)	295
PstI	CTGCAG	2 cut(s)	1110, 1399
PstNI	CAGNNNCTG	1 cut(s)	67
PsuI	RGATCY	4 cut(s)	220, 1076, 2359, 2365
PsyI	GACNNNGTC	1 cut(s)	3272
PvuII	CAGCTG	1 cut(s)	2612
RsaI	GTAC	7 cut(s)	282, 568, 688, 874, 1053, 2451, 3124
RsaNI	GTAC	7 cut(s)	281, 567, 687, 873, 1052, 2450, 3123
RseI	CAYNNNNRTG	3 cut(s)	957, 1166, 2910
Rsr2I	CGGWCCG	1 cut(s)	1381
RsrII	CGGWCCG	1 cut(s)	1381
SacI	GAGCTC	2 cut(s)	1896, 2215
Sau96I	GGNCC	8 cut(s)	295, 712, 1381, 1700, 1970, 2641, 3011, 3250
ScaI	AGTACT	1 cut(s)	874
SchI	GAGTC	2 cut(s)	832, 1684
ScrFI	CCNGG	3 cut(s)	117, 278, 2057
SduI	GDGCHC	2 cut(s)	1896, 2215
SfaNI	GCATC	9 cut(s)	168, 385, 1471, 1546, 1558, 2410, 2543, 3366, 3394
SfcI	CTRYAG	4 cut(s)	1106, 1293, 1395, 1642
SfuI	TTCGAA	1 cut(s)	443
SinI	GGWCC	6 cut(s)	295, 1381, 1970, 2641, 3011, 3250
SmiMI	CAYNNNNRTG	3 cut(s)	957, 1166, 2910
SmlI	CTYRAG	5 cut(s)	350, 820, 1520, 1914, 2698
SmoI	CTYRAG	5 cut(s)	350, 820, 1520, 1914, 2698
SphI	GCATGC	1 cut(s)	1806
SseBI	AGGCCT	1 cut(s)	1347
SsiI	CCGC	3 cut(s)	430, 1410, 2662
SspI	AATATT	1 cut(s)	2022
SspMI	CTAG	5 cut(s)	642, 681, 1112, 1485, 2303
SstI	GAGCTC	2 cut(s)	1896, 2215
StuI	AGGCCT	1 cut(s)	1347
StyD4I	CCNGG	3 cut(s)	115, 276, 2055
StyI	CCWWGG	1 cut(s)	1583
TaiI	ACGT	2 cut(s)	211, 2415
TaqI	TCGA	5 cut(s)	389, 443, 869, 3078, 3318
TaqII	GACCGA	1 cut(s)	959
TatI	WGTACW	3 cut(s)	686, 872, 1051
TauI	GCSGC	1 cut(s)	1412
TfiI	GAWTC	7 cut(s)	308, 887, 1433, 1445, 2225, 3108, 3132
TscAI	CASTG	5 cut(s)	1537, 1789, 1981, 2265, 3353
TseFI	GTSAC	4 cut(s)	371, 649, 745, 2403
Tsp45I	GTSAC	4 cut(s)	371, 649, 745, 2403
TspGWI	ACGGA	3 cut(s)	407, 1373, 3263
TspRI	CASTG	5 cut(s)	1537, 1789, 1981, 2265, 3353
Tth111I	GACNNNGTC	1 cut(s)	3272
Vha464I	CTTAAG	1 cut(s)	350
VpaK11BI	GGWCC	6 cut(s)	295, 1381, 1970, 2641, 3011, 3250
VspI	ATTAAT	2 cut(s)	843, 2693
XbaI	TCTAGA	2 cut(s)	680, 2302
XceI	RCATGY	6 cut(s)	1362, 1806, 2139, 2296, 2571, 3416
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1070, 3181
XmnI	GAANNNNTTC	1 cut(s)	516
XspI	CTAG	5 cut(s)	642, 681, 1112, 1485, 2303
ZrmI	AGTACT	1 cut(s)	874
Zsp2I	ATGCAT	4 cut(s)	1196, 1804, 2137, 2569

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.