Rmu_sc0000577.1_g000014

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000577.1
OG0003682
Physical Location & Seq
Reverse (-)
47999 .. 49178
1180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000577.1_g000014.1.cds

Sequence Viewer

Length: 1002 bp
atggataacgaaagggtccgggcatttgatgatggtaccattggtcatggtcatcaagtggagaatcggctttccttgtctctgagaccaacaagttctctgattagtgctcttgaaccctctattttaatggtagttggtggaactagcactaattatgatcaggaacaggtgccattgggtttcttgcctttggaaagaattttaatggtagttggtggaactagcactaattatgatcagagagtgatgcctcctaattcaactctgtattatgaggtcgagcttttatcttttgtcaaagacaaggaaccgtgggacatgaatactctagagaaaatggaggttgttgtgtcattgttggagcaggatatcgaaacgggaccattagatcaatctgggttgcaggcaaaagcaaagattctgaaaatttgtgaggcctttggtacaaattgctgccctgttcctgaagatataaataagcggaggcaaataactaaagaagcttcatcacatcttgctgaattggaagcaacttgggatgcagggattcaccacagaaataaagtcattattctcattatgctctacatgaggcctcctgatgatttcaaggttgatactagcagtggttatattcaaataggtatccctcaatctattattaaagatgcccaatatgtagaaattagtgctagtgatctcatcaaatgggacaaggccaaaatgggttactacgttggcattgatatggctaaaggattattccttggaaatagcgtgtactggatacaattcgatgaagaatggatcgtcctccttcatgtctttaaatcacgggaagaagagtatggtatggagttcatttttgtgaaggactcttacgaacttatgcacgaatatattttggagaagttattggctattgcaatatctattgcttccagcgggaaaacattctttgaggtactcttctgctccaattattttaatgtctgctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005798 GO:0005802 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006885 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0012505 GO:0012506 GO:0012510 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030641 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0030665 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032588 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042470 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046961 GO:0048770 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051452 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098791 GO:0098796 GO:0098805 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

333

Amino Acids

37.82

Weight (kDa)

4.81

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 35
AccB1I GGYRCC 2 cut(s) 35, 172
AciI CCGC 2 cut(s) 484, 948
AclWI GGATC 1 cut(s) 818
AcsI RAATTY 2 cut(s) 201, 429
AcuI CTGAAG 1 cut(s) 489
AfaI GTAC 4 cut(s) 37, 448, 785, 969
AgsI TTSAA 4 cut(s) 116, 264, 613, 641
AluBI AGCT 2 cut(s) 286, 506
AluI AGCT 2 cut(s) 286, 506
Alw21I GWGCWC 1 cut(s) 112
Alw26I GTCTC 2 cut(s) 79, 84
AlwI GGATC 1 cut(s) 818
AoxI GGCC 3 cut(s) 438, 596, 720
ApeKI GCWGC 1 cut(s) 456
ApoI RAATTY 2 cut(s) 201, 429
Asp700I GAANNNNTTC 1 cut(s) 956
Asp718I GGTACC 1 cut(s) 35
AspS9I GGNCC 2 cut(s) 16, 383
AsuC2I CCSGG 1 cut(s) 20
AsuHPI GGTGA 1 cut(s) 545
AvaII GGWCC 2 cut(s) 16, 383
BanI GGYRCC 2 cut(s) 35, 172
BarI GAAGNNNNNNTAC 2 cut(s) 866, 898
Bbv12I GWGCWC 1 cut(s) 112
BbvI GCAGC 1 cut(s) 443
BccI CCATC 1 cut(s) 26
BciVI GTATCC 2 cut(s) 659, 783
BclI TGATCA 2 cut(s) 160, 238
BcnI CCSGG 1 cut(s) 20
BcoDI GTCTC 2 cut(s) 79, 84
BfaI CTAG 6 cut(s) 147, 225, 332, 624, 696, 1000
BfuI GTATCC 2 cut(s) 659, 783
BisI GCNGC 1 cut(s) 457
BlsI GCNGC 1 cut(s) 458
Bme1390I CCNGG 1 cut(s) 20
Bme18I GGWCC 2 cut(s) 16, 383
BmgT120I GGNCC 2 cut(s) 16, 383
BmiI GGNNCC 5 cut(s) 17, 37, 174, 312, 384
BmrFI CCNGG 1 cut(s) 20
BmsI GCATC 3 cut(s) 240, 532, 661
BpuMI CCSGG 1 cut(s) 20
BsaI GGTCTC 1 cut(s) 79
BsaJI CCNNGG 2 cut(s) 314, 769
Bse1I ACTGG 1 cut(s) 791
BseDI CCNNGG 2 cut(s) 314, 769
BseGI GGATG 1 cut(s) 547
BseMII CTCAG 1 cut(s) 74
BseNI ACTGG 1 cut(s) 791
BseXI GCAGC 1 cut(s) 443
BshFI GGCC 3 cut(s) 440, 598, 722
BshNI GGYRCC 2 cut(s) 35, 172
BsiHKAI GWGCWC 1 cut(s) 112
BsiSI CCGG 1 cut(s) 19
BslFI GGGAC 3 cut(s) 332, 396, 728
BsmAI GTCTC 2 cut(s) 79, 84
BsmFI GGGAC 3 cut(s) 332, 396, 728
BsnI GGCC 3 cut(s) 440, 598, 722
Bso31I GGTCTC 1 cut(s) 79
Bsp1286I GDGCHC 1 cut(s) 112
Bsp143I GATC 5 cut(s) 160, 238, 391, 700, 810
BspACI CCGC 2 cut(s) 484, 948
BspANI GGCC 3 cut(s) 440, 598, 722
BspCNI CTCAG 1 cut(s) 75
BspLI GGNNCC 5 cut(s) 17, 37, 174, 312, 384
BspPI GGATC 1 cut(s) 818
BspT107I GGYRCC 2 cut(s) 35, 172
BspTNI GGTCTC 1 cut(s) 79
BsrI ACTGG 1 cut(s) 791
BssECI CCNNGG 2 cut(s) 314, 769
BssMI GATC 5 cut(s) 160, 238, 391, 700, 810
BssT1I CCWWGG 1 cut(s) 769
Bst4CI ACNGT 1 cut(s) 315
Bst6I CTCTTC 2 cut(s) 840, 977
BstC8I GCNNGC 1 cut(s) 408
BstDEI CTNAG 1 cut(s) 83
BstDSI CCRYGG 1 cut(s) 314
BstF5I GGATG 1 cut(s) 547
BstKTI GATC 5 cut(s) 163, 241, 394, 703, 813
BstMAI GTCTC 2 cut(s) 79, 84
BstMBI GATC 5 cut(s) 160, 238, 391, 700, 810
BstSCI CCNGG 1 cut(s) 18
BstV1I GCAGC 1 cut(s) 443
BsuI GTATCC 2 cut(s) 659, 783
BsuRI GGCC 3 cut(s) 440, 598, 722
BtgI CCRYGG 1 cut(s) 314
BtsCI GGATG 1 cut(s) 547
BtsI GCAGTG 1 cut(s) 634
BtsIMutI CAGTG 1 cut(s) 634
Cac8I GCNNGC 1 cut(s) 408
Cfr13I GGNCC 2 cut(s) 16, 383
Csp6I GTAC 4 cut(s) 36, 447, 784, 968
CviAII CATG 4 cut(s) 47, 322, 592, 824
CviJI RGCY 8 cut(s) 70, 286, 440, 506, 598, 722, 755, 923
CviKI_1 RGCY 8 cut(s) 70, 286, 440, 506, 598, 722, 755, 923
CviQI GTAC 4 cut(s) 36, 447, 784, 968
DdeI CTNAG 1 cut(s) 83
DpnI GATC 5 cut(s) 162, 240, 393, 702, 812
DpnII GATC 5 cut(s) 160, 238, 391, 700, 810
DraI TTTAAA 1 cut(s) 832
Eam1104I CTCTTC 2 cut(s) 840, 977
EarI CTCTTC 2 cut(s) 840, 977
Eco130I CCWWGG 1 cut(s) 769
Eco147I AGGCCT 2 cut(s) 440, 598
Eco31I GGTCTC 1 cut(s) 79
Eco32I GATATC 1 cut(s) 373
Eco47I GGWCC 2 cut(s) 16, 383
Eco57I CTGAAG 1 cut(s) 489
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 1 cut(s) 769
ErhI CCWWGG 1 cut(s) 769
FaeI CATG 4 cut(s) 50, 325, 595, 827
FaqI GGGAC 3 cut(s) 332, 396, 728
FatI CATG 4 cut(s) 46, 321, 591, 823
FauI CCCGC 1 cut(s) 941
FbaI TGATCA 2 cut(s) 160, 238
Fnu4HI GCNGC 1 cut(s) 457
FokI GGATG 1 cut(s) 554
Fsp4HI GCNGC 1 cut(s) 457
FspBI CTAG 6 cut(s) 147, 225, 332, 624, 696, 1000
GluI GCNGC 1 cut(s) 457
HaeIII GGCC 3 cut(s) 440, 598, 722
HapII CCGG 1 cut(s) 19
Hin1II CATG 4 cut(s) 50, 325, 595, 827
HindIII AAGCTT 1 cut(s) 504
HinfI GANTC 4 cut(s) 64, 421, 550, 878
HpaII CCGG 1 cut(s) 19
HphI GGTGA 1 cut(s) 545
Hpy166II GTNNAC 1 cut(s) 784
Hpy188I TCNGA 4 cut(s) 84, 102, 243, 426
Hpy188III TCNNGA 5 cut(s) 113, 164, 332, 467, 602
Hpy8I GTNNAC 1 cut(s) 784
HpyAV CCTTC 2 cut(s) 830, 868
HpyCH4III ACNGT 1 cut(s) 315
HpyCH4IV ACGT 1 cut(s) 738
HpyCH4V TGCA 4 cut(s) 406, 545, 895, 929
HpyF3I CTNAG 1 cut(s) 83
HpySE526I ACGT 1 cut(s) 738
Hsp92II CATG 4 cut(s) 50, 325, 595, 827
KpnI GGTACC 1 cut(s) 39
Ksp22I TGATCA 2 cut(s) 160, 238
Kzo9I GATC 5 cut(s) 160, 238, 391, 700, 810
LmnI GCTCC 2 cut(s) 364, 983
Lsp1109I GCAGC 1 cut(s) 443
LweI GCATC 3 cut(s) 240, 532, 661
MaeI CTAG 6 cut(s) 147, 225, 332, 624, 696, 1000
MaeII ACGT 1 cut(s) 738
MaeIII GTNAC 1 cut(s) 731
MalI GATC 5 cut(s) 162, 240, 393, 702, 812
MboI GATC 5 cut(s) 160, 238, 391, 700, 810
MboII GAAGA 5 cut(s) 482, 815, 854, 857, 964
MhlI GDGCHC 1 cut(s) 112
MlyI GAGTC 1 cut(s) 872
MmeI TCCRAC 1 cut(s) 342
MroXI GAANNNNTTC 1 cut(s) 956
MseI TTAA 5 cut(s) 128, 206, 666, 831, 990
MslI CAYNNNNRTG 2 cut(s) 749, 869
MspA1I CMGCKG 1 cut(s) 948
MspI CCGG 1 cut(s) 19
MspR9I CCNGG 1 cut(s) 20
NciI CCSGG 1 cut(s) 20
NdeII GATC 5 cut(s) 160, 238, 391, 700, 810
NlaIII CATG 4 cut(s) 50, 325, 595, 827
NlaIV GGNNCC 5 cut(s) 17, 37, 174, 312, 384
PceI AGGCCT 2 cut(s) 440, 598
PdmI GAANNNNTTC 1 cut(s) 956
PfeI GAWTC 3 cut(s) 64, 421, 550
PkrI GCNGC 1 cut(s) 458
PleI GAGTC 1 cut(s) 872
PpsI GAGTC 1 cut(s) 872
PspN4I GGNNCC 5 cut(s) 17, 37, 174, 312, 384
PspPI GGNCC 2 cut(s) 16, 383
RsaI GTAC 4 cut(s) 37, 448, 785, 969
RsaNI GTAC 4 cut(s) 36, 447, 784, 968
RseI CAYNNNNRTG 2 cut(s) 749, 869
SaqAI TTAA 5 cut(s) 128, 206, 666, 831, 990
SatI GCNGC 1 cut(s) 457
Sau3AI GATC 5 cut(s) 160, 238, 391, 700, 810
Sau96I GGNCC 2 cut(s) 16, 383
SchI GAGTC 1 cut(s) 872
ScrFI CCNGG 1 cut(s) 20
SduI GDGCHC 1 cut(s) 112
SetI ASST 9 cut(s) 174, 282, 288, 348, 508, 618, 649, 741, 969
SfaNI GCATC 3 cut(s) 240, 532, 661
SinI GGWCC 2 cut(s) 16, 383
SmiMI CAYNNNNRTG 2 cut(s) 749, 869
SseBI AGGCCT 2 cut(s) 440, 598
SsiI CCGC 2 cut(s) 484, 948
SspMI CTAG 6 cut(s) 147, 225, 332, 624, 696, 1000
StuI AGGCCT 2 cut(s) 440, 598
StyD4I CCNGG 1 cut(s) 18
StyI CCWWGG 1 cut(s) 769
TaaI ACNGT 1 cut(s) 315
TaiI ACGT 1 cut(s) 741
TaqI TCGA 3 cut(s) 282, 375, 798
TatI WGTACW 1 cut(s) 783
TfiI GAWTC 3 cut(s) 64, 421, 550
Tru1I TTAA 5 cut(s) 128, 206, 666, 831, 990
Tru9I TTAA 5 cut(s) 128, 206, 666, 831, 990
TscAI CASTG 1 cut(s) 634
TseI GCWGC 1 cut(s) 456
TspDTI ATGAA 5 cut(s) 338, 498, 812, 816, 853
TspRI CASTG 1 cut(s) 634
VpaK11BI GGWCC 2 cut(s) 16, 383
XapI RAATTY 2 cut(s) 201, 429
XbaI TCTAGA 1 cut(s) 331
XmnI GAANNNNTTC 1 cut(s) 956
XspI CTAG 6 cut(s) 147, 225, 332, 624, 696, 1000
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.