FvH4_4g10120

Ion channel CASTOR-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0001000
Physical Location & Seq
Forward (+)
12972704 .. 12982137
9434 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g10120.t3

Sequence Viewer

Length: 2700 bp
ATGTCCCTTGAGTCCGACTCAGCGGTCCCTTCCTCGAGCAGAGACTGGTACTTTCCCTCACCGCCCTTCATCCACGCGGCAAAATCCCGAAAATACCACCGAAGATTCTCCACAAACCCCAGACCATCCCCCTCCGATTCTCAGCTGCCATTTGCCGCCGGCGTTTCGTCTCCCAATCCGGCTACGCCGTTTCGTGCCGTTCCTCCGAGAACTTTCAGCCATGAAAGGCCCCGCCGGAGAGTTGACTTCTCCCGCCGGCGAGATAAGCCGCCGCGAACAGATGTCAACCAGCCCGTTTCCGGCGTCCCCGACGGCCTCTCCAATAAACGATCGGAGGCGTCCGGCGACAAGTTCATTAGGTTTGCCGGGCCCAGCTTCAAGCTTCGGTGGAAAATGGCCTTTTCGGTATCGATTTTGATGACAGTTTTCTCATCAGTTGTGTACCAAAATTTCTGTTTACATAGCCAAGTGGATGAGCTGCAGAATTATATTTCCAAGCTTGAATTTAGATTACAAGCCTCCCATTCGTCAGACTCTATGGAGCTCATCAGTTATTCTGAGCCGGAGGGTGATCTTCCCAACAGAAGCTTGAAACGATTAGCTCTTATGGTCTCCCTCATACTTTTGTCAGTCCCTGTTGTAGTTTTCAAGTACATTGACTACGTCTCGAGATCACGATCACCAGAGAACATCTCTGAAGAAGAGTCTCTAAACAAGCAGCTAGCGTACCGTGTTGATTTGTTTTTATCAGTCCACCCGTATTCTAAGCCATTGGCCTTGTTGGTTGCAACACTATTGCTTATTTGTCTCGGGGGGTTAGCACTCTTTGGAGTGAAAGATGATAGCTTAGTGGAGTGTCTTTGGTTATCGTGGACGTACGTAGCTGATTCAGGCAACCATACTGACTCTGAAAAAATTGGCGAAAGGCTTGTTTCAGTTTCCATTAGCTTTGGTGGGATGCTGATATTTGCAATGATGCTTGGACTTGTCTCTGATGCAATTTCGGAGAAGTTTGACTCATTGAGGAAGGGAAGAAGTGAGGTTGTGGAGCAAAACCATACTTTAATCCTTGGTTGGAGCGATAAGTTGGGATCACTGTTGAACCAGATTGCGATAGCCAATGAGAGTTTGGGTGGAGGGATTGTTGTAGTTATGGCTGAGCGAGACAAAGAGGAAATGGAACTTGACATTGGCAAAATGGAGTTTGATTTCAAGGGAACATCTATAATATGCAGAAGTGGGAGCCCTCTTATTCTTGCTGACCTGAAAAAGGTATCTGTCTCGAAGGCACGTGCAATAATTGTCCTTGCTGAAGATGGAAATGCTGATCAGAGTGATGCCCGTGCATTGAGAACTGTTTTAAGCCTGACTGGAGTGAAAGAGGGGCTGAGAGGACATATTGTTGTTGAACTAAGTGATCTTGACAATGAGGTTCTTGTTAAACTTGTTGGTGGAGATCTTGTTGAAACTGTTGTGGCTCATGATGTAATTGGTCGTCTGATGATTCAATGTGCTAGGCAGCCTGGACTTGCACAGATTTGGGAAGATATCCTTGGGTTTGAAAATTGTGAGTTCTATATCAAAAGATGGCCACAGTTGGATGGCATGCATTTTGAGGATGTTCTGATCAGTTTTCCTGATGCTATTCCTTGTGGAGTCAAGGTTGCATCATTGGGTGGTAGAATTATTTTGAACCCGGATGACTCTTATGTTCTACAAGAAGGCGATGAAGTACTTGTCATAGCAGAGGACGACGATACCTATGCTCCTGCAGAATTGCCTATGTTCAACAAAGTGAAAATAAACGAAAAATTGAGTGGAACTGAAGCTGTGGAAAAGGTCAAAGAGGCATCTTTCATTCAAATTTCCCGAACAGCAAGAAAGCCACAGAAGATTCTACTTTGTGGATGGAGGAGGGACATAGATGATATGCTTGTGGTGTTGGATGCCTTTCTAGCCCCTGGTTCCGAGCTCTGGATGTTCAATGAGGTTGCAGAGAAGGAGAGGAAAAAGAAGCTCATTGATGGCGGTCTTGAAATCAATCGTTTAGTTAATATATCTTTAGTTGACCGTGAAGGAAATGCTGTCATACGGCGTCATTTAGAAAGCCTTCCTTTGCAATCTTTTGATTCGATCTTAATTTTGGCTGATGAGTCTGTGGAAGACTCAGCAATTCAAGCTGATTCAAGATCTCTTGCCACTTTGCTTTTGATTCGCGATATTCAGGCCAAGCGTCTCCCTATGGTAACACATGTTGAAAGAGGAAGCTTCTCACAAAGTTCATGGATAGGGGAAATGCAACAAGCTTCTGATAAGTCGGTTATAATTAGTGAAATCCTGGACCCAAGGACCAAAAATCTATTATCCATGTCAAAGATCAGTGACTATGTGCTATCGAATGAGCTTGTCAGCATGGCCCTCGCTATGGTAGCAGAGGATCGGCAGATAAATGATGTGTTGGAAGAGTTGTTTGCGGAGGAGGGAAACGAATTGCAAATAAGGCAAGGAGATCTTTATCTCCATGAAGATGAGGAGTTGAGCTTCTATGAAGTACTGTTGCGTGCACGACAAAGGCGAGAGGTCATGATTGGTTATCGTTTAGCTGATGCTGAGAGAGCTGTCATCAACCCTCCTGCTAAAACTGAGAGACGAAGGTGGTCAGTGAAGGATGTATTTGTGGTGATTGCAGAGAAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Castor_Poll_mid

Protein Analysis

900

Amino Acids

100.62

Weight (kDa)

5.32

Isoelectric Point (pI)

55.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Castor_Poll_mid PF06241 494 - 591 1e-42 Castor and Pollux, part of voltage-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2324
AasI GACNNNNNNGTC 1 cut(s) 23
AccII CGCG 3 cut(s) 77, 274, 2217
AclWI GGATC 2 cut(s) 1099, 2445
AcoI YGGCCR 1 cut(s) 1589
AcsI RAATTY 3 cut(s) 448, 503, 1863
AcuI CTGAAG 3 cut(s) 717, 1332, 1845
AcvI CACGTG 1 cut(s) 1292
AcyI GRCGYC 3 cut(s) 303, 338, 2095
AfaI GTAC 7 cut(s) 50, 443, 653, 728, 878, 1734, 2553
AfiI CCNNNNNNNGG 4 cut(s) 299, 1270, 1974, 2425
AflIII ACRYGT 1 cut(s) 2251
AjnI CCWGG 3 cut(s) 1522, 1960, 2337
AjuI GAANNNNNNNTTGG 4 cut(s) 1173, 1205, 1536, 1568
Alw21I GWGCWC 3 cut(s) 546, 1974, 2566
Alw44I GTGCAC 1 cut(s) 2562
AlwI GGATC 2 cut(s) 1099, 2445
AlwNI CAGNNNCTG 2 cut(s) 45, 635
Ama87I CYCGRG 3 cut(s) 34, 667, 809
AoxI GGCC 8 cut(s) 227, 313, 368, 396, 774, 1589, 2226, 2415
ApaI GGGCCC 1 cut(s) 372
ApaLI GTGCAC 1 cut(s) 2562
ApeKI GCWGC 4 cut(s) 145, 478, 718, 1519
ApoI RAATTY 3 cut(s) 448, 503, 1863
Asp700I GAANNNNTTC 1 cut(s) 2109
AspS9I GGNCC 7 cut(s) 25, 228, 368, 369, 2341, 2349, 2416
AsuC2I CCSGG 2 cut(s) 367, 1697
AsuHPI GGTGA 4 cut(s) 51, 581, 672, 2692
AsuNHI GCTAGC 1 cut(s) 721
AvaI CYCGRG 3 cut(s) 34, 667, 809
AvaII GGWCC 3 cut(s) 25, 2341, 2349
BaeGI GKGCMC 2 cut(s) 372, 2566
BalI TGGCCA 1 cut(s) 1591
BanII GRGCYC 4 cut(s) 372, 546, 1247, 1974
BbrPI CACGTG 1 cut(s) 1292
BbsI GAAGAC 1 cut(s) 2169
Bbv12I GWGCWC 3 cut(s) 546, 1974, 2566
BbvI GCAGC 4 cut(s) 132, 465, 730, 1531
BccI CCATC 6 cut(s) 133, 1310, 1581, 1595, 1902, 2018
BceAI ACGGC 4 cut(s) 172, 182, 328, 2108
BcgI CGANNNNNNTGC 4 cut(s) 1745, 1779, 2401, 2435
BciT130I CCWGG 3 cut(s) 1524, 1962, 2339
BclI TGATCA 2 cut(s) 1327, 1626
BcnI CCSGG 2 cut(s) 367, 1697
BfaI CTAG 3 cut(s) 722, 1515, 1955
BfmI CTRYAG 2 cut(s) 479, 1770
BglII AGATCT 3 cut(s) 1456, 2189, 2509
BisI GCNGC 8 cut(s) 78, 146, 156, 269, 272, 479, 719, 1520
BlpI GCTNAGC 1 cut(s) 1158
BlsI GCNGC 8 cut(s) 79, 147, 157, 270, 273, 480, 720, 1521
BmcAI AGTACT 2 cut(s) 1734, 2553
Bme1390I CCNGG 5 cut(s) 367, 1524, 1697, 1962, 2339
Bme18I GGWCC 3 cut(s) 25, 2341, 2349
BmeT110I CYCGRG 3 cut(s) 34, 667, 809
BmgT120I GGNCC 7 cut(s) 25, 228, 368, 369, 2341, 2349, 2416
BmiI GGNNCC 6 cut(s) 27, 230, 370, 1244, 1966, 2343
BmrFI CCNGG 5 cut(s) 367, 1524, 1697, 1962, 2339
BmsI GCATC 9 cut(s) 948, 966, 985, 1327, 1630, 1676, 1859, 1936, 2596
BmtI GCTAGC 1 cut(s) 725
BpiI GAAGAC 1 cut(s) 2169
BplI GAGNNNNNCTC 3 cut(s) 34, 677, 709
BpmI CTGGAG 1 cut(s) 1392
Bpu1102I GCTNAGC 1 cut(s) 1158
BpuEI CTTGAG 1 cut(s) 29
BpuMI CCSGG 2 cut(s) 367, 1697
Bsa29I ATCGAT 1 cut(s) 410
BsaAI YACGTR 2 cut(s) 880, 1292
BsaBI GATNNNNATC 2 cut(s) 676, 2514
BsaHI GRCGYC 3 cut(s) 303, 338, 2095
BsaI GGTCTC 1 cut(s) 616
BsaJI CCNNGG 4 cut(s) 1069, 1552, 1960, 2345
Bsc4I CCNNNNNNNGG 4 cut(s) 299, 1270, 1974, 2425
Bse118I RCCGGY 2 cut(s) 158, 255
Bse1I ACTGG 2 cut(s) 50, 1375
Bse3DI GCAATG 1 cut(s) 978
Bse8I GATNNNNATC 2 cut(s) 676, 2514
BseBI CCWGG 3 cut(s) 1524, 1962, 2339
BseCI ATCGAT 1 cut(s) 410
BseDI CCNNGG 4 cut(s) 1069, 1552, 1960, 2345
BseJI GATNNNNATC 2 cut(s) 676, 2514
BseLI CCNNNNNNNGG 4 cut(s) 299, 1270, 1974, 2425
BseMI GCAATG 1 cut(s) 978
BseMII CTCAG 8 cut(s) 33, 155, 549, 1149, 1379, 2181, 2601, 2634
BseNI ACTGG 2 cut(s) 50, 1375
BseRI GAGGAG 3 cut(s) 1927, 2492, 2546
BseSI GKGCMC 2 cut(s) 372, 2566
BseXI GCAGC 4 cut(s) 132, 465, 730, 1531
BseYI CCCAGC 1 cut(s) 371
Bsh1236I CGCG 3 cut(s) 77, 274, 2217
Bsh1285I CGRYCG 1 cut(s) 332
BshFI GGCC 8 cut(s) 229, 315, 370, 398, 776, 1591, 2228, 2417
BshVI ATCGAT 1 cut(s) 410
BsiEI CGRYCG 1 cut(s) 332
BsiHKAI GWGCWC 3 cut(s) 546, 1974, 2566
BsiHKCI CYCGRG 3 cut(s) 34, 667, 809
BsiSI CCGG 9 cut(s) 159, 179, 235, 256, 300, 342, 366, 563, 1697
BsiWI CGTACG 1 cut(s) 876
BslFI GGGAC 4 cut(s) 11, 290, 617, 1931
BslI CCNNNNNNNGG 4 cut(s) 299, 1270, 1974, 2425
BsmBI CGTCTC 4 cut(s) 174, 670, 2240, 2641
BsmFI GGGAC 4 cut(s) 11, 290, 617, 1931
BsnI GGCC 8 cut(s) 229, 315, 370, 398, 776, 1591, 2228, 2417
Bso31I GGTCTC 1 cut(s) 616
BsoBI CYCGRG 3 cut(s) 34, 667, 809
Bsp120I GGGCCC 1 cut(s) 368
Bsp1286I GDGCHC 5 cut(s) 372, 546, 1247, 1974, 2566
Bsp1720I GCTNAGC 1 cut(s) 1158
Bsp68I TCGCGA 1 cut(s) 2217
BspANI GGCC 8 cut(s) 229, 315, 370, 398, 776, 1591, 2228, 2417
BspCNI CTCAG 8 cut(s) 32, 154, 550, 1150, 1380, 2180, 2602, 2635
BspDI ATCGAT 1 cut(s) 410
BspFNI CGCG 3 cut(s) 77, 274, 2217
BspHI TCATGA 2 cut(s) 1480, 2583
BspLI GGNNCC 6 cut(s) 27, 230, 370, 1244, 1966, 2343
BspMAI CTGCAG 2 cut(s) 483, 1774
BspOI GCTAGC 1 cut(s) 725
BspPI GGATC 2 cut(s) 1099, 2445
BspTNI GGTCTC 1 cut(s) 616
BsrDI GCAATG 1 cut(s) 978
BsrFI RCCGGY 2 cut(s) 158, 255
BsrI ACTGG 2 cut(s) 50, 1375
BssAI RCCGGY 2 cut(s) 158, 255
BssECI CCNNGG 4 cut(s) 1069, 1552, 1960, 2345
BssNI GRCGYC 3 cut(s) 303, 338, 2095
BssT1I CCWWGG 3 cut(s) 1069, 1552, 2345
Bst2UI CCWGG 3 cut(s) 1524, 1962, 2339
Bst4CI ACNGT 8 cut(s) 424, 731, 1098, 1357, 1471, 1596, 2072, 2556
Bst6I CTCTTC 2 cut(s) 696, 2457
BstACI GRCGYC 3 cut(s) 303, 338, 2095
BstBAI YACGTR 2 cut(s) 880, 1292
BstC8I GCNNGC 5 cut(s) 160, 257, 723, 1607, 2562
BstENI CCTNNNNNAGG 1 cut(s) 1268
BstFNI CGCG 3 cut(s) 77, 274, 2217
BstMCI CGRYCG 1 cut(s) 332
BstMWI GCNNNNNNNGC 6 cut(s) 265, 1955, 2177, 2429, 2500, 2615
BstNI CCWGG 3 cut(s) 1524, 1962, 2339
BstNSI RCATGY 2 cut(s) 1609, 2255
BstSCI CCNGG 5 cut(s) 365, 1522, 1695, 1960, 2337
BstSFI CTRYAG 2 cut(s) 479, 1770
BstSLI GKGCMC 2 cut(s) 372, 2566
BstSNI TACGTA 1 cut(s) 880
BstUI CGCG 3 cut(s) 77, 274, 2217
BstV1I GCAGC 4 cut(s) 132, 465, 730, 1531
BstV2I GAAGAC 1 cut(s) 2169
BstX2I RGATCY 3 cut(s) 1456, 2189, 2509
BstYI RGATCY 3 cut(s) 1456, 2189, 2509
Bsu15I ATCGAT 1 cut(s) 410
BsuRI GGCC 8 cut(s) 229, 315, 370, 398, 776, 1591, 2228, 2417
BsuTUI ATCGAT 1 cut(s) 410
BtgZI GCGATG 1 cut(s) 1740
BtsIMutI CAGTG 3 cut(s) 1094, 2386, 2667
BtuMI TCGCGA 1 cut(s) 2217
Cac8I GCNNGC 5 cut(s) 160, 257, 723, 1607, 2562
CaiI CAGNNNCTG 2 cut(s) 45, 635
CciI TCATGA 2 cut(s) 1480, 2583
Cfr10I RCCGGY 2 cut(s) 158, 255
Cfr13I GGNCC 7 cut(s) 25, 228, 368, 369, 2341, 2349, 2416
ClaI ATCGAT 1 cut(s) 410
CseI GACGC 4 cut(s) 292, 327, 2084, 2222
Csp6I GTAC 7 cut(s) 49, 442, 652, 727, 877, 1733, 2552
CviAII CATG 9 cut(s) 221, 1481, 1606, 2252, 2283, 2368, 2413, 2522, 2584
CviQI GTAC 7 cut(s) 49, 442, 652, 727, 877, 1733, 2552
DrdI GACNNNNNNGTC 1 cut(s) 23
DseDI GACNNNNNNGTC 1 cut(s) 23
EaeI YGGCCR 1 cut(s) 1589
Eam1104I CTCTTC 2 cut(s) 696, 2457
EarI CTCTTC 2 cut(s) 696, 2457
Ecl136II GAGCTC 2 cut(s) 544, 1972
Eco105I TACGTA 1 cut(s) 880
Eco130I CCWWGG 3 cut(s) 1069, 1552, 2345
Eco24I GRGCYC 4 cut(s) 372, 546, 1247, 1974
Eco31I GGTCTC 1 cut(s) 616
Eco32I GATATC 1 cut(s) 1549
Eco47I GGWCC 3 cut(s) 25, 2341, 2349
Eco53kI GAGCTC 2 cut(s) 544, 1972
Eco57I CTGAAG 3 cut(s) 717, 1332, 1845
Eco72I CACGTG 1 cut(s) 1292
Eco88I CYCGRG 3 cut(s) 34, 667, 809
EcoICRI GAGCTC 2 cut(s) 544, 1972
EcoNI CCTNNNNNAGG 1 cut(s) 1268
EcoO109I RGGNCCY 1 cut(s) 228
EcoRII CCWGG 3 cut(s) 1522, 1960, 2337
EcoRV GATATC 1 cut(s) 1549
EcoT14I CCWWGG 3 cut(s) 1069, 1552, 2345
EcoT22I ATGCAT 1 cut(s) 1611
EcoT38I GRGCYC 4 cut(s) 372, 546, 1247, 1974
ErhI CCWWGG 3 cut(s) 1069, 1552, 2345
Esp3I CGTCTC 4 cut(s) 174, 670, 2240, 2641
FaeI CATG 9 cut(s) 224, 1484, 1609, 2255, 2286, 2371, 2416, 2525, 2587
FalI AAGNNNNNCTT 6 cut(s) 1536, 1568, 2098, 2130, 2496, 2528
FaqI GGGAC 4 cut(s) 11, 290, 617, 1931
FatI CATG 9 cut(s) 220, 1480, 1605, 2251, 2282, 2367, 2412, 2521, 2583
FauI CCCGC 2 cut(s) 239, 260
FbaI TGATCA 2 cut(s) 1327, 1626
Fnu4HI GCNGC 8 cut(s) 78, 146, 156, 269, 272, 479, 719, 1520
FriOI GRGCYC 4 cut(s) 372, 546, 1247, 1974
Fsp4HI GCNGC 8 cut(s) 78, 146, 156, 269, 272, 479, 719, 1520
FspBI CTAG 3 cut(s) 722, 1515, 1955
GluI GCNGC 8 cut(s) 78, 146, 156, 269, 272, 479, 719, 1520
GsaI CCCAGC 1 cut(s) 375
GsuI CTGGAG 1 cut(s) 1392
HaeIII GGCC 8 cut(s) 229, 315, 370, 398, 776, 1591, 2228, 2417
HapII CCGG 9 cut(s) 159, 179, 235, 256, 300, 342, 366, 563, 1697
HgaI GACGC 4 cut(s) 292, 327, 2084, 2222
Hin1I GRCGYC 3 cut(s) 303, 338, 2095
Hin1II CATG 9 cut(s) 224, 1484, 1609, 2255, 2286, 2371, 2416, 2525, 2587
HincII GTYRAC 3 cut(s) 244, 286, 2068
HindII GTYRAC 3 cut(s) 244, 286, 2068
HindIII AAGCTT 5 cut(s) 380, 497, 586, 2266, 2304
HpaII CCGG 9 cut(s) 159, 179, 235, 256, 300, 342, 366, 563, 1697
HphI GGTGA 4 cut(s) 51, 581, 672, 2692
Hpy166II GTNNAC 8 cut(s) 244, 286, 442, 458, 754, 873, 2068, 2564
Hpy8I GTNNAC 8 cut(s) 244, 286, 442, 458, 754, 873, 2068, 2564
Hpy99I CGWCG 2 cut(s) 314, 1757
HpyCH4III ACNGT 8 cut(s) 424, 731, 1098, 1357, 1471, 1596, 2072, 2556
HpyCH4IV ACGT 4 cut(s) 663, 875, 879, 1291
HpyF10VI GCNNNNNNNGC 6 cut(s) 265, 1955, 2177, 2429, 2500, 2615
HpySE526I ACGT 4 cut(s) 663, 875, 879, 1291
Hsp92I GRCGYC 3 cut(s) 303, 338, 2095
Hsp92II CATG 9 cut(s) 224, 1484, 1609, 2255, 2286, 2371, 2416, 2525, 2587
KroI GCCGGC 2 cut(s) 158, 255
KroNI GCCGGC 2 cut(s) 160, 257
Ksp22I TGATCA 2 cut(s) 1327, 1626
LmnI GCTCC 5 cut(s) 541, 1048, 1077, 1242, 1771
Lsp1109I GCAGC 4 cut(s) 132, 465, 730, 1531
LweI GCATC 9 cut(s) 948, 966, 985, 1327, 1630, 1676, 1859, 1936, 2596
MaeI CTAG 3 cut(s) 722, 1515, 1955
MaeII ACGT 4 cut(s) 663, 875, 879, 1291
MaeIII GTNAC 2 cut(s) 2245, 2381
MflI RGATCY 3 cut(s) 1456, 2189, 2509
MhlI GDGCHC 5 cut(s) 372, 546, 1247, 1974, 2566
MlsI TGGCCA 1 cut(s) 1591
MluNI TGGCCA 1 cut(s) 1591
MmeI TCCRAC 5 cut(s) 39, 1055, 1578, 1923, 2439
Mox20I TGGCCA 1 cut(s) 1591
Mph1103I ATGCAT 1 cut(s) 1611
MreI CGCCGGCG 2 cut(s) 158, 255
MroNI GCCGGC 2 cut(s) 158, 255
MroXI GAANNNNTTC 1 cut(s) 2109
MscI TGGCCA 1 cut(s) 1591
MseI TTAA 5 cut(s) 1064, 1361, 1440, 2052, 2138
MslI CAYNNNNRTG 1 cut(s) 2526
Msp20I TGGCCA 1 cut(s) 1591
MspA1I CMGCKG 2 cut(s) 23, 145
MspI CCGG 9 cut(s) 159, 179, 235, 256, 300, 342, 366, 563, 1697
MspR9I CCNGG 5 cut(s) 367, 1524, 1697, 1962, 2339
MvaI CCWGG 3 cut(s) 1524, 1962, 2339
MvnI CGCG 3 cut(s) 77, 274, 2217
MwoI GCNNNNNNNGC 6 cut(s) 265, 1955, 2177, 2429, 2500, 2615
NaeI GCCGGC 2 cut(s) 160, 257
NciI CCSGG 2 cut(s) 367, 1697
NgoMIV GCCGGC 2 cut(s) 158, 255
NheI GCTAGC 1 cut(s) 721
NlaIII CATG 9 cut(s) 224, 1484, 1609, 2255, 2286, 2371, 2416, 2525, 2587
NlaIV GGNNCC 6 cut(s) 27, 230, 370, 1244, 1966, 2343
NmuCI GTSAC 1 cut(s) 2381
NruI TCGCGA 1 cut(s) 2217
NsiI ATGCAT 1 cut(s) 1611
NspI RCATGY 2 cut(s) 1609, 2255
PaeI GCATGC 1 cut(s) 1609
PaeR7I CTCGAG 2 cut(s) 34, 667
PagI TCATGA 2 cut(s) 1480, 2583
PciI ACATGT 1 cut(s) 2251
PcsI WCGNNNNNNNCGW 1 cut(s) 2572
PdiI GCCGGC 2 cut(s) 160, 257
PdmI GAANNNNTTC 1 cut(s) 2109
PfeI GAWTC 8 cut(s) 105, 137, 887, 1504, 1894, 2129, 2183, 2212
Pfl23II CGTACG 1 cut(s) 876
PflFI GACNNNGTC 1 cut(s) 662
PfoI TCCNGGA 1 cut(s) 2337
PkrI GCNGC 8 cut(s) 79, 147, 157, 270, 273, 480, 720, 1521
Ple19I CGATCG 1 cut(s) 332
PmaCI CACGTG 1 cut(s) 1292
PmlI CACGTG 1 cut(s) 1292
Ppu21I YACGTR 2 cut(s) 880, 1292
PscI ACATGT 1 cut(s) 2251
PsiI TTATAA 1 cut(s) 2324
Psp124BI GAGCTC 2 cut(s) 546, 1974
Psp6I CCWGG 3 cut(s) 1522, 1960, 2337
PspCI CACGTG 1 cut(s) 1292
PspFI CCCAGC 1 cut(s) 371
PspGI CCWGG 3 cut(s) 1522, 1960, 2337
PspLI CGTACG 1 cut(s) 876
PspN4I GGNNCC 6 cut(s) 27, 230, 370, 1244, 1966, 2343
PspOMI GGGCCC 1 cut(s) 368
PspPI GGNCC 7 cut(s) 25, 228, 368, 369, 2341, 2349, 2416
PspXI VCTCGAGB 1 cut(s) 34
PstI CTGCAG 2 cut(s) 483, 1774
PstNI CAGNNNCTG 2 cut(s) 45, 635
PsuI RGATCY 3 cut(s) 1456, 2189, 2509
PsyI GACNNNGTC 1 cut(s) 662
PvuI CGATCG 1 cut(s) 332
PvuII CAGCTG 1 cut(s) 145
RruI TCGCGA 1 cut(s) 2217
RsaI GTAC 7 cut(s) 50, 443, 653, 728, 878, 1734, 2553
RsaNI GTAC 7 cut(s) 49, 442, 652, 727, 877, 1733, 2552
RseI CAYNNNNRTG 1 cut(s) 2526
SacI GAGCTC 2 cut(s) 546, 1974
SaqAI TTAA 5 cut(s) 1064, 1361, 1440, 2052, 2138
SatI GCNGC 8 cut(s) 78, 146, 156, 269, 272, 479, 719, 1520
Sau96I GGNCC 7 cut(s) 25, 228, 368, 369, 2341, 2349, 2416
ScaI AGTACT 2 cut(s) 1734, 2553
ScrFI CCNGG 5 cut(s) 367, 1524, 1697, 1962, 2339
SduI GDGCHC 5 cut(s) 372, 546, 1247, 1974, 2566
SfaNI GCATC 9 cut(s) 948, 966, 985, 1327, 1630, 1676, 1859, 1936, 2596
SfcI CTRYAG 2 cut(s) 479, 1770
Sfr274I CTCGAG 2 cut(s) 34, 667
SgrAI CRCCGGYG 2 cut(s) 158, 255
SinI GGWCC 3 cut(s) 25, 2341, 2349
SlaI CTCGAG 2 cut(s) 34, 667
SmiMI CAYNNNNRTG 1 cut(s) 2526
SmlI CTYRAG 3 cut(s) 8, 34, 667
SmoI CTYRAG 3 cut(s) 8, 34, 667
SnaBI TACGTA 1 cut(s) 880
SphI GCATGC 1 cut(s) 1609
SspMI CTAG 3 cut(s) 722, 1515, 1955
SstI GAGCTC 2 cut(s) 546, 1974
StyD4I CCNGG 5 cut(s) 365, 1522, 1695, 1960, 2337
StyI CCWWGG 3 cut(s) 1069, 1552, 2345
TaaI ACNGT 8 cut(s) 424, 731, 1098, 1357, 1471, 1596, 2072, 2556
TaiI ACGT 4 cut(s) 666, 878, 882, 1294
TaqI TCGA 6 cut(s) 35, 410, 668, 1283, 2132, 2396
TatI WGTACW 3 cut(s) 651, 1732, 2551
TauI GCSGC 4 cut(s) 80, 158, 271, 274
TfiI GAWTC 8 cut(s) 105, 137, 887, 1504, 1894, 2129, 2183, 2212
Tru1I TTAA 5 cut(s) 1064, 1361, 1440, 2052, 2138
Tru9I TTAA 5 cut(s) 1064, 1361, 1440, 2052, 2138
TscAI CASTG 3 cut(s) 1101, 2386, 2667
TseFI GTSAC 1 cut(s) 2381
TseI GCWGC 4 cut(s) 145, 478, 718, 1519
Tsp45I GTSAC 1 cut(s) 2381
TspDTI ATGAA 8 cut(s) 58, 237, 343, 1743, 1846, 2271, 2538, 2562
TspRI CASTG 3 cut(s) 1101, 2386, 2667
Tth111I GACNNNGTC 1 cut(s) 662
VneI GTGCAC 1 cut(s) 2562
VpaK11BI GGWCC 3 cut(s) 25, 2341, 2349
XagI CCTNNNNNAGG 1 cut(s) 1268
XapI RAATTY 3 cut(s) 448, 503, 1863
XceI RCATGY 2 cut(s) 1609, 2255
XcmI CCANNNNNNNNNTGG 1 cut(s) 1126
XhoI CTCGAG 2 cut(s) 34, 667
XmnI GAANNNNTTC 1 cut(s) 2109
XspI CTAG 3 cut(s) 722, 1515, 1955
ZrmI AGTACT 2 cut(s) 1734, 2553
Zsp2I ATGCAT 1 cut(s) 1611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.