Ion channel CASTOR-like
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2264 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 23 |
| AccB1I | GGYRCC | 1 cut(s) | 213 |
| AccI | GTMKAC | 1 cut(s) | 1795 |
| AccII | CGCG | 3 cut(s) | 162, 259, 2502 |
| AccIII | TCCGGA | 2 cut(s) | 350, 1690 |
| AciI | CCGC | 8 cut(s) | 62, 183, 217, 244, 254, 257, 845, 1852 |
| AclWI | GGATC | 2 cut(s) | 1093, 2385 |
| AcoI | YGGCCR | 2 cut(s) | 1583, 2085 |
| AcsI | RAATTY | 2 cut(s) | 2274, 2296 |
| AcuI | CTGAAG | 3 cut(s) | 711, 1326, 2232 |
| AcvI | CACGTG | 1 cut(s) | 1286 |
| AcyI | GRCGYC | 1 cut(s) | 214 |
| AfaI | GTAC | 7 cut(s) | 50, 428, 647, 722, 1617, 1728, 2493 |
| AfeI | AGCGCT | 1 cut(s) | 597 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 243, 244, 312, 634, 1264, 1914, 2511 |
| AflIII | ACRYGT | 1 cut(s) | 2191 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 1167, 1199, 1530, 1562 |
| Alw21I | GWGCWC | 2 cut(s) | 531, 1914 |
| Alw26I | GTCTC | 7 cut(s) | 36, 610, 664, 1152, 1279, 2180, 2581 |
| AlwI | GGATC | 2 cut(s) | 1093, 2385 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 45 |
| Ama87I | CYCGRG | 2 cut(s) | 661, 1900 |
| Aor13HI | TCCGGA | 2 cut(s) | 350, 1690 |
| Aor51HI | AGCGCT | 1 cut(s) | 597 |
| AoxI | GGCC | 4 cut(s) | 298, 1583, 1896, 2085 |
| ApeKI | GCWGC | 4 cut(s) | 77, 1513, 1766, 2239 |
| ApoI | RAATTY | 2 cut(s) | 2274, 2296 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 2049, 2209 |
| AspLEI | GCGC | 5 cut(s) | 164, 216, 598, 2502, 2504 |
| AspS9I | GGNCC | 2 cut(s) | 1897, 2281 |
| AsuC2I | CCSGG | 3 cut(s) | 1518, 1901, 1902 |
| AsuHPI | GGTGA | 4 cut(s) | 51, 666, 2480, 2632 |
| AsuNHI | GCTAGC | 1 cut(s) | 715 |
| AvaI | CYCGRG | 2 cut(s) | 661, 1900 |
| AvaII | GGWCC | 1 cut(s) | 2281 |
| BalI | TGGCCA | 1 cut(s) | 1585 |
| BanI | GGYRCC | 1 cut(s) | 213 |
| BanII | GRGCYC | 3 cut(s) | 531, 1241, 1914 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 419, 451 |
| BbrPI | CACGTG | 1 cut(s) | 1286 |
| BbsI | GAAGAC | 3 cut(s) | 1314, 2042, 2109 |
| Bbv12I | GWGCWC | 2 cut(s) | 531, 1914 |
| BbvCI | CCTCAGC | 1 cut(s) | 1382 |
| BbvI | GCAGC | 4 cut(s) | 89, 1525, 1778, 2251 |
| BccI | CCATC | 5 cut(s) | 133, 143, 1575, 1589, 1958 |
| BceAI | ACGGC | 4 cut(s) | 139, 149, 313, 2019 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1739, 1773 |
| BciVI | GTATCC | 1 cut(s) | 1827 |
| BclI | TGATCA | 2 cut(s) | 1321, 1620 |
| BcnI | CCSGG | 3 cut(s) | 1518, 1901, 1902 |
| BcoDI | GTCTC | 7 cut(s) | 36, 610, 664, 1152, 1279, 2180, 2581 |
| BfaI | CTAG | 2 cut(s) | 716, 1509 |
| BfmI | CTRYAG | 2 cut(s) | 1764, 1824 |
| BfoI | RGCGCY | 2 cut(s) | 217, 599 |
| BfuI | GTATCC | 1 cut(s) | 1827 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1895 |
| BglII | AGATCT | 4 cut(s) | 1450, 1530, 2129, 2449 |
| BisI | GCNGC | 8 cut(s) | 78, 217, 254, 257, 1514, 1767, 1853, 2240 |
| BlpI | GCTNAGC | 4 cut(s) | 841, 1152, 2169, 2539 |
| BlsI | GCNGC | 8 cut(s) | 79, 218, 255, 258, 1515, 1768, 1854, 2241 |
| BmcAI | AGTACT | 2 cut(s) | 1728, 2493 |
| Bme1390I | CCNGG | 3 cut(s) | 1518, 1901, 1902 |
| Bme18I | GGWCC | 1 cut(s) | 2281 |
| BmeT110I | CYCGRG | 2 cut(s) | 661, 1900 |
| BmgT120I | GGNCC | 2 cut(s) | 1897, 2281 |
| BmiI | GGNNCC | 5 cut(s) | 215, 1238, 1899, 1906, 2283 |
| BmrFI | CCNGG | 3 cut(s) | 1518, 1901, 1902 |
| BmsI | GCATC | 8 cut(s) | 942, 960, 979, 1321, 1624, 1670, 1876, 2536 |
| BmtI | GCTAGC | 1 cut(s) | 719 |
| BpiI | GAAGAC | 3 cut(s) | 1314, 2042, 2109 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 671, 703 |
| BpmI | CTGGAG | 2 cut(s) | 19, 575 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1382 |
| Bpu1102I | GCTNAGC | 4 cut(s) | 841, 1152, 2169, 2539 |
| BpuMI | CCSGG | 3 cut(s) | 1518, 1901, 1902 |
| BsaAI | YACGTR | 1 cut(s) | 1286 |
| BsaBI | GATNNNNATC | 2 cut(s) | 564, 2454 |
| BsaHI | GRCGYC | 1 cut(s) | 214 |
| BsaI | GGTCTC | 1 cut(s) | 610 |
| BsaJI | CCNNGG | 5 cut(s) | 1278, 1546, 1899, 1900, 2285 |
| BsaWI | WCCGGW | 5 cut(s) | 350, 369, 676, 1364, 1690 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 243, 244, 312, 634, 1264, 1914, 2511 |
| Bse1I | ACTGG | 3 cut(s) | 50, 984, 2364 |
| Bse3DI | GCAATG | 3 cut(s) | 972, 1769, 2141 |
| Bse8I | GATNNNNATC | 2 cut(s) | 564, 2454 |
| BseAI | TCCGGA | 2 cut(s) | 350, 1690 |
| BseDI | CCNNGG | 5 cut(s) | 1278, 1546, 1899, 1900, 2285 |
| BseJI | GATNNNNATC | 2 cut(s) | 564, 2454 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 243, 244, 312, 634, 1264, 1914, 2511 |
| BseMI | GCAATG | 3 cut(s) | 972, 1769, 2141 |
| BseMII | CTCAG | 7 cut(s) | 33, 164, 1143, 1373, 1926, 2121, 2541 |
| BseNI | ACTGG | 3 cut(s) | 50, 984, 2364 |
| BsePI | GCGCGC | 1 cut(s) | 2500 |
| BseRI | GAGGAG | 2 cut(s) | 2432, 2486 |
| BseXI | GCAGC | 4 cut(s) | 89, 1525, 1778, 2251 |
| BseYI | CCCAGC | 1 cut(s) | 547 |
| Bsh1236I | CGCG | 3 cut(s) | 162, 259, 2502 |
| Bsh1285I | CGRYCG | 1 cut(s) | 317 |
| BshFI | GGCC | 4 cut(s) | 300, 1585, 1898, 2087 |
| BshNI | GGYRCC | 1 cut(s) | 213 |
| BsiEI | CGRYCG | 1 cut(s) | 317 |
| BsiHKAI | GWGCWC | 2 cut(s) | 531, 1914 |
| BsiHKCI | CYCGRG | 2 cut(s) | 661, 1900 |
| BslFI | GGGAC | 3 cut(s) | 11, 275, 1401 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 243, 244, 312, 634, 1264, 1914, 2511 |
| BsmAI | GTCTC | 7 cut(s) | 36, 610, 664, 1152, 1279, 2180, 2581 |
| BsmBI | CGTCTC | 2 cut(s) | 2180, 2581 |
| BsmFI | GGGAC | 3 cut(s) | 11, 275, 1401 |
| BsnI | GGCC | 4 cut(s) | 300, 1585, 1898, 2087 |
| Bso31I | GGTCTC | 1 cut(s) | 610 |
| BsoBI | CYCGRG | 2 cut(s) | 661, 1900 |
| Bsp1286I | GDGCHC | 3 cut(s) | 531, 1241, 1914 |
| Bsp13I | TCCGGA | 2 cut(s) | 350, 1690 |
| Bsp1720I | GCTNAGC | 4 cut(s) | 841, 1152, 2169, 2539 |
| BspACI | CCGC | 8 cut(s) | 62, 183, 217, 244, 254, 257, 845, 1852 |
| BspANI | GGCC | 4 cut(s) | 300, 1585, 1898, 2087 |
| BspCNI | CTCAG | 7 cut(s) | 32, 163, 1144, 1374, 1927, 2120, 2542 |
| BspEI | TCCGGA | 2 cut(s) | 350, 1690 |
| BspFNI | CGCG | 3 cut(s) | 162, 259, 2502 |
| BspHI | TCATGA | 2 cut(s) | 1474, 2523 |
| BspLI | GGNNCC | 5 cut(s) | 215, 1238, 1899, 1906, 2283 |
| BspMAI | CTGCAG | 2 cut(s) | 1768, 1828 |
| BspOI | GCTAGC | 1 cut(s) | 719 |
| BspPI | GGATC | 2 cut(s) | 1093, 2385 |
| BspQI | GCTCTTC | 1 cut(s) | 2397 |
| BspT107I | GGYRCC | 1 cut(s) | 213 |
| BspTNI | GGTCTC | 1 cut(s) | 610 |
| BsrDI | GCAATG | 3 cut(s) | 972, 1769, 2141 |
| BsrI | ACTGG | 3 cut(s) | 50, 984, 2364 |
| BssECI | CCNNGG | 5 cut(s) | 1278, 1546, 1899, 1900, 2285 |
| BssHII | GCGCGC | 1 cut(s) | 2500 |
| BssNI | GRCGYC | 1 cut(s) | 214 |
| BssT1I | CCWWGG | 3 cut(s) | 1278, 1546, 2285 |
| Bst6I | CTCTTC | 1 cut(s) | 2397 |
| BstACI | GRCGYC | 1 cut(s) | 214 |
| BstBAI | YACGTR | 1 cut(s) | 1286 |
| BstC8I | GCNNGC | 4 cut(s) | 717, 1601, 2244, 2502 |
| BstEII | GGTNACC | 1 cut(s) | 672 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1262 |
| BstFNI | CGCG | 3 cut(s) | 162, 259, 2502 |
| BstH2I | RGCGCY | 2 cut(s) | 217, 599 |
| BstHHI | GCGC | 5 cut(s) | 164, 216, 598, 2502, 2504 |
| BstMAI | GTCTC | 7 cut(s) | 36, 610, 664, 1152, 1279, 2180, 2581 |
| BstMCI | CGRYCG | 1 cut(s) | 317 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 250, 1766, 1895, 2117, 2369, 2440, 2555 |
| BstNSI | RCATGY | 2 cut(s) | 1603, 2195 |
| BstPI | GGTNACC | 1 cut(s) | 672 |
| BstSCI | CCNGG | 3 cut(s) | 1516, 1899, 1900 |
| BstSFI | CTRYAG | 2 cut(s) | 1764, 1824 |
| BstUI | CGCG | 3 cut(s) | 162, 259, 2502 |
| BstV1I | GCAGC | 4 cut(s) | 89, 1525, 1778, 2251 |
| BstV2I | GAAGAC | 3 cut(s) | 1314, 2042, 2109 |
| BstX2I | RGATCY | 4 cut(s) | 1450, 1530, 2129, 2449 |
| BstYI | RGATCY | 4 cut(s) | 1450, 1530, 2129, 2449 |
| BsuI | GTATCC | 1 cut(s) | 1827 |
| BsuRI | GGCC | 4 cut(s) | 300, 1585, 1898, 2087 |
| BtgZI | GCGATG | 1 cut(s) | 1734 |
| BtsIMutI | CAGTG | 5 cut(s) | 426, 764, 785, 2357, 2607 |
| Cac8I | GCNNGC | 4 cut(s) | 717, 1601, 2244, 2502 |
| CaiI | CAGNNNCTG | 1 cut(s) | 45 |
| CciI | TCATGA | 2 cut(s) | 1474, 2523 |
| CfoI | GCGC | 5 cut(s) | 164, 216, 598, 2502, 2504 |
| Cfr13I | GGNCC | 2 cut(s) | 1897, 2281 |
| Cfr9I | CCCGGG | 1 cut(s) | 1900 |
| CseI | GACGC | 2 cut(s) | 277, 2162 |
| Csp6I | GTAC | 7 cut(s) | 49, 427, 646, 721, 1616, 1727, 2492 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 753, 788 |
| CviQI | GTAC | 7 cut(s) | 49, 427, 646, 721, 1616, 1727, 2492 |
| DinI | GGCGCC | 1 cut(s) | 215 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 23 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 23 |
| EaeI | YGGCCR | 2 cut(s) | 1583, 2085 |
| Eam1104I | CTCTTC | 1 cut(s) | 2397 |
| EarI | CTCTTC | 1 cut(s) | 2397 |
| EciI | GGCGGA | 1 cut(s) | 172 |
| Ecl136II | GAGCTC | 2 cut(s) | 529, 1912 |
| Eco130I | CCWWGG | 3 cut(s) | 1278, 1546, 2285 |
| Eco24I | GRGCYC | 3 cut(s) | 531, 1241, 1914 |
| Eco31I | GGTCTC | 1 cut(s) | 610 |
| Eco32I | GATATC | 1 cut(s) | 1543 |
| Eco47I | GGWCC | 1 cut(s) | 2281 |
| Eco47III | AGCGCT | 1 cut(s) | 597 |
| Eco53kI | GAGCTC | 2 cut(s) | 529, 1912 |
| Eco57I | CTGAAG | 3 cut(s) | 711, 1326, 2232 |
| Eco72I | CACGTG | 1 cut(s) | 1286 |
| Eco88I | CYCGRG | 2 cut(s) | 661, 1900 |
| Eco91I | GGTNACC | 1 cut(s) | 672 |
| EcoICRI | GAGCTC | 2 cut(s) | 529, 1912 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1262 |
| EcoO65I | GGTNACC | 1 cut(s) | 672 |
| EcoRV | GATATC | 1 cut(s) | 1543 |
| EcoT14I | CCWWGG | 3 cut(s) | 1278, 1546, 2285 |
| EcoT22I | ATGCAT | 1 cut(s) | 1605 |
| EcoT38I | GRGCYC | 3 cut(s) | 531, 1241, 1914 |
| EgeI | GGCGCC | 1 cut(s) | 215 |
| EheI | GGCGCC | 1 cut(s) | 215 |
| ErhI | CCWWGG | 3 cut(s) | 1278, 1546, 2285 |
| Esp3I | CGTCTC | 2 cut(s) | 2180, 2581 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1530, 1562, 2038, 2070 |
| FaqI | GGGAC | 3 cut(s) | 11, 275, 1401 |
| FauI | CCCGC | 1 cut(s) | 237 |
| FbaI | TGATCA | 2 cut(s) | 1321, 1620 |
| FblI | GTMKAC | 1 cut(s) | 1795 |
| Fnu4HI | GCNGC | 8 cut(s) | 78, 217, 254, 257, 1514, 1767, 1853, 2240 |
| FriOI | GRGCYC | 3 cut(s) | 531, 1241, 1914 |
| Fsp4HI | GCNGC | 8 cut(s) | 78, 217, 254, 257, 1514, 1767, 1853, 2240 |
| FspBI | CTAG | 2 cut(s) | 716, 1509 |
| GlaI | GCGC | 5 cut(s) | 163, 215, 597, 2501, 2503 |
| GluI | GCNGC | 8 cut(s) | 78, 217, 254, 257, 1514, 1767, 1853, 2240 |
| GsaI | CCCAGC | 1 cut(s) | 551 |
| GsuI | CTGGAG | 2 cut(s) | 19, 575 |
| HaeII | RGCGCY | 2 cut(s) | 217, 599 |
| HaeIII | GGCC | 4 cut(s) | 300, 1585, 1898, 2087 |
| HgaI | GACGC | 2 cut(s) | 277, 2162 |
| HhaI | GCGC | 5 cut(s) | 164, 216, 598, 2502, 2504 |
| Hin1I | GRCGYC | 1 cut(s) | 214 |
| Hin6I | GCGC | 5 cut(s) | 162, 214, 596, 2500, 2502 |
| HinP1I | GCGC | 5 cut(s) | 162, 214, 596, 2500, 2502 |
| HincII | GTYRAC | 3 cut(s) | 229, 271, 2008 |
| HindII | GTYRAC | 3 cut(s) | 229, 271, 2008 |
| HindIII | AAGCTT | 4 cut(s) | 482, 2206, 2244, 2479 |
| HphI | GGTGA | 4 cut(s) | 51, 666, 2480, 2632 |
| Hpy166II | GTNNAC | 8 cut(s) | 229, 271, 427, 443, 748, 867, 1796, 2008 |
| Hpy8I | GTNNAC | 8 cut(s) | 229, 271, 427, 443, 748, 867, 1796, 2008 |
| Hpy99I | CGWCG | 2 cut(s) | 243, 299 |
| HpyAV | CCTTC | 8 cut(s) | 39, 76, 460, 1709, 1933, 2459, 2599, 2626 |
| HpyCH4IV | ACGT | 2 cut(s) | 869, 1285 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 250, 1766, 1895, 2117, 2369, 2440, 2555 |
| HpySE526I | ACGT | 2 cut(s) | 869, 1285 |
| Hsp92I | GRCGYC | 1 cut(s) | 214 |
| HspAI | GCGC | 5 cut(s) | 162, 214, 596, 2500, 2502 |
| KasI | GGCGCC | 1 cut(s) | 213 |
| Kpn2I | TCCGGA | 2 cut(s) | 350, 1690 |
| Ksp22I | TGATCA | 2 cut(s) | 1321, 1620 |
| LguI | GCTCTTC | 1 cut(s) | 2397 |
| LmnI | GCTCC | 6 cut(s) | 185, 526, 1042, 1071, 1236, 1765 |
| Lsp1109I | GCAGC | 4 cut(s) | 89, 1525, 1778, 2251 |
| LweI | GCATC | 8 cut(s) | 942, 960, 979, 1321, 1624, 1670, 1876, 2536 |
| MaeI | CTAG | 2 cut(s) | 716, 1509 |
| MaeII | ACGT | 2 cut(s) | 869, 1285 |
| MaeIII | GTNAC | 4 cut(s) | 672, 789, 2185, 2423 |
| MflI | RGATCY | 4 cut(s) | 1450, 1530, 2129, 2449 |
| MhlI | GDGCHC | 3 cut(s) | 531, 1241, 1914 |
| MlsI | TGGCCA | 1 cut(s) | 1585 |
| MluNI | TGGCCA | 1 cut(s) | 1585 |
| Mly113I | GGCGCC | 1 cut(s) | 214 |
| MlyI | GAGTC | 9 cut(s) | 5, 11, 348, 893, 1004, 1659, 1691, 2099, 2102 |
| MmeI | TCCRAC | 5 cut(s) | 39, 297, 1049, 1572, 1863 |
| Mox20I | TGGCCA | 1 cut(s) | 1585 |
| Mph1103I | ATGCAT | 1 cut(s) | 1605 |
| MroI | TCCGGA | 2 cut(s) | 350, 1690 |
| MroXI | GAANNNNTTC | 2 cut(s) | 2049, 2209 |
| MscI | TGGCCA | 1 cut(s) | 1585 |
| MseI | TTAA | 6 cut(s) | 584, 1058, 1355, 1434, 2078, 2477 |
| MslI | CAYNNNNRTG | 1 cut(s) | 2466 |
| Msp20I | TGGCCA | 1 cut(s) | 1585 |
| MspR9I | CCNGG | 3 cut(s) | 1518, 1901, 1902 |
| MvnI | CGCG | 3 cut(s) | 162, 259, 2502 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 250, 1766, 1895, 2117, 2369, 2440, 2555 |
| NarI | GGCGCC | 1 cut(s) | 214 |
| NciI | CCSGG | 3 cut(s) | 1518, 1901, 1902 |
| NheI | GCTAGC | 1 cut(s) | 715 |
| NlaIV | GGNNCC | 5 cut(s) | 215, 1238, 1899, 1906, 2283 |
| NmeAIII | GCCGAG | 1 cut(s) | 336 |
| NmuCI | GTSAC | 1 cut(s) | 672 |
| NsiI | ATGCAT | 1 cut(s) | 1605 |
| NspI | RCATGY | 2 cut(s) | 1603, 2195 |
| PaeI | GCATGC | 1 cut(s) | 1603 |
| PaeR7I | CTCGAG | 1 cut(s) | 661 |
| PagI | TCATGA | 2 cut(s) | 1474, 2523 |
| PauI | GCGCGC | 1 cut(s) | 2500 |
| PciI | ACATGT | 1 cut(s) | 2191 |
| PciSI | GCTCTTC | 1 cut(s) | 2397 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1071 |
| PdmI | GAANNNNTTC | 2 cut(s) | 2049, 2209 |
| PflFI | GACNNNGTC | 2 cut(s) | 335, 656 |
| PkrI | GCNGC | 8 cut(s) | 79, 218, 255, 258, 1515, 1768, 1854, 2241 |
| PleI | GAGTC | 9 cut(s) | 5, 11, 348, 893, 1004, 1658, 1691, 2099, 2101 |
| PluTI | GGCGCC | 1 cut(s) | 217 |
| PmaCI | CACGTG | 1 cut(s) | 1286 |
| PmlI | CACGTG | 1 cut(s) | 1286 |
| PpsI | GAGTC | 9 cut(s) | 5, 11, 348, 893, 1004, 1658, 1691, 2099, 2101 |
| Ppu21I | YACGTR | 1 cut(s) | 1286 |
| PscI | ACATGT | 1 cut(s) | 2191 |
| PsiI | TTATAA | 1 cut(s) | 2264 |
| Psp124BI | GAGCTC | 2 cut(s) | 531, 1914 |
| PspCI | CACGTG | 1 cut(s) | 1286 |
| PspEI | GGTNACC | 1 cut(s) | 672 |
| PspFI | CCCAGC | 1 cut(s) | 547 |
| PspN4I | GGNNCC | 5 cut(s) | 215, 1238, 1899, 1906, 2283 |
| PspPI | GGNCC | 2 cut(s) | 1897, 2281 |
| PstI | CTGCAG | 2 cut(s) | 1768, 1828 |
| PstNI | CAGNNNCTG | 1 cut(s) | 45 |
| PsuI | RGATCY | 4 cut(s) | 1450, 1530, 2129, 2449 |
| PsyI | GACNNNGTC | 2 cut(s) | 335, 656 |
| PteI | GCGCGC | 1 cut(s) | 2500 |
| RsaI | GTAC | 7 cut(s) | 50, 428, 647, 722, 1617, 1728, 2493 |
| RsaNI | GTAC | 7 cut(s) | 49, 427, 646, 721, 1616, 1727, 2492 |
| RseI | CAYNNNNRTG | 1 cut(s) | 2466 |
| SacI | GAGCTC | 2 cut(s) | 531, 1914 |
| SapI | GCTCTTC | 1 cut(s) | 2397 |
| SaqAI | TTAA | 6 cut(s) | 584, 1058, 1355, 1434, 2078, 2477 |
| SatI | GCNGC | 8 cut(s) | 78, 217, 254, 257, 1514, 1767, 1853, 2240 |
| Sau96I | GGNCC | 2 cut(s) | 1897, 2281 |
| ScaI | AGTACT | 2 cut(s) | 1728, 2493 |
| SchI | GAGTC | 9 cut(s) | 5, 11, 348, 893, 1004, 1659, 1691, 2099, 2102 |
| ScrFI | CCNGG | 3 cut(s) | 1518, 1901, 1902 |
| SduI | GDGCHC | 3 cut(s) | 531, 1241, 1914 |
| SfaNI | GCATC | 8 cut(s) | 942, 960, 979, 1321, 1624, 1670, 1876, 2536 |
| SfcI | CTRYAG | 2 cut(s) | 1764, 1824 |
| SfoI | GGCGCC | 1 cut(s) | 215 |
| Sfr274I | CTCGAG | 1 cut(s) | 661 |
| SinI | GGWCC | 1 cut(s) | 2281 |
| SlaI | CTCGAG | 1 cut(s) | 661 |
| SmaI | CCCGGG | 1 cut(s) | 1902 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 2466 |
| SmlI | CTYRAG | 1 cut(s) | 661 |
| SmoI | CTYRAG | 1 cut(s) | 661 |
| SphI | GCATGC | 1 cut(s) | 1603 |
| SsiI | CCGC | 8 cut(s) | 62, 183, 217, 244, 254, 257, 845, 1852 |
| SspDI | GGCGCC | 1 cut(s) | 213 |
| SspMI | CTAG | 2 cut(s) | 716, 1509 |
| SstI | GAGCTC | 2 cut(s) | 531, 1914 |
| StyD4I | CCNGG | 3 cut(s) | 1516, 1899, 1900 |
| StyI | CCWWGG | 3 cut(s) | 1278, 1546, 2285 |
| TaiI | ACGT | 2 cut(s) | 872, 1288 |
| TaqI | TCGA | 5 cut(s) | 9, 662, 729, 2072, 2336 |
| TatI | WGTACW | 4 cut(s) | 645, 1615, 1726, 2491 |
| TauI | GCSGC | 4 cut(s) | 219, 256, 259, 1855 |
| Tru1I | TTAA | 6 cut(s) | 584, 1058, 1355, 1434, 2078, 2477 |
| Tru9I | TTAA | 6 cut(s) | 584, 1058, 1355, 1434, 2078, 2477 |
| TscAI | CASTG | 5 cut(s) | 426, 771, 792, 2364, 2607 |
| TseFI | GTSAC | 1 cut(s) | 672 |
| TseI | GCWGC | 4 cut(s) | 77, 1513, 1766, 2239 |
| Tsp45I | GTSAC | 1 cut(s) | 672 |
| TspDTI | ATGAA | 6 cut(s) | 58, 1737, 1941, 2211, 2478, 2502 |
| TspGWI | ACGGA | 2 cut(s) | 1326, 2605 |
| TspMI | CCCGGG | 1 cut(s) | 1900 |
| TspRI | CASTG | 5 cut(s) | 426, 771, 792, 2364, 2607 |
| Tth111I | GACNNNGTC | 2 cut(s) | 335, 656 |
| VpaK11BI | GGWCC | 1 cut(s) | 2281 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1262 |
| XapI | RAATTY | 2 cut(s) | 2274, 2296 |
| XceI | RCATGY | 2 cut(s) | 1603, 2195 |
| XhoI | CTCGAG | 1 cut(s) | 661 |
| XmaI | CCCGGG | 1 cut(s) | 1900 |
| XmiI | GTMKAC | 1 cut(s) | 1795 |
| XmnI | GAANNNNTTC | 2 cut(s) | 2049, 2209 |
| XspI | CTAG | 2 cut(s) | 716, 1509 |
| ZrmI | AGTACT | 2 cut(s) | 1728, 2493 |
| Zsp2I | ATGCAT | 1 cut(s) | 1605 |