Rh4CG140500

Ion channel CASTOR-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001000
Physical Location & Seq
Reverse (-)
27881859 .. 27890685
8827 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG140500.1

Sequence Viewer

Length: 2655 bp
ATGTCCCTCGACTCCGACTCAGCAGTCCCTTCCTCCAGCAGAGACTGGTACTTTCCCTCACCGCCCTTCATCCACGCAGCGAAATCCCCAAAATACCACCGAAGATTCCCCACAAACCCCAGACCATCCCCACCCCTCCCCACCGATTCTCAGCCGCCATTTGGTCCGGCGACGCCGTTTCGCGCCGTTTCGTCCTCCAGCTCCGCCCCTCCGAGAACTTTCAGCCATGACAGGCCCCGCCGGAGAGTTGACTTCTCCCGTCGGCGGGAGAAGCCGCCGCGAAACGATGTCAACCAGCCCGTTTCCGGCGTCCCCGACGGCCTCTCCAAAAAACGATCGGAGGCTTCCGGCGACAAGGTCATTAGCTTTTCCGGACTCGGCTTCAAACTCCGGTGGAAAATGGCTTGTTCAGTAGCGATTTTGATGACGGTTTTGTCATCAGTGGTGTACCAAAACTTCTGTTTACATAGCCAAGTCAATGAGTTGCAGGATTATATTTCCAAGCTTGAATTGAGATTACAAGCCTACAATTCGTTAGAATCTATGGAGCTCATCACTTCTTCTGGGCTGGAGGATGATGATCTTCCTAACAGAAGATTAAAACGATTAGCGCTTATGGTCTCCCTCATGTTTTTATCAATCCCTCTTGTGGTTTTCAAGTACATTGACTATGTCTCGAGATCACGGTCACCGGAGAACATCTCTGAAGAAGAATCTCTAAACAAACAGCTAGCGTACCGTGTCGATTTGTTTTTATCAGTCCACCCGTATTCTAAGCCACTGACATTGTTGGTTGCAACACTGTTACTTATTTGTCTTGGGGGGTTAGCATTGTTTGGAGTGAAAGATGATAGCTTAGCGGAATGTCTTTGGTTATCGTGGACGTATGTAGCTGATTCTGGCAACCATACTGACTCTGAAAAAATTGGCGAAAGGCTTGTTTCAGTTTCCATTAGCTTTGGTGGGATGCTCATATTTGCAATGATGCTTGGACTTGTTTCTGATGCAATTTCGGAGAAGTTTGACTCATTGAGGAAAGGAAGAAGTGAGGTTGTGGAGCAAAACCATACTTTAATTCTCGGTTGGAGCGATAAATTGGGATCATTATTGAACCAGATTGCAATAGCTAATGAGAGTTTGGGTGGAGGGATTGTTGTAGTTATGGCTGAGCGAGACAAAGAGGAAATGGAACTTGACATTGGTAAAATGGAGTTTGATTTCAAAGGAACATCTGTAATATGCAGAAGTGGGAGCCCTCTTATTCTTGCTGACCTGAAAAAGGTATCTGTCTCCAAGGCACGTGCAATAATTGTCCTTGCTGAAGACGGAAATGCTGATCAGAGTGATGCCCGTGCATTGAGAACTGTTTTAAGTCTAACCGGAGTGAAAGAGGGGCTGAGGGGACATATTGTAGTTGAACTAAGTGATCTTGACAATGAGGTTCTTGTTAAACTTGTTGGTGGAGATCTTGTTGAAACTGTTGTGGCTCATGATGTAATTGGTCGTCTGATGATTCAATGTGCTAGGCAGCCCGGACTAGCACAGATCTGGGAAGATATCCTTGGATTTGAAAATTGCGAGTTCTATATCAAAAGATGGCCACAGTTGGATGGCATGCATTTTGAGGATGTACTGATCAGTTTTCCTGATGCCATTCCTTGTGGAGTCAAGGTTGCATCATTGGGTGGCAGAATTATTTTGAACCCGGATGACTCTTATGTTCTACAAGAAGGCGATGAAGTACTTGTCATAGCAGAGGATGACGATACCTATGCTCCTGCAGCATTGCCTATGGTATGGAGAGGAAGTCTACCCAAAGACTTTGTTGTTCCAAAGTCTGCAGAAAGGATACTTTTATGTGGTTGGCGGCGAGATATGGAGGATATGATTATGGTGTTGGATGCCTTTCTGGCCCCGGGTTCCGAGCTCTGGATGTTCAATGACGTTGCTGAGAAGGAGAGGAAAAAGAAGCTCATTGATGGAGGTCTTGAAATCAGTCGTTTAGTAAATATAACTTTAGTTGACCGTGAGGGAAATGCTGTCATACGCCGTCATTTAGAAAGTCTTCCTTTGCAATCTTTTGATTCGATCTTAATTTTGGCTGATGAGTCTGTGGAAGACTCAGCAATTCAAGCTGATTCAAGATCTCTTGCCACATTGCTTTTGATTCGTGATATTCAGGCTAAGCGTCTCCCTATGGTAACACATGTTGATAGAGGAAGCTTTTCACAAAGTTCATGGATAGGGGAAATGCAGCAAGCTTCAGATAAATCAGTTATAATTAGTGAAATTCTGGACCCAAGGACTAAAAATTTATTATCCATGTCAAAGATCAGCGACTATGTGCTATCGAATGAGCTTGTCAGCATGGCACTGGCTATGGTAGCAGAGGATCGGCAAATAAATGATGTATTGGAAGAGCTGTTTGCAGAGGAGGGTAACGAATTGCAAATAAGGCAAGGAGATCTGTATCTCCATGAAGGTGAGGAGTTAAGCTTCTATGAAGTACTCTTGCGCGCCCGACAAAGGAGAGAGGTCATGATTGGTTATCGCTTAGCTGATGCTGAGAGAGCTGTCATCAACCCCCCTGCTAAAAGTGAGAGACGGAGGTGGTCGGTGAAGGATGTATTTGTGGTGATTGCAGAGAAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Castor_Poll_mid

Protein Analysis

884

Amino Acids

98.67

Weight (kDa)

5.2

Isoelectric Point (pI)

57.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Castor_Poll_mid PF06241 497 - 594 1.2e-42 Castor and Pollux, part of voltage-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2279
AasI GACNNNNNNGTC 2 cut(s) 23, 433
AccI GTMKAC 1 cut(s) 1810
AccII CGCG 3 cut(s) 183, 280, 2517
AccIII TCCGGA 1 cut(s) 371
AciI CCGC 9 cut(s) 62, 155, 204, 238, 265, 275, 278, 860, 1867
AclWI GGATC 2 cut(s) 1108, 2400
AcoI YGGCCR 1 cut(s) 1598
AcsI RAATTY 2 cut(s) 2289, 2311
AcuI CTGAAG 3 cut(s) 726, 1341, 2247
AcvI CACGTG 1 cut(s) 1301
AcyI GRCGYC 2 cut(s) 173, 309
AfaI GTAC 7 cut(s) 50, 449, 662, 737, 1632, 1743, 2508
AfeI AGCGCT 1 cut(s) 612
AfiI CCNNNNNNNGG 8 cut(s) 161, 264, 265, 305, 649, 1279, 1929, 2526
AflIII ACRYGT 1 cut(s) 2206
AjuI GAANNNNNNNTTGG 4 cut(s) 1182, 1214, 1545, 1577
Alw21I GWGCWC 2 cut(s) 552, 1929
Alw26I GTCTC 7 cut(s) 36, 625, 679, 1167, 1294, 2195, 2596
AlwI GGATC 2 cut(s) 1108, 2400
AlwNI CAGNNNCTG 1 cut(s) 45
Ama87I CYCGRG 2 cut(s) 676, 1915
Aor13HI TCCGGA 1 cut(s) 371
Aor51HI AGCGCT 1 cut(s) 612
AoxI GGCC 4 cut(s) 233, 319, 1598, 1911
ApeKI GCWGC 4 cut(s) 77, 1528, 1781, 2254
ApoI RAATTY 2 cut(s) 2289, 2311
Asp700I GAANNNNTTC 2 cut(s) 2064, 2224
AspLEI GCGC 4 cut(s) 185, 613, 2517, 2519
AspS9I GGNCC 4 cut(s) 164, 234, 1912, 2296
AsuC2I CCSGG 4 cut(s) 1533, 1706, 1916, 1917
AsuHPI GGTGA 5 cut(s) 51, 681, 2495, 2629, 2647
AsuNHI GCTAGC 1 cut(s) 730
AvaI CYCGRG 2 cut(s) 676, 1915
AvaII GGWCC 2 cut(s) 164, 2296
BalI TGGCCA 1 cut(s) 1600
BanII GRGCYC 3 cut(s) 552, 1256, 1929
BarI GAAGNNNNNNTAC 2 cut(s) 440, 472
BbrPI CACGTG 1 cut(s) 1301
BbsI GAAGAC 3 cut(s) 1329, 2057, 2124
Bbv12I GWGCWC 2 cut(s) 552, 1929
BbvCI CCTCAGC 1 cut(s) 1397
BbvI GCAGC 4 cut(s) 89, 1540, 1793, 2266
BccI CCATC 4 cut(s) 133, 1590, 1604, 1973
BceAI ACGGC 4 cut(s) 160, 170, 334, 2034
BcgI CGANNNNNNTGC 2 cut(s) 1754, 1788
BciVI GTATCC 1 cut(s) 1842
BclI TGATCA 2 cut(s) 1336, 1635
BcnI CCSGG 4 cut(s) 1533, 1706, 1916, 1917
BcoDI GTCTC 7 cut(s) 36, 625, 679, 1167, 1294, 2195, 2596
BfaI CTAG 3 cut(s) 731, 1524, 1538
BfmI CTRYAG 2 cut(s) 1779, 1839
BfoI RGCGCY 1 cut(s) 614
BfuI GTATCC 1 cut(s) 1842
BglI GCCNNNNNGGC 1 cut(s) 1910
BglII AGATCT 4 cut(s) 1465, 1545, 2144, 2464
BisI GCNGC 8 cut(s) 78, 155, 275, 278, 1529, 1782, 1868, 2255
BlpI GCTNAGC 4 cut(s) 856, 1167, 2184, 2554
BlsI GCNGC 8 cut(s) 79, 156, 276, 279, 1530, 1783, 1869, 2256
BmcAI AGTACT 2 cut(s) 1743, 2508
Bme1390I CCNGG 4 cut(s) 1533, 1706, 1916, 1917
Bme18I GGWCC 2 cut(s) 164, 2296
BmeT110I CYCGRG 2 cut(s) 676, 1915
BmgT120I GGNCC 4 cut(s) 164, 234, 1912, 2296
BmiI GGNNCC 5 cut(s) 236, 1253, 1914, 1921, 2298
BmrFI CCNGG 4 cut(s) 1533, 1706, 1916, 1917
BmsI GCATC 8 cut(s) 957, 975, 994, 1336, 1639, 1685, 1891, 2551
BmtI GCTAGC 1 cut(s) 734
BpiI GAAGAC 3 cut(s) 1329, 2057, 2124
BplI GAGNNNNNCTC 2 cut(s) 686, 718
BpmI CTGGAG 3 cut(s) 19, 181, 590
Bpu10I CCTNAGC 1 cut(s) 1397
Bpu1102I GCTNAGC 4 cut(s) 856, 1167, 2184, 2554
BpuMI CCSGG 4 cut(s) 1533, 1706, 1916, 1917
BsaAI YACGTR 1 cut(s) 1301
BsaBI GATNNNNATC 2 cut(s) 579, 2469
BsaHI GRCGYC 2 cut(s) 173, 309
BsaI GGTCTC 1 cut(s) 625
BsaJI CCNNGG 5 cut(s) 1293, 1561, 1914, 1915, 2300
BsaWI WCCGGW 4 cut(s) 371, 390, 691, 1379
Bsc4I CCNNNNNNNGG 8 cut(s) 161, 264, 265, 305, 649, 1279, 1929, 2526
Bse1I ACTGG 2 cut(s) 50, 2379
Bse3DI GCAATG 3 cut(s) 987, 1784, 2156
Bse8I GATNNNNATC 2 cut(s) 579, 2469
BseAI TCCGGA 1 cut(s) 371
BseDI CCNNGG 5 cut(s) 1293, 1561, 1914, 1915, 2300
BseJI GATNNNNATC 2 cut(s) 579, 2469
BseLI CCNNNNNNNGG 8 cut(s) 161, 264, 265, 305, 649, 1279, 1929, 2526
BseMI GCAATG 3 cut(s) 987, 1784, 2156
BseMII CTCAG 7 cut(s) 33, 164, 1158, 1388, 1941, 2136, 2556
BseNI ACTGG 2 cut(s) 50, 2379
BsePI GCGCGC 1 cut(s) 2515
BseRI GAGGAG 2 cut(s) 2447, 2501
BseXI GCAGC 4 cut(s) 89, 1540, 1793, 2266
Bsh1236I CGCG 3 cut(s) 183, 280, 2517
Bsh1285I CGRYCG 1 cut(s) 338
BshFI GGCC 4 cut(s) 235, 321, 1600, 1913
BsiEI CGRYCG 1 cut(s) 338
BsiHKAI GWGCWC 2 cut(s) 552, 1929
BsiHKCI CYCGRG 2 cut(s) 676, 1915
BslFI GGGAC 3 cut(s) 11, 296, 1416
BslI CCNNNNNNNGG 8 cut(s) 161, 264, 265, 305, 649, 1279, 1929, 2526
BsmAI GTCTC 7 cut(s) 36, 625, 679, 1167, 1294, 2195, 2596
BsmBI CGTCTC 2 cut(s) 2195, 2596
BsmFI GGGAC 3 cut(s) 11, 296, 1416
BsnI GGCC 4 cut(s) 235, 321, 1600, 1913
Bso31I GGTCTC 1 cut(s) 625
BsoBI CYCGRG 2 cut(s) 676, 1915
Bsp1286I GDGCHC 3 cut(s) 552, 1256, 1929
Bsp13I TCCGGA 1 cut(s) 371
Bsp1720I GCTNAGC 4 cut(s) 856, 1167, 2184, 2554
BspACI CCGC 9 cut(s) 62, 155, 204, 238, 265, 275, 278, 860, 1867
BspANI GGCC 4 cut(s) 235, 321, 1600, 1913
BspCNI CTCAG 7 cut(s) 32, 163, 1159, 1389, 1942, 2135, 2557
BspEI TCCGGA 1 cut(s) 371
BspFNI CGCG 3 cut(s) 183, 280, 2517
BspHI TCATGA 2 cut(s) 1489, 2538
BspLI GGNNCC 5 cut(s) 236, 1253, 1914, 1921, 2298
BspMAI CTGCAG 2 cut(s) 1783, 1843
BspOI GCTAGC 1 cut(s) 734
BspPI GGATC 2 cut(s) 1108, 2400
BspQI GCTCTTC 1 cut(s) 2412
BspTNI GGTCTC 1 cut(s) 625
BsrDI GCAATG 3 cut(s) 987, 1784, 2156
BsrI ACTGG 2 cut(s) 50, 2379
BssECI CCNNGG 5 cut(s) 1293, 1561, 1914, 1915, 2300
BssHII GCGCGC 1 cut(s) 2515
BssNI GRCGYC 2 cut(s) 173, 309
BssT1I CCWWGG 3 cut(s) 1293, 1561, 2300
Bst4CI ACNGT 8 cut(s) 430, 687, 740, 804, 1366, 1480, 1605, 2027
Bst6I CTCTTC 1 cut(s) 2412
BstACI GRCGYC 2 cut(s) 173, 309
BstBAI YACGTR 1 cut(s) 1301
BstC8I GCNNGC 4 cut(s) 732, 1616, 2259, 2517
BstEII GGTNACC 1 cut(s) 687
BstENI CCTNNNNNAGG 1 cut(s) 1277
BstFNI CGCG 3 cut(s) 183, 280, 2517
BstH2I RGCGCY 1 cut(s) 614
BstHHI GCGC 4 cut(s) 185, 613, 2517, 2519
BstMAI GTCTC 7 cut(s) 36, 625, 679, 1167, 1294, 2195, 2596
BstMCI CGRYCG 1 cut(s) 338
BstMWI GCNNNNNNNGC 7 cut(s) 271, 1781, 1910, 2132, 2384, 2455, 2570
BstNSI RCATGY 2 cut(s) 1618, 2210
BstPI GGTNACC 1 cut(s) 687
BstSCI CCNGG 4 cut(s) 1531, 1704, 1914, 1915
BstSFI CTRYAG 2 cut(s) 1779, 1839
BstUI CGCG 3 cut(s) 183, 280, 2517
BstV1I GCAGC 4 cut(s) 89, 1540, 1793, 2266
BstV2I GAAGAC 3 cut(s) 1329, 2057, 2124
BstX2I RGATCY 4 cut(s) 1465, 1545, 2144, 2464
BstYI RGATCY 4 cut(s) 1465, 1545, 2144, 2464
BsuI GTATCC 1 cut(s) 1842
BsuRI GGCC 4 cut(s) 235, 321, 1600, 1913
BtgZI GCGATG 1 cut(s) 1749
BtsIMutI CAGTG 4 cut(s) 447, 779, 800, 2372
Cac8I GCNNGC 4 cut(s) 732, 1616, 2259, 2517
CaiI CAGNNNCTG 1 cut(s) 45
CciI TCATGA 2 cut(s) 1489, 2538
CfoI GCGC 4 cut(s) 185, 613, 2517, 2519
Cfr13I GGNCC 4 cut(s) 164, 234, 1912, 2296
Cfr9I CCCGGG 1 cut(s) 1915
CseI GACGC 3 cut(s) 181, 298, 2177
Csp6I GTAC 7 cut(s) 49, 448, 661, 736, 1631, 1742, 2507
CspCI CAANNNNNGTGG 2 cut(s) 768, 803
CviQI GTAC 7 cut(s) 49, 448, 661, 736, 1631, 1742, 2507
DrdI GACNNNNNNGTC 2 cut(s) 23, 433
DseDI GACNNNNNNGTC 2 cut(s) 23, 433
EaeI YGGCCR 1 cut(s) 1598
Eam1104I CTCTTC 1 cut(s) 2412
EarI CTCTTC 1 cut(s) 2412
EciI GGCGGA 1 cut(s) 193
Ecl136II GAGCTC 2 cut(s) 550, 1927
Eco130I CCWWGG 3 cut(s) 1293, 1561, 2300
Eco24I GRGCYC 3 cut(s) 552, 1256, 1929
Eco31I GGTCTC 1 cut(s) 625
Eco32I GATATC 1 cut(s) 1558
Eco47I GGWCC 2 cut(s) 164, 2296
Eco47III AGCGCT 1 cut(s) 612
Eco53kI GAGCTC 2 cut(s) 550, 1927
Eco57I CTGAAG 3 cut(s) 726, 1341, 2247
Eco72I CACGTG 1 cut(s) 1301
Eco88I CYCGRG 2 cut(s) 676, 1915
Eco91I GGTNACC 1 cut(s) 687
EcoICRI GAGCTC 2 cut(s) 550, 1927
EcoNI CCTNNNNNAGG 1 cut(s) 1277
EcoO109I RGGNCCY 1 cut(s) 234
EcoO65I GGTNACC 1 cut(s) 687
EcoRV GATATC 1 cut(s) 1558
EcoT14I CCWWGG 3 cut(s) 1293, 1561, 2300
EcoT22I ATGCAT 1 cut(s) 1620
EcoT38I GRGCYC 3 cut(s) 552, 1256, 1929
ErhI CCWWGG 3 cut(s) 1293, 1561, 2300
Esp3I CGTCTC 2 cut(s) 2195, 2596
FalI AAGNNNNNCTT 4 cut(s) 1545, 1577, 2053, 2085
FaqI GGGAC 3 cut(s) 11, 296, 1416
FauI CCCGC 2 cut(s) 245, 258
FbaI TGATCA 2 cut(s) 1336, 1635
FblI GTMKAC 1 cut(s) 1810
Fnu4HI GCNGC 8 cut(s) 78, 155, 275, 278, 1529, 1782, 1868, 2255
FriOI GRGCYC 3 cut(s) 552, 1256, 1929
Fsp4HI GCNGC 8 cut(s) 78, 155, 275, 278, 1529, 1782, 1868, 2255
FspBI CTAG 3 cut(s) 731, 1524, 1538
GlaI GCGC 4 cut(s) 184, 612, 2516, 2518
GluI GCNGC 8 cut(s) 78, 155, 275, 278, 1529, 1782, 1868, 2255
GsuI CTGGAG 3 cut(s) 19, 181, 590
HaeII RGCGCY 1 cut(s) 614
HaeIII GGCC 4 cut(s) 235, 321, 1600, 1913
HgaI GACGC 3 cut(s) 181, 298, 2177
HhaI GCGC 4 cut(s) 185, 613, 2517, 2519
Hin1I GRCGYC 2 cut(s) 173, 309
Hin6I GCGC 4 cut(s) 183, 611, 2515, 2517
HinP1I GCGC 4 cut(s) 183, 611, 2515, 2517
HincII GTYRAC 3 cut(s) 250, 292, 2023
HindII GTYRAC 3 cut(s) 250, 292, 2023
HindIII AAGCTT 4 cut(s) 503, 2221, 2259, 2494
HphI GGTGA 5 cut(s) 51, 681, 2495, 2629, 2647
Hpy166II GTNNAC 8 cut(s) 250, 292, 448, 464, 763, 882, 1811, 2023
Hpy8I GTNNAC 8 cut(s) 250, 292, 448, 464, 763, 882, 1811, 2023
Hpy99I CGWCG 3 cut(s) 175, 264, 320
HpyAV CCTTC 7 cut(s) 39, 76, 1724, 1948, 2474, 2614, 2641
HpyCH4III ACNGT 8 cut(s) 430, 687, 740, 804, 1366, 1480, 1605, 2027
HpyCH4IV ACGT 3 cut(s) 884, 1300, 1944
HpyF10VI GCNNNNNNNGC 7 cut(s) 271, 1781, 1910, 2132, 2384, 2455, 2570
HpySE526I ACGT 3 cut(s) 884, 1300, 1944
Hsp92I GRCGYC 2 cut(s) 173, 309
HspAI GCGC 4 cut(s) 183, 611, 2515, 2517
Kpn2I TCCGGA 1 cut(s) 371
Ksp22I TGATCA 2 cut(s) 1336, 1635
LguI GCTCTTC 1 cut(s) 2412
LmnI GCTCC 6 cut(s) 206, 547, 1057, 1086, 1251, 1780
Lsp1109I GCAGC 4 cut(s) 89, 1540, 1793, 2266
LweI GCATC 8 cut(s) 957, 975, 994, 1336, 1639, 1685, 1891, 2551
MaeI CTAG 3 cut(s) 731, 1524, 1538
MaeII ACGT 3 cut(s) 884, 1300, 1944
MaeIII GTNAC 4 cut(s) 687, 804, 2200, 2438
MflI RGATCY 4 cut(s) 1465, 1545, 2144, 2464
MhlI GDGCHC 3 cut(s) 552, 1256, 1929
MlsI TGGCCA 1 cut(s) 1600
MluNI TGGCCA 1 cut(s) 1600
MlyI GAGTC 9 cut(s) 5, 11, 369, 908, 1019, 1674, 1706, 2114, 2117
MmeI TCCRAC 4 cut(s) 39, 1064, 1587, 1878
Mox20I TGGCCA 1 cut(s) 1600
Mph1103I ATGCAT 1 cut(s) 1620
MroI TCCGGA 1 cut(s) 371
MroXI GAANNNNTTC 2 cut(s) 2064, 2224
MscI TGGCCA 1 cut(s) 1600
MseI TTAA 6 cut(s) 599, 1073, 1370, 1449, 2093, 2492
MslI CAYNNNNRTG 1 cut(s) 2481
Msp20I TGGCCA 1 cut(s) 1600
MspR9I CCNGG 4 cut(s) 1533, 1706, 1916, 1917
MvnI CGCG 3 cut(s) 183, 280, 2517
MwoI GCNNNNNNNGC 7 cut(s) 271, 1781, 1910, 2132, 2384, 2455, 2570
NciI CCSGG 4 cut(s) 1533, 1706, 1916, 1917
NheI GCTAGC 1 cut(s) 730
NlaIV GGNNCC 5 cut(s) 236, 1253, 1914, 1921, 2298
NmeAIII GCCGAG 1 cut(s) 357
NmuCI GTSAC 1 cut(s) 687
NsiI ATGCAT 1 cut(s) 1620
NspI RCATGY 2 cut(s) 1618, 2210
PaeI GCATGC 1 cut(s) 1618
PaeR7I CTCGAG 1 cut(s) 676
PagI TCATGA 2 cut(s) 1489, 2538
PauI GCGCGC 1 cut(s) 2515
PciI ACATGT 1 cut(s) 2206
PciSI GCTCTTC 1 cut(s) 2412
PcsI WCGNNNNNNNCGW 1 cut(s) 1086
PdmI GAANNNNTTC 2 cut(s) 2064, 2224
PfeI GAWTC 9 cut(s) 105, 146, 539, 713, 896, 1513, 2084, 2138, 2167
PflFI GACNNNGTC 2 cut(s) 356, 671
PkrI GCNGC 8 cut(s) 79, 156, 276, 279, 1530, 1783, 1869, 2256
Ple19I CGATCG 1 cut(s) 338
PleI GAGTC 9 cut(s) 5, 11, 369, 908, 1019, 1673, 1706, 2114, 2116
PmaCI CACGTG 1 cut(s) 1301
PmlI CACGTG 1 cut(s) 1301
PpsI GAGTC 9 cut(s) 5, 11, 369, 908, 1019, 1673, 1706, 2114, 2116
Ppu21I YACGTR 1 cut(s) 1301
PscI ACATGT 1 cut(s) 2206
PsiI TTATAA 1 cut(s) 2279
Psp124BI GAGCTC 2 cut(s) 552, 1929
PspCI CACGTG 1 cut(s) 1301
PspEI GGTNACC 1 cut(s) 687
PspN4I GGNNCC 5 cut(s) 236, 1253, 1914, 1921, 2298
PspPI GGNCC 4 cut(s) 164, 234, 1912, 2296
PstI CTGCAG 2 cut(s) 1783, 1843
PstNI CAGNNNCTG 1 cut(s) 45
PsuI RGATCY 4 cut(s) 1465, 1545, 2144, 2464
PsyI GACNNNGTC 2 cut(s) 356, 671
PteI GCGCGC 1 cut(s) 2515
PvuI CGATCG 1 cut(s) 338
RsaI GTAC 7 cut(s) 50, 449, 662, 737, 1632, 1743, 2508
RsaNI GTAC 7 cut(s) 49, 448, 661, 736, 1631, 1742, 2507
RseI CAYNNNNRTG 1 cut(s) 2481
SacI GAGCTC 2 cut(s) 552, 1929
SapI GCTCTTC 1 cut(s) 2412
SaqAI TTAA 6 cut(s) 599, 1073, 1370, 1449, 2093, 2492
SatI GCNGC 8 cut(s) 78, 155, 275, 278, 1529, 1782, 1868, 2255
Sau96I GGNCC 4 cut(s) 164, 234, 1912, 2296
ScaI AGTACT 2 cut(s) 1743, 2508
SchI GAGTC 9 cut(s) 5, 11, 369, 908, 1019, 1674, 1706, 2114, 2117
ScrFI CCNGG 4 cut(s) 1533, 1706, 1916, 1917
SduI GDGCHC 3 cut(s) 552, 1256, 1929
SfaNI GCATC 8 cut(s) 957, 975, 994, 1336, 1639, 1685, 1891, 2551
SfcI CTRYAG 2 cut(s) 1779, 1839
Sfr274I CTCGAG 1 cut(s) 676
SinI GGWCC 2 cut(s) 164, 2296
SlaI CTCGAG 1 cut(s) 676
SmaI CCCGGG 1 cut(s) 1917
SmiMI CAYNNNNRTG 1 cut(s) 2481
SmlI CTYRAG 1 cut(s) 676
SmoI CTYRAG 1 cut(s) 676
SphI GCATGC 1 cut(s) 1618
SsiI CCGC 9 cut(s) 62, 155, 204, 238, 265, 275, 278, 860, 1867
SspMI CTAG 3 cut(s) 731, 1524, 1538
SstI GAGCTC 2 cut(s) 552, 1929
StyD4I CCNGG 4 cut(s) 1531, 1704, 1914, 1915
StyI CCWWGG 3 cut(s) 1293, 1561, 2300
TaaI ACNGT 8 cut(s) 430, 687, 740, 804, 1366, 1480, 1605, 2027
TaiI ACGT 3 cut(s) 887, 1303, 1947
TaqI TCGA 5 cut(s) 9, 677, 744, 2087, 2351
TatI WGTACW 4 cut(s) 660, 1630, 1741, 2506
TauI GCSGC 4 cut(s) 157, 277, 280, 1870
TfiI GAWTC 9 cut(s) 105, 146, 539, 713, 896, 1513, 2084, 2138, 2167
Tru1I TTAA 6 cut(s) 599, 1073, 1370, 1449, 2093, 2492
Tru9I TTAA 6 cut(s) 599, 1073, 1370, 1449, 2093, 2492
TscAI CASTG 4 cut(s) 447, 786, 807, 2379
TseFI GTSAC 1 cut(s) 687
TseI GCWGC 4 cut(s) 77, 1528, 1781, 2254
Tsp45I GTSAC 1 cut(s) 687
TspDTI ATGAA 5 cut(s) 58, 1752, 2226, 2493, 2517
TspGWI ACGGA 2 cut(s) 1341, 2620
TspMI CCCGGG 1 cut(s) 1915
TspRI CASTG 4 cut(s) 447, 786, 807, 2379
Tth111I GACNNNGTC 2 cut(s) 356, 671
VpaK11BI GGWCC 2 cut(s) 164, 2296
XagI CCTNNNNNAGG 1 cut(s) 1277
XapI RAATTY 2 cut(s) 2289, 2311
XceI RCATGY 2 cut(s) 1618, 2210
XhoI CTCGAG 1 cut(s) 676
XmaI CCCGGG 1 cut(s) 1915
XmiI GTMKAC 1 cut(s) 1810
XmnI GAANNNNTTC 2 cut(s) 2064, 2224
XspI CTAG 3 cut(s) 731, 1524, 1538
ZrmI AGTACT 2 cut(s) 1743, 2508
Zsp2I ATGCAT 1 cut(s) 1620
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.