Rh7CG355500

Ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0001000
Physical Location & Seq
Reverse (-)
40927782 .. 40939063
11282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG355500.1

Sequence Viewer

Length: 2523 bp
ATGCTTGACCGCAATGAAGAGTCGTCGAAGCCCGACAAGCCGGAAAGCCCACCGCAGCTGAAGAAATCACGGACTATAAGCGGCGACGATGCAACCCCAATCTTCCCGGGCCCACTGTTCCCAGCCGTTCGACGCATCTCCAGCATTCCTTCCTCCCACAACTGGCCCCGGCCCGATCGCCACGCGTCGACTACTGCGGCGGACCACCGCGACTTCTCTGACCGTGACTGGGTCTTTCCGTCCTTTGTGCTTCCACAGAATCCTTCCAAACGAAGCAGCAAGAAACACGCCTCCGATGCATCCACCGCTTCTCAGCTTGCGGGGGCCACCGAAGCTGCTGCTGAGAGGAAGAAAATTAAGTTGGTGCCCCAGCCCGGACGTGAAGCACCTTCAACGAGCGAGTTGACTCGCCCGAGTTCCAGGACCCGGGCTCGAGATTTGAAACCTTCTTTGTTGCTATTATTTTTGCTCACTTTCATTTGTATATTATCTGTACCTTATGCAGTTTATCTGCGGCAAAGAATTGTAAAACTTGAGGAATGTTGTATCAGTAAAAATGCTGGTACAGATGACAATATTGAGGCTTCACTGTCTGAGAAGAATCCAACCATTTTGCGTTTTCCTAATGCCGAAAGAAGAACTCTTGTTTTGTATTCTGTGGTCTTTACACTGGCCATGCCATTTGTGCTGTACAAACATCTTGATTATTTTTCCCATTTGACAATCCTCTCAAAAAACAAGAATAACAAAAAGGGGGTTCCCTTGAAGAAGAGGATAGCCTATATAGTGGATGTGTGCTTTTCTGTATATCCATATGCTAAACTACTTGCACTTCTCTTGGCAACAATATTACTAATAGGGTTTGGTGGACTGGCATTGTATGCAGTCAATAAAAATAGCTTTGCAGAAGCTCTTTGGCTTTCATGGACTTTTGTTGCTGATTCTGGAAACCATGCTGATACAGAGGGCATTGGGCCAAGGATAGTTTCTGTCTCTATTAGTTCAGGAGGCATGCTTATATTTGCGATGATGCTTGGGCTTGTTTCTGATGCTATATCAGAGAAGGTTGACTCACTAAGGAAAGGGAAGAGTGAAGTCATTGAAAGGAACCACATACTCGTTCTTGGATGGAGTGATAAACTGGGTTCGCTTCTCAAGCAGCTTGCTATAGCAAACAAGAGTGTTGGTGGTGGTGTTGTTGTTGTACTTGCTGAAAGAGACAAGGAGGAAATGGAGATAGATATTGCAAAACTTGAATTTGATTTCATGGGTACGTCTGTTATATGCAGAAGTGGCAGTCCTCTTATACTTGCTGACCTAAAGAAGGTCTCAGTTTCGAAGGCGCGTGCTATCATTGTATTGGCATCTGATGAAAATGCAGATCAGAGTGATGCACGTGCTCTGAGGGTTGTTCTAAGCCTCACTGGGGTGAAAGAGGGTTTGAGGGGACATGTTGTTGTAGAGATGAGTGACCTGGATAATGAGCCTCTTGTGAAGCTTGTTGGAGGAGAACTCATTGAAACAGTAGTAGCACATGATGTGATTGGGCGGTTAATGATACAGTGTGCTCTACAGCCTGGTCTTGCGCAGATATGGGAGGATATATTGGGATTTGAGAATGCTGAGTTTTACATCAAAAGGTGGCCTCAGTTGGATGGTCTTCGTTTCGAAAATGTGCTCATTTCATTTCCTGATGCAATTCCCTGTGGAATCAAGGTTGCCGCAGATGGTGGAAAGATAAATTTAAATCCCGATGATAAATATGTTATGAAAGAAGGGGATGAAGTTCTTGTTATAGCTGAAGATGACGACACTTATGCTCCATCTGCCTTTCCAGAGGTATGTGCTGGCCTTTGTCCAAACACTGTTGAACCTCCCAAATACCCTGAGAAGATATTGTTCTGTGGCTGGCGTCGTGACATAGACGATATGATACTGGTTCTAGAGGCATTCTTGCCTCTAGGTTCAGAACTTTGGATGTTCAATGAGGTTCCAGAAAAAGAAAGAGAGAAGAAACTTACAGACGGTGGACTTGATATTTCGCGATTGGAGAACATAAAACTCGTTCACAAAGAAGGAAATGCTGTGATAAGACGGCATCTAGAGAGTCTTCCCTTGGAGACTTTTGATTCTATATTAATTCTTGCAGATGAATCACTAGAGGACTCTGTTGTGCATTCTGACTCGAGATCCCTTGCCACTCTTCTCCTTATACGAGATATACAGTCAAAGCGTCTTCCTTACAAAGAAACAAAGTCAACTTCCTTATCTGCATTTTCGCATAGCTTTTGGATTCGTGAAATGCAGCAAGCTTCAGACAAATCGATAATTATAAGTGAAATTCTGGATTCTAGGACTAGAAACCTGGTCTCTGTTTCCAGAATCAGTGATTATGTGCTGTCAAATGAACTGGTTAGTATGGCACTGGCAATGGTGGCTGAAGATAAGCAAATCAATCGCGTTCTTGAGGAGCTATTTGCGGAGGAGGTACTCTCCAGTCTTTCAGGAATTTATGTTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Castor_Poll_mid

Protein Analysis

840

Amino Acids

93.04

Weight (kDa)

5.73

Isoelectric Point (pI)

47.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Slo-like_RCK PF22614 369 - 473 2.2e-07 Calcium-activated potassium channel slowpoke-like RCK domain
Castor_Poll_mid PF06241 512 - 609 1.9e-42 Castor and Pollux, part of voltage-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2333
Acc16I TGCGCA 1 cut(s) 1587
AccB1I GGYRCC 1 cut(s) 364
AccI GTMKAC 1 cut(s) 188
AccII CGCG 5 cut(s) 185, 210, 1345, 2044, 2460
AclWI GGATC 1 cut(s) 2184
AcoI YGGCCR 1 cut(s) 672
AcsI RAATTY 4 cut(s) 1256, 1741, 2340, 2508
AcuI CTGAAG 4 cut(s) 80, 1821, 2298, 2460
AcvI CACGTG 1 cut(s) 1397
AcyI GRCGYC 1 cut(s) 1912
AfaI GTAC 6 cut(s) 495, 565, 692, 1206, 1273, 2490
AfiI CCNNNNNNNGG 6 cut(s) 162, 229, 374, 426, 1324, 1426
AflIII ACRYGT 2 cut(s) 183, 1450
AgsI TTSAA 8 cut(s) 393, 442, 766, 1103, 1256, 1520, 1871, 1984
AjiI CACGTC 1 cut(s) 380
AjnI CCWGG 4 cut(s) 419, 1473, 1576, 2364
AjuI GAANNNNNNNTTGG 4 cut(s) 344, 376, 970, 1002
AleI CACNNNNGTG 1 cut(s) 1427
Alw21I GWGCWC 3 cut(s) 1402, 1570, 1680
Alw26I GTCTC 5 cut(s) 997, 1212, 1333, 2114, 2374
AlwI GGATC 1 cut(s) 2184
Ama87I CYCGRG 5 cut(s) 106, 412, 426, 432, 2185
AoxI GGCC 8 cut(s) 109, 164, 170, 324, 672, 974, 1643, 1849
ApaI GGGCCC 1 cut(s) 113
ApeKI GCWGC 6 cut(s) 55, 276, 335, 338, 1159, 2305
ApoI RAATTY 4 cut(s) 1256, 1741, 2340, 2508
AseI ATTAAT 1 cut(s) 2138
AspLEI GCGC 2 cut(s) 1345, 1588
AspS9I GGNCC 8 cut(s) 109, 110, 165, 171, 202, 324, 423, 974
AsuC2I CCSGG 6 cut(s) 107, 108, 169, 375, 427, 428
AsuHPI GGTGA 1 cut(s) 1441
AsuII TTCGAA 2 cut(s) 1337, 1668
AvaI CYCGRG 5 cut(s) 106, 412, 426, 432, 2185
AvaII GGWCC 2 cut(s) 202, 423
BaeGI GKGCMC 2 cut(s) 113, 369
BaeI ACNNNNGTAYC 2 cut(s) 1263, 1296
BalI TGGCCA 1 cut(s) 674
BanI GGYRCC 1 cut(s) 364
BanII GRGCYC 2 cut(s) 113, 433
BbrPI CACGTG 1 cut(s) 1397
BbsI GAAGAC 3 cut(s) 1652, 2102, 2228
Bbv12I GWGCWC 3 cut(s) 1402, 1570, 1680
BbvI GCAGC 6 cut(s) 67, 288, 322, 325, 1171, 2317
BccI CCATC 4 cut(s) 1122, 1649, 1721, 1831
BceAI ACGGC 2 cut(s) 110, 2111
BcgI CGANNNNNNTGC 6 cut(s) 320, 354, 1799, 1833, 2438, 2472
BciT130I CCWGG 4 cut(s) 421, 1475, 1578, 2366
BcnI CCSGG 6 cut(s) 107, 108, 169, 375, 427, 428
BcoDI GTCTC 5 cut(s) 997, 1212, 1333, 2114, 2374
BfaI CTAG 6 cut(s) 1943, 1961, 2102, 2159, 2352, 2358
BfmI CTRYAG 2 cut(s) 1167, 1571
Bme18I GGWCC 2 cut(s) 202, 423
BmeT110I CYCGRG 5 cut(s) 106, 412, 426, 432, 2185
BmgBI CACGTC 1 cut(s) 380
BmgT120I GGNCC 8 cut(s) 109, 110, 165, 171, 202, 324, 423, 974
BmiI GGNNCC 8 cut(s) 111, 167, 325, 366, 425, 759, 1109, 1992
BmrI ACTGGG 3 cut(s) 238, 1151, 1434
BmuI ACTGGG 3 cut(s) 238, 1151, 1434
BpiI GAAGAC 3 cut(s) 1652, 2102, 2228
BplI GAGNNNNNCTC 4 cut(s) 1497, 1529, 2477, 2509
BpmI CTGGAG 2 cut(s) 124, 2479
Bpu14I TTCGAA 2 cut(s) 1337, 1668
BpuEI CTTGAG 3 cut(s) 554, 1139, 2486
BpuMI CCSGG 6 cut(s) 107, 108, 169, 375, 427, 428
Bsa29I ATCGAT 1 cut(s) 2324
BsaAI YACGTR 1 cut(s) 1397
BsaHI GRCGYC 1 cut(s) 1912
BsaI GGTCTC 2 cut(s) 1333, 2374
BsaJI CCNNGG 5 cut(s) 106, 167, 426, 977, 2115
BsaXI ACNNNNNCTCC 2 cut(s) 1804, 1834
Bsc4I CCNNNNNNNGG 6 cut(s) 162, 229, 374, 426, 1324, 1426
Bse3DI GCAATG 2 cut(s) 19, 2436
BseBI CCWGG 4 cut(s) 421, 1475, 1578, 2366
BseCI ATCGAT 1 cut(s) 2324
BseDI CCNNGG 5 cut(s) 106, 167, 426, 977, 2115
BseGI GGATG 6 cut(s) 299, 796, 1133, 1660, 1786, 1983
BseLI CCNNNNNNNGG 6 cut(s) 162, 229, 374, 426, 1324, 1426
BseMI GCAATG 2 cut(s) 19, 2436
BseMII CTCAG 8 cut(s) 326, 333, 585, 1344, 1394, 1614, 1661, 1878
BseRI GAGGAG 3 cut(s) 1521, 2483, 2498
BseSI GKGCMC 2 cut(s) 113, 369
BseXI GCAGC 6 cut(s) 67, 288, 322, 325, 1171, 2317
BseYI CCCAGC 2 cut(s) 121, 369
Bsh1236I CGCG 5 cut(s) 185, 210, 1345, 2044, 2460
Bsh1285I CGRYCG 1 cut(s) 178
BshFI GGCC 8 cut(s) 111, 166, 172, 326, 674, 976, 1645, 1851
BshNI GGYRCC 1 cut(s) 364
BshVI ATCGAT 1 cut(s) 2324
BsiEI CGRYCG 1 cut(s) 178
BsiHKAI GWGCWC 3 cut(s) 1402, 1570, 1680
BsiHKCI CYCGRG 5 cut(s) 106, 412, 426, 432, 2185
BsiSI CCGG 5 cut(s) 41, 107, 169, 375, 427
BslFI GGGAC 1 cut(s) 1461
BslI CCNNNNNNNGG 6 cut(s) 162, 229, 374, 426, 1324, 1426
BsmAI GTCTC 5 cut(s) 997, 1212, 1333, 2114, 2374
BsmFI GGGAC 1 cut(s) 1461
BsmI GAATGC 4 cut(s) 144, 1624, 1949, 2176
BsnI GGCC 8 cut(s) 111, 166, 172, 326, 674, 976, 1645, 1851
Bso31I GGTCTC 2 cut(s) 1333, 2374
BsoBI CYCGRG 5 cut(s) 106, 412, 426, 432, 2185
Bsp119I TTCGAA 2 cut(s) 1337, 1668
Bsp120I GGGCCC 1 cut(s) 109
Bsp1286I GDGCHC 6 cut(s) 113, 369, 433, 1402, 1570, 1680
Bsp1407I TGTACA 1 cut(s) 690
Bsp143I GATC 3 cut(s) 175, 1381, 2189
Bsp68I TCGCGA 1 cut(s) 2044
BspANI GGCC 8 cut(s) 111, 166, 172, 326, 674, 976, 1645, 1851
BspCNI CTCAG 8 cut(s) 325, 334, 586, 1343, 1395, 1615, 1660, 1879
BspDI ATCGAT 1 cut(s) 2324
BspFNI CGCG 5 cut(s) 185, 210, 1345, 2044, 2460
BspLI GGNNCC 8 cut(s) 111, 167, 325, 366, 425, 759, 1109, 1992
BspPI GGATC 1 cut(s) 2184
BspT104I TTCGAA 2 cut(s) 1337, 1668
BspT107I GGYRCC 1 cut(s) 364
BspTNI GGTCTC 2 cut(s) 1333, 2374
BsrDI GCAATG 2 cut(s) 19, 2436
BsrGI TGTACA 1 cut(s) 690
BssECI CCNNGG 5 cut(s) 106, 167, 426, 977, 2115
BssMI GATC 3 cut(s) 175, 1381, 2189
BssNI GRCGYC 1 cut(s) 1912
BssT1I CCWWGG 2 cut(s) 977, 2115
Bst2UI CCWGG 4 cut(s) 421, 1475, 1578, 2366
Bst4CI ACNGT 8 cut(s) 117, 224, 591, 1525, 1563, 1867, 2027, 2226
Bst6I CTCTTC 4 cut(s) 12, 764, 1082, 2208
BstACI GRCGYC 1 cut(s) 1912
BstAPI GCANNNNNTGC 1 cut(s) 881
BstAUI TGTACA 1 cut(s) 690
BstBAI YACGTR 1 cut(s) 1397
BstBI TTCGAA 2 cut(s) 1337, 1668
BstC8I GCNNGC 7 cut(s) 318, 1013, 1164, 1347, 1849, 1910, 2310
BstENI CCTNNNNNAGG 1 cut(s) 1322
BstF5I GGATG 6 cut(s) 299, 796, 1133, 1660, 1786, 1983
BstFNI CGCG 5 cut(s) 185, 210, 1345, 2044, 2460
BstHHI GCGC 2 cut(s) 1345, 1588
BstKTI GATC 3 cut(s) 178, 1384, 2192
BstMAI GTCTC 5 cut(s) 997, 1212, 1333, 2114, 2374
BstMBI GATC 3 cut(s) 175, 1381, 2189
BstMCI CGRYCG 1 cut(s) 178
BstNI CCWGG 4 cut(s) 421, 1475, 1578, 2366
BstNSI RCATGY 2 cut(s) 1015, 1454
BstSFI CTRYAG 2 cut(s) 1167, 1571
BstSLI GKGCMC 2 cut(s) 113, 369
BstUI CGCG 5 cut(s) 185, 210, 1345, 2044, 2460
BstV1I GCAGC 6 cut(s) 67, 288, 322, 325, 1171, 2317
BstV2I GAAGAC 3 cut(s) 1652, 2102, 2228
BstX2I RGATCY 1 cut(s) 2189
BstYI RGATCY 1 cut(s) 2189
Bsu15I ATCGAT 1 cut(s) 2324
BsuRI GGCC 8 cut(s) 111, 166, 172, 326, 674, 976, 1645, 1851
BsuTUI ATCGAT 1 cut(s) 2324
BtgZI GCGATG 1 cut(s) 1040
BtrI CACGTC 1 cut(s) 380
BtsCI GGATG 6 cut(s) 299, 796, 1133, 1660, 1786, 1983
BtsIMutI CAGTG 8 cut(s) 113, 587, 668, 1422, 1568, 1863, 2392, 2423
BtuMI TCGCGA 1 cut(s) 2044
Cac8I GCNNGC 7 cut(s) 318, 1013, 1164, 1347, 1849, 1910, 2310
CfoI GCGC 2 cut(s) 1345, 1588
Cfr13I GGNCC 8 cut(s) 109, 110, 165, 171, 202, 324, 423, 974
Cfr9I CCCGGG 2 cut(s) 106, 426
ClaI ATCGAT 1 cut(s) 2324
CseI GACGC 4 cut(s) 141, 174, 1901, 2222
CsiI ACCWGGT 1 cut(s) 2364
Csp6I GTAC 6 cut(s) 494, 564, 691, 1205, 1272, 2489
CviAII CATG 7 cut(s) 676, 924, 953, 1012, 1267, 1451, 1535
CviQI GTAC 6 cut(s) 494, 564, 691, 1205, 1272, 2489
DpnI GATC 3 cut(s) 177, 1383, 2191
DpnII GATC 3 cut(s) 175, 1381, 2189
DraI TTTAAA 1 cut(s) 1746
EaeI YGGCCR 1 cut(s) 672
Eam1104I CTCTTC 4 cut(s) 12, 764, 1082, 2208
EarI CTCTTC 4 cut(s) 12, 764, 1082, 2208
EciI GGCGGA 1 cut(s) 215
Eco130I CCWWGG 2 cut(s) 977, 2115
Eco24I GRGCYC 2 cut(s) 113, 433
Eco31I GGTCTC 2 cut(s) 1333, 2374
Eco47I GGWCC 2 cut(s) 202, 423
Eco57I CTGAAG 4 cut(s) 80, 1821, 2298, 2460
Eco72I CACGTG 1 cut(s) 1397
Eco88I CYCGRG 5 cut(s) 106, 412, 426, 432, 2185
EcoNI CCTNNNNNAGG 1 cut(s) 1322
EcoO109I RGGNCCY 1 cut(s) 423
EcoRII CCWGG 4 cut(s) 419, 1473, 1576, 2364
EcoT14I CCWWGG 2 cut(s) 977, 2115
EcoT22I ATGCAT 1 cut(s) 301
EcoT38I GRGCYC 2 cut(s) 113, 433
ErhI CCWWGG 2 cut(s) 977, 2115
FaeI CATG 7 cut(s) 679, 927, 956, 1015, 1270, 1454, 1538
FaqI GGGAC 1 cut(s) 1461
FatI CATG 7 cut(s) 675, 923, 952, 1011, 1266, 1450, 1534
FauI CCCGC 1 cut(s) 313
FauNDI CATATG 1 cut(s) 814
FblI GTMKAC 1 cut(s) 188
FokI GGATG 6 cut(s) 286, 803, 1140, 1667, 1793, 1990
FriOI GRGCYC 2 cut(s) 113, 433
FspBI CTAG 6 cut(s) 1943, 1961, 2102, 2159, 2352, 2358
FspI TGCGCA 1 cut(s) 1587
GlaI GCGC 2 cut(s) 1344, 1587
GsaI CCCAGC 2 cut(s) 125, 373
GsuI CTGGAG 2 cut(s) 124, 2479
HaeIII GGCC 8 cut(s) 111, 166, 172, 326, 674, 976, 1645, 1851
HapII CCGG 5 cut(s) 41, 107, 169, 375, 427
HgaI GACGC 4 cut(s) 141, 174, 1901, 2222
HhaI GCGC 2 cut(s) 1345, 1588
Hin1I GRCGYC 1 cut(s) 1912
Hin1II CATG 7 cut(s) 679, 927, 956, 1015, 1270, 1454, 1538
Hin6I GCGC 2 cut(s) 1343, 1586
HinP1I GCGC 2 cut(s) 1343, 1586
HincII GTYRAC 4 cut(s) 189, 405, 1069, 2259
HindII GTYRAC 4 cut(s) 189, 405, 1069, 2259
HindIII AAGCTT 2 cut(s) 1496, 2310
HpaII CCGG 5 cut(s) 41, 107, 169, 375, 427
HphI GGTGA 1 cut(s) 1441
Hpy166II GTNNAC 7 cut(s) 189, 405, 869, 1069, 2030, 2068, 2259
Hpy8I GTNNAC 7 cut(s) 189, 405, 869, 1069, 2030, 2068, 2259
Hpy99I CGWCG 5 cut(s) 28, 89, 135, 190, 1917
HpyAV CCTTC 9 cut(s) 159, 273, 399, 456, 1057, 1318, 1333, 1769, 2069
HpyCH4III ACNGT 8 cut(s) 117, 224, 591, 1525, 1563, 1867, 2027, 2226
HpyCH4IV ACGT 3 cut(s) 379, 1274, 1396
HpySE526I ACGT 3 cut(s) 379, 1274, 1396
Hsp92I GRCGYC 1 cut(s) 1912
Hsp92II CATG 7 cut(s) 679, 927, 956, 1015, 1270, 1454, 1538
HspAI GCGC 2 cut(s) 1343, 1586
Kzo9I GATC 3 cut(s) 175, 1381, 2189
LmnI GCTCC 2 cut(s) 1825, 2470
Lsp1109I GCAGC 6 cut(s) 67, 288, 322, 325, 1171, 2317
MabI ACCWGGT 1 cut(s) 2364
MaeI CTAG 6 cut(s) 1943, 1961, 2102, 2159, 2352, 2358
MaeII ACGT 3 cut(s) 379, 1274, 1396
MaeIII GTNAC 3 cut(s) 224, 1469, 1916
MalI GATC 3 cut(s) 177, 1383, 2191
MboI GATC 3 cut(s) 175, 1381, 2189
MflI RGATCY 1 cut(s) 2189
MhlI GDGCHC 6 cut(s) 113, 369, 433, 1402, 1570, 1680
MlsI TGGCCA 1 cut(s) 674
MluCI AATT 9 cut(s) 354, 522, 1256, 1698, 1741, 2139, 2328, 2340, 2508
MluI ACGCGT 1 cut(s) 183
MluNI TGGCCA 1 cut(s) 674
MlyI GAGTC 6 cut(s) 29, 400, 1064, 2116, 2159, 2177
MmeI TCCRAC 3 cut(s) 629, 1483, 1632
Mox20I TGGCCA 1 cut(s) 674
Mph1103I ATGCAT 1 cut(s) 301
MscI TGGCCA 1 cut(s) 674
MseI TTAA 4 cut(s) 357, 1553, 1745, 2138
MslI CAYNNNNRTG 1 cut(s) 1427
Msp20I TGGCCA 1 cut(s) 674
MspA1I CMGCKG 1 cut(s) 58
MspI CCGG 5 cut(s) 41, 107, 169, 375, 427
Mva1269I GAATGC 4 cut(s) 144, 1624, 1949, 2176
MvaI CCWGG 4 cut(s) 421, 1475, 1578, 2366
MvnI CGCG 5 cut(s) 185, 210, 1345, 2044, 2460
NciI CCSGG 6 cut(s) 107, 108, 169, 375, 427, 428
NdeI CATATG 1 cut(s) 814
NdeII GATC 3 cut(s) 175, 1381, 2189
NlaIII CATG 7 cut(s) 679, 927, 956, 1015, 1270, 1454, 1538
NlaIV GGNNCC 8 cut(s) 111, 167, 325, 366, 425, 759, 1109, 1992
NmuCI GTSAC 3 cut(s) 224, 1469, 1916
NruI TCGCGA 1 cut(s) 2044
NsbI TGCGCA 1 cut(s) 1587
NsiI ATGCAT 1 cut(s) 301
NspI RCATGY 2 cut(s) 1015, 1454
NspV TTCGAA 2 cut(s) 1337, 1668
OliI CACNNNNGTG 1 cut(s) 1427
PaeI GCATGC 1 cut(s) 1015
PaeR7I CTCGAG 2 cut(s) 432, 2185
PciI ACATGT 1 cut(s) 1450
PctI GAATGC 4 cut(s) 144, 1624, 1949, 2176
PfeI GAWTC 9 cut(s) 259, 601, 941, 1710, 2129, 2153, 2293, 2348, 2382
PflFI GACNNNGTC 1 cut(s) 230
PfoI TCCNGGA 1 cut(s) 419
Ple19I CGATCG 1 cut(s) 178
PleI GAGTC 6 cut(s) 28, 400, 1064, 2115, 2159, 2177
PmaCI CACGTG 1 cut(s) 1397
PmlI CACGTG 1 cut(s) 1397
PpsI GAGTC 6 cut(s) 28, 400, 1064, 2115, 2159, 2177
Ppu21I YACGTR 1 cut(s) 1397
PpuMI RGGWCCY 1 cut(s) 423
PscI ACATGT 1 cut(s) 1450
PshBI ATTAAT 1 cut(s) 2138
PsiI TTATAA 1 cut(s) 2333
Psp5II RGGWCCY 1 cut(s) 423
Psp6I CCWGG 4 cut(s) 419, 1473, 1576, 2364
PspCI CACGTG 1 cut(s) 1397
PspFI CCCAGC 2 cut(s) 121, 369
PspGI CCWGG 4 cut(s) 419, 1473, 1576, 2364
PspN4I GGNNCC 8 cut(s) 111, 167, 325, 366, 425, 759, 1109, 1992
PspOMI GGGCCC 1 cut(s) 109
PspPI GGNCC 8 cut(s) 109, 110, 165, 171, 202, 324, 423, 974
PspPPI RGGWCCY 1 cut(s) 423
PsuI RGATCY 1 cut(s) 2189
PsyI GACNNNGTC 1 cut(s) 230
PvuI CGATCG 1 cut(s) 178
PvuII CAGCTG 1 cut(s) 58
RruI TCGCGA 1 cut(s) 2044
RsaI GTAC 6 cut(s) 495, 565, 692, 1206, 1273, 2490
RsaNI GTAC 6 cut(s) 494, 564, 691, 1205, 1272, 2489
RseI CAYNNNNRTG 1 cut(s) 1427
SalI GTCGAC 1 cut(s) 187
SaqAI TTAA 4 cut(s) 357, 1553, 1745, 2138
Sau3AI GATC 3 cut(s) 175, 1381, 2189
Sau96I GGNCC 8 cut(s) 109, 110, 165, 171, 202, 324, 423, 974
SchI GAGTC 6 cut(s) 29, 400, 1064, 2116, 2159, 2177
SduI GDGCHC 6 cut(s) 113, 369, 433, 1402, 1570, 1680
SexAI ACCWGGT 1 cut(s) 2364
SfcI CTRYAG 2 cut(s) 1167, 1571
Sfr274I CTCGAG 2 cut(s) 432, 2185
SfuI TTCGAA 2 cut(s) 1337, 1668
SinI GGWCC 2 cut(s) 202, 423
SlaI CTCGAG 2 cut(s) 432, 2185
SmaI CCCGGG 2 cut(s) 108, 428
SmiI ATTTAAAT 1 cut(s) 1746
SmiMI CAYNNNNRTG 1 cut(s) 1427
SmlI CTYRAG 5 cut(s) 432, 533, 1154, 2185, 2465
SmoI CTYRAG 5 cut(s) 432, 533, 1154, 2185, 2465
SphI GCATGC 1 cut(s) 1015
Sse9I AATT 9 cut(s) 354, 522, 1256, 1698, 1741, 2139, 2328, 2340, 2508
SspI AATATT 2 cut(s) 577, 849
SspMI CTAG 6 cut(s) 1943, 1961, 2102, 2159, 2352, 2358
StyI CCWWGG 2 cut(s) 977, 2115
SwaI ATTTAAAT 1 cut(s) 1746
TaaI ACNGT 8 cut(s) 117, 224, 591, 1525, 1563, 1867, 2027, 2226
TaiI ACGT 3 cut(s) 382, 1277, 1399
TaqI TCGA 8 cut(s) 26, 130, 188, 433, 1337, 1668, 2186, 2324
TasI AATT 9 cut(s) 354, 522, 1256, 1698, 1741, 2139, 2328, 2340, 2508
TatI WGTACW 2 cut(s) 690, 1204
TauI GCSGC 4 cut(s) 84, 200, 517, 1724
TfiI GAWTC 9 cut(s) 259, 601, 941, 1710, 2129, 2153, 2293, 2348, 2382
Tru1I TTAA 4 cut(s) 357, 1553, 1745, 2138
Tru9I TTAA 4 cut(s) 357, 1553, 1745, 2138
TscAI CASTG 8 cut(s) 120, 594, 675, 1429, 1568, 1870, 2392, 2430
TseFI GTSAC 3 cut(s) 224, 1469, 1916
TseI GCWGC 6 cut(s) 55, 276, 335, 338, 1159, 2305
Tsp45I GTSAC 3 cut(s) 224, 1469, 1916
TspGWI ACGGA 2 cut(s) 85, 228
TspMI CCCGGG 2 cut(s) 106, 426
TspRI CASTG 8 cut(s) 120, 594, 675, 1429, 1568, 1870, 2392, 2430
Tth111I GACNNNGTC 1 cut(s) 230
VpaK11BI GGWCC 2 cut(s) 202, 423
VspI ATTAAT 1 cut(s) 2138
XagI CCTNNNNNAGG 1 cut(s) 1322
XapI RAATTY 4 cut(s) 1256, 1741, 2340, 2508
XbaI TCTAGA 2 cut(s) 1942, 2101
XceI RCATGY 2 cut(s) 1015, 1454
XhoI CTCGAG 2 cut(s) 432, 2185
XmaI CCCGGG 2 cut(s) 106, 426
XmiI GTMKAC 1 cut(s) 188
XspI CTAG 6 cut(s) 1943, 1961, 2102, 2159, 2352, 2358
Zsp2I ATGCAT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.