Prupe.1G577700_v2.0.a1

Ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001000
Physical Location & Seq
Forward (+)
47020757 .. 47031763
11007 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G577700.1

Sequence Viewer

Length: 2775 bp
ATGCTTGATGGCAACGAAGAGCCCTCGAAGCCCAACAAGGCCGAGAGCCCACCACAGCTCAAGAAATCCCGGACCGTCATCACCACCGCCTCTGACGACGCGGGACCCACAAACCCAATTTTCCCGGGCCCACTCTTTCCCGCCGTCCGACGCATCTCCACCGCCGTTGCCTCCCGCCAGTGGCCGCCGCCCGATCGCCGCGCCTCCAATGCGCCCGCGGACCACCGAGACTTCTCCGACCGCGACTGGGTCTTCCCTTCCTTCGTCGTCCCACAGAACACTTCCAAACGCGGCAAGAAGCTCGCCTCCTCTGATTCACGTACGACTGCGGCGCATGGTAAGCTTGTCGACGCGTCACACCGGATTGCGTCCCAGTCTCCGCCTGCTGCGTCGGTGCCGAAGCCGGAGGCTGAGAGGAAGAAATTGAAGGTGGTGCCAAGTGGAGCTCCGGCCTCTTCTTCGAGTGAGTTGACTCGGCTGAGTTCGAGGTGGCCTGGGGAACTTAAACGTCATTTGTTACTAGTTGTGCTTACTTTCACTTGTATATTATCTATAACTTATGTATTTTATCTGCGGCAGAGAGTTGCACAACTTGAGGAATGGTGTACCGAAAAAGATATCGATTCAAATAGCAGCTTTAGTCTTTTTCTGGCTGAGAAGAATCCCTTTTTTTTCCATTTTGCTAATGCTGAAAGAAGAACTGTTGCTTTGTATGCTGTGGTCATTATACTTACTATGCCATTTGTATTGTACAAACATCTTGATTATCTTTCCCAAATAAGGAATCTCTCAAAAAGGATGAAGAGTAACAAAGAGGAGGTTCCCATAAAGAAGAGGATTGCATATATGGTGGATGTGTGTTTCTCTGTCTATCCATATGCGAAACTGCTTGCGCTTCTGTTTGCAACAATATTTCTTATAGGGTTTGGTGGATTGGCATTGTATGCAGTCAATAAAAATAGCTTTGCTGAAGCTCTTTGGCTCTCATGGACTTTTGTTGCTGATTCTGGAAACCATGCTGACACAGAAGGCCTTGGGCCAAGGATAGTTTCTGTCTCTATAAGTTCAGGAGGCATGCTAATATTTGCAATGATGCTTGGGCTTGTTTCTGATGCTATATCAGAGAAGGTTGATTCACTGCGGAAAGGGAAGAGTGAAGTCATTGAAATGAACCACATCCTAATTCTTGGATGGAGTGACAAACTGGGTTCACTTCTAAAGCAGCTAGCAATAGCAAACAAGAGTGTTGGTGGTGGTGTTGTTGTTATACTTGCTGAAAGAGACAAGGAGGAAATGGAGCTGGATATAGCAAAGCTTGAATTTGACTTCATGGGGACCTCTGTTATATGCAGAAGCGGCAGCCCTCTTATACTGGCTGACCTGAAGAAGGTCTCAGTTTCAAAGGCACGTGCTATCATTGTATTGGCATCTGATGAAAATGCAGATCAGAGTGATGCGCGTGCTTTGAGGGTTGTGCTCAGCCTAACTGGGGTGAAAGAGGGTTTGAGGGGTCATATTGTTGTAGAGATGAGTGACCTTGACAATGAGCCTCTGGTGAAGCTTGTTGGAGGAGAACTCATAGAAACAGTTGTAGCACATGATGTGATTGGAAGGTTGATGATACAGTGTGCTCTGCAGCCTGGTCTTGCACAGATATGGGAGGACATATTGGGGTTTGAGAATGCTGAGTTTTACATCAAAAGGTGGCCTCAATTGGATGGTCTTCGTTTTGAAAATGTGCTTATTTCATTTCCTGATGCTATTCCCTGTGGAATTAAGGTTGCTGCAGATGGTGGGAAGATAATTTTAAATCCAGATGATAATTATGTTCTGAAAGAAGGGGATGAGGTTCTTGTTATAGCAGAAGATGATGATACTTATTCTCCATGCTCCTTTCCCAAGGTATGTGCAGGCTCTTGTCCAAAGACAGTTGAACCTCCCAAATACCCTGAGAAGATATTGTTTTGTGGCTGGCGTCGTGACATAGATGATATGATTATGGTTCTAGAGGCATTCTTGCCTCCAGGTTCAGAACTTTGGTTGTTCAATGAAGTTCCTGTAATAGAAAGAGATAGGAAACTTACAGATGGTGGACTGGATGTTTCTAGATTGGAGAACATAAAGCTTGTCCACAAAGAAGGAAATGCTGTGATCAAACGGCATTTAGAGAGTCTTCCTCTGGAGACTTTTGATTCTATATTAATTCTTGCAGATGAATCACTAGAGGACTCTGTTGTTCATTCTGACTCAAGATCCCTTGCCACTCTTCTCCTTATACGAGATATACAGTCAAAGCGTCTCCCTGACAAAGATACAAAGACAACTTCTTTACGGTTGTCTGCATTTTCACACAGCTCTTGGATCCGTGAAATGCAGCAAGCTTCAGACAAATCAATAATAATTAGTGAAATTCTGGATTCTAGGACTAGAAACTTGGTCTCAGTGTCTAGAATCAGTGATTATGTGCTATCAAACGAACTGGTTAGTATGGCGCTGGCAATGGTGGCTGAAGACAAGCAAATCAATCGTGTCCTTGAGGAGCTATTTTTTGAACAGGGGAATGAGATGTGCATAAAACCAGCAGAACTCTATTTATATGACCAGGAAGAACTCTGCTTTTATGATATAATGCTTAGGGGTCGTCAAAGGCAGGAAATCGTGATTGGATACCGGCTTGCAAACACAGAGCGGGCCATAATTAACCCATCACCGAAGTCAGAACGAAGAAAATGGTCCCATGATGATGTTTTCGTGGTCATCTCCATGAGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Castor_Poll_mid

Protein Analysis

925

Amino Acids

103.01

Weight (kDa)

5.79

Isoelectric Point (pI)

51.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 394, 433
AccBSI CCGCTC 1 cut(s) 2689
AccI GTMKAC 1 cut(s) 348
AccII CGCG 7 cut(s) 101, 201, 218, 243, 291, 353, 1459
AclWI GGATC 3 cut(s) 2247, 2356, 2369
AcoI YGGCCR 1 cut(s) 182
AcsI RAATTY 2 cut(s) 1319, 2409
AcuI CTGAAG 4 cut(s) 990, 1403, 2367, 2529
AcvI CACGTG 1 cut(s) 1409
AcyI GRCGYC 1 cut(s) 1975
AfaI GTAC 3 cut(s) 322, 607, 752
AfiI CCNNNNNNNGG 5 cut(s) 180, 247, 780, 1387, 1489
AflIII ACRYGT 1 cut(s) 351
AgsI TTSAA 9 cut(s) 427, 627, 1166, 1319, 1401, 1733, 1934, 2047, 2552
AhlI ACTAGT 1 cut(s) 520
AjnI CCWGG 4 cut(s) 493, 1639, 2023, 2601
AjuI GAANNNNNNNTTGG 4 cut(s) 1033, 1065, 2646, 2678
Alw21I GWGCWC 3 cut(s) 448, 1479, 1633
Alw26I GTCTC 8 cut(s) 222, 381, 1060, 1275, 1396, 2177, 2303, 2443
AlwI GGATC 3 cut(s) 2247, 2356, 2369
Ama87I CYCGRG 1 cut(s) 124
AoxI GGCC 9 cut(s) 39, 127, 182, 450, 491, 1030, 1037, 1706, 2691
ApaI GGGCCC 1 cut(s) 131
ApeKI GCWGC 7 cut(s) 386, 633, 1222, 1359, 1636, 1784, 2374
ApoI RAATTY 2 cut(s) 1319, 2409
AseI ATTAAT 1 cut(s) 2201
AspLEI GCGC 6 cut(s) 203, 214, 334, 895, 1459, 2494
AspS9I GGNCC 9 cut(s) 72, 104, 127, 128, 220, 1037, 1335, 2691, 2733
AsuC2I CCSGG 3 cut(s) 70, 125, 126
AsuHPI GGTGA 4 cut(s) 73, 1504, 1567, 2700
AsuNHI GCTAGC 1 cut(s) 1225
AvaI CYCGRG 1 cut(s) 124
AvaII GGWCC 5 cut(s) 72, 104, 220, 1335, 2733
BaeGI GKGCMC 1 cut(s) 131
BamHI GGATCC 1 cut(s) 2361
BanI GGYRCC 2 cut(s) 394, 433
BanII GRGCYC 4 cut(s) 24, 50, 131, 448
BbrPI CACGTG 1 cut(s) 1409
BbsI GAAGAC 4 cut(s) 244, 1715, 2165, 2517
Bbv12I GWGCWC 3 cut(s) 448, 1479, 1633
BbvI GCAGC 7 cut(s) 373, 645, 1234, 1371, 1648, 1771, 2386
BccI CCATC 6 cut(s) 2, 1185, 1712, 1784, 2081, 2713
BceAI ACGGC 3 cut(s) 128, 149, 2174
BcgI CGANNNNNNTGC 4 cut(s) 283, 317, 2507, 2541
BciT130I CCWGG 4 cut(s) 495, 1641, 2025, 2603
BciVI GTATCC 1 cut(s) 2660
BclI TGATCA 1 cut(s) 2151
BcnI CCSGG 3 cut(s) 70, 125, 126
BcoDI GTCTC 8 cut(s) 222, 381, 1060, 1275, 1396, 2177, 2303, 2443
BcuI ACTAGT 1 cut(s) 520
BfaI CTAG 8 cut(s) 521, 1226, 2006, 2106, 2222, 2421, 2427, 2448
BfmI CTRYAG 2 cut(s) 1634, 1785
BfoI RGCGCY 1 cut(s) 2495
BfuI GTATCC 1 cut(s) 2660
BlpI GCTNAGC 1 cut(s) 1478
Bme1390I CCNGG 7 cut(s) 70, 125, 126, 495, 1641, 2025, 2603
Bme18I GGWCC 5 cut(s) 72, 104, 220, 1335, 2733
BmeT110I CYCGRG 1 cut(s) 124
BmgT120I GGNCC 9 cut(s) 72, 104, 127, 128, 220, 1037, 1335, 2691, 2733
BmiI GGNNCC 9 cut(s) 105, 106, 129, 396, 435, 822, 1336, 2363, 2735
BmrFI CCNGG 7 cut(s) 70, 125, 126, 495, 1641, 2025, 2603
BmrI ACTGGG 4 cut(s) 256, 367, 1214, 1497
BmsI GCATC 6 cut(s) 162, 1083, 1102, 1436, 1444, 1747
BmtI GCTAGC 1 cut(s) 1229
BmuI ACTGGG 4 cut(s) 256, 367, 1214, 1497
BpiI GAAGAC 4 cut(s) 244, 1715, 2165, 2517
BplI GAGNNNNNCTC 4 cut(s) 1560, 1592, 2161, 2193
BpmI CTGGAG 2 cut(s) 2007, 2201
Bpu10I CCTNAGC 1 cut(s) 2633
Bpu1102I GCTNAGC 1 cut(s) 1478
BpuEI CTTGAG 4 cut(s) 44, 614, 2233, 2555
BpuMI CCSGG 3 cut(s) 70, 125, 126
Bsa29I ATCGAT 1 cut(s) 621
BsaAI YACGTR 2 cut(s) 320, 1409
BsaHI GRCGYC 1 cut(s) 1975
BsaI GGTCTC 2 cut(s) 1396, 2443
BsaJI CCNNGG 6 cut(s) 124, 216, 494, 1033, 1040, 1899
BsaWI WCCGGW 1 cut(s) 360
BsaXI ACNNNNNCTCC 2 cut(s) 1867, 1897
Bsc4I CCNNNNNNNGG 5 cut(s) 180, 247, 780, 1387, 1489
Bse118I RCCGGY 1 cut(s) 2670
Bse1I ACTGG 8 cut(s) 178, 251, 373, 1209, 1377, 1492, 2100, 2484
Bse3DI GCAATG 2 cut(s) 1095, 2505
BseBI CCWGG 4 cut(s) 495, 1641, 2025, 2603
BseCI ATCGAT 1 cut(s) 621
BseDI CCNNGG 6 cut(s) 124, 216, 494, 1033, 1040, 1899
BseGI GGATG 7 cut(s) 804, 859, 1176, 1196, 1723, 1849, 2104
BseLI CCNNNNNNNGG 5 cut(s) 180, 247, 780, 1387, 1489
BseMI GCAATG 2 cut(s) 1095, 2505
BseMII CTCAG 8 cut(s) 402, 470, 645, 1407, 1492, 1677, 1941, 2454
BseNI ACTGG 8 cut(s) 178, 251, 373, 1209, 1377, 1492, 2100, 2484
BseRI GAGGAG 4 cut(s) 298, 830, 1584, 2552
BseSI GKGCMC 1 cut(s) 131
BseXI GCAGC 7 cut(s) 373, 645, 1234, 1371, 1648, 1771, 2386
BsgI GTGCAG 1 cut(s) 1929
Bsh1236I CGCG 7 cut(s) 101, 201, 218, 243, 291, 353, 1459
Bsh1285I CGRYCG 2 cut(s) 196, 241
BshFI GGCC 9 cut(s) 41, 129, 184, 452, 493, 1032, 1039, 1708, 2693
BshNI GGYRCC 2 cut(s) 394, 433
BshVI ATCGAT 1 cut(s) 621
BsiEI CGRYCG 2 cut(s) 196, 241
BsiHKAI GWGCWC 3 cut(s) 448, 1479, 1633
BsiHKCI CYCGRG 1 cut(s) 124
BsiSI CCGG 6 cut(s) 70, 125, 361, 404, 449, 2671
BsiWI CGTACG 1 cut(s) 320
BslFI GGGAC 5 cut(s) 117, 254, 355, 1348, 2719
BslI CCNNNNNNNGG 5 cut(s) 180, 247, 780, 1387, 1489
BsmAI GTCTC 8 cut(s) 222, 381, 1060, 1275, 1396, 2177, 2303, 2443
BsmBI CGTCTC 1 cut(s) 2303
BsmFI GGGAC 5 cut(s) 117, 254, 355, 1348, 2719
BsmI GAATGC 2 cut(s) 1687, 2012
BsnI GGCC 9 cut(s) 41, 129, 184, 452, 493, 1032, 1039, 1708, 2693
Bso31I GGTCTC 2 cut(s) 1396, 2443
BsoBI CYCGRG 1 cut(s) 124
Bsp120I GGGCCC 1 cut(s) 127
Bsp1286I GDGCHC 6 cut(s) 24, 50, 131, 448, 1479, 1633
Bsp1407I TGTACA 1 cut(s) 750
Bsp143I GATC 5 cut(s) 193, 1444, 2151, 2252, 2361
Bsp1720I GCTNAGC 1 cut(s) 1478
BspANI GGCC 9 cut(s) 41, 129, 184, 452, 493, 1032, 1039, 1708, 2693
BspCNI CTCAG 8 cut(s) 403, 471, 646, 1406, 1491, 1678, 1942, 2453
BspDI ATCGAT 1 cut(s) 621
BspFNI CGCG 7 cut(s) 101, 201, 218, 243, 291, 353, 1459
BspLI GGNNCC 9 cut(s) 105, 106, 129, 396, 435, 822, 1336, 2363, 2735
BspMAI CTGCAG 2 cut(s) 1638, 1789
BspOI GCTAGC 1 cut(s) 1229
BspPI GGATC 3 cut(s) 2247, 2356, 2369
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 2 cut(s) 394, 433
BspTNI GGTCTC 2 cut(s) 1396, 2443
BsrBI CCGCTC 1 cut(s) 2689
BsrDI GCAATG 2 cut(s) 1095, 2505
BsrFI RCCGGY 1 cut(s) 2670
BsrGI TGTACA 1 cut(s) 750
BsrI ACTGG 8 cut(s) 178, 251, 373, 1209, 1377, 1492, 2100, 2484
BssAI RCCGGY 1 cut(s) 2670
BssECI CCNNGG 6 cut(s) 124, 216, 494, 1033, 1040, 1899
BssMI GATC 5 cut(s) 193, 1444, 2151, 2252, 2361
BssNI GRCGYC 1 cut(s) 1975
BssT1I CCWWGG 3 cut(s) 1033, 1040, 1899
Bst2UI CCWGG 4 cut(s) 495, 1641, 2025, 2603
Bst4CI ACNGT 7 cut(s) 76, 703, 1588, 1626, 1930, 2289, 2334
Bst6I CTCTTC 6 cut(s) 12, 460, 797, 827, 1145, 2271
BstACI GRCGYC 1 cut(s) 1975
BstAPI GCANNNNNTGC 1 cut(s) 944
BstAUI TGTACA 1 cut(s) 750
BstBAI YACGTR 2 cut(s) 320, 1409
BstDEI CTNAG 9 cut(s) 411, 479, 654, 1393, 1478, 1686, 1950, 2440, 2633
BstDSI CCRYGG 1 cut(s) 216
BstENI CCTNNNNNAGG 1 cut(s) 1385
BstF5I GGATG 7 cut(s) 804, 859, 1176, 1196, 1723, 1849, 2104
BstFNI CGCG 7 cut(s) 101, 201, 218, 243, 291, 353, 1459
BstH2I RGCGCY 1 cut(s) 2495
BstHHI GCGC 6 cut(s) 203, 214, 334, 895, 1459, 2494
BstKTI GATC 5 cut(s) 196, 1447, 2154, 2255, 2364
BstMAI GTCTC 8 cut(s) 222, 381, 1060, 1275, 1396, 2177, 2303, 2443
BstMBI GATC 5 cut(s) 193, 1444, 2151, 2252, 2361
BstMCI CGRYCG 2 cut(s) 196, 241
BstMWI GCNNNNNNNGC 7 cut(s) 28, 209, 340, 713, 944, 1356, 2504
BstNI CCWGG 4 cut(s) 495, 1641, 2025, 2603
BstNSI RCATGY 1 cut(s) 1078
BstSCI CCNGG 7 cut(s) 68, 123, 124, 493, 1639, 2023, 2601
BstSFI CTRYAG 2 cut(s) 1634, 1785
BstSLI GKGCMC 1 cut(s) 131
BstUI CGCG 7 cut(s) 101, 201, 218, 243, 291, 353, 1459
BstV1I GCAGC 7 cut(s) 373, 645, 1234, 1371, 1648, 1771, 2386
BstV2I GAAGAC 4 cut(s) 244, 1715, 2165, 2517
BstX2I RGATCY 2 cut(s) 2252, 2361
BstYI RGATCY 2 cut(s) 2252, 2361
Bsu15I ATCGAT 1 cut(s) 621
BsuI GTATCC 1 cut(s) 2660
BsuRI GGCC 9 cut(s) 41, 129, 184, 452, 493, 1032, 1039, 1708, 2693
BsuTUI ATCGAT 1 cut(s) 621
BtgI CCRYGG 1 cut(s) 216
BtsCI GGATG 7 cut(s) 804, 859, 1176, 1196, 1723, 1849, 2104
BtsI GCAGTG 1 cut(s) 1136
BtsIMutI CAGTG 5 cut(s) 185, 1136, 1631, 2448, 2461
CfoI GCGC 6 cut(s) 203, 214, 334, 895, 1459, 2494
Cfr10I RCCGGY 1 cut(s) 2670
Cfr13I GGNCC 9 cut(s) 72, 104, 127, 128, 220, 1037, 1335, 2691, 2733
Cfr42I CCGCGG 1 cut(s) 219
Cfr9I CCCGGG 1 cut(s) 124
ClaI ATCGAT 1 cut(s) 621
CpoI CGGWCCG 1 cut(s) 72
CseI GACGC 8 cut(s) 107, 159, 342, 357, 359, 378, 1964, 2285
Csp6I GTAC 3 cut(s) 321, 606, 751
CspCI CAANNNNNGTGG 2 cut(s) 148, 183
CspI CGGWCCG 1 cut(s) 72
CviAII CATG 9 cut(s) 335, 987, 1016, 1075, 1330, 1598, 1887, 2738, 2764
CviQI GTAC 3 cut(s) 321, 606, 751
DdeI CTNAG 9 cut(s) 411, 479, 654, 1393, 1478, 1686, 1950, 2440, 2633
DpnI GATC 5 cut(s) 195, 1446, 2153, 2254, 2363
DpnII GATC 5 cut(s) 193, 1444, 2151, 2252, 2361
DraI TTTAAA 1 cut(s) 1809
EaeI YGGCCR 1 cut(s) 182
Eam1104I CTCTTC 6 cut(s) 12, 460, 797, 827, 1145, 2271
EarI CTCTTC 6 cut(s) 12, 460, 797, 827, 1145, 2271
EciI GGCGGA 1 cut(s) 369
Ecl136II GAGCTC 1 cut(s) 446
Eco130I CCWWGG 3 cut(s) 1033, 1040, 1899
Eco147I AGGCCT 1 cut(s) 1032
Eco24I GRGCYC 4 cut(s) 24, 50, 131, 448
Eco31I GGTCTC 2 cut(s) 1396, 2443
Eco32I GATATC 1 cut(s) 619
Eco47I GGWCC 5 cut(s) 72, 104, 220, 1335, 2733
Eco53kI GAGCTC 1 cut(s) 446
Eco57I CTGAAG 4 cut(s) 990, 1403, 2367, 2529
Eco72I CACGTG 1 cut(s) 1409
Eco88I CYCGRG 1 cut(s) 124
EcoICRI GAGCTC 1 cut(s) 446
EcoNI CCTNNNNNAGG 1 cut(s) 1385
EcoO109I RGGNCCY 2 cut(s) 104, 1335
EcoRII CCWGG 4 cut(s) 493, 1639, 2023, 2601
EcoRV GATATC 1 cut(s) 619
EcoT14I CCWWGG 3 cut(s) 1033, 1040, 1899
EcoT38I GRGCYC 4 cut(s) 24, 50, 131, 448
ErhI CCWWGG 3 cut(s) 1033, 1040, 1899
Esp3I CGTCTC 1 cut(s) 2303
FaeI CATG 9 cut(s) 338, 990, 1019, 1078, 1333, 1601, 1890, 2741, 2767
FalI AAGNNNNNCTT 2 cut(s) 650, 682
FaqI GGGAC 5 cut(s) 117, 254, 355, 1348, 2719
FatI CATG 9 cut(s) 334, 986, 1015, 1074, 1329, 1597, 1886, 2737, 2763
FauI CCCGC 5 cut(s) 94, 148, 182, 223, 2682
FauNDI CATATG 1 cut(s) 877
FbaI TGATCA 1 cut(s) 2151
FblI GTMKAC 1 cut(s) 348
FokI GGATG 7 cut(s) 811, 866, 1163, 1203, 1730, 1856, 2111
FriOI GRGCYC 4 cut(s) 24, 50, 131, 448
FspBI CTAG 8 cut(s) 521, 1226, 2006, 2106, 2222, 2421, 2427, 2448
GlaI GCGC 6 cut(s) 202, 213, 333, 894, 1458, 2493
GsuI CTGGAG 2 cut(s) 2007, 2201
HaeII RGCGCY 1 cut(s) 2495
HaeIII GGCC 9 cut(s) 41, 129, 184, 452, 493, 1032, 1039, 1708, 2693
HapII CCGG 6 cut(s) 70, 125, 361, 404, 449, 2671
HgaI GACGC 8 cut(s) 107, 159, 342, 357, 359, 378, 1964, 2285
HhaI GCGC 6 cut(s) 203, 214, 334, 895, 1459, 2494
Hin1I GRCGYC 1 cut(s) 1975
Hin1II CATG 9 cut(s) 338, 990, 1019, 1078, 1333, 1601, 1890, 2741, 2767
Hin6I GCGC 6 cut(s) 201, 212, 332, 893, 1457, 2492
HinP1I GCGC 6 cut(s) 201, 212, 332, 893, 1457, 2492
HincII GTYRAC 2 cut(s) 349, 471
HindII GTYRAC 2 cut(s) 349, 471
HindIII AAGCTT 5 cut(s) 341, 1313, 1559, 2123, 2379
HpaII CCGG 6 cut(s) 70, 125, 361, 404, 449, 2671
HphI GGTGA 4 cut(s) 73, 1504, 1567, 2700
Hpy166II GTNNAC 6 cut(s) 349, 471, 606, 1211, 2093, 2131
Hpy8I GTNNAC 6 cut(s) 349, 471, 606, 1211, 2093, 2131
Hpy99I CGWCG 6 cut(s) 101, 153, 269, 353, 394, 1980
HpyAV CCTTC 9 cut(s) 267, 271, 421, 1022, 1120, 1381, 1605, 1832, 2132
HpyCH4III ACNGT 7 cut(s) 76, 703, 1588, 1626, 1930, 2289, 2334
HpyCH4IV ACGT 3 cut(s) 319, 508, 1408
HpyF10VI GCNNNNNNNGC 7 cut(s) 28, 209, 340, 713, 944, 1356, 2504
HpyF3I CTNAG 9 cut(s) 411, 479, 654, 1393, 1478, 1686, 1950, 2440, 2633
HpySE526I ACGT 3 cut(s) 319, 508, 1408
Hsp92I GRCGYC 1 cut(s) 1975
Hsp92II CATG 9 cut(s) 338, 990, 1019, 1078, 1333, 1601, 1890, 2741, 2767
HspAI GCGC 6 cut(s) 201, 212, 332, 893, 1457, 2492
KflI GGGWCCC 1 cut(s) 104
Ksp22I TGATCA 1 cut(s) 2151
KspI CCGCGG 1 cut(s) 219
Kzo9I GATC 5 cut(s) 193, 1444, 2151, 2252, 2361
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 5 cut(s) 443, 451, 1297, 1895, 2539
Lsp1109I GCAGC 7 cut(s) 373, 645, 1234, 1371, 1648, 1771, 2386
LweI GCATC 6 cut(s) 162, 1083, 1102, 1436, 1444, 1747
MaeI CTAG 8 cut(s) 521, 1226, 2006, 2106, 2222, 2421, 2427, 2448
MaeII ACGT 3 cut(s) 319, 508, 1408
MaeIII GTNAC 6 cut(s) 354, 516, 806, 1196, 1532, 1979
MalI GATC 5 cut(s) 195, 1446, 2153, 2254, 2363
MbiI CCGCTC 1 cut(s) 2689
MboI GATC 5 cut(s) 193, 1444, 2151, 2252, 2361
MfeI CAATTG 1 cut(s) 1712
MflI RGATCY 2 cut(s) 2252, 2361
MhlI GDGCHC 6 cut(s) 24, 50, 131, 448, 1479, 1633
MluI ACGCGT 1 cut(s) 351
MlyI GAGTC 4 cut(s) 466, 2179, 2222, 2240
MmeI TCCRAC 3 cut(s) 172, 261, 1546
MseI TTAA 5 cut(s) 504, 1776, 1808, 2201, 2700
MslI CAYNNNNRTG 4 cut(s) 1166, 1654, 2742, 2762
MspA1I CMGCKG 1 cut(s) 218
MspI CCGG 6 cut(s) 70, 125, 361, 404, 449, 2671
MspR9I CCNGG 7 cut(s) 70, 125, 126, 495, 1641, 2025, 2603
MunI CAATTG 1 cut(s) 1712
Mva1269I GAATGC 2 cut(s) 1687, 2012
MvaI CCWGG 4 cut(s) 495, 1641, 2025, 2603
MvnI CGCG 7 cut(s) 101, 201, 218, 243, 291, 353, 1459
MwoI GCNNNNNNNGC 7 cut(s) 28, 209, 340, 713, 944, 1356, 2504
NciI CCSGG 3 cut(s) 70, 125, 126
NdeI CATATG 1 cut(s) 877
NdeII GATC 5 cut(s) 193, 1444, 2151, 2252, 2361
NheI GCTAGC 1 cut(s) 1225
NlaIII CATG 9 cut(s) 338, 990, 1019, 1078, 1333, 1601, 1890, 2741, 2767
NlaIV GGNNCC 9 cut(s) 105, 106, 129, 396, 435, 822, 1336, 2363, 2735
NmeAIII GCCGAG 2 cut(s) 67, 454
NmuCI GTSAC 4 cut(s) 354, 1196, 1532, 1979
NspI RCATGY 1 cut(s) 1078
PaeI GCATGC 1 cut(s) 1078
PceI AGGCCT 1 cut(s) 1032
PciSI GCTCTTC 1 cut(s) 12
PctI GAATGC 2 cut(s) 1687, 2012
Pfl23II CGTACG 1 cut(s) 320
PflFI GACNNNGTC 1 cut(s) 248
PfoI TCCNGGA 1 cut(s) 68
Ple19I CGATCG 1 cut(s) 196
PleI GAGTC 4 cut(s) 466, 2178, 2222, 2240
PmaCI CACGTG 1 cut(s) 1409
PmlI CACGTG 1 cut(s) 1409
PpsI GAGTC 4 cut(s) 466, 2178, 2222, 2240
Ppu21I YACGTR 2 cut(s) 320, 1409
PpuMI RGGWCCY 2 cut(s) 104, 1335
PshBI ATTAAT 1 cut(s) 2201
Psp124BI GAGCTC 1 cut(s) 448
Psp5II RGGWCCY 2 cut(s) 104, 1335
Psp6I CCWGG 4 cut(s) 493, 1639, 2023, 2601
PspCI CACGTG 1 cut(s) 1409
PspGI CCWGG 4 cut(s) 493, 1639, 2023, 2601
PspLI CGTACG 1 cut(s) 320
PspN4I GGNNCC 9 cut(s) 105, 106, 129, 396, 435, 822, 1336, 2363, 2735
PspOMI GGGCCC 1 cut(s) 127
PspPI GGNCC 9 cut(s) 72, 104, 127, 128, 220, 1037, 1335, 2691, 2733
PspPPI RGGWCCY 2 cut(s) 104, 1335
PstI CTGCAG 2 cut(s) 1638, 1789
PsuI RGATCY 2 cut(s) 2252, 2361
PsyI GACNNNGTC 1 cut(s) 248
PvuI CGATCG 1 cut(s) 196
RsaI GTAC 3 cut(s) 322, 607, 752
RsaNI GTAC 3 cut(s) 321, 606, 751
RseI CAYNNNNRTG 4 cut(s) 1166, 1654, 2742, 2762
Rsr2I CGGWCCG 1 cut(s) 72
RsrII CGGWCCG 1 cut(s) 72
SacI GAGCTC 1 cut(s) 448
SacII CCGCGG 1 cut(s) 219
SalI GTCGAC 1 cut(s) 347
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 5 cut(s) 504, 1776, 1808, 2201, 2700
Sau3AI GATC 5 cut(s) 193, 1444, 2151, 2252, 2361
Sau96I GGNCC 9 cut(s) 72, 104, 127, 128, 220, 1037, 1335, 2691, 2733
SchI GAGTC 4 cut(s) 466, 2179, 2222, 2240
ScrFI CCNGG 7 cut(s) 70, 125, 126, 495, 1641, 2025, 2603
SduI GDGCHC 6 cut(s) 24, 50, 131, 448, 1479, 1633
SfaNI GCATC 6 cut(s) 162, 1083, 1102, 1436, 1444, 1747
SfcI CTRYAG 2 cut(s) 1634, 1785
Sfr303I CCGCGG 1 cut(s) 219
SgrBI CCGCGG 1 cut(s) 219
SinI GGWCC 5 cut(s) 72, 104, 220, 1335, 2733
SmaI CCCGGG 1 cut(s) 126
SmiMI CAYNNNNRTG 4 cut(s) 1166, 1654, 2742, 2762
SmlI CTYRAG 4 cut(s) 59, 593, 2248, 2534
SmoI CTYRAG 4 cut(s) 59, 593, 2248, 2534
SpeI ACTAGT 1 cut(s) 520
SphI GCATGC 1 cut(s) 1078
SseBI AGGCCT 1 cut(s) 1032
SspI AATATT 2 cut(s) 912, 1083
SspMI CTAG 8 cut(s) 521, 1226, 2006, 2106, 2222, 2421, 2427, 2448
SstI GAGCTC 1 cut(s) 448
StuI AGGCCT 1 cut(s) 1032
StyD4I CCNGG 7 cut(s) 68, 123, 124, 493, 1639, 2023, 2601
StyI CCWWGG 3 cut(s) 1033, 1040, 1899
TaaI ACNGT 7 cut(s) 76, 703, 1588, 1626, 1930, 2289, 2334
TaiI ACGT 3 cut(s) 322, 511, 1411
TaqI TCGA 5 cut(s) 26, 348, 461, 485, 621
TatI WGTACW 1 cut(s) 750
TauI GCSGC 7 cut(s) 187, 190, 201, 294, 332, 577, 1359
Tru1I TTAA 5 cut(s) 504, 1776, 1808, 2201, 2700
Tru9I TTAA 5 cut(s) 504, 1776, 1808, 2201, 2700
TscAI CASTG 5 cut(s) 185, 1143, 1631, 2448, 2461
TseFI GTSAC 4 cut(s) 354, 1196, 1532, 1979
TseI GCWGC 7 cut(s) 386, 633, 1222, 1359, 1636, 1784, 2374
Tsp45I GTSAC 4 cut(s) 354, 1196, 1532, 1979
TspDTI ATGAA 8 cut(s) 815, 1184, 1318, 1449, 1737, 2064, 2228, 2229
TspGWI ACGGA 1 cut(s) 2354
TspMI CCCGGG 1 cut(s) 124
TspRI CASTG 5 cut(s) 185, 1143, 1631, 2448, 2461
Tth111I GACNNNGTC 1 cut(s) 248
VpaK11BI GGWCC 5 cut(s) 72, 104, 220, 1335, 2733
VspI ATTAAT 1 cut(s) 2201
XagI CCTNNNNNAGG 1 cut(s) 1385
XapI RAATTY 2 cut(s) 1319, 2409
XbaI TCTAGA 3 cut(s) 2005, 2105, 2447
XceI RCATGY 1 cut(s) 1078
XmaI CCCGGG 1 cut(s) 124
XmiI GTMKAC 1 cut(s) 348
XspI CTAG 8 cut(s) 521, 1226, 2006, 2106, 2222, 2421, 2427, 2448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.