Rorug04G0061000

Ion channel CASTOR-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001000
Physical Location & Seq
Forward (+)
9589533 .. 9592197
2665 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0061000.1

Sequence Viewer

Length: 1101 bp
ATGGCCTCTCCACTGGAAATAGAAATACTCGGGTACCCTAGCATTCGTGTTGATGCTAAAAGAAAAGAAGAAGAAAGCAGCTTTGATCATGCTGTTCAGCTAATGCTCTCGTCTGTGCTGCCCATGTCAATGCAGTTGGCAATTGACTTGGGCCTTTTTGATGTCATCTCCGAAGCGGGTACAGATGCCAAGCTCTCTGCGTTAGACATAGCAGCCAAGATTGGCACCAAGAACCCCGACGCGCCGGTTACATTGGACCGCATCCTCAGGCTACTCACCGCACACTCCGTGCTCAGTTGCTCTGTGGTCACTGGCCAAAGGCTTTATAGCCCCACCGCGGTGTCCAAGCATTTTGTGACTAGTGAAGATGGTGCTTCTTTAAGCCCCGTCATGGCATCAATTCAAGCCAATGTCGTCATGAACAGTTGGTCGCAAGTGAAAGATGCAATTGTTGAAGGAGGAATTCCGTTTAATAGGGTCCATGGCAAACACTTCTTTGAGTATACAGATTCGGACCCCAGGTTTAATCAAGTTTTTAATTCGGGAATGGTCAACCTCACCACTTTGGTTATGAAGAGGATTCTTGATAGCTACCAGGGTTTTGAGCACCTTACCCAGGTTGTTGATGTTGGGGGAGGGCTAGGGGTGGCTCTTGGTCTCATCACTTCCAGATATCCTCATATTAAGGGAATCAACTACGACTTGCCCCATGTTATAAAACATGCTCCCCATTATCCTGGTGTGCAACATGTAGGAGGAGACATGTTTTCAAATGTTCCAAGTGGGGATGCCATTTTTATGAAGAACATACTTCATGACTGGATGGATGAGCAGTGCATAAAGCTATTGAAAAATTGTTACATAGCGATTCCAGATAATGGAAAAGTAATAGTTGTGGAAGCACTAGTTTCGGTTGAGCCAGACACTAGCCCAGCTGAGAAGATCACCTCAGATTTTGATGTGCTTATGATGACACTATCCCCAGGAGGGAAGGAAAGGACCCAACACGAATTTATGGACTTAGCAAATGCTGCTGGATTTAGTGGCATTAAATATGAATGCCTTTCCAGTTATCTTCGTGTTATGGAGTTCATAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Castor_Poll_mid

Protein Analysis

366

Amino Acids

40.07

Weight (kDa)

5.5

Isoelectric Point (pI)

36.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 33 - 121 4.5e-18 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 143 - 348 3.4e-65 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 716
Acc65I GGTACC 1 cut(s) 33
AccB1I GGYRCC 2 cut(s) 33, 224
AccB7I CCANNNNNTGG 1 cut(s) 878
AccI GTMKAC 1 cut(s) 503
AccII CGCG 2 cut(s) 242, 338
AciI CCGC 5 cut(s) 176, 259, 279, 336, 338
AcoI YGGCCR 1 cut(s) 313
AcsI RAATTY 2 cut(s) 462, 1010
AdeI CACNNNGTG 1 cut(s) 289
AfaI GTAC 2 cut(s) 35, 181
AfiI CCNNNNNNNGG 5 cut(s) 337, 391, 616, 878, 987
AflIII ACRYGT 2 cut(s) 748, 762
AgsI TTSAA 4 cut(s) 404, 455, 771, 850
AhlI ACTAGT 2 cut(s) 359, 904
AjnI CCWGG 5 cut(s) 518, 594, 615, 736, 982
AleI CACNNNNGTG 1 cut(s) 338
AluBI AGCT 6 cut(s) 81, 100, 193, 591, 844, 935
AluI AGCT 6 cut(s) 81, 100, 193, 591, 844, 935
Alw21I GWGCWC 2 cut(s) 294, 609
Alw26I GTCTC 2 cut(s) 662, 753
Ama87I CYCGRG 1 cut(s) 29
AoxI GGCC 3 cut(s) 3, 151, 313
ApeKI GCWGC 4 cut(s) 78, 118, 212, 1031
ApoI RAATTY 2 cut(s) 462, 1010
Asp718I GGTACC 1 cut(s) 33
AspLEI GCGC 1 cut(s) 244
AspS9I GGNCC 5 cut(s) 151, 256, 478, 514, 999
AsuHPI GGTGA 3 cut(s) 268, 550, 937
AvaI CYCGRG 1 cut(s) 29
AvaII GGWCC 4 cut(s) 256, 478, 514, 999
AxyI CCTNAGG 1 cut(s) 266
BaeI ACNNNNGTAYC 2 cut(s) 17, 50
BalI TGGCCA 1 cut(s) 315
BanI GGYRCC 2 cut(s) 33, 224
Bbv12I GWGCWC 2 cut(s) 294, 609
BbvI GCAGC 4 cut(s) 90, 105, 224, 1018
BccI CCATC 2 cut(s) 362, 817
BcgI CGANNNNNNTGC 2 cut(s) 891, 925
BciT130I CCWGG 5 cut(s) 520, 596, 617, 738, 984
BclI TGATCA 1 cut(s) 85
BcoDI GTCTC 2 cut(s) 662, 753
BcuI ACTAGT 2 cut(s) 359, 904
BfaI CTAG 5 cut(s) 39, 360, 641, 905, 927
BisI GCNGC 4 cut(s) 79, 119, 213, 1032
BlsI GCNGC 4 cut(s) 80, 120, 214, 1033
Bme1390I CCNGG 5 cut(s) 520, 596, 617, 738, 984
Bme18I GGWCC 4 cut(s) 256, 478, 514, 999
BmeT110I CYCGRG 1 cut(s) 29
BmgT120I GGNCC 5 cut(s) 151, 256, 478, 514, 999
BmiI GGNNCC 5 cut(s) 35, 226, 479, 516, 1001
BmrFI CCNGG 5 cut(s) 520, 596, 617, 738, 984
BmsI GCATC 6 cut(s) 43, 175, 270, 404, 433, 778
BsaI GGTCTC 1 cut(s) 662
BsaJI CCNNGG 6 cut(s) 336, 481, 518, 595, 615, 982
Bsc4I CCNNNNNNNGG 5 cut(s) 337, 391, 616, 878, 987
Bse118I RCCGGY 1 cut(s) 244
Bse1I ACTGG 4 cut(s) 18, 316, 824, 1068
Bse21I CCTNAGG 1 cut(s) 266
BseBI CCWGG 5 cut(s) 520, 596, 617, 738, 984
BseDI CCNNGG 6 cut(s) 336, 481, 518, 595, 615, 982
BseGI GGATG 4 cut(s) 261, 793, 828, 832
BseLI CCNNNNNNNGG 5 cut(s) 337, 391, 616, 878, 987
BseMII CTCAG 4 cut(s) 280, 307, 927, 963
BseNI ACTGG 4 cut(s) 18, 316, 824, 1068
BseRI GAGGAG 1 cut(s) 771
BseXI GCAGC 4 cut(s) 90, 105, 224, 1018
BseYI CCCAGC 1 cut(s) 931
Bsh1236I CGCG 2 cut(s) 242, 338
BshFI GGCC 3 cut(s) 5, 153, 315
BshNI GGYRCC 2 cut(s) 33, 224
BsiHKAI GWGCWC 2 cut(s) 294, 609
BsiHKCI CYCGRG 1 cut(s) 29
BsiSI CCGG 1 cut(s) 245
BslI CCNNNNNNNGG 5 cut(s) 337, 391, 616, 878, 987
BsmAI GTCTC 2 cut(s) 662, 753
BsmI GAATGC 2 cut(s) 42, 1064
BsnI GGCC 3 cut(s) 5, 153, 315
Bso31I GGTCTC 1 cut(s) 662
BsoBI CYCGRG 1 cut(s) 29
Bsp1286I GDGCHC 2 cut(s) 294, 609
Bsp143I GATC 2 cut(s) 85, 942
Bsp19I CCATGG 1 cut(s) 481
BspACI CCGC 5 cut(s) 176, 259, 279, 336, 338
BspANI GGCC 3 cut(s) 5, 153, 315
BspCNI CTCAG 4 cut(s) 279, 306, 928, 962
BspFNI CGCG 2 cut(s) 242, 338
BspHI TCATGA 2 cut(s) 417, 814
BspLI GGNNCC 5 cut(s) 35, 226, 479, 516, 1001
BspT107I GGYRCC 2 cut(s) 33, 224
BspTNI GGTCTC 1 cut(s) 662
BsrFI RCCGGY 1 cut(s) 244
BsrI ACTGG 4 cut(s) 18, 316, 824, 1068
BssAI RCCGGY 1 cut(s) 244
BssECI CCNNGG 6 cut(s) 336, 481, 518, 595, 615, 982
BssMI GATC 2 cut(s) 85, 942
BssNAI GTATAC 1 cut(s) 504
BssT1I CCWWGG 1 cut(s) 481
Bst1107I GTATAC 1 cut(s) 504
Bst2UI CCWGG 5 cut(s) 520, 596, 617, 738, 984
Bst4CI ACNGT 1 cut(s) 425
Bst6I CTCTTC 1 cut(s) 569
BstAPI GCANNNNNTGC 1 cut(s) 1031
BstDEI CTNAG 5 cut(s) 266, 293, 936, 949, 1021
BstDSI CCRYGG 2 cut(s) 336, 481
BstENI CCTNNNNNAGG 1 cut(s) 614
BstF5I GGATG 4 cut(s) 261, 793, 828, 832
BstFNI CGCG 2 cut(s) 242, 338
BstHHI GCGC 1 cut(s) 244
BstKTI GATC 2 cut(s) 88, 945
BstMAI GTCTC 2 cut(s) 662, 753
BstMBI GATC 2 cut(s) 85, 942
BstMWI GCNNNNNNNGC 1 cut(s) 1031
BstNI CCWGG 5 cut(s) 520, 596, 617, 738, 984
BstNSI RCATGY 3 cut(s) 725, 752, 766
BstSCI CCNGG 5 cut(s) 518, 594, 615, 736, 982
BstUI CGCG 2 cut(s) 242, 338
BstV1I GCAGC 4 cut(s) 90, 105, 224, 1018
BstXI CCANNNNNNTGG 1 cut(s) 737
BstZ17I GTATAC 1 cut(s) 504
Bsu36I CCTNAGG 1 cut(s) 266
BsuRI GGCC 3 cut(s) 5, 153, 315
BtgI CCRYGG 2 cut(s) 336, 481
BtsCI GGATG 4 cut(s) 261, 793, 828, 832
BtsI GCAGTG 1 cut(s) 839
BtsIMutI CAGTG 3 cut(s) 11, 309, 839
CciI TCATGA 2 cut(s) 417, 814
CfoI GCGC 1 cut(s) 244
Cfr10I RCCGGY 1 cut(s) 244
Cfr13I GGNCC 5 cut(s) 151, 256, 478, 514, 999
Cfr42I CCGCGG 1 cut(s) 339
CseI GACGC 1 cut(s) 248
Csp6I GTAC 2 cut(s) 34, 180
CviQI GTAC 2 cut(s) 34, 180
DdeI CTNAG 5 cut(s) 266, 293, 936, 949, 1021
DpnI GATC 2 cut(s) 87, 944
DpnII GATC 2 cut(s) 85, 942
DraIII CACNNNGTG 1 cut(s) 289
EaeI YGGCCR 1 cut(s) 313
Eam1104I CTCTTC 1 cut(s) 569
EarI CTCTTC 1 cut(s) 569
Eco130I CCWWGG 1 cut(s) 481
Eco31I GGTCTC 1 cut(s) 662
Eco32I GATATC 1 cut(s) 674
Eco47I GGWCC 4 cut(s) 256, 478, 514, 999
Eco81I CCTNAGG 1 cut(s) 266
Eco88I CYCGRG 1 cut(s) 29
EcoNI CCTNNNNNAGG 1 cut(s) 614
EcoO109I RGGNCCY 1 cut(s) 999
EcoRI GAATTC 1 cut(s) 462
EcoRII CCWGG 5 cut(s) 518, 594, 615, 736, 982
EcoRV GATATC 1 cut(s) 674
EcoT14I CCWWGG 1 cut(s) 481
ErhI CCWWGG 1 cut(s) 481
FauI CCCGC 1 cut(s) 169
FbaI TGATCA 1 cut(s) 85
FblI GTMKAC 1 cut(s) 503
Fnu4HI GCNGC 4 cut(s) 79, 119, 213, 1032
FokI GGATG 4 cut(s) 248, 800, 835, 839
Fsp4HI GCNGC 4 cut(s) 79, 119, 213, 1032
FspBI CTAG 5 cut(s) 39, 360, 641, 905, 927
GlaI GCGC 1 cut(s) 243
GluI GCNGC 4 cut(s) 79, 119, 213, 1032
GsaI CCCAGC 1 cut(s) 935
HaeIII GGCC 3 cut(s) 5, 153, 315
HapII CCGG 1 cut(s) 245
HgaI GACGC 1 cut(s) 248
HhaI GCGC 1 cut(s) 244
Hin6I GCGC 1 cut(s) 242
HinP1I GCGC 1 cut(s) 242
HincII GTYRAC 1 cut(s) 553
HindII GTYRAC 1 cut(s) 553
HinfI GANTC 4 cut(s) 509, 580, 690, 868
HpaII CCGG 1 cut(s) 245
HphI GGTGA 3 cut(s) 268, 550, 937
Hpy166II GTNNAC 2 cut(s) 504, 553
Hpy188I TCNGA 3 cut(s) 172, 514, 952
Hpy188III TCNNGA 6 cut(s) 418, 543, 584, 669, 815, 872
Hpy8I GTNNAC 2 cut(s) 504, 553
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 2 cut(s) 449, 985
HpyCH4III ACNGT 1 cut(s) 425
HpyCH4V TGCA 4 cut(s) 133, 446, 745, 837
HpyF10VI GCNNNNNNNGC 1 cut(s) 1031
HpyF3I CTNAG 5 cut(s) 266, 293, 936, 949, 1021
HspAI GCGC 1 cut(s) 242
KpnI GGTACC 1 cut(s) 37
Ksp22I TGATCA 1 cut(s) 85
KspI CCGCGG 1 cut(s) 339
Kzo9I GATC 2 cut(s) 85, 942
LmnI GCTCC 1 cut(s) 730
Lsp1109I GCAGC 4 cut(s) 90, 105, 224, 1018
LweI GCATC 6 cut(s) 43, 175, 270, 404, 433, 778
MaeI CTAG 5 cut(s) 39, 360, 641, 905, 927
MaeIII GTNAC 4 cut(s) 247, 307, 355, 857
MalI GATC 2 cut(s) 87, 944
MboI GATC 2 cut(s) 85, 942
MboII GAAGA 7 cut(s) 80, 83, 377, 586, 814, 952, 1067
MfeI CAATTG 2 cut(s) 141, 447
MhlI GDGCHC 2 cut(s) 294, 609
MlsI TGGCCA 1 cut(s) 315
MluCI AATT 7 cut(s) 141, 399, 447, 462, 538, 853, 1010
MluNI TGGCCA 1 cut(s) 315
Mox20I TGGCCA 1 cut(s) 315
MscI TGGCCA 1 cut(s) 315
MseI TTAA 6 cut(s) 380, 471, 525, 537, 684, 1050
MslI CAYNNNNRTG 3 cut(s) 128, 338, 797
Msp20I TGGCCA 1 cut(s) 315
MspA1I CMGCKG 2 cut(s) 338, 935
MspI CCGG 1 cut(s) 245
MspR9I CCNGG 5 cut(s) 520, 596, 617, 738, 984
MunI CAATTG 2 cut(s) 141, 447
Mva1269I GAATGC 2 cut(s) 42, 1064
MvaI CCWGG 5 cut(s) 520, 596, 617, 738, 984
MvnI CGCG 2 cut(s) 242, 338
MwoI GCNNNNNNNGC 1 cut(s) 1031
NcoI CCATGG 1 cut(s) 481
NdeII GATC 2 cut(s) 85, 942
NlaIV GGNNCC 5 cut(s) 35, 226, 479, 516, 1001
NmuCI GTSAC 2 cut(s) 307, 355
NspI RCATGY 3 cut(s) 725, 752, 766
OliI CACNNNNGTG 1 cut(s) 338
PagI TCATGA 2 cut(s) 417, 814
PciI ACATGT 2 cut(s) 748, 762
PctI GAATGC 2 cut(s) 42, 1064
PfeI GAWTC 4 cut(s) 509, 580, 690, 868
PflMI CCANNNNNTGG 1 cut(s) 878
PkrI GCNGC 4 cut(s) 80, 120, 214, 1033
PpuMI RGGWCCY 1 cut(s) 999
PscI ACATGT 2 cut(s) 748, 762
PsiI TTATAA 1 cut(s) 716
Psp5II RGGWCCY 1 cut(s) 999
Psp6I CCWGG 5 cut(s) 518, 594, 615, 736, 982
PspFI CCCAGC 1 cut(s) 931
PspGI CCWGG 5 cut(s) 518, 594, 615, 736, 982
PspN4I GGNNCC 5 cut(s) 35, 226, 479, 516, 1001
PspPI GGNCC 5 cut(s) 151, 256, 478, 514, 999
PspPPI RGGWCCY 1 cut(s) 999
PvuII CAGCTG 1 cut(s) 935
RsaI GTAC 2 cut(s) 35, 181
RsaNI GTAC 2 cut(s) 34, 180
RseI CAYNNNNRTG 3 cut(s) 128, 338, 797
SacII CCGCGG 1 cut(s) 339
SaqAI TTAA 6 cut(s) 380, 471, 525, 537, 684, 1050
SatI GCNGC 4 cut(s) 79, 119, 213, 1032
Sau3AI GATC 2 cut(s) 85, 942
Sau96I GGNCC 5 cut(s) 151, 256, 478, 514, 999
ScrFI CCNGG 5 cut(s) 520, 596, 617, 738, 984
SduI GDGCHC 2 cut(s) 294, 609
SfaNI GCATC 6 cut(s) 43, 175, 270, 404, 433, 778
Sfr303I CCGCGG 1 cut(s) 339
SgrBI CCGCGG 1 cut(s) 339
SinI GGWCC 4 cut(s) 256, 478, 514, 999
SmiMI CAYNNNNRTG 3 cut(s) 128, 338, 797
SpeI ACTAGT 2 cut(s) 359, 904
Sse9I AATT 7 cut(s) 141, 399, 447, 462, 538, 853, 1010
SsiI CCGC 5 cut(s) 176, 259, 279, 336, 338
SspMI CTAG 5 cut(s) 39, 360, 641, 905, 927
StyD4I CCNGG 5 cut(s) 518, 594, 615, 736, 982
StyI CCWWGG 1 cut(s) 481
TaaI ACNGT 1 cut(s) 425
TasI AATT 7 cut(s) 141, 399, 447, 462, 538, 853, 1010
TfiI GAWTC 4 cut(s) 509, 580, 690, 868
Tru1I TTAA 6 cut(s) 380, 471, 525, 537, 684, 1050
Tru9I TTAA 6 cut(s) 380, 471, 525, 537, 684, 1050
TscAI CASTG 3 cut(s) 18, 316, 839
TseFI GTSAC 2 cut(s) 307, 355
TseI GCWGC 4 cut(s) 78, 118, 212, 1031
Tsp45I GTSAC 2 cut(s) 307, 355
TspDTI ATGAA 6 cut(s) 434, 587, 803, 815, 1071, 1081
TspGWI ACGGA 2 cut(s) 277, 456
TspRI CASTG 3 cut(s) 18, 316, 839
Van91I CCANNNNNTGG 1 cut(s) 878
VpaK11BI GGWCC 4 cut(s) 256, 478, 514, 999
XagI CCTNNNNNAGG 1 cut(s) 614
XapI RAATTY 2 cut(s) 462, 1010
XceI RCATGY 3 cut(s) 725, 752, 766
XmiI GTMKAC 1 cut(s) 503
XspI CTAG 5 cut(s) 39, 360, 641, 905, 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.