Ion channel
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2333 |
| Acc16I | TGCGCA | 1 cut(s) | 1587 |
| AccB1I | GGYRCC | 1 cut(s) | 364 |
| AccBSI | CCGCTC | 1 cut(s) | 2620 |
| AccI | GTMKAC | 1 cut(s) | 188 |
| AccII | CGCG | 4 cut(s) | 185, 210, 1345, 2044 |
| AclWI | GGATC | 1 cut(s) | 2184 |
| AcoI | YGGCCR | 1 cut(s) | 672 |
| AcsI | RAATTY | 3 cut(s) | 1256, 1741, 2340 |
| AcuI | CTGAAG | 5 cut(s) | 80, 927, 1821, 2298, 2460 |
| AcvI | CACGTG | 1 cut(s) | 1397 |
| AcyI | GRCGYC | 1 cut(s) | 1912 |
| AfaI | GTAC | 6 cut(s) | 495, 565, 692, 1206, 1273, 2655 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 162, 229, 374, 426, 1324, 1426, 1966 |
| AflIII | ACRYGT | 2 cut(s) | 183, 1450 |
| AgsI | TTSAA | 9 cut(s) | 393, 442, 766, 1103, 1256, 1520, 1871, 1984, 2804 |
| AjiI | CACGTC | 1 cut(s) | 380 |
| AjnI | CCWGG | 5 cut(s) | 419, 1473, 1576, 2364, 2532 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 344, 376, 970, 1002, 2577, 2609 |
| AleI | CACNNNNGTG | 1 cut(s) | 1427 |
| Alw21I | GWGCWC | 4 cut(s) | 1402, 1570, 1680, 2544 |
| Alw26I | GTCTC | 6 cut(s) | 997, 1212, 1333, 2114, 2374, 2670 |
| AlwI | GGATC | 1 cut(s) | 2184 |
| Ama87I | CYCGRG | 4 cut(s) | 106, 412, 432, 2185 |
| AoxI | GGCC | 8 cut(s) | 109, 164, 324, 672, 974, 1643, 1849, 2622 |
| ApaI | GGGCCC | 1 cut(s) | 113 |
| ApeKI | GCWGC | 7 cut(s) | 55, 276, 335, 338, 1159, 1721, 2305 |
| ApoI | RAATTY | 3 cut(s) | 1256, 1741, 2340 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 421, 453 |
| AseI | ATTAAT | 1 cut(s) | 2138 |
| AspA2I | CCTAGG | 1 cut(s) | 1960 |
| AspLEI | GCGC | 2 cut(s) | 1345, 1588 |
| AspS9I | GGNCC | 8 cut(s) | 109, 110, 165, 202, 324, 423, 974, 2622 |
| AsuC2I | CCSGG | 4 cut(s) | 107, 108, 375, 427 |
| AsuHPI | GGTGA | 3 cut(s) | 1441, 1504, 2812 |
| AsuII | TTCGAA | 2 cut(s) | 1337, 1668 |
| AvaI | CYCGRG | 4 cut(s) | 106, 412, 432, 2185 |
| AvaII | GGWCC | 2 cut(s) | 202, 423 |
| AvrII | CCTAGG | 1 cut(s) | 1960 |
| BaeGI | GKGCMC | 2 cut(s) | 113, 369 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1263, 1296 |
| BalI | TGGCCA | 1 cut(s) | 674 |
| BanI | GGYRCC | 1 cut(s) | 364 |
| BanII | GRGCYC | 2 cut(s) | 113, 2544 |
| BbrPI | CACGTG | 1 cut(s) | 1397 |
| BbsI | GAAGAC | 3 cut(s) | 1652, 2102, 2228 |
| Bbv12I | GWGCWC | 4 cut(s) | 1402, 1570, 1680, 2544 |
| BbvI | GCAGC | 7 cut(s) | 67, 288, 322, 325, 1171, 1708, 2317 |
| BccI | CCATC | 7 cut(s) | 311, 1649, 1721, 1831, 2018, 2644, 2804 |
| BceAI | ACGGC | 3 cut(s) | 110, 2111, 2609 |
| BcgI | CGANNNNNNTGC | 8 cut(s) | 320, 354, 1358, 1392, 1799, 1833, 2438, 2472 |
| BciT130I | CCWGG | 5 cut(s) | 421, 1475, 1578, 2366, 2534 |
| BcnI | CCSGG | 4 cut(s) | 107, 108, 375, 427 |
| BcoDI | GTCTC | 6 cut(s) | 997, 1212, 1333, 2114, 2374, 2670 |
| BfaI | CTAG | 6 cut(s) | 1943, 1961, 2102, 2159, 2352, 2358 |
| BfmI | CTRYAG | 3 cut(s) | 1167, 1571, 1722 |
| BlnI | CCTAGG | 1 cut(s) | 1960 |
| Bme1390I | CCNGG | 9 cut(s) | 107, 108, 375, 421, 427, 1475, 1578, 2366, 2534 |
| Bme18I | GGWCC | 2 cut(s) | 202, 423 |
| BmeT110I | CYCGRG | 4 cut(s) | 106, 412, 432, 2185 |
| BmgBI | CACGTC | 1 cut(s) | 380 |
| BmgT120I | GGNCC | 8 cut(s) | 109, 110, 165, 202, 324, 423, 974, 2622 |
| BmiI | GGNNCC | 7 cut(s) | 111, 167, 325, 366, 425, 759, 1992 |
| BmrFI | CCNGG | 9 cut(s) | 107, 108, 375, 421, 427, 1475, 1578, 2366, 2534 |
| BmrI | ACTGGG | 3 cut(s) | 238, 1151, 1434 |
| BmuI | ACTGGG | 3 cut(s) | 238, 1151, 1434 |
| BpiI | GAAGAC | 3 cut(s) | 1652, 2102, 2228 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1497, 1529 |
| BpmI | CTGGAG | 1 cut(s) | 1150 |
| Bpu14I | TTCGAA | 2 cut(s) | 1337, 1668 |
| BpuEI | CTTGAG | 4 cut(s) | 554, 1139, 2486, 2812 |
| BpuMI | CCSGG | 4 cut(s) | 107, 108, 375, 427 |
| Bsa29I | ATCGAT | 1 cut(s) | 2324 |
| BsaAI | YACGTR | 1 cut(s) | 1397 |
| BsaHI | GRCGYC | 1 cut(s) | 1912 |
| BsaI | GGTCTC | 3 cut(s) | 1333, 2374, 2670 |
| BsaJI | CCNNGG | 4 cut(s) | 106, 977, 1960, 2115 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1804, 1834 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 162, 229, 374, 426, 1324, 1426, 1966 |
| Bse118I | RCCGGY | 1 cut(s) | 2509 |
| Bse3DI | GCAATG | 1 cut(s) | 2436 |
| BseBI | CCWGG | 5 cut(s) | 421, 1475, 1578, 2366, 2534 |
| BseCI | ATCGAT | 1 cut(s) | 2324 |
| BseDI | CCNNGG | 4 cut(s) | 106, 977, 1960, 2115 |
| BseGI | GGATG | 6 cut(s) | 299, 796, 1364, 1660, 1786, 1983 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 162, 229, 374, 426, 1324, 1426, 1966 |
| BseMI | GCAATG | 1 cut(s) | 2436 |
| BseMII | CTCAG | 7 cut(s) | 326, 333, 1344, 1394, 1614, 1661, 1878 |
| BseRI | GAGGAG | 3 cut(s) | 1521, 2483, 2498 |
| BseSI | GKGCMC | 2 cut(s) | 113, 369 |
| BseXI | GCAGC | 7 cut(s) | 67, 288, 322, 325, 1171, 1708, 2317 |
| BseYI | CCCAGC | 2 cut(s) | 121, 369 |
| Bsh1236I | CGCG | 4 cut(s) | 185, 210, 1345, 2044 |
| Bsh1285I | CGRYCG | 1 cut(s) | 178 |
| BshFI | GGCC | 8 cut(s) | 111, 166, 326, 674, 976, 1645, 1851, 2624 |
| BshNI | GGYRCC | 1 cut(s) | 364 |
| BshVI | ATCGAT | 1 cut(s) | 2324 |
| BsiEI | CGRYCG | 1 cut(s) | 178 |
| BsiHKAI | GWGCWC | 4 cut(s) | 1402, 1570, 1680, 2544 |
| BsiHKCI | CYCGRG | 4 cut(s) | 106, 412, 432, 2185 |
| BsiSI | CCGG | 5 cut(s) | 41, 107, 375, 427, 2510 |
| BslFI | GGGAC | 1 cut(s) | 1461 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 162, 229, 374, 426, 1324, 1426, 1966 |
| BsmAI | GTCTC | 6 cut(s) | 997, 1212, 1333, 2114, 2374, 2670 |
| BsmFI | GGGAC | 1 cut(s) | 1461 |
| BsmI | GAATGC | 3 cut(s) | 1624, 1949, 2176 |
| BsnI | GGCC | 8 cut(s) | 111, 166, 326, 674, 976, 1645, 1851, 2624 |
| Bso31I | GGTCTC | 3 cut(s) | 1333, 2374, 2670 |
| BsoBI | CYCGRG | 4 cut(s) | 106, 412, 432, 2185 |
| Bsp119I | TTCGAA | 2 cut(s) | 1337, 1668 |
| Bsp120I | GGGCCC | 1 cut(s) | 109 |
| Bsp1286I | GDGCHC | 6 cut(s) | 113, 369, 1402, 1570, 1680, 2544 |
| Bsp1407I | TGTACA | 1 cut(s) | 690 |
| Bsp143I | GATC | 4 cut(s) | 7, 175, 1381, 2189 |
| Bsp68I | TCGCGA | 1 cut(s) | 2044 |
| BspANI | GGCC | 8 cut(s) | 111, 166, 326, 674, 976, 1645, 1851, 2624 |
| BspCNI | CTCAG | 7 cut(s) | 325, 334, 1343, 1395, 1615, 1660, 1879 |
| BspDI | ATCGAT | 1 cut(s) | 2324 |
| BspFNI | CGCG | 4 cut(s) | 185, 210, 1345, 2044 |
| BspLI | GGNNCC | 7 cut(s) | 111, 167, 325, 366, 425, 759, 1992 |
| BspMAI | CTGCAG | 1 cut(s) | 1726 |
| BspPI | GGATC | 1 cut(s) | 2184 |
| BspQI | GCTCTTC | 1 cut(s) | 2532 |
| BspT104I | TTCGAA | 2 cut(s) | 1337, 1668 |
| BspT107I | GGYRCC | 1 cut(s) | 364 |
| BspTNI | GGTCTC | 3 cut(s) | 1333, 2374, 2670 |
| BsrBI | CCGCTC | 1 cut(s) | 2620 |
| BsrDI | GCAATG | 1 cut(s) | 2436 |
| BsrFI | RCCGGY | 1 cut(s) | 2509 |
| BsrGI | TGTACA | 1 cut(s) | 690 |
| BssAI | RCCGGY | 1 cut(s) | 2509 |
| BssECI | CCNNGG | 4 cut(s) | 106, 977, 1960, 2115 |
| BssMI | GATC | 4 cut(s) | 7, 175, 1381, 2189 |
| BssNI | GRCGYC | 1 cut(s) | 1912 |
| BssT1I | CCWWGG | 3 cut(s) | 977, 1960, 2115 |
| Bst2UI | CCWGG | 5 cut(s) | 421, 1475, 1578, 2366, 2534 |
| Bst4CI | ACNGT | 8 cut(s) | 117, 224, 498, 591, 1525, 1563, 1867, 2226 |
| Bst6I | CTCTTC | 5 cut(s) | 764, 1082, 2208, 2532, 2763 |
| BstACI | GRCGYC | 1 cut(s) | 1912 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 881 |
| BstAUI | TGTACA | 1 cut(s) | 690 |
| BstBAI | YACGTR | 1 cut(s) | 1397 |
| BstBI | TTCGAA | 2 cut(s) | 1337, 1668 |
| BstC8I | GCNNGC | 7 cut(s) | 1013, 1164, 1347, 1849, 1910, 2310, 2622 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1322 |
| BstF5I | GGATG | 6 cut(s) | 299, 796, 1364, 1660, 1786, 1983 |
| BstFNI | CGCG | 4 cut(s) | 185, 210, 1345, 2044 |
| BstHHI | GCGC | 2 cut(s) | 1345, 1588 |
| BstKTI | GATC | 4 cut(s) | 10, 178, 1384, 2192 |
| BstMAI | GTCTC | 6 cut(s) | 997, 1212, 1333, 2114, 2374, 2670 |
| BstMBI | GATC | 4 cut(s) | 7, 175, 1381, 2189 |
| BstMCI | CGRYCG | 1 cut(s) | 178 |
| BstNI | CCWGG | 5 cut(s) | 421, 1475, 1578, 2366, 2534 |
| BstNSI | RCATGY | 2 cut(s) | 1015, 1454 |
| BstSCI | CCNGG | 9 cut(s) | 105, 106, 373, 419, 425, 1473, 1576, 2364, 2532 |
| BstSFI | CTRYAG | 3 cut(s) | 1167, 1571, 1722 |
| BstSLI | GKGCMC | 2 cut(s) | 113, 369 |
| BstUI | CGCG | 4 cut(s) | 185, 210, 1345, 2044 |
| BstV1I | GCAGC | 7 cut(s) | 67, 288, 322, 325, 1171, 1708, 2317 |
| BstV2I | GAAGAC | 3 cut(s) | 1652, 2102, 2228 |
| BstX2I | RGATCY | 1 cut(s) | 2189 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2701 |
| BstYI | RGATCY | 1 cut(s) | 2189 |
| Bsu15I | ATCGAT | 1 cut(s) | 2324 |
| BsuRI | GGCC | 8 cut(s) | 111, 166, 326, 674, 976, 1645, 1851, 2624 |
| BsuTUI | ATCGAT | 1 cut(s) | 2324 |
| BtgZI | GCGATG | 2 cut(s) | 289, 1040 |
| BtrI | CACGTC | 1 cut(s) | 380 |
| BtsCI | GGATG | 6 cut(s) | 299, 796, 1364, 1660, 1786, 1983 |
| BtsI | GCAGTG | 1 cut(s) | 19 |
| BtuMI | TCGCGA | 1 cut(s) | 2044 |
| Cac8I | GCNNGC | 7 cut(s) | 1013, 1164, 1347, 1849, 1910, 2310, 2622 |
| CfoI | GCGC | 2 cut(s) | 1345, 1588 |
| Cfr10I | RCCGGY | 1 cut(s) | 2509 |
| Cfr13I | GGNCC | 8 cut(s) | 109, 110, 165, 202, 324, 423, 974, 2622 |
| Cfr9I | CCCGGG | 1 cut(s) | 106 |
| ClaI | ATCGAT | 1 cut(s) | 2324 |
| CseI | GACGC | 4 cut(s) | 141, 174, 1901, 2222 |
| CsiI | ACCWGGT | 1 cut(s) | 2364 |
| Csp6I | GTAC | 6 cut(s) | 494, 564, 691, 1205, 1272, 2654 |
| CviAII | CATG | 8 cut(s) | 676, 924, 953, 1012, 1267, 1451, 1535, 2701 |
| CviQI | GTAC | 6 cut(s) | 494, 564, 691, 1205, 1272, 2654 |
| DpnI | GATC | 4 cut(s) | 9, 177, 1383, 2191 |
| DpnII | GATC | 4 cut(s) | 7, 175, 1381, 2189 |
| DraI | TTTAAA | 1 cut(s) | 1746 |
| EaeI | YGGCCR | 1 cut(s) | 672 |
| Eam1104I | CTCTTC | 5 cut(s) | 764, 1082, 2208, 2532, 2763 |
| EarI | CTCTTC | 5 cut(s) | 764, 1082, 2208, 2532, 2763 |
| EciI | GGCGGA | 1 cut(s) | 215 |
| Ecl136II | GAGCTC | 1 cut(s) | 2542 |
| Eco130I | CCWWGG | 3 cut(s) | 977, 1960, 2115 |
| Eco24I | GRGCYC | 2 cut(s) | 113, 2544 |
| Eco31I | GGTCTC | 3 cut(s) | 1333, 2374, 2670 |
| Eco32I | GATATC | 1 cut(s) | 2600 |
| Eco47I | GGWCC | 2 cut(s) | 202, 423 |
| Eco53kI | GAGCTC | 1 cut(s) | 2542 |
| Eco57I | CTGAAG | 5 cut(s) | 80, 927, 1821, 2298, 2460 |
| Eco72I | CACGTG | 1 cut(s) | 1397 |
| Eco88I | CYCGRG | 4 cut(s) | 106, 412, 432, 2185 |
| EcoICRI | GAGCTC | 1 cut(s) | 2542 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1322 |
| EcoO109I | RGGNCCY | 1 cut(s) | 423 |
| EcoRII | CCWGG | 5 cut(s) | 419, 1473, 1576, 2364, 2532 |
| EcoRV | GATATC | 1 cut(s) | 2600 |
| EcoT14I | CCWWGG | 3 cut(s) | 977, 1960, 2115 |
| EcoT22I | ATGCAT | 1 cut(s) | 301 |
| EcoT38I | GRGCYC | 2 cut(s) | 113, 2544 |
| ErhI | CCWWGG | 3 cut(s) | 977, 1960, 2115 |
| FaeI | CATG | 8 cut(s) | 679, 927, 956, 1015, 1270, 1454, 1538, 2704 |
| FaqI | GGGAC | 1 cut(s) | 1461 |
| FatI | CATG | 8 cut(s) | 675, 923, 952, 1011, 1266, 1450, 1534, 2700 |
| FauI | CCCGC | 3 cut(s) | 177, 2574, 2613 |
| FauNDI | CATATG | 1 cut(s) | 814 |
| FblI | GTMKAC | 1 cut(s) | 188 |
| FokI | GGATG | 6 cut(s) | 286, 803, 1351, 1667, 1793, 1990 |
| FriOI | GRGCYC | 2 cut(s) | 113, 2544 |
| FspBI | CTAG | 6 cut(s) | 1943, 1961, 2102, 2159, 2352, 2358 |
| FspI | TGCGCA | 1 cut(s) | 1587 |
| GlaI | GCGC | 2 cut(s) | 1344, 1587 |
| GsaI | CCCAGC | 2 cut(s) | 125, 373 |
| GsuI | CTGGAG | 1 cut(s) | 1150 |
| HaeIII | GGCC | 8 cut(s) | 111, 166, 326, 674, 976, 1645, 1851, 2624 |
| HapII | CCGG | 5 cut(s) | 41, 107, 375, 427, 2510 |
| HgaI | GACGC | 4 cut(s) | 141, 174, 1901, 2222 |
| HhaI | GCGC | 2 cut(s) | 1345, 1588 |
| Hin1I | GRCGYC | 1 cut(s) | 1912 |
| Hin1II | CATG | 8 cut(s) | 679, 927, 956, 1015, 1270, 1454, 1538, 2704 |
| Hin6I | GCGC | 2 cut(s) | 1343, 1586 |
| HinP1I | GCGC | 2 cut(s) | 1343, 1586 |
| HincII | GTYRAC | 4 cut(s) | 189, 405, 1069, 2259 |
| HindII | GTYRAC | 4 cut(s) | 189, 405, 1069, 2259 |
| HindIII | AAGCTT | 2 cut(s) | 1496, 2310 |
| HpaII | CCGG | 5 cut(s) | 41, 107, 375, 427, 2510 |
| HphI | GGTGA | 3 cut(s) | 1441, 1504, 2812 |
| Hpy166II | GTNNAC | 7 cut(s) | 189, 405, 869, 1069, 2030, 2068, 2259 |
| Hpy8I | GTNNAC | 7 cut(s) | 189, 405, 869, 1069, 2030, 2068, 2259 |
| Hpy99I | CGWCG | 5 cut(s) | 28, 89, 135, 190, 1917 |
| HpyAV | CCTTC | 9 cut(s) | 159, 273, 399, 456, 1057, 1318, 1333, 1769, 2069 |
| HpyCH4III | ACNGT | 8 cut(s) | 117, 224, 498, 591, 1525, 1563, 1867, 2226 |
| HpyCH4IV | ACGT | 2 cut(s) | 379, 1396 |
| HpySE526I | ACGT | 2 cut(s) | 379, 1396 |
| Hsp92I | GRCGYC | 1 cut(s) | 1912 |
| Hsp92II | CATG | 8 cut(s) | 679, 927, 956, 1015, 1270, 1454, 1538, 2704 |
| HspAI | GCGC | 2 cut(s) | 1343, 1586 |
| Kzo9I | GATC | 4 cut(s) | 7, 175, 1381, 2189 |
| LguI | GCTCTTC | 1 cut(s) | 2532 |
| LmnI | GCTCC | 2 cut(s) | 1825, 2470 |
| Lsp1109I | GCAGC | 7 cut(s) | 67, 288, 322, 325, 1171, 1708, 2317 |
| MabI | ACCWGGT | 1 cut(s) | 2364 |
| MaeI | CTAG | 6 cut(s) | 1943, 1961, 2102, 2159, 2352, 2358 |
| MaeII | ACGT | 2 cut(s) | 379, 1396 |
| MaeIII | GTNAC | 5 cut(s) | 224, 453, 1469, 1916, 2729 |
| MalI | GATC | 4 cut(s) | 9, 177, 1383, 2191 |
| MbiI | CCGCTC | 1 cut(s) | 2620 |
| MboI | GATC | 4 cut(s) | 7, 175, 1381, 2189 |
| MflI | RGATCY | 1 cut(s) | 2189 |
| MhlI | GDGCHC | 6 cut(s) | 113, 369, 1402, 1570, 1680, 2544 |
| MlsI | TGGCCA | 1 cut(s) | 674 |
| MluI | ACGCGT | 1 cut(s) | 183 |
| MluNI | TGGCCA | 1 cut(s) | 674 |
| MlyI | GAGTC | 6 cut(s) | 400, 424, 1064, 2116, 2159, 2177 |
| MmeI | TCCRAC | 3 cut(s) | 629, 1483, 1632 |
| Mox20I | TGGCCA | 1 cut(s) | 674 |
| Mph1103I | ATGCAT | 1 cut(s) | 301 |
| MscI | TGGCCA | 1 cut(s) | 674 |
| MseI | TTAA | 5 cut(s) | 357, 1553, 1745, 2138, 2796 |
| MslI | CAYNNNNRTG | 3 cut(s) | 1427, 2673, 2699 |
| Msp20I | TGGCCA | 1 cut(s) | 674 |
| MspA1I | CMGCKG | 1 cut(s) | 58 |
| MspI | CCGG | 5 cut(s) | 41, 107, 375, 427, 2510 |
| MspR9I | CCNGG | 9 cut(s) | 107, 108, 375, 421, 427, 1475, 1578, 2366, 2534 |
| Mva1269I | GAATGC | 3 cut(s) | 1624, 1949, 2176 |
| MvaI | CCWGG | 5 cut(s) | 421, 1475, 1578, 2366, 2534 |
| MvnI | CGCG | 4 cut(s) | 185, 210, 1345, 2044 |
| NciI | CCSGG | 4 cut(s) | 107, 108, 375, 427 |
| NdeI | CATATG | 1 cut(s) | 814 |
| NdeII | GATC | 4 cut(s) | 7, 175, 1381, 2189 |
| NlaIII | CATG | 8 cut(s) | 679, 927, 956, 1015, 1270, 1454, 1538, 2704 |
| NlaIV | GGNNCC | 7 cut(s) | 111, 167, 325, 366, 425, 759, 1992 |
| NmuCI | GTSAC | 3 cut(s) | 224, 1469, 1916 |
| NruI | TCGCGA | 1 cut(s) | 2044 |
| NsbI | TGCGCA | 1 cut(s) | 1587 |
| NsiI | ATGCAT | 1 cut(s) | 301 |
| NspI | RCATGY | 2 cut(s) | 1015, 1454 |
| NspV | TTCGAA | 2 cut(s) | 1337, 1668 |
| OliI | CACNNNNGTG | 1 cut(s) | 1427 |
| PaeI | GCATGC | 1 cut(s) | 1015 |
| PaeR7I | CTCGAG | 2 cut(s) | 432, 2185 |
| PciI | ACATGT | 1 cut(s) | 1450 |
| PciSI | GCTCTTC | 1 cut(s) | 2532 |
| PctI | GAATGC | 3 cut(s) | 1624, 1949, 2176 |
| PfeI | GAWTC | 9 cut(s) | 259, 601, 941, 1710, 2129, 2153, 2293, 2348, 2382 |
| PflFI | GACNNNGTC | 1 cut(s) | 230 |
| PfoI | TCCNGGA | 1 cut(s) | 419 |
| Ple19I | CGATCG | 1 cut(s) | 178 |
| PleI | GAGTC | 6 cut(s) | 400, 424, 1064, 2115, 2159, 2177 |
| PmaCI | CACGTG | 1 cut(s) | 1397 |
| PmlI | CACGTG | 1 cut(s) | 1397 |
| PpsI | GAGTC | 6 cut(s) | 400, 424, 1064, 2115, 2159, 2177 |
| Ppu21I | YACGTR | 1 cut(s) | 1397 |
| PpuMI | RGGWCCY | 1 cut(s) | 423 |
| PscI | ACATGT | 1 cut(s) | 1450 |
| PshBI | ATTAAT | 1 cut(s) | 2138 |
| PsiI | TTATAA | 1 cut(s) | 2333 |
| Psp124BI | GAGCTC | 1 cut(s) | 2544 |
| Psp5II | RGGWCCY | 1 cut(s) | 423 |
| Psp6I | CCWGG | 5 cut(s) | 419, 1473, 1576, 2364, 2532 |
| PspCI | CACGTG | 1 cut(s) | 1397 |
| PspFI | CCCAGC | 2 cut(s) | 121, 369 |
| PspGI | CCWGG | 5 cut(s) | 419, 1473, 1576, 2364, 2532 |
| PspN4I | GGNNCC | 7 cut(s) | 111, 167, 325, 366, 425, 759, 1992 |
| PspOMI | GGGCCC | 1 cut(s) | 109 |
| PspPI | GGNCC | 8 cut(s) | 109, 110, 165, 202, 324, 423, 974, 2622 |
| PspPPI | RGGWCCY | 1 cut(s) | 423 |
| PstI | CTGCAG | 1 cut(s) | 1726 |
| PsuI | RGATCY | 1 cut(s) | 2189 |
| PsyI | GACNNNGTC | 1 cut(s) | 230 |
| PvuI | CGATCG | 1 cut(s) | 178 |
| PvuII | CAGCTG | 1 cut(s) | 58 |
| RruI | TCGCGA | 1 cut(s) | 2044 |
| RsaI | GTAC | 6 cut(s) | 495, 565, 692, 1206, 1273, 2655 |
| RsaNI | GTAC | 6 cut(s) | 494, 564, 691, 1205, 1272, 2654 |
| RseI | CAYNNNNRTG | 3 cut(s) | 1427, 2673, 2699 |
| SacI | GAGCTC | 1 cut(s) | 2544 |
| SalI | GTCGAC | 1 cut(s) | 187 |
| SapI | GCTCTTC | 1 cut(s) | 2532 |
| SaqAI | TTAA | 5 cut(s) | 357, 1553, 1745, 2138, 2796 |
| Sau3AI | GATC | 4 cut(s) | 7, 175, 1381, 2189 |
| Sau96I | GGNCC | 8 cut(s) | 109, 110, 165, 202, 324, 423, 974, 2622 |
| SchI | GAGTC | 6 cut(s) | 400, 424, 1064, 2116, 2159, 2177 |
| ScrFI | CCNGG | 9 cut(s) | 107, 108, 375, 421, 427, 1475, 1578, 2366, 2534 |
| SduI | GDGCHC | 6 cut(s) | 113, 369, 1402, 1570, 1680, 2544 |
| SexAI | ACCWGGT | 1 cut(s) | 2364 |
| SfcI | CTRYAG | 3 cut(s) | 1167, 1571, 1722 |
| Sfr274I | CTCGAG | 2 cut(s) | 432, 2185 |
| SfuI | TTCGAA | 2 cut(s) | 1337, 1668 |
| SinI | GGWCC | 2 cut(s) | 202, 423 |
| SlaI | CTCGAG | 2 cut(s) | 432, 2185 |
| SmaI | CCCGGG | 1 cut(s) | 108 |
| SmiI | ATTTAAAT | 1 cut(s) | 1746 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 1427, 2673, 2699 |
| SmlI | CTYRAG | 6 cut(s) | 432, 533, 1154, 2185, 2465, 2827 |
| SmoI | CTYRAG | 6 cut(s) | 432, 533, 1154, 2185, 2465, 2827 |
| SphI | GCATGC | 1 cut(s) | 1015 |
| SspI | AATATT | 3 cut(s) | 577, 849, 2800 |
| SspMI | CTAG | 6 cut(s) | 1943, 1961, 2102, 2159, 2352, 2358 |
| SstI | GAGCTC | 1 cut(s) | 2544 |
| StyD4I | CCNGG | 9 cut(s) | 105, 106, 373, 419, 425, 1473, 1576, 2364, 2532 |
| StyI | CCWWGG | 3 cut(s) | 977, 1960, 2115 |
| SwaI | ATTTAAAT | 1 cut(s) | 1746 |
| TaaI | ACNGT | 8 cut(s) | 117, 224, 498, 591, 1525, 1563, 1867, 2226 |
| TaiI | ACGT | 2 cut(s) | 382, 1399 |
| TaqI | TCGA | 8 cut(s) | 26, 130, 188, 433, 1337, 1668, 2186, 2324 |
| TatI | WGTACW | 2 cut(s) | 690, 1204 |
| TauI | GCSGC | 3 cut(s) | 84, 200, 517 |
| TfiI | GAWTC | 9 cut(s) | 259, 601, 941, 1710, 2129, 2153, 2293, 2348, 2382 |
| Tru1I | TTAA | 5 cut(s) | 357, 1553, 1745, 2138, 2796 |
| Tru9I | TTAA | 5 cut(s) | 357, 1553, 1745, 2138, 2796 |
| TseFI | GTSAC | 3 cut(s) | 224, 1469, 1916 |
| TseI | GCWGC | 7 cut(s) | 55, 276, 335, 338, 1159, 1721, 2305 |
| Tsp45I | GTSAC | 3 cut(s) | 224, 1469, 1916 |
| TspDTI | ATGAA | 9 cut(s) | 466, 912, 1255, 1386, 1674, 1784, 1797, 2166, 2421 |
| TspGWI | ACGGA | 3 cut(s) | 85, 228, 2671 |
| TspMI | CCCGGG | 1 cut(s) | 106 |
| Tth111I | GACNNNGTC | 1 cut(s) | 230 |
| VpaK11BI | GGWCC | 2 cut(s) | 202, 423 |
| VspI | ATTAAT | 1 cut(s) | 2138 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1322 |
| XapI | RAATTY | 3 cut(s) | 1256, 1741, 2340 |
| XbaI | TCTAGA | 2 cut(s) | 1942, 2101 |
| XceI | RCATGY | 2 cut(s) | 1015, 1454 |
| XhoI | CTCGAG | 2 cut(s) | 432, 2185 |
| XmaI | CCCGGG | 1 cut(s) | 106 |
| XmaJI | CCTAGG | 1 cut(s) | 1960 |
| XmiI | GTMKAC | 1 cut(s) | 188 |
| XspI | CTAG | 6 cut(s) | 1943, 1961, 2102, 2159, 2352, 2358 |
| Zsp2I | ATGCAT | 1 cut(s) | 301 |