RchiOBHm_Chr7g0220721

Ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001000
Physical Location & Seq
Reverse (-)
39997090 .. 40012380
15291 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19755

Sequence Viewer

Length: 2691 bp
ATGCTTGACCGCAATGAAGAGTCGTCGAAACCCGACAAGCCGGAAAGCCCACCGCAGCTGAAGAAATCACGGACTATAAGCGGCGACGATGCAACCCCAATCTTCCCGGGCCCACTGTTCCCAGCCGTTCGACGCATCTCCACCATTCCTTCCTCCCACAACTGGCCCCGGCCCGATCGCCACGCGTCGACTACTGCGGCGGACCACCGCGACTTCTCTGACCGTGACTGGGTCTTTCCGTCCTTTGTGCTTCCACAGAATCCTTCCAAACGAAGCAGCAAGAAACACGCCTCCGATGCATCCACCGCTTCTCAGCTTGCGGGGGCCGCCGAAGCTGCTGCTGAGAGGAAGAAAATTAAGTTGGTGCCCCAGCCCGGGCGTGAAGCACCTTCAACGAGCGAGTTGACTCGCCCGAGTTCCAGGACCCGGGCTCGAGATTTGAAACCTTCTTTGTTGCTATTATTTTTGCTCACTTTCATTTGTATATTATCTGTACCTTATGCAGTTTATCTGCGGCAAAGAATTGCAAAACTTGAGGAATGTTGTATCAGTAAAAATGTTGGTACAGATGACAATATTGAGGCTTCACTGTCTGAGAAGAATCCAACAATTTTGCGTTTTCCTAATGCTGAAAGAACTCTTGTTTTGTATTCTGTGGTCTTTACACTGGCCATGCCATTTGTGCTGTACAAACATCTTGATTATTTTTCCCATTTGACAATCCTCTCAAAAAACAAGAATAACAAAGAGGGGGTTTCCTTGAAGAAGAGGATAGCCTATATAGTGGATGTGTGCTTTTCTGTATATCCATATGCTAAACTACTTGCACTTCTCTTTGCAACAATATTACTAATAGGGTTTGGTGGACTGGCATTGTATGCAGTCAATAAAAATAGCTTTGCTGAAGCTCTTTGGCTTTCATGGACTTTTGTTGCTGATTCTGGAAACCATGCTGATACAGAGGGCATTGGGCCAAGGATAGTTTCTGTCTCTATTAGTTCAGGAGGCATGCTTATATTTGCGATGATGCTTGGGCTTGTTTCTGATGCTATATCAGAGAAGGTTGACTCACTAAGGAAAGGGAAGAGTGAAGTCATTGAAAGGAACCACATACTCGTTCTTGGATGGAGTGATAAACTGGGTTCGCTTCTCAAGCAGCTTGCTATAGCAAACAAGAGTGTTGGTGGTGTTGTTGTTGTTGTACTTGCTGAAAGAGACAAGGAGGAAATGGAGATAGATATTGCAAAACTTGAATTTGATTTCATGGGTACGTCTGTTATATGCAGAAGTGGCAGTCCTCTTATACTTGCTGACCTAAAGAAGGTCTCAGTTTCGAAGGCGCGTGCTATCATTGTATTGGCATCTGATGAAAATGCAGATCAGAGTGATGCACGTGCTCTGAGGGTTGTTCTAAGCCTCACTGGGGTGAAAGAGGGTTTGAGGGGACATGTTGTTGTAGAGATGAGTGACCTGGATAATGAGCCTCTTGTGAAGCTTGTTGGAGGAGAACTCATTGAAACAGTAGTAGCACATGATGTGATTGGGCGGTTAATGATACAGTGTGCTCTACAGCCTGGTCTTGCGCAGATATGGGAGGATATATTGGGATTTGAGAATGCTGAGTTTTACATCAAAAGGTGGCCTCAGTTGGATGGTCTTCGTTTCGAAAATGTGCTCATTTCATTTCCTGATGCAATTCCCTGTGGAATCAAGGTTGCCGCAGATGGTGGAAAGATAAATTTAAATCCCGATGATAAATATGTTATGAAAGAAGGGGATGAAGTTCTTGTTATAGCTGAAGATGACGACACTTATGCTCCATCTGCCTTTCCCGAGGTATGTGCTGGCCTTTGTCCAAACACTGTTGAACCTCCCAAATACCCTGAGAAGATATTGTTCTGTGGCTGGCGTCGTGACATAGACGATATGATACTGGTTCTAGAGGCATTCTTGCCTCTAGGTTCAGAACTTTGGATGTTCAATGAGGTTCCAGAAAAAGAAAGAGAGAAGAAACTTACAGATGGTGGACTTGATATTTCGCGATTGGAGAACATAAAACTCGTTCACAAAGAAGGAAATGCTGTGATAAGACGGCATCTAGAGAGTCTTCCCGTGGAGACTTTTGATTCTGTATATGAATCACTAGAGGACTCTGTTGTGCATTCTGACTCGAGATCCCTTGCCACTCTTCTCCTTATACGAGATATACAGTCAAAGCGTCTTCCTTACAAAGAAACAAAGTCAACTTCCTTATCTGCATTTTCGCATAGCTCTTGGATTCGTGAAATGCAGCAAGCTTCAGACAAATCGATAATTATAAGTGAAATTCTGGATTCTAGGACTAGAAACCTGGTCTCTGTTTCCAGAATCAGTGATTATGTGCTGTCAAATGAACTGGTTAGTATGGCACTGGCAATGGTGGCTGAAGATAAGCAAATCAATCGCGTTCTTGAGGAGCTATTTGCGGAGGAGGGGAATGAGATGTGCATAAAACCGGCTGAACTCTATTTATATGACCAGGAAGAGCTCTGCTTTTATGATATAATGCTTAGGGGTCGTCAAAGGCGGGAAGTTGTGATTGGATATCGCCTTGCAAATGCAGAGCGGGCCGTAATCAATCCATCACTGAAATCAGCAGTACGGAAATGGTCTCACGATGATGTTTTTGTGGTCATCTCCATAAGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Castor_Poll_mid

Protein Analysis

896

Amino Acids

99.73

Weight (kDa)

5.63

Isoelectric Point (pI)

46.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Slo-like_RCK PF22614 368 - 472 1.5e-07 Calcium-activated potassium channel slowpoke-like RCK domain
Castor_Poll_mid PF06241 511 - 608 2e-42 Castor and Pollux, part of voltage-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2318
Acc16I TGCGCA 1 cut(s) 1584
AccB1I GGYRCC 1 cut(s) 364
AccBSI CCGCTC 1 cut(s) 2605
AccI GTMKAC 1 cut(s) 188
AccII CGCG 5 cut(s) 185, 210, 1342, 2041, 2445
AclWI GGATC 1 cut(s) 2169
AcoI YGGCCR 1 cut(s) 669
AcsI RAATTY 3 cut(s) 1253, 1738, 2325
AcuI CTGAAG 5 cut(s) 80, 924, 1818, 2283, 2445
AcvI CACGTG 1 cut(s) 1394
AcyI GRCGYC 1 cut(s) 1909
AfaI GTAC 6 cut(s) 495, 565, 689, 1203, 1270, 2640
AfiI CCNNNNNNNGG 7 cut(s) 162, 229, 374, 375, 426, 1321, 1423
AflIII ACRYGT 2 cut(s) 183, 1447
AgsI TTSAA 8 cut(s) 393, 442, 763, 1100, 1253, 1517, 1868, 1981
AjnI CCWGG 5 cut(s) 419, 1470, 1573, 2349, 2517
AjuI GAANNNNNNNTTGG 6 cut(s) 344, 376, 967, 999, 2562, 2594
AleI CACNNNNGTG 1 cut(s) 1424
Alw21I GWGCWC 4 cut(s) 1399, 1567, 1677, 2529
Alw26I GTCTC 6 cut(s) 994, 1209, 1330, 2111, 2359, 2655
AlwI GGATC 1 cut(s) 2169
Ama87I CYCGRG 7 cut(s) 106, 374, 412, 426, 432, 1832, 2170
AoxI GGCC 9 cut(s) 109, 164, 170, 324, 669, 971, 1640, 1846, 2607
ApaI GGGCCC 1 cut(s) 113
ApeKI GCWGC 6 cut(s) 55, 276, 335, 338, 1156, 2290
ApoI RAATTY 3 cut(s) 1253, 1738, 2325
AspLEI GCGC 2 cut(s) 1342, 1585
AspS9I GGNCC 9 cut(s) 109, 110, 165, 171, 202, 324, 423, 971, 2607
AsuC2I CCSGG 7 cut(s) 107, 108, 169, 375, 376, 427, 428
AsuHPI GGTGA 1 cut(s) 1438
AsuII TTCGAA 2 cut(s) 1334, 1665
AvaI CYCGRG 7 cut(s) 106, 374, 412, 426, 432, 1832, 2170
AvaII GGWCC 2 cut(s) 202, 423
BaeGI GKGCMC 2 cut(s) 113, 369
BaeI ACNNNNGTAYC 2 cut(s) 1260, 1293
BalI TGGCCA 1 cut(s) 671
BanI GGYRCC 1 cut(s) 364
BanII GRGCYC 3 cut(s) 113, 433, 2529
BbrPI CACGTG 1 cut(s) 1394
BbsI GAAGAC 3 cut(s) 1649, 2099, 2213
Bbv12I GWGCWC 4 cut(s) 1399, 1567, 1677, 2529
BbvI GCAGC 6 cut(s) 67, 288, 322, 325, 1168, 2302
BccI CCATC 6 cut(s) 1119, 1646, 1718, 1828, 2015, 2629
BceAI ACGGC 3 cut(s) 110, 2108, 2594
BcgI CGANNNNNNTGC 8 cut(s) 320, 354, 1796, 1823, 1830, 1857, 2423, 2457
BciT130I CCWGG 5 cut(s) 421, 1472, 1575, 2351, 2519
BcnI CCSGG 7 cut(s) 107, 108, 169, 375, 376, 427, 428
BcoDI GTCTC 6 cut(s) 994, 1209, 1330, 2111, 2359, 2655
BfaI CTAG 6 cut(s) 1940, 1958, 2099, 2144, 2337, 2343
BfmI CTRYAG 2 cut(s) 1164, 1568
Bme18I GGWCC 2 cut(s) 202, 423
BmeT110I CYCGRG 7 cut(s) 106, 374, 412, 426, 432, 1832, 2170
BmgT120I GGNCC 9 cut(s) 109, 110, 165, 171, 202, 324, 423, 971, 2607
BmiI GGNNCC 7 cut(s) 111, 167, 325, 366, 425, 1106, 1989
BmrI ACTGGG 3 cut(s) 238, 1148, 1431
BmuI ACTGGG 3 cut(s) 238, 1148, 1431
BpiI GAAGAC 3 cut(s) 1649, 2099, 2213
BplI GAGNNNNNCTC 2 cut(s) 1494, 1526
Bpu10I CCTNAGC 1 cut(s) 2549
Bpu14I TTCGAA 2 cut(s) 1334, 1665
BpuEI CTTGAG 3 cut(s) 554, 1136, 2471
BpuMI CCSGG 7 cut(s) 107, 108, 169, 375, 376, 427, 428
Bsa29I ATCGAT 1 cut(s) 2309
BsaAI YACGTR 1 cut(s) 1394
BsaHI GRCGYC 1 cut(s) 1909
BsaI GGTCTC 3 cut(s) 1330, 2359, 2655
BsaJI CCNNGG 7 cut(s) 106, 167, 374, 426, 974, 1833, 2112
BsaXI ACNNNNNCTCC 2 cut(s) 1801, 1831
Bsc4I CCNNNNNNNGG 7 cut(s) 162, 229, 374, 375, 426, 1321, 1423
Bse118I RCCGGY 1 cut(s) 2494
Bse1I ACTGG 9 cut(s) 167, 233, 672, 873, 1143, 1426, 1938, 2400, 2415
Bse3DI GCAATG 2 cut(s) 19, 2421
BseBI CCWGG 5 cut(s) 421, 1472, 1575, 2351, 2519
BseCI ATCGAT 1 cut(s) 2309
BseDI CCNNGG 7 cut(s) 106, 167, 374, 426, 974, 1833, 2112
BseGI GGATG 6 cut(s) 299, 793, 1130, 1657, 1783, 1980
BseLI CCNNNNNNNGG 7 cut(s) 162, 229, 374, 375, 426, 1321, 1423
BseMI GCAATG 2 cut(s) 19, 2421
BseMII CTCAG 8 cut(s) 326, 333, 585, 1341, 1391, 1611, 1658, 1875
BseNI ACTGG 9 cut(s) 167, 233, 672, 873, 1143, 1426, 1938, 2400, 2415
BseRI GAGGAG 3 cut(s) 1518, 2468, 2483
BseSI GKGCMC 2 cut(s) 113, 369
BseXI GCAGC 6 cut(s) 67, 288, 322, 325, 1168, 2302
BseYI CCCAGC 2 cut(s) 121, 369
Bsh1236I CGCG 5 cut(s) 185, 210, 1342, 2041, 2445
Bsh1285I CGRYCG 1 cut(s) 178
BshFI GGCC 9 cut(s) 111, 166, 172, 326, 671, 973, 1642, 1848, 2609
BshNI GGYRCC 1 cut(s) 364
BshVI ATCGAT 1 cut(s) 2309
BsiEI CGRYCG 1 cut(s) 178
BsiHKAI GWGCWC 4 cut(s) 1399, 1567, 1677, 2529
BsiHKCI CYCGRG 7 cut(s) 106, 374, 412, 426, 432, 1832, 2170
BsiSI CCGG 6 cut(s) 41, 107, 169, 375, 427, 2495
BslFI GGGAC 1 cut(s) 1458
BslI CCNNNNNNNGG 7 cut(s) 162, 229, 374, 375, 426, 1321, 1423
BsmAI GTCTC 6 cut(s) 994, 1209, 1330, 2111, 2359, 2655
BsmFI GGGAC 1 cut(s) 1458
BsmI GAATGC 3 cut(s) 1621, 1946, 2161
BsnI GGCC 9 cut(s) 111, 166, 172, 326, 671, 973, 1642, 1848, 2609
Bso31I GGTCTC 3 cut(s) 1330, 2359, 2655
BsoBI CYCGRG 7 cut(s) 106, 374, 412, 426, 432, 1832, 2170
Bsp119I TTCGAA 2 cut(s) 1334, 1665
Bsp120I GGGCCC 1 cut(s) 109
Bsp1286I GDGCHC 7 cut(s) 113, 369, 433, 1399, 1567, 1677, 2529
Bsp1407I TGTACA 1 cut(s) 687
Bsp143I GATC 3 cut(s) 175, 1378, 2174
Bsp68I TCGCGA 1 cut(s) 2041
BspANI GGCC 9 cut(s) 111, 166, 172, 326, 671, 973, 1642, 1848, 2609
BspCNI CTCAG 8 cut(s) 325, 334, 586, 1340, 1392, 1612, 1657, 1876
BspDI ATCGAT 1 cut(s) 2309
BspFNI CGCG 5 cut(s) 185, 210, 1342, 2041, 2445
BspLI GGNNCC 7 cut(s) 111, 167, 325, 366, 425, 1106, 1989
BspPI GGATC 1 cut(s) 2169
BspQI GCTCTTC 1 cut(s) 2517
BspT104I TTCGAA 2 cut(s) 1334, 1665
BspT107I GGYRCC 1 cut(s) 364
BspTNI GGTCTC 3 cut(s) 1330, 2359, 2655
BsrBI CCGCTC 1 cut(s) 2605
BsrDI GCAATG 2 cut(s) 19, 2421
BsrFI RCCGGY 1 cut(s) 2494
BsrGI TGTACA 1 cut(s) 687
BsrI ACTGG 9 cut(s) 167, 233, 672, 873, 1143, 1426, 1938, 2400, 2415
BssAI RCCGGY 1 cut(s) 2494
BssECI CCNNGG 7 cut(s) 106, 167, 374, 426, 974, 1833, 2112
BssMI GATC 3 cut(s) 175, 1378, 2174
BssNI GRCGYC 1 cut(s) 1909
BssT1I CCWWGG 1 cut(s) 974
Bst2UI CCWGG 5 cut(s) 421, 1472, 1575, 2351, 2519
Bst4CI ACNGT 7 cut(s) 117, 224, 591, 1522, 1560, 1864, 2211
Bst6I CTCTTC 5 cut(s) 12, 761, 1079, 2193, 2517
BstACI GRCGYC 1 cut(s) 1909
BstAPI GCANNNNNTGC 1 cut(s) 878
BstAUI TGTACA 1 cut(s) 687
BstBAI YACGTR 1 cut(s) 1394
BstBI TTCGAA 2 cut(s) 1334, 1665
BstC8I GCNNGC 8 cut(s) 318, 1010, 1161, 1344, 1846, 1907, 2295, 2607
BstDSI CCRYGG 1 cut(s) 2112
BstENI CCTNNNNNAGG 1 cut(s) 1319
BstF5I GGATG 6 cut(s) 299, 793, 1130, 1657, 1783, 1980
BstFNI CGCG 5 cut(s) 185, 210, 1342, 2041, 2445
BstHHI GCGC 2 cut(s) 1342, 1585
BstKTI GATC 3 cut(s) 178, 1381, 2177
BstMAI GTCTC 6 cut(s) 994, 1209, 1330, 2111, 2359, 2655
BstMBI GATC 3 cut(s) 175, 1378, 2174
BstMCI CGRYCG 1 cut(s) 178
BstNI CCWGG 5 cut(s) 421, 1472, 1575, 2351, 2519
BstNSI RCATGY 2 cut(s) 1012, 1451
BstSFI CTRYAG 2 cut(s) 1164, 1568
BstSLI GKGCMC 2 cut(s) 113, 369
BstUI CGCG 5 cut(s) 185, 210, 1342, 2041, 2445
BstV1I GCAGC 6 cut(s) 67, 288, 322, 325, 1168, 2302
BstV2I GAAGAC 3 cut(s) 1649, 2099, 2213
BstX2I RGATCY 1 cut(s) 2174
BstYI RGATCY 1 cut(s) 2174
Bsu15I ATCGAT 1 cut(s) 2309
BsuRI GGCC 9 cut(s) 111, 166, 172, 326, 671, 973, 1642, 1848, 2609
BsuTUI ATCGAT 1 cut(s) 2309
BtgI CCRYGG 1 cut(s) 2112
BtgZI GCGATG 1 cut(s) 1037
BtsCI GGATG 6 cut(s) 299, 793, 1130, 1657, 1783, 1980
BtsIMutI CAGTG 9 cut(s) 113, 587, 665, 1419, 1565, 1860, 2377, 2408, 2624
BtuMI TCGCGA 1 cut(s) 2041
Cac8I GCNNGC 8 cut(s) 318, 1010, 1161, 1344, 1846, 1907, 2295, 2607
CfoI GCGC 2 cut(s) 1342, 1585
Cfr10I RCCGGY 1 cut(s) 2494
Cfr13I GGNCC 9 cut(s) 109, 110, 165, 171, 202, 324, 423, 971, 2607
Cfr9I CCCGGG 3 cut(s) 106, 374, 426
ClaI ATCGAT 1 cut(s) 2309
CseI GACGC 4 cut(s) 141, 174, 1898, 2207
CsiI ACCWGGT 1 cut(s) 2349
Csp6I GTAC 6 cut(s) 494, 564, 688, 1202, 1269, 2639
CviAII CATG 7 cut(s) 673, 921, 950, 1009, 1264, 1448, 1532
CviQI GTAC 6 cut(s) 494, 564, 688, 1202, 1269, 2639
DpnI GATC 3 cut(s) 177, 1380, 2176
DpnII GATC 3 cut(s) 175, 1378, 2174
DraI TTTAAA 1 cut(s) 1743
EaeI YGGCCR 1 cut(s) 669
Eam1104I CTCTTC 5 cut(s) 12, 761, 1079, 2193, 2517
EarI CTCTTC 5 cut(s) 12, 761, 1079, 2193, 2517
EciI GGCGGA 1 cut(s) 215
Ecl136II GAGCTC 1 cut(s) 2527
Eco130I CCWWGG 1 cut(s) 974
Eco24I GRGCYC 3 cut(s) 113, 433, 2529
Eco31I GGTCTC 3 cut(s) 1330, 2359, 2655
Eco32I GATATC 1 cut(s) 2585
Eco47I GGWCC 2 cut(s) 202, 423
Eco53kI GAGCTC 1 cut(s) 2527
Eco57I CTGAAG 5 cut(s) 80, 924, 1818, 2283, 2445
Eco72I CACGTG 1 cut(s) 1394
Eco88I CYCGRG 7 cut(s) 106, 374, 412, 426, 432, 1832, 2170
EcoICRI GAGCTC 1 cut(s) 2527
EcoNI CCTNNNNNAGG 1 cut(s) 1319
EcoO109I RGGNCCY 1 cut(s) 423
EcoRII CCWGG 5 cut(s) 419, 1470, 1573, 2349, 2517
EcoRV GATATC 1 cut(s) 2585
EcoT14I CCWWGG 1 cut(s) 974
EcoT22I ATGCAT 1 cut(s) 301
EcoT38I GRGCYC 3 cut(s) 113, 433, 2529
ErhI CCWWGG 1 cut(s) 974
FaeI CATG 7 cut(s) 676, 924, 953, 1012, 1267, 1451, 1535
FaqI GGGAC 1 cut(s) 1458
FatI CATG 7 cut(s) 672, 920, 949, 1008, 1263, 1447, 1531
FauI CCCGC 3 cut(s) 313, 2559, 2598
FauNDI CATATG 1 cut(s) 811
FblI GTMKAC 1 cut(s) 188
FokI GGATG 6 cut(s) 286, 800, 1137, 1664, 1790, 1987
FriOI GRGCYC 3 cut(s) 113, 433, 2529
FspBI CTAG 6 cut(s) 1940, 1958, 2099, 2144, 2337, 2343
FspI TGCGCA 1 cut(s) 1584
GlaI GCGC 2 cut(s) 1341, 1584
GsaI CCCAGC 2 cut(s) 125, 373
HaeIII GGCC 9 cut(s) 111, 166, 172, 326, 671, 973, 1642, 1848, 2609
HapII CCGG 6 cut(s) 41, 107, 169, 375, 427, 2495
HgaI GACGC 4 cut(s) 141, 174, 1898, 2207
HhaI GCGC 2 cut(s) 1342, 1585
Hin1I GRCGYC 1 cut(s) 1909
Hin1II CATG 7 cut(s) 676, 924, 953, 1012, 1267, 1451, 1535
Hin6I GCGC 2 cut(s) 1340, 1583
HinP1I GCGC 2 cut(s) 1340, 1583
HincII GTYRAC 4 cut(s) 189, 405, 1066, 2244
HindII GTYRAC 4 cut(s) 189, 405, 1066, 2244
HindIII AAGCTT 2 cut(s) 1493, 2295
HpaII CCGG 6 cut(s) 41, 107, 169, 375, 427, 2495
HphI GGTGA 1 cut(s) 1438
Hpy166II GTNNAC 7 cut(s) 189, 405, 866, 1066, 2027, 2065, 2244
Hpy8I GTNNAC 7 cut(s) 189, 405, 866, 1066, 2027, 2065, 2244
Hpy99I CGWCG 5 cut(s) 28, 89, 135, 190, 1914
HpyAV CCTTC 9 cut(s) 159, 273, 399, 456, 1054, 1315, 1330, 1766, 2066
HpyCH4III ACNGT 7 cut(s) 117, 224, 591, 1522, 1560, 1864, 2211
HpyCH4IV ACGT 2 cut(s) 1271, 1393
HpySE526I ACGT 2 cut(s) 1271, 1393
Hsp92I GRCGYC 1 cut(s) 1909
Hsp92II CATG 7 cut(s) 676, 924, 953, 1012, 1267, 1451, 1535
HspAI GCGC 2 cut(s) 1340, 1583
Kzo9I GATC 3 cut(s) 175, 1378, 2174
LguI GCTCTTC 1 cut(s) 2517
LmnI GCTCC 2 cut(s) 1822, 2455
Lsp1109I GCAGC 6 cut(s) 67, 288, 322, 325, 1168, 2302
MabI ACCWGGT 1 cut(s) 2349
MaeI CTAG 6 cut(s) 1940, 1958, 2099, 2144, 2337, 2343
MaeII ACGT 2 cut(s) 1271, 1393
MaeIII GTNAC 3 cut(s) 224, 1466, 1913
MalI GATC 3 cut(s) 177, 1380, 2176
MbiI CCGCTC 1 cut(s) 2605
MboI GATC 3 cut(s) 175, 1378, 2174
MflI RGATCY 1 cut(s) 2174
MhlI GDGCHC 7 cut(s) 113, 369, 433, 1399, 1567, 1677, 2529
MlsI TGGCCA 1 cut(s) 671
MluCI AATT 8 cut(s) 354, 522, 609, 1253, 1695, 1738, 2313, 2325
MluI ACGCGT 1 cut(s) 183
MluNI TGGCCA 1 cut(s) 671
MlyI GAGTC 6 cut(s) 29, 400, 1061, 2113, 2144, 2162
MmeI TCCRAC 3 cut(s) 629, 1480, 1629
Mox20I TGGCCA 1 cut(s) 671
Mph1103I ATGCAT 1 cut(s) 301
MscI TGGCCA 1 cut(s) 671
MseI TTAA 3 cut(s) 357, 1550, 1742
MslI CAYNNNNRTG 2 cut(s) 1424, 2658
Msp20I TGGCCA 1 cut(s) 671
MspA1I CMGCKG 1 cut(s) 58
MspI CCGG 6 cut(s) 41, 107, 169, 375, 427, 2495
Mva1269I GAATGC 3 cut(s) 1621, 1946, 2161
MvaI CCWGG 5 cut(s) 421, 1472, 1575, 2351, 2519
MvnI CGCG 5 cut(s) 185, 210, 1342, 2041, 2445
NciI CCSGG 7 cut(s) 107, 108, 169, 375, 376, 427, 428
NdeI CATATG 1 cut(s) 811
NdeII GATC 3 cut(s) 175, 1378, 2174
NlaIII CATG 7 cut(s) 676, 924, 953, 1012, 1267, 1451, 1535
NlaIV GGNNCC 7 cut(s) 111, 167, 325, 366, 425, 1106, 1989
NmuCI GTSAC 3 cut(s) 224, 1466, 1913
NruI TCGCGA 1 cut(s) 2041
NsbI TGCGCA 1 cut(s) 1584
NsiI ATGCAT 1 cut(s) 301
NspI RCATGY 2 cut(s) 1012, 1451
NspV TTCGAA 2 cut(s) 1334, 1665
OliI CACNNNNGTG 1 cut(s) 1424
PaeI GCATGC 1 cut(s) 1012
PaeR7I CTCGAG 2 cut(s) 432, 2170
PciI ACATGT 1 cut(s) 1447
PciSI GCTCTTC 1 cut(s) 2517
PctI GAATGC 3 cut(s) 1621, 1946, 2161
PfeI GAWTC 9 cut(s) 259, 601, 938, 1707, 2126, 2138, 2278, 2333, 2367
PflFI GACNNNGTC 1 cut(s) 230
PfoI TCCNGGA 1 cut(s) 419
Ple19I CGATCG 1 cut(s) 178
PleI GAGTC 6 cut(s) 28, 400, 1061, 2112, 2144, 2162
PmaCI CACGTG 1 cut(s) 1394
PmlI CACGTG 1 cut(s) 1394
PpsI GAGTC 6 cut(s) 28, 400, 1061, 2112, 2144, 2162
Ppu21I YACGTR 1 cut(s) 1394
PpuMI RGGWCCY 1 cut(s) 423
PscI ACATGT 1 cut(s) 1447
PsiI TTATAA 1 cut(s) 2318
Psp124BI GAGCTC 1 cut(s) 2529
Psp5II RGGWCCY 1 cut(s) 423
Psp6I CCWGG 5 cut(s) 419, 1470, 1573, 2349, 2517
PspCI CACGTG 1 cut(s) 1394
PspFI CCCAGC 2 cut(s) 121, 369
PspGI CCWGG 5 cut(s) 419, 1470, 1573, 2349, 2517
PspN4I GGNNCC 7 cut(s) 111, 167, 325, 366, 425, 1106, 1989
PspOMI GGGCCC 1 cut(s) 109
PspPI GGNCC 9 cut(s) 109, 110, 165, 171, 202, 324, 423, 971, 2607
PspPPI RGGWCCY 1 cut(s) 423
PsuI RGATCY 1 cut(s) 2174
PsyI GACNNNGTC 1 cut(s) 230
PvuI CGATCG 1 cut(s) 178
PvuII CAGCTG 1 cut(s) 58
RruI TCGCGA 1 cut(s) 2041
RsaI GTAC 6 cut(s) 495, 565, 689, 1203, 1270, 2640
RsaNI GTAC 6 cut(s) 494, 564, 688, 1202, 1269, 2639
RseI CAYNNNNRTG 2 cut(s) 1424, 2658
SacI GAGCTC 1 cut(s) 2529
SalI GTCGAC 1 cut(s) 187
SapI GCTCTTC 1 cut(s) 2517
SaqAI TTAA 3 cut(s) 357, 1550, 1742
Sau3AI GATC 3 cut(s) 175, 1378, 2174
Sau96I GGNCC 9 cut(s) 109, 110, 165, 171, 202, 324, 423, 971, 2607
SchI GAGTC 6 cut(s) 29, 400, 1061, 2113, 2144, 2162
SduI GDGCHC 7 cut(s) 113, 369, 433, 1399, 1567, 1677, 2529
SexAI ACCWGGT 1 cut(s) 2349
SfcI CTRYAG 2 cut(s) 1164, 1568
Sfr274I CTCGAG 2 cut(s) 432, 2170
SfuI TTCGAA 2 cut(s) 1334, 1665
SinI GGWCC 2 cut(s) 202, 423
SlaI CTCGAG 2 cut(s) 432, 2170
SmaI CCCGGG 3 cut(s) 108, 376, 428
SmiI ATTTAAAT 1 cut(s) 1743
SmiMI CAYNNNNRTG 2 cut(s) 1424, 2658
SmlI CTYRAG 5 cut(s) 432, 533, 1151, 2170, 2450
SmoI CTYRAG 5 cut(s) 432, 533, 1151, 2170, 2450
SphI GCATGC 1 cut(s) 1012
SrfI GCCCGGGC 1 cut(s) 376
Sse9I AATT 8 cut(s) 354, 522, 609, 1253, 1695, 1738, 2313, 2325
SspI AATATT 2 cut(s) 577, 846
SspMI CTAG 6 cut(s) 1940, 1958, 2099, 2144, 2337, 2343
SstI GAGCTC 1 cut(s) 2529
StyI CCWWGG 1 cut(s) 974
SwaI ATTTAAAT 1 cut(s) 1743
TaaI ACNGT 7 cut(s) 117, 224, 591, 1522, 1560, 1864, 2211
TaiI ACGT 2 cut(s) 1274, 1396
TaqI TCGA 8 cut(s) 26, 130, 188, 433, 1334, 1665, 2171, 2309
TasI AATT 8 cut(s) 354, 522, 609, 1253, 1695, 1738, 2313, 2325
TatI WGTACW 2 cut(s) 687, 1201
TauI GCSGC 5 cut(s) 84, 200, 329, 517, 1721
TfiI GAWTC 9 cut(s) 259, 601, 938, 1707, 2126, 2138, 2278, 2333, 2367
Tru1I TTAA 3 cut(s) 357, 1550, 1742
Tru9I TTAA 3 cut(s) 357, 1550, 1742
TscAI CASTG 9 cut(s) 120, 594, 672, 1426, 1565, 1867, 2377, 2415, 2631
TseFI GTSAC 3 cut(s) 224, 1466, 1913
TseI GCWGC 6 cut(s) 55, 276, 335, 338, 1156, 2290
Tsp45I GTSAC 3 cut(s) 224, 1466, 1913
TspGWI ACGGA 3 cut(s) 85, 228, 2656
TspMI CCCGGG 3 cut(s) 106, 374, 426
TspRI CASTG 9 cut(s) 120, 594, 672, 1426, 1565, 1867, 2377, 2415, 2631
Tth111I GACNNNGTC 1 cut(s) 230
VpaK11BI GGWCC 2 cut(s) 202, 423
XagI CCTNNNNNAGG 1 cut(s) 1319
XapI RAATTY 3 cut(s) 1253, 1738, 2325
XbaI TCTAGA 2 cut(s) 1939, 2098
XceI RCATGY 2 cut(s) 1012, 1451
XhoI CTCGAG 2 cut(s) 432, 2170
XmaI CCCGGG 3 cut(s) 106, 374, 426
XmiI GTMKAC 1 cut(s) 188
XspI CTAG 6 cut(s) 1940, 1958, 2099, 2144, 2337, 2343
Zsp2I ATGCAT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.