Rmu_sc0001286.1_g000017

Ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001286.1
OG0001000
Physical Location & Seq
Reverse (-)
75057 .. 85743
10687 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001286.1_g000017.1.cds

Sequence Viewer

Length: 2820 bp
atgcttgatcgcaatgaagagtcgtcgaagcccgacaagccggaaagcccaccgcagctgaagaaatcacggactataagcggcgacgatgcaaccccaatcttcccgggcccactgttcccagccgttcgacgcatctccaccattccttcctcccgcaactggcccccgcccgatcgccacgcgccgactagtgcggcggaccaccgcgacttctctgaccgtgactgggtctttccgtcgtttgtgcttccacagaatccttccaaacgaagcagcaagaaacaggcctccgatgcatccaccgcttctcagcttgcgggggccaccgaagctgctgctgagaggaagaaaattaagttggtgccccaagcaccttcaacgagcgagttggctcgcccgagttccaggaccggggctcgagatttgaaaccttatttgttgctattatctttgctcactttcatttgtatattatctgtaccttatgcagtttatctgcggcaaagaattgtaaaacttgaggaatgttgtatcagtaaaaatgttggtacagatgacaatattgaggcttcactgtctgagaagaatccaacaattttgcgttttcctaatgccgaaagaagaactcttgttttgtattctgtggtctttacactggccatgccatttgtgctgtacaaacatcttgattatttttcccatttgacaatcctctcaaaaaacaagaataacaaagagggggttcccttgaagaagaggatagcctatatagtggatgtgtgcttttctgtatatccatatgctaaactacttgcacttctctttgcaacaatattactaatagggtttggtggactggcattgtatgcagtcaataaaaatagctttgctgaagctctttggctttcatggacttttgttgctgattctggaaaccatgctgatacagagggcattgggccaaggatagtttctgtctctattagttcaggaggcatgcttatatttgcgatgatgcttgggcttgtttctgatgctatatcagagaaggttgactcactaaggaaagggaagagtgaagtcattgaaaggaaccacatactcgttcttggatggagtgataaactgggttcgcttctcaagcagcttgctatagcaaacaagagtgttggtggtggtgttgttgttgtacttgctgaaagagacaaggaggaaatggagatagatattgcaaaacttgaatttgatttcatgggtacgtctgttatatgcagaagtggcagtcctcttatacttgctgacctaaagaaggtctcagtttcgaaggcgcgtgctatcattgtattggcatctgatgaaaatgcagatcagagtgatgcacgtgctctgagggttgttctaagcctcactggggtgaaagagggtttgaggggacatgttgttgtagagatgagtgacctggataatgagcctcttgtgaagcttgttggaggagaactcattgaaacagtagtagcacatgatgtgattgggcggttaatgatacagtgtgctctacagcctggtcttgcgcagatatgggaggatatattgggatttgagaatgctgagttttacatcaaaaggtggcctcagttggatggtcttcgtttcgaaaatgtgctcatttcatttcctgatgcaattccctgtggaatcaaggttgccgcagatggtggaaagataaatttaaatcccgatgataaatatgttatgaaagaaggggatgaagttcttgttatagctgaagatgacgacacttatgctccatctgcctttccagagcaggtatgtgctggcctttgtccaaacactgttgaacctcccaaataccctgagaagatattgttctgtggctggcgtcgtgacattgacgatatgatactggttctagaggcattcttgcctctaggttcagaactttggatgttcaatgaggttccagaaaaagaaagagagaagaaacttacagatggtggacttgatatttcgcgattggagaacataaaacttgttcacaaagaaggaaatgctgtgataagacggcatctagagagtcttcccttggagacttttgattctatattaattcttgcagatgaatcactagaggactctgttgtgcattctgactcgagatcccttgccactcttctccttatacgagatatacagtcaaagcgtcttccttacaaagaaacaaagtcaacttcctcatctgcattttcgcatagctcttggattcgtgaaatgcagcaagcttcagataaatcgataattataagtgaaattctggattctaggactagaaacctggtctctgtttccagaatcagtgattatgtgctgtcaaatgaactggttagtatggcactggcaatggtggctgaagataagcaaatcaatcgcgttcttgaggagctatttgcggaggaggggaatgagatgtgcataaaaccggctgaactctatttatatgaccaggaagagctctgcttttatgatataatgcttaggggtcgtcaaaggcgggaagttgtgattggatatcgccttgcaaatgcagagcgggccgtaatcaatccatcactgaaatcagcagtacggaaatggtctcacgatgatgtttttgtgtcattggcttggcttactatgctggaagtaattggttactatactggaagcaaatggtttgatttcagggaggaagagaattggaattgtgtagtgcctgttcatattgaagatggtgtcctcaccaactcaagttcttggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Castor_Poll_mid

Protein Analysis

939

Amino Acids

104.67

Weight (kDa)

5.39

Isoelectric Point (pI)

48.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2324
Acc16I TGCGCA 1 cut(s) 1575
Acc36I ACCTGC 1 cut(s) 1819
AccB1I GGYRCC 1 cut(s) 364
AccBSI CCGCTC 1 cut(s) 2611
AccII CGCG 5 cut(s) 185, 210, 1333, 2035, 2451
AclWI GGATC 1 cut(s) 2175
AcoI YGGCCR 1 cut(s) 660
AcsI RAATTY 3 cut(s) 1244, 1729, 2331
AcuI CTGAAG 5 cut(s) 80, 915, 1809, 2289, 2451
AcvI CACGTG 1 cut(s) 1385
AcyI GRCGYC 1 cut(s) 1903
AfaI GTAC 6 cut(s) 483, 553, 680, 1194, 1261, 2646
AfiI CCNNNNNNNGG 5 cut(s) 162, 229, 414, 1312, 1414
AflIII ACRYGT 1 cut(s) 1438
AgsI TTSAA 9 cut(s) 381, 430, 754, 1091, 1244, 1508, 1862, 1975, 2786
AhlI ACTAGT 1 cut(s) 191
AjnI CCWGG 5 cut(s) 407, 1461, 1564, 2355, 2523
AjuI GAANNNNNNNTTGG 6 cut(s) 344, 376, 958, 990, 2568, 2600
AleI CACNNNNGTG 1 cut(s) 1415
Alw21I GWGCWC 4 cut(s) 1390, 1558, 1668, 2535
Alw26I GTCTC 6 cut(s) 985, 1200, 1321, 2105, 2365, 2661
AlwI GGATC 1 cut(s) 2175
Ama87I CYCGRG 4 cut(s) 106, 400, 420, 2176
AoxI GGCC 9 cut(s) 109, 164, 288, 324, 660, 962, 1631, 1840, 2613
ApaI GGGCCC 1 cut(s) 113
ApeKI GCWGC 6 cut(s) 55, 276, 335, 338, 1147, 2296
ApoI RAATTY 3 cut(s) 1244, 1729, 2331
AseI ATTAAT 1 cut(s) 2129
AspLEI GCGC 3 cut(s) 187, 1333, 1576
AspS9I GGNCC 8 cut(s) 109, 110, 165, 202, 324, 411, 962, 2613
AsuC2I CCSGG 3 cut(s) 107, 108, 415
AsuHPI GGTGA 2 cut(s) 1429, 2791
AsuII TTCGAA 2 cut(s) 1325, 1656
AvaI CYCGRG 4 cut(s) 106, 400, 420, 2176
AvaII GGWCC 2 cut(s) 202, 411
BaeGI GKGCMC 2 cut(s) 113, 369
BaeI ACNNNNGTAYC 2 cut(s) 1251, 1284
BalI TGGCCA 1 cut(s) 662
BanI GGYRCC 1 cut(s) 364
BanII GRGCYC 3 cut(s) 113, 421, 2535
BbrPI CACGTG 1 cut(s) 1385
BbsI GAAGAC 3 cut(s) 1640, 2093, 2219
Bbv12I GWGCWC 4 cut(s) 1390, 1558, 1668, 2535
BbvI GCAGC 6 cut(s) 67, 288, 322, 325, 1159, 2308
BccI CCATC 7 cut(s) 1110, 1637, 1709, 1819, 2009, 2635, 2783
BceAI ACGGC 3 cut(s) 110, 2102, 2600
BcgI CGANNNNNNTGC 6 cut(s) 320, 354, 1787, 1821, 2429, 2463
BciT130I CCWGG 5 cut(s) 409, 1463, 1566, 2357, 2525
BcnI CCSGG 3 cut(s) 107, 108, 415
BcoDI GTCTC 6 cut(s) 985, 1200, 1321, 2105, 2365, 2661
BcuI ACTAGT 1 cut(s) 191
BfaI CTAG 7 cut(s) 192, 1934, 1952, 2093, 2150, 2343, 2349
BfmI CTRYAG 2 cut(s) 1155, 1559
BfuAI ACCTGC 1 cut(s) 1819
Bme1390I CCNGG 8 cut(s) 107, 108, 409, 415, 1463, 1566, 2357, 2525
Bme18I GGWCC 2 cut(s) 202, 411
BmeT110I CYCGRG 4 cut(s) 106, 400, 420, 2176
BmgT120I GGNCC 8 cut(s) 109, 110, 165, 202, 324, 411, 962, 2613
BmiI GGNNCC 7 cut(s) 111, 167, 325, 366, 747, 1097, 1983
BmrFI CCNGG 8 cut(s) 107, 108, 409, 415, 1463, 1566, 2357, 2525
BmrI ACTGGG 3 cut(s) 238, 1139, 1422
BmuI ACTGGG 3 cut(s) 238, 1139, 1422
BpiI GAAGAC 3 cut(s) 1640, 2093, 2219
BplI GAGNNNNNCTC 2 cut(s) 1485, 1517
Bpu10I CCTNAGC 1 cut(s) 2555
Bpu14I TTCGAA 2 cut(s) 1325, 1656
BpuEI CTTGAG 4 cut(s) 542, 1127, 2477, 2791
BpuMI CCSGG 3 cut(s) 107, 108, 415
Bsa29I ATCGAT 1 cut(s) 2315
BsaAI YACGTR 1 cut(s) 1385
BsaHI GRCGYC 1 cut(s) 1903
BsaI GGTCTC 3 cut(s) 1321, 2365, 2661
BsaJI CCNNGG 4 cut(s) 106, 414, 965, 2106
BsaXI ACNNNNNCTCC 2 cut(s) 1792, 1822
Bsc4I CCNNNNNNNGG 5 cut(s) 162, 229, 414, 1312, 1414
Bse118I RCCGGY 1 cut(s) 2500
Bse3DI GCAATG 2 cut(s) 19, 2427
BseBI CCWGG 5 cut(s) 409, 1463, 1566, 2357, 2525
BseCI ATCGAT 1 cut(s) 2315
BseDI CCNNGG 4 cut(s) 106, 414, 965, 2106
BseGI GGATG 6 cut(s) 299, 784, 1121, 1648, 1774, 1974
BseLI CCNNNNNNNGG 5 cut(s) 162, 229, 414, 1312, 1414
BseMI GCAATG 2 cut(s) 19, 2427
BseMII CTCAG 8 cut(s) 326, 333, 573, 1332, 1382, 1602, 1649, 1869
BseRI GAGGAG 3 cut(s) 1509, 2474, 2489
BseSI GKGCMC 2 cut(s) 113, 369
BseXI GCAGC 6 cut(s) 67, 288, 322, 325, 1159, 2308
BseYI CCCAGC 1 cut(s) 121
Bsh1236I CGCG 5 cut(s) 185, 210, 1333, 2035, 2451
Bsh1285I CGRYCG 1 cut(s) 178
BshFI GGCC 9 cut(s) 111, 166, 290, 326, 662, 964, 1633, 1842, 2615
BshNI GGYRCC 1 cut(s) 364
BshVI ATCGAT 1 cut(s) 2315
BsiEI CGRYCG 1 cut(s) 178
BsiHKAI GWGCWC 4 cut(s) 1390, 1558, 1668, 2535
BsiHKCI CYCGRG 4 cut(s) 106, 400, 420, 2176
BsiSI CCGG 4 cut(s) 41, 107, 414, 2501
BslFI GGGAC 1 cut(s) 1449
BslI CCNNNNNNNGG 5 cut(s) 162, 229, 414, 1312, 1414
BsmAI GTCTC 6 cut(s) 985, 1200, 1321, 2105, 2365, 2661
BsmFI GGGAC 1 cut(s) 1449
BsmI GAATGC 3 cut(s) 1612, 1940, 2167
BsnI GGCC 9 cut(s) 111, 166, 290, 326, 662, 964, 1633, 1842, 2615
Bso31I GGTCTC 3 cut(s) 1321, 2365, 2661
BsoBI CYCGRG 4 cut(s) 106, 400, 420, 2176
Bsp119I TTCGAA 2 cut(s) 1325, 1656
Bsp120I GGGCCC 1 cut(s) 109
Bsp1286I GDGCHC 7 cut(s) 113, 369, 421, 1390, 1558, 1668, 2535
Bsp1407I TGTACA 1 cut(s) 678
Bsp143I GATC 4 cut(s) 7, 175, 1369, 2180
Bsp68I TCGCGA 1 cut(s) 2035
BspANI GGCC 9 cut(s) 111, 166, 290, 326, 662, 964, 1633, 1842, 2615
BspCNI CTCAG 8 cut(s) 325, 334, 574, 1331, 1383, 1603, 1648, 1870
BspDI ATCGAT 1 cut(s) 2315
BspFNI CGCG 5 cut(s) 185, 210, 1333, 2035, 2451
BspLI GGNNCC 7 cut(s) 111, 167, 325, 366, 747, 1097, 1983
BspMI ACCTGC 1 cut(s) 1819
BspPI GGATC 1 cut(s) 2175
BspQI GCTCTTC 1 cut(s) 2523
BspT104I TTCGAA 2 cut(s) 1325, 1656
BspT107I GGYRCC 1 cut(s) 364
BspTNI GGTCTC 3 cut(s) 1321, 2365, 2661
BsrBI CCGCTC 1 cut(s) 2611
BsrDI GCAATG 2 cut(s) 19, 2427
BsrFI RCCGGY 1 cut(s) 2500
BsrGI TGTACA 1 cut(s) 678
BssAI RCCGGY 1 cut(s) 2500
BssECI CCNNGG 4 cut(s) 106, 414, 965, 2106
BssMI GATC 4 cut(s) 7, 175, 1369, 2180
BssNI GRCGYC 1 cut(s) 1903
BssT1I CCWWGG 2 cut(s) 965, 2106
Bst2UI CCWGG 5 cut(s) 409, 1463, 1566, 2357, 2525
Bst4CI ACNGT 7 cut(s) 117, 224, 579, 1513, 1551, 1858, 2217
Bst6I CTCTTC 6 cut(s) 12, 752, 1070, 2199, 2523, 2745
BstACI GRCGYC 1 cut(s) 1903
BstAPI GCANNNNNTGC 1 cut(s) 869
BstAUI TGTACA 1 cut(s) 678
BstBAI YACGTR 1 cut(s) 1385
BstBI TTCGAA 2 cut(s) 1325, 1656
BstC8I GCNNGC 9 cut(s) 318, 397, 1001, 1152, 1335, 1840, 1901, 2301, 2613
BstENI CCTNNNNNAGG 1 cut(s) 1310
BstF5I GGATG 6 cut(s) 299, 784, 1121, 1648, 1774, 1974
BstFNI CGCG 5 cut(s) 185, 210, 1333, 2035, 2451
BstHHI GCGC 3 cut(s) 187, 1333, 1576
BstKTI GATC 4 cut(s) 10, 178, 1372, 2183
BstMAI GTCTC 6 cut(s) 985, 1200, 1321, 2105, 2365, 2661
BstMBI GATC 4 cut(s) 7, 175, 1369, 2180
BstMCI CGRYCG 1 cut(s) 178
BstNI CCWGG 5 cut(s) 409, 1463, 1566, 2357, 2525
BstNSI RCATGY 2 cut(s) 1003, 1442
BstSCI CCNGG 8 cut(s) 105, 106, 407, 413, 1461, 1564, 2355, 2523
BstSFI CTRYAG 2 cut(s) 1155, 1559
BstSLI GKGCMC 2 cut(s) 113, 369
BstUI CGCG 5 cut(s) 185, 210, 1333, 2035, 2451
BstV1I GCAGC 6 cut(s) 67, 288, 322, 325, 1159, 2308
BstV2I GAAGAC 3 cut(s) 1640, 2093, 2219
BstX2I RGATCY 1 cut(s) 2180
BstYI RGATCY 1 cut(s) 2180
Bsu15I ATCGAT 1 cut(s) 2315
BsuRI GGCC 9 cut(s) 111, 166, 290, 326, 662, 964, 1633, 1842, 2615
BsuTUI ATCGAT 1 cut(s) 2315
BtgZI GCGATG 1 cut(s) 1028
BtsCI GGATG 6 cut(s) 299, 784, 1121, 1648, 1774, 1974
BtsIMutI CAGTG 9 cut(s) 113, 575, 656, 1410, 1556, 1854, 2383, 2414, 2630
BtuMI TCGCGA 1 cut(s) 2035
BveI ACCTGC 1 cut(s) 1819
Cac8I GCNNGC 9 cut(s) 318, 397, 1001, 1152, 1335, 1840, 1901, 2301, 2613
CfoI GCGC 3 cut(s) 187, 1333, 1576
Cfr10I RCCGGY 1 cut(s) 2500
Cfr13I GGNCC 8 cut(s) 109, 110, 165, 202, 324, 411, 962, 2613
Cfr9I CCCGGG 1 cut(s) 106
ClaI ATCGAT 1 cut(s) 2315
CseI GACGC 3 cut(s) 141, 1892, 2213
CsiI ACCWGGT 1 cut(s) 2355
Csp6I GTAC 6 cut(s) 482, 552, 679, 1193, 1260, 2645
CviAII CATG 7 cut(s) 664, 912, 941, 1000, 1255, 1439, 1523
CviQI GTAC 6 cut(s) 482, 552, 679, 1193, 1260, 2645
DpnI GATC 4 cut(s) 9, 177, 1371, 2182
DpnII GATC 4 cut(s) 7, 175, 1369, 2180
DraI TTTAAA 1 cut(s) 1734
EaeI YGGCCR 1 cut(s) 660
Eam1104I CTCTTC 6 cut(s) 12, 752, 1070, 2199, 2523, 2745
EarI CTCTTC 6 cut(s) 12, 752, 1070, 2199, 2523, 2745
EciI GGCGGA 1 cut(s) 215
Ecl136II GAGCTC 1 cut(s) 2533
Eco130I CCWWGG 2 cut(s) 965, 2106
Eco147I AGGCCT 1 cut(s) 290
Eco24I GRGCYC 3 cut(s) 113, 421, 2535
Eco31I GGTCTC 3 cut(s) 1321, 2365, 2661
Eco32I GATATC 1 cut(s) 2591
Eco47I GGWCC 2 cut(s) 202, 411
Eco53kI GAGCTC 1 cut(s) 2533
Eco57I CTGAAG 5 cut(s) 80, 915, 1809, 2289, 2451
Eco72I CACGTG 1 cut(s) 1385
Eco88I CYCGRG 4 cut(s) 106, 400, 420, 2176
EcoICRI GAGCTC 1 cut(s) 2533
EcoNI CCTNNNNNAGG 1 cut(s) 1310
EcoRII CCWGG 5 cut(s) 407, 1461, 1564, 2355, 2523
EcoRV GATATC 1 cut(s) 2591
EcoT14I CCWWGG 2 cut(s) 965, 2106
EcoT22I ATGCAT 1 cut(s) 301
EcoT38I GRGCYC 3 cut(s) 113, 421, 2535
ErhI CCWWGG 2 cut(s) 965, 2106
FaeI CATG 7 cut(s) 667, 915, 944, 1003, 1258, 1442, 1526
FaqI GGGAC 1 cut(s) 1449
FatI CATG 7 cut(s) 663, 911, 940, 999, 1254, 1438, 1522
FauI CCCGC 5 cut(s) 164, 177, 313, 2565, 2604
FauNDI CATATG 1 cut(s) 802
FokI GGATG 6 cut(s) 286, 791, 1128, 1655, 1781, 1981
FriOI GRGCYC 3 cut(s) 113, 421, 2535
FspBI CTAG 7 cut(s) 192, 1934, 1952, 2093, 2150, 2343, 2349
FspI TGCGCA 1 cut(s) 1575
GlaI GCGC 3 cut(s) 186, 1332, 1575
GsaI CCCAGC 1 cut(s) 125
HaeIII GGCC 9 cut(s) 111, 166, 290, 326, 662, 964, 1633, 1842, 2615
HapII CCGG 4 cut(s) 41, 107, 414, 2501
HgaI GACGC 3 cut(s) 141, 1892, 2213
HhaI GCGC 3 cut(s) 187, 1333, 1576
Hin1I GRCGYC 1 cut(s) 1903
Hin1II CATG 7 cut(s) 667, 915, 944, 1003, 1258, 1442, 1526
Hin6I GCGC 3 cut(s) 185, 1331, 1574
HinP1I GCGC 3 cut(s) 185, 1331, 1574
HincII GTYRAC 2 cut(s) 1057, 2250
HindII GTYRAC 2 cut(s) 1057, 2250
HindIII AAGCTT 2 cut(s) 1484, 2301
HpaII CCGG 4 cut(s) 41, 107, 414, 2501
HphI GGTGA 2 cut(s) 1429, 2791
Hpy166II GTNNAC 5 cut(s) 857, 1057, 2021, 2059, 2250
Hpy8I GTNNAC 5 cut(s) 857, 1057, 2021, 2059, 2250
Hpy99I CGWCG 5 cut(s) 28, 89, 135, 244, 1908
HpyAV CCTTC 8 cut(s) 159, 273, 387, 1045, 1306, 1321, 1757, 2060
HpyCH4III ACNGT 7 cut(s) 117, 224, 579, 1513, 1551, 1858, 2217
HpyCH4IV ACGT 2 cut(s) 1262, 1384
HpySE526I ACGT 2 cut(s) 1262, 1384
Hsp92I GRCGYC 1 cut(s) 1903
Hsp92II CATG 7 cut(s) 667, 915, 944, 1003, 1258, 1442, 1526
HspAI GCGC 3 cut(s) 185, 1331, 1574
Kzo9I GATC 4 cut(s) 7, 175, 1369, 2180
LguI GCTCTTC 1 cut(s) 2523
LmnI GCTCC 2 cut(s) 1813, 2461
Lsp1109I GCAGC 6 cut(s) 67, 288, 322, 325, 1159, 2308
MabI ACCWGGT 1 cut(s) 2355
MaeI CTAG 7 cut(s) 192, 1934, 1952, 2093, 2150, 2343, 2349
MaeII ACGT 2 cut(s) 1262, 1384
MaeIII GTNAC 4 cut(s) 224, 1457, 1907, 2711
MalI GATC 4 cut(s) 9, 177, 1371, 2182
MbiI CCGCTC 1 cut(s) 2611
MboI GATC 4 cut(s) 7, 175, 1369, 2180
MflI RGATCY 1 cut(s) 2180
MhlI GDGCHC 7 cut(s) 113, 369, 421, 1390, 1558, 1668, 2535
MlsI TGGCCA 1 cut(s) 662
MluNI TGGCCA 1 cut(s) 662
MlyI GAGTC 5 cut(s) 29, 1052, 2107, 2150, 2168
MmeI TCCRAC 3 cut(s) 617, 1471, 1620
Mox20I TGGCCA 1 cut(s) 662
Mph1103I ATGCAT 1 cut(s) 301
MscI TGGCCA 1 cut(s) 662
MseI TTAA 4 cut(s) 357, 1541, 1733, 2129
MslI CAYNNNNRTG 2 cut(s) 1415, 2664
Msp20I TGGCCA 1 cut(s) 662
MspA1I CMGCKG 1 cut(s) 58
MspI CCGG 4 cut(s) 41, 107, 414, 2501
MspR9I CCNGG 8 cut(s) 107, 108, 409, 415, 1463, 1566, 2357, 2525
Mva1269I GAATGC 3 cut(s) 1612, 1940, 2167
MvaI CCWGG 5 cut(s) 409, 1463, 1566, 2357, 2525
MvnI CGCG 5 cut(s) 185, 210, 1333, 2035, 2451
NciI CCSGG 3 cut(s) 107, 108, 415
NdeI CATATG 1 cut(s) 802
NdeII GATC 4 cut(s) 7, 175, 1369, 2180
NlaIII CATG 7 cut(s) 667, 915, 944, 1003, 1258, 1442, 1526
NlaIV GGNNCC 7 cut(s) 111, 167, 325, 366, 747, 1097, 1983
NmuCI GTSAC 3 cut(s) 224, 1457, 1907
NruI TCGCGA 1 cut(s) 2035
NsbI TGCGCA 1 cut(s) 1575
NsiI ATGCAT 1 cut(s) 301
NspI RCATGY 2 cut(s) 1003, 1442
NspV TTCGAA 2 cut(s) 1325, 1656
OliI CACNNNNGTG 1 cut(s) 1415
PaeI GCATGC 1 cut(s) 1003
PaeR7I CTCGAG 2 cut(s) 420, 2176
PceI AGGCCT 1 cut(s) 290
PciI ACATGT 1 cut(s) 1438
PciSI GCTCTTC 1 cut(s) 2523
PctI GAATGC 3 cut(s) 1612, 1940, 2167
PfeI GAWTC 9 cut(s) 259, 589, 929, 1698, 2120, 2144, 2284, 2339, 2373
PflFI GACNNNGTC 1 cut(s) 230
PfoI TCCNGGA 1 cut(s) 407
Ple19I CGATCG 1 cut(s) 178
PleI GAGTC 5 cut(s) 28, 1052, 2106, 2150, 2168
PmaCI CACGTG 1 cut(s) 1385
PmlI CACGTG 1 cut(s) 1385
PpsI GAGTC 5 cut(s) 28, 1052, 2106, 2150, 2168
Ppu21I YACGTR 1 cut(s) 1385
PscI ACATGT 1 cut(s) 1438
PshBI ATTAAT 1 cut(s) 2129
PsiI TTATAA 1 cut(s) 2324
Psp124BI GAGCTC 1 cut(s) 2535
Psp6I CCWGG 5 cut(s) 407, 1461, 1564, 2355, 2523
PspCI CACGTG 1 cut(s) 1385
PspFI CCCAGC 1 cut(s) 121
PspGI CCWGG 5 cut(s) 407, 1461, 1564, 2355, 2523
PspN4I GGNNCC 7 cut(s) 111, 167, 325, 366, 747, 1097, 1983
PspOMI GGGCCC 1 cut(s) 109
PspPI GGNCC 8 cut(s) 109, 110, 165, 202, 324, 411, 962, 2613
PsrI GAACNNNNNNTAC 2 cut(s) 2760, 2792
PsuI RGATCY 1 cut(s) 2180
PsyI GACNNNGTC 1 cut(s) 230
PvuI CGATCG 1 cut(s) 178
PvuII CAGCTG 1 cut(s) 58
RruI TCGCGA 1 cut(s) 2035
RsaI GTAC 6 cut(s) 483, 553, 680, 1194, 1261, 2646
RsaNI GTAC 6 cut(s) 482, 552, 679, 1193, 1260, 2645
RseI CAYNNNNRTG 2 cut(s) 1415, 2664
SacI GAGCTC 1 cut(s) 2535
SapI GCTCTTC 1 cut(s) 2523
SaqAI TTAA 4 cut(s) 357, 1541, 1733, 2129
Sau3AI GATC 4 cut(s) 7, 175, 1369, 2180
Sau96I GGNCC 8 cut(s) 109, 110, 165, 202, 324, 411, 962, 2613
SchI GAGTC 5 cut(s) 29, 1052, 2107, 2150, 2168
ScrFI CCNGG 8 cut(s) 107, 108, 409, 415, 1463, 1566, 2357, 2525
SduI GDGCHC 7 cut(s) 113, 369, 421, 1390, 1558, 1668, 2535
SexAI ACCWGGT 1 cut(s) 2355
SfcI CTRYAG 2 cut(s) 1155, 1559
Sfr274I CTCGAG 2 cut(s) 420, 2176
SfuI TTCGAA 2 cut(s) 1325, 1656
SinI GGWCC 2 cut(s) 202, 411
SlaI CTCGAG 2 cut(s) 420, 2176
SmaI CCCGGG 1 cut(s) 108
SmiI ATTTAAAT 1 cut(s) 1734
SmiMI CAYNNNNRTG 2 cut(s) 1415, 2664
SmlI CTYRAG 6 cut(s) 420, 521, 1142, 2176, 2456, 2806
SmoI CTYRAG 6 cut(s) 420, 521, 1142, 2176, 2456, 2806
SpeI ACTAGT 1 cut(s) 191
SphI GCATGC 1 cut(s) 1003
SseBI AGGCCT 1 cut(s) 290
SspI AATATT 2 cut(s) 565, 837
SspMI CTAG 7 cut(s) 192, 1934, 1952, 2093, 2150, 2343, 2349
SstI GAGCTC 1 cut(s) 2535
StuI AGGCCT 1 cut(s) 290
StyD4I CCNGG 8 cut(s) 105, 106, 407, 413, 1461, 1564, 2355, 2523
StyI CCWWGG 2 cut(s) 965, 2106
SwaI ATTTAAAT 1 cut(s) 1734
TaaI ACNGT 7 cut(s) 117, 224, 579, 1513, 1551, 1858, 2217
TaiI ACGT 2 cut(s) 1265, 1387
TaqI TCGA 7 cut(s) 26, 130, 421, 1325, 1656, 2177, 2315
TatI WGTACW 2 cut(s) 678, 1192
TauI GCSGC 4 cut(s) 84, 200, 505, 1712
TfiI GAWTC 9 cut(s) 259, 589, 929, 1698, 2120, 2144, 2284, 2339, 2373
Tru1I TTAA 4 cut(s) 357, 1541, 1733, 2129
Tru9I TTAA 4 cut(s) 357, 1541, 1733, 2129
TscAI CASTG 9 cut(s) 120, 582, 663, 1417, 1556, 1861, 2383, 2421, 2637
TseFI GTSAC 3 cut(s) 224, 1457, 1907
TseI GCWGC 6 cut(s) 55, 276, 335, 338, 1147, 2296
Tsp45I GTSAC 3 cut(s) 224, 1457, 1907
TspGWI ACGGA 3 cut(s) 85, 228, 2662
TspMI CCCGGG 1 cut(s) 106
TspRI CASTG 9 cut(s) 120, 582, 663, 1417, 1556, 1861, 2383, 2421, 2637
Tth111I GACNNNGTC 1 cut(s) 230
VpaK11BI GGWCC 2 cut(s) 202, 411
VspI ATTAAT 1 cut(s) 2129
XagI CCTNNNNNAGG 1 cut(s) 1310
XapI RAATTY 3 cut(s) 1244, 1729, 2331
XbaI TCTAGA 2 cut(s) 1933, 2092
XceI RCATGY 2 cut(s) 1003, 1442
XhoI CTCGAG 2 cut(s) 420, 2176
XmaI CCCGGG 1 cut(s) 106
XspI CTAG 7 cut(s) 192, 1934, 1952, 2093, 2150, 2343, 2349
Zsp2I ATGCAT 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.