FvH4_6g26000

Ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001000
Physical Location & Seq
Forward (+)
19883404 .. 19894161
10758 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g26000.t15

Sequence Viewer

Length: 2460 bp
ATGGTCGATCGGAATGAGGAGTCGTTGCCGCAGCTGAAGAAATCACGAACTATAACCGGCGACGAGGCAACCCCAATCTTCCCGGGCCCACTGTTCCCAGCGGTTCGACGCAACGCTTCGGACCAGCGCGACTTCTCGGAGCGTGACTGGGTCTTTCCGTCGTTCGTGCTTCCGCAGAATCCTTCGAAACGAAGCAGCAGCAACAAGCACCGCGCCGCCTCCTCCTCCGATGCATCCGTCACCTCGCCTCAGCTTGCTGCTGCTGCTGAGAGTAAGAAAATTAAGTTGGTGACCCCGAGCGAGTTGACTCGCCCGAGTTCGCGGACCCGGACTCGAGATTTGAAACCTTTTTCTTTGTTGTTATTTTTGCTCACTTTCATTTGTATATTATCTGTACCTTATGCAGTTTATCTGCGGCAAAGAGTTGCAAAGCTTGAGGAAATTTGTATGGATAAAAATATTGGTACATATGGCAATATTGAGGCTTCGCTGTCTGATAACAATCCGACAATTTTGAGTTTTCCTAATGCCGAAAGAAGAACTGTTGTTTTGTATTCTGTGGTCTTTACCCTAGCCATGCCAATTGTGTTGTACAAACATCTTGATTATTTTTCCCATTTGACGAGCCTTTCAAGAAAGAAGAATAACAAAGAGGAGGTTCCCTTGAAGAAGAGGATAGCCTATATGGTGGATGTGTGCTTTTCTGTATATCCATATGCCAAACTACTGGCACTTCTCTTCGCAACAATATTACTAATAGGGTTTGGTGGATTGGCATTGTATGCTGTCAATAAAAATAGCTTTGCTGAAGCCCTCTGGCTTTCATGGACTTTCGTTGCCGATTCTGGAAACCATGCTGATACAGAGGGCATTGGGCCAAGGATAGTTTCTGTCTCTATCAGTTCAGGAGGCATGCTGATATTTGCCATGATGCTTGGGCTTGTTTCTGATGCTATATCAGAGAAGGTTGACTCACTAAGGAAAGGGAAGAGCGAAGTCATTGAAAGGAACCACATACTCATTCTTGGATGGAGTGATAAACTGGGTTCGCTTCTCAAGCAGCTTGCTATAGCAAACAAGAGTGTTGGTGGTGGTGTTGTTGTTGTACTTGCTGAAAGAGACAAGGAGGAAATGGAGATTGATATTGCAAAACTTGAATTTGACTTCATGGGTACGTCTGTTATATGCAGAAGTGGCAGTCCTCTTATTTTTGCTGACCTAAAGAAGGTCTCAGTTTCAAAGGCGCGTGCTATCATTGTATTGGCATCAGATGAAAACGCAGATCAGAGTGATGCACGTGCTCTGAGGGTTGTTCTCAGCCTCACTGGGGTGAAAGAGGGTTTAAGGGGACATGTTGTTGTAGAGATGAGTGACCTTGATAATGAGCCTCTGGTGAAGCTTGTTGGAGGAGAACTCATTGAAACTGTAGTGGCACATGATGTGATTGGGCGGTTAATGATACAGTGTGCTCTACAGCCTGGTCTTGCGCAGATATGGGAGGATATATTGGGATTTGAGAATGCTGAGTTTTACATCAAGAGGTGGCCTCAGTTGGATGGTCTTCGTTTTGAAAATGTGCTTATTTCATTTCCTGATGCAATTCCCTGTGGGATCAAGGTGGCCGCAGATGGTGGAAAGATAAACTTAAATCCAGATGATAAATATGTTATAAAAGAAGGGGACGAAGTTCTTGTTATAGCTGAAGATGACGACACTTATGCTCCATCTGCCATTCCAGAGGTATGTGCTGGCCTTTGTCCAAACACTGTTGAACCTCCCAAATACCCTGAGAAGATATTGTTCTGTGGCTGGCGTCGTGACATAGACGATATGATACTGGTTCTAGAGGCATTCTTGCCTCTAGGTTCGGAACTTTGGATGTTTAATGAGGTTCCAGAAAAAGAAAGAGAGAAGAAACTTACAGATGGTGGACTTGAGATTTCTCGATTGGAGAACATAAAACTCGTTCACAGAGAAGGAAATGCTGTGATAAGACGGCATCTAGAGAGTCTTCCCTTGGAGACTTTTGATTCTATATTAATTCTTGCAGATGAATCACTAGAGGACTCTGTTGTGCATTCTGACTCTAGATCCCTTGCCACTCTTCTCCTTATACGAGATATACAGTCAAAGCGTCTTCCTTACAAAGAAACAAAGCCAACTTCCTTACGGTTATCTGCATTTTCGCATAGCTCTTGGATTTGTGAAATGCAGCAAGCTTCTGACAAATCGATAATAATAAGTGAAATTCTGGATTCTAGGACTAGAAACCTGGTCTCTGTTTCCAGAATCAGTGATTATGTGCTGTCAAATGAACTTGTTAGTATGGCTCTGGCAATGGTTGCTGAAGATAAGCAGATAAATCGTGTTCTTGAAGAGCTATTTGCGGAGGAGGTACTCTCCAGTCTTTCTGGAATTGATTTTATACGAACATGCTGTGGTAGTTTATATGTGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Castor_Poll_mid

Protein Analysis

820

Amino Acids

90.88

Weight (kDa)

5.43

Isoelectric Point (pI)

47.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Slo-like_RCK PF22614 187 - 291 2e-07 Calcium-activated potassium channel slowpoke-like RCK domain
Castor_Poll_mid PF06241 330 - 428 6e-43 Castor and Pollux, part of voltage-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1670
Acc16I TGCGCA 1 cut(s) 1488
AccII CGCG 4 cut(s) 129, 213, 322, 1246
AclWI GGATC 2 cut(s) 1619, 2085
AcoI YGGCCR 1 cut(s) 1620
AcsI RAATTY 3 cut(s) 441, 1157, 2247
AcuI CTGAAG 4 cut(s) 56, 828, 1722, 2367
AcvI CACGTG 1 cut(s) 1298
AcyI GRCGYC 1 cut(s) 1813
AfaI GTAC 6 cut(s) 396, 466, 593, 1107, 1174, 2397
AfiI CCNNNNNNNGG 2 cut(s) 1225, 1327
AflIII ACRYGT 1 cut(s) 1351
AjnI CCWGG 2 cut(s) 1477, 2271
AjuI GAANNNNNNNTTGG 4 cut(s) 269, 301, 871, 903
AleI CACNNNNGTG 1 cut(s) 1328
Alw21I GWGCWC 2 cut(s) 1303, 1471
Alw26I GTCTC 5 cut(s) 898, 1113, 1234, 2015, 2281
AlwI GGATC 2 cut(s) 1619, 2085
Ama87I CYCGRG 4 cut(s) 82, 295, 313, 333
AoxI GGCC 5 cut(s) 85, 875, 1544, 1620, 1750
ApaI GGGCCC 1 cut(s) 89
ApeKI GCWGC 8 cut(s) 31, 195, 198, 257, 260, 263, 1060, 2212
ApoI RAATTY 3 cut(s) 441, 1157, 2247
ArsI GACNNNNNNTTYG 2 cut(s) 322, 354
AseI ATTAAT 1 cut(s) 2039
AspLEI GCGC 4 cut(s) 129, 215, 1246, 1489
AspS9I GGNCC 5 cut(s) 85, 86, 121, 324, 875
AsuC2I CCSGG 3 cut(s) 83, 84, 328
AsuHPI GGTGA 4 cut(s) 232, 301, 1342, 1405
AsuII TTCGAA 1 cut(s) 185
AvaI CYCGRG 4 cut(s) 82, 295, 313, 333
AvaII GGWCC 2 cut(s) 121, 324
BaeGI GKGCMC 1 cut(s) 89
BaeI ACNNNNGTAYC 2 cut(s) 1164, 1197
BanII GRGCYC 1 cut(s) 89
BbrPI CACGTG 1 cut(s) 1298
BbsI GAAGAC 3 cut(s) 1553, 2003, 2129
Bbv12I GWGCWC 2 cut(s) 1303, 1471
BbvCI CCTCAGC 1 cut(s) 249
BbvI GCAGC 8 cut(s) 43, 207, 210, 244, 247, 250, 1072, 2224
BccI CCATC 5 cut(s) 1023, 1550, 1622, 1732, 1919
BceAI ACGGC 1 cut(s) 2012
BcgI CGANNNNNNTGC 4 cut(s) 1700, 1734, 2345, 2379
BciT130I CCWGG 2 cut(s) 1479, 2273
BcnI CCSGG 3 cut(s) 83, 84, 328
BcoDI GTCTC 5 cut(s) 898, 1113, 1234, 2015, 2281
BfaI CTAG 8 cut(s) 572, 1844, 1862, 2003, 2060, 2088, 2259, 2265
BfmI CTRYAG 3 cut(s) 1068, 1425, 1472
Bme1390I CCNGG 5 cut(s) 83, 84, 328, 1479, 2273
Bme18I GGWCC 2 cut(s) 121, 324
BmeT110I CYCGRG 4 cut(s) 82, 295, 313, 333
BmgT120I GGNCC 5 cut(s) 85, 86, 121, 324, 875
BmiI GGNNCC 5 cut(s) 87, 326, 660, 1010, 1893
BmrFI CCNGG 5 cut(s) 83, 84, 328, 1479, 2273
BmrI ACTGGG 3 cut(s) 157, 1052, 1335
BmsI GCATC 8 cut(s) 220, 242, 921, 940, 1274, 1282, 1585, 2008
BmuI ACTGGG 3 cut(s) 157, 1052, 1335
BpiI GAAGAC 3 cut(s) 1553, 2003, 2129
BplI GAGNNNNNCTC 6 cut(s) 1398, 1430, 1531, 1563, 2384, 2416
BpmI CTGGAG 1 cut(s) 2386
Bpu10I CCTNAGC 1 cut(s) 249
Bpu14I TTCGAA 1 cut(s) 185
BpuEI CTTGAG 3 cut(s) 455, 1040, 1955
BpuMI CCSGG 3 cut(s) 83, 84, 328
Bsa29I ATCGAT 1 cut(s) 2231
BsaAI YACGTR 1 cut(s) 1298
BsaBI GATNNNNATC 1 cut(s) 501
BsaHI GRCGYC 1 cut(s) 1813
BsaI GGTCTC 2 cut(s) 1234, 2281
BsaJI CCNNGG 3 cut(s) 82, 878, 2016
BsaXI ACNNNNNCTCC 2 cut(s) 1705, 1735
Bsc4I CCNNNNNNNGG 2 cut(s) 1225, 1327
Bse118I RCCGGY 1 cut(s) 56
Bse1I ACTGG 6 cut(s) 152, 732, 1047, 1330, 1842, 2403
Bse3DI GCAATG 1 cut(s) 2343
Bse8I GATNNNNATC 1 cut(s) 501
BseBI CCWGG 2 cut(s) 1479, 2273
BseCI ATCGAT 1 cut(s) 2231
BseDI CCNNGG 3 cut(s) 82, 878, 2016
BseGI GGATG 5 cut(s) 233, 697, 1034, 1561, 1884
BseJI GATNNNNATC 1 cut(s) 501
BseLI CCNNNNNNNGG 2 cut(s) 1225, 1327
BseMI GCAATG 1 cut(s) 2343
BseMII CTCAG 8 cut(s) 258, 263, 1245, 1295, 1330, 1515, 1562, 1779
BseNI ACTGG 6 cut(s) 152, 732, 1047, 1330, 1842, 2403
BseRI GAGGAG 6 cut(s) 32, 211, 214, 668, 1422, 2405
BseSI GKGCMC 1 cut(s) 89
BseXI GCAGC 8 cut(s) 43, 207, 210, 244, 247, 250, 1072, 2224
BseYI CCCAGC 1 cut(s) 97
Bsh1236I CGCG 4 cut(s) 129, 213, 322, 1246
Bsh1285I CGRYCG 1 cut(s) 10
BshFI GGCC 5 cut(s) 87, 877, 1546, 1622, 1752
BshVI ATCGAT 1 cut(s) 2231
BsiEI CGRYCG 1 cut(s) 10
BsiHKAI GWGCWC 2 cut(s) 1303, 1471
BsiHKCI CYCGRG 4 cut(s) 82, 295, 313, 333
BsiSI CCGG 3 cut(s) 57, 83, 328
BslFI GGGAC 2 cut(s) 1362, 1694
BslI CCNNNNNNNGG 2 cut(s) 1225, 1327
BsmAI GTCTC 5 cut(s) 898, 1113, 1234, 2015, 2281
BsmFI GGGAC 2 cut(s) 1362, 1694
BsmI GAATGC 3 cut(s) 1525, 1850, 2077
BsnI GGCC 5 cut(s) 87, 877, 1546, 1622, 1752
Bso31I GGTCTC 2 cut(s) 1234, 2281
BsoBI CYCGRG 4 cut(s) 82, 295, 313, 333
Bsp119I TTCGAA 1 cut(s) 185
Bsp120I GGGCCC 1 cut(s) 85
Bsp1286I GDGCHC 3 cut(s) 89, 1303, 1471
Bsp1407I TGTACA 1 cut(s) 591
Bsp143I GATC 4 cut(s) 7, 1282, 1611, 2090
BspANI GGCC 5 cut(s) 87, 877, 1546, 1622, 1752
BspCNI CTCAG 8 cut(s) 259, 262, 1244, 1296, 1329, 1516, 1561, 1780
BspDI ATCGAT 1 cut(s) 2231
BspFNI CGCG 4 cut(s) 129, 213, 322, 1246
BspLI GGNNCC 5 cut(s) 87, 326, 660, 1010, 1893
BspPI GGATC 2 cut(s) 1619, 2085
BspQI GCTCTTC 2 cut(s) 983, 2370
BspT104I TTCGAA 1 cut(s) 185
BspTNI GGTCTC 2 cut(s) 1234, 2281
BsrDI GCAATG 1 cut(s) 2343
BsrFI RCCGGY 1 cut(s) 56
BsrGI TGTACA 1 cut(s) 591
BsrI ACTGG 6 cut(s) 152, 732, 1047, 1330, 1842, 2403
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 3 cut(s) 82, 878, 2016
BssMI GATC 4 cut(s) 7, 1282, 1611, 2090
BssNI GRCGYC 1 cut(s) 1813
BssT1I CCWWGG 2 cut(s) 878, 2016
Bst2UI CCWGG 2 cut(s) 1479, 2273
Bst4CI ACNGT 7 cut(s) 93, 544, 1426, 1464, 1768, 2127, 2172
Bst6I CTCTTC 5 cut(s) 665, 743, 983, 2109, 2370
BstACI GRCGYC 1 cut(s) 1813
BstAPI GCANNNNNTGC 2 cut(s) 782, 2342
BstAUI TGTACA 1 cut(s) 591
BstBAI YACGTR 1 cut(s) 1298
BstBI TTCGAA 1 cut(s) 185
BstC8I GCNNGC 7 cut(s) 255, 914, 1065, 1248, 1750, 1811, 2217
BstDEI CTNAG 9 cut(s) 249, 267, 977, 1231, 1304, 1316, 1524, 1548, 1788
BstEII GGTNACC 1 cut(s) 289
BstENI CCTNNNNNAGG 1 cut(s) 1223
BstF5I GGATG 5 cut(s) 233, 697, 1034, 1561, 1884
BstFNI CGCG 4 cut(s) 129, 213, 322, 1246
BstHHI GCGC 4 cut(s) 129, 215, 1246, 1489
BstKTI GATC 4 cut(s) 10, 1285, 1614, 2093
BstMAI GTCTC 5 cut(s) 898, 1113, 1234, 2015, 2281
BstMBI GATC 4 cut(s) 7, 1282, 1611, 2090
BstMCI CGRYCG 1 cut(s) 10
BstMWI GCNNNNNNNGC 6 cut(s) 263, 782, 1057, 1194, 1727, 2342
BstNI CCWGG 2 cut(s) 1479, 2273
BstNSI RCATGY 3 cut(s) 916, 1355, 2436
BstPI GGTNACC 1 cut(s) 289
BstSCI CCNGG 5 cut(s) 81, 82, 326, 1477, 2271
BstSFI CTRYAG 3 cut(s) 1068, 1425, 1472
BstSLI GKGCMC 1 cut(s) 89
BstUI CGCG 4 cut(s) 129, 213, 322, 1246
BstV1I GCAGC 8 cut(s) 43, 207, 210, 244, 247, 250, 1072, 2224
BstV2I GAAGAC 3 cut(s) 1553, 2003, 2129
BstX2I RGATCY 1 cut(s) 2090
BstXI CCANNNNNNTGG 1 cut(s) 727
BstYI RGATCY 1 cut(s) 2090
Bsu15I ATCGAT 1 cut(s) 2231
BsuRI GGCC 5 cut(s) 87, 877, 1546, 1622, 1752
BsuTUI ATCGAT 1 cut(s) 2231
BtsCI GGATG 5 cut(s) 233, 697, 1034, 1561, 1884
BtsIMutI CAGTG 5 cut(s) 89, 1323, 1469, 1764, 2299
Cac8I GCNNGC 7 cut(s) 255, 914, 1065, 1248, 1750, 1811, 2217
CfoI GCGC 4 cut(s) 129, 215, 1246, 1489
Cfr10I RCCGGY 1 cut(s) 56
Cfr13I GGNCC 5 cut(s) 85, 86, 121, 324, 875
Cfr9I CCCGGG 1 cut(s) 82
ClaI ATCGAT 1 cut(s) 2231
CseI GACGC 3 cut(s) 117, 1802, 2123
CsiI ACCWGGT 1 cut(s) 2271
Csp6I GTAC 6 cut(s) 395, 465, 592, 1106, 1173, 2396
CviAII CATG 9 cut(s) 577, 825, 854, 913, 928, 1168, 1352, 1436, 2433
CviQI GTAC 6 cut(s) 395, 465, 592, 1106, 1173, 2396
DdeI CTNAG 9 cut(s) 249, 267, 977, 1231, 1304, 1316, 1524, 1548, 1788
DpnI GATC 4 cut(s) 9, 1284, 1613, 2092
DpnII GATC 4 cut(s) 7, 1282, 1611, 2090
EaeI YGGCCR 1 cut(s) 1620
Eam1104I CTCTTC 5 cut(s) 665, 743, 983, 2109, 2370
EarI CTCTTC 5 cut(s) 665, 743, 983, 2109, 2370
Eco130I CCWWGG 2 cut(s) 878, 2016
Eco24I GRGCYC 1 cut(s) 89
Eco31I GGTCTC 2 cut(s) 1234, 2281
Eco47I GGWCC 2 cut(s) 121, 324
Eco57I CTGAAG 4 cut(s) 56, 828, 1722, 2367
Eco72I CACGTG 1 cut(s) 1298
Eco88I CYCGRG 4 cut(s) 82, 295, 313, 333
Eco91I GGTNACC 1 cut(s) 289
EcoNI CCTNNNNNAGG 1 cut(s) 1223
EcoO65I GGTNACC 1 cut(s) 289
EcoRII CCWGG 2 cut(s) 1477, 2271
EcoT14I CCWWGG 2 cut(s) 878, 2016
EcoT22I ATGCAT 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 89
ErhI CCWWGG 2 cut(s) 878, 2016
FaeI CATG 9 cut(s) 580, 828, 857, 916, 931, 1171, 1355, 1439, 2436
FalI AAGNNNNNCTT 2 cut(s) 1628, 1660
FaqI GGGAC 2 cut(s) 1362, 1694
FatI CATG 9 cut(s) 576, 824, 853, 912, 927, 1167, 1351, 1435, 2432
FauNDI CATATG 2 cut(s) 469, 715
FokI GGATG 5 cut(s) 220, 704, 1041, 1568, 1891
FriOI GRGCYC 1 cut(s) 89
FspBI CTAG 8 cut(s) 572, 1844, 1862, 2003, 2060, 2088, 2259, 2265
FspI TGCGCA 1 cut(s) 1488
GlaI GCGC 4 cut(s) 128, 214, 1245, 1488
GsaI CCCAGC 1 cut(s) 101
GsuI CTGGAG 1 cut(s) 2386
HaeIII GGCC 5 cut(s) 87, 877, 1546, 1622, 1752
HapII CCGG 3 cut(s) 57, 83, 328
HgaI GACGC 3 cut(s) 117, 1802, 2123
HhaI GCGC 4 cut(s) 129, 215, 1246, 1489
Hin1I GRCGYC 1 cut(s) 1813
Hin1II CATG 9 cut(s) 580, 828, 857, 916, 931, 1171, 1355, 1439, 2436
Hin6I GCGC 4 cut(s) 127, 213, 1244, 1487
HinP1I GCGC 4 cut(s) 127, 213, 1244, 1487
HincII GTYRAC 2 cut(s) 306, 970
HindII GTYRAC 2 cut(s) 306, 970
HindIII AAGCTT 3 cut(s) 431, 1397, 2217
HpaII CCGG 3 cut(s) 57, 83, 328
HphI GGTGA 4 cut(s) 232, 301, 1342, 1405
Hpy166II GTNNAC 4 cut(s) 306, 970, 1931, 1969
Hpy8I GTNNAC 4 cut(s) 306, 970, 1931, 1969
Hpy99I CGWCG 4 cut(s) 65, 111, 163, 1818
HpyAV CCTTC 5 cut(s) 192, 958, 1219, 1670, 1970
HpyCH4III ACNGT 7 cut(s) 93, 544, 1426, 1464, 1768, 2127, 2172
HpyCH4IV ACGT 2 cut(s) 1175, 1297
HpyF10VI GCNNNNNNNGC 6 cut(s) 263, 782, 1057, 1194, 1727, 2342
HpyF3I CTNAG 9 cut(s) 249, 267, 977, 1231, 1304, 1316, 1524, 1548, 1788
HpySE526I ACGT 2 cut(s) 1175, 1297
Hsp92I GRCGYC 1 cut(s) 1813
Hsp92II CATG 9 cut(s) 580, 828, 857, 916, 931, 1171, 1355, 1439, 2436
HspAI GCGC 4 cut(s) 127, 213, 1244, 1487
Kzo9I GATC 4 cut(s) 7, 1282, 1611, 2090
LguI GCTCTTC 2 cut(s) 983, 2370
LmnI GCTCC 2 cut(s) 139, 1726
Lsp1109I GCAGC 8 cut(s) 43, 207, 210, 244, 247, 250, 1072, 2224
LweI GCATC 8 cut(s) 220, 242, 921, 940, 1274, 1282, 1585, 2008
MabI ACCWGGT 1 cut(s) 2271
MaeI CTAG 8 cut(s) 572, 1844, 1862, 2003, 2060, 2088, 2259, 2265
MaeII ACGT 2 cut(s) 1175, 1297
MaeIII GTNAC 5 cut(s) 143, 238, 289, 1370, 1817
MalI GATC 4 cut(s) 9, 1284, 1613, 2092
MboI GATC 4 cut(s) 7, 1282, 1611, 2090
MfeI CAATTG 1 cut(s) 582
MflI RGATCY 1 cut(s) 2090
MhlI GDGCHC 3 cut(s) 89, 1303, 1471
MluCI AATT 9 cut(s) 279, 441, 510, 582, 1157, 1599, 2040, 2247, 2415
MlyI GAGTC 7 cut(s) 29, 301, 325, 965, 2017, 2060, 2078
MmeI TCCRAC 3 cut(s) 530, 1384, 1533
Mph1103I ATGCAT 1 cut(s) 235
MseI TTAA 6 cut(s) 282, 1343, 1454, 1646, 1884, 2039
MslI CAYNNNNRTG 1 cut(s) 1328
MspA1I CMGCKG 2 cut(s) 34, 101
MspI CCGG 3 cut(s) 57, 83, 328
MspR9I CCNGG 5 cut(s) 83, 84, 328, 1479, 2273
MunI CAATTG 1 cut(s) 582
Mva1269I GAATGC 3 cut(s) 1525, 1850, 2077
MvaI CCWGG 2 cut(s) 1479, 2273
MvnI CGCG 4 cut(s) 129, 213, 322, 1246
MwoI GCNNNNNNNGC 6 cut(s) 263, 782, 1057, 1194, 1727, 2342
NciI CCSGG 3 cut(s) 83, 84, 328
NdeI CATATG 2 cut(s) 469, 715
NdeII GATC 4 cut(s) 7, 1282, 1611, 2090
NlaIII CATG 9 cut(s) 580, 828, 857, 916, 931, 1171, 1355, 1439, 2436
NlaIV GGNNCC 5 cut(s) 87, 326, 660, 1010, 1893
NmuCI GTSAC 5 cut(s) 143, 238, 289, 1370, 1817
NsbI TGCGCA 1 cut(s) 1488
NsiI ATGCAT 1 cut(s) 235
NspI RCATGY 3 cut(s) 916, 1355, 2436
NspV TTCGAA 1 cut(s) 185
OliI CACNNNNGTG 1 cut(s) 1328
PaeI GCATGC 1 cut(s) 916
PaeR7I CTCGAG 1 cut(s) 333
PciI ACATGT 1 cut(s) 1351
PciSI GCTCTTC 2 cut(s) 983, 2370
PctI GAATGC 3 cut(s) 1525, 1850, 2077
PfeI GAWTC 6 cut(s) 178, 842, 2030, 2054, 2255, 2289
PflFI GACNNNGTC 1 cut(s) 149
Ple19I CGATCG 1 cut(s) 10
PleI GAGTC 7 cut(s) 28, 301, 325, 965, 2016, 2060, 2078
PmaCI CACGTG 1 cut(s) 1298
PmlI CACGTG 1 cut(s) 1298
PpsI GAGTC 7 cut(s) 28, 301, 325, 965, 2016, 2060, 2078
Ppu21I YACGTR 1 cut(s) 1298
PscI ACATGT 1 cut(s) 1351
PshBI ATTAAT 1 cut(s) 2039
PsiI TTATAA 1 cut(s) 1670
Psp6I CCWGG 2 cut(s) 1477, 2271
PspCI CACGTG 1 cut(s) 1298
PspEI GGTNACC 1 cut(s) 289
PspFI CCCAGC 1 cut(s) 97
PspGI CCWGG 2 cut(s) 1477, 2271
PspN4I GGNNCC 5 cut(s) 87, 326, 660, 1010, 1893
PspOMI GGGCCC 1 cut(s) 85
PspPI GGNCC 5 cut(s) 85, 86, 121, 324, 875
PsuI RGATCY 1 cut(s) 2090
PsyI GACNNNGTC 1 cut(s) 149
PvuI CGATCG 1 cut(s) 10
PvuII CAGCTG 1 cut(s) 34
RsaI GTAC 6 cut(s) 396, 466, 593, 1107, 1174, 2397
RsaNI GTAC 6 cut(s) 395, 465, 592, 1106, 1173, 2396
RseI CAYNNNNRTG 1 cut(s) 1328
SapI GCTCTTC 2 cut(s) 983, 2370
SaqAI TTAA 6 cut(s) 282, 1343, 1454, 1646, 1884, 2039
Sau3AI GATC 4 cut(s) 7, 1282, 1611, 2090
Sau96I GGNCC 5 cut(s) 85, 86, 121, 324, 875
SchI GAGTC 7 cut(s) 29, 301, 325, 965, 2017, 2060, 2078
ScrFI CCNGG 5 cut(s) 83, 84, 328, 1479, 2273
SduI GDGCHC 3 cut(s) 89, 1303, 1471
SexAI ACCWGGT 1 cut(s) 2271
SfaNI GCATC 8 cut(s) 220, 242, 921, 940, 1274, 1282, 1585, 2008
SfcI CTRYAG 3 cut(s) 1068, 1425, 1472
Sfr274I CTCGAG 1 cut(s) 333
SfuI TTCGAA 1 cut(s) 185
SinI GGWCC 2 cut(s) 121, 324
SlaI CTCGAG 1 cut(s) 333
SmaI CCCGGG 1 cut(s) 84
SmiMI CAYNNNNRTG 1 cut(s) 1328
SmlI CTYRAG 4 cut(s) 333, 434, 1055, 1934
SmoI CTYRAG 4 cut(s) 333, 434, 1055, 1934
SphI GCATGC 1 cut(s) 916
Sse9I AATT 9 cut(s) 279, 441, 510, 582, 1157, 1599, 2040, 2247, 2415
SspI AATATT 3 cut(s) 460, 478, 750
SspMI CTAG 8 cut(s) 572, 1844, 1862, 2003, 2060, 2088, 2259, 2265
StyD4I CCNGG 5 cut(s) 81, 82, 326, 1477, 2271
StyI CCWWGG 2 cut(s) 878, 2016
TaaI ACNGT 7 cut(s) 93, 544, 1426, 1464, 1768, 2127, 2172
TaiI ACGT 2 cut(s) 1178, 1300
TaqI TCGA 6 cut(s) 6, 106, 185, 334, 1945, 2231
TasI AATT 9 cut(s) 279, 441, 510, 582, 1157, 1599, 2040, 2247, 2415
TatI WGTACW 2 cut(s) 591, 1105
TauI GCSGC 4 cut(s) 31, 218, 418, 1625
TfiI GAWTC 6 cut(s) 178, 842, 2030, 2054, 2255, 2289
Tru1I TTAA 6 cut(s) 282, 1343, 1454, 1646, 1884, 2039
Tru9I TTAA 6 cut(s) 282, 1343, 1454, 1646, 1884, 2039
TscAI CASTG 5 cut(s) 96, 1330, 1469, 1771, 2299
TseFI GTSAC 5 cut(s) 143, 238, 289, 1370, 1817
TseI GCWGC 8 cut(s) 31, 195, 198, 257, 260, 263, 1060, 2212
Tsp45I GTSAC 5 cut(s) 143, 238, 289, 1370, 1817
TspDTI ATGAA 7 cut(s) 367, 813, 1156, 1287, 1575, 2067, 2328
TspGWI ACGGA 2 cut(s) 147, 226
TspMI CCCGGG 1 cut(s) 82
TspRI CASTG 5 cut(s) 96, 1330, 1469, 1771, 2299
Tth111I GACNNNGTC 1 cut(s) 149
VpaK11BI GGWCC 2 cut(s) 121, 324
VspI ATTAAT 1 cut(s) 2039
XagI CCTNNNNNAGG 1 cut(s) 1223
XapI RAATTY 3 cut(s) 441, 1157, 2247
XbaI TCTAGA 3 cut(s) 1843, 2002, 2087
XceI RCATGY 3 cut(s) 916, 1355, 2436
XhoI CTCGAG 1 cut(s) 333
XmaI CCCGGG 1 cut(s) 82
XspI CTAG 8 cut(s) 572, 1844, 1862, 2003, 2060, 2088, 2259, 2265
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.