Rw4G010750

Ion channel CASTOR-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001000
Physical Location & Seq
Reverse (-)
25151777 .. 25158685
6909 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G010750.1

Sequence Viewer

Length: 1482 bp
ATGTGTGTGTGTGTATATATATATATCCATTCTCGATGTCTAAGAAGTAATGATAGCAATATGGATTTTGTGGCCTATCCATGTGATTTCTTATACCATCGTTTGCAGGTATCTGTCTCCAAGGCACGTGCAATAATTGTCCTTGCTGAAGACGGAAATGCTGATCAGAGTGATGCCCGTGCATTGAGAACTGTTTTAAGTCTAACCGGAGTGAAAGAGGGGCTGAGGGGACATATTGTAGTTGAACTAAGTGATCTTGACAATGAGGTTCTTGTTAAACTTGTTGGTGGAGATCTTGTTGAAACTGTTGTGGCTCATGATGTAATTGGTCGTCTGATGATTCAATGTGCTAGGCAGCCCGGACTTGCACAGATCTGGGAAGATATCCTTGGATTTGAAAATTGCGAGTTCTATATCAAAAGATGGCCACAGTTGGATGGCATGCATTTTGAGGATGTACTGATCAGTTTTCCTGATGCCATTCCTTGTGGAGTCAAGGTTGCATCATTGGGTGGCAGAATTATTTTGAACCCGGATGACTCTTATGTTCTACAAGAAGGCGATGAAGTACTTGTCATAGCAGAGGATGATGATACCTATGCTCCTGCAGCATTGCCTATGGTCAAAGAGGCATCTTTCATTCAAATTTCCCGAACAGCAAGAAAGCCACAGAAGATTCTACTTTGTGGATGGAGGAGGGACATAGATGATATGCTTGTGGTGTTGGATGCCTTTCTGGCCCCGGGTTCCGAGCTCTGGATGTTTAATGACGTTGCTGAGAAGGAGAGGAAACAGAAGCTCATTGATGGAGGTCTTGAAATCAGTCGTTTAGTAAATATAACTTTAGTTGACCGTGAGGGAAATGCTGTCATACGCCGTCATTTAGAAAGTCTTCCTTTGCAATCTTTTGATTCGATCTTAATTTTGGCTGATGAGTCTGTGGAAGACTCAGCAATTCAAGCTGATTCAAGATCTCTTGCCACATTGCTTTTGATTCGGGATATTCAGGCTAAGCGTCTCCCTATGGTAACACATGTTAATAGAGGAAGCTTTTCACAAAGTTCATGGATAGGGGAAATGCAGCAAGCTTCAGATAAATCAGTTATAATTAGTGAAATTCTGGACCCAAGGACTAAAAATTTATTATCCGTGTCAAAGATCAGCGACTATGTGCTATCGAATGAGCTTGTCAGCATGGCACTGGCTATGGTAGCAGAGGATCGGCAAATAAATGATGTATTGGAAGAGCTGTTTGCAGAGGAGGGTAACGAATTGCAAATAAGGCAAGGAGATCTGTATCTCCATGAAGGCGAGGAGTTAAGCTTCTATGAAGTACTCTTGCGCGCCCGACAAAGGAGAGAGGTCATGATTGGTTATCGCTTAGCTGATACTGAGAGAGCTGTCATCAACCCCCCTGCTAAAAGTGAGAGACAGAGGTGGTCGGTGAAGGATGTATTTGTGGTGATTGCAGAGAAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Castor_Poll_mid

Protein Analysis

493

Amino Acids

55.47

Weight (kDa)

4.8

Isoelectric Point (pI)

47.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Castor_Poll_mid PF06241 106 - 203 4.2e-43 Castor and Pollux, part of voltage-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1106
Acc36I ACCTGC 1 cut(s) 97
AccII CGCG 1 cut(s) 1344
AclWI GGATC 1 cut(s) 1227
AcoI YGGCCR 1 cut(s) 425
AcsI RAATTY 3 cut(s) 645, 1116, 1138
AcuI CTGAAG 2 cut(s) 168, 1074
AcvI CACGTG 1 cut(s) 128
AfaI GTAC 3 cut(s) 459, 570, 1335
AfiI CCNNNNNNNGG 2 cut(s) 756, 1353
AflIII ACRYGT 1 cut(s) 1033
AgsI TTSAA 9 cut(s) 245, 302, 344, 398, 529, 644, 818, 959, 969
AjuI GAANNNNNNNTTGG 2 cut(s) 372, 404
Alw21I GWGCWC 1 cut(s) 756
Alw26I GTCTC 3 cut(s) 121, 1022, 1423
AlwI GGATC 1 cut(s) 1227
Ama87I CYCGRG 1 cut(s) 742
AoxI GGCC 3 cut(s) 72, 425, 738
ApeKI GCWGC 3 cut(s) 355, 608, 1081
ApoI RAATTY 3 cut(s) 645, 1116, 1138
Asp700I GAANNNNTTC 2 cut(s) 891, 1051
AspLEI GCGC 2 cut(s) 1344, 1346
AspS9I GGNCC 2 cut(s) 739, 1123
AsuC2I CCSGG 4 cut(s) 360, 533, 743, 744
AsuHPI GGTGA 2 cut(s) 1456, 1474
AvaI CYCGRG 1 cut(s) 742
AvaII GGWCC 1 cut(s) 1123
BalI TGGCCA 1 cut(s) 427
BanII GRGCYC 1 cut(s) 756
BbrPI CACGTG 1 cut(s) 128
BbsI GAAGAC 3 cut(s) 156, 884, 951
Bbv12I GWGCWC 1 cut(s) 756
BbvCI CCTCAGC 1 cut(s) 224
BbvI GCAGC 3 cut(s) 367, 620, 1093
BccI CCATC 5 cut(s) 105, 417, 431, 684, 800
BceAI ACGGC 1 cut(s) 861
BclI TGATCA 2 cut(s) 163, 462
BcnI CCSGG 4 cut(s) 360, 533, 743, 744
BcoDI GTCTC 3 cut(s) 121, 1022, 1423
BfaI CTAG 1 cut(s) 351
BfmI CTRYAG 1 cut(s) 606
BfuAI ACCTGC 1 cut(s) 97
BglI GCCNNNNNGGC 1 cut(s) 737
BglII AGATCT 4 cut(s) 292, 372, 971, 1291
BisI GCNGC 3 cut(s) 356, 609, 1082
BlpI GCTNAGC 2 cut(s) 1011, 1381
BlsI GCNGC 3 cut(s) 357, 610, 1083
BmcAI AGTACT 2 cut(s) 570, 1335
Bme1390I CCNGG 4 cut(s) 360, 533, 743, 744
Bme18I GGWCC 1 cut(s) 1123
BmeT110I CYCGRG 1 cut(s) 742
BmgT120I GGNCC 2 cut(s) 739, 1123
BmiI GGNNCC 3 cut(s) 741, 748, 1125
BmrFI CCNGG 4 cut(s) 360, 533, 743, 744
BmsI GCATC 5 cut(s) 163, 466, 512, 641, 718
BpiI GAAGAC 3 cut(s) 156, 884, 951
Bpu10I CCTNAGC 1 cut(s) 224
Bpu1102I GCTNAGC 2 cut(s) 1011, 1381
BpuMI CCSGG 4 cut(s) 360, 533, 743, 744
BsaAI YACGTR 1 cut(s) 128
BsaBI GATNNNNATC 1 cut(s) 1296
BsaJI CCNNGG 5 cut(s) 120, 388, 741, 742, 1127
BsaWI WCCGGW 1 cut(s) 206
BsaXI ACNNNNNCTCC 6 cut(s) 282, 312, 483, 513, 586, 616
Bsc4I CCNNNNNNNGG 2 cut(s) 756, 1353
Bse1I ACTGG 1 cut(s) 1206
Bse3DI GCAATG 2 cut(s) 611, 983
Bse8I GATNNNNATC 1 cut(s) 1296
BseDI CCNNGG 5 cut(s) 120, 388, 741, 742, 1127
BseGI GGATG 8 cut(s) 442, 460, 541, 592, 695, 733, 765, 1456
BseJI GATNNNNATC 1 cut(s) 1296
BseLI CCNNNNNNNGG 2 cut(s) 756, 1353
BseMI GCAATG 2 cut(s) 611, 983
BseMII CTCAG 4 cut(s) 215, 768, 963, 1383
BseNI ACTGG 1 cut(s) 1206
BsePI GCGCGC 1 cut(s) 1342
BseRI GAGGAG 3 cut(s) 709, 1274, 1328
BseXI GCAGC 3 cut(s) 367, 620, 1093
Bsh1236I CGCG 1 cut(s) 1344
BshFI GGCC 3 cut(s) 74, 427, 740
BsiHKAI GWGCWC 1 cut(s) 756
BsiHKCI CYCGRG 1 cut(s) 742
BsiSI CCGG 4 cut(s) 207, 360, 533, 743
BslFI GGGAC 2 cut(s) 243, 713
BslI CCNNNNNNNGG 2 cut(s) 756, 1353
BsmAI GTCTC 3 cut(s) 121, 1022, 1423
BsmBI CGTCTC 1 cut(s) 1022
BsmFI GGGAC 2 cut(s) 243, 713
BsnI GGCC 3 cut(s) 74, 427, 740
BsoBI CYCGRG 1 cut(s) 742
Bsp1286I GDGCHC 1 cut(s) 756
Bsp1720I GCTNAGC 2 cut(s) 1011, 1381
BspANI GGCC 3 cut(s) 74, 427, 740
BspCNI CTCAG 4 cut(s) 216, 769, 962, 1384
BspFNI CGCG 1 cut(s) 1344
BspHI TCATGA 2 cut(s) 316, 1365
BspLI GGNNCC 3 cut(s) 741, 748, 1125
BspMAI CTGCAG 1 cut(s) 610
BspMI ACCTGC 1 cut(s) 97
BspPI GGATC 1 cut(s) 1227
BspQI GCTCTTC 1 cut(s) 1239
BsrDI GCAATG 2 cut(s) 611, 983
BsrI ACTGG 1 cut(s) 1206
BssECI CCNNGG 5 cut(s) 120, 388, 741, 742, 1127
BssHII GCGCGC 1 cut(s) 1342
BssT1I CCWWGG 3 cut(s) 120, 388, 1127
Bst4CI ACNGT 4 cut(s) 193, 307, 432, 854
Bst6I CTCTTC 1 cut(s) 1239
BstBAI YACGTR 1 cut(s) 128
BstC8I GCNNGC 3 cut(s) 443, 1086, 1344
BstDEI CTNAG 8 cut(s) 41, 224, 248, 777, 949, 1011, 1381, 1392
BstF5I GGATG 8 cut(s) 442, 460, 541, 592, 695, 733, 765, 1456
BstFNI CGCG 1 cut(s) 1344
BstHHI GCGC 2 cut(s) 1344, 1346
BstMAI GTCTC 3 cut(s) 121, 1022, 1423
BstMWI GCNNNNNNNGC 5 cut(s) 608, 737, 959, 1211, 1282
BstNSI RCATGY 2 cut(s) 445, 1037
BstSCI CCNGG 4 cut(s) 358, 531, 741, 742
BstSFI CTRYAG 1 cut(s) 606
BstUI CGCG 1 cut(s) 1344
BstV1I GCAGC 3 cut(s) 367, 620, 1093
BstV2I GAAGAC 3 cut(s) 156, 884, 951
BstX2I RGATCY 4 cut(s) 292, 372, 971, 1291
BstYI RGATCY 4 cut(s) 292, 372, 971, 1291
BsuRI GGCC 3 cut(s) 74, 427, 740
BtgZI GCGATG 1 cut(s) 576
BtsCI GGATG 8 cut(s) 442, 460, 541, 592, 695, 733, 765, 1456
BtsIMutI CAGTG 1 cut(s) 1199
BveI ACCTGC 1 cut(s) 97
Cac8I GCNNGC 3 cut(s) 443, 1086, 1344
CciI TCATGA 2 cut(s) 316, 1365
CfoI GCGC 2 cut(s) 1344, 1346
Cfr13I GGNCC 2 cut(s) 739, 1123
Cfr9I CCCGGG 1 cut(s) 742
CseI GACGC 1 cut(s) 1004
Csp6I GTAC 3 cut(s) 458, 569, 1334
CviAII CATG 8 cut(s) 81, 317, 442, 1034, 1065, 1195, 1304, 1366
CviQI GTAC 3 cut(s) 458, 569, 1334
DdeI CTNAG 8 cut(s) 41, 224, 248, 777, 949, 1011, 1381, 1392
EaeI YGGCCR 1 cut(s) 425
Eam1104I CTCTTC 1 cut(s) 1239
EarI CTCTTC 1 cut(s) 1239
Ecl136II GAGCTC 1 cut(s) 754
Eco130I CCWWGG 3 cut(s) 120, 388, 1127
Eco24I GRGCYC 1 cut(s) 756
Eco32I GATATC 1 cut(s) 385
Eco47I GGWCC 1 cut(s) 1123
Eco53kI GAGCTC 1 cut(s) 754
Eco57I CTGAAG 2 cut(s) 168, 1074
Eco72I CACGTG 1 cut(s) 128
Eco88I CYCGRG 1 cut(s) 742
EcoICRI GAGCTC 1 cut(s) 754
EcoRV GATATC 1 cut(s) 385
EcoT14I CCWWGG 3 cut(s) 120, 388, 1127
EcoT22I ATGCAT 1 cut(s) 447
EcoT38I GRGCYC 1 cut(s) 756
ErhI CCWWGG 3 cut(s) 120, 388, 1127
Esp3I CGTCTC 1 cut(s) 1022
FaeI CATG 8 cut(s) 84, 320, 445, 1037, 1068, 1198, 1307, 1369
FalI AAGNNNNNCTT 4 cut(s) 372, 404, 880, 912
FaqI GGGAC 2 cut(s) 243, 713
FatI CATG 8 cut(s) 80, 316, 441, 1033, 1064, 1194, 1303, 1365
FbaI TGATCA 2 cut(s) 163, 462
Fnu4HI GCNGC 3 cut(s) 356, 609, 1082
FokI GGATG 8 cut(s) 449, 467, 548, 599, 702, 740, 772, 1463
FriOI GRGCYC 1 cut(s) 756
Fsp4HI GCNGC 3 cut(s) 356, 609, 1082
FspBI CTAG 1 cut(s) 351
GlaI GCGC 2 cut(s) 1343, 1345
GluI GCNGC 3 cut(s) 356, 609, 1082
HaeIII GGCC 3 cut(s) 74, 427, 740
HapII CCGG 4 cut(s) 207, 360, 533, 743
HgaI GACGC 1 cut(s) 1004
HhaI GCGC 2 cut(s) 1344, 1346
Hin1II CATG 8 cut(s) 84, 320, 445, 1037, 1068, 1198, 1307, 1369
Hin6I GCGC 2 cut(s) 1342, 1344
HinP1I GCGC 2 cut(s) 1342, 1344
HincII GTYRAC 1 cut(s) 850
HindII GTYRAC 1 cut(s) 850
HindIII AAGCTT 3 cut(s) 1048, 1086, 1321
HinfI GANTC 9 cut(s) 340, 492, 539, 676, 911, 935, 947, 965, 994
HpaII CCGG 4 cut(s) 207, 360, 533, 743
HphI GGTGA 2 cut(s) 1456, 1474
Hpy166II GTNNAC 1 cut(s) 850
Hpy188I TCNGA 4 cut(s) 168, 336, 751, 1093
Hpy8I GTNNAC 1 cut(s) 850
HpyAV CCTTC 5 cut(s) 551, 775, 1301, 1441, 1468
HpyCH4III ACNGT 4 cut(s) 193, 307, 432, 854
HpyCH4IV ACGT 2 cut(s) 127, 771
HpyF10VI GCNNNNNNNGC 5 cut(s) 608, 737, 959, 1211, 1282
HpyF3I CTNAG 8 cut(s) 41, 224, 248, 777, 949, 1011, 1381, 1392
HpySE526I ACGT 2 cut(s) 127, 771
Hsp92II CATG 8 cut(s) 84, 320, 445, 1037, 1068, 1198, 1307, 1369
HspAI GCGC 2 cut(s) 1342, 1344
Ksp22I TGATCA 2 cut(s) 163, 462
LguI GCTCTTC 1 cut(s) 1239
LmnI GCTCC 1 cut(s) 607
Lsp1109I GCAGC 3 cut(s) 367, 620, 1093
LweI GCATC 5 cut(s) 163, 466, 512, 641, 718
MaeI CTAG 1 cut(s) 351
MaeII ACGT 2 cut(s) 127, 771
MaeIII GTNAC 2 cut(s) 1027, 1265
MboII GAAGA 6 cut(s) 161, 392, 685, 884, 956, 1256
MflI RGATCY 4 cut(s) 292, 372, 971, 1291
MhlI GDGCHC 1 cut(s) 756
MlsI TGGCCA 1 cut(s) 427
MluNI TGGCCA 1 cut(s) 427
MlyI GAGTC 4 cut(s) 501, 533, 941, 944
MmeI TCCRAC 2 cut(s) 414, 705
Mox20I TGGCCA 1 cut(s) 427
Mph1103I ATGCAT 1 cut(s) 447
MroXI GAANNNNTTC 2 cut(s) 891, 1051
MscI TGGCCA 1 cut(s) 427
MseI TTAA 6 cut(s) 197, 276, 765, 920, 1038, 1319
Msp20I TGGCCA 1 cut(s) 427
MspI CCGG 4 cut(s) 207, 360, 533, 743
MspR9I CCNGG 4 cut(s) 360, 533, 743, 744
MvnI CGCG 1 cut(s) 1344
MwoI GCNNNNNNNGC 5 cut(s) 608, 737, 959, 1211, 1282
NciI CCSGG 4 cut(s) 360, 533, 743, 744
NlaIII CATG 8 cut(s) 84, 320, 445, 1037, 1068, 1198, 1307, 1369
NlaIV GGNNCC 3 cut(s) 741, 748, 1125
NsiI ATGCAT 1 cut(s) 447
NspI RCATGY 2 cut(s) 445, 1037
PaeI GCATGC 1 cut(s) 445
PagI TCATGA 2 cut(s) 316, 1365
PauI GCGCGC 1 cut(s) 1342
PciI ACATGT 1 cut(s) 1033
PciSI GCTCTTC 1 cut(s) 1239
PdmI GAANNNNTTC 2 cut(s) 891, 1051
PfeI GAWTC 5 cut(s) 340, 676, 911, 965, 994
PkrI GCNGC 3 cut(s) 357, 610, 1083
PleI GAGTC 4 cut(s) 500, 533, 941, 943
PmaCI CACGTG 1 cut(s) 128
PmlI CACGTG 1 cut(s) 128
PpsI GAGTC 4 cut(s) 500, 533, 941, 943
Ppu21I YACGTR 1 cut(s) 128
PscI ACATGT 1 cut(s) 1033
PsiI TTATAA 1 cut(s) 1106
Psp124BI GAGCTC 1 cut(s) 756
PspCI CACGTG 1 cut(s) 128
PspN4I GGNNCC 3 cut(s) 741, 748, 1125
PspPI GGNCC 2 cut(s) 739, 1123
PstI CTGCAG 1 cut(s) 610
PsuI RGATCY 4 cut(s) 292, 372, 971, 1291
PteI GCGCGC 1 cut(s) 1342
RsaI GTAC 3 cut(s) 459, 570, 1335
RsaNI GTAC 3 cut(s) 458, 569, 1334
SacI GAGCTC 1 cut(s) 756
SapI GCTCTTC 1 cut(s) 1239
SaqAI TTAA 6 cut(s) 197, 276, 765, 920, 1038, 1319
SatI GCNGC 3 cut(s) 356, 609, 1082
Sau96I GGNCC 2 cut(s) 739, 1123
ScaI AGTACT 2 cut(s) 570, 1335
SchI GAGTC 4 cut(s) 501, 533, 941, 944
ScrFI CCNGG 4 cut(s) 360, 533, 743, 744
SduI GDGCHC 1 cut(s) 756
SfaNI GCATC 5 cut(s) 163, 466, 512, 641, 718
SfcI CTRYAG 1 cut(s) 606
SinI GGWCC 1 cut(s) 1123
SmaI CCCGGG 1 cut(s) 744
SphI GCATGC 1 cut(s) 445
SspMI CTAG 1 cut(s) 351
SstI GAGCTC 1 cut(s) 756
StyD4I CCNGG 4 cut(s) 358, 531, 741, 742
StyI CCWWGG 3 cut(s) 120, 388, 1127
TaaI ACNGT 4 cut(s) 193, 307, 432, 854
TaiI ACGT 2 cut(s) 130, 774
TaqI TCGA 3 cut(s) 34, 914, 1178
TatI WGTACW 3 cut(s) 457, 568, 1333
TfiI GAWTC 5 cut(s) 340, 676, 911, 965, 994
Tru1I TTAA 6 cut(s) 197, 276, 765, 920, 1038, 1319
Tru9I TTAA 6 cut(s) 197, 276, 765, 920, 1038, 1319
TscAI CASTG 1 cut(s) 1206
TseI GCWGC 3 cut(s) 355, 608, 1081
TspDTI ATGAA 5 cut(s) 579, 628, 1053, 1320, 1344
TspGWI ACGGA 2 cut(s) 168, 1138
TspMI CCCGGG 1 cut(s) 742
TspRI CASTG 1 cut(s) 1206
VpaK11BI GGWCC 1 cut(s) 1123
XapI RAATTY 3 cut(s) 645, 1116, 1138
XceI RCATGY 2 cut(s) 445, 1037
XmaI CCCGGG 1 cut(s) 742
XmnI GAANNNNTTC 2 cut(s) 891, 1051
XspI CTAG 1 cut(s) 351
ZrmI AGTACT 2 cut(s) 570, 1335
Zsp2I ATGCAT 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.