RLG00000008832

Ion channel CASTOR-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0001000
Physical Location & Seq
Forward (+)
40489185 .. 40498101
8917 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008832

Sequence Viewer

Length: 2667 bp
ATGTCCCTCGACTCCGACTCAGCAGTCCCTTCCTCCAGCAGAGACTGGTACTTTCCCTCACCGCCCTTCATCCACGCAGCGAAATCCCCAAAATACCACCGAAGATTCTCCACAAACCCCAGACCATCCCCACCCCGCCCCACCGATTCTCAGCCGCCATTTGGTCCGGCGACGCCGTTTCGCGCCGTTTCGTCCTCCAGCTCGGCCCCTCCGAGAACTTTCAGCCATGACGGGCCCCGTCGGAGAGTTGACTTCCCCCGTCGGCGGGAGAAGCCGCCGCGAAACGATGTCAACGAGCCCGTTTCCGGCGTCCCCCACGGCCTCTCCAAAAAACGATCCGAGGCTTCCGCTGCCGTCTCCGGCGAAAAGTTCATTAGCTTTTCCGGCCTCGGCTTCAAACCCCGGTGGAAAATGGCTTTTTCAGTAGCGATTTTGATGACGGTCTTGTCATCAGTGGTGTACCAAAATTTCTGTTTACATGGCCAAGTCAATGAGTTGCAGGATTATATTTCCAAGCTTGAATTGAGATTACAAGCCTACAATTCGTTAGAATCTATAGAGCTCATCACTTATTCTGGGCTGGAGGATGATGATCTTCCTAACAGAAGCTTGAAACGATTAGCGCTTACGGTCTCCCTCATGTTTTTATCAATCCCTGTTGTGGTTTTCAAGTACATTGACTATGTCTCGAGATCACGGTCACCGGAGAACATCTCTGAAGAAGAATCTCTAAACAAACAGCTTGCGTACCGTGTCGATTTGTTTTTATCAGTCCACCCGTATTCTAAGCCACTGACATTGTTGGTTGCAACACTGTTACTTATTTGTCTTGGGGGGTTAGCATTGTTTGGAGTGAAAGATGATAGCTTAGCGGAATGTCTTTGGTTATCGTGGACGTATGTAGCTGATTCTGGCAACCATACTGACTCTGAAAAAATTGGCGAAAGGCTTGTTTCAGTTTCCATTAGCTTTGGTGGGATGCTCATATTTGCAATGATGCTTGGACTTGTTTCTGATGCAATTTCGGAGAAGTTTGACTCATTGAGGAAAGGAAGAAGTGAGGTTGTGGAGCAAAACCATACTTTAATTCTCGGTTGGAGCGATAAATTGGGATCATTATTGAACCAGATTGCAATAGCTAATGAGAGTTTGGGTGGAGGGATTGTTGTAGTTATGGCTGAGCGAGACAAAGAGGAAATGGAACTTGACATTGGTAAAATGGAGTTTGATTTCAAAGGAACATCTGTAATATGCAGAAGTGGGAGCCCTCTTATTCTTGCTGACCTGAAAAAGGTATCTGTCTCCAAGGCACGTGCAATAATTGTCCTTGCTGAAGACGGAAATGCTGATCAGAGTGATGCCCGTGCATTGAGAACTGTTTTAAGTCTAACCGGAGTGAAAGAGGGGCTGAGGGGACATATTGTGGTTGAACTAAGTGATCTTGACAATGAGGTTCTTGTTAAACTTGTTGGTGGAGATCTTGTTGAAACTGTTGTGGCTCATGATGTAATTGGTCGTCTGATGATTCAATGTGCTAGGCAGCCCGGACTTGCACAGATCTGGGAAGATATCCTTGGATTTGAAAATTGCGAGTTCTATATCAAAAGATGGCCACAGTTGGATGGCATGCATTTTGAGGATGTACTGATCAGTTTTCCTGATGCCATTCCTTGTGGAGTCAAGGTTGCATCATTGGGTGGTAGAATTATTTTGAACCCGGATGACTCTTATGTTCTACAAGAAGGCGATGAAGTACTTGTCATAGCAGAGGATGACGATACCTATGCTCCTGCAGCATTGCCTATGGTATGGAGAGGAAGTCTACCCAAAGACTTTGTTGTTCCAAAGTCTGCAGAAAGGATACTTTTATGTGGTTGGCGGCGAGATATGGAGGATATGATTATGGTGTTGGATGCCTTTCTGGCCCCGGGTTCCGAGCTCTGGATGTTCAATGATGTTGCTGAGAAGGAGAGGAAACAGAAGCTCATTGATGGAGGTCTTGAAATCAGTCGTTTAGTAAATATATCTTTAGTTGACCGTGAGGGAAATGCTGTCATACGCCGTCATTTAGAAAGTCTTCCTTTGCAATCTTTTGATTCGATCTTAATTTTGGCCGATGAGTCTGTGGAAGACTCAGCAATTCAAGCTGATTCAAGATCTCTTGCCACATTGCTTTTGATTCGTGATATTCAGGCTAAGCGTCTCCCTATGGTAACACATGTTGATAGAGGAAGCTTTTCACAAAGTTCATGGATAGGGGAAATGCAGCAAGCTTCAGATAAGTCAGTTATAATTAGTGAAATTCTGGACCCAAGGACTAAAAATTTATTATCCATGTCAAAGATCAGCGACTATGTGCTATCGAATGAGCTTGTCAGCATGGCACTGGCTATGGTAGCAGAGGATCGGCAAATAAATGATGTATTGGAAGAGCTGTTTGCAGAGGAGGGTAACGAATTGCAAATAAGGCAAGGAGATCTGTATCTCCGTGAAGGTGAGGAGCTAAGCTTCTATGAAGTACTCTTGCGTGCCCGACAAAGGAGAGAGGTCATGATTGGTTATCGCTTAGCTGATGCTGAGAGAGCTGTCATCAACCCCCCTGCTAAAAGTGAGAGACAGAGGTGGTCGGTGAAGGATGTATTTGTGGTGATTGCAGAGAAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Castor_Poll_mid

Protein Analysis

889

Amino Acids

98.95

Weight (kDa)

5.15

Isoelectric Point (pI)

55.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Castor_Poll_mid PF06241 501 - 598 1.2e-42 Castor and Pollux, part of voltage-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2291
AasI GACNNNNNNGTC 2 cut(s) 23, 445
AccI GTMKAC 1 cut(s) 1822
AccII CGCG 2 cut(s) 183, 280
AciI CCGC 9 cut(s) 62, 136, 155, 265, 275, 278, 348, 872, 1879
AclWI GGATC 3 cut(s) 330, 1120, 2412
AcoI YGGCCR 3 cut(s) 481, 1610, 2112
AcsI RAATTY 3 cut(s) 466, 2301, 2323
AcuI CTGAAG 3 cut(s) 738, 1353, 2259
AcvI CACGTG 1 cut(s) 1313
AcyI GRCGYC 2 cut(s) 173, 309
AfaI GTAC 7 cut(s) 50, 461, 674, 749, 1644, 1755, 2520
AfeI AGCGCT 1 cut(s) 624
AfiI CCNNNNNNNGG 8 cut(s) 161, 264, 265, 305, 661, 1291, 1941, 2538
AflIII ACRYGT 1 cut(s) 2218
AjuI GAANNNNNNNTTGG 4 cut(s) 1194, 1226, 1557, 1589
Alw21I GWGCWC 2 cut(s) 564, 1941
Alw26I GTCTC 8 cut(s) 36, 361, 637, 691, 1179, 1306, 2207, 2608
AlwI GGATC 3 cut(s) 330, 1120, 2412
AlwNI CAGNNNCTG 1 cut(s) 45
Ama87I CYCGRG 2 cut(s) 688, 1927
Aor51HI AGCGCT 1 cut(s) 624
AoxI GGCC 8 cut(s) 204, 233, 319, 385, 481, 1610, 1923, 2112
ApaI GGGCCC 1 cut(s) 237
ApeKI GCWGC 5 cut(s) 77, 350, 1540, 1793, 2266
ApoI RAATTY 3 cut(s) 466, 2301, 2323
Asp700I GAANNNNTTC 2 cut(s) 2076, 2236
AspLEI GCGC 2 cut(s) 185, 625
AspS9I GGNCC 6 cut(s) 164, 205, 233, 234, 1924, 2308
AsuC2I CCSGG 5 cut(s) 403, 1545, 1718, 1928, 1929
AsuHPI GGTGA 5 cut(s) 51, 693, 2507, 2641, 2659
AvaI CYCGRG 2 cut(s) 688, 1927
AvaII GGWCC 2 cut(s) 164, 2308
BaeGI GKGCMC 2 cut(s) 237, 2533
BalI TGGCCA 2 cut(s) 483, 1612
BanII GRGCYC 5 cut(s) 237, 300, 564, 1268, 1941
BbrPI CACGTG 1 cut(s) 1313
BbsI GAAGAC 3 cut(s) 1341, 2069, 2136
Bbv12I GWGCWC 2 cut(s) 564, 1941
BbvCI CCTCAGC 1 cut(s) 1409
BbvI GCAGC 5 cut(s) 89, 337, 1552, 1805, 2278
BccI CCATC 4 cut(s) 133, 1602, 1616, 1985
BceAI ACGGC 5 cut(s) 160, 170, 334, 338, 2046
BcgI CGANNNNNNTGC 2 cut(s) 1766, 1800
BciVI GTATCC 1 cut(s) 1854
BclI TGATCA 2 cut(s) 1348, 1647
BcnI CCSGG 5 cut(s) 403, 1545, 1718, 1928, 1929
BcoDI GTCTC 8 cut(s) 36, 361, 637, 691, 1179, 1306, 2207, 2608
BfaI CTAG 1 cut(s) 1536
BfmI CTRYAG 3 cut(s) 555, 1791, 1851
BfoI RGCGCY 1 cut(s) 626
BfuI GTATCC 1 cut(s) 1854
BglI GCCNNNNNGGC 1 cut(s) 1922
BglII AGATCT 4 cut(s) 1477, 1557, 2156, 2476
BisI GCNGC 9 cut(s) 78, 155, 275, 278, 351, 1541, 1794, 1880, 2267
BlpI GCTNAGC 5 cut(s) 868, 1179, 2196, 2504, 2566
BlsI GCNGC 9 cut(s) 79, 156, 276, 279, 352, 1542, 1795, 1881, 2268
BmcAI AGTACT 2 cut(s) 1755, 2520
Bme1390I CCNGG 5 cut(s) 403, 1545, 1718, 1928, 1929
Bme18I GGWCC 2 cut(s) 164, 2308
BmeT110I CYCGRG 2 cut(s) 688, 1927
BmgT120I GGNCC 6 cut(s) 164, 205, 233, 234, 1924, 2308
BmiI GGNNCC 7 cut(s) 207, 235, 236, 1265, 1926, 1933, 2310
BmrFI CCNGG 5 cut(s) 403, 1545, 1718, 1928, 1929
BmsI GCATC 8 cut(s) 969, 987, 1006, 1348, 1651, 1697, 1903, 2563
BpiI GAAGAC 3 cut(s) 1341, 2069, 2136
BplI GAGNNNNNCTC 2 cut(s) 698, 730
BpmI CTGGAG 3 cut(s) 19, 181, 602
Bpu10I CCTNAGC 1 cut(s) 1409
Bpu1102I GCTNAGC 5 cut(s) 868, 1179, 2196, 2504, 2566
BpuMI CCSGG 5 cut(s) 403, 1545, 1718, 1928, 1929
BsaAI YACGTR 1 cut(s) 1313
BsaBI GATNNNNATC 2 cut(s) 591, 2481
BsaHI GRCGYC 2 cut(s) 173, 309
BsaI GGTCTC 1 cut(s) 637
BsaJI CCNNGG 9 cut(s) 316, 339, 388, 401, 1305, 1573, 1926, 1927, 2312
BsaWI WCCGGW 2 cut(s) 703, 1391
Bsc4I CCNNNNNNNGG 8 cut(s) 161, 264, 265, 305, 661, 1291, 1941, 2538
Bse1I ACTGG 2 cut(s) 50, 2391
Bse3DI GCAATG 3 cut(s) 999, 1796, 2168
Bse8I GATNNNNATC 2 cut(s) 591, 2481
BseDI CCNNGG 9 cut(s) 316, 339, 388, 401, 1305, 1573, 1926, 1927, 2312
BseJI GATNNNNATC 2 cut(s) 591, 2481
BseLI CCNNNNNNNGG 8 cut(s) 161, 264, 265, 305, 661, 1291, 1941, 2538
BseMI GCAATG 3 cut(s) 999, 1796, 2168
BseMII CTCAG 7 cut(s) 33, 164, 1170, 1400, 1953, 2148, 2568
BseNI ACTGG 2 cut(s) 50, 2391
BseRI GAGGAG 2 cut(s) 2459, 2513
BseSI GKGCMC 2 cut(s) 237, 2533
BseXI GCAGC 5 cut(s) 89, 337, 1552, 1805, 2278
Bsh1236I CGCG 2 cut(s) 183, 280
BshFI GGCC 8 cut(s) 206, 235, 321, 387, 483, 1612, 1925, 2114
BsiHKAI GWGCWC 2 cut(s) 564, 1941
BsiHKCI CYCGRG 2 cut(s) 688, 1927
BslFI GGGAC 3 cut(s) 11, 296, 1428
BslI CCNNNNNNNGG 8 cut(s) 161, 264, 265, 305, 661, 1291, 1941, 2538
BsmAI GTCTC 8 cut(s) 36, 361, 637, 691, 1179, 1306, 2207, 2608
BsmBI CGTCTC 2 cut(s) 361, 2207
BsmFI GGGAC 3 cut(s) 11, 296, 1428
BsnI GGCC 8 cut(s) 206, 235, 321, 387, 483, 1612, 1925, 2114
Bso31I GGTCTC 1 cut(s) 637
BsoBI CYCGRG 2 cut(s) 688, 1927
Bsp120I GGGCCC 1 cut(s) 233
Bsp1286I GDGCHC 6 cut(s) 237, 300, 564, 1268, 1941, 2533
Bsp1720I GCTNAGC 5 cut(s) 868, 1179, 2196, 2504, 2566
BspACI CCGC 9 cut(s) 62, 136, 155, 265, 275, 278, 348, 872, 1879
BspANI GGCC 8 cut(s) 206, 235, 321, 387, 483, 1612, 1925, 2114
BspCNI CTCAG 7 cut(s) 32, 163, 1171, 1401, 1954, 2147, 2569
BspFNI CGCG 2 cut(s) 183, 280
BspHI TCATGA 2 cut(s) 1501, 2550
BspLI GGNNCC 7 cut(s) 207, 235, 236, 1265, 1926, 1933, 2310
BspMAI CTGCAG 2 cut(s) 1795, 1855
BspPI GGATC 3 cut(s) 330, 1120, 2412
BspQI GCTCTTC 1 cut(s) 2424
BspTNI GGTCTC 1 cut(s) 637
BsrDI GCAATG 3 cut(s) 999, 1796, 2168
BsrI ACTGG 2 cut(s) 50, 2391
BssECI CCNNGG 9 cut(s) 316, 339, 388, 401, 1305, 1573, 1926, 1927, 2312
BssNI GRCGYC 2 cut(s) 173, 309
BssT1I CCWWGG 3 cut(s) 1305, 1573, 2312
Bst4CI ACNGT 9 cut(s) 442, 631, 699, 752, 816, 1378, 1492, 1617, 2039
Bst6I CTCTTC 1 cut(s) 2424
BstACI GRCGYC 2 cut(s) 173, 309
BstBAI YACGTR 1 cut(s) 1313
BstC8I GCNNGC 4 cut(s) 744, 1628, 2271, 2529
BstDSI CCRYGG 1 cut(s) 316
BstEII GGTNACC 1 cut(s) 699
BstENI CCTNNNNNAGG 1 cut(s) 1289
BstFNI CGCG 2 cut(s) 183, 280
BstH2I RGCGCY 1 cut(s) 626
BstHHI GCGC 2 cut(s) 185, 625
BstMAI GTCTC 8 cut(s) 36, 361, 637, 691, 1179, 1306, 2207, 2608
BstMWI GCNNNNNNNGC 9 cut(s) 271, 350, 384, 1793, 1922, 2144, 2396, 2467, 2582
BstNSI RCATGY 2 cut(s) 1630, 2222
BstPI GGTNACC 1 cut(s) 699
BstSCI CCNGG 5 cut(s) 401, 1543, 1716, 1926, 1927
BstSFI CTRYAG 3 cut(s) 555, 1791, 1851
BstSLI GKGCMC 2 cut(s) 237, 2533
BstUI CGCG 2 cut(s) 183, 280
BstV1I GCAGC 5 cut(s) 89, 337, 1552, 1805, 2278
BstV2I GAAGAC 3 cut(s) 1341, 2069, 2136
BstX2I RGATCY 4 cut(s) 1477, 1557, 2156, 2476
BstYI RGATCY 4 cut(s) 1477, 1557, 2156, 2476
BsuI GTATCC 1 cut(s) 1854
BsuRI GGCC 8 cut(s) 206, 235, 321, 387, 483, 1612, 1925, 2114
BtgI CCRYGG 1 cut(s) 316
BtgZI GCGATG 1 cut(s) 1761
BtsIMutI CAGTG 4 cut(s) 459, 791, 812, 2384
Cac8I GCNNGC 4 cut(s) 744, 1628, 2271, 2529
CaiI CAGNNNCTG 1 cut(s) 45
CciI TCATGA 2 cut(s) 1501, 2550
CfoI GCGC 2 cut(s) 185, 625
Cfr13I GGNCC 6 cut(s) 164, 205, 233, 234, 1924, 2308
Cfr9I CCCGGG 1 cut(s) 1927
CseI GACGC 3 cut(s) 181, 298, 2189
Csp6I GTAC 7 cut(s) 49, 460, 673, 748, 1643, 1754, 2519
CspCI CAANNNNNGTGG 2 cut(s) 780, 815
CviQI GTAC 7 cut(s) 49, 460, 673, 748, 1643, 1754, 2519
DrdI GACNNNNNNGTC 2 cut(s) 23, 445
DseDI GACNNNNNNGTC 2 cut(s) 23, 445
EaeI YGGCCR 3 cut(s) 481, 1610, 2112
Eam1104I CTCTTC 1 cut(s) 2424
EarI CTCTTC 1 cut(s) 2424
Ecl136II GAGCTC 2 cut(s) 562, 1939
Eco130I CCWWGG 3 cut(s) 1305, 1573, 2312
Eco24I GRGCYC 5 cut(s) 237, 300, 564, 1268, 1941
Eco31I GGTCTC 1 cut(s) 637
Eco32I GATATC 1 cut(s) 1570
Eco47I GGWCC 2 cut(s) 164, 2308
Eco47III AGCGCT 1 cut(s) 624
Eco53kI GAGCTC 2 cut(s) 562, 1939
Eco57I CTGAAG 3 cut(s) 738, 1353, 2259
Eco72I CACGTG 1 cut(s) 1313
Eco88I CYCGRG 2 cut(s) 688, 1927
Eco91I GGTNACC 1 cut(s) 699
EcoICRI GAGCTC 2 cut(s) 562, 1939
EcoNI CCTNNNNNAGG 1 cut(s) 1289
EcoO109I RGGNCCY 1 cut(s) 234
EcoO65I GGTNACC 1 cut(s) 699
EcoRV GATATC 1 cut(s) 1570
EcoT14I CCWWGG 3 cut(s) 1305, 1573, 2312
EcoT22I ATGCAT 1 cut(s) 1632
EcoT38I GRGCYC 5 cut(s) 237, 300, 564, 1268, 1941
ErhI CCWWGG 3 cut(s) 1305, 1573, 2312
Esp3I CGTCTC 2 cut(s) 361, 2207
FalI AAGNNNNNCTT 4 cut(s) 1557, 1589, 2065, 2097
FaqI GGGAC 3 cut(s) 11, 296, 1428
FauI CCCGC 2 cut(s) 143, 258
FbaI TGATCA 2 cut(s) 1348, 1647
FblI GTMKAC 1 cut(s) 1822
Fnu4HI GCNGC 9 cut(s) 78, 155, 275, 278, 351, 1541, 1794, 1880, 2267
FriOI GRGCYC 5 cut(s) 237, 300, 564, 1268, 1941
Fsp4HI GCNGC 9 cut(s) 78, 155, 275, 278, 351, 1541, 1794, 1880, 2267
FspBI CTAG 1 cut(s) 1536
GlaI GCGC 2 cut(s) 184, 624
GluI GCNGC 9 cut(s) 78, 155, 275, 278, 351, 1541, 1794, 1880, 2267
GsuI CTGGAG 3 cut(s) 19, 181, 602
HaeII RGCGCY 1 cut(s) 626
HaeIII GGCC 8 cut(s) 206, 235, 321, 387, 483, 1612, 1925, 2114
HgaI GACGC 3 cut(s) 181, 298, 2189
HhaI GCGC 2 cut(s) 185, 625
Hin1I GRCGYC 2 cut(s) 173, 309
Hin6I GCGC 2 cut(s) 183, 623
HinP1I GCGC 2 cut(s) 183, 623
HincII GTYRAC 3 cut(s) 250, 292, 2035
HindII GTYRAC 3 cut(s) 250, 292, 2035
HindIII AAGCTT 5 cut(s) 515, 607, 2233, 2271, 2506
HphI GGTGA 5 cut(s) 51, 693, 2507, 2641, 2659
Hpy166II GTNNAC 8 cut(s) 250, 292, 460, 476, 775, 894, 1823, 2035
Hpy8I GTNNAC 8 cut(s) 250, 292, 460, 476, 775, 894, 1823, 2035
Hpy99I CGWCG 3 cut(s) 175, 243, 264
HpyAV CCTTC 7 cut(s) 39, 76, 1736, 1960, 2486, 2626, 2653
HpyCH4III ACNGT 9 cut(s) 442, 631, 699, 752, 816, 1378, 1492, 1617, 2039
HpyCH4IV ACGT 2 cut(s) 896, 1312
HpyF10VI GCNNNNNNNGC 9 cut(s) 271, 350, 384, 1793, 1922, 2144, 2396, 2467, 2582
HpySE526I ACGT 2 cut(s) 896, 1312
Hsp92I GRCGYC 2 cut(s) 173, 309
HspAI GCGC 2 cut(s) 183, 623
Ksp22I TGATCA 2 cut(s) 1348, 1647
LguI GCTCTTC 1 cut(s) 2424
LmnI GCTCC 5 cut(s) 1069, 1098, 1263, 1792, 2500
Lsp1109I GCAGC 5 cut(s) 89, 337, 1552, 1805, 2278
LweI GCATC 8 cut(s) 969, 987, 1006, 1348, 1651, 1697, 1903, 2563
MaeI CTAG 1 cut(s) 1536
MaeII ACGT 2 cut(s) 896, 1312
MaeIII GTNAC 4 cut(s) 699, 816, 2212, 2450
MflI RGATCY 4 cut(s) 1477, 1557, 2156, 2476
MhlI GDGCHC 6 cut(s) 237, 300, 564, 1268, 1941, 2533
MlsI TGGCCA 2 cut(s) 483, 1612
MluNI TGGCCA 2 cut(s) 483, 1612
MlyI GAGTC 8 cut(s) 5, 11, 920, 1031, 1686, 1718, 2126, 2129
MmeI TCCRAC 5 cut(s) 39, 221, 1076, 1599, 1890
Mox20I TGGCCA 2 cut(s) 483, 1612
Mph1103I ATGCAT 1 cut(s) 1632
MroXI GAANNNNTTC 2 cut(s) 2076, 2236
MscI TGGCCA 2 cut(s) 483, 1612
MseI TTAA 4 cut(s) 1085, 1382, 1461, 2105
Msp20I TGGCCA 2 cut(s) 483, 1612
MspA1I CMGCKG 1 cut(s) 350
MspR9I CCNGG 5 cut(s) 403, 1545, 1718, 1928, 1929
MvnI CGCG 2 cut(s) 183, 280
MwoI GCNNNNNNNGC 9 cut(s) 271, 350, 384, 1793, 1922, 2144, 2396, 2467, 2582
NciI CCSGG 5 cut(s) 403, 1545, 1718, 1928, 1929
NlaIV GGNNCC 7 cut(s) 207, 235, 236, 1265, 1926, 1933, 2310
NmeAIII GCCGAG 2 cut(s) 182, 369
NmuCI GTSAC 1 cut(s) 699
NsiI ATGCAT 1 cut(s) 1632
NspI RCATGY 2 cut(s) 1630, 2222
PaeI GCATGC 1 cut(s) 1630
PaeR7I CTCGAG 1 cut(s) 688
PagI TCATGA 2 cut(s) 1501, 2550
PciI ACATGT 1 cut(s) 2218
PciSI GCTCTTC 1 cut(s) 2424
PcsI WCGNNNNNNNCGW 3 cut(s) 209, 291, 1098
PdmI GAANNNNTTC 2 cut(s) 2076, 2236
PfeI GAWTC 9 cut(s) 105, 146, 551, 725, 908, 1525, 2096, 2150, 2179
PflFI GACNNNGTC 1 cut(s) 683
PkrI GCNGC 9 cut(s) 79, 156, 276, 279, 352, 1542, 1795, 1881, 2268
PleI GAGTC 8 cut(s) 5, 11, 920, 1031, 1685, 1718, 2126, 2128
PmaCI CACGTG 1 cut(s) 1313
PmlI CACGTG 1 cut(s) 1313
PpsI GAGTC 8 cut(s) 5, 11, 920, 1031, 1685, 1718, 2126, 2128
Ppu21I YACGTR 1 cut(s) 1313
PscI ACATGT 1 cut(s) 2218
PsiI TTATAA 1 cut(s) 2291
Psp124BI GAGCTC 2 cut(s) 564, 1941
PspCI CACGTG 1 cut(s) 1313
PspEI GGTNACC 1 cut(s) 699
PspN4I GGNNCC 7 cut(s) 207, 235, 236, 1265, 1926, 1933, 2310
PspOMI GGGCCC 1 cut(s) 233
PspPI GGNCC 6 cut(s) 164, 205, 233, 234, 1924, 2308
PstI CTGCAG 2 cut(s) 1795, 1855
PstNI CAGNNNCTG 1 cut(s) 45
PsuI RGATCY 4 cut(s) 1477, 1557, 2156, 2476
PsyI GACNNNGTC 1 cut(s) 683
RsaI GTAC 7 cut(s) 50, 461, 674, 749, 1644, 1755, 2520
RsaNI GTAC 7 cut(s) 49, 460, 673, 748, 1643, 1754, 2519
SacI GAGCTC 2 cut(s) 564, 1941
SapI GCTCTTC 1 cut(s) 2424
SaqAI TTAA 4 cut(s) 1085, 1382, 1461, 2105
SatI GCNGC 9 cut(s) 78, 155, 275, 278, 351, 1541, 1794, 1880, 2267
Sau96I GGNCC 6 cut(s) 164, 205, 233, 234, 1924, 2308
ScaI AGTACT 2 cut(s) 1755, 2520
SchI GAGTC 8 cut(s) 5, 11, 920, 1031, 1686, 1718, 2126, 2129
ScrFI CCNGG 5 cut(s) 403, 1545, 1718, 1928, 1929
SduI GDGCHC 6 cut(s) 237, 300, 564, 1268, 1941, 2533
SfaNI GCATC 8 cut(s) 969, 987, 1006, 1348, 1651, 1697, 1903, 2563
SfcI CTRYAG 3 cut(s) 555, 1791, 1851
Sfr274I CTCGAG 1 cut(s) 688
SinI GGWCC 2 cut(s) 164, 2308
SlaI CTCGAG 1 cut(s) 688
SmaI CCCGGG 1 cut(s) 1929
SmlI CTYRAG 1 cut(s) 688
SmoI CTYRAG 1 cut(s) 688
SphI GCATGC 1 cut(s) 1630
SsiI CCGC 9 cut(s) 62, 136, 155, 265, 275, 278, 348, 872, 1879
SspMI CTAG 1 cut(s) 1536
SstI GAGCTC 2 cut(s) 564, 1941
StyD4I CCNGG 5 cut(s) 401, 1543, 1716, 1926, 1927
StyI CCWWGG 3 cut(s) 1305, 1573, 2312
TaaI ACNGT 9 cut(s) 442, 631, 699, 752, 816, 1378, 1492, 1617, 2039
TaiI ACGT 2 cut(s) 899, 1315
TaqI TCGA 5 cut(s) 9, 689, 756, 2099, 2363
TatI WGTACW 4 cut(s) 672, 1642, 1753, 2518
TauI GCSGC 4 cut(s) 157, 277, 280, 1882
TfiI GAWTC 9 cut(s) 105, 146, 551, 725, 908, 1525, 2096, 2150, 2179
Tru1I TTAA 4 cut(s) 1085, 1382, 1461, 2105
Tru9I TTAA 4 cut(s) 1085, 1382, 1461, 2105
TscAI CASTG 4 cut(s) 459, 798, 819, 2391
TseFI GTSAC 1 cut(s) 699
TseI GCWGC 5 cut(s) 77, 350, 1540, 1793, 2266
Tsp45I GTSAC 1 cut(s) 699
TspDTI ATGAA 5 cut(s) 58, 361, 1764, 2238, 2529
TspGWI ACGGA 2 cut(s) 1353, 2477
TspMI CCCGGG 1 cut(s) 1927
TspRI CASTG 4 cut(s) 459, 798, 819, 2391
Tth111I GACNNNGTC 1 cut(s) 683
VpaK11BI GGWCC 2 cut(s) 164, 2308
XagI CCTNNNNNAGG 1 cut(s) 1289
XapI RAATTY 3 cut(s) 466, 2301, 2323
XceI RCATGY 2 cut(s) 1630, 2222
XhoI CTCGAG 1 cut(s) 688
XmaI CCCGGG 1 cut(s) 1927
XmiI GTMKAC 1 cut(s) 1822
XmnI GAANNNNTTC 2 cut(s) 2076, 2236
XspI CTAG 1 cut(s) 1536
ZrmI AGTACT 2 cut(s) 1755, 2520
Zsp2I ATGCAT 1 cut(s) 1632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.