Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0001000

Physical Location & Seq

Genomic Coordinates Reverse (-)

32647433 .. 32660174

Gene Structure 12742 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG328300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2529 bp

ATGCTTGATCGCAATGAAGAGTCGTCGAAGCCCGACAAGCCGGAAAGCCCACCGCAGCTGAAGAAATCACGGACTATAAGCGGCGACGATGCAACCCCAATGTTCCCGGGCCCACTGTTCCCAGCCGTTCGACGCATCTCCACCATTCCTTCCTCCCACAACTGGCCCCCGCCCGATCGCCACGCGTCGACTACTGCGGCGGACCACCGCGACTTCTCTGACCGTGACTGGGTCTTTCCGTCCTTTGTGCTTCCACAGAATCCTTCCAAACGAAGCAACAAGAAACACGCCTCCTCCGATGCATCCATCGCTTCTCAGCTCGCGGGGGCCACCGAAGCTGCTGCTGAGAGGAAGAAAATTAAGTTGGTGCCCCAGCCCGGACGTGAAGCACCTTCAACGAGCGAGTTGACTCGCCCGAGTTCCAGGACCCGGACTCGAGATTTGAAACCTTCTTTGTTGCTATTATTTTTGCTCACTTTCATTTGTATATTATCTGTACCTTATGCAGTTTATCTGCGGCAAAGAATTGCAAAACTTGAGGAATGTTGTATCAGTAAAAATGCTGGTACAGATGACAATATTGAGGCTTCACTGTCTGAGAAGAATCCAACAATTTTGCGTTTTCCTAATGCCGAAAGAAGAACTCTTGTTTTGTATTCTGTGGTCTTTACACTGGCCATGCCATTTGTGCTGTACAAACATCTTGATTATTTTTCCCATTTTACAATCCTCTCAAAAAACAAGAATAACAAAGAGGGGGTTCCCTTGAAGAAGAGGATAGCCTATATAGTGGATGTGTGCTTTTCTGTATATCCATATGCTAAACTACTTGCACTTCTCTTTGCAACAATATTACTAATAGGGTTTGGTGGACTGGCATTGTATGCAGTCAATAAAAGTAGCTTTGCTGAAGCTCTTTGGCTTTCATGGACTTTTGTTGCTGATTCTGGAAACCATGCTGATACAGAGGGCATTGGGCCAAGGATAGTTTCTGTCTCTATTAGTTCAGGAGGCATGCTGATATTTGCGATGATGCTTGGGCTTGTTTCTGATGCTATATCAGAGAAGGTTGACTCACTAAGGAAAGGGAAGAGTGAAGTCATTGAAAGGAACCACATACTCGTTCTTGGATGGAGTGATAAACTGGGTTCGCTTCTCAAGCAGCTTGCTATAGCAAACAAGAGTGTTGGTGGTGGTGTTGTTGTTGTACTTGCTGAAAGAGACAAGGAGGAAATGGAGATAGATATTGCAAAACTTGAATTTGATTTCATGGGTACGTCTGTTATATGCAGAAGTGGCAGTCCTCTTATACTTGCTGACCTAAAGAAGGTCTCAGTTTCGAAGGCGCGTGCTATCATTGTATTGGCATCCGATGAAAATGCAGATCAGAGTGATGCACGTGCTCTGAGGGTTGTTCTAAGCCTCACTGGGGTGAAAGAGGGTTTGAGGGGACATGTTGTTGTAGAGATGAGTGACCTGGATAATGAGCCTCTGGTGAAGCTTGTTGGAGGAGAACTCATTGAAACAGTAGTAGCACATGATGTGATTGGGCGGTTAATGATACAGTGTGCTCTACAGCCTGGTCTTGCGCAGATATGGGAGGATATATTGGGATTTGAGAATGCGGAGTTTTACATCAAAAGGTGGCCTCAGTTGGATGGTCTTCGTTTCGAAAATGTGCTCATTTCATTTCCTGATGCAATTCCCTGTGGAATCAAGGTTGCCGCTGATGGTGGAAAGATAAATTTAAATCCCGATGATAAATATGTTATGAAAGAAGGGGATGAAGTTCTTGTTATAGCTGAAGATGACGACACTTATGCTCCATCTGCCTTTCCAGAGCAGGTATGTGCTGGCCTTTGTCCAAACACTGTTGAACCTCCCAAATACCCTGAGAAGATATTGTTCTGTGGCTGGCGTCGTGACATAGACGATATGATACTGGTTCTAGAGGCATTCTTGCCTCTAGGTTCAGAACTTTGGATGTTCAATGAGGTTCCAGAAAAAGAAAGAGAGAAGAAACTTACAGATGGTGGACTTGATATTTCGCGATTGGAGAACATAAAACTCGTTCACAAAGAAGGAAATGCTGTGATTAGACGGCATCTAGAGAATCTTCCCTTGGAGACTTTTGATTCTATATTAATTCTTGCAGATGAATCACTAGAGGACTCTGTTGTGCATTCTGACTCGAGATCCCTTGCCACTCTTCTCCTTATACGCGATATACAGTCAAAGCGTCTTCCTTACAAAGAAACAAAGTCAACTTCCTTATCTGCATTTTCGCATAGCTCTTGGATTCGTGAAATGCAGCAAGCTTCAGACAAATCGATAATTATAAGTGAAATTCTGGATTCTAGGACTAGAAACCTGGTCTCTGTTTCCAGAATCAGTGATTATGTGCTGTCAAATGAACTGGTTAGTATGGCACTGGCAATGGTGGCTGAAGATAAGCAAATCAATCGCGTTCTTGAGGAGCTATTTGCGGAGGAGGTACTCTCCAGTCTTTCAGGAATTTATGTTATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Castor_Poll_mid

Protein Analysis

842

Amino Acids

93.27

Weight (kDa)

5.56

Isoelectric Point (pI)

48.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Slo-like_RCK	PF22614	370 - 474	2.2e-07	Calcium-activated potassium channel slowpoke-like RCK domain
Castor_Poll_mid	PF06241	513 - 611	1.6e-42	Castor and Pollux, part of voltage-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001000)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49960 AT5G49960 AT5G49960
fragaria_vesca	FvH4_4g10120 FvH4_4g10120 FvH4_4g10120 FvH4_4g10120 FvH4_4g10120 FvH4_4g10120 FvH4_4g10120 FvH4_6g26000 FvH4_6g26000 FvH4_6g26000 FvH4_6g26000 FvH4_6g26000 FvH4_6g26000 FvH4_6g26000 FvH4_6g26000 FvH4_6g26000 FvH4_6g26000 FvH4_6g26000 FvH4_6g26000 FvH4_6g26000 FvH4_6g26000 FvH4_6g26000 FvH4_6g26000 FvH4_6g26000
malus_domestica	MD00G1064100.v1.1 MD08G1240900.v1.1
prunus_persica	Prupe.1G102500_v2.0.a1 Prupe.1G102500_v2.0.a1 Prupe.1G102500_v2.0.a1 Prupe.1G102500_v2.0.a1 Prupe.1G577700_v2.0.a1
pyrus_communis	pycom08g20880 pycom16g22540
rosa_chinensis	RchiOBHm_Chr4g0405621 RchiOBHm_Chr7g0220721
rosa_laevigata	RLG00000002201 RLG00000008832
rosa_multiflora	Rmu_sc0001200.1_g000056 Rmu_sc0001286.1_g000017
rosa_roxburghii	Rroxscaffold_3G00239110 Rroxscaffold_5G00350110
rosa_rugosa	Rorug04G0060900 Rorug04G0061000 Rorug04G0061100 Rorug07G0196900 Rorug07G0196900 Rorug07G0196900 Rorug07G0196900
rosa_samantha	Rh4AG132800 Rh4BG127600 Rh4CG140500 Rh4DG127200 Rh7AG337400 Rh7BG328300 Rh7CG355500 Rh7DG334100
rosa_wichuraiana	Rw4G010750 Rw4G010760 Rw7G028790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2339
Acc16I	TGCGCA	1 cut(s)	1590
Acc36I	ACCTGC	1 cut(s)	1834
AccB1I	GGYRCC	1 cut(s)	367
AccI	GTMKAC	1 cut(s)	188
AccII	CGCG	7 cut(s)	185, 210, 323, 1348, 2050, 2223, 2466
AclWI	GGATC	1 cut(s)	2190
AcoI	YGGCCR	1 cut(s)	675
AcsI	RAATTY	4 cut(s)	1259, 1744, 2346, 2514
AcuI	CTGAAG	5 cut(s)	80, 930, 1824, 2304, 2466
AcvI	CACGTG	1 cut(s)	1400
AcyI	GRCGYC	1 cut(s)	1918
AfaI	GTAC	6 cut(s)	498, 568, 695, 1209, 1276, 2496
AfiI	CCNNNNNNNGG	6 cut(s)	162, 229, 377, 429, 1327, 1429
AflIII	ACRYGT	2 cut(s)	183, 1453
AgsI	TTSAA	8 cut(s)	396, 445, 769, 1106, 1259, 1523, 1877, 1990
AjiI	CACGTC	1 cut(s)	383
AjnI	CCWGG	4 cut(s)	422, 1476, 1579, 2370
AjuI	GAANNNNNNNTTGG	6 cut(s)	347, 379, 973, 1005, 2105, 2137
AleI	CACNNNNGTG	1 cut(s)	1430
Alw21I	GWGCWC	3 cut(s)	1405, 1573, 1683
Alw26I	GTCTC	5 cut(s)	1000, 1215, 1336, 2120, 2380
AlwI	GGATC	1 cut(s)	2190
Ama87I	CYCGRG	4 cut(s)	106, 415, 435, 2191
AoxI	GGCC	7 cut(s)	109, 164, 327, 675, 977, 1646, 1855
ApaI	GGGCCC	1 cut(s)	113
ApeKI	GCWGC	5 cut(s)	55, 338, 341, 1162, 2311
ApoI	RAATTY	4 cut(s)	1259, 1744, 2346, 2514
ArsI	GACNNNNNNTTYG	2 cut(s)	424, 456
AseI	ATTAAT	1 cut(s)	2144
AspLEI	GCGC	2 cut(s)	1348, 1591
AspS9I	GGNCC	7 cut(s)	109, 110, 165, 202, 327, 426, 977
AsuC2I	CCSGG	4 cut(s)	107, 108, 378, 430
AsuHPI	GGTGA	2 cut(s)	1444, 1507
AsuII	TTCGAA	2 cut(s)	1340, 1671
AvaI	CYCGRG	4 cut(s)	106, 415, 435, 2191
AvaII	GGWCC	2 cut(s)	202, 426
BaeGI	GKGCMC	2 cut(s)	113, 372
BaeI	ACNNNNGTAYC	2 cut(s)	1266, 1299
BalI	TGGCCA	1 cut(s)	677
BanI	GGYRCC	1 cut(s)	367
BanII	GRGCYC	1 cut(s)	113
BbrPI	CACGTG	1 cut(s)	1400
BbsI	GAAGAC	2 cut(s)	1655, 2234
Bbv12I	GWGCWC	3 cut(s)	1405, 1573, 1683
BbvI	GCAGC	5 cut(s)	67, 325, 328, 1174, 2323
BccI	CCATC	6 cut(s)	314, 1125, 1652, 1724, 1834, 2024
BceAI	ACGGC	2 cut(s)	110, 2117
BcgI	CGANNNNNNTGC	8 cut(s)	323, 357, 1361, 1395, 1802, 1836, 2444, 2478
BciT130I	CCWGG	4 cut(s)	424, 1478, 1581, 2372
BcnI	CCSGG	4 cut(s)	107, 108, 378, 430
BcoDI	GTCTC	5 cut(s)	1000, 1215, 1336, 2120, 2380
BfaI	CTAG	6 cut(s)	1949, 1967, 2108, 2165, 2358, 2364
BfmI	CTRYAG	2 cut(s)	1170, 1574
BfuAI	ACCTGC	1 cut(s)	1834
BisI	GCNGC	9 cut(s)	56, 82, 198, 339, 342, 518, 1163, 1725, 2312
BlsI	GCNGC	9 cut(s)	57, 83, 199, 340, 343, 519, 1164, 1726, 2313
Bme1390I	CCNGG	8 cut(s)	107, 108, 378, 424, 430, 1478, 1581, 2372
Bme18I	GGWCC	2 cut(s)	202, 426
BmeT110I	CYCGRG	4 cut(s)	106, 415, 435, 2191
BmgBI	CACGTC	1 cut(s)	383
BmgT120I	GGNCC	7 cut(s)	109, 110, 165, 202, 327, 426, 977
BmiI	GGNNCC	8 cut(s)	111, 167, 328, 369, 428, 762, 1112, 1998
BmrFI	CCNGG	8 cut(s)	107, 108, 378, 424, 430, 1478, 1581, 2372
BmrI	ACTGGG	3 cut(s)	238, 1154, 1437
BmuI	ACTGGG	3 cut(s)	238, 1154, 1437
BpiI	GAAGAC	2 cut(s)	1655, 2234
BplI	GAGNNNNNCTC	4 cut(s)	1500, 1532, 2483, 2515
BpmI	CTGGAG	1 cut(s)	2485
Bpu14I	TTCGAA	2 cut(s)	1340, 1671
BpuEI	CTTGAG	3 cut(s)	557, 1142, 2492
BpuMI	CCSGG	4 cut(s)	107, 108, 378, 430
Bsa29I	ATCGAT	1 cut(s)	2330
BsaAI	YACGTR	1 cut(s)	1400
BsaHI	GRCGYC	1 cut(s)	1918
BsaI	GGTCTC	2 cut(s)	1336, 2380
BsaJI	CCNNGG	3 cut(s)	106, 980, 2121
BsaXI	ACNNNNNCTCC	4 cut(s)	278, 308, 1807, 1837
Bsc4I	CCNNNNNNNGG	6 cut(s)	162, 229, 377, 429, 1327, 1429
Bse3DI	GCAATG	2 cut(s)	19, 2442
BseBI	CCWGG	4 cut(s)	424, 1478, 1581, 2372
BseCI	ATCGAT	1 cut(s)	2330
BseDI	CCNNGG	3 cut(s)	106, 980, 2121
BseGI	GGATG	7 cut(s)	302, 799, 1136, 1367, 1663, 1789, 1989
BseLI	CCNNNNNNNGG	6 cut(s)	162, 229, 377, 429, 1327, 1429
BseMI	GCAATG	2 cut(s)	19, 2442
BseMII	CTCAG	7 cut(s)	329, 336, 588, 1347, 1397, 1664, 1884
BseRI	GAGGAG	4 cut(s)	283, 1524, 2489, 2504
BseSI	GKGCMC	2 cut(s)	113, 372
BseXI	GCAGC	5 cut(s)	67, 325, 328, 1174, 2323
BseYI	CCCAGC	2 cut(s)	121, 372
Bsh1236I	CGCG	7 cut(s)	185, 210, 323, 1348, 2050, 2223, 2466
Bsh1285I	CGRYCG	1 cut(s)	178
BshFI	GGCC	7 cut(s)	111, 166, 329, 677, 979, 1648, 1857
BshNI	GGYRCC	1 cut(s)	367
BshVI	ATCGAT	1 cut(s)	2330
BsiEI	CGRYCG	1 cut(s)	178
BsiHKAI	GWGCWC	3 cut(s)	1405, 1573, 1683
BsiHKCI	CYCGRG	4 cut(s)	106, 415, 435, 2191
BsiSI	CCGG	4 cut(s)	41, 107, 378, 430
BslFI	GGGAC	1 cut(s)	1464
BslI	CCNNNNNNNGG	6 cut(s)	162, 229, 377, 429, 1327, 1429
BsmAI	GTCTC	5 cut(s)	1000, 1215, 1336, 2120, 2380
BsmFI	GGGAC	1 cut(s)	1464
BsmI	GAATGC	3 cut(s)	1627, 1955, 2182
BsnI	GGCC	7 cut(s)	111, 166, 329, 677, 979, 1648, 1857
Bso31I	GGTCTC	2 cut(s)	1336, 2380
BsoBI	CYCGRG	4 cut(s)	106, 415, 435, 2191
Bsp119I	TTCGAA	2 cut(s)	1340, 1671
Bsp120I	GGGCCC	1 cut(s)	109
Bsp1286I	GDGCHC	5 cut(s)	113, 372, 1405, 1573, 1683
Bsp1407I	TGTACA	1 cut(s)	693
Bsp143I	GATC	4 cut(s)	7, 175, 1384, 2195
Bsp68I	TCGCGA	1 cut(s)	2050
BspANI	GGCC	7 cut(s)	111, 166, 329, 677, 979, 1648, 1857
BspCNI	CTCAG	7 cut(s)	328, 337, 589, 1346, 1398, 1663, 1885
BspDI	ATCGAT	1 cut(s)	2330
BspFNI	CGCG	7 cut(s)	185, 210, 323, 1348, 2050, 2223, 2466
BspLI	GGNNCC	8 cut(s)	111, 167, 328, 369, 428, 762, 1112, 1998
BspMI	ACCTGC	1 cut(s)	1834
BspPI	GGATC	1 cut(s)	2190
BspT104I	TTCGAA	2 cut(s)	1340, 1671
BspT107I	GGYRCC	1 cut(s)	367
BspTNI	GGTCTC	2 cut(s)	1336, 2380
BsrDI	GCAATG	2 cut(s)	19, 2442
BsrGI	TGTACA	1 cut(s)	693
BssECI	CCNNGG	3 cut(s)	106, 980, 2121
BssMI	GATC	4 cut(s)	7, 175, 1384, 2195
BssNI	GRCGYC	1 cut(s)	1918
BssT1I	CCWWGG	2 cut(s)	980, 2121
Bst2UI	CCWGG	4 cut(s)	424, 1478, 1581, 2372
Bst4CI	ACNGT	7 cut(s)	117, 224, 594, 1528, 1566, 1873, 2232
Bst6I	CTCTTC	4 cut(s)	12, 767, 1085, 2214
BstACI	GRCGYC	1 cut(s)	1918
BstAPI	GCANNNNNTGC	1 cut(s)	884
BstAUI	TGTACA	1 cut(s)	693
BstBAI	YACGTR	1 cut(s)	1400
BstBI	TTCGAA	2 cut(s)	1340, 1671
BstC8I	GCNNGC	7 cut(s)	321, 1016, 1167, 1350, 1855, 1916, 2316
BstDEI	CTNAG	9 cut(s)	315, 345, 597, 1079, 1333, 1406, 1418, 1650, 1893
BstENI	CCTNNNNNAGG	1 cut(s)	1325
BstF5I	GGATG	7 cut(s)	302, 799, 1136, 1367, 1663, 1789, 1989
BstFNI	CGCG	7 cut(s)	185, 210, 323, 1348, 2050, 2223, 2466
BstHHI	GCGC	2 cut(s)	1348, 1591
BstKTI	GATC	4 cut(s)	10, 178, 1387, 2198
BstMAI	GTCTC	5 cut(s)	1000, 1215, 1336, 2120, 2380
BstMBI	GATC	4 cut(s)	7, 175, 1384, 2195
BstMCI	CGRYCG	1 cut(s)	178
BstMWI	GCNNNNNNNGC	9 cut(s)	37, 308, 335, 688, 884, 1159, 1296, 1829, 2441
BstNI	CCWGG	4 cut(s)	424, 1478, 1581, 2372
BstNSI	RCATGY	2 cut(s)	1018, 1457
BstSCI	CCNGG	8 cut(s)	105, 106, 376, 422, 428, 1476, 1579, 2370
BstSFI	CTRYAG	2 cut(s)	1170, 1574
BstSLI	GKGCMC	2 cut(s)	113, 372
BstUI	CGCG	7 cut(s)	185, 210, 323, 1348, 2050, 2223, 2466
BstV1I	GCAGC	5 cut(s)	67, 325, 328, 1174, 2323
BstV2I	GAAGAC	2 cut(s)	1655, 2234
BstX2I	RGATCY	1 cut(s)	2195
BstYI	RGATCY	1 cut(s)	2195
Bsu15I	ATCGAT	1 cut(s)	2330
BsuRI	GGCC	7 cut(s)	111, 166, 329, 677, 979, 1648, 1857
BsuTUI	ATCGAT	1 cut(s)	2330
BtgZI	GCGATG	2 cut(s)	292, 1043
BtrI	CACGTC	1 cut(s)	383
BtsCI	GGATG	7 cut(s)	302, 799, 1136, 1367, 1663, 1789, 1989
BtsIMutI	CAGTG	8 cut(s)	113, 590, 671, 1425, 1571, 1869, 2398, 2429
BtuMI	TCGCGA	1 cut(s)	2050
BveI	ACCTGC	1 cut(s)	1834
Cac8I	GCNNGC	7 cut(s)	321, 1016, 1167, 1350, 1855, 1916, 2316
CfoI	GCGC	2 cut(s)	1348, 1591
Cfr13I	GGNCC	7 cut(s)	109, 110, 165, 202, 327, 426, 977
Cfr9I	CCCGGG	1 cut(s)	106
ClaI	ATCGAT	1 cut(s)	2330
CseI	GACGC	4 cut(s)	141, 174, 1907, 2228
CsiI	ACCWGGT	1 cut(s)	2370
Csp6I	GTAC	6 cut(s)	497, 567, 694, 1208, 1275, 2495
CviAII	CATG	7 cut(s)	679, 927, 956, 1015, 1270, 1454, 1538
CviQI	GTAC	6 cut(s)	497, 567, 694, 1208, 1275, 2495
DdeI	CTNAG	9 cut(s)	315, 345, 597, 1079, 1333, 1406, 1418, 1650, 1893
DpnI	GATC	4 cut(s)	9, 177, 1386, 2197
DpnII	GATC	4 cut(s)	7, 175, 1384, 2195
DraI	TTTAAA	1 cut(s)	1749
EaeI	YGGCCR	1 cut(s)	675
Eam1104I	CTCTTC	4 cut(s)	12, 767, 1085, 2214
EarI	CTCTTC	4 cut(s)	12, 767, 1085, 2214
EciI	GGCGGA	1 cut(s)	215
Eco130I	CCWWGG	2 cut(s)	980, 2121
Eco24I	GRGCYC	1 cut(s)	113
Eco31I	GGTCTC	2 cut(s)	1336, 2380
Eco47I	GGWCC	2 cut(s)	202, 426
Eco57I	CTGAAG	5 cut(s)	80, 930, 1824, 2304, 2466
Eco72I	CACGTG	1 cut(s)	1400
Eco88I	CYCGRG	4 cut(s)	106, 415, 435, 2191
EcoNI	CCTNNNNNAGG	1 cut(s)	1325
EcoO109I	RGGNCCY	1 cut(s)	426
EcoRII	CCWGG	4 cut(s)	422, 1476, 1579, 2370
EcoT14I	CCWWGG	2 cut(s)	980, 2121
EcoT22I	ATGCAT	1 cut(s)	304
EcoT38I	GRGCYC	1 cut(s)	113
ErhI	CCWWGG	2 cut(s)	980, 2121
FaeI	CATG	7 cut(s)	682, 930, 959, 1018, 1273, 1457, 1541
FaqI	GGGAC	1 cut(s)	1464
FatI	CATG	7 cut(s)	678, 926, 955, 1014, 1269, 1453, 1537
FauI	CCCGC	2 cut(s)	177, 316
FauNDI	CATATG	1 cut(s)	817
FblI	GTMKAC	1 cut(s)	188
Fnu4HI	GCNGC	9 cut(s)	56, 82, 198, 339, 342, 518, 1163, 1725, 2312
FokI	GGATG	7 cut(s)	289, 806, 1143, 1354, 1670, 1796, 1996
FriOI	GRGCYC	1 cut(s)	113
Fsp4HI	GCNGC	9 cut(s)	56, 82, 198, 339, 342, 518, 1163, 1725, 2312
FspBI	CTAG	6 cut(s)	1949, 1967, 2108, 2165, 2358, 2364
FspI	TGCGCA	1 cut(s)	1590
GlaI	GCGC	2 cut(s)	1347, 1590
GluI	GCNGC	9 cut(s)	56, 82, 198, 339, 342, 518, 1163, 1725, 2312
GsaI	CCCAGC	2 cut(s)	125, 376
GsuI	CTGGAG	1 cut(s)	2485
HaeIII	GGCC	7 cut(s)	111, 166, 329, 677, 979, 1648, 1857
HapII	CCGG	4 cut(s)	41, 107, 378, 430
HgaI	GACGC	4 cut(s)	141, 174, 1907, 2228
HhaI	GCGC	2 cut(s)	1348, 1591
Hin1I	GRCGYC	1 cut(s)	1918
Hin1II	CATG	7 cut(s)	682, 930, 959, 1018, 1273, 1457, 1541
Hin6I	GCGC	2 cut(s)	1346, 1589
HinP1I	GCGC	2 cut(s)	1346, 1589
HincII	GTYRAC	4 cut(s)	189, 408, 1072, 2265
HindII	GTYRAC	4 cut(s)	189, 408, 1072, 2265
HindIII	AAGCTT	2 cut(s)	1499, 2316
HpaII	CCGG	4 cut(s)	41, 107, 378, 430
HphI	GGTGA	2 cut(s)	1444, 1507
Hpy166II	GTNNAC	7 cut(s)	189, 408, 872, 1072, 2036, 2074, 2265
Hpy8I	GTNNAC	7 cut(s)	189, 408, 872, 1072, 2036, 2074, 2265
Hpy99I	CGWCG	5 cut(s)	28, 89, 135, 190, 1923
HpyAV	CCTTC	9 cut(s)	159, 273, 402, 459, 1060, 1321, 1336, 1772, 2075
HpyCH4III	ACNGT	7 cut(s)	117, 224, 594, 1528, 1566, 1873, 2232
HpyCH4IV	ACGT	3 cut(s)	382, 1277, 1399
HpyF10VI	GCNNNNNNNGC	9 cut(s)	37, 308, 335, 688, 884, 1159, 1296, 1829, 2441
HpyF3I	CTNAG	9 cut(s)	315, 345, 597, 1079, 1333, 1406, 1418, 1650, 1893
HpySE526I	ACGT	3 cut(s)	382, 1277, 1399
Hsp92I	GRCGYC	1 cut(s)	1918
Hsp92II	CATG	7 cut(s)	682, 930, 959, 1018, 1273, 1457, 1541
HspAI	GCGC	2 cut(s)	1346, 1589
Kzo9I	GATC	4 cut(s)	7, 175, 1384, 2195
LmnI	GCTCC	2 cut(s)	1828, 2476
Lsp1109I	GCAGC	5 cut(s)	67, 325, 328, 1174, 2323
MabI	ACCWGGT	1 cut(s)	2370
MaeI	CTAG	6 cut(s)	1949, 1967, 2108, 2165, 2358, 2364
MaeII	ACGT	3 cut(s)	382, 1277, 1399
MaeIII	GTNAC	3 cut(s)	224, 1472, 1922
MalI	GATC	4 cut(s)	9, 177, 1386, 2197
MboI	GATC	4 cut(s)	7, 175, 1384, 2195
MflI	RGATCY	1 cut(s)	2195
MhlI	GDGCHC	5 cut(s)	113, 372, 1405, 1573, 1683
MlsI	TGGCCA	1 cut(s)	677
MluI	ACGCGT	1 cut(s)	183
MluNI	TGGCCA	1 cut(s)	677
MlyI	GAGTC	6 cut(s)	29, 403, 427, 1067, 2165, 2183
MmeI	TCCRAC	3 cut(s)	632, 1486, 1635
Mox20I	TGGCCA	1 cut(s)	677
Mph1103I	ATGCAT	1 cut(s)	304
MscI	TGGCCA	1 cut(s)	677
MseI	TTAA	4 cut(s)	360, 1556, 1748, 2144
MslI	CAYNNNNRTG	1 cut(s)	1430
Msp20I	TGGCCA	1 cut(s)	677
MspA1I	CMGCKG	2 cut(s)	58, 1727
MspI	CCGG	4 cut(s)	41, 107, 378, 430
MspR9I	CCNGG	8 cut(s)	107, 108, 378, 424, 430, 1478, 1581, 2372
Mva1269I	GAATGC	3 cut(s)	1627, 1955, 2182
MvaI	CCWGG	4 cut(s)	424, 1478, 1581, 2372
MvnI	CGCG	7 cut(s)	185, 210, 323, 1348, 2050, 2223, 2466
MwoI	GCNNNNNNNGC	9 cut(s)	37, 308, 335, 688, 884, 1159, 1296, 1829, 2441
NciI	CCSGG	4 cut(s)	107, 108, 378, 430
NdeI	CATATG	1 cut(s)	817
NdeII	GATC	4 cut(s)	7, 175, 1384, 2195
NlaIII	CATG	7 cut(s)	682, 930, 959, 1018, 1273, 1457, 1541
NlaIV	GGNNCC	8 cut(s)	111, 167, 328, 369, 428, 762, 1112, 1998
NmuCI	GTSAC	3 cut(s)	224, 1472, 1922
NruI	TCGCGA	1 cut(s)	2050
NsbI	TGCGCA	1 cut(s)	1590
NsiI	ATGCAT	1 cut(s)	304
NspI	RCATGY	2 cut(s)	1018, 1457
NspV	TTCGAA	2 cut(s)	1340, 1671
OliI	CACNNNNGTG	1 cut(s)	1430
PaeI	GCATGC	1 cut(s)	1018
PaeR7I	CTCGAG	2 cut(s)	435, 2191
PciI	ACATGT	1 cut(s)	1453
PcsI	WCGNNNNNNNCGW	1 cut(s)	294
PctI	GAATGC	3 cut(s)	1627, 1955, 2182
PflFI	GACNNNGTC	1 cut(s)	230
PfoI	TCCNGGA	1 cut(s)	422
PkrI	GCNGC	9 cut(s)	57, 83, 199, 340, 343, 519, 1164, 1726, 2313
Ple19I	CGATCG	1 cut(s)	178
PleI	GAGTC	6 cut(s)	28, 403, 427, 1067, 2165, 2183
PmaCI	CACGTG	1 cut(s)	1400
PmlI	CACGTG	1 cut(s)	1400
PpsI	GAGTC	6 cut(s)	28, 403, 427, 1067, 2165, 2183
Ppu21I	YACGTR	1 cut(s)	1400
PpuMI	RGGWCCY	1 cut(s)	426
PscI	ACATGT	1 cut(s)	1453
PshBI	ATTAAT	1 cut(s)	2144
PsiI	TTATAA	1 cut(s)	2339
Psp5II	RGGWCCY	1 cut(s)	426
Psp6I	CCWGG	4 cut(s)	422, 1476, 1579, 2370
PspCI	CACGTG	1 cut(s)	1400
PspFI	CCCAGC	2 cut(s)	121, 372
PspGI	CCWGG	4 cut(s)	422, 1476, 1579, 2370
PspN4I	GGNNCC	8 cut(s)	111, 167, 328, 369, 428, 762, 1112, 1998
PspOMI	GGGCCC	1 cut(s)	109
PspPI	GGNCC	7 cut(s)	109, 110, 165, 202, 327, 426, 977
PspPPI	RGGWCCY	1 cut(s)	426
PsuI	RGATCY	1 cut(s)	2195
PsyI	GACNNNGTC	1 cut(s)	230
PvuI	CGATCG	1 cut(s)	178
PvuII	CAGCTG	1 cut(s)	58
RruI	TCGCGA	1 cut(s)	2050
RsaI	GTAC	6 cut(s)	498, 568, 695, 1209, 1276, 2496
RsaNI	GTAC	6 cut(s)	497, 567, 694, 1208, 1275, 2495
RseI	CAYNNNNRTG	1 cut(s)	1430
SalI	GTCGAC	1 cut(s)	187
SaqAI	TTAA	4 cut(s)	360, 1556, 1748, 2144
SatI	GCNGC	9 cut(s)	56, 82, 198, 339, 342, 518, 1163, 1725, 2312
Sau3AI	GATC	4 cut(s)	7, 175, 1384, 2195
Sau96I	GGNCC	7 cut(s)	109, 110, 165, 202, 327, 426, 977
SchI	GAGTC	6 cut(s)	29, 403, 427, 1067, 2165, 2183
ScrFI	CCNGG	8 cut(s)	107, 108, 378, 424, 430, 1478, 1581, 2372
SduI	GDGCHC	5 cut(s)	113, 372, 1405, 1573, 1683
SexAI	ACCWGGT	1 cut(s)	2370
SfcI	CTRYAG	2 cut(s)	1170, 1574
Sfr274I	CTCGAG	2 cut(s)	435, 2191
SfuI	TTCGAA	2 cut(s)	1340, 1671
SinI	GGWCC	2 cut(s)	202, 426
SlaI	CTCGAG	2 cut(s)	435, 2191
SmaI	CCCGGG	1 cut(s)	108
SmiI	ATTTAAAT	1 cut(s)	1749
SmiMI	CAYNNNNRTG	1 cut(s)	1430
SmlI	CTYRAG	5 cut(s)	435, 536, 1157, 2191, 2471
SmoI	CTYRAG	5 cut(s)	435, 536, 1157, 2191, 2471
SphI	GCATGC	1 cut(s)	1018
SspI	AATATT	2 cut(s)	580, 852
SspMI	CTAG	6 cut(s)	1949, 1967, 2108, 2165, 2358, 2364
StyD4I	CCNGG	8 cut(s)	105, 106, 376, 422, 428, 1476, 1579, 2370
StyI	CCWWGG	2 cut(s)	980, 2121
SwaI	ATTTAAAT	1 cut(s)	1749
TaaI	ACNGT	7 cut(s)	117, 224, 594, 1528, 1566, 1873, 2232
TaiI	ACGT	3 cut(s)	385, 1280, 1402
TaqI	TCGA	8 cut(s)	26, 130, 188, 436, 1340, 1671, 2192, 2330
TatI	WGTACW	2 cut(s)	693, 1207
TauI	GCSGC	4 cut(s)	84, 200, 520, 1727
Tru1I	TTAA	4 cut(s)	360, 1556, 1748, 2144
Tru9I	TTAA	4 cut(s)	360, 1556, 1748, 2144
TscAI	CASTG	8 cut(s)	120, 597, 678, 1432, 1571, 1876, 2398, 2436
TseFI	GTSAC	3 cut(s)	224, 1472, 1922
TseI	GCWGC	5 cut(s)	55, 338, 341, 1162, 2311
Tsp45I	GTSAC	3 cut(s)	224, 1472, 1922
TspGWI	ACGGA	2 cut(s)	85, 228
TspMI	CCCGGG	1 cut(s)	106
TspRI	CASTG	8 cut(s)	120, 597, 678, 1432, 1571, 1876, 2398, 2436
Tth111I	GACNNNGTC	1 cut(s)	230
VpaK11BI	GGWCC	2 cut(s)	202, 426
VspI	ATTAAT	1 cut(s)	2144
XagI	CCTNNNNNAGG	1 cut(s)	1325
XapI	RAATTY	4 cut(s)	1259, 1744, 2346, 2514
XbaI	TCTAGA	2 cut(s)	1948, 2107
XceI	RCATGY	2 cut(s)	1018, 1457
XhoI	CTCGAG	2 cut(s)	435, 2191
XmaI	CCCGGG	1 cut(s)	106
XmiI	GTMKAC	1 cut(s)	188
XspI	CTAG	6 cut(s)	1949, 1967, 2108, 2165, 2358, 2364
Zsp2I	ATGCAT	1 cut(s)	304

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.