Ion channel
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2456 |
| Acc16I | TGCGCA | 1 cut(s) | 1587 |
| AccB1I | GGYRCC | 2 cut(s) | 364, 422 |
| AccBSI | CCGCTC | 1 cut(s) | 2743 |
| AccI | GTMKAC | 1 cut(s) | 188 |
| AccII | CGCG | 4 cut(s) | 185, 210, 2167, 2583 |
| AclWI | GGATC | 1 cut(s) | 2307 |
| AcoI | YGGCCR | 1 cut(s) | 672 |
| AcsI | RAATTY | 3 cut(s) | 1256, 1864, 2463 |
| AcuI | CTGAAG | 6 cut(s) | 80, 927, 1659, 1944, 2421, 2583 |
| AcvI | CACGTG | 2 cut(s) | 1346, 1397 |
| AcyI | GRCGYC | 1 cut(s) | 2035 |
| AfaI | GTAC | 5 cut(s) | 495, 565, 692, 1206, 2778 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 162, 229, 319, 374, 419, 426, 1324, 1426, 2097 |
| AflIII | ACRYGT | 2 cut(s) | 183, 1450 |
| AgsI | TTSAA | 9 cut(s) | 393, 442, 766, 1103, 1256, 1520, 1605, 1994, 2107 |
| AjiI | CACGTC | 1 cut(s) | 380 |
| AjnI | CCWGG | 4 cut(s) | 1473, 1576, 2487, 2655 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 344, 376, 970, 1002, 2700, 2732 |
| AleI | CACNNNNGTG | 1 cut(s) | 1427 |
| Alw21I | GWGCWC | 7 cut(s) | 252, 419, 1402, 1570, 1618, 1803, 2667 |
| Alw26I | GTCTC | 6 cut(s) | 997, 1212, 1333, 2237, 2497, 2793 |
| AlwI | GGATC | 1 cut(s) | 2307 |
| Ama87I | CYCGRG | 5 cut(s) | 106, 412, 432, 2009, 2308 |
| AoxI | GGCC | 8 cut(s) | 109, 164, 324, 672, 974, 1766, 1972, 2745 |
| ApaI | GGGCCC | 1 cut(s) | 113 |
| ApeKI | GCWGC | 7 cut(s) | 55, 276, 335, 338, 1159, 1844, 2428 |
| ApoI | RAATTY | 3 cut(s) | 1256, 1864, 2463 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 421, 453 |
| AseI | ATTAAT | 1 cut(s) | 2261 |
| AspLEI | GCGC | 1 cut(s) | 1588 |
| AspS9I | GGNCC | 7 cut(s) | 109, 110, 165, 202, 324, 974, 2745 |
| AsuC2I | CCSGG | 4 cut(s) | 107, 108, 375, 427 |
| AsuHPI | GGTGA | 3 cut(s) | 1441, 1504, 1654 |
| AsuII | TTCGAA | 2 cut(s) | 1337, 1791 |
| AvaI | CYCGRG | 5 cut(s) | 106, 412, 432, 2009, 2308 |
| AvaII | GGWCC | 1 cut(s) | 202 |
| BaeGI | GKGCMC | 2 cut(s) | 113, 369 |
| BalI | TGGCCA | 1 cut(s) | 674 |
| BanI | GGYRCC | 2 cut(s) | 364, 422 |
| BanII | GRGCYC | 3 cut(s) | 113, 419, 2667 |
| BbrPI | CACGTG | 2 cut(s) | 1346, 1397 |
| BbsI | GAAGAC | 3 cut(s) | 1775, 2225, 2351 |
| Bbv12I | GWGCWC | 7 cut(s) | 252, 419, 1402, 1570, 1618, 1803, 2667 |
| BbvI | GCAGC | 7 cut(s) | 67, 288, 322, 325, 1171, 1831, 2440 |
| BccI | CCATC | 7 cut(s) | 311, 1122, 1772, 1844, 1954, 2141, 2767 |
| BceAI | ACGGC | 4 cut(s) | 110, 210, 2234, 2732 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 320, 354, 1922, 1956, 2561, 2595 |
| BciT130I | CCWGG | 4 cut(s) | 1475, 1578, 2489, 2657 |
| BcnI | CCSGG | 4 cut(s) | 107, 108, 375, 427 |
| BcoDI | GTCTC | 6 cut(s) | 997, 1212, 1333, 2237, 2497, 2793 |
| BfaI | CTAG | 6 cut(s) | 2066, 2084, 2225, 2282, 2475, 2481 |
| BfmI | CTRYAG | 4 cut(s) | 1167, 1571, 1623, 1845 |
| Bme1390I | CCNGG | 8 cut(s) | 107, 108, 375, 427, 1475, 1578, 2489, 2657 |
| Bme18I | GGWCC | 1 cut(s) | 202 |
| BmeT110I | CYCGRG | 5 cut(s) | 106, 412, 432, 2009, 2308 |
| BmgBI | CACGTC | 1 cut(s) | 380 |
| BmgT120I | GGNCC | 7 cut(s) | 109, 110, 165, 202, 324, 974, 2745 |
| BmiI | GGNNCC | 9 cut(s) | 111, 167, 325, 366, 424, 759, 1109, 2089, 2115 |
| BmrFI | CCNGG | 8 cut(s) | 107, 108, 375, 427, 1475, 1578, 2489, 2657 |
| BmrI | ACTGGG | 3 cut(s) | 238, 1151, 1434 |
| BmuI | ACTGGG | 3 cut(s) | 238, 1151, 1434 |
| BpiI | GAAGAC | 3 cut(s) | 1775, 2225, 2351 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2687 |
| Bpu14I | TTCGAA | 2 cut(s) | 1337, 1791 |
| BpuEI | CTTGAG | 3 cut(s) | 554, 1139, 2609 |
| BpuMI | CCSGG | 4 cut(s) | 107, 108, 375, 427 |
| Bsa29I | ATCGAT | 1 cut(s) | 2447 |
| BsaAI | YACGTR | 3 cut(s) | 1346, 1397, 2780 |
| BsaHI | GRCGYC | 1 cut(s) | 2035 |
| BsaI | GGTCTC | 3 cut(s) | 1333, 2497, 2793 |
| BsaJI | CCNNGG | 3 cut(s) | 106, 977, 2238 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1927, 1957 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 162, 229, 319, 374, 419, 426, 1324, 1426, 2097 |
| Bse118I | RCCGGY | 1 cut(s) | 2632 |
| Bse1I | ACTGG | 9 cut(s) | 167, 233, 675, 876, 1146, 1429, 2064, 2538, 2553 |
| Bse3DI | GCAATG | 3 cut(s) | 19, 1032, 2559 |
| BseBI | CCWGG | 4 cut(s) | 1475, 1578, 2489, 2657 |
| BseCI | ATCGAT | 1 cut(s) | 2447 |
| BseDI | CCNNGG | 3 cut(s) | 106, 977, 2238 |
| BseGI | GGATG | 6 cut(s) | 299, 796, 1133, 1783, 1909, 2106 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 162, 229, 319, 374, 419, 426, 1324, 1426, 2097 |
| BseMI | GCAATG | 3 cut(s) | 19, 1032, 2559 |
| BseMII | CTCAG | 6 cut(s) | 333, 585, 1344, 1394, 1737, 1784 |
| BseNI | ACTGG | 9 cut(s) | 167, 233, 675, 876, 1146, 1429, 2064, 2538, 2553 |
| BseRI | GAGGAG | 2 cut(s) | 2606, 2621 |
| BseSI | GKGCMC | 2 cut(s) | 113, 369 |
| BseXI | GCAGC | 7 cut(s) | 67, 288, 322, 325, 1171, 1831, 2440 |
| BseYI | CCCAGC | 3 cut(s) | 121, 312, 369 |
| Bsh1236I | CGCG | 4 cut(s) | 185, 210, 2167, 2583 |
| Bsh1285I | CGRYCG | 1 cut(s) | 178 |
| BshFI | GGCC | 8 cut(s) | 111, 166, 326, 674, 976, 1768, 1974, 2747 |
| BshNI | GGYRCC | 2 cut(s) | 364, 422 |
| BshVI | ATCGAT | 1 cut(s) | 2447 |
| BsiEI | CGRYCG | 1 cut(s) | 178 |
| BsiHKAI | GWGCWC | 7 cut(s) | 252, 419, 1402, 1570, 1618, 1803, 2667 |
| BsiHKCI | CYCGRG | 5 cut(s) | 106, 412, 432, 2009, 2308 |
| BsiSI | CCGG | 6 cut(s) | 41, 107, 375, 420, 427, 2633 |
| BslFI | GGGAC | 1 cut(s) | 1461 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 162, 229, 319, 374, 419, 426, 1324, 1426, 2097 |
| BsmAI | GTCTC | 6 cut(s) | 997, 1212, 1333, 2237, 2497, 2793 |
| BsmFI | GGGAC | 1 cut(s) | 1461 |
| BsmI | GAATGC | 3 cut(s) | 1747, 2072, 2299 |
| BsnI | GGCC | 8 cut(s) | 111, 166, 326, 674, 976, 1768, 1974, 2747 |
| Bso31I | GGTCTC | 3 cut(s) | 1333, 2497, 2793 |
| BsoBI | CYCGRG | 5 cut(s) | 106, 412, 432, 2009, 2308 |
| Bsp119I | TTCGAA | 2 cut(s) | 1337, 1791 |
| Bsp120I | GGGCCC | 1 cut(s) | 109 |
| Bsp1286I | GDGCHC | 9 cut(s) | 113, 252, 369, 419, 1402, 1570, 1618, 1803, 2667 |
| Bsp1407I | TGTACA | 1 cut(s) | 690 |
| Bsp143I | GATC | 4 cut(s) | 7, 175, 1381, 2312 |
| Bsp68I | TCGCGA | 1 cut(s) | 2167 |
| BspANI | GGCC | 8 cut(s) | 111, 166, 326, 674, 976, 1768, 1974, 2747 |
| BspCNI | CTCAG | 6 cut(s) | 334, 586, 1343, 1395, 1738, 1783 |
| BspDI | ATCGAT | 1 cut(s) | 2447 |
| BspFNI | CGCG | 4 cut(s) | 185, 210, 2167, 2583 |
| BspLI | GGNNCC | 9 cut(s) | 111, 167, 325, 366, 424, 759, 1109, 2089, 2115 |
| BspMAI | CTGCAG | 2 cut(s) | 1627, 1849 |
| BspPI | GGATC | 1 cut(s) | 2307 |
| BspQI | GCTCTTC | 1 cut(s) | 2655 |
| BspT104I | TTCGAA | 2 cut(s) | 1337, 1791 |
| BspT107I | GGYRCC | 2 cut(s) | 364, 422 |
| BspTNI | GGTCTC | 3 cut(s) | 1333, 2497, 2793 |
| BsrBI | CCGCTC | 1 cut(s) | 2743 |
| BsrDI | GCAATG | 3 cut(s) | 19, 1032, 2559 |
| BsrFI | RCCGGY | 1 cut(s) | 2632 |
| BsrGI | TGTACA | 1 cut(s) | 690 |
| BsrI | ACTGG | 9 cut(s) | 167, 233, 675, 876, 1146, 1429, 2064, 2538, 2553 |
| BssAI | RCCGGY | 1 cut(s) | 2632 |
| BssECI | CCNNGG | 3 cut(s) | 106, 977, 2238 |
| BssMI | GATC | 4 cut(s) | 7, 175, 1381, 2312 |
| BssNI | GRCGYC | 1 cut(s) | 2035 |
| BssT1I | CCWWGG | 2 cut(s) | 977, 2238 |
| Bst2UI | CCWGG | 4 cut(s) | 1475, 1578, 2489, 2657 |
| Bst4CI | ACNGT | 7 cut(s) | 117, 224, 591, 1525, 1563, 1990, 2349 |
| Bst6I | CTCTTC | 5 cut(s) | 12, 764, 1082, 2331, 2655 |
| BstACI | GRCGYC | 1 cut(s) | 2035 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 881 |
| BstAUI | TGTACA | 1 cut(s) | 690 |
| BstBAI | YACGTR | 3 cut(s) | 1346, 1397, 2780 |
| BstBI | TTCGAA | 2 cut(s) | 1337, 1791 |
| BstC8I | GCNNGC | 7 cut(s) | 318, 1013, 1164, 1972, 2033, 2433, 2745 |
| BstDEI | CTNAG | 9 cut(s) | 342, 594, 1076, 1330, 1403, 1415, 1746, 1770, 2687 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1322 |
| BstF5I | GGATG | 6 cut(s) | 299, 796, 1133, 1783, 1909, 2106 |
| BstFNI | CGCG | 4 cut(s) | 185, 210, 2167, 2583 |
| BstHHI | GCGC | 1 cut(s) | 1588 |
| BstKTI | GATC | 4 cut(s) | 10, 178, 1384, 2315 |
| BstMAI | GTCTC | 6 cut(s) | 997, 1212, 1333, 2237, 2497, 2793 |
| BstMBI | GATC | 4 cut(s) | 7, 175, 1381, 2312 |
| BstMCI | CGRYCG | 1 cut(s) | 178 |
| BstNI | CCWGG | 4 cut(s) | 1475, 1578, 2489, 2657 |
| BstNSI | RCATGY | 2 cut(s) | 1015, 1454 |
| BstSCI | CCNGG | 8 cut(s) | 105, 106, 373, 425, 1473, 1576, 2487, 2655 |
| BstSFI | CTRYAG | 4 cut(s) | 1167, 1571, 1623, 1845 |
| BstSLI | GKGCMC | 2 cut(s) | 113, 369 |
| BstSNI | TACGTA | 1 cut(s) | 2780 |
| BstUI | CGCG | 4 cut(s) | 185, 210, 2167, 2583 |
| BstV1I | GCAGC | 7 cut(s) | 67, 288, 322, 325, 1171, 1831, 2440 |
| BstV2I | GAAGAC | 3 cut(s) | 1775, 2225, 2351 |
| BstX2I | RGATCY | 1 cut(s) | 2312 |
| BstYI | RGATCY | 1 cut(s) | 2312 |
| Bsu15I | ATCGAT | 1 cut(s) | 2447 |
| BsuRI | GGCC | 8 cut(s) | 111, 166, 326, 674, 976, 1768, 1974, 2747 |
| BsuTUI | ATCGAT | 1 cut(s) | 2447 |
| BtgZI | GCGATG | 1 cut(s) | 289 |
| BtrI | CACGTC | 1 cut(s) | 380 |
| BtsCI | GGATG | 6 cut(s) | 299, 796, 1133, 1783, 1909, 2106 |
| BtsIMutI | CAGTG | 9 cut(s) | 113, 587, 668, 1422, 1568, 1986, 2515, 2546, 2762 |
| BtuMI | TCGCGA | 1 cut(s) | 2167 |
| Cac8I | GCNNGC | 7 cut(s) | 318, 1013, 1164, 1972, 2033, 2433, 2745 |
| CfoI | GCGC | 1 cut(s) | 1588 |
| Cfr10I | RCCGGY | 1 cut(s) | 2632 |
| Cfr13I | GGNCC | 7 cut(s) | 109, 110, 165, 202, 324, 974, 2745 |
| Cfr9I | CCCGGG | 1 cut(s) | 106 |
| ClaI | ATCGAT | 1 cut(s) | 2447 |
| CseI | GACGC | 4 cut(s) | 141, 174, 2024, 2345 |
| CsiI | ACCWGGT | 1 cut(s) | 2487 |
| Csp6I | GTAC | 5 cut(s) | 494, 564, 691, 1205, 2777 |
| CviAII | CATG | 8 cut(s) | 676, 924, 953, 1012, 1267, 1451, 1535, 1694 |
| CviQI | GTAC | 5 cut(s) | 494, 564, 691, 1205, 2777 |
| DdeI | CTNAG | 9 cut(s) | 342, 594, 1076, 1330, 1403, 1415, 1746, 1770, 2687 |
| DpnI | GATC | 4 cut(s) | 9, 177, 1383, 2314 |
| DpnII | GATC | 4 cut(s) | 7, 175, 1381, 2312 |
| DraI | TTTAAA | 1 cut(s) | 1869 |
| EaeI | YGGCCR | 1 cut(s) | 672 |
| Eam1104I | CTCTTC | 5 cut(s) | 12, 764, 1082, 2331, 2655 |
| EarI | CTCTTC | 5 cut(s) | 12, 764, 1082, 2331, 2655 |
| EciI | GGCGGA | 1 cut(s) | 215 |
| Ecl136II | GAGCTC | 2 cut(s) | 417, 2665 |
| Eco105I | TACGTA | 1 cut(s) | 2780 |
| Eco130I | CCWWGG | 2 cut(s) | 977, 2238 |
| Eco24I | GRGCYC | 3 cut(s) | 113, 419, 2667 |
| Eco31I | GGTCTC | 3 cut(s) | 1333, 2497, 2793 |
| Eco32I | GATATC | 1 cut(s) | 2723 |
| Eco47I | GGWCC | 1 cut(s) | 202 |
| Eco53kI | GAGCTC | 2 cut(s) | 417, 2665 |
| Eco57I | CTGAAG | 6 cut(s) | 80, 927, 1659, 1944, 2421, 2583 |
| Eco72I | CACGTG | 2 cut(s) | 1346, 1397 |
| Eco88I | CYCGRG | 5 cut(s) | 106, 412, 432, 2009, 2308 |
| EcoICRI | GAGCTC | 2 cut(s) | 417, 2665 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1322 |
| EcoRII | CCWGG | 4 cut(s) | 1473, 1576, 2487, 2655 |
| EcoRV | GATATC | 1 cut(s) | 2723 |
| EcoT14I | CCWWGG | 2 cut(s) | 977, 2238 |
| EcoT22I | ATGCAT | 1 cut(s) | 301 |
| EcoT38I | GRGCYC | 3 cut(s) | 113, 419, 2667 |
| ErhI | CCWWGG | 2 cut(s) | 977, 2238 |
| FaeI | CATG | 8 cut(s) | 679, 927, 956, 1015, 1270, 1454, 1538, 1697 |
| FaqI | GGGAC | 1 cut(s) | 1461 |
| FatI | CATG | 8 cut(s) | 675, 923, 952, 1011, 1266, 1450, 1534, 1693 |
| FauI | CCCGC | 4 cut(s) | 177, 313, 2697, 2736 |
| FauNDI | CATATG | 1 cut(s) | 814 |
| FblI | GTMKAC | 1 cut(s) | 188 |
| FokI | GGATG | 6 cut(s) | 286, 803, 1140, 1790, 1916, 2113 |
| FriOI | GRGCYC | 3 cut(s) | 113, 419, 2667 |
| FspBI | CTAG | 6 cut(s) | 2066, 2084, 2225, 2282, 2475, 2481 |
| FspI | TGCGCA | 1 cut(s) | 1587 |
| GlaI | GCGC | 1 cut(s) | 1587 |
| GsaI | CCCAGC | 3 cut(s) | 125, 316, 373 |
| HaeIII | GGCC | 8 cut(s) | 111, 166, 326, 674, 976, 1768, 1974, 2747 |
| HapII | CCGG | 6 cut(s) | 41, 107, 375, 420, 427, 2633 |
| HgaI | GACGC | 4 cut(s) | 141, 174, 2024, 2345 |
| HhaI | GCGC | 1 cut(s) | 1588 |
| Hin1I | GRCGYC | 1 cut(s) | 2035 |
| Hin1II | CATG | 8 cut(s) | 679, 927, 956, 1015, 1270, 1454, 1538, 1697 |
| Hin6I | GCGC | 1 cut(s) | 1586 |
| HinP1I | GCGC | 1 cut(s) | 1586 |
| HincII | GTYRAC | 4 cut(s) | 189, 405, 1069, 2382 |
| HindII | GTYRAC | 4 cut(s) | 189, 405, 1069, 2382 |
| HindIII | AAGCTT | 3 cut(s) | 909, 1496, 2433 |
| HpaII | CCGG | 6 cut(s) | 41, 107, 375, 420, 427, 2633 |
| HphI | GGTGA | 3 cut(s) | 1441, 1504, 1654 |
| Hpy166II | GTNNAC | 7 cut(s) | 189, 405, 869, 1069, 2153, 2191, 2382 |
| Hpy8I | GTNNAC | 7 cut(s) | 189, 405, 869, 1069, 2153, 2191, 2382 |
| Hpy99I | CGWCG | 5 cut(s) | 28, 89, 135, 190, 2040 |
| HpyAV | CCTTC | 9 cut(s) | 159, 273, 399, 456, 1057, 1318, 1333, 1892, 2192 |
| HpyCH4III | ACNGT | 7 cut(s) | 117, 224, 591, 1525, 1563, 1990, 2349 |
| HpyCH4IV | ACGT | 4 cut(s) | 379, 1345, 1396, 2779 |
| HpyF3I | CTNAG | 9 cut(s) | 342, 594, 1076, 1330, 1403, 1415, 1746, 1770, 2687 |
| HpySE526I | ACGT | 4 cut(s) | 379, 1345, 1396, 2779 |
| Hsp92I | GRCGYC | 1 cut(s) | 2035 |
| Hsp92II | CATG | 8 cut(s) | 679, 927, 956, 1015, 1270, 1454, 1538, 1697 |
| HspAI | GCGC | 1 cut(s) | 1586 |
| Kzo9I | GATC | 4 cut(s) | 7, 175, 1381, 2312 |
| LguI | GCTCTTC | 1 cut(s) | 2655 |
| LmnI | GCTCC | 4 cut(s) | 255, 422, 1948, 2593 |
| Lsp1109I | GCAGC | 7 cut(s) | 67, 288, 322, 325, 1171, 1831, 2440 |
| MabI | ACCWGGT | 1 cut(s) | 2487 |
| MaeI | CTAG | 6 cut(s) | 2066, 2084, 2225, 2282, 2475, 2481 |
| MaeII | ACGT | 4 cut(s) | 379, 1345, 1396, 2779 |
| MaeIII | GTNAC | 3 cut(s) | 224, 1469, 2039 |
| MalI | GATC | 4 cut(s) | 9, 177, 1383, 2314 |
| MbiI | CCGCTC | 1 cut(s) | 2743 |
| MboI | GATC | 4 cut(s) | 7, 175, 1381, 2312 |
| MflI | RGATCY | 1 cut(s) | 2312 |
| MhlI | GDGCHC | 9 cut(s) | 113, 252, 369, 419, 1402, 1570, 1618, 1803, 2667 |
| MlsI | TGGCCA | 1 cut(s) | 674 |
| MluCI | AATT | 9 cut(s) | 354, 522, 609, 1256, 1821, 1864, 2262, 2451, 2463 |
| MluI | ACGCGT | 1 cut(s) | 183 |
| MluNI | TGGCCA | 1 cut(s) | 674 |
| MlyI | GAGTC | 7 cut(s) | 29, 400, 424, 1064, 2239, 2282, 2300 |
| MmeI | TCCRAC | 2 cut(s) | 629, 1755 |
| Mox20I | TGGCCA | 1 cut(s) | 674 |
| Mph1103I | ATGCAT | 1 cut(s) | 301 |
| MscI | TGGCCA | 1 cut(s) | 674 |
| MseI | TTAA | 4 cut(s) | 357, 1553, 1868, 2261 |
| MslI | CAYNNNNRTG | 3 cut(s) | 1271, 1427, 2796 |
| Msp20I | TGGCCA | 1 cut(s) | 674 |
| MspA1I | CMGCKG | 1 cut(s) | 58 |
| MspI | CCGG | 6 cut(s) | 41, 107, 375, 420, 427, 2633 |
| MspR9I | CCNGG | 8 cut(s) | 107, 108, 375, 427, 1475, 1578, 2489, 2657 |
| Mva1269I | GAATGC | 3 cut(s) | 1747, 2072, 2299 |
| MvaI | CCWGG | 4 cut(s) | 1475, 1578, 2489, 2657 |
| MvnI | CGCG | 4 cut(s) | 185, 210, 2167, 2583 |
| NciI | CCSGG | 4 cut(s) | 107, 108, 375, 427 |
| NdeI | CATATG | 1 cut(s) | 814 |
| NdeII | GATC | 4 cut(s) | 7, 175, 1381, 2312 |
| NlaIII | CATG | 8 cut(s) | 679, 927, 956, 1015, 1270, 1454, 1538, 1697 |
| NlaIV | GGNNCC | 9 cut(s) | 111, 167, 325, 366, 424, 759, 1109, 2089, 2115 |
| NmuCI | GTSAC | 3 cut(s) | 224, 1469, 2039 |
| NruI | TCGCGA | 1 cut(s) | 2167 |
| NsbI | TGCGCA | 1 cut(s) | 1587 |
| NsiI | ATGCAT | 1 cut(s) | 301 |
| NspI | RCATGY | 2 cut(s) | 1015, 1454 |
| NspV | TTCGAA | 2 cut(s) | 1337, 1791 |
| OliI | CACNNNNGTG | 1 cut(s) | 1427 |
| PaeI | GCATGC | 1 cut(s) | 1015 |
| PaeR7I | CTCGAG | 2 cut(s) | 432, 2308 |
| PciI | ACATGT | 1 cut(s) | 1450 |
| PciSI | GCTCTTC | 1 cut(s) | 2655 |
| PctI | GAATGC | 3 cut(s) | 1747, 2072, 2299 |
| PfeI | GAWTC | 9 cut(s) | 259, 601, 941, 1833, 2252, 2276, 2416, 2471, 2505 |
| PflFI | GACNNNGTC | 1 cut(s) | 230 |
| Ple19I | CGATCG | 1 cut(s) | 178 |
| PleI | GAGTC | 7 cut(s) | 28, 400, 424, 1064, 2238, 2282, 2300 |
| PmaCI | CACGTG | 2 cut(s) | 1346, 1397 |
| PmlI | CACGTG | 2 cut(s) | 1346, 1397 |
| PpsI | GAGTC | 7 cut(s) | 28, 400, 424, 1064, 2238, 2282, 2300 |
| Ppu21I | YACGTR | 3 cut(s) | 1346, 1397, 2780 |
| PscI | ACATGT | 1 cut(s) | 1450 |
| PshBI | ATTAAT | 1 cut(s) | 2261 |
| PsiI | TTATAA | 1 cut(s) | 2456 |
| Psp124BI | GAGCTC | 2 cut(s) | 419, 2667 |
| Psp6I | CCWGG | 4 cut(s) | 1473, 1576, 2487, 2655 |
| PspCI | CACGTG | 2 cut(s) | 1346, 1397 |
| PspFI | CCCAGC | 3 cut(s) | 121, 312, 369 |
| PspGI | CCWGG | 4 cut(s) | 1473, 1576, 2487, 2655 |
| PspN4I | GGNNCC | 9 cut(s) | 111, 167, 325, 366, 424, 759, 1109, 2089, 2115 |
| PspOMI | GGGCCC | 1 cut(s) | 109 |
| PspPI | GGNCC | 7 cut(s) | 109, 110, 165, 202, 324, 974, 2745 |
| PstI | CTGCAG | 2 cut(s) | 1627, 1849 |
| PsuI | RGATCY | 1 cut(s) | 2312 |
| PsyI | GACNNNGTC | 1 cut(s) | 230 |
| PvuI | CGATCG | 1 cut(s) | 178 |
| PvuII | CAGCTG | 1 cut(s) | 58 |
| RruI | TCGCGA | 1 cut(s) | 2167 |
| RsaI | GTAC | 5 cut(s) | 495, 565, 692, 1206, 2778 |
| RsaNI | GTAC | 5 cut(s) | 494, 564, 691, 1205, 2777 |
| RseI | CAYNNNNRTG | 3 cut(s) | 1271, 1427, 2796 |
| SacI | GAGCTC | 2 cut(s) | 419, 2667 |
| SalI | GTCGAC | 1 cut(s) | 187 |
| SapI | GCTCTTC | 1 cut(s) | 2655 |
| SaqAI | TTAA | 4 cut(s) | 357, 1553, 1868, 2261 |
| Sau3AI | GATC | 4 cut(s) | 7, 175, 1381, 2312 |
| Sau96I | GGNCC | 7 cut(s) | 109, 110, 165, 202, 324, 974, 2745 |
| SchI | GAGTC | 7 cut(s) | 29, 400, 424, 1064, 2239, 2282, 2300 |
| ScrFI | CCNGG | 8 cut(s) | 107, 108, 375, 427, 1475, 1578, 2489, 2657 |
| SduI | GDGCHC | 9 cut(s) | 113, 252, 369, 419, 1402, 1570, 1618, 1803, 2667 |
| SexAI | ACCWGGT | 1 cut(s) | 2487 |
| SfcI | CTRYAG | 4 cut(s) | 1167, 1571, 1623, 1845 |
| Sfr274I | CTCGAG | 2 cut(s) | 432, 2308 |
| SfuI | TTCGAA | 2 cut(s) | 1337, 1791 |
| SinI | GGWCC | 1 cut(s) | 202 |
| SlaI | CTCGAG | 2 cut(s) | 432, 2308 |
| SmaI | CCCGGG | 1 cut(s) | 108 |
| SmiI | ATTTAAAT | 1 cut(s) | 1869 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 1271, 1427, 2796 |
| SmlI | CTYRAG | 5 cut(s) | 432, 533, 1154, 2308, 2588 |
| SmoI | CTYRAG | 5 cut(s) | 432, 533, 1154, 2308, 2588 |
| SnaBI | TACGTA | 1 cut(s) | 2780 |
| SphI | GCATGC | 1 cut(s) | 1015 |
| Sse9I | AATT | 9 cut(s) | 354, 522, 609, 1256, 1821, 1864, 2262, 2451, 2463 |
| SspI | AATATT | 3 cut(s) | 577, 849, 1608 |
| SspMI | CTAG | 6 cut(s) | 2066, 2084, 2225, 2282, 2475, 2481 |
| SstI | GAGCTC | 2 cut(s) | 419, 2667 |
| StyD4I | CCNGG | 8 cut(s) | 105, 106, 373, 425, 1473, 1576, 2487, 2655 |
| StyI | CCWWGG | 2 cut(s) | 977, 2238 |
| SwaI | ATTTAAAT | 1 cut(s) | 1869 |
| TaaI | ACNGT | 7 cut(s) | 117, 224, 591, 1525, 1563, 1990, 2349 |
| TaiI | ACGT | 4 cut(s) | 382, 1348, 1399, 2782 |
| TaqI | TCGA | 8 cut(s) | 26, 130, 188, 433, 1337, 1791, 2309, 2447 |
| TasI | AATT | 9 cut(s) | 354, 522, 609, 1256, 1821, 1864, 2262, 2451, 2463 |
| TatI | WGTACW | 2 cut(s) | 690, 1204 |
| TauI | GCSGC | 3 cut(s) | 84, 200, 517 |
| TfiI | GAWTC | 9 cut(s) | 259, 601, 941, 1833, 2252, 2276, 2416, 2471, 2505 |
| Tru1I | TTAA | 4 cut(s) | 357, 1553, 1868, 2261 |
| Tru9I | TTAA | 4 cut(s) | 357, 1553, 1868, 2261 |
| TscAI | CASTG | 9 cut(s) | 120, 594, 675, 1429, 1568, 1993, 2515, 2553, 2769 |
| TseFI | GTSAC | 3 cut(s) | 224, 1469, 2039 |
| TseI | GCWGC | 7 cut(s) | 55, 276, 335, 338, 1159, 1844, 2428 |
| Tsp45I | GTSAC | 3 cut(s) | 224, 1469, 2039 |
| TspGWI | ACGGA | 2 cut(s) | 85, 228 |
| TspMI | CCCGGG | 1 cut(s) | 106 |
| TspRI | CASTG | 9 cut(s) | 120, 594, 675, 1429, 1568, 1993, 2515, 2553, 2769 |
| Tth111I | GACNNNGTC | 1 cut(s) | 230 |
| VpaK11BI | GGWCC | 1 cut(s) | 202 |
| VspI | ATTAAT | 1 cut(s) | 2261 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1322 |
| XapI | RAATTY | 3 cut(s) | 1256, 1864, 2463 |
| XbaI | TCTAGA | 2 cut(s) | 2065, 2224 |
| XceI | RCATGY | 2 cut(s) | 1015, 1454 |
| XhoI | CTCGAG | 2 cut(s) | 432, 2308 |
| XmaI | CCCGGG | 1 cut(s) | 106 |
| XmiI | GTMKAC | 1 cut(s) | 188 |
| XspI | CTAG | 6 cut(s) | 2066, 2084, 2225, 2282, 2475, 2481 |
| Zsp2I | ATGCAT | 1 cut(s) | 301 |