Rw4G010760

Ion channel CASTOR-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001000
Physical Location & Seq
Reverse (-)
25159094 .. 25159904
811 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G010760.1

Sequence Viewer

Length: 696 bp
ATGGTCTCCCTCATGTTTTTATCAATCCCTCTTGTGGTTTTCAAGTACATTGACCATGTCTCCAGATCACGGTCACCGGAGAACATCTCTGAAGAAGAATCTCTAAACAAACAGCTAGCGTACCGTGTCGATTTGTTTTTATCAGTCCACCCGTATTCCAAGCCACTGACATTGTTGATTGCAACACTGTTACTTATTTGTCTTGGGGGGTTGGCATTGTTTGGAGTGAAAGATGATAGCTTGGCGGAATGTCTTTGGTTATCGTGGACGTATGTGGCTGATTCTGGCAACCATACTGACTCTGAAAAAATTGGCGAAAGGCTTGTTTCAGTTTCCATTAGCTTTGGTGGGATGCTCATATTTGCAATGATGCTTGGACTTGTTTCTGATGCAATTTCGGAGAAGTTTGACTCATTGAGGAAAGGAAGAAGTGAGGTAGTGGAGCAAAACCATACTTTAATTCTCGGTTGGAGCGATAAATTGGGATCATTATTGAACCAGATTGCAATAGCTAATGAGAGTTTGGGTGGAGGGATTGTTGTAGTTATGGCTGAGCGAGACAAAGAGGAAATGGAACTTGACATTGGTAAAATGGAGTTTGATTTCAAAGGAACATCTGTAATATGCAGAAGTGGGAGCCCTCTTATTCTTGCTGACCTGAAAAAGGTAGGACAGTTTGCTTTCTTGTTACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Castor_Poll_mid

Protein Analysis

231

Amino Acids

25.45

Weight (kDa)

5.05

Isoelectric Point (pI)

42.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 245
AclWI GGATC 1 cut(s) 493
AcuI CTGAAG 1 cut(s) 111
AfaI GTAC 2 cut(s) 47, 122
AfiI CCNNNNNNNGG 3 cut(s) 34, 69, 664
AgsI TTSAA 3 cut(s) 43, 496, 607
AjuI GAANNNNNNNTTGG 2 cut(s) 567, 599
AluBI AGCT 4 cut(s) 115, 240, 342, 512
AluI AGCT 4 cut(s) 115, 240, 342, 512
Alw26I GTCTC 3 cut(s) 10, 64, 552
AlwI GGATC 1 cut(s) 493
AsuHPI GGTGA 1 cut(s) 66
AsuNHI GCTAGC 1 cut(s) 115
BanII GRGCYC 1 cut(s) 641
BcoDI GTCTC 3 cut(s) 10, 64, 552
BfaI CTAG 1 cut(s) 116
BlpI GCTNAGC 1 cut(s) 552
BmiI GGNNCC 1 cut(s) 638
BmsI GCATC 3 cut(s) 342, 360, 379
BmtI GCTAGC 1 cut(s) 119
BplI GAGNNNNNCTC 2 cut(s) 71, 103
BpmI CTGGAG 1 cut(s) 46
Bpu1102I GCTNAGC 1 cut(s) 552
BsaI GGTCTC 1 cut(s) 10
BsaWI WCCGGW 1 cut(s) 76
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 522, 552
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 69, 664
Bse3DI GCAATG 1 cut(s) 372
BseGI GGATG 1 cut(s) 357
BseLI CCNNNNNNNGG 3 cut(s) 34, 69, 664
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 1 cut(s) 543
BsiSI CCGG 1 cut(s) 77
BslI CCNNNNNNNGG 3 cut(s) 34, 69, 664
BsmAI GTCTC 3 cut(s) 10, 64, 552
Bso31I GGTCTC 1 cut(s) 10
Bsp1286I GDGCHC 1 cut(s) 641
Bsp143I GATC 2 cut(s) 65, 485
Bsp1720I GCTNAGC 1 cut(s) 552
BspACI CCGC 1 cut(s) 245
BspCNI CTCAG 1 cut(s) 544
BspLI GGNNCC 1 cut(s) 638
BspOI GCTAGC 1 cut(s) 119
BspPI GGATC 1 cut(s) 493
BspTNI GGTCTC 1 cut(s) 10
BsrDI GCAATG 1 cut(s) 372
BssMI GATC 2 cut(s) 65, 485
Bst4CI ACNGT 4 cut(s) 72, 125, 189, 675
BstC8I GCNNGC 1 cut(s) 117
BstDEI CTNAG 1 cut(s) 552
BstEII GGTNACC 1 cut(s) 72
BstENI CCTNNNNNAGG 1 cut(s) 662
BstF5I GGATG 1 cut(s) 357
BstKTI GATC 2 cut(s) 68, 488
BstMAI GTCTC 3 cut(s) 10, 64, 552
BstMBI GATC 2 cut(s) 65, 485
BstPI GGTNACC 1 cut(s) 72
BtsCI GGATG 1 cut(s) 357
BtsIMutI CAGTG 2 cut(s) 164, 185
Cac8I GCNNGC 1 cut(s) 117
Csp6I GTAC 2 cut(s) 46, 121
CspCI CAANNNNNGTGG 2 cut(s) 153, 188
CviAII CATG 2 cut(s) 13, 56
CviJI RGCY 9 cut(s) 115, 163, 240, 278, 322, 342, 512, 551, 639
CviKI_1 RGCY 9 cut(s) 115, 163, 240, 278, 322, 342, 512, 551, 639
CviQI GTAC 2 cut(s) 46, 121
DdeI CTNAG 1 cut(s) 552
DpnI GATC 2 cut(s) 67, 487
DpnII GATC 2 cut(s) 65, 485
EciI GGCGGA 1 cut(s) 260
Eco24I GRGCYC 1 cut(s) 641
Eco31I GGTCTC 1 cut(s) 10
Eco57I CTGAAG 1 cut(s) 111
Eco91I GGTNACC 1 cut(s) 72
EcoNI CCTNNNNNAGG 1 cut(s) 662
EcoO65I GGTNACC 1 cut(s) 72
EcoT38I GRGCYC 1 cut(s) 641
FaeI CATG 2 cut(s) 16, 59
FaiI YATR 8 cut(s) 14, 57, 273, 294, 359, 453, 548, 625
FatI CATG 2 cut(s) 12, 55
FokI GGATG 1 cut(s) 364
FriOI GRGCYC 1 cut(s) 641
FspBI CTAG 1 cut(s) 116
GsuI CTGGAG 1 cut(s) 46
HapII CCGG 1 cut(s) 77
Hin1II CATG 2 cut(s) 16, 59
HinfI GANTC 4 cut(s) 98, 281, 299, 410
HpaII CCGG 1 cut(s) 77
HphI GGTGA 1 cut(s) 66
Hpy166II GTNNAC 2 cut(s) 148, 267
Hpy188I TCNGA 4 cut(s) 91, 304, 388, 400
Hpy188III TCNNGA 1 cut(s) 63
Hpy8I GTNNAC 2 cut(s) 148, 267
HpyCH4III ACNGT 4 cut(s) 72, 125, 189, 675
HpyCH4IV ACGT 1 cut(s) 269
HpyCH4V TGCA 5 cut(s) 182, 365, 392, 506, 627
HpyF3I CTNAG 1 cut(s) 552
HpySE526I ACGT 1 cut(s) 269
Hsp92II CATG 2 cut(s) 16, 59
Kzo9I GATC 2 cut(s) 65, 485
LmnI GCTCC 3 cut(s) 442, 471, 636
LpnPI CCDG 5 cut(s) 76, 90, 270, 512, 671
LweI GCATC 3 cut(s) 342, 360, 379
MaeI CTAG 1 cut(s) 116
MaeII ACGT 1 cut(s) 269
MaeIII GTNAC 3 cut(s) 72, 189, 687
MalI GATC 2 cut(s) 67, 487
MboI GATC 2 cut(s) 65, 485
MboII GAAGA 3 cut(s) 104, 107, 438
MhlI GDGCHC 1 cut(s) 641
MluCI AATT 4 cut(s) 309, 393, 459, 479
MlyI GAGTC 2 cut(s) 293, 404
MmeI TCCRAC 1 cut(s) 449
MnlI CCTC 7 cut(s) 20, 39, 411, 427, 524, 559, 651
MseI TTAA 2 cut(s) 458, 694
MspI CCGG 1 cut(s) 77
NdeII GATC 2 cut(s) 65, 485
NheI GCTAGC 1 cut(s) 115
NlaIII CATG 2 cut(s) 16, 59
NlaIV GGNNCC 1 cut(s) 638
NmuCI GTSAC 1 cut(s) 72
PcsI WCGNNNNNNNCGW 1 cut(s) 471
PfeI GAWTC 2 cut(s) 98, 281
PflFI GACNNNGTC 1 cut(s) 56
PleI GAGTC 2 cut(s) 293, 404
PpsI GAGTC 2 cut(s) 293, 404
PspEI GGTNACC 1 cut(s) 72
PspN4I GGNNCC 1 cut(s) 638
PsyI GACNNNGTC 1 cut(s) 56
RsaI GTAC 2 cut(s) 47, 122
RsaNI GTAC 2 cut(s) 46, 121
SaqAI TTAA 2 cut(s) 458, 694
Sau3AI GATC 2 cut(s) 65, 485
SchI GAGTC 2 cut(s) 293, 404
SduI GDGCHC 1 cut(s) 641
SetI ASST 8 cut(s) 117, 242, 272, 344, 438, 514, 660, 669
SfaNI GCATC 3 cut(s) 342, 360, 379
Sse9I AATT 4 cut(s) 309, 393, 459, 479
SsiI CCGC 1 cut(s) 245
SspMI CTAG 1 cut(s) 116
TaaI ACNGT 4 cut(s) 72, 125, 189, 675
TaiI ACGT 1 cut(s) 272
TaqI TCGA 1 cut(s) 129
TasI AATT 4 cut(s) 309, 393, 459, 479
TatI WGTACW 1 cut(s) 45
TfiI GAWTC 2 cut(s) 98, 281
Tru1I TTAA 2 cut(s) 458, 694
Tru9I TTAA 2 cut(s) 458, 694
TscAI CASTG 2 cut(s) 171, 192
TseFI GTSAC 1 cut(s) 72
Tsp45I GTSAC 1 cut(s) 72
TspRI CASTG 2 cut(s) 171, 192
Tth111I GACNNNGTC 1 cut(s) 56
XagI CCTNNNNNAGG 1 cut(s) 662
XspI CTAG 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.