MD08G1240900.v1.1

Ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001000
Physical Location & Seq
Forward (+)
30675402 .. 30683781
8380 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1240900.v1.1.491

Sequence Viewer

Length: 2808 bp
ATGGTGGATGGCAATGATGACCCGTCGTCGAAGCCGAAAAAGTCGGAGAGCCCTCCGCAGCTCAAGAAATCACGAACCGTCATCACCACCACCGCATCCGACGACGCGGAGCCCACGACCCCAATCTTCCCGGGCCCACTCTTCCCCGCCGTCCGACGCGTCTCCACCCTCCCTCCCACCTCCCGCCAGTGGCCGCCGCCCGATCGCCCCGCCTCCTTCTCCTCCTCCTCCTCCTTCAATGCGCCCGCCGACCACCGAGACTTCTCCGACCGCGATTGGGTCTTCCCTTCCCTCGTTGTCCCTCAGAACACTTCCAAACGCGGCAAGAAGCTCCCCTCCTCCTCCTCCACTGATTCCCGTGCCGCGCATGCTAATAAGCTGGTGGACGCGCCGCAACGGATCGCCTCTCAGCCTCCTCCTGCGGCCTCGGTCCCGAAGCCGGAGGCTGAGAGGAAGAAATTGAAGGTGGTGCCGAATCTAACCTCTGCCTCTTCTGCTAGTGAGTCGACTCGGCCGAGTTCGAGGCGGACTGCGGAGTCCAAGCGTCCTTTGTTACTAGTTGTGCTCACTTTCACTTGTATATTATCTGTACCTTATGCAGTTTATCTCCGGCAGAGAGTTGCAGAACTTGAGGAATGGTGTAACCCTAAAAATATGGATTCAAATAGCAGCATTAAGGATTTTCTGGTGGAGAAGAATCCACCACTGTTTCATTTTAGTGCTGAAAGGAGAACTGTTGCTTTGTATACTGTGGTCTTTTCGATTACTATCCCATTTGTATTGTACAAACATCTTGATTCTCTTTCCCAACTAAGGAGTCTCTCAAAACGGATCAAGAATAACAACGAGGAGGTTCCCATAAAGAAGAGGATTGCATATATGGTTGATGTGTGTTTCTCCGTCTATCCATATGCCAAACTGCTTGCTCTTCTCTTTGCAACGATGTTTCTTATAGGGTTTGGTGGATTGGCATTATATGCAGTCAATACAAACAGCTTCGCTGAAGCTCTTTGGCTCTCATGGACTTTTGTTGCTGATTCTGGAAACCATGCTGACACAGAAGGCATCGGGCCAAGGATAGTTTCTGTCTCTATTAGTTCAGGAGGCATGCTAATATTTGCAATGATGCTTGGGCTTGTTTCTGATGCTATATCAGAGAAGGTTGATTCACTGCGGAAAGGGAAGAGTGAAGTCATTGAAAGGGACCACATCCTCATTCTTGGATGGAGTGACAAATTGGGTTCACTTCTAAAGCAGCTAGCAATAGCAAACAAGAGTGTTGGTGGTGGAGTTGTCGTTGTACTTGCTGAAAGAGACAAGGAGGAAATGGAGCTGGATATAGCAAAGCTTGAATTTGACTTCATGGGGACTTCGGTTATATGCAGAAGTGGCAGCCCTCTTATACTGGCCGACCTGAAGAAGGTCTCAGTTTCAAAAGCACGTGCTATCATTGTATTGGCATCTGATGAAAACGCAGATCAGAGTGATGCACGTGCTTTGAGGGTTGTGCTCAGCCTTACAGGGGTGAAAGAGGGCTTGAGGGGTCATGTTGTCGTAGAGATGAGTGACCTTGACAATGAGCCTCTGGTGAAGCTTGTTGGAGGAGAACTCATTGAAACAGTTGTAGCACATGATGTGATTGGACGGTTGATGATACAGTGTGCTCTGCAACCTGGTCTTGCACAGATATGGGAGGACATATTGGGTTTTGAGAATGCTGAGTTTTACATCAAAAGGTGGCCTCAACTGGATGGTCTTCGTTTTGAGAATGTGCTTATTTCATTTCCTGATGCAATTCCTTGTGGAATTAAGGTTGCTGCAGATGGTGGAAAGATAATCTTAAATCCGGATGATAATTATGTTATGAAAGAAGGGGATGAAGTTCTTGTTATAGCGGAAGATGATGACACTTATGCTCCTTGTCCCTTTCCAGAGGTTTGTGCAGGCTACTGTCCAAAGACAGTTGAACCTCCCAAGTACCCCGAGAAGATATTGTTTTGCGGCTGGCGTCGGGATATAGACGATATGATTATGGTTCTAGAGGCATTCCTGCCTCCGGGTTCAGAACTTTGGATGTTCAATGAAGTTCCTGAAAAAGAAAGAGATAAGAAACTTACAGATGGTGGACTGGATGTTTCTAGATTGGAGAACATTAAGCTTGTCCACAGAGAAGGAAATGCTGTGATCAAACGACATTTAGAGAATCTTCCTTTGGAGACTTTTGATTCTATATTAATTCTTGCAGATGAATCACTAGAGGACTCTGTTGTTCATTCTGACTCAAGATCACTTGCCACTCTTCTCCTCATACGAGATATACAGTCAAAGCGTCTCCCTGACAAAGATACAAAGCCAACTTCTTTGCGATTATCAGCATTTTCGCACAGCTCTTGGATCCGTGAAATGCAGCAAGCTTCAGACAAGTCGATCATAATTAGTGAAATTCTGGATTCTAGGACTAGAAACTTGGTCTCAGTTTCTAGAATCAGTGATTATGTGCTATCAAATGAACTGGTTAGTATGGCGCTGGCAATGGTGGCTGAAGACAAGCAAATTAATCGCGTCCTTGAGGAGCTATTTGCTGAAGAGGGAAATGAGATGTGCATTAAACCAGCAGAACTCTATTTATATGACCAGGAAGAACTCAGCTTTTACGATATTATGCTCAGGGGTCGTCAAAGACAGGAAGTTGTGATTGGATATCGTCTTGTAAACACAGAGCGAGCCATAATTAATCCAGCACCGAAGTCAGAGCGAAGGAAATGGTCCCATGATGATGTTTTCGTTGTTATCTCTGTAAGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Castor_Poll_mid

Protein Analysis

936

Amino Acids

103.95

Weight (kDa)

5.52

Isoelectric Point (pI)

49.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Slo-like_RCK PF22614 401 - 505 8.8e-07 Calcium-activated potassium channel slowpoke-like RCK domain
Castor_Poll_mid PF06241 544 - 641 1.8e-42 Castor and Pollux, part of voltage-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 535
AccB1I GGYRCC 1 cut(s) 469
AccI GTMKAC 2 cut(s) 506, 746
AccII CGCG 7 cut(s) 107, 159, 273, 321, 365, 389, 2562
AccIII TCCGGA 1 cut(s) 1846
AclWI GGATC 4 cut(s) 407, 839, 2389, 2402
AcoI YGGCCR 3 cut(s) 191, 512, 1407
AcsI RAATTY 2 cut(s) 1352, 2442
AcuI CTGAAG 5 cut(s) 1023, 1436, 2400, 2562, 2604
AcvI CACGTG 2 cut(s) 1442, 1493
AcyI GRCGYC 1 cut(s) 2008
AfaI GTAC 4 cut(s) 591, 785, 1302, 1979
AfiI CCNNNNNNNGG 7 cut(s) 189, 277, 439, 813, 1420, 1522, 2056
AflIII ACRYGT 1 cut(s) 157
AgsI TTSAA 9 cut(s) 238, 463, 663, 1199, 1352, 1434, 1616, 1967, 2080
AhdI GACNNNNNGTC 1 cut(s) 25
AhlI ACTAGT 1 cut(s) 556
AjnI CCWGG 2 cut(s) 1672, 2634
AjuI GAANNNNNNNTTGG 6 cut(s) 1066, 1098, 2195, 2227, 2679, 2711
Alw21I GWGCWC 3 cut(s) 567, 1512, 1666
Alw26I GTCTC 9 cut(s) 166, 252, 824, 1093, 1308, 1429, 2210, 2336, 2476
AlwI GGATC 4 cut(s) 407, 839, 2389, 2402
Ama87I CYCGRG 2 cut(s) 130, 1982
Aor13HI TCCGGA 1 cut(s) 1846
AoxI GGCC 7 cut(s) 133, 191, 423, 512, 1070, 1407, 1739
ApaI GGGCCC 1 cut(s) 137
ApeKI GCWGC 6 cut(s) 58, 669, 1255, 1392, 1817, 2407
ApoI RAATTY 2 cut(s) 1352, 2442
AseI ATTAAT 3 cut(s) 2234, 2556, 2733
AspLEI GCGC 4 cut(s) 244, 367, 391, 2527
AspS9I GGNCC 6 cut(s) 133, 134, 430, 1070, 1204, 2766
AsuC2I CCSGG 3 cut(s) 131, 132, 2058
AsuHPI GGTGA 3 cut(s) 76, 1537, 1600
AsuNHI GCTAGC 1 cut(s) 1258
AvaI CYCGRG 2 cut(s) 130, 1982
AvaII GGWCC 3 cut(s) 430, 1204, 2766
BaeGI GKGCMC 1 cut(s) 137
BamHI GGATCC 1 cut(s) 2394
BanI GGYRCC 1 cut(s) 469
BanII GRGCYC 3 cut(s) 53, 114, 137
BbrPI CACGTG 2 cut(s) 1442, 1493
BbsI GAAGAC 3 cut(s) 274, 1748, 2550
Bbv12I GWGCWC 3 cut(s) 567, 1512, 1666
BbvI GCAGC 6 cut(s) 70, 681, 1267, 1404, 1804, 2419
BccI CCATC 5 cut(s) 2, 1218, 1745, 1817, 2114
BceAI ACGGC 1 cut(s) 134
BcgI CGANNNNNNTGC 2 cut(s) 2540, 2574
BciT130I CCWGG 2 cut(s) 1674, 2636
BclI TGATCA 1 cut(s) 2184
BcnI CCSGG 3 cut(s) 131, 132, 2058
BcoDI GTCTC 9 cut(s) 166, 252, 824, 1093, 1308, 1429, 2210, 2336, 2476
BcuI ACTAGT 1 cut(s) 556
BfaI CTAG 9 cut(s) 498, 557, 1259, 2039, 2139, 2255, 2454, 2460, 2481
BfmI CTRYAG 1 cut(s) 1818
BfoI RGCGCY 1 cut(s) 2528
BlpI GCTNAGC 1 cut(s) 1511
Bme1390I CCNGG 5 cut(s) 131, 132, 1674, 2058, 2636
Bme18I GGWCC 3 cut(s) 430, 1204, 2766
BmeRI GACNNNNNGTC 1 cut(s) 25
BmeT110I CYCGRG 2 cut(s) 130, 1982
BmgT120I GGNCC 6 cut(s) 133, 134, 430, 1070, 1204, 2766
BmiI GGNNCC 8 cut(s) 111, 135, 432, 471, 855, 1205, 2396, 2768
BmrFI CCNGG 5 cut(s) 131, 132, 1674, 2058, 2636
BmsI GCATC 7 cut(s) 104, 1074, 1116, 1135, 1469, 1477, 1780
BmtI GCTAGC 1 cut(s) 1262
BpiI GAAGAC 3 cut(s) 274, 1748, 2550
BplI GAGNNNNNCTC 2 cut(s) 1593, 1625
Bpu10I CCTNAGC 1 cut(s) 2666
Bpu1102I GCTNAGC 1 cut(s) 1511
BpuEI CTTGAG 5 cut(s) 47, 650, 1558, 2266, 2588
BpuMI CCSGG 3 cut(s) 131, 132, 2058
BsaAI YACGTR 2 cut(s) 1442, 1493
BsaBI GATNNNNATC 1 cut(s) 767
BsaHI GRCGYC 1 cut(s) 2008
BsaI GGTCTC 2 cut(s) 1429, 2476
BsaJI CCNNGG 3 cut(s) 130, 426, 1073
BsaWI WCCGGW 1 cut(s) 1846
BsaXI ACNNNNNCTCC 6 cut(s) 157, 187, 1281, 1311, 1900, 1930
Bsc4I CCNNNNNNNGG 7 cut(s) 189, 277, 439, 813, 1420, 1522, 2056
Bse1I ACTGG 5 cut(s) 187, 1410, 1752, 2133, 2517
Bse3DI GCAATG 3 cut(s) 19, 1128, 2538
Bse8I GATNNNNATC 1 cut(s) 767
BseAI TCCGGA 1 cut(s) 1846
BseBI CCWGG 2 cut(s) 1674, 2636
BseDI CCNNGG 3 cut(s) 130, 426, 1073
BseGI GGATG 9 cut(s) 13, 95, 1209, 1229, 1756, 1855, 1882, 2079, 2137
BseJI GATNNNNATC 1 cut(s) 767
BseLI CCNNNNNNNGG 7 cut(s) 189, 277, 439, 813, 1420, 1522, 2056
BseMI GCAATG 3 cut(s) 19, 1128, 2538
BseMII CTCAG 9 cut(s) 317, 422, 438, 1440, 1525, 1710, 2487, 2659, 2680
BseNI ACTGG 5 cut(s) 187, 1410, 1752, 2133, 2517
BseSI GKGCMC 1 cut(s) 137
BseX3I CGGCCG 1 cut(s) 512
BseXI GCAGC 6 cut(s) 70, 681, 1267, 1404, 1804, 2419
BsgI GTGCAG 1 cut(s) 1962
Bsh1236I CGCG 7 cut(s) 107, 159, 273, 321, 365, 389, 2562
Bsh1285I CGRYCG 3 cut(s) 205, 271, 515
BshFI GGCC 7 cut(s) 135, 193, 425, 514, 1072, 1409, 1741
BshNI GGYRCC 1 cut(s) 469
BsiEI CGRYCG 3 cut(s) 205, 271, 515
BsiHKAI GWGCWC 3 cut(s) 567, 1512, 1666
BsiHKCI CYCGRG 2 cut(s) 130, 1982
BsiSI CCGG 5 cut(s) 131, 440, 610, 1847, 2057
BslFI GGGAC 6 cut(s) 284, 416, 1217, 1381, 1908, 2752
BslI CCNNNNNNNGG 7 cut(s) 189, 277, 439, 813, 1420, 1522, 2056
BsmAI GTCTC 9 cut(s) 166, 252, 824, 1093, 1308, 1429, 2210, 2336, 2476
BsmBI CGTCTC 2 cut(s) 166, 2336
BsmFI GGGAC 6 cut(s) 284, 416, 1217, 1381, 1908, 2752
BsmI GAATGC 2 cut(s) 1720, 2045
BsnI GGCC 7 cut(s) 135, 193, 425, 514, 1072, 1409, 1741
Bso31I GGTCTC 2 cut(s) 1429, 2476
BsoBI CYCGRG 2 cut(s) 130, 1982
Bsp120I GGGCCC 1 cut(s) 133
Bsp1286I GDGCHC 6 cut(s) 53, 114, 137, 567, 1512, 1666
Bsp13I TCCGGA 1 cut(s) 1846
Bsp1407I TGTACA 1 cut(s) 783
Bsp143I GATC 8 cut(s) 202, 399, 831, 1477, 2184, 2285, 2394, 2427
Bsp1720I GCTNAGC 1 cut(s) 1511
BspANI GGCC 7 cut(s) 135, 193, 425, 514, 1072, 1409, 1741
BspCNI CTCAG 9 cut(s) 316, 421, 439, 1439, 1524, 1711, 2486, 2658, 2679
BspEI TCCGGA 1 cut(s) 1846
BspFNI CGCG 7 cut(s) 107, 159, 273, 321, 365, 389, 2562
BspLI GGNNCC 8 cut(s) 111, 135, 432, 471, 855, 1205, 2396, 2768
BspMAI CTGCAG 1 cut(s) 1822
BspOI GCTAGC 1 cut(s) 1262
BspPI GGATC 4 cut(s) 407, 839, 2389, 2402
BspQI GCTCTTC 1 cut(s) 933
BspT107I GGYRCC 1 cut(s) 469
BspTNI GGTCTC 2 cut(s) 1429, 2476
BsrDI GCAATG 3 cut(s) 19, 1128, 2538
BsrGI TGTACA 1 cut(s) 783
BsrI ACTGG 5 cut(s) 187, 1410, 1752, 2133, 2517
BssECI CCNNGG 3 cut(s) 130, 426, 1073
BssMI GATC 8 cut(s) 202, 399, 831, 1477, 2184, 2285, 2394, 2427
BssNAI GTATAC 1 cut(s) 747
BssNI GRCGYC 1 cut(s) 2008
BssT1I CCWWGG 1 cut(s) 1073
Bst1107I GTATAC 1 cut(s) 747
Bst2UI CCWGG 2 cut(s) 1674, 2636
Bst6I CTCTTC 7 cut(s) 146, 496, 860, 933, 1178, 2304, 2580
BstACI GRCGYC 1 cut(s) 2008
BstAPI GCANNNNNTGC 1 cut(s) 977
BstAUI TGTACA 1 cut(s) 783
BstBAI YACGTR 2 cut(s) 1442, 1493
BstENI CCTNNNNNAGG 1 cut(s) 1418
BstF5I GGATG 9 cut(s) 13, 95, 1209, 1229, 1756, 1855, 1882, 2079, 2137
BstFNI CGCG 7 cut(s) 107, 159, 273, 321, 365, 389, 2562
BstH2I RGCGCY 1 cut(s) 2528
BstHHI GCGC 4 cut(s) 244, 367, 391, 2527
BstKTI GATC 8 cut(s) 205, 402, 834, 1480, 2187, 2288, 2397, 2430
BstMAI GTCTC 9 cut(s) 166, 252, 824, 1093, 1308, 1429, 2210, 2336, 2476
BstMBI GATC 8 cut(s) 202, 399, 831, 1477, 2184, 2285, 2394, 2427
BstMCI CGRYCG 3 cut(s) 205, 271, 515
BstMWI GCNNNNNNNGC 5 cut(s) 368, 494, 977, 1389, 2537
BstNI CCWGG 2 cut(s) 1674, 2636
BstNSI RCATGY 2 cut(s) 371, 1111
BstSCI CCNGG 5 cut(s) 129, 130, 1672, 2056, 2634
BstSFI CTRYAG 1 cut(s) 1818
BstSLI GKGCMC 1 cut(s) 137
BstUI CGCG 7 cut(s) 107, 159, 273, 321, 365, 389, 2562
BstV1I GCAGC 6 cut(s) 70, 681, 1267, 1404, 1804, 2419
BstV2I GAAGAC 3 cut(s) 274, 1748, 2550
BstX2I RGATCY 1 cut(s) 2394
BstYI RGATCY 1 cut(s) 2394
BstZ17I GTATAC 1 cut(s) 747
BstZI CGGCCG 1 cut(s) 512
BsuRI GGCC 7 cut(s) 135, 193, 425, 514, 1072, 1409, 1741
BtsCI GGATG 9 cut(s) 13, 95, 1209, 1229, 1756, 1855, 1882, 2079, 2137
BtsI GCAGTG 1 cut(s) 1169
BtsIMutI CAGTG 6 cut(s) 194, 348, 704, 1169, 1664, 2494
CfoI GCGC 4 cut(s) 244, 367, 391, 2527
Cfr13I GGNCC 6 cut(s) 133, 134, 430, 1070, 1204, 2766
Cfr9I CCCGGG 1 cut(s) 130
CseI GACGC 8 cut(s) 113, 148, 165, 395, 533, 1997, 2318, 2551
CsiI ACCWGGT 1 cut(s) 1672
Csp6I GTAC 4 cut(s) 590, 784, 1301, 1978
CviAII CATG 8 cut(s) 368, 1020, 1049, 1108, 1363, 1547, 1631, 2771
CviQI GTAC 4 cut(s) 590, 784, 1301, 1978
DpnI GATC 8 cut(s) 204, 401, 833, 1479, 2186, 2287, 2396, 2429
DpnII GATC 8 cut(s) 202, 399, 831, 1477, 2184, 2285, 2394, 2427
DrdI GACNNNNNNGTC 1 cut(s) 535
DriI GACNNNNNGTC 1 cut(s) 25
DseDI GACNNNNNNGTC 1 cut(s) 535
EaeI YGGCCR 3 cut(s) 191, 512, 1407
EagI CGGCCG 1 cut(s) 512
Eam1104I CTCTTC 7 cut(s) 146, 496, 860, 933, 1178, 2304, 2580
Eam1105I GACNNNNNGTC 1 cut(s) 25
EarI CTCTTC 7 cut(s) 146, 496, 860, 933, 1178, 2304, 2580
EciI GGCGGA 1 cut(s) 541
EclXI CGGCCG 1 cut(s) 512
Eco130I CCWWGG 1 cut(s) 1073
Eco24I GRGCYC 3 cut(s) 53, 114, 137
Eco31I GGTCTC 2 cut(s) 1429, 2476
Eco32I GATATC 1 cut(s) 2702
Eco47I GGWCC 3 cut(s) 430, 1204, 2766
Eco52I CGGCCG 1 cut(s) 512
Eco57I CTGAAG 5 cut(s) 1023, 1436, 2400, 2562, 2604
Eco72I CACGTG 2 cut(s) 1442, 1493
Eco88I CYCGRG 2 cut(s) 130, 1982
EcoNI CCTNNNNNAGG 1 cut(s) 1418
EcoRII CCWGG 2 cut(s) 1672, 2634
EcoRV GATATC 1 cut(s) 2702
EcoT14I CCWWGG 1 cut(s) 1073
EcoT38I GRGCYC 3 cut(s) 53, 114, 137
ErhI CCWWGG 1 cut(s) 1073
Esp3I CGTCTC 2 cut(s) 166, 2336
FaeI CATG 8 cut(s) 371, 1023, 1052, 1111, 1366, 1550, 1634, 2774
FalI AAGNNNNNCTT 2 cut(s) 1823, 1855
FaqI GGGAC 6 cut(s) 284, 416, 1217, 1381, 1908, 2752
FatI CATG 8 cut(s) 367, 1019, 1048, 1107, 1362, 1546, 1630, 2770
FauI CCCGC 4 cut(s) 154, 191, 217, 253
FauNDI CATATG 1 cut(s) 910
FbaI TGATCA 1 cut(s) 2184
FblI GTMKAC 2 cut(s) 506, 746
FokI GGATG 9 cut(s) 20, 82, 1196, 1236, 1763, 1862, 1889, 2086, 2144
FriOI GRGCYC 3 cut(s) 53, 114, 137
FspBI CTAG 9 cut(s) 498, 557, 1259, 2039, 2139, 2255, 2454, 2460, 2481
GlaI GCGC 4 cut(s) 243, 366, 390, 2526
HaeII RGCGCY 1 cut(s) 2528
HaeIII GGCC 7 cut(s) 135, 193, 425, 514, 1072, 1409, 1741
HapII CCGG 5 cut(s) 131, 440, 610, 1847, 2057
HgaI GACGC 8 cut(s) 113, 148, 165, 395, 533, 1997, 2318, 2551
HhaI GCGC 4 cut(s) 244, 367, 391, 2527
Hin1I GRCGYC 1 cut(s) 2008
Hin1II CATG 8 cut(s) 371, 1023, 1052, 1111, 1366, 1550, 1634, 2774
Hin6I GCGC 4 cut(s) 242, 365, 389, 2525
HinP1I GCGC 4 cut(s) 242, 365, 389, 2525
HincII GTYRAC 1 cut(s) 507
HindII GTYRAC 1 cut(s) 507
HindIII AAGCTT 4 cut(s) 1346, 1592, 2156, 2412
HpaII CCGG 5 cut(s) 131, 440, 610, 1847, 2057
HphI GGTGA 3 cut(s) 76, 1537, 1600
Hpy166II GTNNAC 7 cut(s) 385, 507, 747, 1244, 2126, 2164, 2713
Hpy8I GTNNAC 7 cut(s) 385, 507, 747, 1244, 2126, 2164, 2713
Hpy99I CGWCG 6 cut(s) 28, 31, 104, 107, 159, 2013
HpyCH4IV ACGT 2 cut(s) 1441, 1492
HpyF10VI GCNNNNNNNGC 5 cut(s) 368, 494, 977, 1389, 2537
HpySE526I ACGT 2 cut(s) 1441, 1492
Hsp92I GRCGYC 1 cut(s) 2008
Hsp92II CATG 8 cut(s) 371, 1023, 1052, 1111, 1366, 1550, 1634, 2774
HspAI GCGC 4 cut(s) 242, 365, 389, 2525
Kpn2I TCCGGA 1 cut(s) 1846
Ksp22I TGATCA 1 cut(s) 2184
Kzo9I GATC 8 cut(s) 202, 399, 831, 1477, 2184, 2285, 2394, 2427
LguI GCTCTTC 1 cut(s) 933
LmnI GCTCC 5 cut(s) 109, 336, 1330, 1921, 2572
Lsp1109I GCAGC 6 cut(s) 70, 681, 1267, 1404, 1804, 2419
LweI GCATC 7 cut(s) 104, 1074, 1116, 1135, 1469, 1477, 1780
MabI ACCWGGT 1 cut(s) 1672
MaeI CTAG 9 cut(s) 498, 557, 1259, 2039, 2139, 2255, 2454, 2460, 2481
MaeII ACGT 2 cut(s) 1441, 1492
MaeIII GTNAC 4 cut(s) 552, 641, 1229, 1565
MalI GATC 8 cut(s) 204, 401, 833, 1479, 2186, 2287, 2396, 2429
MboI GATC 8 cut(s) 202, 399, 831, 1477, 2184, 2285, 2394, 2427
MflI RGATCY 1 cut(s) 2394
MhlI GDGCHC 6 cut(s) 53, 114, 137, 567, 1512, 1666
MluI ACGCGT 1 cut(s) 157
MlyI GAGTC 6 cut(s) 502, 512, 545, 826, 2255, 2273
MmeI TCCRAC 5 cut(s) 24, 123, 178, 291, 1579
MroI TCCGGA 1 cut(s) 1846
MseI TTAA 8 cut(s) 675, 1809, 1841, 2154, 2234, 2556, 2607, 2733
MslI CAYNNNNRTG 3 cut(s) 717, 1687, 2775
MspI CCGG 5 cut(s) 131, 440, 610, 1847, 2057
MspR9I CCNGG 5 cut(s) 131, 132, 1674, 2058, 2636
Mva1269I GAATGC 2 cut(s) 1720, 2045
MvaI CCWGG 2 cut(s) 1674, 2636
MvnI CGCG 7 cut(s) 107, 159, 273, 321, 365, 389, 2562
MwoI GCNNNNNNNGC 5 cut(s) 368, 494, 977, 1389, 2537
NciI CCSGG 3 cut(s) 131, 132, 2058
NdeI CATATG 1 cut(s) 910
NdeII GATC 8 cut(s) 202, 399, 831, 1477, 2184, 2285, 2394, 2427
NheI GCTAGC 1 cut(s) 1258
NlaIII CATG 8 cut(s) 371, 1023, 1052, 1111, 1366, 1550, 1634, 2774
NlaIV GGNNCC 8 cut(s) 111, 135, 432, 471, 855, 1205, 2396, 2768
NmeAIII GCCGAG 2 cut(s) 490, 540
NmuCI GTSAC 2 cut(s) 1229, 1565
NspI RCATGY 2 cut(s) 371, 1111
PaeI GCATGC 2 cut(s) 371, 1111
PciSI GCTCTTC 1 cut(s) 933
PcsI WCGNNNNNNNCGW 2 cut(s) 32, 512
PctI GAATGC 2 cut(s) 1720, 2045
Ple19I CGATCG 1 cut(s) 205
PleI GAGTC 6 cut(s) 502, 511, 544, 825, 2255, 2273
PmaCI CACGTG 2 cut(s) 1442, 1493
PmlI CACGTG 2 cut(s) 1442, 1493
PpsI GAGTC 6 cut(s) 502, 511, 544, 825, 2255, 2273
Ppu21I YACGTR 2 cut(s) 1442, 1493
PshBI ATTAAT 3 cut(s) 2234, 2556, 2733
Psp6I CCWGG 2 cut(s) 1672, 2634
PspCI CACGTG 2 cut(s) 1442, 1493
PspGI CCWGG 2 cut(s) 1672, 2634
PspN4I GGNNCC 8 cut(s) 111, 135, 432, 471, 855, 1205, 2396, 2768
PspOMI GGGCCC 1 cut(s) 133
PspPI GGNCC 6 cut(s) 133, 134, 430, 1070, 1204, 2766
PstI CTGCAG 1 cut(s) 1822
PsuI RGATCY 1 cut(s) 2394
PvuI CGATCG 1 cut(s) 205
RsaI GTAC 4 cut(s) 591, 785, 1302, 1979
RsaNI GTAC 4 cut(s) 590, 784, 1301, 1978
RseI CAYNNNNRTG 3 cut(s) 717, 1687, 2775
SalI GTCGAC 1 cut(s) 505
SapI GCTCTTC 1 cut(s) 933
SaqAI TTAA 8 cut(s) 675, 1809, 1841, 2154, 2234, 2556, 2607, 2733
Sau3AI GATC 8 cut(s) 202, 399, 831, 1477, 2184, 2285, 2394, 2427
Sau96I GGNCC 6 cut(s) 133, 134, 430, 1070, 1204, 2766
SchI GAGTC 6 cut(s) 502, 512, 545, 826, 2255, 2273
ScrFI CCNGG 5 cut(s) 131, 132, 1674, 2058, 2636
SduI GDGCHC 6 cut(s) 53, 114, 137, 567, 1512, 1666
SexAI ACCWGGT 1 cut(s) 1672
SfaNI GCATC 7 cut(s) 104, 1074, 1116, 1135, 1469, 1477, 1780
SfcI CTRYAG 1 cut(s) 1818
SinI GGWCC 3 cut(s) 430, 1204, 2766
SmaI CCCGGG 1 cut(s) 132
SmiMI CAYNNNNRTG 3 cut(s) 717, 1687, 2775
SmlI CTYRAG 5 cut(s) 62, 629, 1537, 2281, 2567
SmoI CTYRAG 5 cut(s) 62, 629, 1537, 2281, 2567
SpeI ACTAGT 1 cut(s) 556
SphI GCATGC 2 cut(s) 371, 1111
SspI AATATT 1 cut(s) 1116
SspMI CTAG 9 cut(s) 498, 557, 1259, 2039, 2139, 2255, 2454, 2460, 2481
StyD4I CCNGG 5 cut(s) 129, 130, 1672, 2056, 2634
StyI CCWWGG 1 cut(s) 1073
TaiI ACGT 2 cut(s) 1444, 1495
TaqI TCGA 5 cut(s) 29, 506, 521, 761, 2426
TaqII GACCGA 1 cut(s) 418
TatI WGTACW 2 cut(s) 783, 1300
TauI GCSGC 7 cut(s) 196, 199, 324, 365, 394, 425, 2004
Tru1I TTAA 8 cut(s) 675, 1809, 1841, 2154, 2234, 2556, 2607, 2733
Tru9I TTAA 8 cut(s) 675, 1809, 1841, 2154, 2234, 2556, 2607, 2733
TscAI CASTG 6 cut(s) 194, 355, 711, 1176, 1664, 2494
TseFI GTSAC 2 cut(s) 1229, 1565
TseI GCWGC 6 cut(s) 58, 669, 1255, 1392, 1817, 2407
Tsp45I GTSAC 2 cut(s) 1229, 1565
TspGWI ACGGA 4 cut(s) 412, 844, 889, 2387
TspMI CCCGGG 1 cut(s) 130
TspRI CASTG 6 cut(s) 194, 355, 711, 1176, 1664, 2494
VpaK11BI GGWCC 3 cut(s) 430, 1204, 2766
VspI ATTAAT 3 cut(s) 2234, 2556, 2733
XagI CCTNNNNNAGG 1 cut(s) 1418
XapI RAATTY 2 cut(s) 1352, 2442
XbaI TCTAGA 3 cut(s) 2038, 2138, 2480
XceI RCATGY 2 cut(s) 371, 1111
XmaI CCCGGG 1 cut(s) 130
XmiI GTMKAC 2 cut(s) 506, 746
XspI CTAG 9 cut(s) 498, 557, 1259, 2039, 2139, 2255, 2454, 2460, 2481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.