Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0002058

Physical Location & Seq

Genomic Coordinates Forward (+)

24718009 .. 24722985

Gene Structure 4977 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g21720.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1521 bp

ATGATCATGGCGTTTAAGTGGGTTCTAGTTGTAATTTGGATATGGGTTTGGGCAGCTTACTTGGCTGCTACGCAATCCGTTGATGGGTTAGTGAGGATTAGTCTAAAGAAGCATCCTTTAGACTTAAGTAGGATAAATGCTGCAAGGATCACCAATAGAGTAGCTTATCAACAGAGTGATAATGGGGATAATCTCAAAGCTAACATTGTGTATTTGAAGAATTATCTTGATATCCAATTTTATGGAGAAATTGGTATTGGGTCACCCACACAAACCTTTGCTGTTGTCTTTGATACTGGAAGCTCCAATCTTTGGGTCCCCTCGTCGAGATGCGTCCTTTCTGTTGGTTGCTATTTTCATTCTAAGTACAGGGCAACAATGTCAAGTACCTATACCAAAATTGGAATACATTGCAAAATTCCTTATGGCTCTGGTTCTGTTTCCGGCTACTTCAGTGGGGATAATGTTAAAGTTGGGGATATCATCGTTAAAGATCAAGAATTTGTTGAGGTTACAAGGGAAGGGTTTGTAGCATTCTTAGCTGCACGGTTTGATGGGGTTTTTGGACTTGGATTTCAAGACATTTCTGTTGGTGAAGCCACACCAGTGTGGTATAACATGGTGCAACAAGGTCGTATGAGCCAGAAGATTTTCTCTATTTGGCTCAATCATGATCCAAAATCACCAGTGGGAGGTGAAATCGTCTTTGGAGGATATGATTGGAAGCACTTTACGGGTGATCACACTTATGTCCCGATTTCACATAAGGGATACTGGCAGATTGAGGTGGGAGATATCATTGTTGCCGACTTCTCAACAGGATTCTGCAAGGGTGGCTGTGCTGCCATTGTGGACTCAGGGACATCCTTACTTGCTGGTCCAACTACTGTTGTGGCTCAGATCAACCATGCCATCGGAGCAGAAGGTTTGGCCAGCTTGGAATGTAAAAATGTAGTTTACACTTATGGAAACCTGATGTGGGATTATTTAGTATCTGGGGTACGGCCTGATGTACTGTGTGTGGATATCAGCCTTTGTTCATATGATGGACCTCATCATGTAGACAATTATATTGAGACAGTAGTTCGAAATAGAACTCAGAAGGAGGCATCTGTTAACGAAACTCCTTTATGTACTTTGTGTGAGATGATTGCCTACTGGATTCAACTTCAACTTAGAAAACAAGTATCGAGGGAACAAGTATTTAAGCATGTAAATGAGCTGTGTGAGAAGCTTCCAAATCCTGTGCGAAGATCGTTTGTCAACTGCGATAACCTTGCAGCCATGCCAGATGTTTCCTTCGTGATTGGAAACAAAACCTTCACCCTCACTCCAGAACAGTATATACTTAAAGTTGAAGAAAGGTGTTCTACTGTCTGCCTTAGCGGATTTGTTGCCTTGGATGTGCCTCCTCCACAAGGTCCACTATGGGTTCTTGGAGCTCTGTTCCTGGGTGCATACCACACTGTGTTTGACTTTGGTAATCTCAGGGTAGGATTTGCCAAAGCTGCAATCCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0044238 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asp SapB_1 SapB_2

Protein Analysis

507

Amino Acids

56.07

Weight (kDa)

6.31

Isoelectric Point (pI)

28.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	79 - 469	1.4e-90	Eukaryotic aspartyl protease
TAXi_N	PF14543	81 - 238	1.3e-06	Xylanase inhibitor N-terminal
SapB_2	PF03489	314 - 346	9.2e-07	Saposin-like type B, region 2
SapB_1	PF05184	376 - 413	2.5e-07	Saposin-like type B, region 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002058)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G22050
fragaria_vesca	FvH4_4g21720 FvH4_4g21720 FvH4_4g21720 FvH4_6g08500 FvH4_6g08500 FvH4_6g08500 FvH4_6g08500
malus_domestica	MD04G1186500.v1.1 MD04G1186600.v1.1 MD13G1138100.v1.1 MD16G1133900.v1.1
prunus_persica	Prupe.1G103000_v2.0.a1 Prupe.1G103000_v2.0.a1 Prupe.1G103000_v2.0.a1 Prupe.1G103000_v2.0.a1 Prupe.6G304600_v2.0.a1 Prupe.6G304600_v2.0.a1 Prupe.6G304600_v2.0.a1
pyrus_communis	pycom04g16480 pycom13g12020 pycom16g11820
rosa_chinensis	RchiOBHm_Chr3g0458541 RchiOBHm_Chr4g0427921
rosa_laevigata	RLG00000007200 RLG00000025149
rosa_roxburghii	Rroxscaffold_2G00125080 Rroxscaffold_5G00369590 Rroxscaffold_6G00420990
rosa_rugosa	Rorug03G0029500 Rorug03G0029500 Rorug03G0029500 Rorug03G0029600 Rorug04G0220600
rosa_samantha	Rh3AG087900 Rh3BG091000 Rh3CG091100 Rh3DG091900 Rh4AG277600 Rh4BG283400 Rh4CG298900 Rh4DG280600
rosa_wichuraiana	Rw3G007510 Rw4G024120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	312
AccI	GTMKAC	1 cut(s)	1062
AciI	CCGC	1 cut(s)	1386
AclWI	GGATC	2 cut(s)	155, 668
AcoI	YGGCCR	1 cut(s)	930
AcsI	RAATTY	2 cut(s)	417, 500
AcuI	CTGAAG	1 cut(s)	436
AdeI	CACNNNGTG	1 cut(s)	1468
AfaI	GTAC	5 cut(s)	368, 388, 1002, 1014, 1135
AfiI	CCNNNNNNNGG	5 cut(s)	84, 312, 692, 979, 1418
AflII	CTTAAG	1 cut(s)	124
AgsI	TTSAA	5 cut(s)	217, 578, 1166, 1172, 1358
AjnI	CCWGG	1 cut(s)	1449
AjuI	GAANNNNNNNTTGG	4 cut(s)	240, 272, 690, 722
AleI	CACNNNNGTG	2 cut(s)	605, 607
Alw21I	GWGCWC	1 cut(s)	1444
Alw26I	GTCTC	1 cut(s)	1070
AlwI	GGATC	2 cut(s)	155, 668
AoxI	GGCC	2 cut(s)	930, 1004
ApeKI	GCWGC	7 cut(s)	53, 65, 140, 542, 842, 1280, 1508
ApoI	RAATTY	2 cut(s)	417, 500
Asp700I	GAANNNNTTC	1 cut(s)	650
AspS9I	GGNCC	4 cut(s)	316, 878, 1049, 1421
AsuHPI	GGTGA	7 cut(s)	142, 255, 605, 675, 707, 749, 1315
AsuII	TTCGAA	1 cut(s)	1087
AvaII	GGWCC	4 cut(s)	316, 878, 1049, 1421
BalI	TGGCCA	1 cut(s)	932
BanII	GRGCYC	1 cut(s)	1444
Bbv12I	GWGCWC	1 cut(s)	1444
BbvI	GCAGC	7 cut(s)	52, 65, 127, 529, 829, 1292, 1495
BccI	CCATC	4 cut(s)	77, 548, 920, 1040
BceAI	ACGGC	1 cut(s)	1019
BcgI	CGANNNNNNTGC	4 cut(s)	614, 648, 1259, 1293
BciT130I	CCWGG	1 cut(s)	1451
BciVI	GTATCC	1 cut(s)	764
BclI	TGATCA	2 cut(s)	3, 739
BcoDI	GTCTC	1 cut(s)	1070
BfaI	CTAG	1 cut(s)	26
BfrI	CTTAAG	1 cut(s)	124
BfuI	GTATCC	1 cut(s)	764
BisI	GCNGC	7 cut(s)	54, 66, 141, 543, 843, 1281, 1509
BlsI	GCNGC	7 cut(s)	55, 67, 142, 544, 844, 1282, 1510
Bme1390I	CCNGG	1 cut(s)	1451
Bme18I	GGWCC	4 cut(s)	316, 878, 1049, 1421
BmgT120I	GGNCC	4 cut(s)	316, 878, 1049, 1421
BmiI	GGNNCC	2 cut(s)	317, 318
BmrFI	CCNGG	1 cut(s)	1451
BmsI	GCATC	3 cut(s)	121, 320, 1118
BpmI	CTGGAG	1 cut(s)	1317
Bpu10I	CCTNAGC	1 cut(s)	1382
Bpu14I	TTCGAA	1 cut(s)	1087
BsaJI	CCNNGG	2 cut(s)	1398, 1450
BsaXI	ACNNNNNCTCC	2 cut(s)	1315, 1345
Bsc4I	CCNNNNNNNGG	5 cut(s)	84, 312, 692, 979, 1418
Bse1I	ACTGG	5 cut(s)	301, 605, 686, 779, 1163
Bse3DI	GCAATG	1 cut(s)	409
BseBI	CCWGG	1 cut(s)	1451
BseDI	CCNNGG	2 cut(s)	1398, 1450
BseGI	GGATG	3 cut(s)	112, 863, 1408
BseLI	CCNNNNNNNGG	5 cut(s)	84, 312, 692, 979, 1418
BseMI	GCAATG	1 cut(s)	409
BseMII	CTCAG	4 cut(s)	870, 911, 1112, 1501
BseNI	ACTGG	5 cut(s)	301, 605, 686, 779, 1163
BseRI	GAGGAG	1 cut(s)	1401
BseXI	GCAGC	7 cut(s)	52, 65, 127, 529, 829, 1292, 1495
BsgI	GTGCAG	1 cut(s)	528
BshFI	GGCC	2 cut(s)	932, 1006
BsiHKAI	GWGCWC	1 cut(s)	1444
BsiSI	CCGG	1 cut(s)	444
BslFI	GGGAC	3 cut(s)	302, 737, 874
BslI	CCNNNNNNNGG	5 cut(s)	84, 312, 692, 979, 1418
BsmAI	GTCTC	1 cut(s)	1070
BsmFI	GGGAC	3 cut(s)	302, 737, 874
BsmI	GAATGC	1 cut(s)	533
BsnI	GGCC	2 cut(s)	932, 1006
Bsp119I	TTCGAA	1 cut(s)	1087
Bsp1286I	GDGCHC	1 cut(s)	1444
Bsp143I	GATC	7 cut(s)	3, 147, 493, 673, 739, 900, 1253
BspACI	CCGC	1 cut(s)	1386
BspANI	GGCC	2 cut(s)	932, 1006
BspCNI	CTCAG	4 cut(s)	869, 910, 1111, 1500
BspHI	TCATGA	1 cut(s)	670
BspLI	GGNNCC	2 cut(s)	317, 318
BspPI	GGATC	2 cut(s)	155, 668
BspT104I	TTCGAA	1 cut(s)	1087
BspTI	CTTAAG	1 cut(s)	124
BsrDI	GCAATG	1 cut(s)	409
BsrI	ACTGG	5 cut(s)	301, 605, 686, 779, 1163
BssECI	CCNNGG	2 cut(s)	1398, 1450
BssMI	GATC	7 cut(s)	3, 147, 493, 673, 739, 900, 1253
BssT1I	CCWWGG	1 cut(s)	1398
Bst2UI	CCWGG	1 cut(s)	1451
Bst4CI	ACNGT	7 cut(s)	549, 889, 1017, 1081, 1341, 1375, 1468
BstAFI	CTTAAG	1 cut(s)	124
BstBI	TTCGAA	1 cut(s)	1087
BstC8I	GCNNGC	1 cut(s)	934
BstDEI	CTNAG	8 cut(s)	363, 538, 856, 897, 1098, 1175, 1382, 1487
BstEII	GGTNACC	1 cut(s)	261
BstENI	CCTNNNNNAGG	1 cut(s)	1416
BstF5I	GGATG	3 cut(s)	112, 863, 1408
BstKTI	GATC	7 cut(s)	6, 150, 496, 676, 742, 903, 1256
BstMAI	GTCTC	1 cut(s)	1070
BstMBI	GATC	7 cut(s)	3, 147, 493, 673, 739, 900, 1253
BstMWI	GCNNNNNNNGC	5 cut(s)	62, 539, 834, 917, 1508
BstNI	CCWGG	1 cut(s)	1451
BstNSI	RCATGY	1 cut(s)	1214
BstPI	GGTNACC	1 cut(s)	261
BstSCI	CCNGG	1 cut(s)	1449
BstV1I	GCAGC	7 cut(s)	52, 65, 127, 529, 829, 1292, 1495
BstXI	CCANNNNNNTGG	1 cut(s)	242
BsuI	GTATCC	1 cut(s)	764
BsuRI	GGCC	2 cut(s)	932, 1006
BtsCI	GGATG	3 cut(s)	112, 863, 1408
BtsIMutI	CAGTG	4 cut(s)	460, 612, 693, 1464
Cac8I	GCNNGC	1 cut(s)	934
CciI	TCATGA	1 cut(s)	670
Cfr13I	GGNCC	4 cut(s)	316, 878, 1049, 1421
CseI	GACGC	1 cut(s)	322
Csp6I	GTAC	5 cut(s)	367, 387, 1001, 1013, 1134
CviAII	CATG	7 cut(s)	7, 619, 671, 908, 1058, 1211, 1285
CviQI	GTAC	5 cut(s)	367, 387, 1001, 1013, 1134
DdeI	CTNAG	8 cut(s)	363, 538, 856, 897, 1098, 1175, 1382, 1487
DpnI	GATC	7 cut(s)	5, 149, 495, 675, 741, 902, 1255
DpnII	GATC	7 cut(s)	3, 147, 493, 673, 739, 900, 1253
DraIII	CACNNNGTG	1 cut(s)	1468
EaeI	YGGCCR	1 cut(s)	930
Ecl136II	GAGCTC	1 cut(s)	1442
Eco130I	CCWWGG	1 cut(s)	1398
Eco24I	GRGCYC	1 cut(s)	1444
Eco32I	GATATC	4 cut(s)	232, 481, 796, 1027
Eco47I	GGWCC	4 cut(s)	316, 878, 1049, 1421
Eco53kI	GAGCTC	1 cut(s)	1442
Eco57I	CTGAAG	1 cut(s)	436
Eco91I	GGTNACC	1 cut(s)	261
EcoICRI	GAGCTC	1 cut(s)	1442
EcoNI	CCTNNNNNAGG	1 cut(s)	1416
EcoO109I	RGGNCCY	1 cut(s)	316
EcoO65I	GGTNACC	1 cut(s)	261
EcoRII	CCWGG	1 cut(s)	1449
EcoRV	GATATC	4 cut(s)	232, 481, 796, 1027
EcoT14I	CCWWGG	1 cut(s)	1398
EcoT38I	GRGCYC	1 cut(s)	1444
ErhI	CCWWGG	1 cut(s)	1398
FaeI	CATG	7 cut(s)	10, 622, 674, 911, 1061, 1214, 1288
FaqI	GGGAC	3 cut(s)	302, 737, 874
FatI	CATG	7 cut(s)	6, 618, 670, 907, 1057, 1210, 1284
FauNDI	CATATG	1 cut(s)	1042
FbaI	TGATCA	2 cut(s)	3, 739
FblI	GTMKAC	1 cut(s)	1062
Fnu4HI	GCNGC	7 cut(s)	54, 66, 141, 543, 843, 1281, 1509
FokI	GGATG	3 cut(s)	99, 850, 1415
FriOI	GRGCYC	1 cut(s)	1444
Fsp4HI	GCNGC	7 cut(s)	54, 66, 141, 543, 843, 1281, 1509
FspBI	CTAG	1 cut(s)	26
GluI	GCNGC	7 cut(s)	54, 66, 141, 543, 843, 1281, 1509
GsuI	CTGGAG	1 cut(s)	1317
HaeIII	GGCC	2 cut(s)	932, 1006
HapII	CCGG	1 cut(s)	444
HgaI	GACGC	1 cut(s)	322
Hin1II	CATG	7 cut(s)	10, 622, 674, 911, 1061, 1214, 1288
HincII	GTYRAC	2 cut(s)	1117, 1264
HindII	GTYRAC	2 cut(s)	1117, 1264
HindIII	AAGCTT	1 cut(s)	1232
HinfI	GANTC	3 cut(s)	822, 854, 1162
HpaI	GTTAAC	1 cut(s)	1117
HpaII	CCGG	1 cut(s)	444
HphI	GGTGA	7 cut(s)	142, 255, 605, 675, 707, 749, 1315
Hpy166II	GTNNAC	6 cut(s)	853, 958, 1063, 1117, 1264, 1424
Hpy188I	TCNGA	3 cut(s)	900, 917, 1101
Hpy188III	TCNNGA	8 cut(s)	227, 327, 497, 578, 671, 754, 1303, 1334
Hpy8I	GTNNAC	6 cut(s)	853, 958, 1063, 1117, 1264, 1424
Hpy99I	CGWCG	1 cut(s)	328
HpyAV	CCTTC	5 cut(s)	515, 917, 1096, 1309, 1330
HpyCH4III	ACNGT	7 cut(s)	549, 889, 1017, 1081, 1341, 1375, 1468
HpyCH4V	TGCA	8 cut(s)	143, 414, 545, 625, 828, 1280, 1457, 1511
HpyF10VI	GCNNNNNNNGC	5 cut(s)	62, 539, 834, 917, 1508
HpyF3I	CTNAG	8 cut(s)	363, 538, 856, 897, 1098, 1175, 1382, 1487
Hsp92II	CATG	7 cut(s)	10, 622, 674, 911, 1061, 1214, 1288
KflI	GGGWCCC	1 cut(s)	316
Ksp22I	TGATCA	2 cut(s)	3, 739
KspAI	GTTAAC	1 cut(s)	1117
Kzo9I	GATC	7 cut(s)	3, 147, 493, 673, 739, 900, 1253
LmnI	GCTCC	3 cut(s)	308, 917, 1439
Lsp1109I	GCAGC	7 cut(s)	52, 65, 127, 529, 829, 1292, 1495
LweI	GCATC	3 cut(s)	121, 320, 1118
MaeI	CTAG	1 cut(s)	26
MaeIII	GTNAC	2 cut(s)	261, 511
MalI	GATC	7 cut(s)	5, 149, 495, 675, 741, 902, 1255
MboI	GATC	7 cut(s)	3, 147, 493, 673, 739, 900, 1253
MboII	GAAGA	4 cut(s)	229, 658, 1263, 1370
MhlI	GDGCHC	1 cut(s)	1444
MlsI	TGGCCA	1 cut(s)	932
MluCI	AATT	8 cut(s)	33, 220, 236, 249, 399, 417, 500, 1066
MluNI	TGGCCA	1 cut(s)	932
MlyI	GAGTC	1 cut(s)	848
MmeI	TCCRAC	1 cut(s)	905
Mox20I	TGGCCA	1 cut(s)	932
MroXI	GAANNNNTTC	1 cut(s)	650
MscI	TGGCCA	1 cut(s)	932
MseI	TTAA	7 cut(s)	15, 125, 468, 489, 1116, 1206, 1350
MslI	CAYNNNNRTG	4 cut(s)	605, 607, 747, 1215
Msp20I	TGGCCA	1 cut(s)	932
MspCI	CTTAAG	1 cut(s)	124
MspI	CCGG	1 cut(s)	444
MspR9I	CCNGG	1 cut(s)	1451
Mva1269I	GAATGC	1 cut(s)	533
MvaI	CCWGG	1 cut(s)	1451
MwoI	GCNNNNNNNGC	5 cut(s)	62, 539, 834, 917, 1508
NdeI	CATATG	1 cut(s)	1042
NdeII	GATC	7 cut(s)	3, 147, 493, 673, 739, 900, 1253
NlaIII	CATG	7 cut(s)	10, 622, 674, 911, 1061, 1214, 1288
NlaIV	GGNNCC	2 cut(s)	317, 318
NmuCI	GTSAC	1 cut(s)	261
NspI	RCATGY	1 cut(s)	1214
NspV	TTCGAA	1 cut(s)	1087
OliI	CACNNNNGTG	2 cut(s)	605, 607
PagI	TCATGA	1 cut(s)	670
PctI	GAATGC	1 cut(s)	533
PdmI	GAANNNNTTC	1 cut(s)	650
PfeI	GAWTC	2 cut(s)	822, 1162
PflMI	CCANNNNNTGG	1 cut(s)	312
PkrI	GCNGC	7 cut(s)	55, 67, 142, 544, 844, 1282, 1510
PleI	GAGTC	1 cut(s)	848
PpsI	GAGTC	1 cut(s)	848
PpuMI	RGGWCCY	1 cut(s)	316
Psp124BI	GAGCTC	1 cut(s)	1444
Psp5II	RGGWCCY	1 cut(s)	316
Psp6I	CCWGG	1 cut(s)	1449
PspEI	GGTNACC	1 cut(s)	261
PspGI	CCWGG	1 cut(s)	1449
PspN4I	GGNNCC	2 cut(s)	317, 318
PspPI	GGNCC	4 cut(s)	316, 878, 1049, 1421
PspPPI	RGGWCCY	1 cut(s)	316
PsrI	GAACNNNNNNTAC	2 cut(s)	1329, 1361
RsaI	GTAC	5 cut(s)	368, 388, 1002, 1014, 1135
RsaNI	GTAC	5 cut(s)	367, 387, 1001, 1013, 1134
RseI	CAYNNNNRTG	4 cut(s)	605, 607, 747, 1215
SacI	GAGCTC	1 cut(s)	1444
SaqAI	TTAA	7 cut(s)	15, 125, 468, 489, 1116, 1206, 1350
SatI	GCNGC	7 cut(s)	54, 66, 141, 543, 843, 1281, 1509
Sau3AI	GATC	7 cut(s)	3, 147, 493, 673, 739, 900, 1253
Sau96I	GGNCC	4 cut(s)	316, 878, 1049, 1421
SchI	GAGTC	1 cut(s)	848
ScrFI	CCNGG	1 cut(s)	1451
SduI	GDGCHC	1 cut(s)	1444
SfaNI	GCATC	3 cut(s)	121, 320, 1118
SfuI	TTCGAA	1 cut(s)	1087
SinI	GGWCC	4 cut(s)	316, 878, 1049, 1421
SmiMI	CAYNNNNRTG	4 cut(s)	605, 607, 747, 1215
SmlI	CTYRAG	1 cut(s)	124
SmoI	CTYRAG	1 cut(s)	124
Sse9I	AATT	8 cut(s)	33, 220, 236, 249, 399, 417, 500, 1066
SsiI	CCGC	1 cut(s)	1386
SspMI	CTAG	1 cut(s)	26
SstI	GAGCTC	1 cut(s)	1444
StyD4I	CCNGG	1 cut(s)	1449
StyI	CCWWGG	1 cut(s)	1398
TaaI	ACNGT	7 cut(s)	549, 889, 1017, 1081, 1341, 1375, 1468
TaqI	TCGA	3 cut(s)	326, 1087, 1190
TasI	AATT	8 cut(s)	33, 220, 236, 249, 399, 417, 500, 1066
TatI	WGTACW	3 cut(s)	366, 1012, 1133
TfiI	GAWTC	2 cut(s)	822, 1162
Tru1I	TTAA	7 cut(s)	15, 125, 468, 489, 1116, 1206, 1350
Tru9I	TTAA	7 cut(s)	15, 125, 468, 489, 1116, 1206, 1350
TscAI	CASTG	4 cut(s)	460, 612, 693, 1471
TseFI	GTSAC	1 cut(s)	261
TseI	GCWGC	7 cut(s)	53, 65, 140, 542, 842, 1280, 1508
Tsp45I	GTSAC	1 cut(s)	261
TspDTI	ATGAA	2 cut(s)	347, 1029
TspGWI	ACGGA	1 cut(s)	67
TspRI	CASTG	4 cut(s)	460, 612, 693, 1471
Van91I	CCANNNNNTGG	1 cut(s)	312
Vha464I	CTTAAG	1 cut(s)	124
VpaK11BI	GGWCC	4 cut(s)	316, 878, 1049, 1421
XagI	CCTNNNNNAGG	1 cut(s)	1416
XapI	RAATTY	2 cut(s)	417, 500
XceI	RCATGY	1 cut(s)	1214
XmiI	GTMKAC	1 cut(s)	1062
XmnI	GAANNNNTTC	1 cut(s)	650
XspI	CTAG	1 cut(s)	26

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.