Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0002058

Physical Location & Seq

Genomic Coordinates Reverse (-)

10281355 .. 10284631

Gene Structure 3277 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1133900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1593 bp

ATGAGGTTGGGGTCCAACTGCACAGCTGTGGTGAAATTCAGTCTACTAAAATTGTATTTTATTATTTTCCATGGCAGCCACAGAAGTCTGAAAATGAGGTTTAAGGGACTTGTGGTTGCAATTTGTATGTGGGTTTGGTCAGGCTCCTTGGCTGCTGATACAAAATCCAGTGATGGGTTACTGAGAATTGGTCTAAAGAAGCAGCCTTTGGACCTCACCAGATTGAATGCTGCAAGAATCACCAGGTCACAAGTTTCCCAACCCAGTGATCTCAAATCCAACATTGTGGATCTGAAAAATTATCGGGACACCCAGTTTTATGGGGACATTGCTATTGGCACCCCACCACAGCACTTTGCTGTTGTCTTTGACACCGGCAGCTCCAATCTGTGGGTCCCATCTTCAAGATGCATCCTTTCTATGTCTTGCTATTTTCATTCTAAATACAGGGCAAGCCTTTCGAGCACTTATACCAAAATTGGAATACCCTCCAAAATTCCTTATGGGTCTGGTTCGATTTCCGGCTACTTTAGTGAAGATAATTTGAAAATTGGGGATGTTATCGTCAGAGATCAAGAATTTGTTGAGATTACAAGGGAAGGATTCCTAACATTCTTAACGGCACGGTTTGATGGAGTCCTTGGACTTGGATTTCAGGATATTTCTGTTGGTGAAGCCACACCAGTGTGGTTTAACATGGCGCAACAAGGTCATATGAGCCAGCAAATTTTCTCCATCTGGCTAAATCATGATCCAACATCGAAGGTGGGAGGTGAGATTGTCTTTGGTGGATTTGATTGGAGGCACTTTACTGGTGATCACACATACGTCCCAATTTCACAAAAGGGTTACTGGCAGATTGAGGTTGGAGATGTTATTGTTGCAGACACTTCTACAGGACTGTGCAAGGGCGGTTGTAGTGCCATTGTAGACTCAGGGACATCCTTCCTTGCTGGTCCAACTACTATTGTGGCTCAGATCAACCATGCCATTGGAGCAGACGGATTTGTGAGCTTGGAATGTAAAAATGTCATTTCCACTTACGGAAATTTGATATGGGAATATTTAATATCTGGGGCGCGGCCTGATATAGTGTGTGTGGACATCGGTCTATGTTCATACGATGGTTCTCGGAATATAAACAATCATATTGAGTCAGTGACCGAGAACAAAATCGGGAAGGAATCATCGGTCGATGAAACTCCTTCATGCCCCTTGTGTGAAATGATAGTCTACTGGGTTCAACTACAGCTTAAAAAACAAATAGAAAAGGAAAAAATATTTAGCTATGTGAATGAGCTGTGCGAGAAGCTTCCAAATCCGCTAAAAAGGTCATTTGTCAACTGTGATAACATTGCATTCATGCCGGATATTACATTTACGATCAGAAACAAATCCTTCACCCTCTCTCCAGAACAGTACATACTTAAAGTAGAAGAAAAATGCTCCACTGTCTGCCTTAGTGGATTTACTGCTTTGGATGTGCCTCCTCCACAAGGTCCTCTATGGGTTCTCGGAGCTCTGTTCTTGGGAGCATACCACACGGTGTTTGATTTCGGTAATCTCAGGGTAGGATTTGCAAAAGCTACGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0044238 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asp SapB_1 SapB_2

Protein Analysis

531

Amino Acids

58.64

Weight (kDa)

6.82

Isoelectric Point (pI)

36.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	105 - 529	1e-100	Eukaryotic aspartyl protease
TAXi_N	PF14543	107 - 264	8.5e-09	Xylanase inhibitor N-terminal
SapB_2	PF03489	340 - 372	1.2e-10	Saposin-like type B, region 2
SapB_1	PF05184	402 - 439	2.1e-08	Saposin-like type B, region 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002058)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G22050
fragaria_vesca	FvH4_4g21720 FvH4_4g21720 FvH4_4g21720 FvH4_6g08500 FvH4_6g08500 FvH4_6g08500 FvH4_6g08500
malus_domestica	MD04G1186500.v1.1 MD04G1186600.v1.1 MD13G1138100.v1.1 MD16G1133900.v1.1
prunus_persica	Prupe.1G103000_v2.0.a1 Prupe.1G103000_v2.0.a1 Prupe.1G103000_v2.0.a1 Prupe.1G103000_v2.0.a1 Prupe.6G304600_v2.0.a1 Prupe.6G304600_v2.0.a1 Prupe.6G304600_v2.0.a1
pyrus_communis	pycom04g16480 pycom13g12020 pycom16g11820
rosa_chinensis	RchiOBHm_Chr3g0458541 RchiOBHm_Chr4g0427921
rosa_laevigata	RLG00000007200 RLG00000025149
rosa_roxburghii	Rroxscaffold_2G00125080 Rroxscaffold_5G00369590 Rroxscaffold_6G00420990
rosa_rugosa	Rorug03G0029500 Rorug03G0029500 Rorug03G0029500 Rorug03G0029600 Rorug04G0220600
rosa_samantha	Rh3AG087900 Rh3BG091000 Rh3CG091100 Rh3DG091900 Rh4AG277600 Rh4BG283400 Rh4CG298900 Rh4DG280600
rosa_wichuraiana	Rw3G007510 Rw4G024120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	338
AccB7I	CCANNNNNTGG	1 cut(s)	390
AccI	GTMKAC	3 cut(s)	43, 930, 1233
AccII	CGCG	1 cut(s)	1081
AciI	CCGC	3 cut(s)	912, 1081, 1322
AclWI	GGATC	2 cut(s)	297, 746
AcsI	RAATTY	5 cut(s)	35, 495, 578, 726, 1048
AdeI	CACNNNGTG	1 cut(s)	1546
AfaI	GTAC	1 cut(s)	1421
AfiI	CCNNNNNNNGG	3 cut(s)	174, 390, 1496
AgsI	TTSAA	4 cut(s)	226, 405, 547, 1244
AjnI	CCWGG	1 cut(s)	242
AjuI	GAANNNNNNNTTGG	2 cut(s)	191, 223
AleI	CACNNNNGTG	3 cut(s)	26, 683, 685
AluBI	AGCT	9 cut(s)	26, 381, 1014, 1252, 1287, 1300, 1312, 1520, 1586
AluI	AGCT	9 cut(s)	26, 381, 1014, 1252, 1287, 1300, 1312, 1520, 1586
Alw21I	GWGCWC	2 cut(s)	467, 1522
AlwI	GGATC	2 cut(s)	297, 746
AoxI	GGCC	1 cut(s)	1082
ApeKI	GCWGC	5 cut(s)	75, 152, 202, 230, 378
ApoI	RAATTY	5 cut(s)	35, 495, 578, 726, 1048
AspLEI	GCGC	2 cut(s)	703, 1081
AspS9I	GGNCC	5 cut(s)	12, 211, 394, 956, 1499
AsuHPI	GGTGA	7 cut(s)	43, 208, 232, 683, 785, 827, 1393
AvaII	GGWCC	5 cut(s)	12, 211, 394, 956, 1499
BanI	GGYRCC	1 cut(s)	338
BanII	GRGCYC	1 cut(s)	1522
Bbv12I	GWGCWC	2 cut(s)	467, 1522
BbvI	GCAGC	5 cut(s)	87, 139, 214, 217, 390
BccI	CCATC	5 cut(s)	167, 406, 626, 743, 1118
BceAI	ACGGC	1 cut(s)	636
BcgI	CGANNNNNNTGC	2 cut(s)	441, 475
BciT130I	CCWGG	1 cut(s)	244
BclI	TGATCA	1 cut(s)	817
BfmI	CTRYAG	2 cut(s)	894, 1247
BisI	GCNGC	6 cut(s)	76, 153, 203, 231, 379, 1082
BlsI	GCNGC	6 cut(s)	77, 154, 204, 232, 380, 1083
Bme1390I	CCNGG	1 cut(s)	244
Bme18I	GGWCC	5 cut(s)	12, 211, 394, 956, 1499
BmgT120I	GGNCC	5 cut(s)	12, 211, 394, 956, 1499
BmiI	GGNNCC	5 cut(s)	13, 145, 340, 395, 396
BmrFI	CCNGG	1 cut(s)	244
BmrI	ACTGGG	3 cut(s)	258, 307, 1246
BmsI	GCATC	2 cut(s)	398, 420
BmuI	ACTGGG	3 cut(s)	258, 307, 1246
BoxI	GACNNNNGTC	1 cut(s)	1107
BpmI	CTGGAG	1 cut(s)	1395
BsaAI	YACGTR	1 cut(s)	1590
BsaJI	CCNNGG	3 cut(s)	70, 147, 640
BsaXI	ACNNNNNCTCC	2 cut(s)	1393, 1423
Bsc4I	CCNNNNNNNGG	3 cut(s)	174, 390, 1496
Bse118I	RCCGGY	1 cut(s)	374
Bse1I	ACTGG	7 cut(s)	168, 264, 313, 683, 817, 857, 1241
Bse3DI	GCAATG	2 cut(s)	327, 1353
BseBI	CCWGG	1 cut(s)	244
BseDI	CCNNGG	3 cut(s)	70, 147, 640
BseGI	GGATG	4 cut(s)	411, 562, 941, 1486
BseLI	CCNNNNNNNGG	3 cut(s)	174, 390, 1496
BseMI	GCAATG	2 cut(s)	327, 1353
BseMII	CTCAG	4 cut(s)	173, 948, 989, 1579
BseNI	ACTGG	7 cut(s)	168, 264, 313, 683, 817, 857, 1241
BseRI	GAGGAG	1 cut(s)	1479
BseXI	GCAGC	5 cut(s)	87, 139, 214, 217, 390
BsgI	GTGCAG	1 cut(s)	4
Bsh1236I	CGCG	1 cut(s)	1081
Bsh1285I	CGRYCG	1 cut(s)	1194
BshFI	GGCC	1 cut(s)	1084
BshNI	GGYRCC	1 cut(s)	338
BsiEI	CGRYCG	1 cut(s)	1194
BsiHKAI	GWGCWC	2 cut(s)	467, 1522
BsiSI	CCGG	3 cut(s)	375, 522, 1367
BslFI	GGGAC	6 cut(s)	120, 320, 338, 380, 815, 952
BslI	CCNNNNNNNGG	3 cut(s)	174, 390, 1496
BsmFI	GGGAC	6 cut(s)	120, 320, 338, 380, 815, 952
BsmI	GAATGC	2 cut(s)	232, 1358
BsnI	GGCC	1 cut(s)	1084
Bsp1286I	GDGCHC	2 cut(s)	467, 1522
Bsp143I	GATC	7 cut(s)	268, 289, 571, 751, 817, 978, 1383
Bsp19I	CCATGG	1 cut(s)	70
BspACI	CCGC	3 cut(s)	912, 1081, 1322
BspANI	GGCC	1 cut(s)	1084
BspCNI	CTCAG	4 cut(s)	174, 947, 988, 1578
BspFNI	CGCG	1 cut(s)	1081
BspHI	TCATGA	1 cut(s)	748
BspLI	GGNNCC	5 cut(s)	13, 145, 340, 395, 396
BspPI	GGATC	2 cut(s)	297, 746
BspT107I	GGYRCC	1 cut(s)	338
BsrDI	GCAATG	2 cut(s)	327, 1353
BsrFI	RCCGGY	1 cut(s)	374
BsrI	ACTGG	7 cut(s)	168, 264, 313, 683, 817, 857, 1241
BssAI	RCCGGY	1 cut(s)	374
BssECI	CCNNGG	3 cut(s)	70, 147, 640
BssMI	GATC	7 cut(s)	268, 289, 571, 751, 817, 978, 1383
BssT1I	CCWWGG	3 cut(s)	70, 147, 640
Bst2UI	CCWGG	1 cut(s)	244
Bst4CI	ACNGT	6 cut(s)	627, 903, 1346, 1419, 1453, 1546
BstBAI	YACGTR	1 cut(s)	1590
BstC8I	GCNNGC	2 cut(s)	454, 722
BstDEI	CTNAG	5 cut(s)	182, 934, 975, 1460, 1565
BstDSI	CCRYGG	1 cut(s)	70
BstENI	CCTNNNNNAGG	1 cut(s)	1494
BstF5I	GGATG	4 cut(s)	411, 562, 941, 1486
BstFNI	CGCG	1 cut(s)	1081
BstHHI	GCGC	2 cut(s)	703, 1081
BstKTI	GATC	7 cut(s)	271, 292, 574, 754, 820, 981, 1386
BstMBI	GATC	7 cut(s)	268, 289, 571, 751, 817, 978, 1383
BstMCI	CGRYCG	1 cut(s)	1194
BstMWI	GCNNNNNNNGC	2 cut(s)	462, 995
BstNI	CCWGG	1 cut(s)	244
BstPAI	GACNNNNGTC	1 cut(s)	1107
BstSCI	CCNGG	1 cut(s)	242
BstSFI	CTRYAG	2 cut(s)	894, 1247
BstSNI	TACGTA	1 cut(s)	1590
BstUI	CGCG	1 cut(s)	1081
BstV1I	GCAGC	5 cut(s)	87, 139, 214, 217, 390
BstX2I	RGATCY	1 cut(s)	289
BstXI	CCANNNNNNTGG	3 cut(s)	286, 320, 992
BstYI	RGATCY	1 cut(s)	289
BsuRI	GGCC	1 cut(s)	1084
BtgI	CCRYGG	1 cut(s)	70
BtsCI	GGATG	4 cut(s)	411, 562, 941, 1486
BtsIMutI	CAGTG	5 cut(s)	175, 271, 690, 1164, 1449
Cac8I	GCNNGC	2 cut(s)	454, 722
CciI	TCATGA	1 cut(s)	748
CfoI	GCGC	2 cut(s)	703, 1081
Cfr10I	RCCGGY	1 cut(s)	374
Cfr13I	GGNCC	5 cut(s)	12, 211, 394, 956, 1499
CsiI	ACCWGGT	1 cut(s)	242
Csp6I	GTAC	1 cut(s)	1420
CviAII	CATG	6 cut(s)	71, 697, 749, 986, 1209, 1363
CviQI	GTAC	1 cut(s)	1420
DdeI	CTNAG	5 cut(s)	182, 934, 975, 1460, 1565
DpnI	GATC	7 cut(s)	270, 291, 573, 753, 819, 980, 1385
DpnII	GATC	7 cut(s)	268, 289, 571, 751, 817, 978, 1383
DraIII	CACNNNGTG	1 cut(s)	1546
Ecl136II	GAGCTC	1 cut(s)	1520
Eco105I	TACGTA	1 cut(s)	1590
Eco130I	CCWWGG	3 cut(s)	70, 147, 640
Eco24I	GRGCYC	1 cut(s)	1522
Eco47I	GGWCC	5 cut(s)	12, 211, 394, 956, 1499
Eco53kI	GAGCTC	1 cut(s)	1520
EcoICRI	GAGCTC	1 cut(s)	1520
EcoNI	CCTNNNNNAGG	1 cut(s)	1494
EcoO109I	RGGNCCY	2 cut(s)	394, 1499
EcoRII	CCWGG	1 cut(s)	242
EcoT14I	CCWWGG	3 cut(s)	70, 147, 640
EcoT22I	ATGCAT	1 cut(s)	413
EcoT38I	GRGCYC	1 cut(s)	1522
ErhI	CCWWGG	3 cut(s)	70, 147, 640
FaeI	CATG	6 cut(s)	74, 700, 752, 989, 1212, 1366
FaqI	GGGAC	6 cut(s)	120, 320, 338, 380, 815, 952
FatI	CATG	6 cut(s)	70, 696, 748, 985, 1208, 1362
FauNDI	CATATG	1 cut(s)	714
FbaI	TGATCA	1 cut(s)	817
FblI	GTMKAC	3 cut(s)	43, 930, 1233
Fnu4HI	GCNGC	6 cut(s)	76, 153, 203, 231, 379, 1082
FokI	GGATG	4 cut(s)	398, 569, 928, 1493
FriOI	GRGCYC	1 cut(s)	1522
Fsp4HI	GCNGC	6 cut(s)	76, 153, 203, 231, 379, 1082
GlaI	GCGC	2 cut(s)	702, 1080
GluI	GCNGC	6 cut(s)	76, 153, 203, 231, 379, 1082
GsuI	CTGGAG	1 cut(s)	1395
HaeIII	GGCC	1 cut(s)	1084
HapII	CCGG	3 cut(s)	375, 522, 1367
HhaI	GCGC	2 cut(s)	703, 1081
Hin1II	CATG	6 cut(s)	74, 700, 752, 989, 1212, 1366
Hin6I	GCGC	2 cut(s)	701, 1079
HinP1I	GCGC	2 cut(s)	701, 1079
HincII	GTYRAC	1 cut(s)	1342
HindII	GTYRAC	1 cut(s)	1342
HindIII	AAGCTT	1 cut(s)	1310
HinfI	GANTC	6 cut(s)	237, 603, 636, 932, 1154, 1184
HpaII	CCGG	3 cut(s)	375, 522, 1367
HphI	GGTGA	7 cut(s)	43, 208, 232, 683, 785, 827, 1393
Hpy166II	GTNNAC	5 cut(s)	44, 931, 1102, 1234, 1342
Hpy188I	TCNGA	7 cut(s)	90, 294, 569, 978, 1134, 1388, 1517
Hpy188III	TCNNGA	7 cut(s)	305, 405, 575, 656, 749, 1177, 1412
Hpy8I	GTNNAC	5 cut(s)	44, 931, 1102, 1234, 1342
HpyAV	CCTTC	6 cut(s)	593, 757, 955, 1174, 1215, 1408
HpyCH4III	ACNGT	6 cut(s)	627, 903, 1346, 1419, 1453, 1546
HpyCH4IV	ACGT	2 cut(s)	828, 1589
HpyCH4V	TGCA	8 cut(s)	21, 119, 233, 411, 884, 906, 1358, 1580
HpyF10VI	GCNNNNNNNGC	2 cut(s)	462, 995
HpyF3I	CTNAG	5 cut(s)	182, 934, 975, 1460, 1565
HpySE526I	ACGT	2 cut(s)	828, 1589
Hsp92II	CATG	6 cut(s)	74, 700, 752, 989, 1212, 1366
HspAI	GCGC	2 cut(s)	701, 1079
KflI	GGGWCCC	1 cut(s)	394
Ksp22I	TGATCA	1 cut(s)	817
Kzo9I	GATC	7 cut(s)	268, 289, 571, 751, 817, 978, 1383
LmnI	GCTCC	6 cut(s)	149, 386, 995, 1451, 1517, 1532
Lsp1109I	GCAGC	5 cut(s)	87, 139, 214, 217, 390
LweI	GCATC	2 cut(s)	398, 420
MabI	ACCWGGT	1 cut(s)	242
MaeII	ACGT	2 cut(s)	828, 1589
MaeIII	GTNAC	4 cut(s)	177, 246, 848, 1159
MalI	GATC	7 cut(s)	270, 291, 573, 753, 819, 980, 1385
MboI	GATC	7 cut(s)	268, 289, 571, 751, 817, 978, 1383
MboII	GAAGA	3 cut(s)	393, 548, 1448
MflI	RGATCY	1 cut(s)	289
MhlI	GDGCHC	2 cut(s)	467, 1522
MlyI	GAGTC	3 cut(s)	645, 926, 1163
MmeI	TCCRAC	5 cut(s)	39, 303, 779, 847, 983
Mph1103I	ATGCAT	1 cut(s)	413
MseI	TTAA	6 cut(s)	102, 617, 693, 1067, 1254, 1428
MslI	CAYNNNNRTG	3 cut(s)	26, 683, 685
MspA1I	CMGCKG	1 cut(s)	26
MspI	CCGG	3 cut(s)	375, 522, 1367
MspR9I	CCNGG	1 cut(s)	244
Mva1269I	GAATGC	2 cut(s)	232, 1358
MvaI	CCWGG	1 cut(s)	244
MvnI	CGCG	1 cut(s)	1081
MwoI	GCNNNNNNNGC	2 cut(s)	462, 995
NcoI	CCATGG	1 cut(s)	70
NdeI	CATATG	1 cut(s)	714
NdeII	GATC	7 cut(s)	268, 289, 571, 751, 817, 978, 1383
NlaIII	CATG	6 cut(s)	74, 700, 752, 989, 1212, 1366
NlaIV	GGNNCC	5 cut(s)	13, 145, 340, 395, 396
NmuCI	GTSAC	2 cut(s)	246, 1159
NsiI	ATGCAT	1 cut(s)	413
OliI	CACNNNNGTG	3 cut(s)	26, 683, 685
PagI	TCATGA	1 cut(s)	748
PctI	GAATGC	2 cut(s)	232, 1358
PfeI	GAWTC	3 cut(s)	237, 603, 1184
PflMI	CCANNNNNTGG	1 cut(s)	390
PkrI	GCNGC	6 cut(s)	77, 154, 204, 232, 380, 1083
PleI	GAGTC	3 cut(s)	644, 926, 1162
PpsI	GAGTC	3 cut(s)	644, 926, 1162
Ppu21I	YACGTR	1 cut(s)	1590
PpuMI	RGGWCCY	2 cut(s)	394, 1499
PshAI	GACNNNNGTC	1 cut(s)	1107
Psp124BI	GAGCTC	1 cut(s)	1522
Psp5II	RGGWCCY	2 cut(s)	394, 1499
Psp6I	CCWGG	1 cut(s)	242
PspGI	CCWGG	1 cut(s)	242
PspN4I	GGNNCC	5 cut(s)	13, 145, 340, 395, 396
PspPI	GGNCC	5 cut(s)	12, 211, 394, 956, 1499
PspPPI	RGGWCCY	2 cut(s)	394, 1499
PsrI	GAACNNNNNNTAC	2 cut(s)	1407, 1439
PsuI	RGATCY	1 cut(s)	289
PvuII	CAGCTG	1 cut(s)	26
RsaI	GTAC	1 cut(s)	1421
RsaNI	GTAC	1 cut(s)	1420
RseI	CAYNNNNRTG	3 cut(s)	26, 683, 685
SacI	GAGCTC	1 cut(s)	1522
SaqAI	TTAA	6 cut(s)	102, 617, 693, 1067, 1254, 1428
SatI	GCNGC	6 cut(s)	76, 153, 203, 231, 379, 1082
Sau3AI	GATC	7 cut(s)	268, 289, 571, 751, 817, 978, 1383
Sau96I	GGNCC	5 cut(s)	12, 211, 394, 956, 1499
SchI	GAGTC	3 cut(s)	645, 926, 1163
ScrFI	CCNGG	1 cut(s)	244
SduI	GDGCHC	2 cut(s)	467, 1522
SexAI	ACCWGGT	1 cut(s)	242
SfaNI	GCATC	2 cut(s)	398, 420
SfcI	CTRYAG	2 cut(s)	894, 1247
SinI	GGWCC	5 cut(s)	12, 211, 394, 956, 1499
SmiMI	CAYNNNNRTG	3 cut(s)	26, 683, 685
SnaBI	TACGTA	1 cut(s)	1590
SsiI	CCGC	3 cut(s)	912, 1081, 1322
SspI	AATATT	2 cut(s)	1064, 1281
SstI	GAGCTC	1 cut(s)	1522
StyD4I	CCNGG	1 cut(s)	242
StyI	CCWWGG	3 cut(s)	70, 147, 640
TaaI	ACNGT	6 cut(s)	627, 903, 1346, 1419, 1453, 1546
TaiI	ACGT	2 cut(s)	831, 1592
TaqI	TCGA	4 cut(s)	461, 515, 761, 1194
TaqII	GACCGA	3 cut(s)	1097, 1178, 1180
TatI	WGTACW	1 cut(s)	1419
TauI	GCSGC	1 cut(s)	1084
TfiI	GAWTC	3 cut(s)	237, 603, 1184
Tru1I	TTAA	6 cut(s)	102, 617, 693, 1067, 1254, 1428
Tru9I	TTAA	6 cut(s)	102, 617, 693, 1067, 1254, 1428
TscAI	CASTG	5 cut(s)	175, 271, 690, 1164, 1456
TseFI	GTSAC	2 cut(s)	246, 1159
TseI	GCWGC	5 cut(s)	75, 152, 202, 230, 378
Tsp45I	GTSAC	2 cut(s)	246, 1159
TspDTI	ATGAA	5 cut(s)	425, 1107, 1197, 1212, 1351
TspGWI	ACGGA	2 cut(s)	1017, 1059
TspRI	CASTG	5 cut(s)	175, 271, 690, 1164, 1456
Van91I	CCANNNNNTGG	1 cut(s)	390
VpaK11BI	GGWCC	5 cut(s)	12, 211, 394, 956, 1499
XagI	CCTNNNNNAGG	1 cut(s)	1494
XapI	RAATTY	5 cut(s)	35, 495, 578, 726, 1048
XmiI	GTMKAC	3 cut(s)	43, 930, 1233
Zsp2I	ATGCAT	1 cut(s)	413

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.