Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002058

Physical Location & Seq

Genomic Coordinates Reverse (-)

27742454 .. 27746642

Gene Structure 4189 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1186600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1704 bp

ATGGAAATGCGCGTGGCCTTGCGAGTAACCAATGGTGTTGTCTCTGTATGGACCACTATAAGAAGGAAAGTGATCTCCTACCATTGTGGATTACATTTTCATCTTCTGGATGCTTCTTCTTCTTCTTGGCTCATCATCCACCATCATAGCAGCTCCACTTCCTCCAAGCTAATTGCTAATCAAGTTTCTCGAGATGGGTCTGTAAACATGCGGCACAAACATCTCTTGGTGGCTGTCTACTTATGGGCCTTAATCTCTTCACTTCTTCCTGCTTCCTCCAATGGGCTAGTCAGAATTGGTCTGAAGAAGCGACCGTTGGATTTTCAAACCATCAAAGCTGCAAGGATAGCAAGGGAGGGAAAGCCTCAGGATTTGGGTAGTTCAGACGAAGATATCGTACCTCTGAAGAATTACTTGGATGCTCAGTACTATGGGGTGATTGGAATCGGCTCACCTCCTCAGAACTTCACTGTCATATTTGACACTGGAAGCTCCAATCTTTGGGTTCCTTCTTCAAAATGTTACTTCTCTCTTGCGTGCTATTTCCACACTCGGTACAAGTCAAGCAAGTCCAGCACATATACCGCAATTGGGACGTCTGCTTCAATAACATATGGAAGTGGATCAATTTCCGGTTTCTTCAGTCAAGACAATGTTGAAGTTGGAGACCTTGTCGTCAAAAATCAAGAGTTCATTGAGGCCACAAAAGAAGGCAGTCTCACATTCGTTATAGCAAAGTTTGATGGACTTCTTGGCCTTGGATTCCAAGAAATTTCTGTTGGGAATGCTACACCACTGTGGCAAACCATGTCAGAACAAGATCTTTTAAGCGATGATGTCTTCTCGTTCTGGCTTAACCGAGACACAAATTCAGATGTAGGAGGTGAACTTGTTTTCGGCGGAGTGGACTCAAATCATTACAAAGGGAAGCATACCTATGTTCCAGTTACTAAAAAGGGTTACTGGCAGTTCGAAATGGGTGATCTTATAATCGGTAACCAGTCAACGGGTGAATGTGAGGGGGGTTGTGCTGCTATTGTGGATTCAGGAACGTCCTTGCTCACTGGCCCAACAACTACTGTGACAGTGCTTAACCATGCTATCGGAGCTGAAGGAGTAGTGAGTGCAGAATGTAAGACAGTCGTGTCTCAGTACGGAGACCTGATATGGGATCTTCTGATATCAGGGGCACAACCTGGCCAAGTTTGTAAGCAGCTTGGTTTATGTATTTTCAATGGGTCTAAGTATGTGAGCACGGGGATTGAAACAGTAGTTGAGAAGGAAGGCAAGGAGGGATCATCTGTCGGCAACGGTGCTTTGTGCACAGCTTGTGAGATGGCTGTTGTTTGGGCTCAGAATCAGCTGAAACAAAAAGGAACAAAAGAGAAAGTGCTTAGCTACATTAGTGAGCTTTGCGATAGCCTCCCAAGTCCAGCAGGAGAATCAATAGTCGAGTGCAGTAGCCTTTCGAGCATGCCAAACGTTACCTTCACCATCGGAGATAAACCTTTCGTTCTCACTCCCGACCAGTATGTTCTGAAAACTGGCGAAGGCGTCGCTGAAGTCTGTATAAGCGGGTTTACAGCTTATGATATCCCTCCTCCAACCGGTCCTCTGTGGATTCTCGGAGATGTATTCATGGGGGCGTACCACACTGTTTTCGACTTTGGTGACCTTCAAGTTGGATTTGCCGAAGCTGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0044238 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asp SapB_1 SapB_2

Protein Analysis

568

Amino Acids

61.07

Weight (kDa)

5.42

Isoelectric Point (pI)

32.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	142 - 566	1.1e-111	Eukaryotic aspartyl protease
TAXi_N	PF14543	143 - 301	2.9e-10	Xylanase inhibitor N-terminal
SapB_2	PF03489	377 - 409	9.9e-14	Saposin-like type B, region 2
SapB_1	PF05184	440 - 476	6.5e-11	Saposin-like type B, region 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002058)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G22050
fragaria_vesca	FvH4_4g21720 FvH4_4g21720 FvH4_4g21720 FvH4_6g08500 FvH4_6g08500 FvH4_6g08500 FvH4_6g08500
malus_domestica	MD04G1186500.v1.1 MD04G1186600.v1.1 MD13G1138100.v1.1 MD16G1133900.v1.1
prunus_persica	Prupe.1G103000_v2.0.a1 Prupe.1G103000_v2.0.a1 Prupe.1G103000_v2.0.a1 Prupe.1G103000_v2.0.a1 Prupe.6G304600_v2.0.a1 Prupe.6G304600_v2.0.a1 Prupe.6G304600_v2.0.a1
pyrus_communis	pycom04g16480 pycom13g12020 pycom16g11820
rosa_chinensis	RchiOBHm_Chr3g0458541 RchiOBHm_Chr4g0427921
rosa_laevigata	RLG00000007200 RLG00000025149
rosa_roxburghii	Rroxscaffold_2G00125080 Rroxscaffold_5G00369590 Rroxscaffold_6G00420990
rosa_rugosa	Rorug03G0029500 Rorug03G0029500 Rorug03G0029500 Rorug03G0029600 Rorug04G0220600
rosa_samantha	Rh3AG087900 Rh3BG091000 Rh3CG091100 Rh3DG091900 Rh4AG277600 Rh4BG283400 Rh4CG298900 Rh4DG280600
rosa_wichuraiana	Rw3G007510 Rw4G024120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	989
AasI	GACNNNNNNGTC	2 cut(s)	674, 1144
AatII	GACGTC	1 cut(s)	599
AccB7I	CCANNNNNTGG	1 cut(s)	501
AccI	GTMKAC	1 cut(s)	237
AccII	CGCG	1 cut(s)	12
AciI	CCGC	4 cut(s)	211, 585, 900, 1575
AclI	AACGTT	1 cut(s)	1482
AclWI	GGATC	3 cut(s)	631, 1179, 1303
AcoI	YGGCCR	1 cut(s)	1198
AcsI	RAATTY	2 cut(s)	771, 868
AcuI	CTGAAG	5 cut(s)	323, 425, 625, 1131, 1581
AcyI	GRCGYC	2 cut(s)	596, 1554
AfaI	GTAC	5 cut(s)	399, 428, 557, 1154, 1649
AfiI	CCNNNNNNNGG	6 cut(s)	282, 501, 591, 1006, 1168, 1607
AgeI	ACCGGT	1 cut(s)	1607
AgsI	TTSAA	7 cut(s)	326, 516, 606, 659, 1234, 1265, 1679
AjnI	CCWGG	1 cut(s)	1195
AjuI	GAANNNNNNNTTGG	6 cut(s)	299, 331, 398, 430, 762, 794
AleI	CACNNNNGTG	1 cut(s)	796
Alw21I	GWGCWC	2 cut(s)	1256, 1325
Alw26I	GTCTC	6 cut(s)	46, 660, 722, 855, 1152, 1152
Alw44I	GTGCAC	1 cut(s)	1321
AlwI	GGATC	3 cut(s)	631, 1179, 1303
Ama87I	CYCGRG	1 cut(s)	189
AoxI	GGCC	6 cut(s)	15, 246, 699, 754, 1066, 1198
ApaLI	GTGCAC	1 cut(s)	1321
ApeKI	GCWGC	5 cut(s)	150, 338, 1031, 1213, 1697
ApoI	RAATTY	2 cut(s)	771, 868
AsiGI	ACCGGT	1 cut(s)	1607
AspLEI	GCGC	1 cut(s)	12
AspS9I	GGNCC	4 cut(s)	51, 246, 1067, 1610
AsuHPI	GGTGA	7 cut(s)	444, 448, 896, 992, 1022, 1483, 1682
AsuII	TTCGAA	1 cut(s)	972
AvaI	CYCGRG	1 cut(s)	189
AvaII	GGWCC	2 cut(s)	51, 1610
AxyI	CCTNAGG	1 cut(s)	366
BaeGI	GKGCMC	2 cut(s)	1192, 1325
BalI	TGGCCA	1 cut(s)	1200
BanII	GRGCYC	1 cut(s)	1354
BarI	GAAGNNNNNNTAC	2 cut(s)	381, 413
BbsI	GAAGAC	1 cut(s)	832
Bbv12I	GWGCWC	2 cut(s)	1256, 1325
BbvI	GCAGC	5 cut(s)	162, 325, 1018, 1225, 1684
BccI	CCATC	6 cut(s)	150, 188, 338, 737, 1330, 1502
BciT130I	CCWGG	1 cut(s)	1197
BcoDI	GTCTC	6 cut(s)	46, 660, 722, 855, 1152, 1152
BfaI	CTAG	1 cut(s)	287
BglII	AGATCT	1 cut(s)	820
BisI	GCNGC	6 cut(s)	151, 212, 339, 1032, 1214, 1698
BlpI	GCTNAGC	1 cut(s)	1394
BlsI	GCNGC	6 cut(s)	152, 213, 340, 1033, 1215, 1699
BmcAI	AGTACT	1 cut(s)	428
Bme1390I	CCNGG	1 cut(s)	1197
Bme18I	GGWCC	2 cut(s)	51, 1610
BmeT110I	CYCGRG	1 cut(s)	189
BmgT120I	GGNCC	4 cut(s)	51, 246, 1067, 1610
BmiI	GGNNCC	1 cut(s)	507
BmrFI	CCNGG	1 cut(s)	1197
BmsI	GCATC	2 cut(s)	100, 409
BpiI	GAAGAC	1 cut(s)	832
Bpu1102I	GCTNAGC	1 cut(s)	1394
Bpu14I	TTCGAA	1 cut(s)	972
BsaBI	GATNNNNATC	1 cut(s)	443
BsaHI	GRCGYC	2 cut(s)	596, 1554
BsaI	GGTCTC	2 cut(s)	660, 1152
BsaJI	CCNNGG	1 cut(s)	757
BsaWI	WCCGGW	2 cut(s)	632, 1607
BsaXI	ACNNNNNCTCC	2 cut(s)	657, 687
Bsc4I	CCNNNNNNNGG	6 cut(s)	282, 501, 591, 1006, 1168, 1607
Bse118I	RCCGGY	1 cut(s)	1607
Bse1I	ACTGG	7 cut(s)	490, 944, 968, 1000, 1069, 1528, 1549
Bse21I	CCTNAGG	1 cut(s)	366
Bse8I	GATNNNNATC	1 cut(s)	443
BseBI	CCWGG	1 cut(s)	1197
BseDI	CCNNGG	1 cut(s)	757
BseGI	GGATG	3 cut(s)	115, 135, 424
BseJI	GATNNNNATC	1 cut(s)	443
BseLI	CCNNNNNNNGG	6 cut(s)	282, 501, 591, 1006, 1168, 1607
BseMII	CTCAG	5 cut(s)	380, 437, 473, 1163, 1367
BseNI	ACTGG	7 cut(s)	490, 944, 968, 1000, 1069, 1528, 1549
BseRI	GAGGAG	2 cut(s)	447, 1590
BseSI	GKGCMC	2 cut(s)	1192, 1325
BseXI	GCAGC	5 cut(s)	162, 325, 1018, 1225, 1684
BsgI	GTGCAG	2 cut(s)	1146, 1477
Bsh1236I	CGCG	1 cut(s)	12
Bsh1285I	CGRYCG	1 cut(s)	314
BshFI	GGCC	6 cut(s)	17, 248, 701, 756, 1068, 1200
BshTI	ACCGGT	1 cut(s)	1607
BsiEI	CGRYCG	1 cut(s)	314
BsiHKAI	GWGCWC	2 cut(s)	1256, 1325
BsiHKCI	CYCGRG	1 cut(s)	189
BsiSI	CCGG	2 cut(s)	633, 1608
BslFI	GGGAC	1 cut(s)	607
BslI	CCNNNNNNNGG	6 cut(s)	282, 501, 591, 1006, 1168, 1607
BsmAI	GTCTC	6 cut(s)	46, 660, 722, 855, 1152, 1152
BsmFI	GGGAC	1 cut(s)	607
BsmI	GAATGC	1 cut(s)	790
BsnI	GGCC	6 cut(s)	17, 248, 701, 756, 1068, 1200
Bso31I	GGTCTC	2 cut(s)	660, 1152
BsoBI	CYCGRG	1 cut(s)	189
Bsp119I	TTCGAA	1 cut(s)	972
Bsp1286I	GDGCHC	4 cut(s)	1192, 1256, 1325, 1354
Bsp143I	GATC	6 cut(s)	72, 623, 820, 982, 1171, 1295
Bsp1720I	GCTNAGC	1 cut(s)	1394
BspACI	CCGC	4 cut(s)	211, 585, 900, 1575
BspANI	GGCC	6 cut(s)	17, 248, 701, 756, 1068, 1200
BspCNI	CTCAG	5 cut(s)	379, 436, 472, 1162, 1366
BspFNI	CGCG	1 cut(s)	12
BspLI	GGNNCC	1 cut(s)	507
BspPI	GGATC	3 cut(s)	631, 1179, 1303
BspT104I	TTCGAA	1 cut(s)	972
BspTNI	GGTCTC	2 cut(s)	660, 1152
BsrFI	RCCGGY	1 cut(s)	1607
BsrI	ACTGG	7 cut(s)	490, 944, 968, 1000, 1069, 1528, 1549
BssAI	RCCGGY	1 cut(s)	1607
BssECI	CCNNGG	1 cut(s)	757
BssMI	GATC	6 cut(s)	72, 623, 820, 982, 1171, 1295
BssNI	GRCGYC	2 cut(s)	596, 1554
BssT1I	CCWWGG	1 cut(s)	757
Bst2UI	CCWGG	1 cut(s)	1197
Bst4CI	ACNGT	9 cut(s)	315, 472, 798, 1081, 1087, 1141, 1270, 1313, 1657
Bst6I	CTCTTC	1 cut(s)	262
BstACI	GRCGYC	2 cut(s)	596, 1554
BstBI	TTCGAA	1 cut(s)	972
BstC8I	GCNNGC	2 cut(s)	538, 1475
BstDEI	CTNAG	7 cut(s)	366, 423, 459, 1149, 1242, 1353, 1394
BstEII	GGTNACC	2 cut(s)	995, 1670
BstF5I	GGATG	3 cut(s)	115, 135, 424
BstFNI	CGCG	1 cut(s)	12
BstHHI	GCGC	1 cut(s)	12
BstKTI	GATC	6 cut(s)	75, 626, 823, 985, 1174, 1298
BstMAI	GTCTC	6 cut(s)	46, 660, 722, 855, 1152, 1152
BstMBI	GATC	6 cut(s)	72, 623, 820, 982, 1171, 1295
BstMCI	CGRYCG	1 cut(s)	314
BstMWI	GCNNNNNNNGC	5 cut(s)	347, 573, 1106, 1470, 1697
BstNI	CCWGG	1 cut(s)	1197
BstNSI	RCATGY	2 cut(s)	211, 1477
BstPI	GGTNACC	2 cut(s)	995, 1670
BstSCI	CCNGG	1 cut(s)	1195
BstSLI	GKGCMC	2 cut(s)	1192, 1325
BstUI	CGCG	1 cut(s)	12
BstV1I	GCAGC	5 cut(s)	162, 325, 1018, 1225, 1684
BstV2I	GAAGAC	1 cut(s)	832
BstX2I	RGATCY	2 cut(s)	820, 1171
BstYI	RGATCY	2 cut(s)	820, 1171
Bsu36I	CCTNAGG	1 cut(s)	366
BsuRI	GGCC	6 cut(s)	17, 248, 701, 756, 1068, 1200
BtgZI	GCGATG	1 cut(s)	846
BtsCI	GGATG	3 cut(s)	115, 135, 424
BtsIMutI	CAGTG	6 cut(s)	468, 483, 794, 1062, 1092, 1653
Cac8I	GCNNGC	2 cut(s)	538, 1475
CfoI	GCGC	1 cut(s)	12
Cfr10I	RCCGGY	1 cut(s)	1607
Cfr13I	GGNCC	4 cut(s)	51, 246, 1067, 1610
CseI	GACGC	1 cut(s)	1543
Csp6I	GTAC	5 cut(s)	398, 427, 556, 1153, 1648
CspAI	ACCGGT	1 cut(s)	1607
CviAII	CATG	5 cut(s)	208, 808, 1097, 1474, 1639
CviQI	GTAC	5 cut(s)	398, 427, 556, 1153, 1648
DdeI	CTNAG	7 cut(s)	366, 423, 459, 1149, 1242, 1353, 1394
DpnI	GATC	6 cut(s)	74, 625, 822, 984, 1173, 1297
DpnII	GATC	6 cut(s)	72, 623, 820, 982, 1171, 1295
DrdI	GACNNNNNNGTC	2 cut(s)	674, 1144
DseDI	GACNNNNNNGTC	2 cut(s)	674, 1144
EaeI	YGGCCR	1 cut(s)	1198
Eam1104I	CTCTTC	1 cut(s)	262
EarI	CTCTTC	1 cut(s)	262
EciI	GGCGGA	1 cut(s)	915
Eco130I	CCWWGG	1 cut(s)	757
Eco24I	GRGCYC	1 cut(s)	1354
Eco31I	GGTCTC	2 cut(s)	660, 1152
Eco32I	GATATC	3 cut(s)	394, 1182, 1594
Eco47I	GGWCC	2 cut(s)	51, 1610
Eco57I	CTGAAG	5 cut(s)	323, 425, 625, 1131, 1581
Eco81I	CCTNAGG	1 cut(s)	366
Eco88I	CYCGRG	1 cut(s)	189
Eco91I	GGTNACC	2 cut(s)	995, 1670
EcoO65I	GGTNACC	2 cut(s)	995, 1670
EcoRII	CCWGG	1 cut(s)	1195
EcoRV	GATATC	3 cut(s)	394, 1182, 1594
EcoT14I	CCWWGG	1 cut(s)	757
EcoT38I	GRGCYC	1 cut(s)	1354
ErhI	CCWWGG	1 cut(s)	757
FaeI	CATG	5 cut(s)	211, 811, 1100, 1477, 1642
FalI	AAGNNNNNCTT	2 cut(s)	398, 430
FaqI	GGGAC	1 cut(s)	607
FatI	CATG	5 cut(s)	207, 807, 1096, 1473, 1638
FauI	CCCGC	1 cut(s)	1568
FauNDI	CATATG	1 cut(s)	613
FblI	GTMKAC	1 cut(s)	237
Fnu4HI	GCNGC	6 cut(s)	151, 212, 339, 1032, 1214, 1698
FokI	GGATG	3 cut(s)	122, 122, 431
FriOI	GRGCYC	1 cut(s)	1354
Fsp4HI	GCNGC	6 cut(s)	151, 212, 339, 1032, 1214, 1698
FspBI	CTAG	1 cut(s)	287
GlaI	GCGC	1 cut(s)	11
GluI	GCNGC	6 cut(s)	151, 212, 339, 1032, 1214, 1698
HaeIII	GGCC	6 cut(s)	17, 248, 701, 756, 1068, 1200
HapII	CCGG	2 cut(s)	633, 1608
HgaI	GACGC	1 cut(s)	1543
HhaI	GCGC	1 cut(s)	12
Hin1I	GRCGYC	2 cut(s)	596, 1554
Hin1II	CATG	5 cut(s)	211, 811, 1100, 1477, 1642
Hin6I	GCGC	1 cut(s)	10
HinP1I	GCGC	1 cut(s)	10
HincII	GTYRAC	1 cut(s)	1005
HindII	GTYRAC	1 cut(s)	1005
HinfI	GANTC	7 cut(s)	444, 762, 908, 1043, 1357, 1442, 1621
HpaII	CCGG	2 cut(s)	633, 1608
HphI	GGTGA	7 cut(s)	444, 448, 896, 992, 1022, 1483, 1682
Hpy166II	GTNNAC	7 cut(s)	205, 238, 887, 907, 1005, 1323, 1581
Hpy188III	TCNNGA	8 cut(s)	107, 189, 191, 368, 647, 686, 1047, 1523
Hpy8I	GTNNAC	7 cut(s)	205, 238, 887, 907, 1005, 1323, 1581
Hpy99I	CGWCG	1 cut(s)	1559
HpyAV	CCTTC	9 cut(s)	57, 519, 704, 1106, 1273, 1277, 1498, 1544, 1685
HpyCH4III	ACNGT	9 cut(s)	315, 472, 798, 1081, 1087, 1141, 1270, 1313, 1657
HpyCH4IV	ACGT	3 cut(s)	596, 1052, 1482
HpyCH4V	TGCA	4 cut(s)	341, 1127, 1323, 1458
HpyF10VI	GCNNNNNNNGC	5 cut(s)	347, 573, 1106, 1470, 1697
HpyF3I	CTNAG	7 cut(s)	366, 423, 459, 1149, 1242, 1353, 1394
HpySE526I	ACGT	3 cut(s)	596, 1052, 1482
Hsp92I	GRCGYC	2 cut(s)	596, 1554
Hsp92II	CATG	5 cut(s)	211, 811, 1100, 1477, 1642
HspAI	GCGC	1 cut(s)	10
Kzo9I	GATC	6 cut(s)	72, 623, 820, 982, 1171, 1295
LmnI	GCTCC	3 cut(s)	158, 497, 1106
Lsp1109I	GCAGC	5 cut(s)	162, 325, 1018, 1225, 1684
LweI	GCATC	2 cut(s)	100, 409
MaeI	CTAG	1 cut(s)	287
MaeII	ACGT	3 cut(s)	596, 1052, 1482
MaeIII	GTNAC	8 cut(s)	25, 521, 946, 959, 995, 1081, 1483, 1670
MalI	GATC	6 cut(s)	74, 625, 822, 984, 1173, 1297
MboI	GATC	6 cut(s)	72, 623, 820, 982, 1171, 1295
MfeI	CAATTG	1 cut(s)	588
MflI	RGATCY	2 cut(s)	820, 1171
MhlI	GDGCHC	4 cut(s)	1192, 1256, 1325, 1354
MlsI	TGGCCA	1 cut(s)	1200
MluCI	AATT	7 cut(s)	171, 294, 409, 588, 627, 771, 868
MluNI	TGGCCA	1 cut(s)	1200
MlyI	GAGTC	1 cut(s)	902
MmeI	TCCRAC	4 cut(s)	297, 643, 1628, 1663
Mox20I	TGGCCA	1 cut(s)	1200
MscI	TGGCCA	1 cut(s)	1200
MseI	TTAA	4 cut(s)	251, 827, 855, 1092
MslI	CAYNNNNRTG	2 cut(s)	796, 936
Msp20I	TGGCCA	1 cut(s)	1200
MspA1I	CMGCKG	1 cut(s)	1363
MspI	CCGG	2 cut(s)	633, 1608
MspR9I	CCNGG	1 cut(s)	1197
MunI	CAATTG	1 cut(s)	588
Mva1269I	GAATGC	1 cut(s)	790
MvaI	CCWGG	1 cut(s)	1197
MvnI	CGCG	1 cut(s)	12
MwoI	GCNNNNNNNGC	5 cut(s)	347, 573, 1106, 1470, 1697
NdeI	CATATG	1 cut(s)	613
NdeII	GATC	6 cut(s)	72, 623, 820, 982, 1171, 1295
NlaIII	CATG	5 cut(s)	211, 811, 1100, 1477, 1642
NlaIV	GGNNCC	1 cut(s)	507
NmuCI	GTSAC	2 cut(s)	1081, 1670
NspI	RCATGY	2 cut(s)	211, 1477
NspV	TTCGAA	1 cut(s)	972
OliI	CACNNNNGTG	1 cut(s)	796
PaeI	GCATGC	1 cut(s)	1477
PaeR7I	CTCGAG	1 cut(s)	189
PcsI	WCGNNNNNNNCGW	1 cut(s)	393
PctI	GAATGC	1 cut(s)	790
PfeI	GAWTC	6 cut(s)	444, 762, 1043, 1357, 1442, 1621
PflFI	GACNNNGTC	1 cut(s)	671
PflMI	CCANNNNNTGG	1 cut(s)	501
PinAI	ACCGGT	1 cut(s)	1607
PkrI	GCNGC	6 cut(s)	152, 213, 340, 1033, 1215, 1699
PleI	GAGTC	1 cut(s)	902
PpsI	GAGTC	1 cut(s)	902
PsiI	TTATAA	1 cut(s)	989
Psp1406I	AACGTT	1 cut(s)	1482
Psp6I	CCWGG	1 cut(s)	1195
PspEI	GGTNACC	2 cut(s)	995, 1670
PspGI	CCWGG	1 cut(s)	1195
PspN4I	GGNNCC	1 cut(s)	507
PspPI	GGNCC	4 cut(s)	51, 246, 1067, 1610
PsuI	RGATCY	2 cut(s)	820, 1171
PsyI	GACNNNGTC	1 cut(s)	671
PvuII	CAGCTG	1 cut(s)	1363
RsaI	GTAC	5 cut(s)	399, 428, 557, 1154, 1649
RsaNI	GTAC	5 cut(s)	398, 427, 556, 1153, 1648
RseI	CAYNNNNRTG	2 cut(s)	796, 936
SaqAI	TTAA	4 cut(s)	251, 827, 855, 1092
SatI	GCNGC	6 cut(s)	151, 212, 339, 1032, 1214, 1698
Sau3AI	GATC	6 cut(s)	72, 623, 820, 982, 1171, 1295
Sau96I	GGNCC	4 cut(s)	51, 246, 1067, 1610
ScaI	AGTACT	1 cut(s)	428
SchI	GAGTC	1 cut(s)	902
ScrFI	CCNGG	1 cut(s)	1197
SduI	GDGCHC	4 cut(s)	1192, 1256, 1325, 1354
SfaNI	GCATC	2 cut(s)	100, 409
Sfr274I	CTCGAG	1 cut(s)	189
SfuI	TTCGAA	1 cut(s)	972
SinI	GGWCC	2 cut(s)	51, 1610
SlaI	CTCGAG	1 cut(s)	189
SmiMI	CAYNNNNRTG	2 cut(s)	796, 936
SmlI	CTYRAG	1 cut(s)	189
SmoI	CTYRAG	1 cut(s)	189
SphI	GCATGC	1 cut(s)	1477
Sse9I	AATT	7 cut(s)	171, 294, 409, 588, 627, 771, 868
SsiI	CCGC	4 cut(s)	211, 585, 900, 1575
SspMI	CTAG	1 cut(s)	287
StyD4I	CCNGG	1 cut(s)	1195
StyI	CCWWGG	1 cut(s)	757
TaaI	ACNGT	9 cut(s)	315, 472, 798, 1081, 1087, 1141, 1270, 1313, 1657
TaiI	ACGT	3 cut(s)	599, 1055, 1485
TaqI	TCGA	5 cut(s)	190, 972, 1452, 1469, 1662
TasI	AATT	7 cut(s)	171, 294, 409, 588, 627, 771, 868
TatI	WGTACW	1 cut(s)	426
TauI	GCSGC	1 cut(s)	214
TfiI	GAWTC	6 cut(s)	444, 762, 1043, 1357, 1442, 1621
Tru1I	TTAA	4 cut(s)	251, 827, 855, 1092
Tru9I	TTAA	4 cut(s)	251, 827, 855, 1092
TscAI	CASTG	6 cut(s)	475, 490, 801, 1069, 1092, 1660
TseFI	GTSAC	2 cut(s)	1081, 1670
TseI	GCWGC	5 cut(s)	150, 338, 1031, 1213, 1697
Tsp45I	GTSAC	2 cut(s)	1081, 1670
TspDTI	ATGAA	3 cut(s)	89, 682, 1627
TspGWI	ACGGA	1 cut(s)	1170
TspRI	CASTG	6 cut(s)	475, 490, 801, 1069, 1092, 1660
Tth111I	GACNNNGTC	1 cut(s)	671
Van91I	CCANNNNNTGG	1 cut(s)	501
VneI	GTGCAC	1 cut(s)	1321
VpaK11BI	GGWCC	2 cut(s)	51, 1610
XapI	RAATTY	2 cut(s)	771, 868
XceI	RCATGY	2 cut(s)	211, 1477
XhoI	CTCGAG	1 cut(s)	189
XmiI	GTMKAC	1 cut(s)	237
XspI	CTAG	1 cut(s)	287
ZraI	GACGTC	1 cut(s)	597
ZrmI	AGTACT	1 cut(s)	428

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.