Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002058

Physical Location & Seq

Genomic Coordinates Reverse (-)

10626956 .. 10630824

Gene Structure 3869 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1138100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1500 bp

ATGGGGTTGAAGGTACTTCTGGTTGCAATTTGCCTGTGGGTTTGGTCAGCCTCTTTGTCTAGTGCTACAAAATCCAGTGATGGGCTACTGAGAATTGGTCTAAAGAAGCAGCCTTTGGACCTCACCAGATTAAATGATGCAAGACTCACCAGGTCACAAGCTCTTCAACCCAATGATCTTAAATCCAACATTGTGTATCTGAAAAATTATCTGGACACCCAGTTTTATGGAGAGATTGCTATTGGCACCCCACCACAGTACTTGGGTGTTGTCTTTGACACTGGCAGCTCCAATCTGTGGGTCCCATCTTCAAGATGCATCTTTTCTATTTCTTGCTATTTTCATTCTAAATACAGGGCAAGCCTTTCAAGCACTTACTCCAAAATTGGAATACCCTGCAAAATTCCTTATGGGTCTGGTTCGATTTCCGGCTACTTCAGCAGAGATAATTCGAAAATTGGGAATGTTATCATCAAAGATCAAGAATTTGTTGAGATTACAAGGGAAGGATTCCTAACATTCTTAACGGCGAAGTTTGATGGAGTCCTTGGACTTGGATTTCAGGATATCTCTGTTGGTGAAGCCACACCAGTGTGGTTTAACATGGTGCAACAAGGTCATATGAGCCAGGAAATTTTCTCCATTTGGCTAAATCATGATCCAACATCGAAGTTGGGAGGTGAGATCGTCTTTGGTGGATTTGATTGGAGGCACTTTATTGGTGATCACACCTATGTCCCAATTTCACAAAAGGGTTACTGGCAGATTGAGGTTGGAGATGTTATCGTTGCAGACAATTCTACAGGACTGTGCAAGGGCGGGTGTGGTGCCATTATAGATTCAGGGACATCCTTCCTCGCTGGTCCAACTACTATTGTGGCTCAGATCAACCATGCCATTGGAGCAGACGGGCTTGCGAGCTTGGAATGTAAAAATGTCATTTCCACTTATGGAAATTTGATATGGGAATATTTAGTATCTGGGGTACGGCCTGATATAGTGTGTGTGGACATGGGTCTATGTTTATACGACAGTTCTCGGAATATAAACAATAACATTGAGTCAGTGATTGAGAGCAAAACCGGGAGGGAGTCATCAGTCGATGAAACTCCTTTATGTACCTTGTGTGAAATGGTAGTCTACTGGGTTCAACTACAGCTTAAAAAACAAATAGCAAAGGAAAAAATATTTAGCTATGTGAATGAGTTGTGCGAGAAGCTTCCAAATCCGGTAAAAAAGTCGTTTGTCAACTGTGATAACATTGCGTTCATGCCAGACATTTCATTTACAATTGGAAACAAATCCTTCGCCCTCTCTCCAGAACAGTACATACTTAAAGTTGAAGAAAAATGCTCCACTGTCTGCCTTAGTGGATTTACTGCTTTGGATGTGCCTCCTCCACAAGGTCCTCTATGGGTTCTTGGAGCTCTGTTCTTGGGAGCATACCACACGGTGTTTGACTTCGGTAATCTCAGGGTAGGATTCGCAAAAGCTACGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0044238 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asp SapB_1 SapB_2

Protein Analysis

500

Amino Acids

54.87

Weight (kDa)

5.87

Isoelectric Point (pI)

36.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	74 - 498	4.1e-100	Eukaryotic aspartyl protease
SapB_2	PF03489	309 - 341	1.5e-10	Saposin-like type B, region 2
SapB_1	PF05184	371 - 408	2.9e-09	Saposin-like type B, region 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002058)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G22050
fragaria_vesca	FvH4_4g21720 FvH4_4g21720 FvH4_4g21720 FvH4_6g08500 FvH4_6g08500 FvH4_6g08500 FvH4_6g08500
malus_domestica	MD04G1186500.v1.1 MD04G1186600.v1.1 MD13G1138100.v1.1 MD16G1133900.v1.1
prunus_persica	Prupe.1G103000_v2.0.a1 Prupe.1G103000_v2.0.a1 Prupe.1G103000_v2.0.a1 Prupe.1G103000_v2.0.a1 Prupe.6G304600_v2.0.a1 Prupe.6G304600_v2.0.a1 Prupe.6G304600_v2.0.a1
pyrus_communis	pycom04g16480 pycom13g12020 pycom16g11820
rosa_chinensis	RchiOBHm_Chr3g0458541 RchiOBHm_Chr4g0427921
rosa_laevigata	RLG00000007200 RLG00000025149
rosa_roxburghii	Rroxscaffold_2G00125080 Rroxscaffold_5G00369590 Rroxscaffold_6G00420990
rosa_rugosa	Rorug03G0029500 Rorug03G0029500 Rorug03G0029500 Rorug03G0029600 Rorug04G0220600
rosa_samantha	Rh3AG087900 Rh3BG091000 Rh3CG091100 Rh3DG091900 Rh4AG277600 Rh4BG283400 Rh4CG298900 Rh4DG280600
rosa_wichuraiana	Rw3G007510 Rw4G024120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	245, 827
AccB7I	CCANNNNNTGG	1 cut(s)	297
AccI	GTMKAC	1 cut(s)	1140
AciI	CCGC	1 cut(s)	819
AclWI	GGATC	1 cut(s)	653
AcsI	RAATTY	4 cut(s)	402, 485, 633, 955
AcuI	CTGAAG	1 cut(s)	421
AdeI	CACNNNGTG	1 cut(s)	1453
AfaI	GTAC	5 cut(s)	15, 260, 987, 1120, 1328
AfiI	CCNNNNNNNGG	3 cut(s)	81, 297, 1403
AgsI	TTSAA	6 cut(s)	10, 167, 312, 369, 1151, 1343
AjnI	CCWGG	2 cut(s)	149, 627
AjuI	GAANNNNNNNTTGG	2 cut(s)	98, 130
AleI	CACNNNNGTG	2 cut(s)	590, 592
AluBI	AGCT	8 cut(s)	161, 288, 921, 1159, 1194, 1219, 1427, 1493
AluI	AGCT	8 cut(s)	161, 288, 921, 1159, 1194, 1219, 1427, 1493
Alw21I	GWGCWC	1 cut(s)	1429
AlwI	GGATC	1 cut(s)	653
AoxI	GGCC	1 cut(s)	989
ApeKI	GCWGC	2 cut(s)	109, 285
ApoI	RAATTY	4 cut(s)	402, 485, 633, 955
AspS9I	GGNCC	4 cut(s)	118, 301, 863, 1406
AsuC2I	CCSGG	1 cut(s)	1084
AsuHPI	GGTGA	5 cut(s)	115, 139, 590, 692, 734
AsuII	TTCGAA	1 cut(s)	452
AvaII	GGWCC	4 cut(s)	118, 301, 863, 1406
BanI	GGYRCC	2 cut(s)	245, 827
BanII	GRGCYC	1 cut(s)	1429
Bbv12I	GWGCWC	1 cut(s)	1429
BbvI	GCAGC	2 cut(s)	121, 297
BccI	CCATC	3 cut(s)	74, 313, 533
BceAI	ACGGC	2 cut(s)	543, 1004
BciT130I	CCWGG	2 cut(s)	151, 629
BclI	TGATCA	1 cut(s)	724
BcnI	CCSGG	1 cut(s)	1084
BfaI	CTAG	1 cut(s)	60
BfmI	CTRYAG	2 cut(s)	801, 1154
BisI	GCNGC	2 cut(s)	110, 286
BlsI	GCNGC	2 cut(s)	111, 287
BmcAI	AGTACT	1 cut(s)	260
Bme1390I	CCNGG	3 cut(s)	151, 629, 1084
Bme18I	GGWCC	4 cut(s)	118, 301, 863, 1406
BmgT120I	GGNCC	4 cut(s)	118, 301, 863, 1406
BmiI	GGNNCC	4 cut(s)	247, 302, 303, 829
BmrFI	CCNGG	3 cut(s)	151, 629, 1084
BmrI	ACTGGG	2 cut(s)	214, 1153
BmsI	GCATC	3 cut(s)	127, 305, 327
BmuI	ACTGGG	2 cut(s)	214, 1153
BoxI	GACNNNNGTC	1 cut(s)	1014
BpmI	CTGGAG	1 cut(s)	1302
Bpu14I	TTCGAA	1 cut(s)	452
BpuMI	CCSGG	1 cut(s)	1084
BsaAI	YACGTR	1 cut(s)	1497
BsaJI	CCNNGG	1 cut(s)	547
BsaWI	WCCGGW	1 cut(s)	1228
Bsc4I	CCNNNNNNNGG	3 cut(s)	81, 297, 1403
Bse1I	ACTGG	6 cut(s)	75, 220, 286, 590, 764, 1148
Bse3DI	GCAATG	1 cut(s)	1260
BseBI	CCWGG	2 cut(s)	151, 629
BseDI	CCNNGG	1 cut(s)	547
BseGI	GGATG	2 cut(s)	848, 1393
BseLI	CCNNNNNNNGG	3 cut(s)	81, 297, 1403
BseMI	GCAATG	1 cut(s)	1260
BseMII	CTCAG	3 cut(s)	80, 896, 1486
BseNI	ACTGG	6 cut(s)	75, 220, 286, 590, 764, 1148
BseRI	GAGGAG	1 cut(s)	1386
BseXI	GCAGC	2 cut(s)	121, 297
BshFI	GGCC	1 cut(s)	991
BshNI	GGYRCC	2 cut(s)	245, 827
BsiHKAI	GWGCWC	1 cut(s)	1429
BsiSI	CCGG	3 cut(s)	429, 1083, 1229
BslFI	GGGAC	3 cut(s)	287, 722, 859
BslI	CCNNNNNNNGG	3 cut(s)	81, 297, 1403
BsmFI	GGGAC	3 cut(s)	287, 722, 859
BsnI	GGCC	1 cut(s)	991
Bsp119I	TTCGAA	1 cut(s)	452
Bsp1286I	GDGCHC	1 cut(s)	1429
Bsp143I	GATC	6 cut(s)	175, 478, 658, 684, 724, 885
BspACI	CCGC	1 cut(s)	819
BspANI	GGCC	1 cut(s)	991
BspCNI	CTCAG	3 cut(s)	81, 895, 1485
BspHI	TCATGA	1 cut(s)	655
BspLI	GGNNCC	4 cut(s)	247, 302, 303, 829
BspPI	GGATC	1 cut(s)	653
BspQI	GCTCTTC	1 cut(s)	168
BspT104I	TTCGAA	1 cut(s)	452
BspT107I	GGYRCC	2 cut(s)	245, 827
BsrDI	GCAATG	1 cut(s)	1260
BsrI	ACTGG	6 cut(s)	75, 220, 286, 590, 764, 1148
BssECI	CCNNGG	1 cut(s)	547
BssMI	GATC	6 cut(s)	175, 478, 658, 684, 724, 885
BssT1I	CCWWGG	1 cut(s)	547
Bst2UI	CCWGG	2 cut(s)	151, 629
Bst4CI	ACNGT	7 cut(s)	258, 810, 1034, 1253, 1326, 1360, 1453
Bst6I	CTCTTC	1 cut(s)	168
BstBAI	YACGTR	1 cut(s)	1497
BstBI	TTCGAA	1 cut(s)	452
BstC8I	GCNNGC	3 cut(s)	361, 915, 919
BstDEI	CTNAG	4 cut(s)	89, 882, 1367, 1472
BstENI	CCTNNNNNAGG	1 cut(s)	1401
BstF5I	GGATG	2 cut(s)	848, 1393
BstKTI	GATC	6 cut(s)	178, 481, 661, 687, 727, 888
BstMBI	GATC	6 cut(s)	175, 478, 658, 684, 724, 885
BstMWI	GCNNNNNNNGC	3 cut(s)	369, 438, 902
BstNI	CCWGG	2 cut(s)	151, 629
BstPAI	GACNNNNGTC	1 cut(s)	1014
BstSCI	CCNGG	3 cut(s)	149, 627, 1082
BstSFI	CTRYAG	2 cut(s)	801, 1154
BstSNI	TACGTA	1 cut(s)	1497
BstV1I	GCAGC	2 cut(s)	121, 297
BstXI	CCANNNNNNTGG	2 cut(s)	227, 899
BsuRI	GGCC	1 cut(s)	991
BtsCI	GGATG	2 cut(s)	848, 1393
BtsIMutI	CAGTG	5 cut(s)	82, 279, 597, 1071, 1356
Cac8I	GCNNGC	3 cut(s)	361, 915, 919
CciI	TCATGA	1 cut(s)	655
Cfr13I	GGNCC	4 cut(s)	118, 301, 863, 1406
CsiI	ACCWGGT	1 cut(s)	149
Csp6I	GTAC	5 cut(s)	14, 259, 986, 1119, 1327
CspCI	CAANNNNNGTGG	2 cut(s)	243, 278
CviAII	CATG	5 cut(s)	604, 656, 893, 1012, 1270
CviQI	GTAC	5 cut(s)	14, 259, 986, 1119, 1327
DdeI	CTNAG	4 cut(s)	89, 882, 1367, 1472
DpnI	GATC	6 cut(s)	177, 480, 660, 686, 726, 887
DpnII	GATC	6 cut(s)	175, 478, 658, 684, 724, 885
DraIII	CACNNNGTG	1 cut(s)	1453
Eam1104I	CTCTTC	1 cut(s)	168
EarI	CTCTTC	1 cut(s)	168
Ecl136II	GAGCTC	1 cut(s)	1427
Eco105I	TACGTA	1 cut(s)	1497
Eco130I	CCWWGG	1 cut(s)	547
Eco24I	GRGCYC	1 cut(s)	1429
Eco32I	GATATC	1 cut(s)	568
Eco47I	GGWCC	4 cut(s)	118, 301, 863, 1406
Eco53kI	GAGCTC	1 cut(s)	1427
Eco57I	CTGAAG	1 cut(s)	421
EcoICRI	GAGCTC	1 cut(s)	1427
EcoNI	CCTNNNNNAGG	1 cut(s)	1401
EcoO109I	RGGNCCY	2 cut(s)	301, 1406
EcoRII	CCWGG	2 cut(s)	149, 627
EcoRV	GATATC	1 cut(s)	568
EcoT14I	CCWWGG	1 cut(s)	547
EcoT22I	ATGCAT	1 cut(s)	320
EcoT38I	GRGCYC	1 cut(s)	1429
ErhI	CCWWGG	1 cut(s)	547
FaeI	CATG	5 cut(s)	607, 659, 896, 1015, 1273
FaqI	GGGAC	3 cut(s)	287, 722, 859
FatI	CATG	5 cut(s)	603, 655, 892, 1011, 1269
FauI	CCCGC	1 cut(s)	812
FauNDI	CATATG	1 cut(s)	621
FbaI	TGATCA	1 cut(s)	724
FblI	GTMKAC	1 cut(s)	1140
Fnu4HI	GCNGC	2 cut(s)	110, 286
FokI	GGATG	2 cut(s)	835, 1400
FriOI	GRGCYC	1 cut(s)	1429
Fsp4HI	GCNGC	2 cut(s)	110, 286
FspBI	CTAG	1 cut(s)	60
GluI	GCNGC	2 cut(s)	110, 286
GsuI	CTGGAG	1 cut(s)	1302
HaeIII	GGCC	1 cut(s)	991
HapII	CCGG	3 cut(s)	429, 1083, 1229
Hin1II	CATG	5 cut(s)	607, 659, 896, 1015, 1273
HincII	GTYRAC	1 cut(s)	1249
HindII	GTYRAC	1 cut(s)	1249
HindIII	AAGCTT	1 cut(s)	1217
HinfI	GANTC	7 cut(s)	144, 510, 543, 839, 1061, 1091, 1482
HpaII	CCGG	3 cut(s)	429, 1083, 1229
HphI	GGTGA	5 cut(s)	115, 139, 590, 692, 734
Hpy166II	GTNNAC	3 cut(s)	1009, 1141, 1249
Hpy188I	TCNGA	3 cut(s)	201, 885, 1041
Hpy188III	TCNNGA	6 cut(s)	212, 312, 482, 563, 656, 1319
Hpy8I	GTNNAC	3 cut(s)	1009, 1141, 1249
HpyAV	CCTTC	4 cut(s)	4, 500, 862, 1315
HpyCH4III	ACNGT	7 cut(s)	258, 810, 1034, 1253, 1326, 1360, 1453
HpyCH4IV	ACGT	1 cut(s)	1496
HpyCH4V	TGCA	7 cut(s)	26, 140, 318, 399, 610, 791, 813
HpyF10VI	GCNNNNNNNGC	3 cut(s)	369, 438, 902
HpyF3I	CTNAG	4 cut(s)	89, 882, 1367, 1472
HpySE526I	ACGT	1 cut(s)	1496
Hsp92II	CATG	5 cut(s)	607, 659, 896, 1015, 1273
KflI	GGGWCCC	1 cut(s)	301
Ksp22I	TGATCA	1 cut(s)	724
Kzo9I	GATC	6 cut(s)	175, 478, 658, 684, 724, 885
LguI	GCTCTTC	1 cut(s)	168
LmnI	GCTCC	5 cut(s)	293, 902, 1358, 1424, 1439
Lsp1109I	GCAGC	2 cut(s)	121, 297
LweI	GCATC	3 cut(s)	127, 305, 327
MabI	ACCWGGT	1 cut(s)	149
MaeI	CTAG	1 cut(s)	60
MaeII	ACGT	1 cut(s)	1496
MaeIII	GTNAC	2 cut(s)	153, 755
MalI	GATC	6 cut(s)	177, 480, 660, 686, 726, 887
MboI	GATC	6 cut(s)	175, 478, 658, 684, 724, 885
MboII	GAAGA	3 cut(s)	155, 300, 1355
MfeI	CAATTG	1 cut(s)	1290
MhlI	GDGCHC	1 cut(s)	1429
MlyI	GAGTC	4 cut(s)	138, 552, 1070, 1100
MmeI	TCCRAC	4 cut(s)	210, 686, 754, 890
Mph1103I	ATGCAT	1 cut(s)	320
MseI	TTAA	6 cut(s)	131, 180, 524, 600, 1161, 1335
MslI	CAYNNNNRTG	3 cut(s)	590, 592, 732
MspI	CCGG	3 cut(s)	429, 1083, 1229
MspR9I	CCNGG	3 cut(s)	151, 629, 1084
MunI	CAATTG	1 cut(s)	1290
MvaI	CCWGG	2 cut(s)	151, 629
MwoI	GCNNNNNNNGC	3 cut(s)	369, 438, 902
NciI	CCSGG	1 cut(s)	1084
NdeI	CATATG	1 cut(s)	621
NdeII	GATC	6 cut(s)	175, 478, 658, 684, 724, 885
NlaIII	CATG	5 cut(s)	607, 659, 896, 1015, 1273
NlaIV	GGNNCC	4 cut(s)	247, 302, 303, 829
NmuCI	GTSAC	1 cut(s)	153
NsiI	ATGCAT	1 cut(s)	320
NspV	TTCGAA	1 cut(s)	452
OliI	CACNNNNGTG	2 cut(s)	590, 592
PagI	TCATGA	1 cut(s)	655
PciSI	GCTCTTC	1 cut(s)	168
PfeI	GAWTC	3 cut(s)	510, 839, 1482
PflMI	CCANNNNNTGG	1 cut(s)	297
PkrI	GCNGC	2 cut(s)	111, 287
PleI	GAGTC	4 cut(s)	138, 551, 1069, 1099
PpsI	GAGTC	4 cut(s)	138, 551, 1069, 1099
Ppu21I	YACGTR	1 cut(s)	1497
PpuMI	RGGWCCY	2 cut(s)	301, 1406
PshAI	GACNNNNGTC	1 cut(s)	1014
Psp124BI	GAGCTC	1 cut(s)	1429
Psp5II	RGGWCCY	2 cut(s)	301, 1406
Psp6I	CCWGG	2 cut(s)	149, 627
PspGI	CCWGG	2 cut(s)	149, 627
PspN4I	GGNNCC	4 cut(s)	247, 302, 303, 829
PspPI	GGNCC	4 cut(s)	118, 301, 863, 1406
PspPPI	RGGWCCY	2 cut(s)	301, 1406
PsrI	GAACNNNNNNTAC	2 cut(s)	1314, 1346
RsaI	GTAC	5 cut(s)	15, 260, 987, 1120, 1328
RsaNI	GTAC	5 cut(s)	14, 259, 986, 1119, 1327
RseI	CAYNNNNRTG	3 cut(s)	590, 592, 732
SacI	GAGCTC	1 cut(s)	1429
SapI	GCTCTTC	1 cut(s)	168
SaqAI	TTAA	6 cut(s)	131, 180, 524, 600, 1161, 1335
SatI	GCNGC	2 cut(s)	110, 286
Sau3AI	GATC	6 cut(s)	175, 478, 658, 684, 724, 885
Sau96I	GGNCC	4 cut(s)	118, 301, 863, 1406
ScaI	AGTACT	1 cut(s)	260
SchI	GAGTC	4 cut(s)	138, 552, 1070, 1100
ScrFI	CCNGG	3 cut(s)	151, 629, 1084
SduI	GDGCHC	1 cut(s)	1429
SexAI	ACCWGGT	1 cut(s)	149
SfaNI	GCATC	3 cut(s)	127, 305, 327
SfcI	CTRYAG	2 cut(s)	801, 1154
SfuI	TTCGAA	1 cut(s)	452
SinI	GGWCC	4 cut(s)	118, 301, 863, 1406
SmiMI	CAYNNNNRTG	3 cut(s)	590, 592, 732
SnaBI	TACGTA	1 cut(s)	1497
SsiI	CCGC	1 cut(s)	819
SspI	AATATT	2 cut(s)	971, 1188
SspMI	CTAG	1 cut(s)	60
SstI	GAGCTC	1 cut(s)	1429
StyD4I	CCNGG	3 cut(s)	149, 627, 1082
StyI	CCWWGG	1 cut(s)	547
TaaI	ACNGT	7 cut(s)	258, 810, 1034, 1253, 1326, 1360, 1453
TaiI	ACGT	1 cut(s)	1499
TaqI	TCGA	4 cut(s)	422, 452, 668, 1101
TatI	WGTACW	2 cut(s)	258, 1326
TfiI	GAWTC	3 cut(s)	510, 839, 1482
Tru1I	TTAA	6 cut(s)	131, 180, 524, 600, 1161, 1335
Tru9I	TTAA	6 cut(s)	131, 180, 524, 600, 1161, 1335
TscAI	CASTG	5 cut(s)	82, 286, 597, 1071, 1363
TseFI	GTSAC	1 cut(s)	153
TseI	GCWGC	2 cut(s)	109, 285
Tsp45I	GTSAC	1 cut(s)	153
TspDTI	ATGAA	4 cut(s)	332, 1119, 1258, 1272
TspRI	CASTG	5 cut(s)	82, 286, 597, 1071, 1363
Van91I	CCANNNNNTGG	1 cut(s)	297
VpaK11BI	GGWCC	4 cut(s)	118, 301, 863, 1406
XagI	CCTNNNNNAGG	1 cut(s)	1401
XapI	RAATTY	4 cut(s)	402, 485, 633, 955
XmiI	GTMKAC	1 cut(s)	1140
XspI	CTAG	1 cut(s)	60
ZrmI	AGTACT	1 cut(s)	260
Zsp2I	ATGCAT	1 cut(s)	320

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.