Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002058

Physical Location & Seq

Genomic Coordinates Reverse (-)

27727313 .. 27731185

Gene Structure 3873 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1186500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1398 bp

ATGGATATGTCTGTAGACATGCGGCACAAACATCTCTTGGTGGCTCTCTACTTATGGGCCTTAATCTCTTCACTTCTTCCTGCTTCCTCCAATGGCCTAGTCAGAATTGGTCTGAAGAAGCGACCGTTGGATCTTCAAACCATCAAAGCTGGGACTGCAAGGGAGGGAAAGCCTCGGGATTTGGGTAGTTCAGATGAAGATATCGTACCTCTGAAGAATTACTTGGATGCTCAACACTATGGGGTGATTGGAATTGGCTCACCTCCTCAGAACTTCACTGTCATATTTGACACTGGAAGCTCCAATCTTTGGGTTCCTTCTTCAAAATGTTACTTCTCTCTTGCGTGCTATTTCCACACTCGGTACAAGTCAAGCAAGTCCAGCACACATACCGCAATTGGGACGTCTGCTTCAATAACATATGGAAGCGGATCAATTTCTGGTTTCTTCAGTCAAGATAATGTTGAAGTTGGAGACCTTGTTGTAAAAAATAAAGATTTCATTGAGGCCACAAAAGAAGACAGTCTCACATTCGTTATAGCAAAGTTTGATGGACTTCTTGGCCTTGGATTCCAAGAAATTTCTGTTGGGAATGCTACACCAGTGTGTGGCGCTGATTATAGTCTTTTGAACGATGATGTCTTCTCGTTCTGGCTTAACCGAGACACAACTTCAGATGTAGGAGGTGAACTTGTTTTCAATGGAGTGGACTCAAATCATTACAAAGGGAAGCATACCTATGTTCCAGTTACCAAAAAGGGTGTATGTGAGGGGGGTTGTGCTGCTATTGTGGATTCAGGAACGTCCTTGCTCACCGGCCCAACAGTATTAGTGAGTGCAGAATGTAAGGCAGTCGTGTCTCAGTACGGAGACCTGATATGGGATCTTCTGATCTCAGGGGCACATCCTGGCCAAGTTTGTAAGCAGCTTGGTTTATGTGTTTTCAATGGGGCTCAGTATGTTAGCACCGGGATTGAAACAGTGGTTGAGAAGGAAAGCAAGGAGGGATCAGCTGTCGGCGACAGTGCTTTGTGCACAGCTTGTGAGATGGCTGTTGTCTGGGCTCAGAATCAGCTGAAACAGAAAGGAACAAAAGAGAAAGTACTTAGCTACATTAGTGAGCTTTGTGAGAGCCTCCCAAGTCCAGCAGGAGAATCAATAGTCGAGTGCAATAGCCTTTCAAGCATGCCAAACGTTGCCTTCACCATCGGAGATAAACCTTTCGTTCTCACTCCGGACCAGTATGTTCTGAAAACTGGTGAAGGCGTCGCTGAAGTCTCTATCAGCGGGTTTACAGCTTACGATGTCGCTCCTCCAACAGGTCCTCTCTGGTACTTTATCATTAACCTTAAACGATTATCCAGCTTCGATGCATTTTCATTAGGGTTTAGGTCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0044238 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asp SapB_1 SapB_2

Protein Analysis

466

Amino Acids

49.84

Weight (kDa)

5.27

Isoelectric Point (pI)

34.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	79 - 444	5.8e-73	Eukaryotic aspartyl protease
TAXi_N	PF14543	83 - 233	5.1e-09	Xylanase inhibitor N-terminal
SapB_2	PF03489	281 - 313	7e-13	Saposin-like type B, region 2
SapB_1	PF05184	344 - 380	1e-10	Saposin-like type B, region 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002058)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G22050
fragaria_vesca	FvH4_4g21720 FvH4_4g21720 FvH4_4g21720 FvH4_6g08500 FvH4_6g08500 FvH4_6g08500 FvH4_6g08500
malus_domestica	MD04G1186500.v1.1 MD04G1186600.v1.1 MD13G1138100.v1.1 MD16G1133900.v1.1
prunus_persica	Prupe.1G103000_v2.0.a1 Prupe.1G103000_v2.0.a1 Prupe.1G103000_v2.0.a1 Prupe.1G103000_v2.0.a1 Prupe.6G304600_v2.0.a1 Prupe.6G304600_v2.0.a1 Prupe.6G304600_v2.0.a1
pyrus_communis	pycom04g16480 pycom13g12020 pycom16g11820
rosa_chinensis	RchiOBHm_Chr3g0458541 RchiOBHm_Chr4g0427921
rosa_laevigata	RLG00000007200 RLG00000025149
rosa_roxburghii	Rroxscaffold_2G00125080 Rroxscaffold_5G00369590 Rroxscaffold_6G00420990
rosa_rugosa	Rorug03G0029500 Rorug03G0029500 Rorug03G0029500 Rorug03G0029600 Rorug04G0220600
rosa_samantha	Rh3AG087900 Rh3BG091000 Rh3CG091100 Rh3DG091900 Rh4AG277600 Rh4BG283400 Rh4CG298900 Rh4DG280600
rosa_wichuraiana	Rw3G007510 Rw4G024120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	407
AccB7I	CCANNNNNTGG	2 cut(s)	309, 608
AccI	GTMKAC	1 cut(s)	15
AccIII	TCCGGA	1 cut(s)	1234
AciI	CCGC	4 cut(s)	22, 393, 429, 1287
AclI	AACGTT	1 cut(s)	1194
AclWI	GGATC	4 cut(s)	138, 439, 891, 1015
AcoI	YGGCCR	1 cut(s)	910
AcsI	RAATTY	1 cut(s)	579
AcuI	CTGAAG	5 cut(s)	134, 233, 433, 657, 1293
AcyI	GRCGYC	2 cut(s)	404, 1266
AfaI	GTAC	5 cut(s)	207, 365, 866, 1104, 1334
AfiI	CCNNNNNNNGG	5 cut(s)	309, 399, 608, 880, 1319
AgsI	TTSAA	9 cut(s)	137, 324, 414, 467, 631, 700, 946, 977, 1182
AjnI	CCWGG	1 cut(s)	907
AjuI	GAANNNNNNNTTGG	6 cut(s)	110, 142, 206, 238, 570, 602
AleI	CACNNNNGTG	1 cut(s)	604
Alw21I	GWGCWC	1 cut(s)	1037
Alw26I	GTCTC	6 cut(s)	468, 530, 657, 864, 864, 1282
Alw44I	GTGCAC	1 cut(s)	1033
AlwI	GGATC	4 cut(s)	138, 439, 891, 1015
Ama87I	CYCGRG	1 cut(s)	174
Aor13HI	TCCGGA	1 cut(s)	1234
AoxI	GGCC	6 cut(s)	57, 94, 507, 562, 817, 910
ApaLI	GTGCAC	1 cut(s)	1033
ApeKI	GCWGC	2 cut(s)	782, 925
ApoI	RAATTY	1 cut(s)	579
AspLEI	GCGC	1 cut(s)	614
AspS9I	GGNCC	5 cut(s)	57, 818, 1237, 1322, 1392
AsuC2I	CCSGG	1 cut(s)	970
AsuHPI	GGTGA	6 cut(s)	252, 256, 698, 805, 1195, 1271
AvaI	CYCGRG	1 cut(s)	174
AvaII	GGWCC	3 cut(s)	1237, 1322, 1392
BaeGI	GKGCMC	2 cut(s)	904, 1037
BalI	TGGCCA	1 cut(s)	912
BanII	GRGCYC	2 cut(s)	955, 1066
BarI	GAAGNNNNNNTAC	2 cut(s)	189, 221
BbsI	GAAGAC	2 cut(s)	525, 634
Bbv12I	GWGCWC	1 cut(s)	1037
BbvI	GCAGC	2 cut(s)	769, 937
BccI	CCATC	4 cut(s)	149, 545, 1042, 1214
BciT130I	CCWGG	1 cut(s)	909
BcnI	CCSGG	1 cut(s)	970
BcoDI	GTCTC	6 cut(s)	468, 530, 657, 864, 864, 1282
BfaI	CTAG	2 cut(s)	98, 1396
BfmI	CTRYAG	1 cut(s)	12
BfoI	RGCGCY	1 cut(s)	615
BisI	GCNGC	3 cut(s)	23, 783, 926
BlsI	GCNGC	3 cut(s)	24, 784, 927
BmcAI	AGTACT	1 cut(s)	1104
Bme1390I	CCNGG	2 cut(s)	909, 970
Bme18I	GGWCC	3 cut(s)	1237, 1322, 1392
BmeT110I	CYCGRG	1 cut(s)	174
BmgT120I	GGNCC	5 cut(s)	57, 818, 1237, 1322, 1392
BmiI	GGNNCC	1 cut(s)	315
BmrFI	CCNGG	2 cut(s)	909, 970
BmsI	GCATC	2 cut(s)	217, 1360
BpiI	GAAGAC	2 cut(s)	525, 634
BpuMI	CCSGG	1 cut(s)	970
BsaHI	GRCGYC	2 cut(s)	404, 1266
BsaI	GGTCTC	2 cut(s)	468, 864
BsaJI	CCNNGG	2 cut(s)	173, 565
BsaWI	WCCGGW	1 cut(s)	1234
BsaXI	ACNNNNNCTCC	2 cut(s)	465, 495
Bsc4I	CCNNNNNNNGG	5 cut(s)	309, 399, 608, 880, 1319
Bse118I	RCCGGY	1 cut(s)	815
Bse1I	ACTGG	5 cut(s)	298, 602, 746, 1240, 1261
BseAI	TCCGGA	1 cut(s)	1234
BseBI	CCWGG	1 cut(s)	909
BseDI	CCNNGG	2 cut(s)	173, 565
BseGI	GGATG	2 cut(s)	232, 904
BseLI	CCNNNNNNNGG	5 cut(s)	309, 399, 608, 880, 1319
BseMII	CTCAG	5 cut(s)	281, 875, 909, 968, 1079
BseNI	ACTGG	5 cut(s)	298, 602, 746, 1240, 1261
BseRI	GAGGAG	2 cut(s)	255, 1302
BseSI	GKGCMC	2 cut(s)	904, 1037
BseXI	GCAGC	2 cut(s)	769, 937
BseYI	CCCAGC	1 cut(s)	149
BsgI	GTGCAG	1 cut(s)	858
Bsh1285I	CGRYCG	1 cut(s)	125
BshFI	GGCC	6 cut(s)	59, 96, 509, 564, 819, 912
BsiEI	CGRYCG	1 cut(s)	125
BsiHKAI	GWGCWC	1 cut(s)	1037
BsiHKCI	CYCGRG	1 cut(s)	174
BsiSI	CCGG	3 cut(s)	816, 969, 1235
BslFI	GGGAC	2 cut(s)	166, 415
BslI	CCNNNNNNNGG	5 cut(s)	309, 399, 608, 880, 1319
BsmAI	GTCTC	6 cut(s)	468, 530, 657, 864, 864, 1282
BsmFI	GGGAC	2 cut(s)	166, 415
BsmI	GAATGC	1 cut(s)	598
BsnI	GGCC	6 cut(s)	59, 96, 509, 564, 819, 912
Bso31I	GGTCTC	2 cut(s)	468, 864
BsoBI	CYCGRG	1 cut(s)	174
Bsp1286I	GDGCHC	4 cut(s)	904, 955, 1037, 1066
Bsp13I	TCCGGA	1 cut(s)	1234
Bsp143I	GATC	5 cut(s)	130, 431, 883, 891, 1007
BspACI	CCGC	4 cut(s)	22, 393, 429, 1287
BspANI	GGCC	6 cut(s)	59, 96, 509, 564, 819, 912
BspCNI	CTCAG	5 cut(s)	280, 874, 908, 967, 1078
BspEI	TCCGGA	1 cut(s)	1234
BspLI	GGNNCC	1 cut(s)	315
BspPI	GGATC	4 cut(s)	138, 439, 891, 1015
BspTNI	GGTCTC	2 cut(s)	468, 864
BsrFI	RCCGGY	1 cut(s)	815
BsrI	ACTGG	5 cut(s)	298, 602, 746, 1240, 1261
BssAI	RCCGGY	1 cut(s)	815
BssECI	CCNNGG	2 cut(s)	173, 565
BssMI	GATC	5 cut(s)	130, 431, 883, 891, 1007
BssNI	GRCGYC	2 cut(s)	404, 1266
BssT1I	CCWWGG	1 cut(s)	565
Bst2UI	CCWGG	1 cut(s)	909
Bst4CI	ACNGT	6 cut(s)	126, 280, 524, 826, 982, 1025
Bst6I	CTCTTC	1 cut(s)	73
BstACI	GRCGYC	2 cut(s)	404, 1266
BstC8I	GCNNGC	2 cut(s)	346, 1187
BstDEI	CTNAG	6 cut(s)	267, 861, 895, 954, 1065, 1106
BstENI	CCTNNNNNAGG	1 cut(s)	1317
BstF5I	GGATG	2 cut(s)	232, 904
BstH2I	RGCGCY	1 cut(s)	615
BstHHI	GCGC	1 cut(s)	614
BstKTI	GATC	5 cut(s)	133, 434, 886, 894, 1010
BstMAI	GTCTC	6 cut(s)	468, 530, 657, 864, 864, 1282
BstMBI	GATC	5 cut(s)	130, 431, 883, 891, 1007
BstMCI	CGRYCG	1 cut(s)	125
BstMWI	GCNNNNNNNGC	3 cut(s)	155, 381, 1182
BstNI	CCWGG	1 cut(s)	909
BstNSI	RCATGY	2 cut(s)	22, 1189
BstSCI	CCNGG	2 cut(s)	907, 968
BstSFI	CTRYAG	1 cut(s)	12
BstSLI	GKGCMC	2 cut(s)	904, 1037
BstV1I	GCAGC	2 cut(s)	769, 937
BstV2I	GAAGAC	2 cut(s)	525, 634
BstX2I	RGATCY	2 cut(s)	130, 883
BstYI	RGATCY	2 cut(s)	130, 883
BsuRI	GGCC	6 cut(s)	59, 96, 509, 564, 819, 912
BtsCI	GGATG	2 cut(s)	232, 904
BtsIMutI	CAGTG	5 cut(s)	276, 291, 609, 987, 1030
Cac8I	GCNNGC	2 cut(s)	346, 1187
CfoI	GCGC	1 cut(s)	614
Cfr10I	RCCGGY	1 cut(s)	815
Cfr13I	GGNCC	5 cut(s)	57, 818, 1237, 1322, 1392
CseI	GACGC	1 cut(s)	1255
Csp6I	GTAC	5 cut(s)	206, 364, 865, 1103, 1333
CviAII	CATG	2 cut(s)	19, 1186
CviQI	GTAC	5 cut(s)	206, 364, 865, 1103, 1333
DdeI	CTNAG	6 cut(s)	267, 861, 895, 954, 1065, 1106
DpnI	GATC	5 cut(s)	132, 433, 885, 893, 1009
DpnII	GATC	5 cut(s)	130, 431, 883, 891, 1007
EaeI	YGGCCR	1 cut(s)	910
Eam1104I	CTCTTC	1 cut(s)	73
EarI	CTCTTC	1 cut(s)	73
Eco130I	CCWWGG	1 cut(s)	565
Eco24I	GRGCYC	2 cut(s)	955, 1066
Eco31I	GGTCTC	2 cut(s)	468, 864
Eco32I	GATATC	1 cut(s)	202
Eco47I	GGWCC	3 cut(s)	1237, 1322, 1392
Eco57I	CTGAAG	5 cut(s)	134, 233, 433, 657, 1293
Eco88I	CYCGRG	1 cut(s)	174
EcoNI	CCTNNNNNAGG	1 cut(s)	1317
EcoO109I	RGGNCCY	2 cut(s)	1322, 1392
EcoRII	CCWGG	1 cut(s)	907
EcoRV	GATATC	1 cut(s)	202
EcoT14I	CCWWGG	1 cut(s)	565
EcoT22I	ATGCAT	1 cut(s)	1375
EcoT38I	GRGCYC	2 cut(s)	955, 1066
ErhI	CCWWGG	1 cut(s)	565
FaeI	CATG	2 cut(s)	22, 1189
FalI	AAGNNNNNCTT	2 cut(s)	206, 238
FaqI	GGGAC	2 cut(s)	166, 415
FatI	CATG	2 cut(s)	18, 1185
FauI	CCCGC	1 cut(s)	1280
FauNDI	CATATG	1 cut(s)	421
FblI	GTMKAC	1 cut(s)	15
Fnu4HI	GCNGC	3 cut(s)	23, 783, 926
FokI	GGATG	2 cut(s)	239, 891
FriOI	GRGCYC	2 cut(s)	955, 1066
Fsp4HI	GCNGC	3 cut(s)	23, 783, 926
FspBI	CTAG	2 cut(s)	98, 1396
GlaI	GCGC	1 cut(s)	613
GluI	GCNGC	3 cut(s)	23, 783, 926
GsaI	CCCAGC	1 cut(s)	153
HaeII	RGCGCY	1 cut(s)	615
HaeIII	GGCC	6 cut(s)	59, 96, 509, 564, 819, 912
HapII	CCGG	3 cut(s)	816, 969, 1235
HgaI	GACGC	1 cut(s)	1255
HhaI	GCGC	1 cut(s)	614
Hin1I	GRCGYC	2 cut(s)	404, 1266
Hin1II	CATG	2 cut(s)	22, 1189
Hin6I	GCGC	1 cut(s)	612
HinP1I	GCGC	1 cut(s)	612
HinfI	GANTC	5 cut(s)	570, 710, 794, 1069, 1154
HpaII	CCGG	3 cut(s)	816, 969, 1235
HphI	GGTGA	6 cut(s)	252, 256, 698, 805, 1195, 1271
Hpy166II	GTNNAC	5 cut(s)	16, 689, 709, 1035, 1293
Hpy188III	TCNNGA	4 cut(s)	176, 455, 798, 1235
Hpy8I	GTNNAC	5 cut(s)	16, 689, 709, 1035, 1293
Hpy99I	CGWCG	1 cut(s)	1271
HpyAV	CCTTC	4 cut(s)	327, 985, 1210, 1256
HpyCH4III	ACNGT	6 cut(s)	126, 280, 524, 826, 982, 1025
HpyCH4IV	ACGT	3 cut(s)	404, 803, 1194
HpyCH4V	TGCA	5 cut(s)	158, 839, 1035, 1170, 1373
HpyF10VI	GCNNNNNNNGC	3 cut(s)	155, 381, 1182
HpyF3I	CTNAG	6 cut(s)	267, 861, 895, 954, 1065, 1106
HpySE526I	ACGT	3 cut(s)	404, 803, 1194
Hsp92I	GRCGYC	2 cut(s)	404, 1266
Hsp92II	CATG	2 cut(s)	22, 1189
HspAI	GCGC	1 cut(s)	612
Kpn2I	TCCGGA	1 cut(s)	1234
Kzo9I	GATC	5 cut(s)	130, 431, 883, 891, 1007
LmnI	GCTCC	2 cut(s)	305, 1315
Lsp1109I	GCAGC	2 cut(s)	769, 937
LweI	GCATC	2 cut(s)	217, 1360
MaeI	CTAG	2 cut(s)	98, 1396
MaeII	ACGT	3 cut(s)	404, 803, 1194
MaeIII	GTNAC	2 cut(s)	329, 748
MalI	GATC	5 cut(s)	132, 433, 885, 893, 1009
MboI	GATC	5 cut(s)	130, 431, 883, 891, 1007
MfeI	CAATTG	1 cut(s)	396
MflI	RGATCY	2 cut(s)	130, 883
MhlI	GDGCHC	4 cut(s)	904, 955, 1037, 1066
MlsI	TGGCCA	1 cut(s)	912
MluCI	AATT	6 cut(s)	105, 217, 252, 396, 435, 579
MluNI	TGGCCA	1 cut(s)	912
MlyI	GAGTC	1 cut(s)	704
MmeI	TCCRAC	3 cut(s)	108, 451, 1340
Mox20I	TGGCCA	1 cut(s)	912
Mph1103I	ATGCAT	1 cut(s)	1375
MroI	TCCGGA	1 cut(s)	1234
MscI	TGGCCA	1 cut(s)	912
MseI	TTAA	4 cut(s)	62, 657, 1344, 1350
MslI	CAYNNNNRTG	2 cut(s)	604, 738
Msp20I	TGGCCA	1 cut(s)	912
MspA1I	CMGCKG	3 cut(s)	1013, 1075, 1287
MspI	CCGG	3 cut(s)	816, 969, 1235
MspR9I	CCNGG	2 cut(s)	909, 970
MunI	CAATTG	1 cut(s)	396
Mva1269I	GAATGC	1 cut(s)	598
MvaI	CCWGG	1 cut(s)	909
MwoI	GCNNNNNNNGC	3 cut(s)	155, 381, 1182
NciI	CCSGG	1 cut(s)	970
NdeI	CATATG	1 cut(s)	421
NdeII	GATC	5 cut(s)	130, 431, 883, 891, 1007
NlaIII	CATG	2 cut(s)	22, 1189
NlaIV	GGNNCC	1 cut(s)	315
NsiI	ATGCAT	1 cut(s)	1375
NspI	RCATGY	2 cut(s)	22, 1189
OliI	CACNNNNGTG	1 cut(s)	604
PaeI	GCATGC	1 cut(s)	1189
PctI	GAATGC	1 cut(s)	598
PfeI	GAWTC	4 cut(s)	570, 794, 1069, 1154
PflMI	CCANNNNNTGG	2 cut(s)	309, 608
PkrI	GCNGC	3 cut(s)	24, 784, 927
PleI	GAGTC	1 cut(s)	704
PpsI	GAGTC	1 cut(s)	704
PpuMI	RGGWCCY	2 cut(s)	1322, 1392
Psp1406I	AACGTT	1 cut(s)	1194
Psp5II	RGGWCCY	2 cut(s)	1322, 1392
Psp6I	CCWGG	1 cut(s)	907
PspFI	CCCAGC	1 cut(s)	149
PspGI	CCWGG	1 cut(s)	907
PspN4I	GGNNCC	1 cut(s)	315
PspPI	GGNCC	5 cut(s)	57, 818, 1237, 1322, 1392
PspPPI	RGGWCCY	2 cut(s)	1322, 1392
PsuI	RGATCY	2 cut(s)	130, 883
PvuII	CAGCTG	2 cut(s)	1013, 1075
RsaI	GTAC	5 cut(s)	207, 365, 866, 1104, 1334
RsaNI	GTAC	5 cut(s)	206, 364, 865, 1103, 1333
RseI	CAYNNNNRTG	2 cut(s)	604, 738
SaqAI	TTAA	4 cut(s)	62, 657, 1344, 1350
SatI	GCNGC	3 cut(s)	23, 783, 926
Sau3AI	GATC	5 cut(s)	130, 431, 883, 891, 1007
Sau96I	GGNCC	5 cut(s)	57, 818, 1237, 1322, 1392
ScaI	AGTACT	1 cut(s)	1104
SchI	GAGTC	1 cut(s)	704
ScrFI	CCNGG	2 cut(s)	909, 970
SduI	GDGCHC	4 cut(s)	904, 955, 1037, 1066
SfaNI	GCATC	2 cut(s)	217, 1360
SfcI	CTRYAG	1 cut(s)	12
SinI	GGWCC	3 cut(s)	1237, 1322, 1392
SmiMI	CAYNNNNRTG	2 cut(s)	604, 738
SphI	GCATGC	1 cut(s)	1189
Sse9I	AATT	6 cut(s)	105, 217, 252, 396, 435, 579
SsiI	CCGC	4 cut(s)	22, 393, 429, 1287
SspMI	CTAG	2 cut(s)	98, 1396
StyD4I	CCNGG	2 cut(s)	907, 968
StyI	CCWWGG	1 cut(s)	565
TaaI	ACNGT	6 cut(s)	126, 280, 524, 826, 982, 1025
TaiI	ACGT	3 cut(s)	407, 806, 1197
TaqI	TCGA	2 cut(s)	1164, 1368
TasI	AATT	6 cut(s)	105, 217, 252, 396, 435, 579
TatI	WGTACW	1 cut(s)	1102
TauI	GCSGC	1 cut(s)	25
TfiI	GAWTC	4 cut(s)	570, 794, 1069, 1154
Tru1I	TTAA	4 cut(s)	62, 657, 1344, 1350
Tru9I	TTAA	4 cut(s)	62, 657, 1344, 1350
TscAI	CASTG	5 cut(s)	283, 298, 609, 987, 1030
TseI	GCWGC	2 cut(s)	782, 925
TspDTI	ATGAA	3 cut(s)	210, 490, 1368
TspGWI	ACGGA	1 cut(s)	882
TspRI	CASTG	5 cut(s)	283, 298, 609, 987, 1030
Van91I	CCANNNNNTGG	2 cut(s)	309, 608
VneI	GTGCAC	1 cut(s)	1033
VpaK11BI	GGWCC	3 cut(s)	1237, 1322, 1392
XagI	CCTNNNNNAGG	1 cut(s)	1317
XapI	RAATTY	1 cut(s)	579
XceI	RCATGY	2 cut(s)	22, 1189
XmiI	GTMKAC	1 cut(s)	15
XspI	CTAG	2 cut(s)	98, 1396
ZraI	GACGTC	1 cut(s)	405
ZrmI	AGTACT	1 cut(s)	1104
Zsp2I	ATGCAT	1 cut(s)	1375

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.