Rroxscaffold_6G00420990

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002058
Physical Location & Seq
Reverse (-)
42187280 .. 42190269
2990 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00420990.1

Sequence Viewer

Length: 1227 bp
ATGTCGAGTAAATATCTCTTGGTGGCTTTGTGCTTATGGACTTTAATCTGTGCACTGCTTCCTGCTTCCTCTAATGGCCTAGTGAGAATCGGTCTGAAGAAGCGAACCTTGGATCTTCAGACCATCAAAGCTGCAAGAGCAGCAAGGGAGGCAAAATTTAGAGGGACTACTATAAGGGGCGTGATTGATCATAACTATTTGGGTGATGATGAAGATATAGTTCCCCTGGAGAATTATATGGATGCTCAATACTATGGTGAGATTGGAAAGTCTGCTCAAATAACATACGGAAGTGGATCCATTTCTGGTTTTTTCAGTCAAGATAATGTTCTAGTCGGCGATGTAGTTGTCAAAAATCAGGCAGGCTTTTTCTTTAACTTGTCTACAATGGCAGAGCAAGGTCTTTTAGATGATGAGGTCTTCTCATTCTGGCTTAATCGAGATGCAGATGCAGAAGTTGGAGGTGAACTTGTTTTTGGTGGTGTTGACAAAACGCACTATAAGGGAAAGCATACCTATGTTCCAGTTACTAAAAAGGGCTACTGGCAGGTGTCTGTGAGGGGGTTGTGCCGCTATCGTGGATTCAGGAACGTCCTTACTCACTGGCCCAACGTTAACCATGCTATTGGAGCTGAAGGAGTAGTGAGTACAGAATGTAAGCAAATTGTTTCTCAGTATGGAGACTTGATATGGAATCTTCTAATATCAGGGGTACAACCTGACCAAGTATGTAAGCAGCTTGGTTTATGTGTTTTGAATGGGGGCCAGTCTGTGAGTGCTGGAATTGAAACAGTGATCGAAAGAGAAGATAGGGAGGAATCATCTGTTGGTGACACTGCTTTGTGCACAGCTTGTGAGATGGCTGTTGTTTGGGCCCAGAATCAGCTGAGACAAAAAGGAACAAAAGAAAGAGTACTTCAGTACATTGATGAGCTGTGTGAGAGCCTGCCAAGTCCAATGGGAGAGTCGATAATTGACTGCAATAGCATTTCAAGCATGCCGAACATTACCTTCACCATCGGAAATCAGCTTTTTGTTCTCACTCCACAACAGTATGTTCTGAAAACTGGCCAAGGCACTACTGGTATCTGCATCAGTGGGTTTACAGCTTTCGATATACCCCCTCCAACGGGTCCTCTCTGGATCCTTGGAGATGTATTTATGGGGGCATATCACACAATTTTCGACTTTGGTAACCTCGAGATCGGATTTGCCGAAGCTGCTTAA

Protein Analysis

408

Amino Acids

44.68

Weight (kDa)

5.04

Isoelectric Point (pI)

33.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 86 - 121 8.5e-06 Eukaryotic aspartyl protease
Asp PF00026 127 - 188 6e-17 Eukaryotic aspartyl protease
SapB_2 PF03489 217 - 250 7e-15 Saposin-like type B, region 2
SapB_1 PF05184 281 - 317 5.6e-11 Saposin-like type B, region 1
Asp PF00026 302 - 407 2.4e-22 Eukaryotic aspartyl protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 538
Acc36I ACCTGC 1 cut(s) 538
AccI GTMKAC 1 cut(s) 383
AciI CCGC 1 cut(s) 571
AclI AACGTT 1 cut(s) 612
AclWI GGATC 5 cut(s) 120, 291, 304, 1138, 1151
AcoI YGGCCR 1 cut(s) 1069
AcsI RAATTY 1 cut(s) 155
AcuI CTGAAG 4 cut(s) 101, 116, 654, 902
AfaI GTAC 4 cut(s) 649, 714, 915, 923
AfiI CCNNNNNNNGG 2 cut(s) 1129, 1130
AgsI TTSAA 3 cut(s) 757, 788, 993
AjnI CCWGG 1 cut(s) 225
AjuI GAANNNNNNNTTGG 6 cut(s) 92, 124, 459, 491, 810, 842
AluBI AGCT 9 cut(s) 131, 632, 739, 851, 886, 934, 1030, 1109, 1220
AluI AGCT 9 cut(s) 131, 632, 739, 851, 886, 934, 1030, 1109, 1220
Alw21I GWGCWC 2 cut(s) 55, 848
Alw26I GTCTC 2 cut(s) 675, 883
Alw44I GTGCAC 2 cut(s) 51, 844
AlwI GGATC 5 cut(s) 120, 291, 304, 1138, 1151
Ama87I CYCGRG 1 cut(s) 1199
AoxI GGCC 5 cut(s) 76, 605, 763, 873, 1069
ApaI GGGCCC 1 cut(s) 877
ApaLI GTGCAC 2 cut(s) 51, 844
ApeKI GCWGC 4 cut(s) 131, 140, 736, 1220
ApoI RAATTY 1 cut(s) 155
AspS9I GGNCC 5 cut(s) 606, 763, 873, 874, 1133
AsuHPI GGTGA 5 cut(s) 215, 269, 476, 842, 1006
AvaI CYCGRG 1 cut(s) 1199
AvaII GGWCC 1 cut(s) 1133
BaeGI GKGCMC 3 cut(s) 55, 848, 877
BalI TGGCCA 1 cut(s) 1071
BamHI GGATCC 2 cut(s) 296, 1143
BanII GRGCYC 1 cut(s) 877
BbsI GAAGAC 1 cut(s) 412
Bbv12I GWGCWC 2 cut(s) 55, 848
BbvI GCAGC 4 cut(s) 118, 152, 748, 1207
BccI CCATC 3 cut(s) 131, 853, 1025
BciT130I CCWGG 1 cut(s) 227
BclI TGATCA 1 cut(s) 187
BcoDI GTCTC 2 cut(s) 675, 883
BfaI CTAG 2 cut(s) 80, 332
BfuAI ACCTGC 1 cut(s) 538
BisI GCNGC 5 cut(s) 132, 141, 571, 737, 1221
BlsI GCNGC 5 cut(s) 133, 142, 572, 738, 1222
BmcAI AGTACT 1 cut(s) 915
Bme1390I CCNGG 1 cut(s) 227
Bme18I GGWCC 1 cut(s) 1133
BmeT110I CYCGRG 1 cut(s) 1199
BmgT120I GGNCC 5 cut(s) 606, 763, 873, 874, 1133
BmiI GGNNCC 5 cut(s) 298, 764, 875, 1134, 1145
BmrFI CCNGG 1 cut(s) 227
BmsI GCATC 4 cut(s) 232, 433, 439, 1101
BpiI GAAGAC 1 cut(s) 412
BplI GAGNNNNNCTC 2 cut(s) 407, 439
BpmI CTGGAG 1 cut(s) 248
BsaJI CCNNGG 4 cut(s) 108, 225, 1072, 1147
Bsc4I CCNNNNNNNGG 2 cut(s) 1129, 1130
Bse1I ACTGG 6 cut(s) 524, 548, 608, 766, 1072, 1087
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 4 cut(s) 108, 225, 1072, 1147
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 2 cut(s) 1129, 1130
BseMII CTCAG 2 cut(s) 686, 878
BseNI ACTGG 6 cut(s) 524, 548, 608, 766, 1072, 1087
BseSI GKGCMC 3 cut(s) 55, 848, 877
BseXI GCAGC 4 cut(s) 118, 152, 748, 1207
BshFI GGCC 5 cut(s) 78, 607, 765, 875, 1071
BsiHKAI GWGCWC 2 cut(s) 55, 848
BsiHKCI CYCGRG 1 cut(s) 1199
BslFI GGGAC 1 cut(s) 178
BslI CCNNNNNNNGG 2 cut(s) 1129, 1130
BsmAI GTCTC 2 cut(s) 675, 883
BsmFI GGGAC 1 cut(s) 178
BsnI GGCC 5 cut(s) 78, 607, 765, 875, 1071
BsoBI CYCGRG 1 cut(s) 1199
Bsp120I GGGCCC 1 cut(s) 873
Bsp1286I GDGCHC 3 cut(s) 55, 848, 877
Bsp143I GATC 6 cut(s) 112, 187, 296, 795, 1143, 1203
BspACI CCGC 1 cut(s) 571
BspANI GGCC 5 cut(s) 78, 607, 765, 875, 1071
BspCNI CTCAG 2 cut(s) 685, 879
BspLI GGNNCC 5 cut(s) 298, 764, 875, 1134, 1145
BspMI ACCTGC 1 cut(s) 538
BspPI GGATC 5 cut(s) 120, 291, 304, 1138, 1151
BsrI ACTGG 6 cut(s) 524, 548, 608, 766, 1072, 1087
BssECI CCNNGG 4 cut(s) 108, 225, 1072, 1147
BssMI GATC 6 cut(s) 112, 187, 296, 795, 1143, 1203
BssT1I CCWWGG 3 cut(s) 108, 1072, 1147
Bst2UI CCWGG 1 cut(s) 227
Bst4CI ACNGT 2 cut(s) 793, 1053
BstC8I GCNNGC 3 cut(s) 364, 947, 998
BstDEI CTNAG 2 cut(s) 672, 887
BstEII GGTNACC 1 cut(s) 1193
BstF5I GGATG 1 cut(s) 247
BstKTI GATC 6 cut(s) 115, 190, 299, 798, 1146, 1206
BstMAI GTCTC 2 cut(s) 675, 883
BstMBI GATC 6 cut(s) 112, 187, 296, 795, 1143, 1203
BstMWI GCNNNNNNNGC 6 cut(s) 137, 140, 149, 629, 993, 1220
BstNI CCWGG 1 cut(s) 227
BstNSI RCATGY 1 cut(s) 1000
BstPI GGTNACC 1 cut(s) 1193
BstSCI CCNGG 1 cut(s) 225
BstSLI GKGCMC 3 cut(s) 55, 848, 877
BstV1I GCAGC 4 cut(s) 118, 152, 748, 1207
BstV2I GAAGAC 1 cut(s) 412
BstX2I RGATCY 3 cut(s) 112, 296, 1143
BstXI CCANNNNNNTGG 1 cut(s) 626
BstYI RGATCY 3 cut(s) 112, 296, 1143
BsuRI GGCC 5 cut(s) 78, 607, 765, 875, 1071
BtgZI GCGATG 1 cut(s) 354
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 2 cut(s) 53, 834
BtsIMutI CAGTG 5 cut(s) 53, 601, 798, 834, 1102
BveI ACCTGC 1 cut(s) 538
Cac8I GCNNGC 3 cut(s) 364, 947, 998
Cfr13I GGNCC 5 cut(s) 606, 763, 873, 874, 1133
Csp6I GTAC 4 cut(s) 648, 713, 914, 922
CviAII CATG 2 cut(s) 620, 997
CviQI GTAC 4 cut(s) 648, 713, 914, 922
DdeI CTNAG 2 cut(s) 672, 887
DpnI GATC 6 cut(s) 114, 189, 298, 797, 1145, 1205
DpnII GATC 6 cut(s) 112, 187, 296, 795, 1143, 1203
EaeI YGGCCR 1 cut(s) 1069
Eco130I CCWWGG 3 cut(s) 108, 1072, 1147
Eco24I GRGCYC 1 cut(s) 877
Eco47I GGWCC 1 cut(s) 1133
Eco57I CTGAAG 4 cut(s) 101, 116, 654, 902
Eco88I CYCGRG 1 cut(s) 1199
Eco91I GGTNACC 1 cut(s) 1193
EcoO109I RGGNCCY 1 cut(s) 1133
EcoO65I GGTNACC 1 cut(s) 1193
EcoRII CCWGG 1 cut(s) 225
EcoT14I CCWWGG 3 cut(s) 108, 1072, 1147
EcoT38I GRGCYC 1 cut(s) 877
ErhI CCWWGG 3 cut(s) 108, 1072, 1147
FaeI CATG 2 cut(s) 623, 1000
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FaqI GGGAC 1 cut(s) 178
FatI CATG 2 cut(s) 619, 996
FbaI TGATCA 1 cut(s) 187
FblI GTMKAC 1 cut(s) 383
Fnu4HI GCNGC 5 cut(s) 132, 141, 571, 737, 1221
FokI GGATG 1 cut(s) 254
FriOI GRGCYC 1 cut(s) 877
Fsp4HI GCNGC 5 cut(s) 132, 141, 571, 737, 1221
FspBI CTAG 2 cut(s) 80, 332
GluI GCNGC 5 cut(s) 132, 141, 571, 737, 1221
GsuI CTGGAG 1 cut(s) 248
HaeIII GGCC 5 cut(s) 78, 607, 765, 875, 1071
Hin1II CATG 2 cut(s) 623, 1000
HincII GTYRAC 2 cut(s) 487, 616
HindII GTYRAC 2 cut(s) 487, 616
HinfI GANTC 6 cut(s) 87, 582, 694, 818, 880, 965
HpaI GTTAAC 1 cut(s) 616
HphI GGTGA 5 cut(s) 215, 269, 476, 842, 1006
Hpy166II GTNNAC 7 cut(s) 53, 384, 467, 487, 616, 846, 1104
Hpy188I TCNGA 5 cut(s) 96, 120, 1022, 1062, 1208
Hpy188III TCNNGA 5 cut(s) 320, 440, 586, 1141, 1201
Hpy8I GTNNAC 7 cut(s) 53, 384, 467, 487, 616, 846, 1104
HpyAV CCTTC 2 cut(s) 629, 1021
HpyCH4III ACNGT 2 cut(s) 793, 1053
HpyCH4IV ACGT 2 cut(s) 591, 612
HpyCH4V TGCA 7 cut(s) 53, 134, 446, 452, 846, 981, 1092
HpyF10VI GCNNNNNNNGC 6 cut(s) 137, 140, 149, 629, 993, 1220
HpyF3I CTNAG 2 cut(s) 672, 887
HpySE526I ACGT 2 cut(s) 591, 612
Hsp92II CATG 2 cut(s) 623, 1000
Ksp22I TGATCA 1 cut(s) 187
KspAI GTTAAC 1 cut(s) 616
Kzo9I GATC 6 cut(s) 112, 187, 296, 795, 1143, 1203
LmnI GCTCC 1 cut(s) 629
Lsp1109I GCAGC 4 cut(s) 118, 152, 748, 1207
LweI GCATC 4 cut(s) 232, 433, 439, 1101
MaeI CTAG 2 cut(s) 80, 332
MaeII ACGT 2 cut(s) 591, 612
MaeIII GTNAC 3 cut(s) 526, 830, 1193
MalI GATC 6 cut(s) 114, 189, 298, 797, 1145, 1205
MboI GATC 6 cut(s) 112, 187, 296, 795, 1143, 1203
MboII GAAGA 6 cut(s) 107, 109, 224, 412, 689, 818
MflI RGATCY 3 cut(s) 112, 296, 1143
MhlI GDGCHC 3 cut(s) 55, 848, 877
MlsI TGGCCA 1 cut(s) 1071
MluCI AATT 6 cut(s) 155, 232, 663, 783, 972, 1179
MluNI TGGCCA 1 cut(s) 1071
MlyI GAGTC 1 cut(s) 974
MmeI TCCRAC 2 cut(s) 439, 1151
Mox20I TGGCCA 1 cut(s) 1071
MscI TGGCCA 1 cut(s) 1071
MseI TTAA 5 cut(s) 44, 375, 435, 615, 1225
MslI CAYNNNNRTG 1 cut(s) 516
Msp20I TGGCCA 1 cut(s) 1071
MspA1I CMGCKG 1 cut(s) 886
MspR9I CCNGG 1 cut(s) 227
MvaI CCWGG 1 cut(s) 227
MwoI GCNNNNNNNGC 6 cut(s) 137, 140, 149, 629, 993, 1220
NdeII GATC 6 cut(s) 112, 187, 296, 795, 1143, 1203
NlaIII CATG 2 cut(s) 623, 1000
NlaIV GGNNCC 5 cut(s) 298, 764, 875, 1134, 1145
NmuCI GTSAC 1 cut(s) 830
NspI RCATGY 1 cut(s) 1000
PaeI GCATGC 1 cut(s) 1000
PaeR7I CTCGAG 1 cut(s) 1199
PaqCI CACCTGC 1 cut(s) 538
PcsI WCGNNNNNNNCGW 1 cut(s) 1212
PfeI GAWTC 5 cut(s) 87, 582, 694, 818, 880
PkrI GCNGC 5 cut(s) 133, 142, 572, 738, 1222
PleI GAGTC 1 cut(s) 973
PpsI GAGTC 1 cut(s) 973
PpuMI RGGWCCY 1 cut(s) 1133
Psp1406I AACGTT 1 cut(s) 612
Psp5II RGGWCCY 1 cut(s) 1133
Psp6I CCWGG 1 cut(s) 225
PspEI GGTNACC 1 cut(s) 1193
PspGI CCWGG 1 cut(s) 225
PspN4I GGNNCC 5 cut(s) 298, 764, 875, 1134, 1145
PspOMI GGGCCC 1 cut(s) 873
PspPI GGNCC 5 cut(s) 606, 763, 873, 874, 1133
PspPPI RGGWCCY 1 cut(s) 1133
PsuI RGATCY 3 cut(s) 112, 296, 1143
PvuII CAGCTG 1 cut(s) 886
RsaI GTAC 4 cut(s) 649, 714, 915, 923
RsaNI GTAC 4 cut(s) 648, 713, 914, 922
RseI CAYNNNNRTG 1 cut(s) 516
SaqAI TTAA 5 cut(s) 44, 375, 435, 615, 1225
SatI GCNGC 5 cut(s) 132, 141, 571, 737, 1221
Sau3AI GATC 6 cut(s) 112, 187, 296, 795, 1143, 1203
Sau96I GGNCC 5 cut(s) 606, 763, 873, 874, 1133
ScaI AGTACT 1 cut(s) 915
SchI GAGTC 1 cut(s) 974
ScrFI CCNGG 1 cut(s) 227
SduI GDGCHC 3 cut(s) 55, 848, 877
SfaNI GCATC 4 cut(s) 232, 433, 439, 1101
Sfr274I CTCGAG 1 cut(s) 1199
SinI GGWCC 1 cut(s) 1133
SlaI CTCGAG 1 cut(s) 1199
SmiMI CAYNNNNRTG 1 cut(s) 516
SmlI CTYRAG 1 cut(s) 1199
SmoI CTYRAG 1 cut(s) 1199
SphI GCATGC 1 cut(s) 1000
Sse9I AATT 6 cut(s) 155, 232, 663, 783, 972, 1179
SsiI CCGC 1 cut(s) 571
SspMI CTAG 2 cut(s) 80, 332
StyD4I CCNGG 1 cut(s) 225
StyI CCWWGG 3 cut(s) 108, 1072, 1147
TaaI ACNGT 2 cut(s) 793, 1053
TaiI ACGT 2 cut(s) 594, 615
TaqI TCGA 7 cut(s) 5, 439, 798, 968, 1113, 1185, 1200
TaqII GACCGA 1 cut(s) 80
TasI AATT 6 cut(s) 155, 232, 663, 783, 972, 1179
TatI WGTACW 3 cut(s) 647, 913, 921
TauI GCSGC 1 cut(s) 573
TfiI GAWTC 5 cut(s) 87, 582, 694, 818, 880
Tru1I TTAA 5 cut(s) 44, 375, 435, 615, 1225
Tru9I TTAA 5 cut(s) 44, 375, 435, 615, 1225
TscAI CASTG 5 cut(s) 60, 608, 798, 841, 1102
TseFI GTSAC 1 cut(s) 830
TseI GCWGC 4 cut(s) 131, 140, 736, 1220
Tsp45I GTSAC 1 cut(s) 830
TspDTI ATGAA 1 cut(s) 225
TspGWI ACGGA 1 cut(s) 303
TspRI CASTG 5 cut(s) 60, 608, 798, 841, 1102
VneI GTGCAC 2 cut(s) 51, 844
VpaK11BI GGWCC 1 cut(s) 1133
XapI RAATTY 1 cut(s) 155
XceI RCATGY 1 cut(s) 1000
XcmI CCANNNNNNNNNTGG 1 cut(s) 1079
XhoI CTCGAG 1 cut(s) 1199
XmiI GTMKAC 1 cut(s) 383
XspI CTAG 2 cut(s) 80, 332
ZrmI AGTACT 1 cut(s) 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.