Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0002058

Physical Location & Seq

Genomic Coordinates Forward (+)

6912649 .. 6916368

Gene Structure 3720 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G007510.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1527 bp

ATGTGGAGTAAATATCTCTTGGTGGCTTTGTGCTTATGGACTTTAATCTGTGCACTGCTTCCTGCTTCCTCTAATGGCCTAGTGAGAATCGGTCTGAAGAAGCGAACCTTGGATCTTCAGACCATCAAAGCTGCCAGAGCAGCAAGGGTGGCAAAATTTAGAGGGACTACTATAAGGGGGGTGATTGATCATAACTATTTGGGTGATGATGAAGATATAGTACCCCTGGAGAATTATATGGATGCTCAATACTATGGTGAGATTGGTATTGGCTCACCACCTCAGAACTTTACTGTCATATTCGACACAGGAAGTTCCAATCTTTGGGTGCCATCAGCAAAGTGTTACTTGTCTATTGCGTGCTATTTCCATACTCGATACAAGTCAAGCAAGTCGAGTACCTATATTGCAAATGGAAAGTCTGCTCAAATAACATATGGAAGTGGATCCATTTCTGGTTTTTTCAGTCAAGATAATGTTCTAGTCGGCGATTTAGTTGTCAAAAATCAGGAATTCATCGAGGCCACGCGAGAAGGCAGTCTCACATTTGTCTTGGCAAAGTTTGATGGACTTCTTGGGCTTGGATTTCAAGAAATCTCTGTTGGAAATGCTACACCAGTGTGGTCTACAATGGCAGAGCAAGGTCTTTTAGATGATGAGGTCTTCTCATTCTGGCTTAATCGAGATGCAGATGCAGAAGTTGGAGGTGAACTTGTTTTTGGTGGTGTTGACAAAGCGCACTATAAGGGAAAGCATACCTATGTTCCAGTTACTAAAAAGGGCTACTGGCAGTTCAATTTGGGAGATCTCTTAATCGGAAACCAGTCAACAGGTGTCTGTGAGGGGGGTTGTGCTGCTGTCGTGGATTCAGGAACGTCCTTACTCACTGGCCCAACATCTGTTGTTACTGAAGTTAACCATGCTATTGGAGCTGAAGGAGTAGTGAGTACAGAATGTAAGCAAATTGTTTCTCAGTATGGAGACTTGATATGGAATCTTCTAATATCAGGGGTACAACCTGACCAAGTATGTAAGCAGCTTGGTTTATGTGTTTTGAATGGGGGCCAGTATGTGAGTGCTGGAATTGAAACAGTGATCGAAAGAGAAGATAGGGAGGAATCATCTGTTGGTGACACTGCTTTGTGCACAGCTTGTGAGATGGCTGTTGTTTGGGCCCAGAATCAGCTTAGACAAAAAGGAACAAAAGAAAGAGTACTTCAGTACATTGATGAGCTGTGTGAGAGCCTGCCAAGTCCAATGGGAGAGTCGATAATTGACTGCAATAGCATTTCAAGCATGCCGAACATTACCTTCACCATCGGAGATAAGCCTTTTGTTCTCACTCCACAACAGTATGTTCTGAAAACTGGCCAAGGCACTACCGGTATCTGCATCAGTGGGTTTACAGCTTTCGATATATCCCCTCCAACGGGCCCTCTCTGGATCCTTGGAGATGTATTTATGGGGGCATATCATACAATTTTCGACTTCGGTAACCTCGAGATCGGATTTGCCGAAGCTGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0044238 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asp SapB_1 SapB_2

Protein Analysis

508

Amino Acids

54.93

Weight (kDa)

4.76

Isoelectric Point (pI)

34.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	83 - 507	1.4e-115	Eukaryotic aspartyl protease
TAXi_N	PF14543	84 - 242	1.6e-10	Xylanase inhibitor N-terminal
SapB_2	PF03489	317 - 350	9.2e-15	Saposin-like type B, region 2
SapB_1	PF05184	381 - 417	7.5e-11	Saposin-like type B, region 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002058)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G22050
fragaria_vesca	FvH4_4g21720 FvH4_4g21720 FvH4_4g21720 FvH4_6g08500 FvH4_6g08500 FvH4_6g08500 FvH4_6g08500
malus_domestica	MD04G1186500.v1.1 MD04G1186600.v1.1 MD13G1138100.v1.1 MD16G1133900.v1.1
prunus_persica	Prupe.1G103000_v2.0.a1 Prupe.1G103000_v2.0.a1 Prupe.1G103000_v2.0.a1 Prupe.1G103000_v2.0.a1 Prupe.6G304600_v2.0.a1 Prupe.6G304600_v2.0.a1 Prupe.6G304600_v2.0.a1
pyrus_communis	pycom04g16480 pycom13g12020 pycom16g11820
rosa_chinensis	RchiOBHm_Chr3g0458541 RchiOBHm_Chr4g0427921
rosa_laevigata	RLG00000007200 RLG00000025149
rosa_roxburghii	Rroxscaffold_2G00125080 Rroxscaffold_5G00369590 Rroxscaffold_6G00420990
rosa_rugosa	Rorug03G0029500 Rorug03G0029500 Rorug03G0029500 Rorug03G0029600 Rorug04G0220600
rosa_samantha	Rh3AG087900 Rh3BG091000 Rh3CG091100 Rh3DG091900 Rh4AG277600 Rh4BG283400 Rh4CG298900 Rh4DG280600
rosa_wichuraiana	Rw3G007510 Rw4G024120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	328
AccB7I	CCANNNNNTGG	1 cut(s)	324
AccI	GTMKAC	1 cut(s)	626
AccII	CGCG	1 cut(s)	529
AclWI	GGATC	5 cut(s)	120, 441, 454, 1438, 1451
AcoI	YGGCCR	1 cut(s)	1369
AcsI	RAATTY	2 cut(s)	155, 512
AcuI	CTGAAG	5 cut(s)	101, 116, 930, 954, 1202
AfaI	GTAC	6 cut(s)	222, 400, 949, 1014, 1215, 1223
AfiI	CCNNNNNNNGG	3 cut(s)	324, 1429, 1430
AgeI	ACCGGT	1 cut(s)	1382
AgsI	TTSAA	5 cut(s)	590, 796, 1057, 1088, 1293
AjnI	CCWGG	1 cut(s)	225
AjuI	GAANNNNNNNTTGG	8 cut(s)	92, 124, 585, 617, 702, 734, 1110, 1142
AleI	CACNNNNGTG	1 cut(s)	619
AluBI	AGCT	8 cut(s)	131, 932, 1039, 1151, 1186, 1234, 1409, 1520
AluI	AGCT	8 cut(s)	131, 932, 1039, 1151, 1186, 1234, 1409, 1520
Alw21I	GWGCWC	2 cut(s)	55, 1148
Alw26I	GTCTC	2 cut(s)	545, 975
Alw44I	GTGCAC	2 cut(s)	51, 1144
AlwI	GGATC	5 cut(s)	120, 441, 454, 1438, 1451
Ama87I	CYCGRG	1 cut(s)	1499
AoxI	GGCC	7 cut(s)	76, 522, 889, 1063, 1173, 1369, 1432
ApaI	GGGCCC	2 cut(s)	1177, 1436
ApaLI	GTGCAC	2 cut(s)	51, 1144
ApeKI	GCWGC	5 cut(s)	131, 140, 854, 1036, 1520
ApoI	RAATTY	2 cut(s)	155, 512
ArsI	GACNNNNNNTTYG	2 cut(s)	404, 436
AsiGI	ACCGGT	1 cut(s)	1382
AspLEI	GCGC	1 cut(s)	739
AspS9I	GGNCC	6 cut(s)	890, 1063, 1173, 1174, 1432, 1433
AsuHPI	GGTGA	7 cut(s)	193, 215, 267, 269, 719, 1142, 1306
AvaI	CYCGRG	1 cut(s)	1499
BaeGI	GKGCMC	4 cut(s)	55, 1148, 1177, 1436
BalI	TGGCCA	1 cut(s)	1371
BamHI	GGATCC	2 cut(s)	446, 1443
BanI	GGYRCC	1 cut(s)	328
BanII	GRGCYC	2 cut(s)	1177, 1436
BarI	GAAGNNNNNNTAC	2 cut(s)	204, 236
BbsI	GAAGAC	1 cut(s)	655
Bbv12I	GWGCWC	2 cut(s)	55, 1148
BbvI	GCAGC	5 cut(s)	118, 152, 841, 1048, 1507
BccI	CCATC	5 cut(s)	131, 340, 560, 1153, 1325
BciT130I	CCWGG	1 cut(s)	227
BclI	TGATCA	1 cut(s)	187
BcoDI	GTCTC	2 cut(s)	545, 975
BfaI	CTAG	2 cut(s)	80, 482
BglII	AGATCT	1 cut(s)	805
BisI	GCNGC	5 cut(s)	132, 141, 855, 1037, 1521
BlsI	GCNGC	5 cut(s)	133, 142, 856, 1038, 1522
BmcAI	AGTACT	1 cut(s)	1215
Bme1390I	CCNGG	1 cut(s)	227
BmeT110I	CYCGRG	1 cut(s)	1499
BmgT120I	GGNCC	6 cut(s)	890, 1063, 1173, 1174, 1432, 1433
BmiI	GGNNCC	6 cut(s)	330, 448, 1064, 1175, 1434, 1445
BmrFI	CCNGG	1 cut(s)	227
BmsI	GCATC	4 cut(s)	232, 676, 682, 1401
BpiI	GAAGAC	1 cut(s)	655
BplI	GAGNNNNNCTC	2 cut(s)	650, 682
BpmI	CTGGAG	1 cut(s)	248
BsaJI	CCNNGG	4 cut(s)	108, 225, 1372, 1447
BsaWI	WCCGGW	1 cut(s)	1382
Bsc4I	CCNNNNNNNGG	3 cut(s)	324, 1429, 1430
Bse118I	RCCGGY	1 cut(s)	1382
Bse1I	ACTGG	7 cut(s)	617, 767, 791, 823, 892, 1066, 1372
BseBI	CCWGG	1 cut(s)	227
BseDI	CCNNGG	4 cut(s)	108, 225, 1372, 1447
BseGI	GGATG	1 cut(s)	247
BseLI	CCNNNNNNNGG	3 cut(s)	324, 1429, 1430
BseMII	CTCAG	2 cut(s)	296, 986
BseNI	ACTGG	7 cut(s)	617, 767, 791, 823, 892, 1066, 1372
BseSI	GKGCMC	4 cut(s)	55, 1148, 1177, 1436
BseXI	GCAGC	5 cut(s)	118, 152, 841, 1048, 1507
Bsh1236I	CGCG	1 cut(s)	529
BshFI	GGCC	7 cut(s)	78, 524, 891, 1065, 1175, 1371, 1434
BshNI	GGYRCC	1 cut(s)	328
BshTI	ACCGGT	1 cut(s)	1382
BsiHKAI	GWGCWC	2 cut(s)	55, 1148
BsiHKCI	CYCGRG	1 cut(s)	1499
BsiSI	CCGG	1 cut(s)	1383
BslFI	GGGAC	1 cut(s)	178
BslI	CCNNNNNNNGG	3 cut(s)	324, 1429, 1430
BsmAI	GTCTC	2 cut(s)	545, 975
BsmFI	GGGAC	1 cut(s)	178
BsnI	GGCC	7 cut(s)	78, 524, 891, 1065, 1175, 1371, 1434
BsoBI	CYCGRG	1 cut(s)	1499
Bsp120I	GGGCCC	2 cut(s)	1173, 1432
Bsp1286I	GDGCHC	4 cut(s)	55, 1148, 1177, 1436
Bsp143I	GATC	7 cut(s)	112, 187, 446, 805, 1095, 1443, 1503
BspANI	GGCC	7 cut(s)	78, 524, 891, 1065, 1175, 1371, 1434
BspCNI	CTCAG	2 cut(s)	295, 985
BspFNI	CGCG	1 cut(s)	529
BspLI	GGNNCC	6 cut(s)	330, 448, 1064, 1175, 1434, 1445
BspPI	GGATC	5 cut(s)	120, 441, 454, 1438, 1451
BspT107I	GGYRCC	1 cut(s)	328
BsrFI	RCCGGY	1 cut(s)	1382
BsrI	ACTGG	7 cut(s)	617, 767, 791, 823, 892, 1066, 1372
BssAI	RCCGGY	1 cut(s)	1382
BssECI	CCNNGG	4 cut(s)	108, 225, 1372, 1447
BssMI	GATC	7 cut(s)	112, 187, 446, 805, 1095, 1443, 1503
BssT1I	CCWWGG	3 cut(s)	108, 1372, 1447
Bst2UI	CCWGG	1 cut(s)	227
Bst4CI	ACNGT	3 cut(s)	295, 1093, 1353
BstC8I	GCNNGC	3 cut(s)	361, 1247, 1298
BstDEI	CTNAG	4 cut(s)	282, 972, 1187, 1524
BstEII	GGTNACC	1 cut(s)	1493
BstF5I	GGATG	1 cut(s)	247
BstFNI	CGCG	1 cut(s)	529
BstHHI	GCGC	1 cut(s)	739
BstKTI	GATC	7 cut(s)	115, 190, 449, 808, 1098, 1446, 1506
BstMAI	GTCTC	2 cut(s)	545, 975
BstMBI	GATC	7 cut(s)	112, 187, 446, 805, 1095, 1443, 1503
BstMWI	GCNNNNNNNGC	6 cut(s)	137, 140, 149, 929, 1293, 1520
BstNI	CCWGG	1 cut(s)	227
BstNSI	RCATGY	1 cut(s)	1300
BstPI	GGTNACC	1 cut(s)	1493
BstSCI	CCNGG	1 cut(s)	225
BstSLI	GKGCMC	4 cut(s)	55, 1148, 1177, 1436
BstUI	CGCG	1 cut(s)	529
BstV1I	GCAGC	5 cut(s)	118, 152, 841, 1048, 1507
BstV2I	GAAGAC	1 cut(s)	655
BstX2I	RGATCY	4 cut(s)	112, 446, 805, 1443
BstXI	CCANNNNNNTGG	1 cut(s)	926
BstYI	RGATCY	4 cut(s)	112, 446, 805, 1443
BsuRI	GGCC	7 cut(s)	78, 524, 891, 1065, 1175, 1371, 1434
BtsCI	GGATG	1 cut(s)	247
BtsI	GCAGTG	2 cut(s)	53, 1134
BtsIMutI	CAGTG	6 cut(s)	53, 624, 885, 1098, 1134, 1402
Cac8I	GCNNGC	3 cut(s)	361, 1247, 1298
CfoI	GCGC	1 cut(s)	739
Cfr10I	RCCGGY	1 cut(s)	1382
Cfr13I	GGNCC	6 cut(s)	890, 1063, 1173, 1174, 1432, 1433
Csp6I	GTAC	6 cut(s)	221, 399, 948, 1013, 1214, 1222
CspAI	ACCGGT	1 cut(s)	1382
CviAII	CATG	2 cut(s)	920, 1297
CviQI	GTAC	6 cut(s)	221, 399, 948, 1013, 1214, 1222
DdeI	CTNAG	4 cut(s)	282, 972, 1187, 1524
DpnI	GATC	7 cut(s)	114, 189, 448, 807, 1097, 1445, 1505
DpnII	GATC	7 cut(s)	112, 187, 446, 805, 1095, 1443, 1503
EaeI	YGGCCR	1 cut(s)	1369
Eco130I	CCWWGG	3 cut(s)	108, 1372, 1447
Eco24I	GRGCYC	2 cut(s)	1177, 1436
Eco57I	CTGAAG	5 cut(s)	101, 116, 930, 954, 1202
Eco88I	CYCGRG	1 cut(s)	1499
Eco91I	GGTNACC	1 cut(s)	1493
EcoO109I	RGGNCCY	1 cut(s)	1433
EcoO65I	GGTNACC	1 cut(s)	1493
EcoRI	GAATTC	1 cut(s)	512
EcoRII	CCWGG	1 cut(s)	225
EcoT14I	CCWWGG	3 cut(s)	108, 1372, 1447
EcoT38I	GRGCYC	2 cut(s)	1177, 1436
ErhI	CCWWGG	3 cut(s)	108, 1372, 1447
FaeI	CATG	2 cut(s)	923, 1300
FalI	AAGNNNNNCTT	4 cut(s)	92, 124, 332, 364
FaqI	GGGAC	1 cut(s)	178
FatI	CATG	2 cut(s)	919, 1296
FauNDI	CATATG	1 cut(s)	436
FbaI	TGATCA	1 cut(s)	187
FblI	GTMKAC	1 cut(s)	626
Fnu4HI	GCNGC	5 cut(s)	132, 141, 855, 1037, 1521
FokI	GGATG	1 cut(s)	254
FriOI	GRGCYC	2 cut(s)	1177, 1436
Fsp4HI	GCNGC	5 cut(s)	132, 141, 855, 1037, 1521
FspBI	CTAG	2 cut(s)	80, 482
GlaI	GCGC	1 cut(s)	738
GluI	GCNGC	5 cut(s)	132, 141, 855, 1037, 1521
GsuI	CTGGAG	1 cut(s)	248
HaeIII	GGCC	7 cut(s)	78, 524, 891, 1065, 1175, 1371, 1434
HapII	CCGG	1 cut(s)	1383
HhaI	GCGC	1 cut(s)	739
Hin1II	CATG	2 cut(s)	923, 1300
Hin6I	GCGC	1 cut(s)	737
HinP1I	GCGC	1 cut(s)	737
HincII	GTYRAC	3 cut(s)	730, 828, 916
HindII	GTYRAC	3 cut(s)	730, 828, 916
HinfI	GANTC	6 cut(s)	87, 866, 994, 1118, 1180, 1265
HpaI	GTTAAC	1 cut(s)	916
HpaII	CCGG	1 cut(s)	1383
HphI	GGTGA	7 cut(s)	193, 215, 267, 269, 719, 1142, 1306
Hpy166II	GTNNAC	8 cut(s)	53, 627, 710, 730, 828, 916, 1146, 1404
Hpy188I	TCNGA	7 cut(s)	96, 120, 285, 818, 1322, 1362, 1508
Hpy188III	TCNNGA	7 cut(s)	470, 509, 590, 683, 870, 1441, 1501
Hpy8I	GTNNAC	8 cut(s)	53, 627, 710, 730, 828, 916, 1146, 1404
HpyAV	CCTTC	3 cut(s)	527, 929, 1321
HpyCH4III	ACNGT	3 cut(s)	295, 1093, 1353
HpyCH4IV	ACGT	1 cut(s)	875
HpyCH4V	TGCA	7 cut(s)	53, 410, 689, 695, 1146, 1281, 1392
HpyF10VI	GCNNNNNNNGC	6 cut(s)	137, 140, 149, 929, 1293, 1520
HpyF3I	CTNAG	4 cut(s)	282, 972, 1187, 1524
HpySE526I	ACGT	1 cut(s)	875
Hsp92II	CATG	2 cut(s)	923, 1300
HspAI	GCGC	1 cut(s)	737
Ksp22I	TGATCA	1 cut(s)	187
KspAI	GTTAAC	1 cut(s)	916
Kzo9I	GATC	7 cut(s)	112, 187, 446, 805, 1095, 1443, 1503
LmnI	GCTCC	1 cut(s)	929
Lsp1109I	GCAGC	5 cut(s)	118, 152, 841, 1048, 1507
LweI	GCATC	4 cut(s)	232, 676, 682, 1401
MaeI	CTAG	2 cut(s)	80, 482
MaeII	ACGT	1 cut(s)	875
MaeIII	GTNAC	5 cut(s)	344, 769, 904, 1130, 1493
MalI	GATC	7 cut(s)	114, 189, 448, 807, 1097, 1445, 1505
MboI	GATC	7 cut(s)	112, 187, 446, 805, 1095, 1443, 1503
MboII	GAAGA	6 cut(s)	107, 109, 224, 655, 989, 1118
MflI	RGATCY	4 cut(s)	112, 446, 805, 1443
MhlI	GDGCHC	4 cut(s)	55, 1148, 1177, 1436
MlsI	TGGCCA	1 cut(s)	1371
MluCI	AATT	8 cut(s)	155, 232, 512, 796, 963, 1083, 1272, 1479
MluNI	TGGCCA	1 cut(s)	1371
MlyI	GAGTC	1 cut(s)	1274
MmeI	TCCRAC	3 cut(s)	583, 682, 1451
Mox20I	TGGCCA	1 cut(s)	1371
MscI	TGGCCA	1 cut(s)	1371
MseI	TTAA	4 cut(s)	44, 678, 812, 915
MslI	CAYNNNNRTG	2 cut(s)	619, 759
Msp20I	TGGCCA	1 cut(s)	1371
MspI	CCGG	1 cut(s)	1383
MspR9I	CCNGG	1 cut(s)	227
MvaI	CCWGG	1 cut(s)	227
MvnI	CGCG	1 cut(s)	529
MwoI	GCNNNNNNNGC	6 cut(s)	137, 140, 149, 929, 1293, 1520
NdeI	CATATG	1 cut(s)	436
NdeII	GATC	7 cut(s)	112, 187, 446, 805, 1095, 1443, 1503
NlaIII	CATG	2 cut(s)	923, 1300
NlaIV	GGNNCC	6 cut(s)	330, 448, 1064, 1175, 1434, 1445
NmuCI	GTSAC	1 cut(s)	1130
NspI	RCATGY	1 cut(s)	1300
OliI	CACNNNNGTG	1 cut(s)	619
PaeI	GCATGC	1 cut(s)	1300
PaeR7I	CTCGAG	1 cut(s)	1499
PcsI	WCGNNNNNNNCGW	2 cut(s)	1497, 1512
PfeI	GAWTC	5 cut(s)	87, 866, 994, 1118, 1180
PflMI	CCANNNNNTGG	1 cut(s)	324
PinAI	ACCGGT	1 cut(s)	1382
PkrI	GCNGC	5 cut(s)	133, 142, 856, 1038, 1522
PleI	GAGTC	1 cut(s)	1273
PpsI	GAGTC	1 cut(s)	1273
Psp6I	CCWGG	1 cut(s)	225
PspEI	GGTNACC	1 cut(s)	1493
PspGI	CCWGG	1 cut(s)	225
PspN4I	GGNNCC	6 cut(s)	330, 448, 1064, 1175, 1434, 1445
PspOMI	GGGCCC	2 cut(s)	1173, 1432
PspPI	GGNCC	6 cut(s)	890, 1063, 1173, 1174, 1432, 1433
PsuI	RGATCY	4 cut(s)	112, 446, 805, 1443
RsaI	GTAC	6 cut(s)	222, 400, 949, 1014, 1215, 1223
RsaNI	GTAC	6 cut(s)	221, 399, 948, 1013, 1214, 1222
RseI	CAYNNNNRTG	2 cut(s)	619, 759
SaqAI	TTAA	4 cut(s)	44, 678, 812, 915
SatI	GCNGC	5 cut(s)	132, 141, 855, 1037, 1521
Sau3AI	GATC	7 cut(s)	112, 187, 446, 805, 1095, 1443, 1503
Sau96I	GGNCC	6 cut(s)	890, 1063, 1173, 1174, 1432, 1433
ScaI	AGTACT	1 cut(s)	1215
SchI	GAGTC	1 cut(s)	1274
ScrFI	CCNGG	1 cut(s)	227
SduI	GDGCHC	4 cut(s)	55, 1148, 1177, 1436
SfaNI	GCATC	4 cut(s)	232, 676, 682, 1401
Sfr274I	CTCGAG	1 cut(s)	1499
SlaI	CTCGAG	1 cut(s)	1499
SmiMI	CAYNNNNRTG	2 cut(s)	619, 759
SmlI	CTYRAG	1 cut(s)	1499
SmoI	CTYRAG	1 cut(s)	1499
SphI	GCATGC	1 cut(s)	1300
Sse9I	AATT	8 cut(s)	155, 232, 512, 796, 963, 1083, 1272, 1479
SspMI	CTAG	2 cut(s)	80, 482
StyD4I	CCNGG	1 cut(s)	225
StyI	CCWWGG	3 cut(s)	108, 1372, 1447
TaaI	ACNGT	3 cut(s)	295, 1093, 1353
TaiI	ACGT	1 cut(s)	878
TaqII	GACCGA	1 cut(s)	80
TasI	AATT	8 cut(s)	155, 232, 512, 796, 963, 1083, 1272, 1479
TatI	WGTACW	3 cut(s)	947, 1213, 1221
TfiI	GAWTC	5 cut(s)	87, 866, 994, 1118, 1180
Tru1I	TTAA	4 cut(s)	44, 678, 812, 915
Tru9I	TTAA	4 cut(s)	44, 678, 812, 915
TscAI	CASTG	6 cut(s)	60, 624, 892, 1098, 1141, 1402
TseFI	GTSAC	1 cut(s)	1130
TseI	GCWGC	5 cut(s)	131, 140, 854, 1036, 1520
Tsp45I	GTSAC	1 cut(s)	1130
TspDTI	ATGAA	2 cut(s)	225, 505
TspRI	CASTG	6 cut(s)	60, 624, 892, 1098, 1141, 1402
Van91I	CCANNNNNTGG	1 cut(s)	324
VneI	GTGCAC	2 cut(s)	51, 1144
XapI	RAATTY	2 cut(s)	155, 512
XceI	RCATGY	1 cut(s)	1300
XhoI	CTCGAG	1 cut(s)	1499
XmiI	GTMKAC	1 cut(s)	626
XspI	CTAG	2 cut(s)	80, 482
ZrmI	AGTACT	1 cut(s)	1215

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.