FvH4_4g22730

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0005010
Physical Location & Seq
Reverse (-)
25451240 .. 25452989
1750 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g22730.t1

Sequence Viewer

Length: 1101 bp
ATGGACGACAGCTTCAGACTCCGCGCCGACAAGATCTTCGGATCCCTCTCCGCCTCGCTCTCGTCTTCTCTCCAACCATCGCAGTCCGCTCCCTGGTCCGTCGCCGCCGCCGAGGTCGAGCGCCGCCAATGGAGGCGCCCCTCCGACGCCCCCGACCGAGACGAAACCCCCTGTGCGTCGTCGTTTCAGGGCGAATTCGGAATCGGATCTTCCATCGGCATGGATCCTACTCTCGATTTCGAGGATGAGGAAGATGAATATGACAAGGTGGCATCGGGTGCCGAGGCTGCTGATGATGTTCGTTTGTTTATGGACGACGTTACTCGTCACGGCTCTTATTTGAATTCGTACAATGTGTTGGTTGGTAAGGATTCGCGTGCGAATCACCCAGCTGCAAGGCACCGGCTGAGAGAAGATGATGCTCAAGTTCAGAACCCTGGTTCCACACCCGTGGATTTTACTGCACCCCGGTGTGCGAAATCACCTGAAGATGGTGGTCAGCCGAAGCCTATACTGAAAAGGAAGGACAGTTCCTCGGATACCAAGTCTCGGAAACGTGTTAGGTTTGACCCTGCTTTTGTAGATCAGAGTGATGGTGAAGAATCATCGGGTAGAGTTCAGAAGTTGGGTTCTGGATTAAATAAGACTGCTTCTCAGGTTCCGGATTATTTGGTGAATCCTTCGAGGTATACGTGTTATAGTTTTGATTCAAGAAGTGAAGTCGATGAAGGTGGCAACACCAAGGCGTTTATGGACTACATTACCAAGACGGATAAGGACTGCATTACTCAGGTTGAAAGCTCAGGGTCGGAGATGGAGGATGCTTCACCTCAACTTCCCAAGTCTGTGACTTTTGTTCCAAAGAAGAAGACTGTTGATGGTAGCACTAGAACATTACAGGATGCGGAAGATGACAATAAGCAATCCTTGAATATAAAAGGCTTTGCTGTTGGTATTGCGGCTATGGTAGAAAGTGAAATCAGTGCAGCAGAGGAGGAAGACAAGTCGGAGCAAAATGCAGCTGTTTTGAGTGATAGTTCATCAAAAAGTGGTCGGAAGTTTCGAACCAAGTCAATGTCAGATGATGAGTCTGATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TSSC4

Protein Analysis

367

Amino Acids

39.99

Weight (kDa)

4.73

Isoelectric Point (pI)

56.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TSSC4 PF15264 188 - 273 4.5e-07 Tumour suppressing sub-chromosomal transferable candidate 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 135, 278, 399
AccBSI CCGCTC 1 cut(s) 89
AccI GTMKAC 1 cut(s) 689
AccII CGCG 2 cut(s) 24, 376
AccIII TCCGGA 1 cut(s) 661
AciI CCGC 8 cut(s) 22, 51, 87, 105, 108, 124, 905, 959
AclWI GGATC 5 cut(s) 36, 49, 214, 218, 231
AcsI RAATTY 2 cut(s) 194, 343
AcuI CTGAAG 1 cut(s) 507
AcyI GRCGYC 2 cut(s) 136, 147
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 3 cut(s) 93, 491, 549
AflIII ACRYGT 2 cut(s) 556, 692
AgsI TTSAA 4 cut(s) 343, 711, 797, 931
AjnI CCWGG 2 cut(s) 92, 436
AleI CACNNNNGTG 2 cut(s) 449, 469
AloI GAACNNNNNNTCC 4 cut(s) 425, 457, 514, 546
AluBI AGCT 4 cut(s) 12, 392, 801, 1022
AluI AGCT 4 cut(s) 12, 392, 801, 1022
Alw26I GTCTC 2 cut(s) 153, 552
AlwI GGATC 5 cut(s) 36, 49, 214, 218, 231
Aor13HI TCCGGA 1 cut(s) 661
ApeKI GCWGC 4 cut(s) 287, 392, 986, 1019
ApoI RAATTY 2 cut(s) 194, 343
ArsI GACNNNNNNTTYG 4 cut(s) 20, 52, 560, 592
AspLEI GCGC 3 cut(s) 26, 123, 138
AspS9I GGNCC 1 cut(s) 96
AsuC2I CCSGG 1 cut(s) 469
AsuHPI GGTGA 5 cut(s) 377, 474, 608, 685, 819
AsuII TTCGAA 1 cut(s) 1063
AvaII GGWCC 1 cut(s) 96
BamHI GGATCC 2 cut(s) 41, 223
BanI GGYRCC 3 cut(s) 135, 278, 399
BbsI GAAGAC 3 cut(s) 57, 875, 1005
BbvI GCAGC 4 cut(s) 274, 379, 998, 1031
BccI CCATC 6 cut(s) 85, 221, 485, 587, 808, 872
BceAI ACGGC 1 cut(s) 346
BciT130I CCWGG 2 cut(s) 94, 438
BciVI GTATCC 1 cut(s) 532
BcnI CCSGG 1 cut(s) 469
BcoDI GTCTC 2 cut(s) 153, 552
BfaI CTAG 1 cut(s) 888
BfoI RGCGCY 2 cut(s) 124, 139
BfuI GTATCC 1 cut(s) 532
BglII AGATCT 1 cut(s) 33
BisI GCNGC 8 cut(s) 105, 108, 124, 288, 393, 960, 987, 1020
BlsI GCNGC 8 cut(s) 106, 109, 125, 289, 394, 961, 988, 1021
Bme1390I CCNGG 3 cut(s) 94, 438, 469
Bme18I GGWCC 1 cut(s) 96
BmgT120I GGNCC 1 cut(s) 96
BmiI GGNNCC 7 cut(s) 43, 137, 225, 280, 401, 442, 660
BmrFI CCNGG 3 cut(s) 94, 438, 469
BmsI GCATC 4 cut(s) 281, 409, 811, 892
BpiI GAAGAC 3 cut(s) 57, 875, 1005
Bpu10I CCTNAGC 1 cut(s) 802
Bpu14I TTCGAA 1 cut(s) 1063
BpuEI CTTGAG 1 cut(s) 408
BpuMI CCSGG 1 cut(s) 469
BsaAI YACGTR 1 cut(s) 693
BsaHI GRCGYC 2 cut(s) 136, 147
BsaJI CCNNGG 8 cut(s) 92, 111, 282, 436, 450, 467, 534, 741
BsaWI WCCGGW 1 cut(s) 661
Bsc4I CCNNNNNNNGG 3 cut(s) 93, 491, 549
Bse118I RCCGGY 1 cut(s) 402
BseAI TCCGGA 1 cut(s) 661
BseBI CCWGG 2 cut(s) 94, 438
BseDI CCNNGG 8 cut(s) 92, 111, 282, 436, 450, 467, 534, 741
BseGI GGATG 3 cut(s) 250, 826, 907
BseLI CCNNNNNNNGG 3 cut(s) 93, 491, 549
BseMII CTCAG 4 cut(s) 398, 668, 803, 816
BseRI GAGGAG 1 cut(s) 1007
BseXI GCAGC 4 cut(s) 274, 379, 998, 1031
BseYI CCCAGC 1 cut(s) 388
BsgI GTGCAG 2 cut(s) 447, 1005
Bsh1236I CGCG 2 cut(s) 24, 376
Bsh1285I CGRYCG 1 cut(s) 157
BshNI GGYRCC 3 cut(s) 135, 278, 399
BsiEI CGRYCG 1 cut(s) 157
BsiSI CCGG 3 cut(s) 403, 469, 662
BslI CCNNNNNNNGG 3 cut(s) 93, 491, 549
BsmAI GTCTC 2 cut(s) 153, 552
BsmBI CGTCTC 1 cut(s) 153
Bsp119I TTCGAA 1 cut(s) 1063
Bsp13I TCCGGA 1 cut(s) 661
Bsp143I GATC 5 cut(s) 33, 41, 206, 223, 583
BspACI CCGC 8 cut(s) 22, 51, 87, 105, 108, 124, 905, 959
BspCNI CTCAG 4 cut(s) 399, 667, 802, 815
BspEI TCCGGA 1 cut(s) 661
BspFNI CGCG 2 cut(s) 24, 376
BspLI GGNNCC 7 cut(s) 43, 137, 225, 280, 401, 442, 660
BspPI GGATC 5 cut(s) 36, 49, 214, 218, 231
BspT104I TTCGAA 1 cut(s) 1063
BspT107I GGYRCC 3 cut(s) 135, 278, 399
BsrBI CCGCTC 1 cut(s) 89
BsrFI RCCGGY 1 cut(s) 402
BssAI RCCGGY 1 cut(s) 402
BssECI CCNNGG 8 cut(s) 92, 111, 282, 436, 450, 467, 534, 741
BssMI GATC 5 cut(s) 33, 41, 206, 223, 583
BssNAI GTATAC 1 cut(s) 690
BssNI GRCGYC 2 cut(s) 136, 147
BssT1I CCWWGG 1 cut(s) 741
Bst1107I GTATAC 1 cut(s) 690
Bst2UI CCWGG 2 cut(s) 94, 438
Bst4CI ACNGT 2 cut(s) 530, 874
BstACI GRCGYC 2 cut(s) 136, 147
BstAPI GCANNNNNTGC 1 cut(s) 278
BstBAI YACGTR 1 cut(s) 693
BstBI TTCGAA 1 cut(s) 1063
BstC8I GCNNGC 1 cut(s) 378
BstDEI CTNAG 4 cut(s) 407, 654, 789, 802
BstDSI CCRYGG 1 cut(s) 450
BstF5I GGATG 3 cut(s) 250, 826, 907
BstFNI CGCG 2 cut(s) 24, 376
BstH2I RGCGCY 2 cut(s) 124, 139
BstHHI GCGC 3 cut(s) 26, 123, 138
BstKTI GATC 5 cut(s) 36, 44, 209, 226, 586
BstMAI GTCTC 2 cut(s) 153, 552
BstMBI GATC 5 cut(s) 33, 41, 206, 223, 583
BstMCI CGRYCG 1 cut(s) 157
BstMWI GCNNNNNNNGC 2 cut(s) 278, 287
BstNI CCWGG 2 cut(s) 94, 438
BstSCI CCNGG 3 cut(s) 92, 436, 467
BstUI CGCG 2 cut(s) 24, 376
BstV1I GCAGC 4 cut(s) 274, 379, 998, 1031
BstV2I GAAGAC 3 cut(s) 57, 875, 1005
BstX2I RGATCY 4 cut(s) 33, 41, 206, 223
BstXI CCANNNNNNTGG 2 cut(s) 220, 451
BstYI RGATCY 4 cut(s) 33, 41, 206, 223
BstZ17I GTATAC 1 cut(s) 690
BsuI GTATCC 1 cut(s) 532
BtgI CCRYGG 1 cut(s) 450
BtgZI GCGATG 1 cut(s) 63
BtsCI GGATG 3 cut(s) 250, 826, 907
BtsIMutI CAGTG 1 cut(s) 988
Cac8I GCNNGC 1 cut(s) 378
CfoI GCGC 3 cut(s) 26, 123, 138
Cfr10I RCCGGY 1 cut(s) 402
Cfr13I GGNCC 1 cut(s) 96
CseI GACGC 2 cut(s) 155, 165
Csp6I GTAC 1 cut(s) 349
CviAII CATG 1 cut(s) 220
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 4 cut(s) 407, 654, 789, 802
DinI GGCGCC 1 cut(s) 137
DpnI GATC 5 cut(s) 35, 43, 208, 225, 585
DpnII GATC 5 cut(s) 33, 41, 206, 223, 583
EciI GGCGGA 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 741
Eco47I GGWCC 1 cut(s) 96
Eco57I CTGAAG 1 cut(s) 507
EcoRI GAATTC 2 cut(s) 194, 343
EcoRII CCWGG 2 cut(s) 92, 436
EcoT14I CCWWGG 1 cut(s) 741
EgeI GGCGCC 1 cut(s) 137
EheI GGCGCC 1 cut(s) 137
ErhI CCWWGG 1 cut(s) 741
Esp3I CGTCTC 1 cut(s) 153
FaeI CATG 1 cut(s) 223
FaiI YATR 9 cut(s) 221, 261, 311, 512, 690, 699, 752, 935, 965
FalI AAGNNNNNCTT 2 cut(s) 911, 943
FatI CATG 1 cut(s) 219
FblI GTMKAC 1 cut(s) 689
Fnu4HI GCNGC 8 cut(s) 105, 108, 124, 288, 393, 960, 987, 1020
FokI GGATG 3 cut(s) 257, 833, 914
Fsp4HI GCNGC 8 cut(s) 105, 108, 124, 288, 393, 960, 987, 1020
FspBI CTAG 1 cut(s) 888
GlaI GCGC 3 cut(s) 25, 122, 137
GluI GCNGC 8 cut(s) 105, 108, 124, 288, 393, 960, 987, 1020
GsaI CCCAGC 1 cut(s) 392
HaeII RGCGCY 2 cut(s) 124, 139
HapII CCGG 3 cut(s) 403, 469, 662
HgaI GACGC 2 cut(s) 155, 165
HhaI GCGC 3 cut(s) 26, 123, 138
Hin1I GRCGYC 2 cut(s) 136, 147
Hin1II CATG 1 cut(s) 223
Hin6I GCGC 3 cut(s) 24, 121, 136
HinP1I GCGC 3 cut(s) 24, 121, 136
HinfI GANTC 9 cut(s) 18, 201, 371, 382, 602, 676, 707, 1088, 1094
HpaII CCGG 3 cut(s) 403, 469, 662
HphI GGTGA 5 cut(s) 377, 474, 608, 685, 819
Hpy166II GTNNAC 1 cut(s) 690
Hpy188III TCNNGA 5 cut(s) 233, 633, 662, 711, 1098
Hpy8I GTNNAC 1 cut(s) 690
Hpy99I CGWCG 5 cut(s) 104, 149, 181, 184, 320
HpyAV CCTTC 3 cut(s) 517, 690, 722
HpyCH4III ACNGT 2 cut(s) 530, 874
HpyCH4IV ACGT 3 cut(s) 318, 556, 692
HpyCH4V TGCA 5 cut(s) 395, 464, 783, 986, 1019
HpyF10VI GCNNNNNNNGC 2 cut(s) 278, 287
HpyF3I CTNAG 4 cut(s) 407, 654, 789, 802
HpySE526I ACGT 3 cut(s) 318, 556, 692
Hsp92I GRCGYC 2 cut(s) 136, 147
Hsp92II CATG 1 cut(s) 223
HspAI GCGC 3 cut(s) 24, 121, 136
KasI GGCGCC 1 cut(s) 135
Kpn2I TCCGGA 1 cut(s) 661
Kzo9I GATC 5 cut(s) 33, 41, 206, 223, 583
LmnI GCTCC 2 cut(s) 94, 1009
Lsp1109I GCAGC 4 cut(s) 274, 379, 998, 1031
LweI GCATC 4 cut(s) 281, 409, 811, 892
MaeI CTAG 1 cut(s) 888
MaeII ACGT 3 cut(s) 318, 556, 692
MaeIII GTNAC 3 cut(s) 319, 326, 847
MalI GATC 5 cut(s) 35, 43, 208, 225, 585
MbiI CCGCTC 1 cut(s) 89
MboI GATC 5 cut(s) 33, 41, 206, 223, 583
MflI RGATCY 4 cut(s) 33, 41, 206, 223
MluCI AATT 2 cut(s) 194, 343
Mly113I GGCGCC 1 cut(s) 136
MlyI GAGTC 2 cut(s) 12, 1097
MmeI TCCRAC 5 cut(s) 97, 168, 789, 987, 1034
MroI TCCGGA 1 cut(s) 661
MseI TTAA 1 cut(s) 638
MslI CAYNNNNRTG 3 cut(s) 218, 449, 469
MspA1I CMGCKG 2 cut(s) 392, 1022
MspI CCGG 3 cut(s) 403, 469, 662
MspR9I CCNGG 3 cut(s) 94, 438, 469
MvaI CCWGG 2 cut(s) 94, 438
MvnI CGCG 2 cut(s) 24, 376
MwoI GCNNNNNNNGC 2 cut(s) 278, 287
NarI GGCGCC 1 cut(s) 136
NciI CCSGG 1 cut(s) 469
NdeII GATC 5 cut(s) 33, 41, 206, 223, 583
NlaIII CATG 1 cut(s) 223
NlaIV GGNNCC 7 cut(s) 43, 137, 225, 280, 401, 442, 660
NmeAIII GCCGAG 2 cut(s) 136, 307
NmuCI GTSAC 2 cut(s) 326, 847
NspV TTCGAA 1 cut(s) 1063
OliI CACNNNNGTG 2 cut(s) 449, 469
PcsI WCGNNNNNNNCGW 3 cut(s) 108, 689, 1060
PfeI GAWTC 7 cut(s) 201, 371, 382, 602, 676, 707, 1094
PkrI GCNGC 8 cut(s) 106, 109, 125, 289, 394, 961, 988, 1021
PleI GAGTC 2 cut(s) 12, 1096
PluTI GGCGCC 1 cut(s) 139
PpsI GAGTC 2 cut(s) 12, 1096
Ppu21I YACGTR 1 cut(s) 693
Psp6I CCWGG 2 cut(s) 92, 436
PspFI CCCAGC 1 cut(s) 388
PspGI CCWGG 2 cut(s) 92, 436
PspN4I GGNNCC 7 cut(s) 43, 137, 225, 280, 401, 442, 660
PspPI GGNCC 1 cut(s) 96
PsuI RGATCY 4 cut(s) 33, 41, 206, 223
PvuII CAGCTG 2 cut(s) 392, 1022
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
RseI CAYNNNNRTG 3 cut(s) 218, 449, 469
SaqAI TTAA 1 cut(s) 638
SatI GCNGC 8 cut(s) 105, 108, 124, 288, 393, 960, 987, 1020
Sau3AI GATC 5 cut(s) 33, 41, 206, 223, 583
Sau96I GGNCC 1 cut(s) 96
SchI GAGTC 2 cut(s) 12, 1097
ScrFI CCNGG 3 cut(s) 94, 438, 469
SfaNI GCATC 4 cut(s) 281, 409, 811, 892
SfoI GGCGCC 1 cut(s) 137
SfuI TTCGAA 1 cut(s) 1063
SinI GGWCC 1 cut(s) 96
SmiMI CAYNNNNRTG 3 cut(s) 218, 449, 469
SmlI CTYRAG 1 cut(s) 423
SmoI CTYRAG 1 cut(s) 423
Sse9I AATT 2 cut(s) 194, 343
SsiI CCGC 8 cut(s) 22, 51, 87, 105, 108, 124, 905, 959
SspDI GGCGCC 1 cut(s) 135
SspMI CTAG 1 cut(s) 888
StyD4I CCNGG 3 cut(s) 92, 436, 467
StyI CCWWGG 1 cut(s) 741
TaaI ACNGT 2 cut(s) 530, 874
TaiI ACGT 3 cut(s) 321, 559, 695
TaqI TCGA 6 cut(s) 117, 234, 240, 683, 723, 1063
TaqII GACCGA 1 cut(s) 171
TasI AATT 2 cut(s) 194, 343
TauI GCSGC 4 cut(s) 107, 110, 126, 962
TfiI GAWTC 7 cut(s) 201, 371, 382, 602, 676, 707, 1094
Tru1I TTAA 1 cut(s) 638
Tru9I TTAA 1 cut(s) 638
TscAI CASTG 1 cut(s) 988
TseFI GTSAC 2 cut(s) 326, 847
TseI GCWGC 4 cut(s) 287, 392, 986, 1019
Tsp45I GTSAC 2 cut(s) 326, 847
TspDTI ATGAA 3 cut(s) 270, 741, 1029
TspGWI ACGGA 2 cut(s) 88, 785
TspRI CASTG 1 cut(s) 988
VpaK11BI GGWCC 1 cut(s) 96
XapI RAATTY 2 cut(s) 194, 343
XcmI CCANNNNNNNNNTGG 1 cut(s) 748
XmiI GTMKAC 1 cut(s) 689
XspI CTAG 1 cut(s) 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.