Rh4CG309200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0005010
Physical Location & Seq
Reverse (-)
57073528 .. 57074924
1397 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG309200.1

Sequence Viewer

Length: 1110 bp
ATGGACGACAGCTTCAGACTCCGGGCGGACAAGGTTTTCGGATCCCTCTCTGCGTCGCTCTCCTCGTCTCTCCAACCACCGCAGTCCTCTCCCTGGTCCGTCGCCGGCGCCGAGGTCGAGCGCCGCCAATGGAGGCGCGCCACCGACGCCCCTGACCGGGACGAAACGCCCTGTGCGTCGTCGTTTCAGGGTGAATTTGGAATCGGATCTTCCATCGGCATGGACCCTACTCTGGATTTCGAGGAAGAGGAAGATGAATATGACAAAGTGGCTTCGGGTGCTGAAGCTGTTGATGATGATCGTTTGTTTATGGACGATGTTACCAACCATGGCTCTTATTTGAACTCTTACAATGTATTGGTTGGTAAGGATTCGCGTGCGAATCACCTGGCTGCCAGGCACAGGCTGAAAGAAGATGATGCTGAAGCTCAGAACCCTAGTTCTACACCAGTGGCTTCTGCTGCACTAGTTAAGCAACAGTTTGCGAAATCACCTGAAGATGGCGGTCAGCCAAAGCCTATACTGAAAAGGAAAGACAATTCCTCCGATAGCAAGTCCCGAAAACGTGTTAGGTTTGACCCTGCTTTTGTAGATCAGAGTGATGGTGAAGAATCATCAGGAAAAGTTCAAAGCTTGGTTCCCGATATTGGTTCTGGATTAACTGCTTCCCGGGTGACAGCTTCTCAGGTTCCTGATTATTTGGTGAATCCTTCAAAGTATACGTGTTACAGTTTTGATTCAACAAGTGAGGTTGATGATGGTGGCAACACCAAGGCTTGTATGGACTACATTACTCAGGTTGAAAGCTCAGGGTCAGAGATGGAGATGGAGGATGCTTCACCTCAACTTCCCGAGTCTGTGACTTTTGTTCCAAAGAAAAAGACTGTCGATGGTAGCACAAGAACAAAGCAGGACGAGGAAGATGATGATAAGCAGTCCTTAAATATAAAAGGCGTCGCTGTTGGCATTGCAGCTATGGAAGAAAATGAAATCAGTGCGGCAGAGGAGGAAGACAAGTCAGAGCAAAATGCAGCCGTTTTAAGTGATTGTTTATCAAAAAGTGGTCGGAAGTTTCGAACCAAACCAATGTCAGATGATGAGTCTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TSSC4

Protein Analysis

369

Amino Acids

40.14

Weight (kDa)

4.49

Isoelectric Point (pI)

56.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TSSC4 PF15264 222 - 278 7.9e-07 Tumour suppressing sub-chromosomal transferable candidate 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 107
AccI GTMKAC 1 cut(s) 719
AccII CGCG 2 cut(s) 138, 376
AciI CCGC 5 cut(s) 26, 80, 124, 504, 998
AclWI GGATC 3 cut(s) 36, 49, 214
AcsI RAATTY 1 cut(s) 194
AcuI CTGAAG 3 cut(s) 303, 444, 516
AcyI GRCGYC 3 cut(s) 108, 147, 954
AfiI CCNNNNNNNGG 7 cut(s) 93, 156, 157, 232, 402, 500, 647
AflIII ACRYGT 2 cut(s) 565, 722
AgsI TTSAA 5 cut(s) 343, 629, 714, 741, 803
AhlI ACTAGT 1 cut(s) 466
AjnI CCWGG 3 cut(s) 92, 387, 395
AluBI AGCT 7 cut(s) 12, 287, 428, 633, 680, 807, 974
AluI AGCT 7 cut(s) 12, 287, 428, 633, 680, 807, 974
Alw26I GTCTC 1 cut(s) 72
AlwI GGATC 3 cut(s) 36, 49, 214
Ama87I CYCGRG 2 cut(s) 669, 851
ApeKI GCWGC 4 cut(s) 392, 461, 971, 1031
ApoI RAATTY 1 cut(s) 194
ArsI GACNNNNNNTTYG 4 cut(s) 20, 52, 569, 601
AscI GGCGCGCC 1 cut(s) 136
AspLEI GCGC 4 cut(s) 110, 123, 138, 140
AspS9I GGNCC 2 cut(s) 96, 223
AsuC2I CCSGG 4 cut(s) 23, 158, 670, 671
AsuHPI GGTGA 7 cut(s) 203, 377, 483, 617, 685, 715, 831
AsuII TTCGAA 1 cut(s) 1075
AvaI CYCGRG 2 cut(s) 669, 851
AvaII GGWCC 2 cut(s) 96, 223
BamHI GGATCC 1 cut(s) 41
BanI GGYRCC 1 cut(s) 107
BbsI GAAGAC 1 cut(s) 1017
BbvI GCAGC 4 cut(s) 379, 448, 983, 1043
BccI CCATC 7 cut(s) 221, 494, 596, 752, 814, 820, 884
BceAI ACGGC 1 cut(s) 1019
BciT130I CCWGG 3 cut(s) 94, 389, 397
BcnI CCSGG 4 cut(s) 23, 158, 670, 671
BcoDI GTCTC 1 cut(s) 72
BcuI ACTAGT 1 cut(s) 466
BfaI CTAG 2 cut(s) 438, 467
BfoI RGCGCY 2 cut(s) 111, 124
BisI GCNGC 6 cut(s) 124, 393, 462, 972, 999, 1032
BlsI GCNGC 6 cut(s) 125, 394, 463, 973, 1000, 1033
Bme1390I CCNGG 7 cut(s) 23, 94, 158, 389, 397, 670, 671
Bme18I GGWCC 2 cut(s) 96, 223
BmeT110I CYCGRG 2 cut(s) 669, 851
BmgT120I GGNCC 2 cut(s) 96, 223
BmiI GGNNCC 5 cut(s) 43, 109, 225, 639, 690
BmrFI CCNGG 7 cut(s) 23, 94, 158, 389, 397, 670, 671
BmsI GCATC 2 cut(s) 409, 823
BpiI GAAGAC 1 cut(s) 1017
Bpu10I CCTNAGC 1 cut(s) 808
Bpu14I TTCGAA 1 cut(s) 1075
BpuMI CCSGG 4 cut(s) 23, 158, 670, 671
BsaAI YACGTR 1 cut(s) 723
BsaBI GATNNNNATC 1 cut(s) 297
BsaHI GRCGYC 3 cut(s) 108, 147, 954
BsaJI CCNNGG 5 cut(s) 92, 111, 328, 669, 771
BsaXI ACNNNNNCTCC 2 cut(s) 527, 557
Bsc4I CCNNNNNNNGG 7 cut(s) 93, 156, 157, 232, 402, 500, 647
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 1 cut(s) 449
Bse3DI GCAATG 1 cut(s) 966
Bse8I GATNNNNATC 1 cut(s) 297
BseBI CCWGG 3 cut(s) 94, 389, 397
BseDI CCNNGG 5 cut(s) 92, 111, 328, 669, 771
BseGI GGATG 1 cut(s) 838
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 7 cut(s) 93, 156, 157, 232, 402, 500, 647
BseMI GCAATG 1 cut(s) 966
BseMII CTCAG 4 cut(s) 443, 698, 809, 822
BseNI ACTGG 1 cut(s) 449
BsePI GCGCGC 1 cut(s) 136
BseRI GAGGAG 2 cut(s) 52, 1019
BseXI GCAGC 4 cut(s) 379, 448, 983, 1043
BsgI GTGCAG 1 cut(s) 447
Bsh1236I CGCG 2 cut(s) 138, 376
BshNI GGYRCC 1 cut(s) 107
BsiHKCI CYCGRG 2 cut(s) 669, 851
BsiSI CCGG 4 cut(s) 22, 105, 157, 670
BslFI GGGAC 2 cut(s) 173, 541
BslI CCNNNNNNNGG 7 cut(s) 93, 156, 157, 232, 402, 500, 647
BsmAI GTCTC 1 cut(s) 72
BsmBI CGTCTC 1 cut(s) 72
BsmFI GGGAC 2 cut(s) 173, 541
BsoBI CYCGRG 2 cut(s) 669, 851
Bsp119I TTCGAA 1 cut(s) 1075
Bsp143I GATC 4 cut(s) 41, 206, 298, 592
Bsp19I CCATGG 1 cut(s) 328
BspACI CCGC 5 cut(s) 26, 80, 124, 504, 998
BspCNI CTCAG 4 cut(s) 442, 697, 808, 821
BspFNI CGCG 2 cut(s) 138, 376
BspLI GGNNCC 5 cut(s) 43, 109, 225, 639, 690
BspPI GGATC 3 cut(s) 36, 49, 214
BspT104I TTCGAA 1 cut(s) 1075
BspT107I GGYRCC 1 cut(s) 107
BsrDI GCAATG 1 cut(s) 966
BsrFI RCCGGY 1 cut(s) 104
BsrI ACTGG 1 cut(s) 449
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 5 cut(s) 92, 111, 328, 669, 771
BssHII GCGCGC 1 cut(s) 136
BssMI GATC 4 cut(s) 41, 206, 298, 592
BssNAI GTATAC 1 cut(s) 720
BssNI GRCGYC 3 cut(s) 108, 147, 954
BssT1I CCWWGG 2 cut(s) 328, 771
Bst1107I GTATAC 1 cut(s) 720
Bst2UI CCWGG 3 cut(s) 94, 389, 397
Bst4CI ACNGT 3 cut(s) 480, 731, 886
Bst6I CTCTTC 1 cut(s) 240
BstACI GRCGYC 3 cut(s) 108, 147, 954
BstBAI YACGTR 1 cut(s) 723
BstBI TTCGAA 1 cut(s) 1075
BstC8I GCNNGC 3 cut(s) 106, 138, 378
BstDEI CTNAG 4 cut(s) 429, 684, 795, 808
BstDSI CCRYGG 1 cut(s) 328
BstF5I GGATG 1 cut(s) 838
BstFNI CGCG 2 cut(s) 138, 376
BstH2I RGCGCY 2 cut(s) 111, 124
BstHHI GCGC 4 cut(s) 110, 123, 138, 140
BstKTI GATC 4 cut(s) 44, 209, 301, 595
BstMAI GTCTC 1 cut(s) 72
BstMBI GATC 4 cut(s) 41, 206, 298, 592
BstMWI GCNNNNNNNGC 3 cut(s) 146, 278, 461
BstNI CCWGG 3 cut(s) 94, 389, 397
BstSCI CCNGG 7 cut(s) 21, 92, 156, 387, 395, 668, 669
BstUI CGCG 2 cut(s) 138, 376
BstV1I GCAGC 4 cut(s) 379, 448, 983, 1043
BstV2I GAAGAC 1 cut(s) 1017
BstX2I RGATCY 2 cut(s) 41, 206
BstXI CCANNNNNNTGG 1 cut(s) 220
BstYI RGATCY 2 cut(s) 41, 206
BstZ17I GTATAC 1 cut(s) 720
BtgI CCRYGG 1 cut(s) 328
BtsCI GGATG 1 cut(s) 838
BtsIMutI CAGTG 2 cut(s) 456, 1000
Cac8I GCNNGC 3 cut(s) 106, 138, 378
CfoI GCGC 4 cut(s) 110, 123, 138, 140
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 2 cut(s) 96, 223
Cfr9I CCCGGG 1 cut(s) 669
CseI GACGC 4 cut(s) 42, 155, 165, 943
CviAII CATG 2 cut(s) 220, 329
DdeI CTNAG 4 cut(s) 429, 684, 795, 808
DinI GGCGCC 1 cut(s) 109
DpnI GATC 4 cut(s) 43, 208, 300, 594
DpnII GATC 4 cut(s) 41, 206, 298, 592
Eam1104I CTCTTC 1 cut(s) 240
EarI CTCTTC 1 cut(s) 240
EciI GGCGGA 1 cut(s) 41
Eco130I CCWWGG 2 cut(s) 328, 771
Eco47I GGWCC 2 cut(s) 96, 223
Eco57I CTGAAG 3 cut(s) 303, 444, 516
Eco88I CYCGRG 2 cut(s) 669, 851
EcoRII CCWGG 3 cut(s) 92, 387, 395
EcoT14I CCWWGG 2 cut(s) 328, 771
EgeI GGCGCC 1 cut(s) 109
EheI GGCGCC 1 cut(s) 109
ErhI CCWWGG 2 cut(s) 328, 771
Esp3I CGTCTC 1 cut(s) 72
FaeI CATG 2 cut(s) 223, 332
FaiI YATR 9 cut(s) 221, 261, 311, 330, 521, 720, 782, 947, 977
FalI AAGNNNNNCTT 2 cut(s) 923, 955
FaqI GGGAC 2 cut(s) 173, 541
FatI CATG 2 cut(s) 219, 328
FblI GTMKAC 1 cut(s) 719
Fnu4HI GCNGC 6 cut(s) 124, 393, 462, 972, 999, 1032
FokI GGATG 1 cut(s) 845
Fsp4HI GCNGC 6 cut(s) 124, 393, 462, 972, 999, 1032
FspBI CTAG 2 cut(s) 438, 467
GlaI GCGC 4 cut(s) 109, 122, 137, 139
GluI GCNGC 6 cut(s) 124, 393, 462, 972, 999, 1032
HaeII RGCGCY 2 cut(s) 111, 124
HapII CCGG 4 cut(s) 22, 105, 157, 670
HgaI GACGC 4 cut(s) 42, 155, 165, 943
HhaI GCGC 4 cut(s) 110, 123, 138, 140
Hin1I GRCGYC 3 cut(s) 108, 147, 954
Hin1II CATG 2 cut(s) 223, 332
Hin6I GCGC 4 cut(s) 108, 121, 136, 138
HinP1I GCGC 4 cut(s) 108, 121, 136, 138
HindIII AAGCTT 1 cut(s) 631
HinfI GANTC 9 cut(s) 18, 201, 371, 382, 611, 706, 737, 854, 1100
HpaII CCGG 4 cut(s) 22, 105, 157, 670
HphI GGTGA 7 cut(s) 203, 377, 483, 617, 685, 715, 831
Hpy166II GTNNAC 1 cut(s) 720
Hpy188III TCNNGA 7 cut(s) 233, 558, 618, 641, 654, 692, 851
Hpy8I GTNNAC 1 cut(s) 720
Hpy99I CGWCG 6 cut(s) 58, 104, 149, 181, 184, 959
HpyAV CCTTC 1 cut(s) 720
HpyCH4III ACNGT 3 cut(s) 480, 731, 886
HpyCH4IV ACGT 2 cut(s) 565, 722
HpyCH4V TGCA 3 cut(s) 464, 971, 1031
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 278, 461
HpyF3I CTNAG 4 cut(s) 429, 684, 795, 808
HpySE526I ACGT 2 cut(s) 565, 722
Hsp92I GRCGYC 3 cut(s) 108, 147, 954
Hsp92II CATG 2 cut(s) 223, 332
HspAI GCGC 4 cut(s) 108, 121, 136, 138
KasI GGCGCC 1 cut(s) 107
KroI GCCGGC 1 cut(s) 104
KroNI GCCGGC 1 cut(s) 106
Kzo9I GATC 4 cut(s) 41, 206, 298, 592
Lsp1109I GCAGC 4 cut(s) 379, 448, 983, 1043
LweI GCATC 2 cut(s) 409, 823
MaeI CTAG 2 cut(s) 438, 467
MaeII ACGT 2 cut(s) 565, 722
MaeIII GTNAC 4 cut(s) 319, 673, 725, 859
MalI GATC 4 cut(s) 43, 208, 300, 594
MboI GATC 4 cut(s) 41, 206, 298, 592
MboII GAAGA 9 cut(s) 201, 257, 263, 425, 509, 620, 932, 992, 1022
MflI RGATCY 2 cut(s) 41, 206
MluCI AATT 2 cut(s) 194, 538
Mly113I GGCGCC 1 cut(s) 108
MlyI GAGTC 3 cut(s) 12, 863, 1109
MmeI TCCRAC 2 cut(s) 97, 1046
MreI CGCCGGCG 1 cut(s) 104
MroNI GCCGGC 1 cut(s) 104
MseI TTAA 5 cut(s) 471, 659, 941, 1040, 1108
MslI CAYNNNNRTG 1 cut(s) 218
MspI CCGG 4 cut(s) 22, 105, 157, 670
MspR9I CCNGG 7 cut(s) 23, 94, 158, 389, 397, 670, 671
MvaI CCWGG 3 cut(s) 94, 389, 397
MvnI CGCG 2 cut(s) 138, 376
MwoI GCNNNNNNNGC 3 cut(s) 146, 278, 461
NaeI GCCGGC 1 cut(s) 106
NarI GGCGCC 1 cut(s) 108
NciI CCSGG 4 cut(s) 23, 158, 670, 671
NcoI CCATGG 1 cut(s) 328
NdeII GATC 4 cut(s) 41, 206, 298, 592
NgoMIV GCCGGC 1 cut(s) 104
NlaIII CATG 2 cut(s) 223, 332
NlaIV GGNNCC 5 cut(s) 43, 109, 225, 639, 690
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 2 cut(s) 673, 859
NspV TTCGAA 1 cut(s) 1075
PalAI GGCGCGCC 1 cut(s) 136
PauI GCGCGC 1 cut(s) 136
PcsI WCGNNNNNNNCGW 4 cut(s) 62, 108, 173, 1072
PdiI GCCGGC 1 cut(s) 106
PfeI GAWTC 6 cut(s) 201, 371, 382, 611, 706, 737
PkrI GCNGC 6 cut(s) 125, 394, 463, 973, 1000, 1033
PleI GAGTC 3 cut(s) 12, 862, 1108
PluTI GGCGCC 1 cut(s) 111
PpsI GAGTC 3 cut(s) 12, 862, 1108
Ppu21I YACGTR 1 cut(s) 723
Psp6I CCWGG 3 cut(s) 92, 387, 395
PspGI CCWGG 3 cut(s) 92, 387, 395
PspN4I GGNNCC 5 cut(s) 43, 109, 225, 639, 690
PspPI GGNCC 2 cut(s) 96, 223
PsuI RGATCY 2 cut(s) 41, 206
PteI GCGCGC 1 cut(s) 136
RseI CAYNNNNRTG 1 cut(s) 218
SaqAI TTAA 5 cut(s) 471, 659, 941, 1040, 1108
SatI GCNGC 6 cut(s) 124, 393, 462, 972, 999, 1032
Sau3AI GATC 4 cut(s) 41, 206, 298, 592
Sau96I GGNCC 2 cut(s) 96, 223
SchI GAGTC 3 cut(s) 12, 863, 1109
ScrFI CCNGG 7 cut(s) 23, 94, 158, 389, 397, 670, 671
SfaNI GCATC 2 cut(s) 409, 823
SfoI GGCGCC 1 cut(s) 109
SfuI TTCGAA 1 cut(s) 1075
SgrAI CRCCGGYG 1 cut(s) 104
SgsI GGCGCGCC 1 cut(s) 136
SinI GGWCC 2 cut(s) 96, 223
SmaI CCCGGG 1 cut(s) 671
SmiMI CAYNNNNRTG 1 cut(s) 218
SpeI ACTAGT 1 cut(s) 466
Sse9I AATT 2 cut(s) 194, 538
SsiI CCGC 5 cut(s) 26, 80, 124, 504, 998
SspDI GGCGCC 1 cut(s) 107
SspMI CTAG 2 cut(s) 438, 467
StyD4I CCNGG 7 cut(s) 21, 92, 156, 387, 395, 668, 669
StyI CCWWGG 2 cut(s) 328, 771
TaaI ACNGT 3 cut(s) 480, 731, 886
TaiI ACGT 2 cut(s) 568, 725
TaqI TCGA 4 cut(s) 117, 240, 888, 1075
TasI AATT 2 cut(s) 194, 538
TauI GCSGC 2 cut(s) 126, 1001
TfiI GAWTC 6 cut(s) 201, 371, 382, 611, 706, 737
Tru1I TTAA 5 cut(s) 471, 659, 941, 1040, 1108
Tru9I TTAA 5 cut(s) 471, 659, 941, 1040, 1108
TscAI CASTG 2 cut(s) 456, 1000
TseFI GTSAC 2 cut(s) 673, 859
TseI GCWGC 4 cut(s) 392, 461, 971, 1031
Tsp45I GTSAC 2 cut(s) 673, 859
TspDTI ATGAA 2 cut(s) 270, 1002
TspGWI ACGGA 1 cut(s) 88
TspMI CCCGGG 1 cut(s) 669
TspRI CASTG 2 cut(s) 456, 1000
VpaK11BI GGWCC 2 cut(s) 96, 223
XapI RAATTY 1 cut(s) 194
XcmI CCANNNNNNNNNTGG 1 cut(s) 778
XmaI CCCGGG 1 cut(s) 669
XmiI GTMKAC 1 cut(s) 719
XspI CTAG 2 cut(s) 438, 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.