MD13G1129900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005010
Physical Location & Seq
Forward (+)
9838673 .. 9840551
1879 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1129900.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGGGCGACAGCTTCAGAGTTCGCGCGGACAAGGTTTTTGGATCGATCCCTTCGTCGACCTCGTCGTTCCCGAAAGTACAGCAATCCTCGCCCTGGTCCGTTACCGGAGAAGCGGTCGAGCGCAGAGAATGGAAGCGGGGCAAGGACGCCGGTACCTCCTCCAACAGAGACCAAACGCCGTGCTCGTCGTCGTTTCTGGAGGACGAATGGGGGATCAAAGCGTCGATCGGCAAGGACCCCACTCTGGATTTCGAGGAAGAGGAAGATGAATATGACAAAGTGGCGGCGGGAACAGAAGGCATAAGCGACCGTTTGTACATGGATGAGATTGCCAATCATGGGCCCTTTTTAAATTCTCAAAATGTGCTTCATGGTAGCAACAAAGATCCGCGTGCCAATTTCCTGGCTGCTAAACTTCGGCTGAAAGAAGATGATGCTGAAGCTCGAAAGTCGAATTCTACCCTGGCTGCTGCTTTACCAGTCAAGAAACAGACTGTAGAATCACCTGAAGACGGTGGCCGGCCAAAGTCTATATTGAAAAGGAAAGGCTATGCCTCAGATACGAAGGTGCAGAAACGTCCAAGCCCTTTCTCTATTAAAGCAAAAAGCAAGCTCGCAAAATACCGTCAGGTACCTGATTATTTGGTGAATCCTTCAAAGTATACATATTATAGCTTCGATACAACAGCCAAAGTCGATGAAGTTGCCAACGCCCAGGCTTGTACAGAGTACCTTACTCCGGATGAGAGTTCAAATTCAGAGTCAAGGTCAGAGACAGAGGATTCTTCACCTAAACTTCCTGTGTCTGTGACCTTTGTTTCAAAGAAGAAGAGAGGGGATGACAATGCAGTTAATGATAGCAAAAAAATGAAGTTAGACAAGGAAGATGACAAAACGGAGTCCTTAAAACAAGCAGGCATCCCTGTTGGCATTGCAGCCGTTGAAGTCCAAAGTGATGTTCGTGCGGTCGAGGAAGAAGGTTCGCAACCAAGTGCACCAGTTGCAAGTGTTTGTGTACAGAAATCTGGACGGAAGTATCGGAAAAAAACAGTATCTGATGAGTCTGATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TSSC4

Protein Analysis

358

Amino Acids

39.22

Weight (kDa)

6.0

Isoelectric Point (pI)

52.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TSSC4 PF15264 188 - 261 5.4e-07 Tumour suppressing sub-chromosomal transferable candidate 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 152, 631
AccB1I GGYRCC 2 cut(s) 152, 631
AccI GTMKAC 2 cut(s) 56, 662
AccII CGCG 3 cut(s) 24, 26, 391
AccIII TCCGGA 1 cut(s) 739
AciI CCGC 7 cut(s) 26, 113, 136, 284, 287, 389, 965
AclWI GGATC 4 cut(s) 40, 49, 221, 380
AcoI YGGCCR 2 cut(s) 517, 521
AcsI RAATTY 3 cut(s) 352, 454, 754
AcuI CTGAAG 2 cut(s) 459, 528
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 7 cut(s) 78, 154, 317, 633, 724, 731, 1017
AfiI CCNNNNNNNGG 5 cut(s) 93, 244, 339, 512, 739
AgsI TTSAA 5 cut(s) 538, 657, 753, 822, 944
AjnI CCWGG 4 cut(s) 92, 402, 462, 714
AjuI GAANNNNNNNTTGG 2 cut(s) 942, 974
AluBI AGCT 4 cut(s) 12, 443, 613, 675
AluI AGCT 4 cut(s) 12, 443, 613, 675
Alw21I GWGCWC 2 cut(s) 185, 997
Alw26I GTCTC 2 cut(s) 162, 767
Alw44I GTGCAC 1 cut(s) 993
AlwI GGATC 4 cut(s) 40, 49, 221, 380
AlwNI CAGNNNCTG 1 cut(s) 1055
Aor13HI TCCGGA 1 cut(s) 739
AoxI GGCC 3 cut(s) 341, 517, 521
ApaI GGGCCC 1 cut(s) 345
ApaLI GTGCAC 1 cut(s) 993
ApeKI GCWGC 4 cut(s) 407, 467, 470, 935
ApoI RAATTY 3 cut(s) 352, 454, 754
ArsI GACNNNNNNTTYG 4 cut(s) 20, 52, 746, 778
Asp718I GGTACC 2 cut(s) 152, 631
AspLEI GCGC 2 cut(s) 26, 123
AspS9I GGNCC 4 cut(s) 96, 235, 341, 342
AsuHPI GGTGA 3 cut(s) 495, 658, 780
AvaII GGWCC 2 cut(s) 96, 235
BaeGI GKGCMC 2 cut(s) 345, 997
BaeI ACNNNNGTAYC 6 cut(s) 136, 169, 552, 585, 1019, 1052
BanI GGYRCC 2 cut(s) 152, 631
BanII GRGCYC 1 cut(s) 345
BbsI GAAGAC 1 cut(s) 516
Bbv12I GWGCWC 2 cut(s) 185, 997
BbvI GCAGC 4 cut(s) 394, 454, 457, 947
BceAI ACGGC 2 cut(s) 163, 923
BcgI CGANNNNNNTGC 2 cut(s) 686, 720
BciT130I CCWGG 4 cut(s) 94, 404, 464, 716
BcoDI GTCTC 2 cut(s) 162, 767
BfmI CTRYAG 1 cut(s) 495
BisI GCNGC 5 cut(s) 285, 408, 468, 471, 936
BlsI GCNGC 5 cut(s) 286, 409, 469, 472, 937
Bme1390I CCNGG 4 cut(s) 94, 404, 464, 716
Bme18I GGWCC 2 cut(s) 96, 235
BmgT120I GGNCC 4 cut(s) 96, 235, 341, 342
BmiI GGNNCC 4 cut(s) 154, 237, 343, 633
BmrFI CCNGG 4 cut(s) 94, 404, 464, 716
BmsI GCATC 2 cut(s) 424, 927
BpiI GAAGAC 1 cut(s) 516
BpmI CTGGAG 1 cut(s) 218
Bsa29I ATCGAT 1 cut(s) 44
BsaHI GRCGYC 1 cut(s) 147
BsaI GGTCTC 1 cut(s) 162
BsaJI CCNNGG 3 cut(s) 92, 462, 714
BsaWI WCCGGW 2 cut(s) 104, 739
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
Bsc4I CCNNNNNNNGG 5 cut(s) 93, 244, 339, 512, 739
Bse118I RCCGGY 2 cut(s) 149, 519
Bse1I ACTGG 2 cut(s) 479, 998
Bse3DI GCAATG 1 cut(s) 930
BseAI TCCGGA 1 cut(s) 739
BseBI CCWGG 4 cut(s) 94, 404, 464, 716
BseCI ATCGAT 1 cut(s) 44
BseDI CCNNGG 3 cut(s) 92, 462, 714
BseGI GGATG 4 cut(s) 328, 748, 844, 918
BseLI CCNNNNNNNGG 5 cut(s) 93, 244, 339, 512, 739
BseMI GCAATG 1 cut(s) 930
BseMII CTCAG 1 cut(s) 570
BseNI ACTGG 2 cut(s) 479, 998
BseRI GAGGAG 1 cut(s) 148
BseSI GKGCMC 2 cut(s) 345, 997
BseXI GCAGC 4 cut(s) 394, 454, 457, 947
BsgI GTGCAG 1 cut(s) 590
Bsh1236I CGCG 3 cut(s) 24, 26, 391
Bsh1285I CGRYCG 4 cut(s) 117, 228, 310, 969
BshFI GGCC 3 cut(s) 343, 519, 523
BshNI GGYRCC 2 cut(s) 152, 631
BshVI ATCGAT 1 cut(s) 44
BsiEI CGRYCG 4 cut(s) 117, 228, 310, 969
BsiHKAI GWGCWC 2 cut(s) 185, 997
BsiSI CCGG 4 cut(s) 105, 150, 520, 740
BslI CCNNNNNNNGG 5 cut(s) 93, 244, 339, 512, 739
BsmAI GTCTC 2 cut(s) 162, 767
BsnI GGCC 3 cut(s) 343, 519, 523
Bso31I GGTCTC 1 cut(s) 162
Bsp120I GGGCCC 1 cut(s) 341
Bsp1286I GDGCHC 3 cut(s) 185, 345, 997
Bsp13I TCCGGA 1 cut(s) 739
Bsp1407I TGTACA 3 cut(s) 315, 722, 1015
Bsp143I GATC 5 cut(s) 41, 45, 213, 225, 385
BspACI CCGC 7 cut(s) 26, 113, 136, 284, 287, 389, 965
BspANI GGCC 3 cut(s) 343, 519, 523
BspCNI CTCAG 1 cut(s) 569
BspDI ATCGAT 1 cut(s) 44
BspEI TCCGGA 1 cut(s) 739
BspFNI CGCG 3 cut(s) 24, 26, 391
BspLI GGNNCC 4 cut(s) 154, 237, 343, 633
BspPI GGATC 4 cut(s) 40, 49, 221, 380
BspT107I GGYRCC 2 cut(s) 152, 631
BspTNI GGTCTC 1 cut(s) 162
BsrDI GCAATG 1 cut(s) 930
BsrFI RCCGGY 2 cut(s) 149, 519
BsrGI TGTACA 3 cut(s) 315, 722, 1015
BsrI ACTGG 2 cut(s) 479, 998
BssAI RCCGGY 2 cut(s) 149, 519
BssECI CCNNGG 3 cut(s) 92, 462, 714
BssMI GATC 5 cut(s) 41, 45, 213, 225, 385
BssNAI GTATAC 1 cut(s) 663
BssNI GRCGYC 1 cut(s) 147
Bst1107I GTATAC 1 cut(s) 663
Bst2UI CCWGG 4 cut(s) 94, 404, 464, 716
Bst4CI ACNGT 5 cut(s) 311, 496, 515, 626, 1051
Bst6I CTCTTC 2 cut(s) 252, 824
BstACI GRCGYC 1 cut(s) 147
BstAPI GCANNNNNTGC 1 cut(s) 1001
BstAUI TGTACA 3 cut(s) 315, 722, 1015
BstC8I GCNNGC 5 cut(s) 393, 521, 611, 615, 916
BstDEI CTNAG 1 cut(s) 556
BstF5I GGATG 4 cut(s) 328, 748, 844, 918
BstFNI CGCG 3 cut(s) 24, 26, 391
BstHHI GCGC 2 cut(s) 26, 123
BstKTI GATC 5 cut(s) 44, 48, 216, 228, 388
BstMAI GTCTC 2 cut(s) 162, 767
BstMBI GATC 5 cut(s) 41, 45, 213, 225, 385
BstMCI CGRYCG 4 cut(s) 117, 228, 310, 969
BstMWI GCNNNNNNNGC 2 cut(s) 88, 1001
BstNI CCWGG 4 cut(s) 94, 404, 464, 716
BstSCI CCNGG 4 cut(s) 92, 402, 462, 714
BstSFI CTRYAG 1 cut(s) 495
BstSLI GKGCMC 2 cut(s) 345, 997
BstUI CGCG 3 cut(s) 24, 26, 391
BstV1I GCAGC 4 cut(s) 394, 454, 457, 947
BstV2I GAAGAC 1 cut(s) 516
BstX2I RGATCY 1 cut(s) 385
BstXI CCANNNNNNTGG 1 cut(s) 403
BstYI RGATCY 1 cut(s) 385
BstZ17I GTATAC 1 cut(s) 663
Bsu15I ATCGAT 1 cut(s) 44
BsuRI GGCC 3 cut(s) 343, 519, 523
BsuTUI ATCGAT 1 cut(s) 44
BtsCI GGATG 4 cut(s) 328, 748, 844, 918
Cac8I GCNNGC 5 cut(s) 393, 521, 611, 615, 916
CaiI CAGNNNCTG 1 cut(s) 1055
CfoI GCGC 2 cut(s) 26, 123
Cfr10I RCCGGY 2 cut(s) 149, 519
Cfr13I GGNCC 4 cut(s) 96, 235, 341, 342
ClaI ATCGAT 1 cut(s) 44
CseI GACGC 2 cut(s) 155, 210
Csp6I GTAC 7 cut(s) 77, 153, 316, 632, 723, 730, 1016
CviAII CATG 3 cut(s) 319, 338, 371
CviQI GTAC 7 cut(s) 77, 153, 316, 632, 723, 730, 1016
DdeI CTNAG 1 cut(s) 556
DpnI GATC 5 cut(s) 43, 47, 215, 227, 387
DpnII GATC 5 cut(s) 41, 45, 213, 225, 385
DraI TTTAAA 1 cut(s) 351
EaeI YGGCCR 2 cut(s) 517, 521
Eam1104I CTCTTC 2 cut(s) 252, 824
EarI CTCTTC 2 cut(s) 252, 824
Eco24I GRGCYC 1 cut(s) 345
Eco31I GGTCTC 1 cut(s) 162
Eco47I GGWCC 2 cut(s) 96, 235
Eco57I CTGAAG 2 cut(s) 459, 528
EcoO109I RGGNCCY 2 cut(s) 235, 342
EcoRI GAATTC 1 cut(s) 454
EcoRII CCWGG 4 cut(s) 92, 402, 462, 714
EcoT38I GRGCYC 1 cut(s) 345
FaeI CATG 3 cut(s) 322, 341, 374
FatI CATG 3 cut(s) 318, 337, 370
FauI CCCGC 2 cut(s) 129, 280
FblI GTMKAC 2 cut(s) 56, 662
Fnu4HI GCNGC 5 cut(s) 285, 408, 468, 471, 936
FokI GGATG 4 cut(s) 335, 755, 851, 905
FriOI GRGCYC 1 cut(s) 345
FseI GGCCGGCC 1 cut(s) 523
Fsp4HI GCNGC 5 cut(s) 285, 408, 468, 471, 936
GlaI GCGC 2 cut(s) 25, 122
GluI GCNGC 5 cut(s) 285, 408, 468, 471, 936
GsuI CTGGAG 1 cut(s) 218
HaeIII GGCC 3 cut(s) 343, 519, 523
HapII CCGG 4 cut(s) 105, 150, 520, 740
HgaI GACGC 2 cut(s) 155, 210
HhaI GCGC 2 cut(s) 26, 123
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 3 cut(s) 322, 341, 374
Hin6I GCGC 2 cut(s) 24, 121
HinP1I GCGC 2 cut(s) 24, 121
HincII GTYRAC 1 cut(s) 57
HindII GTYRAC 1 cut(s) 57
HinfI GANTC 7 cut(s) 500, 649, 761, 782, 899, 1061, 1067
HpaII CCGG 4 cut(s) 105, 150, 520, 740
HphI GGTGA 3 cut(s) 495, 658, 780
Hpy166II GTNNAC 4 cut(s) 57, 663, 995, 1016
Hpy188I TCNGA 7 cut(s) 17, 559, 760, 772, 1041, 1057, 1066
Hpy188III TCNNGA 7 cut(s) 70, 197, 245, 484, 740, 1026, 1071
Hpy8I GTNNAC 4 cut(s) 57, 663, 995, 1016
Hpy99I CGWCG 5 cut(s) 58, 67, 190, 193, 226
HpyAV CCTTC 5 cut(s) 60, 290, 559, 663, 971
HpyCH4III ACNGT 5 cut(s) 311, 496, 515, 626, 1051
HpyCH4IV ACGT 1 cut(s) 577
HpyCH4V TGCA 5 cut(s) 571, 848, 935, 995, 1004
HpyF10VI GCNNNNNNNGC 2 cut(s) 88, 1001
HpyF3I CTNAG 1 cut(s) 556
HpySE526I ACGT 1 cut(s) 577
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 3 cut(s) 322, 341, 374
HspAI GCGC 2 cut(s) 24, 121
Kpn2I TCCGGA 1 cut(s) 739
KpnI GGTACC 2 cut(s) 156, 635
KroI GCCGGC 1 cut(s) 519
KroNI GCCGGC 1 cut(s) 521
Kzo9I GATC 5 cut(s) 41, 45, 213, 225, 385
Lsp1109I GCAGC 4 cut(s) 394, 454, 457, 947
LweI GCATC 2 cut(s) 424, 927
MaeII ACGT 1 cut(s) 577
MaeIII GTNAC 2 cut(s) 100, 808
MalI GATC 5 cut(s) 43, 47, 215, 227, 387
MboI GATC 5 cut(s) 41, 45, 213, 225, 385
MboII GAAGA 9 cut(s) 269, 275, 440, 521, 777, 838, 841, 896, 986
MflI RGATCY 1 cut(s) 385
MhlI GDGCHC 3 cut(s) 185, 345, 997
MluCI AATT 4 cut(s) 352, 397, 454, 754
MlyI GAGTC 3 cut(s) 770, 908, 1070
MmeI TCCRAC 1 cut(s) 186
MroI TCCGGA 1 cut(s) 739
MroNI GCCGGC 1 cut(s) 519
MseI TTAA 4 cut(s) 350, 597, 852, 905
MspI CCGG 4 cut(s) 105, 150, 520, 740
MspR9I CCNGG 4 cut(s) 94, 404, 464, 716
MvaI CCWGG 4 cut(s) 94, 404, 464, 716
MvnI CGCG 3 cut(s) 24, 26, 391
MwoI GCNNNNNNNGC 2 cut(s) 88, 1001
NaeI GCCGGC 1 cut(s) 521
NdeII GATC 5 cut(s) 41, 45, 213, 225, 385
NgoMIV GCCGGC 1 cut(s) 519
NlaIII CATG 3 cut(s) 322, 341, 374
NlaIV GGNNCC 4 cut(s) 154, 237, 343, 633
NmuCI GTSAC 1 cut(s) 808
PcsI WCGNNNNNNNCGW 5 cut(s) 50, 59, 62, 68, 182
PdiI GCCGGC 1 cut(s) 521
PfeI GAWTC 4 cut(s) 500, 649, 782, 1067
PflFI GACNNNGTC 1 cut(s) 61
PkrI GCNGC 5 cut(s) 286, 409, 469, 472, 937
Ple19I CGATCG 1 cut(s) 228
PleI GAGTC 3 cut(s) 769, 907, 1069
PpsI GAGTC 3 cut(s) 769, 907, 1069
PpuMI RGGWCCY 1 cut(s) 235
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 4 cut(s) 92, 402, 462, 714
PspGI CCWGG 4 cut(s) 92, 402, 462, 714
PspN4I GGNNCC 4 cut(s) 154, 237, 343, 633
PspOMI GGGCCC 1 cut(s) 341
PspPI GGNCC 4 cut(s) 96, 235, 341, 342
PspPPI RGGWCCY 1 cut(s) 235
PstNI CAGNNNCTG 1 cut(s) 1055
PsuI RGATCY 1 cut(s) 385
PsyI GACNNNGTC 1 cut(s) 61
PvuI CGATCG 1 cut(s) 228
RigI GGCCGGCC 1 cut(s) 523
RsaI GTAC 7 cut(s) 78, 154, 317, 633, 724, 731, 1017
RsaNI GTAC 7 cut(s) 77, 153, 316, 632, 723, 730, 1016
SalI GTCGAC 1 cut(s) 55
SaqAI TTAA 4 cut(s) 350, 597, 852, 905
SatI GCNGC 5 cut(s) 285, 408, 468, 471, 936
Sau3AI GATC 5 cut(s) 41, 45, 213, 225, 385
Sau96I GGNCC 4 cut(s) 96, 235, 341, 342
SchI GAGTC 3 cut(s) 770, 908, 1070
ScrFI CCNGG 4 cut(s) 94, 404, 464, 716
SduI GDGCHC 3 cut(s) 185, 345, 997
SfaNI GCATC 2 cut(s) 424, 927
SfcI CTRYAG 1 cut(s) 495
SinI GGWCC 2 cut(s) 96, 235
Sse9I AATT 4 cut(s) 352, 397, 454, 754
SsiI CCGC 7 cut(s) 26, 113, 136, 284, 287, 389, 965
StyD4I CCNGG 4 cut(s) 92, 402, 462, 714
TaaI ACNGT 5 cut(s) 311, 496, 515, 626, 1051
TaiI ACGT 1 cut(s) 580
TasI AATT 4 cut(s) 352, 397, 454, 754
TatI WGTACW 4 cut(s) 76, 315, 722, 1015
TauI GCSGC 1 cut(s) 287
TfiI GAWTC 4 cut(s) 500, 649, 782, 1067
Tru1I TTAA 4 cut(s) 350, 597, 852, 905
Tru9I TTAA 4 cut(s) 350, 597, 852, 905
TseFI GTSAC 1 cut(s) 808
TseI GCWGC 4 cut(s) 407, 467, 470, 935
Tsp45I GTSAC 1 cut(s) 808
TspDTI ATGAA 4 cut(s) 282, 359, 714, 884
TspGWI ACGGA 3 cut(s) 88, 911, 1045
Tth111I GACNNNGTC 1 cut(s) 61
VneI GTGCAC 1 cut(s) 993
VpaK11BI GGWCC 2 cut(s) 96, 235
XapI RAATTY 3 cut(s) 352, 454, 754
XmiI GTMKAC 2 cut(s) 56, 662
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.