Rroxscaffold_5G00370620

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005010
Physical Location & Seq
Reverse (-)
52308218 .. 52309718
1501 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00370620.1

Sequence Viewer

Length: 1125 bp
ATGGACGACAGCTTCAGACTCCGGGCGGACAAGGTTTTCGGATCCCTCTCTGCGTCGCTCTCCTCGTCTCTGCAACCACCGCAGTCCTCTCCCTGGTCCGTCGCCGGCGCCGAGGTCGAGCGCCGCCAATGGAGGCGCGCCACCGACGCCCCTGACCGAGACGAAACGCCCTGTGCGTCGTCGTTTCAGGGTGAATTTGGAATCGGATCTTCCATAGGCATGGACCCTACTCTGGATTTCGAGGAAGAGGAAGATGAATATGACAAAGTGGCTTCGGGTGCTGAAGCTGTTGATGATGATCGTTTGTTTATGGACGATGTTACCCACCATGACTCTTATTTAAACTCTTACAATGTATTGGTTGGTAAGGATTCGCGTGCGAATCACCTGGCCGCCAGGCACAGGCTGAAAGAAGATGATGCTGAAGCTCAGAACCCTAGTTCTACACCAGTGGCTTCTGCTGCACTGGTTAAGGAACAATTTGCGAAATCACCTGAAGATGGCGGTCAGCCAAAGCCTATACTGAAAAGGAAAGACAATTCCTCGGATAGCAAGTCCCGAAAACGTGTTAGGTTTGACCCTGCTTTTGTAGATCAGAGTGATGGTGAAGAATCATCAGGAAAAGTTCAAAACTTGGTTTCGGATAATGGTTCTGGATTAACTGCTTCCCGGGTTTCTTTGAAGAAAAAGACGGCTTCTCAGGTTCCTGATTATTTGGTGAATCCTTCAAAGTATACGTGTTATAGTTTTGATTCAACAAGTGAAGTTGATGAAGGTGGCAACACCAAGGCTTGTATGGACTACATTACTCAGGTTGAAAGCTCAGGGTCAGAGATGGAGATGGAGGATGCTTCACCTCAACTTCCCAAGACTGTGACTTTTGTTCCAAAGAAAAAGACTGTCGATGGTAGCACAAGAACAAAGCAGGACGAGGAAGATGATGATAAGCAGTCCTTAAATATAAAAGGCGTCGCTGTTGGCATTGCAGCTATGGAAGAAAATGAAATCAGTGCGGCAGAGGAGGAAGACAAGTCAGAGCAAAATGCAGCTGTTTTAAGTGATTGTTTATCAAAAAGTGGTCGGAAGTTTCGAACCAAGCCAATGTCAGATGATGAGTCTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TSSC4

Protein Analysis

374

Amino Acids

40.85

Weight (kDa)

4.62

Isoelectric Point (pI)

53.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TSSC4 PF15264 228 - 283 6e-07 Tumour suppressing sub-chromosomal transferable candidate 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 107
AccI GTMKAC 1 cut(s) 734
AccII CGCG 2 cut(s) 138, 376
AciI CCGC 6 cut(s) 26, 80, 124, 393, 504, 1013
AclWI GGATC 3 cut(s) 36, 49, 214
AcoI YGGCCR 1 cut(s) 390
AcsI RAATTY 1 cut(s) 194
AcuI CTGAAG 3 cut(s) 303, 444, 516
AcyI GRCGYC 3 cut(s) 108, 147, 969
AfiI CCNNNNNNNGG 4 cut(s) 93, 232, 402, 500
AflIII ACRYGT 2 cut(s) 565, 737
AgsI TTSAA 5 cut(s) 629, 682, 729, 756, 818
AjnI CCWGG 3 cut(s) 92, 387, 395
AluBI AGCT 6 cut(s) 12, 287, 428, 822, 989, 1049
AluI AGCT 6 cut(s) 12, 287, 428, 822, 989, 1049
Alw26I GTCTC 2 cut(s) 72, 153
AlwI GGATC 3 cut(s) 36, 49, 214
Ama87I CYCGRG 1 cut(s) 669
AoxI GGCC 1 cut(s) 390
ApeKI GCWGC 3 cut(s) 461, 986, 1046
ApoI RAATTY 1 cut(s) 194
ArsI GACNNNNNNTTYG 4 cut(s) 20, 52, 569, 601
AscI GGCGCGCC 1 cut(s) 136
AspLEI GCGC 4 cut(s) 110, 123, 138, 140
AspS9I GGNCC 2 cut(s) 96, 223
AsuC2I CCSGG 3 cut(s) 23, 670, 671
AsuHPI GGTGA 6 cut(s) 203, 377, 483, 617, 730, 846
AsuII TTCGAA 1 cut(s) 1090
AvaI CYCGRG 1 cut(s) 669
AvaII GGWCC 2 cut(s) 96, 223
BamHI GGATCC 1 cut(s) 41
BanI GGYRCC 1 cut(s) 107
BbsI GAAGAC 1 cut(s) 1032
BbvI GCAGC 3 cut(s) 448, 998, 1058
BccI CCATC 5 cut(s) 494, 596, 829, 835, 899
BceAI ACGGC 1 cut(s) 708
BciT130I CCWGG 3 cut(s) 94, 389, 397
BcnI CCSGG 3 cut(s) 23, 670, 671
BcoDI GTCTC 2 cut(s) 72, 153
BfaI CTAG 1 cut(s) 438
BfoI RGCGCY 2 cut(s) 111, 124
BisI GCNGC 6 cut(s) 124, 393, 462, 987, 1014, 1047
BlsI GCNGC 6 cut(s) 125, 394, 463, 988, 1015, 1048
Bme1390I CCNGG 6 cut(s) 23, 94, 389, 397, 670, 671
Bme18I GGWCC 2 cut(s) 96, 223
BmeT110I CYCGRG 1 cut(s) 669
BmgT120I GGNCC 2 cut(s) 96, 223
BmiI GGNNCC 4 cut(s) 43, 109, 225, 705
BmrFI CCNGG 6 cut(s) 23, 94, 389, 397, 670, 671
BmsI GCATC 2 cut(s) 409, 838
BpiI GAAGAC 1 cut(s) 1032
Bpu10I CCTNAGC 1 cut(s) 823
Bpu14I TTCGAA 1 cut(s) 1090
BpuMI CCSGG 3 cut(s) 23, 670, 671
BsaAI YACGTR 1 cut(s) 738
BsaBI GATNNNNATC 1 cut(s) 297
BsaHI GRCGYC 3 cut(s) 108, 147, 969
BsaJI CCNNGG 5 cut(s) 92, 111, 543, 669, 786
Bsc4I CCNNNNNNNGG 4 cut(s) 93, 232, 402, 500
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 449, 471
Bse3DI GCAATG 1 cut(s) 981
Bse8I GATNNNNATC 1 cut(s) 297
BseBI CCWGG 3 cut(s) 94, 389, 397
BseDI CCNNGG 5 cut(s) 92, 111, 543, 669, 786
BseGI GGATG 1 cut(s) 853
BseJI GATNNNNATC 1 cut(s) 297
BseLI CCNNNNNNNGG 4 cut(s) 93, 232, 402, 500
BseMI GCAATG 1 cut(s) 981
BseMII CTCAG 4 cut(s) 443, 713, 824, 837
BseNI ACTGG 2 cut(s) 449, 471
BsePI GCGCGC 1 cut(s) 136
BseRI GAGGAG 2 cut(s) 52, 1034
BseXI GCAGC 3 cut(s) 448, 998, 1058
BsgI GTGCAG 1 cut(s) 447
Bsh1236I CGCG 2 cut(s) 138, 376
BshFI GGCC 1 cut(s) 392
BshNI GGYRCC 1 cut(s) 107
BsiHKCI CYCGRG 1 cut(s) 669
BsiSI CCGG 3 cut(s) 22, 105, 670
BslFI GGGAC 1 cut(s) 541
BslI CCNNNNNNNGG 4 cut(s) 93, 232, 402, 500
BsmAI GTCTC 2 cut(s) 72, 153
BsmBI CGTCTC 2 cut(s) 72, 153
BsmFI GGGAC 1 cut(s) 541
BsnI GGCC 1 cut(s) 392
BsoBI CYCGRG 1 cut(s) 669
Bsp119I TTCGAA 1 cut(s) 1090
Bsp143I GATC 4 cut(s) 41, 206, 298, 592
BspACI CCGC 6 cut(s) 26, 80, 124, 393, 504, 1013
BspANI GGCC 1 cut(s) 392
BspCNI CTCAG 4 cut(s) 442, 712, 823, 836
BspFNI CGCG 2 cut(s) 138, 376
BspLI GGNNCC 4 cut(s) 43, 109, 225, 705
BspPI GGATC 3 cut(s) 36, 49, 214
BspT104I TTCGAA 1 cut(s) 1090
BspT107I GGYRCC 1 cut(s) 107
BsrDI GCAATG 1 cut(s) 981
BsrFI RCCGGY 1 cut(s) 104
BsrI ACTGG 2 cut(s) 449, 471
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 5 cut(s) 92, 111, 543, 669, 786
BssHII GCGCGC 1 cut(s) 136
BssMI GATC 4 cut(s) 41, 206, 298, 592
BssNAI GTATAC 1 cut(s) 735
BssNI GRCGYC 3 cut(s) 108, 147, 969
BssT1I CCWWGG 1 cut(s) 786
Bst1107I GTATAC 1 cut(s) 735
Bst2UI CCWGG 3 cut(s) 94, 389, 397
Bst4CI ACNGT 2 cut(s) 874, 901
Bst6I CTCTTC 1 cut(s) 240
BstACI GRCGYC 3 cut(s) 108, 147, 969
BstBAI YACGTR 1 cut(s) 738
BstBI TTCGAA 1 cut(s) 1090
BstC8I GCNNGC 3 cut(s) 106, 138, 378
BstDEI CTNAG 4 cut(s) 429, 699, 810, 823
BstF5I GGATG 1 cut(s) 853
BstFNI CGCG 2 cut(s) 138, 376
BstH2I RGCGCY 2 cut(s) 111, 124
BstHHI GCGC 4 cut(s) 110, 123, 138, 140
BstKTI GATC 4 cut(s) 44, 209, 301, 595
BstMAI GTCTC 2 cut(s) 72, 153
BstMBI GATC 4 cut(s) 41, 206, 298, 592
BstMWI GCNNNNNNNGC 4 cut(s) 79, 146, 278, 461
BstNI CCWGG 3 cut(s) 94, 389, 397
BstSCI CCNGG 6 cut(s) 21, 92, 387, 395, 668, 669
BstUI CGCG 2 cut(s) 138, 376
BstV1I GCAGC 3 cut(s) 448, 998, 1058
BstV2I GAAGAC 1 cut(s) 1032
BstX2I RGATCY 2 cut(s) 41, 206
BstXI CCANNNNNNTGG 1 cut(s) 220
BstYI RGATCY 2 cut(s) 41, 206
BstZ17I GTATAC 1 cut(s) 735
BsuRI GGCC 1 cut(s) 392
BtsCI GGATG 1 cut(s) 853
BtsIMutI CAGTG 3 cut(s) 456, 464, 1015
Cac8I GCNNGC 3 cut(s) 106, 138, 378
CfoI GCGC 4 cut(s) 110, 123, 138, 140
Cfr10I RCCGGY 1 cut(s) 104
Cfr13I GGNCC 2 cut(s) 96, 223
Cfr9I CCCGGG 1 cut(s) 669
CseI GACGC 4 cut(s) 42, 155, 165, 958
CviAII CATG 2 cut(s) 220, 329
DdeI CTNAG 4 cut(s) 429, 699, 810, 823
DinI GGCGCC 1 cut(s) 109
DpnI GATC 4 cut(s) 43, 208, 300, 594
DpnII GATC 4 cut(s) 41, 206, 298, 592
DraI TTTAAA 1 cut(s) 342
EaeI YGGCCR 1 cut(s) 390
Eam1104I CTCTTC 1 cut(s) 240
EarI CTCTTC 1 cut(s) 240
EciI GGCGGA 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 786
Eco47I GGWCC 2 cut(s) 96, 223
Eco57I CTGAAG 3 cut(s) 303, 444, 516
Eco88I CYCGRG 1 cut(s) 669
EcoRII CCWGG 3 cut(s) 92, 387, 395
EcoT14I CCWWGG 1 cut(s) 786
EgeI GGCGCC 1 cut(s) 109
EheI GGCGCC 1 cut(s) 109
ErhI CCWWGG 1 cut(s) 786
Esp3I CGTCTC 2 cut(s) 72, 153
FaeI CATG 2 cut(s) 223, 332
FalI AAGNNNNNCTT 2 cut(s) 938, 970
FaqI GGGAC 1 cut(s) 541
FatI CATG 2 cut(s) 219, 328
FblI GTMKAC 1 cut(s) 734
Fnu4HI GCNGC 6 cut(s) 124, 393, 462, 987, 1014, 1047
FokI GGATG 1 cut(s) 860
Fsp4HI GCNGC 6 cut(s) 124, 393, 462, 987, 1014, 1047
FspBI CTAG 1 cut(s) 438
GlaI GCGC 4 cut(s) 109, 122, 137, 139
GluI GCNGC 6 cut(s) 124, 393, 462, 987, 1014, 1047
HaeII RGCGCY 2 cut(s) 111, 124
HaeIII GGCC 1 cut(s) 392
HapII CCGG 3 cut(s) 22, 105, 670
HgaI GACGC 4 cut(s) 42, 155, 165, 958
HhaI GCGC 4 cut(s) 110, 123, 138, 140
Hin1I GRCGYC 3 cut(s) 108, 147, 969
Hin1II CATG 2 cut(s) 223, 332
Hin6I GCGC 4 cut(s) 108, 121, 136, 138
HinP1I GCGC 4 cut(s) 108, 121, 136, 138
HinfI GANTC 9 cut(s) 18, 201, 332, 371, 382, 611, 721, 752, 1115
HpaII CCGG 3 cut(s) 22, 105, 670
HphI GGTGA 6 cut(s) 203, 377, 483, 617, 730, 846
Hpy166II GTNNAC 1 cut(s) 735
Hpy188III TCNNGA 5 cut(s) 233, 558, 618, 654, 707
Hpy8I GTNNAC 1 cut(s) 735
Hpy99I CGWCG 6 cut(s) 58, 104, 149, 181, 184, 974
HpyAV CCTTC 2 cut(s) 735, 767
HpyCH4III ACNGT 2 cut(s) 874, 901
HpyCH4IV ACGT 2 cut(s) 565, 737
HpyCH4V TGCA 4 cut(s) 73, 464, 986, 1046
HpyF10VI GCNNNNNNNGC 4 cut(s) 79, 146, 278, 461
HpyF3I CTNAG 4 cut(s) 429, 699, 810, 823
HpySE526I ACGT 2 cut(s) 565, 737
Hsp92I GRCGYC 3 cut(s) 108, 147, 969
Hsp92II CATG 2 cut(s) 223, 332
HspAI GCGC 4 cut(s) 108, 121, 136, 138
KasI GGCGCC 1 cut(s) 107
KroI GCCGGC 1 cut(s) 104
KroNI GCCGGC 1 cut(s) 106
Kzo9I GATC 4 cut(s) 41, 206, 298, 592
Lsp1109I GCAGC 3 cut(s) 448, 998, 1058
LweI GCATC 2 cut(s) 409, 838
MaeI CTAG 1 cut(s) 438
MaeII ACGT 2 cut(s) 565, 737
MaeIII GTNAC 2 cut(s) 319, 874
MalI GATC 4 cut(s) 43, 208, 300, 594
MboI GATC 4 cut(s) 41, 206, 298, 592
MflI RGATCY 2 cut(s) 41, 206
MluCI AATT 3 cut(s) 194, 479, 538
Mly113I GGCGCC 1 cut(s) 108
MlyI GAGTC 3 cut(s) 12, 326, 1124
MmeI TCCRAC 1 cut(s) 1061
MreI CGCCGGCG 1 cut(s) 104
MroNI GCCGGC 1 cut(s) 104
MseI TTAA 6 cut(s) 341, 471, 659, 956, 1055, 1123
MslI CAYNNNNRTG 1 cut(s) 218
MspA1I CMGCKG 1 cut(s) 1049
MspI CCGG 3 cut(s) 22, 105, 670
MspR9I CCNGG 6 cut(s) 23, 94, 389, 397, 670, 671
MvaI CCWGG 3 cut(s) 94, 389, 397
MvnI CGCG 2 cut(s) 138, 376
MwoI GCNNNNNNNGC 4 cut(s) 79, 146, 278, 461
NaeI GCCGGC 1 cut(s) 106
NarI GGCGCC 1 cut(s) 108
NciI CCSGG 3 cut(s) 23, 670, 671
NdeII GATC 4 cut(s) 41, 206, 298, 592
NgoMIV GCCGGC 1 cut(s) 104
NlaIII CATG 2 cut(s) 223, 332
NlaIV GGNNCC 4 cut(s) 43, 109, 225, 705
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 1 cut(s) 874
NspV TTCGAA 1 cut(s) 1090
PalAI GGCGCGCC 1 cut(s) 136
PauI GCGCGC 1 cut(s) 136
PcsI WCGNNNNNNNCGW 4 cut(s) 62, 108, 173, 1087
PdiI GCCGGC 1 cut(s) 106
PfeI GAWTC 6 cut(s) 201, 371, 382, 611, 721, 752
PkrI GCNGC 6 cut(s) 125, 394, 463, 988, 1015, 1048
PleI GAGTC 3 cut(s) 12, 326, 1123
PluTI GGCGCC 1 cut(s) 111
PpsI GAGTC 3 cut(s) 12, 326, 1123
Ppu21I YACGTR 1 cut(s) 738
Psp6I CCWGG 3 cut(s) 92, 387, 395
PspGI CCWGG 3 cut(s) 92, 387, 395
PspN4I GGNNCC 4 cut(s) 43, 109, 225, 705
PspPI GGNCC 2 cut(s) 96, 223
PsuI RGATCY 2 cut(s) 41, 206
PteI GCGCGC 1 cut(s) 136
PvuII CAGCTG 1 cut(s) 1049
RseI CAYNNNNRTG 1 cut(s) 218
SaqAI TTAA 6 cut(s) 341, 471, 659, 956, 1055, 1123
SatI GCNGC 6 cut(s) 124, 393, 462, 987, 1014, 1047
Sau3AI GATC 4 cut(s) 41, 206, 298, 592
Sau96I GGNCC 2 cut(s) 96, 223
SchI GAGTC 3 cut(s) 12, 326, 1124
ScrFI CCNGG 6 cut(s) 23, 94, 389, 397, 670, 671
SfaNI GCATC 2 cut(s) 409, 838
SfoI GGCGCC 1 cut(s) 109
SfuI TTCGAA 1 cut(s) 1090
SgrAI CRCCGGYG 1 cut(s) 104
SgsI GGCGCGCC 1 cut(s) 136
SinI GGWCC 2 cut(s) 96, 223
SmaI CCCGGG 1 cut(s) 671
SmiMI CAYNNNNRTG 1 cut(s) 218
Sse9I AATT 3 cut(s) 194, 479, 538
SsiI CCGC 6 cut(s) 26, 80, 124, 393, 504, 1013
SspDI GGCGCC 1 cut(s) 107
SspMI CTAG 1 cut(s) 438
StyD4I CCNGG 6 cut(s) 21, 92, 387, 395, 668, 669
StyI CCWWGG 1 cut(s) 786
TaaI ACNGT 2 cut(s) 874, 901
TaiI ACGT 2 cut(s) 568, 740
TaqI TCGA 4 cut(s) 117, 240, 903, 1090
TaqII GACCGA 1 cut(s) 171
TasI AATT 3 cut(s) 194, 479, 538
TauI GCSGC 3 cut(s) 126, 395, 1016
TfiI GAWTC 6 cut(s) 201, 371, 382, 611, 721, 752
Tru1I TTAA 6 cut(s) 341, 471, 659, 956, 1055, 1123
Tru9I TTAA 6 cut(s) 341, 471, 659, 956, 1055, 1123
TscAI CASTG 3 cut(s) 456, 471, 1015
TseFI GTSAC 1 cut(s) 874
TseI GCWGC 3 cut(s) 461, 986, 1046
Tsp45I GTSAC 1 cut(s) 874
TspDTI ATGAA 3 cut(s) 270, 786, 1017
TspGWI ACGGA 1 cut(s) 88
TspMI CCCGGG 1 cut(s) 669
TspRI CASTG 3 cut(s) 456, 471, 1015
VpaK11BI GGWCC 2 cut(s) 96, 223
XapI RAATTY 1 cut(s) 194
XcmI CCANNNNNNNNNTGG 1 cut(s) 793
XmaI CCCGGG 1 cut(s) 669
XmiI GTMKAC 1 cut(s) 734
XspI CTAG 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.