FvH4_4g23501

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008854
Physical Location & Seq
Reverse (-)
25961508 .. 25964407
2900 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23501.t1

Sequence Viewer

Length: 498 bp
ATGGCAGCAGTTGCATCTCTTGTAGCCAACCCAGTTTTCATGCGCAATCATCGCCCGAAATTCAATGCAGCAAAATCGTGCATCGGTCGGCACGCGTCTGTTTCTTTTTCTTCTTCTGCTCGTAAATTCAACGTTGACGTGAAACTCAAAAGCTTCTTCTCCACAGCCGAGAACAACCAGTATTGCTCAACCATCAGCACCGACTCAATAGTATCAGGGAGGCATGCTTCTCTGTGTCCAACGCCTAATGCCATTAAAGATCACCTTGGTATTGGAAAACCACTTGGACGGGTCGGAGGGAAGTGGTCGACGGCGTATCCGCTTATACCTGATATTCTTTGGAAGTTCGATGGCTCTGAATATTTGGTGCGATGGAGTAGTTCGTGTTGTCAAGCTCCAGACGAGGAACATGCCTTGCAGAGCTATATTCAAGTATCAGTTACAACAACAGGCCTTTTTCCGGAGAAGGATTTGAAGGATATATCTGTTCTGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

166

Amino Acids

18.0

Weight (kDa)

9.02

Isoelectric Point (pI)

35.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 44
AccI GTMKAC 1 cut(s) 308
AccII CGCG 1 cut(s) 95
AccIII TCCGGA 1 cut(s) 460
AciI CCGC 1 cut(s) 320
AclI AACGTT 1 cut(s) 132
AcsI RAATTY 2 cut(s) 59, 125
AfiI CCNNNNNNNGG 1 cut(s) 460
AflIII ACRYGT 1 cut(s) 93
AgsI TTSAA 4 cut(s) 64, 130, 431, 475
AjiI CACGTC 1 cut(s) 139
AjuI GAANNNNNNNTTGG 2 cut(s) 20, 52
AluBI AGCT 3 cut(s) 153, 395, 423
AluI AGCT 3 cut(s) 153, 395, 423
Aor13HI TCCGGA 1 cut(s) 460
AoxI GGCC 1 cut(s) 451
ApeKI GCWGC 2 cut(s) 5, 68
ApoI RAATTY 2 cut(s) 59, 125
AspLEI GCGC 1 cut(s) 45
AsuHPI GGTGA 1 cut(s) 254
BbvI GCAGC 2 cut(s) 17, 80
BccI CCATC 3 cut(s) 200, 344, 366
BceAI ACGGC 1 cut(s) 327
BcgI CGANNNNNNTGC 4 cut(s) 57, 91, 392, 426
BciVI GTATCC 1 cut(s) 327
BfuI GTATCC 1 cut(s) 327
BisI GCNGC 2 cut(s) 6, 69
BlsI GCNGC 2 cut(s) 7, 70
BmgBI CACGTC 1 cut(s) 139
BmrI ACTGGG 1 cut(s) 26
BmsI GCATC 2 cut(s) 23, 90
BmuI ACTGGG 1 cut(s) 26
BpmI CTGGAG 1 cut(s) 381
BsaJI CCNNGG 1 cut(s) 265
BsaWI WCCGGW 1 cut(s) 460
Bsc4I CCNNNNNNNGG 1 cut(s) 460
Bse1I ACTGG 2 cut(s) 32, 178
BseAI TCCGGA 1 cut(s) 460
BseDI CCNNGG 1 cut(s) 265
BseLI CCNNNNNNNGG 1 cut(s) 460
BseNI ACTGG 2 cut(s) 32, 178
BseXI GCAGC 2 cut(s) 17, 80
Bsh1236I CGCG 1 cut(s) 95
Bsh1285I CGRYCG 1 cut(s) 88
BshFI GGCC 1 cut(s) 453
BsiEI CGRYCG 1 cut(s) 88
BsiSI CCGG 1 cut(s) 461
BslI CCNNNNNNNGG 1 cut(s) 460
BsnI GGCC 1 cut(s) 453
Bsp13I TCCGGA 1 cut(s) 460
Bsp143I GATC 1 cut(s) 259
BspACI CCGC 1 cut(s) 320
BspANI GGCC 1 cut(s) 453
BspEI TCCGGA 1 cut(s) 460
BspFNI CGCG 1 cut(s) 95
BsrI ACTGG 2 cut(s) 32, 178
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 1 cut(s) 259
BssT1I CCWWGG 1 cut(s) 265
BstAPI GCANNNNNTGC 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 93, 225
BstDEI CTNAG 1 cut(s) 495
BstFNI CGCG 1 cut(s) 95
BstHHI GCGC 1 cut(s) 45
BstKTI GATC 1 cut(s) 262
BstMBI GATC 1 cut(s) 259
BstMCI CGRYCG 1 cut(s) 88
BstMWI GCNNNNNNNGC 2 cut(s) 11, 51
BstNSI RCATGY 2 cut(s) 227, 413
BstUI CGCG 1 cut(s) 95
BstV1I GCAGC 2 cut(s) 17, 80
BsuI GTATCC 1 cut(s) 327
BsuRI GGCC 1 cut(s) 453
BtgZI GCGATG 2 cut(s) 35, 385
BtrI CACGTC 1 cut(s) 139
Cac8I GCNNGC 2 cut(s) 93, 225
CfoI GCGC 1 cut(s) 45
CseI GACGC 1 cut(s) 84
CviAII CATG 3 cut(s) 40, 224, 410
CviJI RGCY 8 cut(s) 26, 153, 167, 354, 395, 423, 453, 494
CviKI_1 RGCY 8 cut(s) 26, 153, 167, 354, 395, 423, 453, 494
DdeI CTNAG 1 cut(s) 495
DpnI GATC 1 cut(s) 261
DpnII GATC 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 265
Eco147I AGGCCT 1 cut(s) 453
EcoT14I CCWWGG 1 cut(s) 265
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 3 cut(s) 43, 227, 413
FaiI YATR 6 cut(s) 41, 225, 326, 411, 426, 482
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FatI CATG 3 cut(s) 39, 223, 409
FblI GTMKAC 1 cut(s) 308
Fnu4HI GCNGC 2 cut(s) 6, 69
Fsp4HI GCNGC 2 cut(s) 6, 69
FspI TGCGCA 1 cut(s) 44
GlaI GCGC 1 cut(s) 44
GluI GCNGC 2 cut(s) 6, 69
GsuI CTGGAG 1 cut(s) 381
HaeIII GGCC 1 cut(s) 453
HapII CCGG 1 cut(s) 461
HgaI GACGC 1 cut(s) 84
HhaI GCGC 1 cut(s) 45
Hin1II CATG 3 cut(s) 43, 227, 413
Hin6I GCGC 1 cut(s) 43
HinP1I GCGC 1 cut(s) 43
HincII GTYRAC 2 cut(s) 136, 309
HindII GTYRAC 2 cut(s) 136, 309
HindIII AAGCTT 1 cut(s) 151
HinfI GANTC 1 cut(s) 203
HpaII CCGG 1 cut(s) 461
HphI GGTGA 1 cut(s) 254
Hpy166II GTNNAC 2 cut(s) 136, 309
Hpy188I TCNGA 2 cut(s) 296, 358
Hpy188III TCNNGA 2 cut(s) 398, 461
Hpy8I GTNNAC 2 cut(s) 136, 309
Hpy99I CGWCG 1 cut(s) 313
HpyAV CCTTC 2 cut(s) 460, 469
HpyCH4IV ACGT 2 cut(s) 132, 138
HpyCH4V TGCA 4 cut(s) 14, 68, 81, 418
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 51
HpyF3I CTNAG 1 cut(s) 495
HpySE526I ACGT 2 cut(s) 132, 138
Hsp92II CATG 3 cut(s) 43, 227, 413
HspAI GCGC 1 cut(s) 43
Kpn2I TCCGGA 1 cut(s) 460
Kzo9I GATC 1 cut(s) 259
LmnI GCTCC 1 cut(s) 400
LpnPI CCDG 8 cut(s) 45, 191, 201, 342, 411, 435, 474, 476
Lsp1109I GCAGC 2 cut(s) 17, 80
LweI GCATC 2 cut(s) 23, 90
MaeII ACGT 2 cut(s) 132, 138
MaeIII GTNAC 1 cut(s) 439
MalI GATC 1 cut(s) 261
MboI GATC 1 cut(s) 259
MboII GAAGA 3 cut(s) 102, 105, 148
MluCI AATT 2 cut(s) 59, 125
MluI ACGCGT 1 cut(s) 93
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 2 cut(s) 263, 274
MnlI CCTC 3 cut(s) 213, 290, 397
MroI TCCGGA 1 cut(s) 460
MseI TTAA 1 cut(s) 255
MspI CCGG 1 cut(s) 461
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 2 cut(s) 11, 51
NdeII GATC 1 cut(s) 259
NlaIII CATG 3 cut(s) 43, 227, 413
NmeAIII GCCGAG 1 cut(s) 193
NsbI TGCGCA 1 cut(s) 44
NspI RCATGY 2 cut(s) 227, 413
PaeI GCATGC 1 cut(s) 227
PceI AGGCCT 1 cut(s) 453
PkrI GCNGC 2 cut(s) 7, 70
PleI GAGTC 1 cut(s) 197
PpsI GAGTC 1 cut(s) 197
Psp1406I AACGTT 1 cut(s) 132
SalI GTCGAC 1 cut(s) 307
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 2 cut(s) 6, 69
Sau3AI GATC 1 cut(s) 259
SchI GAGTC 1 cut(s) 197
SetI ASST 7 cut(s) 135, 141, 155, 267, 331, 397, 425
SfaNI GCATC 2 cut(s) 23, 90
SphI GCATGC 1 cut(s) 227
Sse9I AATT 2 cut(s) 59, 125
SseBI AGGCCT 1 cut(s) 453
SsiI CCGC 1 cut(s) 320
SspI AATATT 1 cut(s) 362
StuI AGGCCT 1 cut(s) 453
StyI CCWWGG 1 cut(s) 265
TaiI ACGT 2 cut(s) 135, 141
TaqI TCGA 2 cut(s) 308, 348
TaqII GACCGA 1 cut(s) 74
TasI AATT 2 cut(s) 59, 125
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TseI GCWGC 2 cut(s) 5, 68
TspDTI ATGAA 1 cut(s) 28
XapI RAATTY 2 cut(s) 59, 125
XceI RCATGY 2 cut(s) 227, 413
XmiI GTMKAC 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.