RLG00000004394

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0008854
Physical Location & Seq
Forward (+)
58974175 .. 58976621
2447 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004394

Sequence Viewer

Length: 417 bp
ATGCCAGCAAGCCGATGTGCCACAGAGTATGTAGCTCGTGGAGTCGAGGGGTATGTGCTTGGTTCTGCCCTCCAATCTGATGTACAGTTTGCTCTTGCCCTTCAATACAATGTGGTGCAATTCAAAAGTGATCGTCTCAAACTAGTGCAAGCTGTGAATACTACTGAGGAAGATCACTCCTTGTTGGGTTTTGCTATTGAACAGCCTCTCCTCAATCGGAAACCCAAACACCACAAGCTCTTCGTCATGGGAGAAGGTGAAGAAGACCCAGGCGTCGAAGACGATCAAGAAGACAATGATGGAGACAGGGTGGTAGATGAGGCTGAGGAAGAGCACGACCAAGAGGACCATGGTATAGTTGGACCGGAAATGGGTCAAGTTCTAGAAGACTCGGGTCGAGACGTTGAAGAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

139

Amino Acids

15.31

Weight (kDa)

4.26

Isoelectric Point (pI)

46.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 272
AcyI GRCGYC 1 cut(s) 273
AfaI GTAC 1 cut(s) 84
AfiI CCNNNNNNNGG 1 cut(s) 371
AgsI TTSAA 4 cut(s) 104, 124, 200, 407
AhlI ACTAGT 1 cut(s) 142
AjnI CCWGG 1 cut(s) 268
AloI GAACNNNNNNTCC 2 cut(s) 192, 224
AluBI AGCT 3 cut(s) 35, 152, 238
AluI AGCT 3 cut(s) 35, 152, 238
Alw21I GWGCWC 1 cut(s) 336
Alw26I GTCTC 3 cut(s) 140, 297, 393
Ama87I CYCGRG 1 cut(s) 391
AspS9I GGNCC 2 cut(s) 346, 362
AsuHPI GGTGA 1 cut(s) 269
AvaI CYCGRG 1 cut(s) 391
AvaII GGWCC 2 cut(s) 346, 362
BauI CACGAG 1 cut(s) 36
BbsI GAAGAC 4 cut(s) 270, 285, 297, 393
Bbv12I GWGCWC 1 cut(s) 336
BbvCI CCTCAGC 1 cut(s) 324
BccI CCATC 1 cut(s) 293
BciT130I CCWGG 1 cut(s) 270
BcoDI GTCTC 3 cut(s) 140, 297, 393
BcuI ACTAGT 1 cut(s) 142
BfaI CTAG 2 cut(s) 143, 383
Bme1390I CCNGG 1 cut(s) 270
Bme18I GGWCC 2 cut(s) 346, 362
BmeT110I CYCGRG 1 cut(s) 391
BmgT120I GGNCC 2 cut(s) 346, 362
BmrFI CCNGG 1 cut(s) 270
BoxI GACNNNNGTC 1 cut(s) 393
BpiI GAAGAC 4 cut(s) 270, 285, 297, 393
Bpu10I CCTNAGC 1 cut(s) 324
BsaHI GRCGYC 1 cut(s) 273
BsaJI CCNNGG 2 cut(s) 268, 349
BsaWI WCCGGW 1 cut(s) 364
BsaXI ACNNNNNCTCC 4 cut(s) 192, 222, 294, 324
Bsc4I CCNNNNNNNGG 1 cut(s) 371
BseBI CCWGG 1 cut(s) 270
BseDI CCNNGG 2 cut(s) 268, 349
BseLI CCNNNNNNNGG 1 cut(s) 371
BseMII CTCAG 2 cut(s) 156, 315
BseRI GAGGAG 1 cut(s) 200
BsiHKAI GWGCWC 1 cut(s) 336
BsiHKCI CYCGRG 1 cut(s) 391
BsiSI CCGG 1 cut(s) 365
BslI CCNNNNNNNGG 1 cut(s) 371
BsmAI GTCTC 3 cut(s) 140, 297, 393
BsmBI CGTCTC 2 cut(s) 140, 393
BsoBI CYCGRG 1 cut(s) 391
Bsp1286I GDGCHC 1 cut(s) 336
Bsp1407I TGTACA 1 cut(s) 82
Bsp143I GATC 3 cut(s) 130, 172, 283
Bsp19I CCATGG 1 cut(s) 349
BspCNI CTCAG 2 cut(s) 157, 316
BspQI GCTCTTC 2 cut(s) 245, 324
BsrGI TGTACA 1 cut(s) 82
BssECI CCNNGG 2 cut(s) 268, 349
BssMI GATC 3 cut(s) 130, 172, 283
BssNI GRCGYC 1 cut(s) 273
BssSI CACGAG 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 349
Bst2BI CACGAG 1 cut(s) 36
Bst2UI CCWGG 1 cut(s) 270
Bst4CI ACNGT 1 cut(s) 87
Bst6I CTCTTC 3 cut(s) 245, 324, 402
BstACI GRCGYC 1 cut(s) 273
BstAUI TGTACA 1 cut(s) 82
BstC8I GCNNGC 3 cut(s) 6, 10, 150
BstDEI CTNAG 2 cut(s) 165, 324
BstDSI CCRYGG 1 cut(s) 349
BstKTI GATC 3 cut(s) 133, 175, 286
BstMAI GTCTC 3 cut(s) 140, 297, 393
BstMBI GATC 3 cut(s) 130, 172, 283
BstNI CCWGG 1 cut(s) 270
BstPAI GACNNNNGTC 1 cut(s) 393
BstSCI CCNGG 1 cut(s) 268
BstV2I GAAGAC 4 cut(s) 270, 285, 297, 393
BtgI CCRYGG 1 cut(s) 349
Cac8I GCNNGC 3 cut(s) 6, 10, 150
Cfr13I GGNCC 2 cut(s) 346, 362
CseI GACGC 1 cut(s) 262
Csp6I GTAC 1 cut(s) 83
CviAII CATG 2 cut(s) 247, 350
CviJI RGCY 7 cut(s) 12, 35, 152, 205, 238, 323, 413
CviKI_1 RGCY 7 cut(s) 12, 35, 152, 205, 238, 323, 413
CviQI GTAC 1 cut(s) 83
DdeI CTNAG 2 cut(s) 165, 324
DpnI GATC 3 cut(s) 132, 174, 285
DpnII GATC 3 cut(s) 130, 172, 283
DrdI GACNNNNNNGTC 1 cut(s) 272
DseDI GACNNNNNNGTC 1 cut(s) 272
Eam1104I CTCTTC 3 cut(s) 245, 324, 402
EarI CTCTTC 3 cut(s) 245, 324, 402
Eco130I CCWWGG 1 cut(s) 349
Eco47I GGWCC 2 cut(s) 346, 362
Eco88I CYCGRG 1 cut(s) 391
EcoRII CCWGG 1 cut(s) 268
EcoT14I CCWWGG 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 349
Esp3I CGTCTC 2 cut(s) 140, 393
FaeI CATG 2 cut(s) 250, 353
FaiI YATR 5 cut(s) 30, 54, 248, 351, 356
FatI CATG 2 cut(s) 246, 349
FspBI CTAG 2 cut(s) 143, 383
HapII CCGG 1 cut(s) 365
HgaI GACGC 1 cut(s) 262
Hin1I GRCGYC 1 cut(s) 273
Hin1II CATG 2 cut(s) 250, 353
HinfI GANTC 2 cut(s) 42, 389
HpaII CCGG 1 cut(s) 365
HphI GGTGA 1 cut(s) 269
Hpy188I TCNGA 2 cut(s) 79, 219
Hpy188III TCNNGA 3 cut(s) 287, 383, 398
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 2 cut(s) 110, 248
HpyCH4III ACNGT 1 cut(s) 87
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 2 cut(s) 118, 148
HpyF3I CTNAG 2 cut(s) 165, 324
HpySE526I ACGT 1 cut(s) 402
Hsp92I GRCGYC 1 cut(s) 273
Hsp92II CATG 2 cut(s) 250, 353
Kzo9I GATC 3 cut(s) 130, 172, 283
LguI GCTCTTC 2 cut(s) 245, 324
LpnPI CCDG 5 cut(s) 18, 255, 282, 292, 378
MaeI CTAG 2 cut(s) 143, 383
MaeII ACGT 1 cut(s) 402
MalI GATC 3 cut(s) 132, 174, 285
MboI GATC 3 cut(s) 130, 172, 283
MboII GAAGA 8 cut(s) 182, 232, 272, 275, 290, 302, 341, 398
MhlI GDGCHC 1 cut(s) 336
MluCI AATT 1 cut(s) 119
MlyI GAGTC 2 cut(s) 51, 383
MmeI TCCRAC 1 cut(s) 340
MnlI CCTC 9 cut(s) 40, 80, 160, 216, 221, 313, 319, 337, 403
MspI CCGG 1 cut(s) 365
MspR9I CCNGG 1 cut(s) 270
MvaI CCWGG 1 cut(s) 270
NcoI CCATGG 1 cut(s) 349
NdeII GATC 3 cut(s) 130, 172, 283
NlaIII CATG 2 cut(s) 250, 353
PciSI GCTCTTC 2 cut(s) 245, 324
PleI GAGTC 2 cut(s) 50, 383
PpsI GAGTC 2 cut(s) 50, 383
PshAI GACNNNNGTC 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PspPI GGNCC 2 cut(s) 346, 362
RsaI GTAC 1 cut(s) 84
RsaNI GTAC 1 cut(s) 83
SapI GCTCTTC 2 cut(s) 245, 324
Sau3AI GATC 3 cut(s) 130, 172, 283
Sau96I GGNCC 2 cut(s) 346, 362
SchI GAGTC 2 cut(s) 51, 383
ScrFI CCNGG 1 cut(s) 270
SduI GDGCHC 1 cut(s) 336
SetI ASST 5 cut(s) 37, 154, 240, 259, 405
SinI GGWCC 2 cut(s) 346, 362
SpeI ACTAGT 1 cut(s) 142
Sse9I AATT 1 cut(s) 119
SspMI CTAG 2 cut(s) 143, 383
StyD4I CCNGG 1 cut(s) 268
StyI CCWWGG 1 cut(s) 349
TaaI ACNGT 1 cut(s) 87
TaiI ACGT 1 cut(s) 405
TaqI TCGA 3 cut(s) 45, 276, 397
TasI AATT 1 cut(s) 119
TatI WGTACW 1 cut(s) 82
VpaK11BI GGWCC 2 cut(s) 346, 362
XbaI TCTAGA 1 cut(s) 382
XcmI CCANNNNNNNNNTGG 2 cut(s) 347, 356
XspI CTAG 2 cut(s) 143, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.