RLG00000036133

Belongs to the oxygen-dependent FAD-linked oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0008854
Physical Location & Seq
Forward (+)
76187312 .. 76191456
4145 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036133

Sequence Viewer

Length: 738 bp
ATGAGGTTCACTGGGGCTCTTGCGAAAAGTCTCCTTTTAAGCCAACCTTTGAGTGGGAGATTTCACTTGGACAACGACAAGAGTGCTTGGGTCGAGTCCAGTGCTACCATCGGAGAGATTTTTACTAGAATTGCTGAGAAAAGTAACATCCATGGTTTTTCGGCTGCTGCTTGCCCTAGTGTTGGTGTTGGTGGTCACTTCAGCGGGGGTGGGTATGGCAACTTAATGAGAAAATATTGTATCACAGCAAATAACATTGTGGATGGCAAAATAGTGGATGTAAATGATGCTACTCTTGATAGGCAGTCAATGGGAGAAGACCTATTTTGGGCCATTAGAGGAGTCAGGCCGTCTCCTGCTCCGGCGCTCGACCCAGGCAGTCTCCGGCTCCGGCGCTCCACCCAGGCAGTCTACCTCTCTCCTCCAGACTCGGGCAGCCCACCACGGCGTCGTCACCAGAGGAATCGATCTCGACGACTCGGTCCAGATCAGAGTCGACCTCGAAGACTGGCTGGTCCTAATCCAGATCGGAAGAATGCTGAACCTGAAGAAAGTGAATCGGAGATTAGGGATGTTGACCAAGCTCCTTCGCCTCCTCCAGCCACCACTTCTCCATGGATCACAGCGCCGAATTCGAAGTCAACGACTTCTCCACCAACGATGACGGCGGTTCCACTCCCAAGTCCTCCTCCGCCGCAGCGATTCATCGAACACCTCGAGCTACAGGTCCACCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BBE FAD_binding_4

Protein Analysis

246

Amino Acids

26.68

Weight (kDa)

9.84

Isoelectric Point (pI)

75.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 13 - 96 3.1e-08 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 480, 513
AccI GTMKAC 2 cut(s) 411, 496
AciI CCGC 4 cut(s) 204, 668, 692, 695
AclWI GGATC 1 cut(s) 626
AcsI RAATTY 1 cut(s) 631
AcuI CTGAAG 2 cut(s) 184, 567
AcyI GRCGYC 1 cut(s) 448
AfiI CCNNNNNNNGG 4 cut(s) 53, 182, 328, 431
AgeI ACCGGT 1 cut(s) 732
AjnI CCWGG 2 cut(s) 373, 402
AluBI AGCT 2 cut(s) 584, 721
AluI AGCT 2 cut(s) 584, 721
Alw26I GTCTC 3 cut(s) 35, 357, 386
AlwI GGATC 1 cut(s) 626
Ama87I CYCGRG 2 cut(s) 430, 716
AoxI GGCC 2 cut(s) 330, 347
ApeKI GCWGC 4 cut(s) 164, 167, 435, 697
ApoI RAATTY 1 cut(s) 631
ArsI GACNNNNNNTTYG 2 cut(s) 623, 655
AsiGI ACCGGT 1 cut(s) 732
AspLEI GCGC 3 cut(s) 367, 396, 628
AspS9I GGNCC 4 cut(s) 330, 482, 515, 727
AsuHPI GGTGA 1 cut(s) 446
AsuII TTCGAA 1 cut(s) 635
AvaI CYCGRG 2 cut(s) 430, 716
AvaII GGWCC 3 cut(s) 482, 515, 727
BanII GRGCYC 1 cut(s) 19
BbsI GAAGAC 2 cut(s) 324, 511
BbvI GCAGC 4 cut(s) 151, 154, 447, 709
BccI CCATC 2 cut(s) 116, 257
BceAI ACGGC 3 cut(s) 334, 461, 681
BcgI CGANNNNNNTGC 4 cut(s) 65, 83, 99, 117
BciT130I CCWGG 2 cut(s) 375, 404
BcoDI GTCTC 3 cut(s) 35, 357, 386
BfaI CTAG 2 cut(s) 126, 177
BfmI CTRYAG 1 cut(s) 722
BfoI RGCGCY 3 cut(s) 368, 397, 629
BisI GCNGC 5 cut(s) 165, 168, 436, 695, 698
BlsI GCNGC 5 cut(s) 166, 169, 437, 696, 699
Bme1390I CCNGG 2 cut(s) 375, 404
Bme18I GGWCC 3 cut(s) 482, 515, 727
BmeT110I CYCGRG 2 cut(s) 430, 716
BmgT120I GGNCC 4 cut(s) 330, 482, 515, 727
BmiI GGNNCC 2 cut(s) 389, 672
BmrFI CCNGG 2 cut(s) 375, 404
BmrI ACTGGG 1 cut(s) 21
BmsI GCATC 1 cut(s) 277
BmuI ACTGGG 1 cut(s) 21
BpiI GAAGAC 2 cut(s) 324, 511
BplI GAGNNNNNCTC 2 cut(s) 484, 516
BpmI CTGGAG 2 cut(s) 408, 582
Bpu14I TTCGAA 1 cut(s) 635
Bsa29I ATCGAT 1 cut(s) 466
BsaHI GRCGYC 1 cut(s) 448
BsaJI CCNNGG 5 cut(s) 151, 373, 402, 443, 614
BsaWI WCCGGW 1 cut(s) 732
BsaXI ACNNNNNCTCC 4 cut(s) 595, 625, 634, 664
Bsc4I CCNNNNNNNGG 4 cut(s) 53, 182, 328, 431
Bse118I RCCGGY 1 cut(s) 732
Bse1I ACTGG 3 cut(s) 16, 99, 513
BseBI CCWGG 2 cut(s) 375, 404
BseCI ATCGAT 1 cut(s) 466
BseDI CCNNGG 5 cut(s) 151, 373, 402, 443, 614
BseGI GGATG 4 cut(s) 147, 268, 283, 577
BseLI CCNNNNNNNGG 4 cut(s) 53, 182, 328, 431
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 3 cut(s) 16, 99, 513
BseRI GAGGAG 4 cut(s) 354, 411, 585, 678
BseXI GCAGC 4 cut(s) 151, 154, 447, 709
BshFI GGCC 2 cut(s) 332, 349
BshTI ACCGGT 1 cut(s) 732
BshVI ATCGAT 1 cut(s) 466
BsiHKCI CYCGRG 2 cut(s) 430, 716
BsiSI CCGG 4 cut(s) 362, 385, 391, 733
BslI CCNNNNNNNGG 4 cut(s) 53, 182, 328, 431
BsmAI GTCTC 3 cut(s) 35, 357, 386
BsmBI CGTCTC 1 cut(s) 357
BsmI GAATGC 1 cut(s) 541
BsnI GGCC 2 cut(s) 332, 349
BsoBI CYCGRG 2 cut(s) 430, 716
Bsp119I TTCGAA 1 cut(s) 635
Bsp1286I GDGCHC 1 cut(s) 19
Bsp143I GATC 4 cut(s) 467, 487, 526, 618
Bsp19I CCATGG 2 cut(s) 151, 614
BspACI CCGC 4 cut(s) 204, 668, 692, 695
BspANI GGCC 2 cut(s) 332, 349
BspCNI CTCAG 1 cut(s) 127
BspDI ATCGAT 1 cut(s) 466
BspLI GGNNCC 2 cut(s) 389, 672
BspPI GGATC 1 cut(s) 626
BspT104I TTCGAA 1 cut(s) 635
BsrFI RCCGGY 1 cut(s) 732
BsrI ACTGG 3 cut(s) 16, 99, 513
BssAI RCCGGY 1 cut(s) 732
BssECI CCNNGG 5 cut(s) 151, 373, 402, 443, 614
BssMI GATC 4 cut(s) 467, 487, 526, 618
BssNI GRCGYC 1 cut(s) 448
BssT1I CCWWGG 2 cut(s) 151, 614
Bst2UI CCWGG 2 cut(s) 375, 404
BstACI GRCGYC 1 cut(s) 448
BstBI TTCGAA 1 cut(s) 635
BstC8I GCNNGC 1 cut(s) 172
BstDEI CTNAG 1 cut(s) 135
BstDSI CCRYGG 3 cut(s) 151, 443, 614
BstF5I GGATG 4 cut(s) 147, 268, 283, 577
BstH2I RGCGCY 3 cut(s) 368, 397, 629
BstHHI GCGC 3 cut(s) 367, 396, 628
BstKTI GATC 4 cut(s) 470, 490, 529, 621
BstMAI GTCTC 3 cut(s) 35, 357, 386
BstMBI GATC 4 cut(s) 467, 487, 526, 618
BstNI CCWGG 2 cut(s) 375, 404
BstSCI CCNGG 2 cut(s) 373, 402
BstSFI CTRYAG 1 cut(s) 722
BstV1I GCAGC 4 cut(s) 151, 154, 447, 709
BstV2I GAAGAC 2 cut(s) 324, 511
Bsu15I ATCGAT 1 cut(s) 466
BsuRI GGCC 2 cut(s) 332, 349
BsuTUI ATCGAT 1 cut(s) 466
BtgI CCRYGG 3 cut(s) 151, 443, 614
BtsCI GGATG 4 cut(s) 147, 268, 283, 577
BtsIMutI CAGTG 2 cut(s) 9, 106
Cac8I GCNNGC 1 cut(s) 172
CfoI GCGC 3 cut(s) 367, 396, 628
Cfr10I RCCGGY 1 cut(s) 732
Cfr13I GGNCC 4 cut(s) 330, 482, 515, 727
ClaI ATCGAT 1 cut(s) 466
CseI GACGC 1 cut(s) 437
CspAI ACCGGT 1 cut(s) 732
CviAII CATG 2 cut(s) 152, 615
DdeI CTNAG 1 cut(s) 135
DpnI GATC 4 cut(s) 469, 489, 528, 620
DpnII GATC 4 cut(s) 467, 487, 526, 618
DrdI GACNNNNNNGTC 2 cut(s) 480, 513
DseDI GACNNNNNNGTC 2 cut(s) 480, 513
EciI GGCGGA 1 cut(s) 681
Eco130I CCWWGG 2 cut(s) 151, 614
Eco24I GRGCYC 1 cut(s) 19
Eco47I GGWCC 3 cut(s) 482, 515, 727
Eco57I CTGAAG 2 cut(s) 184, 567
Eco88I CYCGRG 2 cut(s) 430, 716
EcoRI GAATTC 1 cut(s) 631
EcoRII CCWGG 2 cut(s) 373, 402
EcoT14I CCWWGG 2 cut(s) 151, 614
EcoT38I GRGCYC 1 cut(s) 19
ErhI CCWWGG 2 cut(s) 151, 614
Esp3I CGTCTC 1 cut(s) 357
FaeI CATG 2 cut(s) 155, 618
FaiI YATR 3 cut(s) 153, 216, 616
FalI AAGNNNNNCTT 2 cut(s) 31, 63
FatI CATG 2 cut(s) 151, 614
FauI CCCGC 1 cut(s) 197
FblI GTMKAC 2 cut(s) 411, 496
Fnu4HI GCNGC 5 cut(s) 165, 168, 436, 695, 698
FokI GGATG 4 cut(s) 134, 275, 290, 584
FriOI GRGCYC 1 cut(s) 19
Fsp4HI GCNGC 5 cut(s) 165, 168, 436, 695, 698
FspBI CTAG 2 cut(s) 126, 177
GlaI GCGC 3 cut(s) 366, 395, 627
GluI GCNGC 5 cut(s) 165, 168, 436, 695, 698
GsuI CTGGAG 2 cut(s) 408, 582
HaeII RGCGCY 3 cut(s) 368, 397, 629
HaeIII GGCC 2 cut(s) 332, 349
HapII CCGG 4 cut(s) 362, 385, 391, 733
HgaI GACGC 1 cut(s) 437
HhaI GCGC 3 cut(s) 367, 396, 628
Hin1I GRCGYC 1 cut(s) 448
Hin1II CATG 2 cut(s) 155, 618
Hin6I GCGC 3 cut(s) 365, 394, 626
HinP1I GCGC 3 cut(s) 365, 394, 626
HincII GTYRAC 3 cut(s) 497, 577, 642
HindII GTYRAC 3 cut(s) 497, 577, 642
HinfI GANTC 8 cut(s) 95, 342, 428, 463, 477, 493, 557, 702
HpaII CCGG 4 cut(s) 362, 385, 391, 733
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 6 cut(s) 9, 412, 497, 577, 642, 730
Hpy188I TCNGA 4 cut(s) 113, 492, 531, 562
Hpy188III TCNNGA 5 cut(s) 296, 425, 471, 485, 524
Hpy8I GTNNAC 6 cut(s) 9, 412, 497, 577, 642, 730
Hpy99I CGWCG 2 cut(s) 453, 477
HpyAV CCTTC 1 cut(s) 597
HpyF3I CTNAG 1 cut(s) 135
Hsp92I GRCGYC 1 cut(s) 448
Hsp92II CATG 2 cut(s) 155, 618
HspAI GCGC 3 cut(s) 365, 394, 626
Kzo9I GATC 4 cut(s) 467, 487, 526, 618
LmnI GCTCC 4 cut(s) 364, 393, 401, 589
Lsp1109I GCAGC 4 cut(s) 151, 154, 447, 709
LweI GCATC 1 cut(s) 277
MaeI CTAG 2 cut(s) 126, 177
MaeIII GTNAC 3 cut(s) 143, 194, 452
MalI GATC 4 cut(s) 469, 489, 528, 620
MboI GATC 4 cut(s) 467, 487, 526, 618
MboII GAAGA 4 cut(s) 329, 516, 544, 560
MhlI GDGCHC 1 cut(s) 19
MluCI AATT 2 cut(s) 129, 631
MlyI GAGTC 5 cut(s) 104, 351, 422, 471, 502
MseI TTAA 2 cut(s) 38, 224
MspA1I CMGCKG 1 cut(s) 204
MspI CCGG 4 cut(s) 362, 385, 391, 733
MspR9I CCNGG 2 cut(s) 375, 404
Mva1269I GAATGC 1 cut(s) 541
MvaI CCWGG 2 cut(s) 375, 404
NcoI CCATGG 2 cut(s) 151, 614
NdeII GATC 4 cut(s) 467, 487, 526, 618
NlaIII CATG 2 cut(s) 155, 618
NlaIV GGNNCC 2 cut(s) 389, 672
NmuCI GTSAC 2 cut(s) 194, 452
NspV TTCGAA 1 cut(s) 635
PaeR7I CTCGAG 1 cut(s) 716
PcsI WCGNNNNNNNCGW 3 cut(s) 472, 641, 714
PctI GAATGC 1 cut(s) 541
PfeI GAWTC 3 cut(s) 463, 557, 702
PflFI GACNNNGTC 1 cut(s) 480
PinAI ACCGGT 1 cut(s) 732
PkrI GCNGC 5 cut(s) 166, 169, 437, 696, 699
PleI GAGTC 5 cut(s) 103, 350, 422, 471, 501
PpsI GAGTC 5 cut(s) 103, 350, 422, 471, 501
Psp6I CCWGG 2 cut(s) 373, 402
PspGI CCWGG 2 cut(s) 373, 402
PspN4I GGNNCC 2 cut(s) 389, 672
PspPI GGNCC 4 cut(s) 330, 482, 515, 727
PspXI VCTCGAGB 1 cut(s) 716
PsyI GACNNNGTC 1 cut(s) 480
SalI GTCGAC 1 cut(s) 495
SaqAI TTAA 2 cut(s) 38, 224
SatI GCNGC 5 cut(s) 165, 168, 436, 695, 698
Sau3AI GATC 4 cut(s) 467, 487, 526, 618
Sau96I GGNCC 4 cut(s) 330, 482, 515, 727
SchI GAGTC 5 cut(s) 104, 351, 422, 471, 502
ScrFI CCNGG 2 cut(s) 375, 404
SduI GDGCHC 1 cut(s) 19
SfaNI GCATC 1 cut(s) 277
SfcI CTRYAG 1 cut(s) 722
Sfr274I CTCGAG 1 cut(s) 716
SfuI TTCGAA 1 cut(s) 635
SinI GGWCC 3 cut(s) 482, 515, 727
SlaI CTCGAG 1 cut(s) 716
SmlI CTYRAG 1 cut(s) 716
SmoI CTYRAG 1 cut(s) 716
Sse9I AATT 2 cut(s) 129, 631
SsiI CCGC 4 cut(s) 204, 668, 692, 695
SspI AATATT 1 cut(s) 236
SspMI CTAG 2 cut(s) 126, 177
StyD4I CCNGG 2 cut(s) 373, 402
StyI CCWWGG 2 cut(s) 151, 614
TaqI TCGA 9 cut(s) 93, 369, 466, 472, 496, 502, 635, 708, 717
TaqII GACCGA 1 cut(s) 470
TasI AATT 2 cut(s) 129, 631
TauI GCSGC 1 cut(s) 697
TfiI GAWTC 3 cut(s) 463, 557, 702
Tru1I TTAA 2 cut(s) 38, 224
Tru9I TTAA 2 cut(s) 38, 224
TscAI CASTG 2 cut(s) 16, 106
TseFI GTSAC 2 cut(s) 194, 452
TseI GCWGC 4 cut(s) 164, 167, 435, 697
Tsp45I GTSAC 2 cut(s) 194, 452
TspDTI ATGAA 1 cut(s) 694
TspRI CASTG 2 cut(s) 16, 106
Tth111I GACNNNGTC 1 cut(s) 480
VpaK11BI GGWCC 3 cut(s) 482, 515, 727
XapI RAATTY 1 cut(s) 631
XcmI CCANNNNNNNNNTGG 1 cut(s) 50
XhoI CTCGAG 1 cut(s) 716
XmiI GTMKAC 2 cut(s) 411, 496
XspI CTAG 2 cut(s) 126, 177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.