RLG00000021265

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008854
Physical Location & Seq
Reverse (-)
73542847 .. 73544996
2150 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021265

Sequence Viewer

Length: 540 bp
ATGTTGCTGGAAGCTCGGACTAGGATTGCACCCAGATCAAGCCTCACGATTCGTATTTTTCGGATGATGGGTGCGCTAGATATGTCCCGATATCTCTCTCCCTCCCTTATTCCCTTCCTACTGACCTCCGGCTCCGGCGCACTAGTCAAGCCGTCTCCTGCTCCGGCGCTCGACCCAGGCAGTCTCCGGCTCCGGCGCTCCACCCAGGCAGTCTACCTCTCTCCTCCAGACTCGGGCCGCCCACCATGGCGTCGTCACCAGAGGAATCGATCTCGACGACTCGGTCCAGATCGGAGTCGACCTCGACGACTGGCTGGTCCTAATCCAGATTCGAAGAATGCTGAACCTGAAGGAAGTGAATCGGAGATTGGGGATGTTGACCAGGCTCCTTCGCCTCCTCCAGCCACCACTTCTCCAAGGATCACAGCGCCGAATTCGAAGTCGACGACTTCTCCACCAACAATGACGGCGGTTCCACTCCCAAGTCCTCCTCCGCCGCGGCGATTCATCGAACACCTCGAGCTACAGGTCCACCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

180

Amino Acids

19.64

Weight (kDa)

11.62

Isoelectric Point (pI)

93.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 282, 315
AccI GTMKAC 3 cut(s) 213, 298, 443
AccII CGCG 1 cut(s) 499
AciI CCGC 5 cut(s) 238, 470, 494, 497, 499
AclWI GGATC 1 cut(s) 428
AcsI RAATTY 1 cut(s) 433
AcuI CTGAAG 1 cut(s) 369
AcyI GRCGYC 1 cut(s) 250
AfiI CCNNNNNNNGG 1 cut(s) 233
AgeI ACCGGT 1 cut(s) 534
AhlI ACTAGT 1 cut(s) 142
AjnI CCWGG 3 cut(s) 175, 204, 381
AjuI GAANNNNNNNTTGG 2 cut(s) 351, 383
AluBI AGCT 2 cut(s) 14, 523
AluI AGCT 2 cut(s) 14, 523
Alw26I GTCTC 2 cut(s) 159, 188
AlwI GGATC 1 cut(s) 428
Ama87I CYCGRG 2 cut(s) 232, 518
AoxI GGCC 1 cut(s) 235
ApoI RAATTY 1 cut(s) 433
ArsI GACNNNNNNTTYG 2 cut(s) 425, 457
AsiGI ACCGGT 1 cut(s) 534
AspLEI GCGC 5 cut(s) 76, 140, 169, 198, 430
AspS9I GGNCC 4 cut(s) 235, 284, 317, 529
AsuHPI GGTGA 1 cut(s) 248
AsuII TTCGAA 2 cut(s) 332, 437
AvaI CYCGRG 2 cut(s) 232, 518
AvaII GGWCC 3 cut(s) 284, 317, 529
BccI CCATC 1 cut(s) 61
BceAI ACGGC 2 cut(s) 136, 483
BciT130I CCWGG 3 cut(s) 177, 206, 383
BcoDI GTCTC 2 cut(s) 159, 188
BcuI ACTAGT 1 cut(s) 142
BfaI CTAG 3 cut(s) 21, 77, 143
BfmI CTRYAG 1 cut(s) 524
BfoI RGCGCY 3 cut(s) 170, 199, 431
BisI GCNGC 3 cut(s) 238, 497, 500
BlsI GCNGC 3 cut(s) 239, 498, 501
Bme1390I CCNGG 3 cut(s) 177, 206, 383
Bme18I GGWCC 3 cut(s) 284, 317, 529
BmeT110I CYCGRG 2 cut(s) 232, 518
BmgT120I GGNCC 4 cut(s) 235, 284, 317, 529
BmiI GGNNCC 4 cut(s) 133, 191, 387, 474
BmrFI CCNGG 3 cut(s) 177, 206, 383
BplI GAGNNNNNCTC 2 cut(s) 286, 318
BpmI CTGGAG 2 cut(s) 210, 384
Bpu14I TTCGAA 2 cut(s) 332, 437
Bsa29I ATCGAT 1 cut(s) 268
BsaHI GRCGYC 1 cut(s) 250
BsaJI CCNNGG 5 cut(s) 175, 204, 245, 416, 497
BsaWI WCCGGW 1 cut(s) 534
BsaXI ACNNNNNCTCC 4 cut(s) 397, 427, 436, 466
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse118I RCCGGY 1 cut(s) 534
Bse1I ACTGG 1 cut(s) 315
BseBI CCWGG 3 cut(s) 177, 206, 383
BseCI ATCGAT 1 cut(s) 268
BseDI CCNNGG 5 cut(s) 175, 204, 245, 416, 497
BseGI GGATG 2 cut(s) 69, 379
BseLI CCNNNNNNNGG 1 cut(s) 233
BseNI ACTGG 1 cut(s) 315
BseRI GAGGAG 3 cut(s) 213, 387, 480
Bsh1236I CGCG 1 cut(s) 499
BshFI GGCC 1 cut(s) 237
BshTI ACCGGT 1 cut(s) 534
BshVI ATCGAT 1 cut(s) 268
BsiHKCI CYCGRG 2 cut(s) 232, 518
BsiSI CCGG 6 cut(s) 129, 135, 164, 187, 193, 535
BslFI GGGAC 1 cut(s) 70
BslI CCNNNNNNNGG 1 cut(s) 233
BsmAI GTCTC 2 cut(s) 159, 188
BsmBI CGTCTC 1 cut(s) 159
BsmFI GGGAC 1 cut(s) 70
BsmI GAATGC 1 cut(s) 343
BsnI GGCC 1 cut(s) 237
BsoBI CYCGRG 2 cut(s) 232, 518
Bsp119I TTCGAA 2 cut(s) 332, 437
Bsp143I GATC 4 cut(s) 35, 269, 289, 420
Bsp19I CCATGG 1 cut(s) 245
BspACI CCGC 5 cut(s) 238, 470, 494, 497, 499
BspANI GGCC 1 cut(s) 237
BspDI ATCGAT 1 cut(s) 268
BspFNI CGCG 1 cut(s) 499
BspLI GGNNCC 4 cut(s) 133, 191, 387, 474
BspPI GGATC 1 cut(s) 428
BspT104I TTCGAA 2 cut(s) 332, 437
BsrFI RCCGGY 1 cut(s) 534
BsrI ACTGG 1 cut(s) 315
BssAI RCCGGY 1 cut(s) 534
BssECI CCNNGG 5 cut(s) 175, 204, 245, 416, 497
BssMI GATC 4 cut(s) 35, 269, 289, 420
BssNI GRCGYC 1 cut(s) 250
BssT1I CCWWGG 2 cut(s) 245, 416
Bst2UI CCWGG 3 cut(s) 177, 206, 383
BstACI GRCGYC 1 cut(s) 250
BstBI TTCGAA 2 cut(s) 332, 437
BstDSI CCRYGG 2 cut(s) 245, 497
BstF5I GGATG 2 cut(s) 69, 379
BstFNI CGCG 1 cut(s) 499
BstH2I RGCGCY 3 cut(s) 170, 199, 431
BstHHI GCGC 5 cut(s) 76, 140, 169, 198, 430
BstKTI GATC 4 cut(s) 38, 272, 292, 423
BstMAI GTCTC 2 cut(s) 159, 188
BstMBI GATC 4 cut(s) 35, 269, 289, 420
BstNI CCWGG 3 cut(s) 177, 206, 383
BstSCI CCNGG 3 cut(s) 175, 204, 381
BstSFI CTRYAG 1 cut(s) 524
BstUI CGCG 1 cut(s) 499
Bsu15I ATCGAT 1 cut(s) 268
BsuRI GGCC 1 cut(s) 237
BsuTUI ATCGAT 1 cut(s) 268
BtgI CCRYGG 2 cut(s) 245, 497
BtsCI GGATG 2 cut(s) 69, 379
CfoI GCGC 5 cut(s) 76, 140, 169, 198, 430
Cfr10I RCCGGY 1 cut(s) 534
Cfr13I GGNCC 4 cut(s) 235, 284, 317, 529
Cfr42I CCGCGG 1 cut(s) 500
ClaI ATCGAT 1 cut(s) 268
CseI GACGC 1 cut(s) 239
CspAI ACCGGT 1 cut(s) 534
CviAII CATG 1 cut(s) 246
DpnI GATC 4 cut(s) 37, 271, 291, 422
DpnII GATC 4 cut(s) 35, 269, 289, 420
DrdI GACNNNNNNGTC 2 cut(s) 282, 315
DseDI GACNNNNNNGTC 2 cut(s) 282, 315
EciI GGCGGA 1 cut(s) 483
Eco130I CCWWGG 2 cut(s) 245, 416
Eco32I GATATC 1 cut(s) 92
Eco47I GGWCC 3 cut(s) 284, 317, 529
Eco57I CTGAAG 1 cut(s) 369
Eco88I CYCGRG 2 cut(s) 232, 518
EcoRI GAATTC 1 cut(s) 433
EcoRII CCWGG 3 cut(s) 175, 204, 381
EcoRV GATATC 1 cut(s) 92
EcoT14I CCWWGG 2 cut(s) 245, 416
ErhI CCWWGG 2 cut(s) 245, 416
Esp3I CGTCTC 1 cut(s) 159
FaeI CATG 1 cut(s) 249
FaiI YATR 2 cut(s) 83, 247
FaqI GGGAC 1 cut(s) 70
FatI CATG 1 cut(s) 245
FblI GTMKAC 3 cut(s) 213, 298, 443
Fnu4HI GCNGC 3 cut(s) 238, 497, 500
FokI GGATG 2 cut(s) 76, 386
Fsp4HI GCNGC 3 cut(s) 238, 497, 500
FspBI CTAG 3 cut(s) 21, 77, 143
GlaI GCGC 5 cut(s) 75, 139, 168, 197, 429
GluI GCNGC 3 cut(s) 238, 497, 500
GsuI CTGGAG 2 cut(s) 210, 384
HaeII RGCGCY 3 cut(s) 170, 199, 431
HaeIII GGCC 1 cut(s) 237
HapII CCGG 6 cut(s) 129, 135, 164, 187, 193, 535
HgaI GACGC 1 cut(s) 239
HhaI GCGC 5 cut(s) 76, 140, 169, 198, 430
Hin1I GRCGYC 1 cut(s) 250
Hin1II CATG 1 cut(s) 249
Hin6I GCGC 5 cut(s) 74, 138, 167, 196, 428
HinP1I GCGC 5 cut(s) 74, 138, 167, 196, 428
HincII GTYRAC 3 cut(s) 299, 379, 444
HindII GTYRAC 3 cut(s) 299, 379, 444
HinfI GANTC 8 cut(s) 49, 230, 265, 279, 295, 329, 359, 504
HpaII CCGG 6 cut(s) 129, 135, 164, 187, 193, 535
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 5 cut(s) 214, 299, 379, 444, 532
Hpy188I TCNGA 4 cut(s) 18, 63, 294, 364
Hpy188III TCNNGA 6 cut(s) 46, 87, 227, 273, 287, 326
Hpy8I GTNNAC 5 cut(s) 214, 299, 379, 444, 532
Hpy99I CGWCG 4 cut(s) 255, 279, 309, 448
HpyAV CCTTC 3 cut(s) 124, 344, 399
HpyCH4V TGCA 1 cut(s) 29
Hsp92I GRCGYC 1 cut(s) 250
Hsp92II CATG 1 cut(s) 249
HspAI GCGC 5 cut(s) 74, 138, 167, 196, 428
KspI CCGCGG 1 cut(s) 500
Kzo9I GATC 4 cut(s) 35, 269, 289, 420
LmnI GCTCC 5 cut(s) 137, 166, 195, 203, 391
MaeI CTAG 3 cut(s) 21, 77, 143
MaeIII GTNAC 1 cut(s) 254
MalI GATC 4 cut(s) 37, 271, 291, 422
MboI GATC 4 cut(s) 35, 269, 289, 420
MboII GAAGA 1 cut(s) 346
MluCI AATT 1 cut(s) 433
MlyI GAGTC 3 cut(s) 224, 273, 304
MspA1I CMGCKG 1 cut(s) 499
MspI CCGG 6 cut(s) 129, 135, 164, 187, 193, 535
MspR9I CCNGG 3 cut(s) 177, 206, 383
Mva1269I GAATGC 1 cut(s) 343
MvaI CCWGG 3 cut(s) 177, 206, 383
MvnI CGCG 1 cut(s) 499
NcoI CCATGG 1 cut(s) 245
NdeII GATC 4 cut(s) 35, 269, 289, 420
NlaIII CATG 1 cut(s) 249
NlaIV GGNNCC 4 cut(s) 133, 191, 387, 474
NmuCI GTSAC 1 cut(s) 254
NspV TTCGAA 2 cut(s) 332, 437
PaeR7I CTCGAG 1 cut(s) 518
PcsI WCGNNNNNNNCGW 4 cut(s) 274, 304, 443, 516
PctI GAATGC 1 cut(s) 343
PfeI GAWTC 5 cut(s) 49, 265, 329, 359, 504
PflFI GACNNNGTC 1 cut(s) 282
PinAI ACCGGT 1 cut(s) 534
PkrI GCNGC 3 cut(s) 239, 498, 501
PleI GAGTC 3 cut(s) 224, 273, 303
PpsI GAGTC 3 cut(s) 224, 273, 303
Psp6I CCWGG 3 cut(s) 175, 204, 381
PspGI CCWGG 3 cut(s) 175, 204, 381
PspN4I GGNNCC 4 cut(s) 133, 191, 387, 474
PspPI GGNCC 4 cut(s) 235, 284, 317, 529
PspXI VCTCGAGB 1 cut(s) 518
PsyI GACNNNGTC 1 cut(s) 282
SacII CCGCGG 1 cut(s) 500
SalI GTCGAC 2 cut(s) 297, 442
SatI GCNGC 3 cut(s) 238, 497, 500
Sau3AI GATC 4 cut(s) 35, 269, 289, 420
Sau96I GGNCC 4 cut(s) 235, 284, 317, 529
SchI GAGTC 3 cut(s) 224, 273, 304
ScrFI CCNGG 3 cut(s) 177, 206, 383
SetI ASST 8 cut(s) 16, 128, 219, 304, 349, 519, 525, 531
SfcI CTRYAG 1 cut(s) 524
Sfr274I CTCGAG 1 cut(s) 518
Sfr303I CCGCGG 1 cut(s) 500
SfuI TTCGAA 2 cut(s) 332, 437
SgrBI CCGCGG 1 cut(s) 500
SinI GGWCC 3 cut(s) 284, 317, 529
SlaI CTCGAG 1 cut(s) 518
SmlI CTYRAG 1 cut(s) 518
SmoI CTYRAG 1 cut(s) 518
SpeI ACTAGT 1 cut(s) 142
Sse9I AATT 1 cut(s) 433
SsiI CCGC 5 cut(s) 238, 470, 494, 497, 499
SspMI CTAG 3 cut(s) 21, 77, 143
StyD4I CCNGG 3 cut(s) 175, 204, 381
StyI CCWWGG 2 cut(s) 245, 416
TaqII GACCGA 1 cut(s) 272
TasI AATT 1 cut(s) 433
TauI GCSGC 3 cut(s) 240, 499, 502
TfiI GAWTC 5 cut(s) 49, 265, 329, 359, 504
TseFI GTSAC 1 cut(s) 254
Tsp45I GTSAC 1 cut(s) 254
TspDTI ATGAA 1 cut(s) 496
Tth111I GACNNNGTC 1 cut(s) 282
VpaK11BI GGWCC 3 cut(s) 284, 317, 529
XapI RAATTY 1 cut(s) 433
XhoI CTCGAG 1 cut(s) 518
XmiI GTMKAC 3 cut(s) 213, 298, 443
XspI CTAG 3 cut(s) 21, 77, 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.