FvH4_4g24120

Ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002610
Physical Location & Seq
Forward (+)
26398360 .. 26401883
3524 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g24120.t1

Sequence Viewer

Length: 2571 bp
ATGACGGTGTTCGCCGGAAAAGCGCACGTGTTTCCTATCGACTACGAAGCAGAGGTGTCACAGAGGTTGGTCGACGCTGCCCACGAAAATGATCCGAACTCGGTGTATGAGTGTCTGGACGATCCTTTTGTCGACGTGAACTACGTCGGAACAGTGTGCTTGAAGAGTAAAAAGACAGAGATTGTGTTGAAAGGTGAGTCGGCTCACGAGGTTGGTGTCGAGTACGAGGAGTTCAAGACTCAGGTAACCGCCTTGTTCCTCGGAGCTCATTCCGGCAATTTGGCGGTCGTCAGAAAACTACTGAGTTATGGAGCCAATGTGAATCTGAAACTTTTTCGAGGCTACGCAACAACAGCAGCAGTGAGGGAAGGACATACAGAGATACTGGAGGTTCTTCTCAATGCCGGAGCATCTCAGAAGGCCTGCGAGGAGGCCTTGTTGGAGGCAAGCTACTTGGGGCGCGCTAGGCCGACTGAGATGCTCATGGCATCTGAGTTTATTCGTCCTCAAGCCGCTGTTCATGCTCTTGTTGCTGCTTCCTGCAGAGGGTTTGTTGATGTTATAGATACACTCATCAAGTGTGGGGTAAATATCGACGCAACTGACCGGACATTGCTTCAATCTTCGAAGCCATCACTCTACACCAACGCTGACTGTAATGCTCTTGTTGCTGCCATTGTTAGCAGGCAGATTTCGGTTGTTAGACTACTGTTGCAGGCTGGAGCCAGAACAGATACCAAAGTGAGTCTTGGAGGATGGTCCTGGGATGTAAGTACAGGAGAAGAGTTTCGGGTGGGTGCAGGACTAGCCGAGGCTTACAGTGTGACCTGGTGTGCTGTGGAGTACTTAGAAGCCAGCGGTGCTATCTTGCGAATGCTGCTACAACATGTCTCCCCTAATATTGCTCACTTTGGAAGGACTCTCATCCACCATGCCATCTTGTGCAATAATGAAAGAGCAGTAGATGTGCTGTTGAGCTGTGGCGCAGATGTAGAGGTTCCGATTAAGACAACTACTTCAGAAACTGAGTATCCCATCCACATGGCTTCGCGGTTTGGGTTAAGTAAGATTCTCCAACAACTGATTGATGGTGGCTGCAATGTCAACTCTCGAACAGACTCTAGAGAAACTCCTCTCATGATCTGTGCTAGACATAAACACCAGGAGTGTTTCAGAATTCTTGCTTCCAATGGTGCTGATTTTGGCCTTGTCAATTCTCGTGGTCAAAGTGCAAGTGTGATTGCAGAGTCAGCTAAGTGGAGTCTTGGCTTTAAAAGAGTAATTCTAGACTTGATTGGAGCTGGGAAGGTTGTTCGATCGAGCAATATGACAATCTTTTCTCCTCTATTGTTTGTGACTCAAGAAAATGATGTTGAGGCTTTGAAACGTCTGATCAGTTCTGGTTGTGATATCAACTCTCAAACAGAATCTGGAGAAACTGCACTAATGATCTGCGCAAGACATAAACACCAGGAATGCTTCAAAGTTCTTGCTTCAAATGGAGCTGATTTTGGCCTTGTCAACTCCAGTGGTCAAAGTGCAAGCTCCATTGCAGAGTCAGCTAACTGGACTCCTGTCTTCAAACAAGATATCCTAGATGTGATTGGCGAGGGGAAGGTTATCCGGTCAAGCAACACATCAATATGTTCTCCTCTAATGCTTGTGACTCAAGAAAACAATGTTGAGGCTTTGAAACAACTGATTGAGAGGACAGACATTGATGTTGATGAGCAAGACGAAAATGGATACTCGGCTGCCATGATAGCTGCAGCAGATGGTAACTTGGAAGCTTTCAAATTGCTTATCTGTGCCAGAGCTGACATGAATCTGCAGAACAAACATGGTCAGACCCCATTGGACCTACTAGATAAAAATCAACATGGTGAAGAATTTGAAAAATTATTACTGAATCATGCACTTGAAAAGGGCCCCGATAGTAGTTCTCTCGAGTTCTATGCGCTACATCTTGCAGCGCAACATGGAGACTTGGATTTGGTTCATGCATTGATAAGTCACGGCTATGATGTTAATGCTTTTGATGCTGAGGGATACACTTCATTGATGTTAGCTGCAAGAGGAGGCCATGGTAAGCTGTGTGAGCTTCTAATTTCTTTTGGGGCTAGATGTGATACTGTGAATGCAAGACATGAGACTGCGCTGTCTCTTGCGAGGAAAACTGGATCGAAAAATGATGCAGAGAAGGCCATATTGAATGAGTTTGCACTAAAACTTGTGCTGGGTGGGAGTAATGTCAAGAAGCACACAAAATGGGGTAAAGGAGCTCCCCATAATAAAGTGCTGAGAATGGTTGGGGCTGCAGGGCTTTTAAGATGGGGGAAGTCAAGTAAGAGAAATGTGATCTGCAAGAAGGCGGAGGTCGGGCCTAGTGATGCATTCAGGTGGAACCGCAGGAGGAAGTATGACTGTGATGAACCAGGGATGTTCCATGTGGTGACTACAAAGAACAAGGAGCTGCATTTTGTGTGTGAAGGTGGGATTGAAATGGCGGATTTGTGGGTGAGAGGGATCAAGCTTGTGACAATGAAAGCTATCTTTGGCAAGAATGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4

Protein Analysis

857

Amino Acids

93.22

Weight (kDa)

6.18

Isoelectric Point (pI)

32.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 340 - 402 1.8e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 452 - 514 1.1e-09 Ankyrin repeats (3 copies)
Ank_4 PF13637 455 - 497 2.8e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 552 - 638 6.6e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 567 - 621 2.8e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 654 - 730 4.6e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 654 - 703 5.6e-10 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2158
Acc16I TGCGCA 1 cut(s) 1456
AccI GTMKAC 2 cut(s) 72, 132
AccII CGCG 2 cut(s) 462, 1051
AciI CCGC 8 cut(s) 249, 284, 513, 858, 1051, 2372, 2407, 2507
AclWI GGATC 4 cut(s) 86, 116, 2188, 2534
AcsI RAATTY 2 cut(s) 1176, 1889
AcuI CTGAAG 1 cut(s) 1002
AcvI CACGTG 1 cut(s) 28
AfaI GTAC 3 cut(s) 224, 775, 845
AfiI CCNNNNNNNGG 1 cut(s) 546
AflIII ACRYGT 2 cut(s) 27, 886
AjiI CACGTC 1 cut(s) 136
AjnI CCWGG 5 cut(s) 761, 827, 1161, 1470, 2434
Alw21I GWGCWC 2 cut(s) 268, 2284
Alw26I GTCTC 4 cut(s) 895, 1977, 2144, 2166
AlwI GGATC 4 cut(s) 86, 116, 2188, 2534
AlwNI CAGNNNCTG 2 cut(s) 1025, 1430
Ama87I CYCGRG 1 cut(s) 1946
AoxI GGCC 9 cut(s) 420, 432, 467, 1204, 1513, 1927, 2080, 2202, 2381
ApaI GGGCCC 1 cut(s) 1931
ApoI RAATTY 2 cut(s) 1176, 1889
ArsI GACNNNNNNTTYG 2 cut(s) 110, 142
Asp700I GAANNNNTTC 1 cut(s) 786
AspLEI GCGC 8 cut(s) 25, 462, 464, 986, 1457, 1960, 1975, 2158
AspS9I GGNCC 5 cut(s) 759, 1858, 1927, 1928, 2381
AsuHPI GGTGA 4 cut(s) 206, 1895, 2464, 2530
AsuII TTCGAA 1 cut(s) 626
AvaI CYCGRG 1 cut(s) 1946
AvaII GGWCC 2 cut(s) 759, 1858
BaeGI GKGCMC 1 cut(s) 1931
BaeI ACNNNNGTAYC 2 cut(s) 726, 759
BanII GRGCYC 3 cut(s) 268, 1931, 2284
BauI CACGAG 2 cut(s) 206, 1218
BbrPI CACGTG 1 cut(s) 28
BbsI GAAGAC 1 cut(s) 1570
Bbv12I GWGCWC 2 cut(s) 268, 2284
BbvCI CCTCAGC 1 cut(s) 2043
BccI CCATC 7 cut(s) 640, 750, 944, 1043, 1082, 1769, 2325
BceAI ACGGC 1 cut(s) 2032
BcgI CGANNNNNNTGC 4 cut(s) 460, 494, 1937, 1971
BciT130I CCWGG 5 cut(s) 763, 829, 1163, 1472, 2436
BciVI GTATCC 3 cut(s) 1041, 1739, 2042
BclI TGATCA 1 cut(s) 1392
BcoDI GTCTC 4 cut(s) 895, 1977, 2144, 2166
BfaI CTAG 9 cut(s) 465, 806, 1122, 1149, 1286, 1595, 1865, 2121, 2385
BfmI CTRYAG 4 cut(s) 541, 1767, 1829, 2316
BfuI GTATCC 3 cut(s) 1041, 1739, 2042
BmcAI AGTACT 1 cut(s) 845
Bme1390I CCNGG 5 cut(s) 763, 829, 1163, 1472, 2436
Bme18I GGWCC 2 cut(s) 759, 1858
BmeT110I CYCGRG 1 cut(s) 1946
BmgBI CACGTC 1 cut(s) 136
BmgT120I GGNCC 5 cut(s) 759, 1858, 1927, 1928, 2381
BmiI GGNNCC 6 cut(s) 313, 724, 999, 1929, 1930, 2405
BmrFI CCNGG 5 cut(s) 763, 829, 1163, 1472, 2436
BmsI GCATC 6 cut(s) 419, 468, 497, 2029, 2182, 2380
BoxI GACNNNNGTC 1 cut(s) 1574
BpiI GAAGAC 1 cut(s) 1570
BpmI CTGGAG 4 cut(s) 407, 741, 1452, 1510
Bpu10I CCTNAGC 1 cut(s) 2043
Bpu14I TTCGAA 1 cut(s) 626
BpuEI CTTGAG 3 cut(s) 492, 1344, 1653
BsaAI YACGTR 1 cut(s) 28
BsaBI GATNNNNATC 1 cut(s) 1872
BsaJI CCNNGG 5 cut(s) 259, 762, 810, 2083, 2435
BsaWI WCCGGW 2 cut(s) 606, 1623
BsaXI ACNNNNNCTCC 4 cut(s) 303, 333, 422, 452
Bsc4I CCNNNNNNNGG 1 cut(s) 546
Bse1I ACTGG 4 cut(s) 390, 1527, 1571, 2182
Bse3DI GCAATG 3 cut(s) 611, 1105, 1548
Bse8I GATNNNNATC 1 cut(s) 1872
BseBI CCWGG 5 cut(s) 763, 829, 1163, 1472, 2436
BseDI CCNNGG 5 cut(s) 259, 762, 810, 2083, 2435
BseGI GGATG 5 cut(s) 761, 772, 924, 1035, 2445
BseJI GATNNNNATC 1 cut(s) 1872
BseLI CCNNNNNNNGG 1 cut(s) 546
BseMI GCAATG 3 cut(s) 611, 1105, 1548
BseMII CTCAG 8 cut(s) 254, 293, 428, 465, 483, 1017, 2034, 2291
BseNI ACTGG 4 cut(s) 390, 1527, 1571, 2182
BsePI GCGCGC 1 cut(s) 460
BseRI GAGGAG 6 cut(s) 242, 443, 1122, 1332, 1641, 2091
BseSI GKGCMC 1 cut(s) 1931
BseYI CCCAGC 2 cut(s) 1301, 2236
BsgI GTGCAG 2 cut(s) 819, 1425
Bsh1236I CGCG 2 cut(s) 462, 1051
Bsh1285I CGRYCG 2 cut(s) 288, 1319
BshFI GGCC 9 cut(s) 422, 434, 469, 1206, 1515, 1929, 2082, 2204, 2383
BsiEI CGRYCG 2 cut(s) 288, 1319
BsiHKAI GWGCWC 2 cut(s) 268, 2284
BsiHKCI CYCGRG 1 cut(s) 1946
BsiSI CCGG 5 cut(s) 15, 273, 405, 607, 1624
BslI CCNNNNNNNGG 1 cut(s) 546
BsmAI GTCTC 4 cut(s) 895, 1977, 2144, 2166
BsmI GAATGC 4 cut(s) 879, 1481, 2143, 2393
BsnI GGCC 9 cut(s) 422, 434, 469, 1206, 1515, 1929, 2082, 2204, 2383
BsoBI CYCGRG 1 cut(s) 1946
Bsp119I TTCGAA 1 cut(s) 626
Bsp120I GGGCCC 1 cut(s) 1927
Bsp1286I GDGCHC 3 cut(s) 268, 1931, 2284
Bsp143I GATC 9 cut(s) 91, 121, 1140, 1316, 1392, 1449, 2180, 2358, 2526
Bsp19I CCATGG 1 cut(s) 2083
BspACI CCGC 8 cut(s) 249, 284, 513, 858, 1051, 2372, 2407, 2507
BspANI GGCC 9 cut(s) 422, 434, 469, 1206, 1515, 1929, 2082, 2204, 2383
BspCNI CTCAG 8 cut(s) 253, 294, 427, 466, 484, 1018, 2035, 2292
BspFNI CGCG 2 cut(s) 462, 1051
BspHI TCATGA 1 cut(s) 1137
BspLI GGNNCC 6 cut(s) 313, 724, 999, 1929, 1930, 2405
BspMAI CTGCAG 4 cut(s) 545, 1771, 1833, 2320
BspPI GGATC 4 cut(s) 86, 116, 2188, 2534
BspT104I TTCGAA 1 cut(s) 626
BsrDI GCAATG 3 cut(s) 611, 1105, 1548
BsrI ACTGG 4 cut(s) 390, 1527, 1571, 2182
BssECI CCNNGG 5 cut(s) 259, 762, 810, 2083, 2435
BssHII GCGCGC 1 cut(s) 460
BssMI GATC 9 cut(s) 91, 121, 1140, 1316, 1392, 1449, 2180, 2358, 2526
BssSI CACGAG 2 cut(s) 206, 1218
BssT1I CCWWGG 1 cut(s) 2083
Bst2BI CACGAG 2 cut(s) 206, 1218
Bst2UI CCWGG 5 cut(s) 763, 829, 1163, 1472, 2436
Bst4CI ACNGT 7 cut(s) 7, 154, 656, 711, 821, 2134, 2426
Bst6I CTCTTC 2 cut(s) 158, 777
BstBAI YACGTR 1 cut(s) 28
BstBI TTCGAA 1 cut(s) 626
BstC8I GCNNGC 7 cut(s) 424, 448, 462, 686, 717, 856, 1543
BstDSI CCRYGG 1 cut(s) 2083
BstEII GGTNACC 1 cut(s) 244
BstF5I GGATG 5 cut(s) 761, 772, 924, 1035, 2445
BstFNI CGCG 2 cut(s) 462, 1051
BstHHI GCGC 8 cut(s) 25, 462, 464, 986, 1457, 1960, 1975, 2158
BstKTI GATC 9 cut(s) 94, 124, 1143, 1319, 1395, 1452, 2183, 2361, 2529
BstMAI GTCTC 4 cut(s) 895, 1977, 2144, 2166
BstMBI GATC 9 cut(s) 91, 121, 1140, 1316, 1392, 1449, 2180, 2358, 2526
BstMCI CGRYCG 2 cut(s) 288, 1319
BstNI CCWGG 5 cut(s) 763, 829, 1163, 1472, 2436
BstNSI RCATGY 1 cut(s) 890
BstPAI GACNNNNGTC 1 cut(s) 1574
BstPI GGTNACC 1 cut(s) 244
BstSCI CCNGG 5 cut(s) 761, 827, 1161, 1470, 2434
BstSFI CTRYAG 4 cut(s) 541, 1767, 1829, 2316
BstSLI GKGCMC 1 cut(s) 1931
BstUI CGCG 2 cut(s) 462, 1051
BstV2I GAAGAC 1 cut(s) 1570
BstXI CCANNNNNNTGG 1 cut(s) 1042
BsuI GTATCC 3 cut(s) 1041, 1739, 2042
BsuRI GGCC 9 cut(s) 422, 434, 469, 1206, 1515, 1929, 2082, 2204, 2383
BtgI CCRYGG 1 cut(s) 2083
BtrI CACGTC 1 cut(s) 136
BtsCI GGATG 5 cut(s) 761, 772, 924, 1035, 2445
BtsI GCAGTG 1 cut(s) 366
BtsIMutI CAGTG 4 cut(s) 159, 366, 826, 1534
Cac8I GCNNGC 7 cut(s) 424, 448, 462, 686, 717, 856, 1543
CaiI CAGNNNCTG 2 cut(s) 1025, 1430
CciI TCATGA 1 cut(s) 1137
CfoI GCGC 8 cut(s) 25, 462, 464, 986, 1457, 1960, 1975, 2158
Cfr13I GGNCC 5 cut(s) 759, 1858, 1927, 1928, 2381
CseI GACGC 2 cut(s) 83, 605
CsiI ACCWGGT 1 cut(s) 827
Csp6I GTAC 3 cut(s) 223, 774, 844
CspCI CAANNNNNGTGG 4 cut(s) 1201, 1236, 1510, 1545
CviQI GTAC 3 cut(s) 223, 774, 844
DpnI GATC 9 cut(s) 93, 123, 1142, 1318, 1394, 1451, 2182, 2360, 2528
DpnII GATC 9 cut(s) 91, 121, 1140, 1316, 1392, 1449, 2180, 2358, 2526
DraI TTTAAA 1 cut(s) 1273
DrdI GACNNNNNNGTC 1 cut(s) 2158
DseDI GACNNNNNNGTC 1 cut(s) 2158
Eam1104I CTCTTC 2 cut(s) 158, 777
EarI CTCTTC 2 cut(s) 158, 777
EciI GGCGGA 2 cut(s) 2387, 2522
Ecl136II GAGCTC 2 cut(s) 266, 2282
Eco130I CCWWGG 1 cut(s) 2083
Eco147I AGGCCT 2 cut(s) 422, 434
Eco24I GRGCYC 3 cut(s) 268, 1931, 2284
Eco32I GATATC 2 cut(s) 1411, 1591
Eco47I GGWCC 2 cut(s) 759, 1858
Eco53kI GAGCTC 2 cut(s) 266, 2282
Eco57I CTGAAG 1 cut(s) 1002
Eco72I CACGTG 1 cut(s) 28
Eco88I CYCGRG 1 cut(s) 1946
Eco91I GGTNACC 1 cut(s) 244
EcoICRI GAGCTC 2 cut(s) 266, 2282
EcoO109I RGGNCCY 2 cut(s) 1927, 1928
EcoO65I GGTNACC 1 cut(s) 244
EcoRI GAATTC 1 cut(s) 1176
EcoRII CCWGG 5 cut(s) 761, 827, 1161, 1470, 2434
EcoRV GATATC 2 cut(s) 1411, 1591
EcoT14I CCWWGG 1 cut(s) 2083
EcoT22I ATGCAT 2 cut(s) 2005, 2395
EcoT38I GRGCYC 3 cut(s) 268, 1931, 2284
ErhI CCWWGG 1 cut(s) 2083
FalI AAGNNNNNCTT 2 cut(s) 732, 764
FbaI TGATCA 1 cut(s) 1392
FblI GTMKAC 2 cut(s) 72, 132
FokI GGATG 5 cut(s) 768, 779, 911, 1022, 2452
FriOI GRGCYC 3 cut(s) 268, 1931, 2284
FspBI CTAG 9 cut(s) 465, 806, 1122, 1149, 1286, 1595, 1865, 2121, 2385
FspI TGCGCA 1 cut(s) 1456
GlaI GCGC 8 cut(s) 24, 461, 463, 985, 1456, 1959, 1974, 2157
GsaI CCCAGC 2 cut(s) 1305, 2240
GsuI CTGGAG 4 cut(s) 407, 741, 1452, 1510
HaeIII GGCC 9 cut(s) 422, 434, 469, 1206, 1515, 1929, 2082, 2204, 2383
HapII CCGG 5 cut(s) 15, 273, 405, 607, 1624
HgaI GACGC 2 cut(s) 83, 605
HhaI GCGC 8 cut(s) 25, 462, 464, 986, 1457, 1960, 1975, 2158
Hin6I GCGC 8 cut(s) 23, 460, 462, 984, 1455, 1958, 1973, 2156
HinP1I GCGC 8 cut(s) 23, 460, 462, 984, 1455, 1958, 1973, 2156
HincII GTYRAC 4 cut(s) 73, 133, 1105, 1522
HindII GTYRAC 4 cut(s) 73, 133, 1105, 1522
HindIII AAGCTT 2 cut(s) 1788, 2531
HpaII CCGG 5 cut(s) 15, 273, 405, 607, 1624
HphI GGTGA 4 cut(s) 206, 1895, 2464, 2530
Hpy166II GTNNAC 5 cut(s) 73, 133, 139, 1105, 1522
Hpy8I GTNNAC 5 cut(s) 73, 133, 139, 1105, 1522
Hpy99I CGWCG 4 cut(s) 77, 137, 149, 599
HpyAV CCTTC 8 cut(s) 362, 412, 909, 1300, 1609, 2194, 2362, 2483
HpyCH4III ACNGT 7 cut(s) 7, 154, 656, 711, 821, 2134, 2426
HpyCH4IV ACGT 4 cut(s) 27, 135, 144, 1387
HpySE526I ACGT 4 cut(s) 27, 135, 144, 1387
HspAI GCGC 8 cut(s) 23, 460, 462, 984, 1455, 1958, 1973, 2156
Ksp22I TGATCA 1 cut(s) 1392
Kzo9I GATC 9 cut(s) 91, 121, 1140, 1316, 1392, 1449, 2180, 2358, 2526
LweI GCATC 6 cut(s) 419, 468, 497, 2029, 2182, 2380
MabI ACCWGGT 1 cut(s) 827
MaeI CTAG 9 cut(s) 465, 806, 1122, 1149, 1286, 1595, 1865, 2121, 2385
MaeII ACGT 4 cut(s) 27, 135, 144, 1387
MaeIII GTNAC 9 cut(s) 57, 244, 823, 1354, 1663, 1778, 2012, 2452, 2536
MalI GATC 9 cut(s) 93, 123, 1142, 1318, 1394, 1451, 2182, 2360, 2528
MboI GATC 9 cut(s) 91, 121, 1140, 1316, 1392, 1449, 2180, 2358, 2526
MboII GAAGA 6 cut(s) 175, 386, 615, 794, 1570, 1898
MhlI GDGCHC 3 cut(s) 268, 1931, 2284
MluCI AATT 8 cut(s) 277, 1176, 1213, 1281, 1796, 1889, 1898, 2106
MmeI TCCRAC 3 cut(s) 127, 420, 1099
Mph1103I ATGCAT 2 cut(s) 2005, 2395
MroXI GAANNNNTTC 1 cut(s) 786
MseI TTAA 5 cut(s) 1005, 1061, 1272, 2028, 2327
MslI CAYNNNNRTG 5 cut(s) 87, 1040, 1642, 2019, 2398
MspA1I CMGCKG 2 cut(s) 515, 858
MspI CCGG 5 cut(s) 15, 273, 405, 607, 1624
MspR9I CCNGG 5 cut(s) 763, 829, 1163, 1472, 2436
Mva1269I GAATGC 4 cut(s) 879, 1481, 2143, 2393
MvaI CCWGG 5 cut(s) 763, 829, 1163, 1472, 2436
MvnI CGCG 2 cut(s) 462, 1051
NcoI CCATGG 1 cut(s) 2083
NdeII GATC 9 cut(s) 91, 121, 1140, 1316, 1392, 1449, 2180, 2358, 2526
NlaIV GGNNCC 6 cut(s) 313, 724, 999, 1929, 1930, 2405
NmeAIII GCCGAG 2 cut(s) 835, 1730
NmuCI GTSAC 7 cut(s) 57, 823, 1354, 1663, 2012, 2452, 2536
NsbI TGCGCA 1 cut(s) 1456
NsiI ATGCAT 2 cut(s) 2005, 2395
NspI RCATGY 1 cut(s) 890
NspV TTCGAA 1 cut(s) 626
PaeR7I CTCGAG 1 cut(s) 1946
PagI TCATGA 1 cut(s) 1137
PauI GCGCGC 1 cut(s) 460
PceI AGGCCT 2 cut(s) 422, 434
PciI ACATGT 1 cut(s) 886
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 141
PctI GAATGC 4 cut(s) 879, 1481, 2143, 2393
PdmI GAANNNNTTC 1 cut(s) 786
PfeI GAWTC 5 cut(s) 322, 1069, 1427, 1825, 1909
Ple19I CGATCG 1 cut(s) 1319
PmaCI CACGTG 1 cut(s) 28
PmlI CACGTG 1 cut(s) 28
Ppu21I YACGTR 1 cut(s) 28
PscI ACATGT 1 cut(s) 886
PshAI GACNNNNGTC 1 cut(s) 1574
Psp124BI GAGCTC 2 cut(s) 268, 2284
Psp6I CCWGG 5 cut(s) 761, 827, 1161, 1470, 2434
PspCI CACGTG 1 cut(s) 28
PspEI GGTNACC 1 cut(s) 244
PspFI CCCAGC 2 cut(s) 1301, 2236
PspGI CCWGG 5 cut(s) 761, 827, 1161, 1470, 2434
PspN4I GGNNCC 6 cut(s) 313, 724, 999, 1929, 1930, 2405
PspOMI GGGCCC 1 cut(s) 1927
PspPI GGNCC 5 cut(s) 759, 1858, 1927, 1928, 2381
PsrI GAACNNNNNNTAC 2 cut(s) 215, 247
PstI CTGCAG 4 cut(s) 545, 1771, 1833, 2320
PstNI CAGNNNCTG 2 cut(s) 1025, 1430
PteI GCGCGC 1 cut(s) 460
PvuI CGATCG 1 cut(s) 1319
RsaI GTAC 3 cut(s) 224, 775, 845
RsaNI GTAC 3 cut(s) 223, 774, 844
RseI CAYNNNNRTG 5 cut(s) 87, 1040, 1642, 2019, 2398
SacI GAGCTC 2 cut(s) 268, 2284
SalI GTCGAC 2 cut(s) 71, 131
SaqAI TTAA 5 cut(s) 1005, 1061, 1272, 2028, 2327
Sau3AI GATC 9 cut(s) 91, 121, 1140, 1316, 1392, 1449, 2180, 2358, 2526
Sau96I GGNCC 5 cut(s) 759, 1858, 1927, 1928, 2381
ScaI AGTACT 1 cut(s) 845
ScrFI CCNGG 5 cut(s) 763, 829, 1163, 1472, 2436
SduI GDGCHC 3 cut(s) 268, 1931, 2284
SexAI ACCWGGT 1 cut(s) 827
SfaNI GCATC 6 cut(s) 419, 468, 497, 2029, 2182, 2380
SfcI CTRYAG 4 cut(s) 541, 1767, 1829, 2316
Sfr274I CTCGAG 1 cut(s) 1946
SfuI TTCGAA 1 cut(s) 626
SinI GGWCC 2 cut(s) 759, 1858
SlaI CTCGAG 1 cut(s) 1946
SmiMI CAYNNNNRTG 5 cut(s) 87, 1040, 1642, 2019, 2398
SmlI CTYRAG 4 cut(s) 507, 1359, 1668, 1946
SmoI CTYRAG 4 cut(s) 507, 1359, 1668, 1946
Sse9I AATT 8 cut(s) 277, 1176, 1213, 1281, 1796, 1889, 1898, 2106
SseBI AGGCCT 2 cut(s) 422, 434
SsiI CCGC 8 cut(s) 249, 284, 513, 858, 1051, 2372, 2407, 2507
SspI AATATT 1 cut(s) 901
SspMI CTAG 9 cut(s) 465, 806, 1122, 1149, 1286, 1595, 1865, 2121, 2385
SstI GAGCTC 2 cut(s) 268, 2284
StuI AGGCCT 2 cut(s) 422, 434
StyD4I CCNGG 5 cut(s) 761, 827, 1161, 1470, 2434
StyI CCWWGG 1 cut(s) 2083
TaaI ACNGT 7 cut(s) 7, 154, 656, 711, 821, 2134, 2426
TaiI ACGT 4 cut(s) 30, 138, 147, 1390
TasI AATT 8 cut(s) 277, 1176, 1213, 1281, 1796, 1889, 1898, 2106
TatI WGTACW 2 cut(s) 773, 843
TauI GCSGC 1 cut(s) 515
TfiI GAWTC 5 cut(s) 322, 1069, 1427, 1825, 1909
Tru1I TTAA 5 cut(s) 1005, 1061, 1272, 2028, 2327
Tru9I TTAA 5 cut(s) 1005, 1061, 1272, 2028, 2327
TscAI CASTG 4 cut(s) 159, 366, 826, 1534
TseFI GTSAC 7 cut(s) 57, 823, 1354, 1663, 2012, 2452, 2536
Tsp45I GTSAC 7 cut(s) 57, 823, 1354, 1663, 2012, 2452, 2536
TspDTI ATGAA 7 cut(s) 509, 966, 1838, 1988, 2046, 2445, 2558
TspRI CASTG 4 cut(s) 159, 366, 826, 1534
VpaK11BI GGWCC 2 cut(s) 759, 1858
XapI RAATTY 2 cut(s) 1176, 1889
XbaI TCTAGA 2 cut(s) 1121, 1285
XceI RCATGY 1 cut(s) 890
XhoI CTCGAG 1 cut(s) 1946
XmiI GTMKAC 2 cut(s) 72, 132
XmnI GAANNNNTTC 1 cut(s) 786
XspI CTAG 9 cut(s) 465, 806, 1122, 1149, 1286, 1595, 1865, 2121, 2385
ZrmI AGTACT 1 cut(s) 845
Zsp2I ATGCAT 2 cut(s) 2005, 2395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.