MD09G1014800.v1.1

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002610
Physical Location & Seq
Forward (+)
936349 .. 940085
3737 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1014800.v1.1.491

Sequence Viewer

Length: 2265 bp
ATGACGGTGTTCTCCGGCAAACAGGTGTTCCCGGTGGACTACGAGGCCGAGGTTTCGCAGCGACTTCTCGAAGCCTCGCTCTCCGGCGACCTGAAATCTGCGCTGGAGTGCGTCGCCGATCCGTTCGTGGACGTCAACTTCGTCGGCGATGTGAGTCTCAAAACGAGAAAGTGTGAGGTGCTGTTGCGCGACGAGTCGGCGAGCGAAGTCCGCGTCGACTACGAGGAGTTCAAAACCGACGTCACGGCTCTGTTCCTCGCCGTTCATGCTGGAAACGTCGCTTTGGTGAAGAAACTGCTGAGCATCGGAGCTGACGTAAATCAGAAACTCTTCAGGGGCTTCGCGACAACGGCAGCAGTGAGAGAGGGCCACCTTGAGATTCTAGAGATCCTACTGAAAGCTGGAGCATCTCAACCGGCTTGTGAGGAAGCTCTATTGGAGGCAAGTTGTCATGGACATGCTACGCTCGTGGAGCGGCTTATGGCCTCTGATTTGATCCGGCCCCATGTTGCTGTGCATGCTATCGTCACAGCCAGCTGCAGGGGGTTTGCTGATGTGGTGGACACTCTCATGAAGTGCGGTGTGGATGCAAGTGCGGCTGATAGGACGCTACTTCAATCGTCTAAGCCAGCTCTTCACACTAATGTTGACTGTAGTGCACTTGTGGCTGCAGTTGTGAGTAGGCAGGTCCCCATCGTACGTTTGCTGCTACAGGCCGGCGTGAGGACGGATGTCAATGTGAGATTGGGAGCATGGTCGTGGGACCCGGCTACGGGGGAAGAGTTGCGGGTTGGGGCAGGGCTGGCGGAGCCTTATCCCATTACCTGGTGTGCAGTAGAGTATTTTGAAGCAAGCGGTTCTGTCTTGCACGTGCTCCTCCAGGGCCGTTCTCCGGATACCCCTCATTGTGGAAGAACTCTCCTGCACCACGCTATTCTATGCGGTAATGGAGGGGCCGTCCGCGTGCTCTTGAGTTGCGGTGCCAATGTAGAATCACCCGTTAAAACAACTGGTAGTACCATGTTTAATCCGATACACATGGCTGCCCGCCTTGGCTTGCCAACGGTCGTTCGATGTTTAATTGAATCGGGATGTGATATGAACTCCAAGACTGATTCCGGTGAGACAGCTCTAATGATCTGCGCAAAATACAAGCATGAAGAATGTCTCAGAGTATTGGCGGCAGCCGGTGCTGATTTTGGCTTGGTTAACGCGGCTGGTCAGTCTGTTAGTTCGATTGCCAGGATAGCTAGGTGGTCCCTTGGTTTTCAGCAGGCGTTGATGTATGTAATAAGAGACGGAAAGATGCCCAAATCCAGCAACTTTTCTGTTTTCTCTCCCCTAATGTTTGCAGCTCAAGCCGGCGATATTGAGGCCTTGAAAGCCGTAGTTGTTTCAGGAGAATTTGACATTGATTATCAAGACGGTAAAGGTTTCACGGCCGTTATGATCACTGCTTTGAAGGGACACGTTGAAGCATTCAGGTTTCTGGTTTATGCAGGGGCTGATGTAAAATTGTGTAACAAGTCTGGGGAGACTGCAATTACTCTGTCAGAAATAAGCCAAAACCGTGACCTGTTCGAGAAGGTAATGCTCGAATATGCACTAGAAAAGGGCAATCTTTACGCTGGAGGGTTCTACGCTCTGCACTGCGCAGCACGCCGTGGAGATGACGATGCAGTCAAATTGTTAACAAGTAGGGGATATGATGTGAATGTCCCTGACGGAGATGGTAACACTCCGCTCATGTTAGCGGCTAGGGAAGGTTATGGTCTCGTGTGTGAACTCTTGATCTCTCACGGAGCAAACGTGGATGTCAAAAATGCCAAAGGCGAAACACCGCTTTCGCTTGCGAGGACAAGCCGCGGTTTGAAAAATGATGCGGAGCGGGTGATACTAGATGAGCTAGCTCGCAAGCTGGTGCTAGGAGGGGCGCTTCTCCGGAAGCATACAAGGGGAGGGAAAGGAAGCCCTCATGGGAAAGAGGTGAGGATGGTGGGAGCTGTAGGGGTGCTGTGCTGGGGGAAGTCAAACCGGAGAAATGTGAAATGCCGGGAGGCGGAGGTGGGGCCTAGTCCAGCCTTTAGGAGGAATAGGAGGAGCCAGAGTGATGCAGATGAACCCGGAGTGTTTAGGGTGGTGACTACCAAGAACAAGGAGGTGCATTTTGTGTCTGAAGGCGGAGTTGAAACCGCCAAGCTTTGGGTGAGAGGTATAAAGCTCGTGACCAAGGAGGCTCTTTTCGGTAGACAGAGGGAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000278 GO:0000281 GO:0000902 GO:0000904 GO:0000910 GO:0001508 GO:0002028 GO:0003008 GO:0003254 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0005829 GO:0005856 GO:0005886 GO:0005911 GO:0006810 GO:0006888 GO:0006892 GO:0006893 GO:0006996 GO:0007009 GO:0007010 GO:0007016 GO:0007049 GO:0007154 GO:0007275 GO:0007399 GO:0007409 GO:0007528 GO:0008092 GO:0008104 GO:0008150 GO:0009653 GO:0009893 GO:0009986 GO:0009987 GO:0010035 GO:0010038 GO:0010256 GO:0010468 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010649 GO:0010650 GO:0010765 GO:0010959 GO:0010960 GO:0012505 GO:0014704 GO:0014731 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0016328 GO:0016528 GO:0016529 GO:0019222 GO:0019226 GO:0019228 GO:0022008 GO:0022402 GO:0022407 GO:0022409 GO:0022607 GO:0022898 GO:0023052 GO:0030016 GO:0030017 GO:0030018 GO:0030030 GO:0030054 GO:0030154 GO:0030155 GO:0030182 GO:0030315 GO:0030424 GO:0030425 GO:0030507 GO:0030674 GO:0031175 GO:0031594 GO:0031674 GO:0032026 GO:0032386 GO:0032388 GO:0032409 GO:0032410 GO:0032411 GO:0032412 GO:0032413 GO:0032414 GO:0032501 GO:0032502 GO:0032507 GO:0032879 GO:0032880 GO:0032989 GO:0032990 GO:0033036 GO:0033157 GO:0033267 GO:0033268 GO:0034110 GO:0034112 GO:0034613 GO:0034762 GO:0034763 GO:0034764 GO:0034765 GO:0034766 GO:0034767 GO:0035637 GO:0036477 GO:0042221 GO:0042383 GO:0042391 GO:0042592 GO:0042886 GO:0042995 GO:0043001 GO:0043005 GO:0043034 GO:0043194 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043266 GO:0043267 GO:0043269 GO:0043270 GO:0043271 GO:0043292 GO:0044085 GO:0044091 GO:0044092 GO:0044093 GO:0044291 GO:0044304 GO:0044325 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044449 GO:0044456 GO:0044459 GO:0044463 GO:0044464 GO:0045184 GO:0045185 GO:0045202 GO:0045211 GO:0045296 GO:0045760 GO:0045785 GO:0045838 GO:0046907 GO:0048193 GO:0048468 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050808 GO:0050839 GO:0050877 GO:0050896 GO:0051049 GO:0051050 GO:0051051 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051235 GO:0051301 GO:0051641 GO:0051649 GO:0051651 GO:0051716 GO:0055065 GO:0055080 GO:0060090 GO:0060255 GO:0060341 GO:0061024 GO:0061564 GO:0061640 GO:0061951 GO:0065007 GO:0065008 GO:0065009 GO:0070201 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071286 GO:0071702 GO:0071705 GO:0071709 GO:0071840 GO:0071944 GO:0072507 GO:0072657 GO:0072658 GO:0072659 GO:0072660 GO:0090087 GO:0090150 GO:0090313 GO:0090314 GO:0090316 GO:0097060 GO:0097447 GO:0097458 GO:0098590 GO:0098771 GO:0098794 GO:0098876 GO:0098900 GO:0098901 GO:0098902 GO:0099080 GO:0099081 GO:0099512 GO:0120025 GO:0120036 GO:0120038 GO:0120039 GO:1900825 GO:1900827 GO:1901016 GO:1901017 GO:1901379 GO:1901380 GO:1902259 GO:1902260 GO:1902305 GO:1902307 GO:1903047 GO:1903533 GO:1903817 GO:1903827 GO:1903829 GO:1904062 GO:1904063 GO:1904064 GO:1904181 GO:1904951 GO:1905475 GO:1905477 GO:1990778 GO:2000649 GO:2000651 GO:2001257 GO:2001258 GO:2001259
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3

Protein Analysis

755

Amino Acids

81.09

Weight (kDa)

7.25

Isoelectric Point (pI)

32.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 79 - 137 5e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 287 - 370 3.4e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 343 - 401 5.8e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 448 - 528 8.5e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 549 - 619 2.9e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 549 - 598 5.1e-08 Ankyrin repeats (many copies)
Ank_5 PF13857 570 - 618 1e-05 Ankyrin repeats (many copies)
Ank PF00023 577 - 608 5.7e-08 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1679
AatII GACGTC 2 cut(s) 135, 243
Acc16I TGCGCA 2 cut(s) 1144, 1654
Acc36I ACCTGC 1 cut(s) 676
AccB1I GGYRCC 1 cut(s) 980
AccB7I CCANNNNNTGG 2 cut(s) 825, 2202
AccBSI CCGCTC 3 cut(s) 475, 1744, 1888
AccI GTMKAC 2 cut(s) 216, 2248
AccII CGCG 6 cut(s) 189, 213, 344, 963, 1214, 1866
AccIII TCCGGA 2 cut(s) 892, 1941
AclWI GGATC 3 cut(s) 113, 382, 490
AcoI YGGCCR 1 cut(s) 1440
AcsI RAATTY 1 cut(s) 1403
AcuI CTGAAG 2 cut(s) 316, 2196
AcvI CACGTG 1 cut(s) 871
AcyI GRCGYC 2 cut(s) 132, 240
AdeI CACNNNGTG 1 cut(s) 1664
AfaI GTAC 2 cut(s) 699, 1018
AflIII ACRYGT 1 cut(s) 1468
AgsI TTSAA 9 cut(s) 232, 617, 848, 1085, 1381, 1462, 1475, 1873, 2189
AjnI CCWGG 3 cut(s) 824, 879, 1241
Alw21I GWGCWC 3 cut(s) 661, 876, 969
Alw26I GTCTC 6 cut(s) 161, 1118, 1172, 1290, 1529, 1778
Alw44I GTGCAC 1 cut(s) 657
AlwI GGATC 3 cut(s) 113, 382, 490
AlwNI CAGNNNCTG 1 cut(s) 1505
Aor13HI TCCGGA 2 cut(s) 892, 1941
ApaLI GTGCAC 1 cut(s) 657
ApeKI GCWGC 9 cut(s) 58, 353, 537, 668, 706, 1043, 1184, 1352, 1655
ApoI RAATTY 1 cut(s) 1403
ArsI GACNNNNNNTTYG 6 cut(s) 122, 154, 198, 225, 230, 257
Asp700I GAANNNNTTC 1 cut(s) 329
AspLEI GCGC 5 cut(s) 103, 189, 1145, 1655, 1936
AspS9I GGNCC 8 cut(s) 367, 501, 688, 763, 883, 954, 1257, 2069
AsuC2I CCSGG 4 cut(s) 32, 767, 2054, 2124
AsuHPI GGTGA 7 cut(s) 298, 987, 1133, 1903, 1999, 2152, 2218
AsuNHI GCTAGC 1 cut(s) 1906
AvaII GGWCC 3 cut(s) 688, 763, 1257
BaeGI GKGCMC 1 cut(s) 661
BanI GGYRCC 1 cut(s) 980
BauI CACGAG 3 cut(s) 467, 1775, 2222
BbrPI CACGTG 1 cut(s) 871
Bbv12I GWGCWC 3 cut(s) 661, 876, 969
BbvI GCAGC 9 cut(s) 70, 365, 524, 655, 693, 1030, 1196, 1364, 1667
BccI CCATC 3 cut(s) 701, 1724, 1987
BceAI ACGGC 9 cut(s) 245, 261, 366, 870, 941, 1370, 1427, 1455, 1647
BciT130I CCWGG 3 cut(s) 826, 881, 1243
BciVI GTATCC 1 cut(s) 889
BclI TGATCA 1 cut(s) 1449
BcnI CCSGG 4 cut(s) 32, 767, 2054, 2124
BcoDI GTCTC 6 cut(s) 161, 1118, 1172, 1290, 1529, 1778
BfaI CTAG 8 cut(s) 383, 1251, 1607, 1758, 1898, 1907, 1925, 2073
BfmI CTRYAG 5 cut(s) 538, 652, 669, 710, 2004
BfoI RGCGCY 1 cut(s) 1937
BfuAI ACCTGC 1 cut(s) 676
BfuI GTATCC 1 cut(s) 889
BlpI GCTNAGC 1 cut(s) 299
Bme1390I CCNGG 7 cut(s) 32, 767, 826, 881, 1243, 2054, 2124
Bme18I GGWCC 3 cut(s) 688, 763, 1257
BmgT120I GGNCC 8 cut(s) 367, 501, 688, 763, 883, 954, 1257, 2069
BmrFI CCNGG 7 cut(s) 32, 767, 826, 881, 1243, 2054, 2124
BmsI GCATC 7 cut(s) 312, 416, 577, 1296, 1666, 1870, 2101
BmtI GCTAGC 1 cut(s) 1910
BoxI GACNNNNGTC 1 cut(s) 731
BpmI CTGGAG 4 cut(s) 125, 423, 863, 1650
Bpu1102I GCTNAGC 1 cut(s) 299
BpuEI CTTGAG 3 cut(s) 395, 991, 1341
BpuMI CCSGG 4 cut(s) 32, 767, 2054, 2124
BsaAI YACGTR 1 cut(s) 871
BsaHI GRCGYC 2 cut(s) 132, 240
BsaI GGTCTC 1 cut(s) 1778
BsaJI CCNNGG 7 cut(s) 48, 880, 1051, 1261, 1663, 1864, 2229
BsaWI WCCGGW 4 cut(s) 892, 1118, 1941, 2034
BsaXI ACNNNNNCTCC 8 cut(s) 300, 330, 942, 972, 1794, 1824, 2092, 2122
Bse118I RCCGGY 4 cut(s) 415, 716, 1187, 1361
Bse1I ACTGG 1 cut(s) 1015
BseAI TCCGGA 2 cut(s) 892, 1941
BseBI CCWGG 3 cut(s) 826, 881, 1243
BseDI CCNNGG 7 cut(s) 48, 880, 1051, 1261, 1663, 1864, 2229
BseGI GGATG 5 cut(s) 592, 736, 1097, 1819, 1998
BseMII CTCAG 2 cut(s) 290, 1183
BseNI ACTGG 1 cut(s) 1015
BseRI GAGGAG 3 cut(s) 239, 866, 2113
BseSI GKGCMC 1 cut(s) 661
BseX3I CGGCCG 1 cut(s) 1440
BseXI GCAGC 9 cut(s) 70, 365, 524, 655, 693, 1030, 1196, 1364, 1667
BseYI CCCAGC 1 cut(s) 2019
BsgI GTGCAG 3 cut(s) 852, 908, 1631
Bsh1236I CGCG 6 cut(s) 189, 213, 344, 963, 1214, 1866
Bsh1285I CGRYCG 2 cut(s) 1068, 1443
BshNI GGYRCC 1 cut(s) 980
BsiEI CGRYCG 2 cut(s) 1068, 1443
BsiHKAI GWGCWC 3 cut(s) 661, 876, 969
BsiWI CGTACG 1 cut(s) 697
BslFI GGGAC 5 cut(s) 674, 776, 1243, 1479, 1703
BsmAI GTCTC 6 cut(s) 161, 1118, 1172, 1290, 1529, 1778
BsmBI CGTCTC 1 cut(s) 1290
BsmFI GGGAC 5 cut(s) 674, 776, 1243, 1479, 1703
BsmI GAATGC 1 cut(s) 1478
Bso31I GGTCTC 1 cut(s) 1778
Bsp1286I GDGCHC 3 cut(s) 661, 876, 969
Bsp13I TCCGGA 2 cut(s) 892, 1941
Bsp143I GATC 6 cut(s) 118, 387, 495, 1137, 1449, 1791
Bsp1720I GCTNAGC 1 cut(s) 299
Bsp68I TCGCGA 1 cut(s) 344
BspCNI CTCAG 2 cut(s) 291, 1182
BspEI TCCGGA 2 cut(s) 892, 1941
BspFNI CGCG 6 cut(s) 189, 213, 344, 963, 1214, 1866
BspHI TCATGA 1 cut(s) 570
BspMAI CTGCAG 2 cut(s) 542, 673
BspMI ACCTGC 1 cut(s) 676
BspOI GCTAGC 1 cut(s) 1910
BspPI GGATC 3 cut(s) 113, 382, 490
BspQI GCTCTTC 1 cut(s) 639
BspT107I GGYRCC 1 cut(s) 980
BspTNI GGTCTC 1 cut(s) 1778
BsrBI CCGCTC 3 cut(s) 475, 1744, 1888
BsrFI RCCGGY 4 cut(s) 415, 716, 1187, 1361
BsrI ACTGG 1 cut(s) 1015
BssAI RCCGGY 4 cut(s) 415, 716, 1187, 1361
BssECI CCNNGG 7 cut(s) 48, 880, 1051, 1261, 1663, 1864, 2229
BssMI GATC 6 cut(s) 118, 387, 495, 1137, 1449, 1791
BssNI GRCGYC 2 cut(s) 132, 240
BssSI CACGAG 3 cut(s) 467, 1775, 2222
BssT1I CCWWGG 3 cut(s) 1051, 1261, 2229
Bst2BI CACGAG 3 cut(s) 467, 1775, 2222
Bst2UI CCWGG 3 cut(s) 826, 881, 1243
Bst4CI ACNGT 5 cut(s) 7, 653, 1066, 1427, 1571
Bst6I CTCTTC 3 cut(s) 335, 639, 774
BstACI GRCGYC 2 cut(s) 132, 240
BstAPI GCANNNNNTGC 1 cut(s) 1190
BstBAI YACGTR 1 cut(s) 871
BstDEI CTNAG 3 cut(s) 299, 624, 1169
BstDSI CCRYGG 2 cut(s) 1663, 1864
BstENI CCTNNNNNAGG 1 cut(s) 2086
BstF5I GGATG 5 cut(s) 592, 736, 1097, 1819, 1998
BstFNI CGCG 6 cut(s) 189, 213, 344, 963, 1214, 1866
BstH2I RGCGCY 1 cut(s) 1937
BstHHI GCGC 5 cut(s) 103, 189, 1145, 1655, 1936
BstKTI GATC 6 cut(s) 121, 390, 498, 1140, 1452, 1794
BstMAI GTCTC 6 cut(s) 161, 1118, 1172, 1290, 1529, 1778
BstMBI GATC 6 cut(s) 118, 387, 495, 1137, 1449, 1791
BstMCI CGRYCG 2 cut(s) 1068, 1443
BstNI CCWGG 3 cut(s) 826, 881, 1243
BstNSI RCATGY 2 cut(s) 461, 521
BstPAI GACNNNNGTC 1 cut(s) 731
BstSCI CCNGG 7 cut(s) 30, 765, 824, 879, 1241, 2052, 2122
BstSFI CTRYAG 5 cut(s) 538, 652, 669, 710, 2004
BstSLI GKGCMC 1 cut(s) 661
BstUI CGCG 6 cut(s) 189, 213, 344, 963, 1214, 1866
BstV1I GCAGC 9 cut(s) 70, 365, 524, 655, 693, 1030, 1196, 1364, 1667
BstX2I RGATCY 1 cut(s) 387
BstYI RGATCY 1 cut(s) 387
BstZI CGGCCG 1 cut(s) 1440
BsuI GTATCC 1 cut(s) 889
BtgI CCRYGG 2 cut(s) 1663, 1864
BtgZI GCGATG 1 cut(s) 162
BtsCI GGATG 5 cut(s) 592, 736, 1097, 1819, 1998
BtsI GCAGTG 3 cut(s) 363, 1452, 1648
BtsIMutI CAGTG 3 cut(s) 363, 1452, 1648
BtuMI TCGCGA 1 cut(s) 344
BveI ACCTGC 1 cut(s) 676
CaiI CAGNNNCTG 1 cut(s) 1505
CciI TCATGA 1 cut(s) 570
CfoI GCGC 5 cut(s) 103, 189, 1145, 1655, 1936
Cfr10I RCCGGY 4 cut(s) 415, 716, 1187, 1361
Cfr13I GGNCC 8 cut(s) 367, 501, 688, 763, 883, 954, 1257, 2069
Cfr42I CCGCGG 1 cut(s) 1867
CseI GACGC 3 cut(s) 100, 202, 616
CsiI ACCWGGT 1 cut(s) 824
Csp6I GTAC 2 cut(s) 698, 1017
CviQI GTAC 2 cut(s) 698, 1017
DdeI CTNAG 3 cut(s) 299, 624, 1169
DpnI GATC 6 cut(s) 120, 389, 497, 1139, 1451, 1793
DpnII GATC 6 cut(s) 118, 387, 495, 1137, 1449, 1791
DraIII CACNNNGTG 1 cut(s) 1664
DrdI GACNNNNNNGTC 1 cut(s) 1679
DseDI GACNNNNNNGTC 1 cut(s) 1679
EaeI YGGCCR 1 cut(s) 1440
EagI CGGCCG 1 cut(s) 1440
Eam1104I CTCTTC 3 cut(s) 335, 639, 774
EarI CTCTTC 3 cut(s) 335, 639, 774
EciI GGCGGA 3 cut(s) 821, 2075, 2196
EclXI CGGCCG 1 cut(s) 1440
Eco130I CCWWGG 3 cut(s) 1051, 1261, 2229
Eco147I AGGCCT 1 cut(s) 1376
Eco31I GGTCTC 1 cut(s) 1778
Eco47I GGWCC 3 cut(s) 688, 763, 1257
Eco52I CGGCCG 1 cut(s) 1440
Eco57I CTGAAG 2 cut(s) 316, 2196
Eco72I CACGTG 1 cut(s) 871
EcoNI CCTNNNNNAGG 1 cut(s) 2086
EcoO109I RGGNCCY 3 cut(s) 688, 763, 2069
EcoRII CCWGG 3 cut(s) 824, 879, 1241
EcoT14I CCWWGG 3 cut(s) 1051, 1261, 2229
ErhI CCWWGG 3 cut(s) 1051, 1261, 2229
Esp3I CGTCTC 1 cut(s) 1290
FaqI GGGAC 5 cut(s) 674, 776, 1243, 1479, 1703
FauI CCCGC 3 cut(s) 780, 1055, 1881
FbaI TGATCA 1 cut(s) 1449
FblI GTMKAC 2 cut(s) 216, 2248
FokI GGATG 5 cut(s) 599, 743, 1104, 1826, 2005
FspBI CTAG 8 cut(s) 383, 1251, 1607, 1758, 1898, 1907, 1925, 2073
FspI TGCGCA 2 cut(s) 1144, 1654
GlaI GCGC 5 cut(s) 102, 188, 1144, 1654, 1935
GsaI CCCAGC 1 cut(s) 2023
GsuI CTGGAG 4 cut(s) 125, 423, 863, 1650
HaeII RGCGCY 1 cut(s) 1937
HgaI GACGC 3 cut(s) 100, 202, 616
HhaI GCGC 5 cut(s) 103, 189, 1145, 1655, 1936
Hin1I GRCGYC 2 cut(s) 132, 240
Hin6I GCGC 5 cut(s) 101, 187, 1143, 1653, 1934
HinP1I GCGC 5 cut(s) 101, 187, 1143, 1653, 1934
HincII GTYRAC 5 cut(s) 136, 217, 649, 1210, 1692
HindII GTYRAC 5 cut(s) 136, 217, 649, 1210, 1692
HindIII AAGCTT 1 cut(s) 2198
HinfI GANTC 6 cut(s) 154, 194, 379, 992, 1085, 1115
HpaI GTTAAC 2 cut(s) 1210, 1692
HphI GGTGA 7 cut(s) 298, 987, 1133, 1903, 1999, 2152, 2218
Hpy188I TCNGA 7 cut(s) 308, 324, 490, 1032, 1172, 1555, 2176
Hpy99I CGWCG 6 cut(s) 116, 146, 194, 218, 242, 281
HpyAV CCTTC 4 cut(s) 1456, 1579, 1757, 2171
HpyCH4III ACNGT 5 cut(s) 7, 653, 1066, 1427, 1571
HpyCH4IV ACGT 8 cut(s) 132, 240, 276, 315, 700, 870, 1470, 1809
HpyF3I CTNAG 3 cut(s) 299, 624, 1169
HpySE526I ACGT 8 cut(s) 132, 240, 276, 315, 700, 870, 1470, 1809
Hsp92I GRCGYC 2 cut(s) 132, 240
HspAI GCGC 5 cut(s) 101, 187, 1143, 1653, 1934
KflI GGGWCCC 1 cut(s) 763
Kpn2I TCCGGA 2 cut(s) 892, 1941
KroI GCCGGC 2 cut(s) 716, 1361
KroNI GCCGGC 2 cut(s) 718, 1363
Ksp22I TGATCA 1 cut(s) 1449
KspAI GTTAAC 2 cut(s) 1210, 1692
KspI CCGCGG 1 cut(s) 1867
Kzo9I GATC 6 cut(s) 118, 387, 495, 1137, 1449, 1791
LguI GCTCTTC 1 cut(s) 639
Lsp1109I GCAGC 9 cut(s) 70, 365, 524, 655, 693, 1030, 1196, 1364, 1667
LweI GCATC 7 cut(s) 312, 416, 577, 1296, 1666, 1870, 2101
MabI ACCWGGT 1 cut(s) 824
MaeI CTAG 8 cut(s) 383, 1251, 1607, 1758, 1898, 1907, 1925, 2073
MaeII ACGT 8 cut(s) 132, 240, 276, 315, 700, 870, 1470, 1809
MaeIII GTNAC 7 cut(s) 241, 526, 1520, 1571, 1733, 2140, 2224
MalI GATC 6 cut(s) 120, 389, 497, 1139, 1451, 1793
MbiI CCGCTC 3 cut(s) 475, 1744, 1888
MboI GATC 6 cut(s) 118, 387, 495, 1137, 1449, 1791
MboII GAAGA 6 cut(s) 301, 322, 626, 791, 924, 1172
MflI RGATCY 1 cut(s) 387
MhlI GDGCHC 3 cut(s) 661, 876, 969
MluCI AATT 5 cut(s) 1080, 1403, 1514, 1542, 1685
MlyI GAGTC 2 cut(s) 163, 203
MroI TCCGGA 2 cut(s) 892, 1941
MroNI GCCGGC 2 cut(s) 716, 1361
MroXI GAANNNNTTC 1 cut(s) 329
MseI TTAA 5 cut(s) 1002, 1026, 1079, 1209, 1691
MslI CAYNNNNRTG 4 cut(s) 456, 569, 642, 757
MspA1I CMGCKG 2 cut(s) 537, 1866
MspR9I CCNGG 7 cut(s) 32, 767, 826, 881, 1243, 2054, 2124
Mva1269I GAATGC 1 cut(s) 1478
MvaI CCWGG 3 cut(s) 826, 881, 1243
MvnI CGCG 6 cut(s) 189, 213, 344, 963, 1214, 1866
NaeI GCCGGC 2 cut(s) 718, 1363
NciI CCSGG 4 cut(s) 32, 767, 2054, 2124
NdeII GATC 6 cut(s) 118, 387, 495, 1137, 1449, 1791
NgoMIV GCCGGC 2 cut(s) 716, 1361
NheI GCTAGC 1 cut(s) 1906
NmeAIII GCCGAG 1 cut(s) 73
NmuCI GTSAC 5 cut(s) 241, 526, 1571, 2140, 2224
NruI TCGCGA 1 cut(s) 344
NsbI TGCGCA 2 cut(s) 1144, 1654
NspI RCATGY 2 cut(s) 461, 521
PaeI GCATGC 1 cut(s) 521
PagI TCATGA 1 cut(s) 570
PceI AGGCCT 1 cut(s) 1376
PciSI GCTCTTC 1 cut(s) 639
PcsI WCGNNNNNNNCGW 3 cut(s) 138, 312, 1806
PctI GAATGC 1 cut(s) 1478
PdiI GCCGGC 2 cut(s) 718, 1363
PdmI GAANNNNTTC 1 cut(s) 329
PfeI GAWTC 4 cut(s) 379, 992, 1085, 1115
Pfl23II CGTACG 1 cut(s) 697
PflMI CCANNNNNTGG 2 cut(s) 825, 2202
PleI GAGTC 2 cut(s) 162, 202
PmaCI CACGTG 1 cut(s) 871
PmlI CACGTG 1 cut(s) 871
PpsI GAGTC 2 cut(s) 162, 202
Ppu21I YACGTR 1 cut(s) 871
PpuMI RGGWCCY 2 cut(s) 688, 763
PshAI GACNNNNGTC 1 cut(s) 731
Psp5II RGGWCCY 2 cut(s) 688, 763
Psp6I CCWGG 3 cut(s) 824, 879, 1241
PspCI CACGTG 1 cut(s) 871
PspFI CCCAGC 1 cut(s) 2019
PspGI CCWGG 3 cut(s) 824, 879, 1241
PspLI CGTACG 1 cut(s) 697
PspPI GGNCC 8 cut(s) 367, 501, 688, 763, 883, 954, 1257, 2069
PspPPI RGGWCCY 2 cut(s) 688, 763
PstI CTGCAG 2 cut(s) 542, 673
PstNI CAGNNNCTG 1 cut(s) 1505
PsuI RGATCY 1 cut(s) 387
PvuII CAGCTG 1 cut(s) 537
RruI TCGCGA 1 cut(s) 344
RsaI GTAC 2 cut(s) 699, 1018
RsaNI GTAC 2 cut(s) 698, 1017
RseI CAYNNNNRTG 4 cut(s) 456, 569, 642, 757
SacII CCGCGG 1 cut(s) 1867
SalI GTCGAC 1 cut(s) 215
SapI GCTCTTC 1 cut(s) 639
SaqAI TTAA 5 cut(s) 1002, 1026, 1079, 1209, 1691
Sau3AI GATC 6 cut(s) 118, 387, 495, 1137, 1449, 1791
Sau96I GGNCC 8 cut(s) 367, 501, 688, 763, 883, 954, 1257, 2069
SchI GAGTC 2 cut(s) 163, 203
ScrFI CCNGG 7 cut(s) 32, 767, 826, 881, 1243, 2054, 2124
SduI GDGCHC 3 cut(s) 661, 876, 969
SexAI ACCWGGT 1 cut(s) 824
SfaNI GCATC 7 cut(s) 312, 416, 577, 1296, 1666, 1870, 2101
SfcI CTRYAG 5 cut(s) 538, 652, 669, 710, 2004
Sfr303I CCGCGG 1 cut(s) 1867
SgrBI CCGCGG 1 cut(s) 1867
SinI GGWCC 3 cut(s) 688, 763, 1257
SmiMI CAYNNNNRTG 4 cut(s) 456, 569, 642, 757
SmlI CTYRAG 3 cut(s) 374, 970, 1356
SmoI CTYRAG 3 cut(s) 374, 970, 1356
SphI GCATGC 1 cut(s) 521
Sse9I AATT 5 cut(s) 1080, 1403, 1514, 1542, 1685
SseBI AGGCCT 1 cut(s) 1376
SspMI CTAG 8 cut(s) 383, 1251, 1607, 1758, 1898, 1907, 1925, 2073
StuI AGGCCT 1 cut(s) 1376
StyD4I CCNGG 7 cut(s) 30, 765, 824, 879, 1241, 2052, 2122
StyI CCWWGG 3 cut(s) 1051, 1261, 2229
TaaI ACNGT 5 cut(s) 7, 653, 1066, 1427, 1571
TaiI ACGT 8 cut(s) 135, 243, 279, 318, 703, 873, 1473, 1812
TaqI TCGA 6 cut(s) 69, 216, 1072, 1235, 1581, 1596
TasI AATT 5 cut(s) 1080, 1403, 1514, 1542, 1685
TauI GCSGC 6 cut(s) 478, 599, 1184, 1217, 1757, 1866
TfiI GAWTC 4 cut(s) 379, 992, 1085, 1115
Tru1I TTAA 5 cut(s) 1002, 1026, 1079, 1209, 1691
Tru9I TTAA 5 cut(s) 1002, 1026, 1079, 1209, 1691
TscAI CASTG 3 cut(s) 363, 1459, 1655
TseFI GTSAC 5 cut(s) 241, 526, 1571, 2140, 2224
TseI GCWGC 9 cut(s) 58, 353, 537, 668, 706, 1043, 1184, 1352, 1655
Tsp45I GTSAC 5 cut(s) 241, 526, 1571, 2140, 2224
TspDTI ATGAA 5 cut(s) 254, 587, 1115, 1173, 2133
TspGWI ACGGA 5 cut(s) 111, 743, 1314, 1740, 1815
TspRI CASTG 3 cut(s) 363, 1459, 1655
Van91I CCANNNNNTGG 2 cut(s) 825, 2202
VneI GTGCAC 1 cut(s) 657
VpaK11BI GGWCC 3 cut(s) 688, 763, 1257
XagI CCTNNNNNAGG 1 cut(s) 2086
XapI RAATTY 1 cut(s) 1403
XbaI TCTAGA 1 cut(s) 382
XceI RCATGY 2 cut(s) 461, 521
XmiI GTMKAC 2 cut(s) 216, 2248
XmnI GAANNNNTTC 1 cut(s) 329
XspI CTAG 8 cut(s) 383, 1251, 1607, 1758, 1898, 1907, 1925, 2073
ZraI GACGTC 2 cut(s) 133, 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.