Rw2G054190

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002610
Physical Location & Seq
Reverse (-)
81861567 .. 81866200
4634 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G054190.1

Sequence Viewer

Length: 2253 bp
ATGACGGTTTTCTCCAGCAAACAGGTGTTCCCGGTGGACTGCGAGGCCGAGGTTTCGCAGCGCCTTCTGGAAGCCTCGCTCGCCGGAGATCTGAAGTCGGCGATGGACTGCGCCGCCGATCCTTTCGTCGACGTCAACTTCGTCGGCGCCGTGTGCCTGAAATGCAGGAGGACTGAGGTGGTGCTGCGGGACGAATCGGCCAGCGAAGTCCGAGTCGGTTACGAGGAGTTCAAAACTGACGTCACGGCTCTCTTCGTCGCCGTTCATGGCGGAAATGTTGAATTGGTGAAGAAATTGCTGAGCATTGGAGCTGATGTAAATCAGCAGCTCTTCCGTGGCTTCGCGACAACAGCAGCAGTGAGAGAGGGCCATCTTGGGATTCTCGAGATCCTACTTAAAGCTGGAGCATCTCAACCAGCTTGTGAGGAAGCTCTGTTGGAGGCAAGTTGTCATGGAAATGCTAAAAACGTGGAGCTGCTCATGTCCTCTGACTTGATCCGGCCCCATCTTGCTGTACATGCTATTGTCATAGCATGCTGCAGGGGGTTTGTGGATGTGGTTGACACTCTCATGAAGTGTGGTGTCGATGCAAGTGCAGTCGATAGGATACTGCTTCAGTCATCTAAGCCTTCTCTGCACACCAATGTCGACTGTAGTGCACTGGTAGCTGCAGTTGTTAGCAGGCAGGTTGCTACTGTCCGTTTGCTGCTAAAGGCTGGTGCAAGGACAGATGTCCAAGTGAGGCTGGGAGCATGGTCATGGGACATAGCTACAGGGGAAGAGTTGCGCGTTGGTGCAGGACTGGCCGAACCTTATCCCATTACTTGGTGTGCAGTGGAATACTTTGAAGCTAGTGGTTCAATCTTGCACTTGCTCCTCCAAAATATTTCCCCTAATACCCCTTATTGCGGAAGAACTCTCCTGCACCATGCCGTCCTATGTGGCAATGTAGGAGCTGTGCGTGCGCTACTACATTGTGGTGCCAATGTAGAAGCGCCTGTTAAAACAACTGGGAAGACAATGTTCAATGCAATACACATGGCCGCCCGCCTTGGTTTGTCAGCAGTCCTTCAATGTTTGATCAATTCTGGCTCTGATATAAACTCCAAGACAGACTCTGGTGAGACAGCTCTAATGATCTGTGCAAAATATAAACATGAAGAGTGTCTCAGAGTATTGGCCATGGCTGGTGCTGATTTTGGCTTGGTCAATGTTGCTGCTCAGTCTGTCAGTTCAATTGCTGTCACAAACCGGTGGTCCCTTGGTTTTCAGGAGGCATTGGTAGATATAATAAGAATCGGCAAGATACCTAAATCCAGCAACTTTTCTGTTTTCTCTCCCCTGATGTTTGTCGCTCAAGCTGGTGATATTGAGGCCTTGAAAGCTATAGTTGATTCAGGAGAATTTGAGATTGATTATCAGGATGATAAAGGTTTTAGTCCAGTCATGATCACTGCTTTGGAGGGTCACGTTGAAGCATTCCAGTTGCTGGTTTATGCAGGAGCTGATGTAAAGTTGTGCAACAAATCTGGTGAGACTGCAATTACTCTATCAGCATTAAGCCAAAACCGTGACCTATTTGAGAAGGTGATGCTTGAATTTGCACTCGAAAAGGGTAATCGCTATGCTGGGGGTTTCTATGCTCTACATTGTGCTGCACGTCGTGGGGACATGGATGCAGCCAAGTTGTTAACAGGTAGGGGTTACGATGTAAATGTCCCTGATGGAGATGGTTACACACCTCTCATGTTAGCAGCCAGGGAAGGCTATGGTACCATGTGTGAACTCTTGATCTCTCATGGGGCCAAGCTAGAGGTCATGAATGCTAAAGGCGAAACGCCACTCTCACTTGCAAGGAAAAGGGGTGGTTTGAAAAATGATGCTGAGCGTGTGATTCTGGATGAACTTGCTCGCAAGCTGGTGCTACGTGGGGCTCGGGTTCTCAAGCACACAAAGGGCGGGAAAGGAAGTCCTCATGAGAAAGATCTGAGAATGGTGGGGAGTGAAGGGGTGCTGCGATGGGGCAAGTCAAATCAGAGAAATGTGATATGCCGAGAGGCTGAGGTGAGAGCTAGCCCGGCTTTTATCAGGAACAGGGGGAGCAAGAGTGATGTCAATGATGCTGGAGTGTTTAGGGTTGTGACTACCAAAAACAAGGAGGTGCATTTTGTGTGCGAAGGTGGGGTTGAAATGGCCGAGCTTTGGGTGAGGGGTATAACACTTGTAACCAAAGAGGCTGGCTATTTTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000278 GO:0000281 GO:0000902 GO:0000904 GO:0000910 GO:0001508 GO:0002028 GO:0003008 GO:0003254 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0005829 GO:0005856 GO:0005886 GO:0005911 GO:0006810 GO:0006888 GO:0006892 GO:0006893 GO:0006996 GO:0007009 GO:0007010 GO:0007016 GO:0007049 GO:0007154 GO:0007275 GO:0007399 GO:0007409 GO:0007528 GO:0008092 GO:0008104 GO:0008150 GO:0009653 GO:0009893 GO:0009986 GO:0009987 GO:0010035 GO:0010038 GO:0010256 GO:0010468 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010649 GO:0010650 GO:0010765 GO:0010959 GO:0010960 GO:0012505 GO:0014704 GO:0014731 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0016328 GO:0016528 GO:0016529 GO:0019222 GO:0019226 GO:0019228 GO:0022008 GO:0022402 GO:0022407 GO:0022409 GO:0022607 GO:0022898 GO:0023052 GO:0030016 GO:0030017 GO:0030018 GO:0030030 GO:0030054 GO:0030154 GO:0030155 GO:0030182 GO:0030315 GO:0030424 GO:0030425 GO:0030507 GO:0030674 GO:0031175 GO:0031594 GO:0031674 GO:0032026 GO:0032386 GO:0032388 GO:0032409 GO:0032410 GO:0032411 GO:0032412 GO:0032413 GO:0032414 GO:0032501 GO:0032502 GO:0032507 GO:0032879 GO:0032880 GO:0032989 GO:0032990 GO:0033036 GO:0033157 GO:0033267 GO:0033268 GO:0034110 GO:0034112 GO:0034613 GO:0034762 GO:0034763 GO:0034764 GO:0034765 GO:0034766 GO:0034767 GO:0035637 GO:0036477 GO:0042221 GO:0042383 GO:0042391 GO:0042592 GO:0042886 GO:0042995 GO:0043001 GO:0043005 GO:0043034 GO:0043194 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043266 GO:0043267 GO:0043269 GO:0043270 GO:0043271 GO:0043292 GO:0044085 GO:0044091 GO:0044092 GO:0044093 GO:0044291 GO:0044304 GO:0044325 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044449 GO:0044456 GO:0044459 GO:0044463 GO:0044464 GO:0045184 GO:0045185 GO:0045202 GO:0045211 GO:0045296 GO:0045760 GO:0045785 GO:0045838 GO:0046907 GO:0048193 GO:0048468 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050808 GO:0050839 GO:0050877 GO:0050896 GO:0051049 GO:0051050 GO:0051051 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051235 GO:0051301 GO:0051641 GO:0051649 GO:0051651 GO:0051716 GO:0055065 GO:0055080 GO:0060090 GO:0060255 GO:0060341 GO:0061024 GO:0061564 GO:0061640 GO:0061951 GO:0065007 GO:0065008 GO:0065009 GO:0070201 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071286 GO:0071702 GO:0071705 GO:0071709 GO:0071840 GO:0071944 GO:0072507 GO:0072657 GO:0072658 GO:0072659 GO:0072660 GO:0090087 GO:0090150 GO:0090313 GO:0090314 GO:0090316 GO:0097060 GO:0097447 GO:0097458 GO:0098590 GO:0098771 GO:0098794 GO:0098876 GO:0098900 GO:0098901 GO:0098902 GO:0099080 GO:0099081 GO:0099512 GO:0120025 GO:0120036 GO:0120038 GO:0120039 GO:1900825 GO:1900827 GO:1901016 GO:1901017 GO:1901379 GO:1901380 GO:1902259 GO:1902260 GO:1902305 GO:1902307 GO:1903047 GO:1903533 GO:1903817 GO:1903827 GO:1903829 GO:1904062 GO:1904063 GO:1904064 GO:1904181 GO:1904951 GO:1905475 GO:1905477 GO:1990778 GO:2000649 GO:2000651 GO:2001257 GO:2001258 GO:2001259
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3

Protein Analysis

750

Amino Acids

80.72

Weight (kDa)

6.44

Isoelectric Point (pI)

27.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 343 - 401 9.8e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 448 - 528 3.3e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 487 - 575 2.3e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 549 - 621 2.4e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 549 - 598 8.8e-09 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 135, 243
Acc36I ACCTGC 1 cut(s) 676
Acc65I GGTACC 1 cut(s) 1772
AccB1I GGYRCC 3 cut(s) 146, 980, 1772
AccB7I CCANNNNNTGG 2 cut(s) 825, 1489
AccI GTMKAC 2 cut(s) 129, 648
AccII CGCG 2 cut(s) 344, 789
AciI CCGC 7 cut(s) 114, 187, 270, 909, 1044, 1048, 1959
AclWI GGATC 3 cut(s) 113, 382, 490
AcoI YGGCCR 5 cut(s) 198, 804, 1041, 1179, 2193
AcsI RAATTY 2 cut(s) 1403, 1598
AcuI CTGAAG 2 cut(s) 113, 599
AcyI GRCGYC 3 cut(s) 132, 147, 240
AdeI CACNNNGTG 1 cut(s) 1664
AfaI GTAC 2 cut(s) 516, 1774
AfiI CCNNNNNNNGG 4 cut(s) 825, 908, 1489, 2202
AgeI ACCGGT 1 cut(s) 1251
AjiI CACGTC 1 cut(s) 1661
AjnI CCWGG 1 cut(s) 1757
Alw21I GWGCWC 1 cut(s) 661
Alw26I GTCTC 3 cut(s) 1118, 1172, 1529
Alw44I GTGCAC 1 cut(s) 657
AlwI GGATC 3 cut(s) 113, 382, 490
AlwNI CAGNNNCTG 3 cut(s) 1118, 1489, 1505
Ama87I CYCGRG 2 cut(s) 383, 1935
ApaLI GTGCAC 1 cut(s) 657
ApoI RAATTY 2 cut(s) 1403, 1598
ArsI GACNNNNNNTTYG 8 cut(s) 122, 154, 198, 225, 230, 257, 1241, 1273
AsiGI ACCGGT 1 cut(s) 1251
Asp718I GGTACC 1 cut(s) 1772
AspLEI GCGC 6 cut(s) 63, 113, 149, 789, 967, 997
AspS9I GGNCC 4 cut(s) 367, 501, 1257, 1803
AsuC2I CCSGG 2 cut(s) 32, 2078
AsuHPI GGTGA 7 cut(s) 298, 1133, 1376, 1544, 1600, 2077, 2218
AsuNHI GCTAGC 1 cut(s) 2072
AvaI CYCGRG 2 cut(s) 383, 1935
AvaII GGWCC 1 cut(s) 1257
BaeGI GKGCMC 1 cut(s) 661
BalI TGGCCA 1 cut(s) 1181
BanI GGYRCC 3 cut(s) 146, 980, 1772
BanII GRGCYC 1 cut(s) 1936
BbsI GAAGAC 1 cut(s) 1022
Bbv12I GWGCWC 1 cut(s) 661
BbvCI CCTCAGC 1 cut(s) 2061
BccI CCATC 6 cut(s) 97, 378, 513, 1718, 1724, 2013
BceAI ACGGC 4 cut(s) 134, 245, 261, 917
BcgI CGANNNNNNTGC 4 cut(s) 575, 609, 638, 672
BciT130I CCWGG 1 cut(s) 1759
BciVI GTATCC 1 cut(s) 600
BclI TGATCA 2 cut(s) 1080, 1449
BcnI CCSGG 2 cut(s) 32, 2078
BcoDI GTCTC 3 cut(s) 1118, 1172, 1529
BfaI CTAG 3 cut(s) 852, 1811, 2073
BfmI CTRYAG 5 cut(s) 538, 652, 669, 771, 1386
BfoI RGCGCY 3 cut(s) 64, 150, 998
BfuAI ACCTGC 1 cut(s) 676
BfuI GTATCC 1 cut(s) 600
BglII AGATCT 2 cut(s) 88, 1984
BlpI GCTNAGC 2 cut(s) 299, 1884
Bme1390I CCNGG 3 cut(s) 32, 1759, 2078
Bme18I GGWCC 1 cut(s) 1257
BmeT110I CYCGRG 2 cut(s) 383, 1935
BmgBI CACGTC 1 cut(s) 1661
BmgT120I GGNCC 4 cut(s) 367, 501, 1257, 1803
BmiI GGNNCC 6 cut(s) 148, 503, 982, 1259, 1774, 1804
BmrFI CCNGG 3 cut(s) 32, 1759, 2078
BmrI ACTGGG 1 cut(s) 1020
BmsI GCATC 6 cut(s) 416, 577, 1581, 1666, 1870, 2110
BmtI GCTAGC 1 cut(s) 2076
BmuI ACTGGG 1 cut(s) 1020
BoxI GACNNNNGTC 1 cut(s) 731
BpiI GAAGAC 1 cut(s) 1022
BpmI CTGGAG 2 cut(s) 423, 2145
Bpu10I CCTNAGC 1 cut(s) 2061
Bpu1102I GCTNAGC 2 cut(s) 299, 1884
BpuEI CTTGAG 2 cut(s) 1341, 1928
BpuMI CCSGG 2 cut(s) 32, 2078
BsaAI YACGTR 1 cut(s) 1928
BsaBI GATNNNNATC 1 cut(s) 318
BsaHI GRCGYC 3 cut(s) 132, 147, 240
BsaJI CCNNGG 6 cut(s) 48, 334, 1051, 1182, 1261, 1758
BsaWI WCCGGW 1 cut(s) 1251
BsaXI ACNNNNNCTCC 6 cut(s) 300, 330, 1494, 1524, 2092, 2122
Bsc4I CCNNNNNNNGG 4 cut(s) 825, 908, 1489, 2202
Bse118I RCCGGY 1 cut(s) 1251
Bse1I ACTGG 5 cut(s) 666, 807, 1015, 1442, 1483
Bse3DI GCAATG 1 cut(s) 952
Bse8I GATNNNNATC 1 cut(s) 318
BseBI CCWGG 1 cut(s) 1759
BseDI CCNNGG 6 cut(s) 48, 334, 1051, 1182, 1261, 1758
BseGI GGATG 4 cut(s) 559, 1429, 1681, 1906
BseJI GATNNNNATC 1 cut(s) 318
BseLI CCNNNNNNNGG 4 cut(s) 825, 908, 1489, 2202
BseMI GCAATG 1 cut(s) 952
BseMII CTCAG 7 cut(s) 165, 290, 1183, 1235, 1875, 1979, 2052
BseNI ACTGG 5 cut(s) 666, 807, 1015, 1442, 1483
BseRI GAGGAG 2 cut(s) 239, 866
BseSI GKGCMC 1 cut(s) 661
BseYI CCCAGC 2 cut(s) 745, 1628
BsgI GTGCAG 6 cut(s) 615, 620, 816, 852, 908, 1641
Bsh1236I CGCG 2 cut(s) 344, 789
BshNI GGYRCC 3 cut(s) 146, 980, 1772
BshTI ACCGGT 1 cut(s) 1251
BsiHKAI GWGCWC 1 cut(s) 661
BsiHKCI CYCGRG 2 cut(s) 383, 1935
BsiSI CCGG 5 cut(s) 32, 84, 499, 1252, 2078
BslFI GGGAC 5 cut(s) 203, 776, 1243, 1682, 1703
BslI CCNNNNNNNGG 4 cut(s) 825, 908, 1489, 2202
BsmAI GTCTC 3 cut(s) 1118, 1172, 1529
BsmFI GGGAC 5 cut(s) 203, 776, 1243, 1682, 1703
BsmI GAATGC 2 cut(s) 1478, 1828
BsoBI CYCGRG 2 cut(s) 383, 1935
Bsp1286I GDGCHC 2 cut(s) 661, 1936
Bsp1407I TGTACA 1 cut(s) 514
Bsp143I GATC 9 cut(s) 88, 118, 387, 495, 1080, 1137, 1449, 1791, 1984
Bsp1720I GCTNAGC 2 cut(s) 299, 1884
Bsp19I CCATGG 1 cut(s) 1182
Bsp68I TCGCGA 1 cut(s) 344
BspACI CCGC 7 cut(s) 114, 187, 270, 909, 1044, 1048, 1959
BspCNI CTCAG 7 cut(s) 166, 291, 1182, 1234, 1876, 1980, 2053
BspFNI CGCG 2 cut(s) 344, 789
BspHI TCATGA 4 cut(s) 570, 1446, 1818, 1975
BspLI GGNNCC 6 cut(s) 148, 503, 982, 1259, 1774, 1804
BspMAI CTGCAG 2 cut(s) 542, 673
BspMI ACCTGC 1 cut(s) 676
BspOI GCTAGC 1 cut(s) 2076
BspPI GGATC 3 cut(s) 113, 382, 490
BspQI GCTCTTC 1 cut(s) 335
BspT107I GGYRCC 3 cut(s) 146, 980, 1772
BsrDI GCAATG 1 cut(s) 952
BsrFI RCCGGY 1 cut(s) 1251
BsrGI TGTACA 1 cut(s) 514
BsrI ACTGG 5 cut(s) 666, 807, 1015, 1442, 1483
BssAI RCCGGY 1 cut(s) 1251
BssECI CCNNGG 6 cut(s) 48, 334, 1051, 1182, 1261, 1758
BssMI GATC 9 cut(s) 88, 118, 387, 495, 1080, 1137, 1449, 1791, 1984
BssNI GRCGYC 3 cut(s) 132, 147, 240
BssT1I CCWWGG 3 cut(s) 1051, 1182, 1261
Bst2UI CCWGG 1 cut(s) 1759
Bst4CI ACNGT 4 cut(s) 7, 653, 697, 1571
Bst6I CTCTTC 4 cut(s) 257, 335, 774, 1155
BstACI GRCGYC 3 cut(s) 132, 147, 240
BstAUI TGTACA 1 cut(s) 514
BstBAI YACGTR 1 cut(s) 1928
BstDEI CTNAG 8 cut(s) 174, 299, 624, 1169, 1221, 1884, 1988, 2061
BstDSI CCRYGG 2 cut(s) 334, 1182
BstF5I GGATG 4 cut(s) 559, 1429, 1681, 1906
BstFNI CGCG 2 cut(s) 344, 789
BstH2I RGCGCY 3 cut(s) 64, 150, 998
BstHHI GCGC 6 cut(s) 63, 113, 149, 789, 967, 997
BstKTI GATC 9 cut(s) 91, 121, 390, 498, 1083, 1140, 1452, 1794, 1987
BstMAI GTCTC 3 cut(s) 1118, 1172, 1529
BstMBI GATC 9 cut(s) 88, 118, 387, 495, 1080, 1137, 1449, 1791, 1984
BstNI CCWGG 1 cut(s) 1759
BstNSI RCATGY 2 cut(s) 521, 537
BstPAI GACNNNNGTC 1 cut(s) 731
BstSCI CCNGG 3 cut(s) 30, 1757, 2076
BstSFI CTRYAG 5 cut(s) 538, 652, 669, 771, 1386
BstSLI GKGCMC 1 cut(s) 661
BstUI CGCG 2 cut(s) 344, 789
BstV2I GAAGAC 1 cut(s) 1022
BstX2I RGATCY 3 cut(s) 88, 387, 1984
BstYI RGATCY 3 cut(s) 88, 387, 1984
BsuI GTATCC 1 cut(s) 600
BtgI CCRYGG 2 cut(s) 334, 1182
BtgZI GCGATG 2 cut(s) 116, 2032
BtrI CACGTC 1 cut(s) 1661
BtsCI GGATG 4 cut(s) 559, 1429, 1681, 1906
BtsI GCAGTG 3 cut(s) 363, 840, 1452
BtsIMutI CAGTG 4 cut(s) 363, 659, 840, 1452
BtuMI TCGCGA 1 cut(s) 344
BveI ACCTGC 1 cut(s) 676
CaiI CAGNNNCTG 3 cut(s) 1118, 1489, 1505
CciI TCATGA 4 cut(s) 570, 1446, 1818, 1975
CfoI GCGC 6 cut(s) 63, 113, 149, 789, 967, 997
Cfr10I RCCGGY 1 cut(s) 1251
Cfr13I GGNCC 4 cut(s) 367, 501, 1257, 1803
Csp6I GTAC 2 cut(s) 515, 1773
CspAI ACCGGT 1 cut(s) 1251
CviQI GTAC 2 cut(s) 515, 1773
DdeI CTNAG 8 cut(s) 174, 299, 624, 1169, 1221, 1884, 1988, 2061
DinI GGCGCC 1 cut(s) 148
DpnI GATC 9 cut(s) 90, 120, 389, 497, 1082, 1139, 1451, 1793, 1986
DpnII GATC 9 cut(s) 88, 118, 387, 495, 1080, 1137, 1449, 1791, 1984
DraIII CACNNNGTG 1 cut(s) 1664
EaeI YGGCCR 5 cut(s) 198, 804, 1041, 1179, 2193
Eam1104I CTCTTC 4 cut(s) 257, 335, 774, 1155
EarI CTCTTC 4 cut(s) 257, 335, 774, 1155
EciI GGCGGA 1 cut(s) 285
Eco130I CCWWGG 3 cut(s) 1051, 1182, 1261
Eco147I AGGCCT 1 cut(s) 1376
Eco24I GRGCYC 1 cut(s) 1936
Eco47I GGWCC 1 cut(s) 1257
Eco57I CTGAAG 2 cut(s) 113, 599
Eco88I CYCGRG 2 cut(s) 383, 1935
EcoRII CCWGG 1 cut(s) 1757
EcoT14I CCWWGG 3 cut(s) 1051, 1182, 1261
EcoT38I GRGCYC 1 cut(s) 1936
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
ErhI CCWWGG 3 cut(s) 1051, 1182, 1261
FaqI GGGAC 5 cut(s) 203, 776, 1243, 1682, 1703
FauI CCCGC 3 cut(s) 180, 1055, 1952
FbaI TGATCA 2 cut(s) 1080, 1449
FblI GTMKAC 2 cut(s) 129, 648
FokI GGATG 4 cut(s) 566, 1436, 1688, 1913
FriOI GRGCYC 1 cut(s) 1936
FspBI CTAG 3 cut(s) 852, 1811, 2073
GlaI GCGC 6 cut(s) 62, 112, 148, 788, 966, 996
GsaI CCCAGC 2 cut(s) 749, 1632
GsuI CTGGAG 2 cut(s) 423, 2145
HaeII RGCGCY 3 cut(s) 64, 150, 998
HapII CCGG 5 cut(s) 32, 84, 499, 1252, 2078
HhaI GCGC 6 cut(s) 63, 113, 149, 789, 967, 997
Hin1I GRCGYC 3 cut(s) 132, 147, 240
Hin6I GCGC 6 cut(s) 61, 111, 147, 787, 965, 995
HinP1I GCGC 6 cut(s) 61, 111, 147, 787, 965, 995
HincII GTYRAC 5 cut(s) 130, 136, 562, 649, 1692
HindII GTYRAC 5 cut(s) 130, 136, 562, 649, 1692
HinfI GANTC 7 cut(s) 194, 213, 379, 1115, 1296, 1394, 1894
HpaI GTTAAC 1 cut(s) 1692
HpaII CCGG 5 cut(s) 32, 84, 499, 1252, 2078
HphI GGTGA 7 cut(s) 298, 1133, 1376, 1544, 1600, 2077, 2218
Hpy166II GTNNAC 8 cut(s) 37, 130, 136, 562, 649, 659, 1692, 1784
Hpy188I TCNGA 7 cut(s) 93, 212, 490, 1096, 1172, 1989, 2037
Hpy8I GTNNAC 8 cut(s) 37, 130, 136, 562, 649, 659, 1692, 1784
Hpy99I CGWCG 5 cut(s) 131, 134, 146, 260, 1665
HpyAV CCTTC 7 cut(s) 74, 639, 1079, 1579, 1757, 2000, 2171
HpyCH4III ACNGT 4 cut(s) 7, 653, 697, 1571
HpyCH4IV ACGT 6 cut(s) 132, 240, 468, 1470, 1660, 1927
HpyF3I CTNAG 8 cut(s) 174, 299, 624, 1169, 1221, 1884, 1988, 2061
HpySE526I ACGT 6 cut(s) 132, 240, 468, 1470, 1660, 1927
Hsp92I GRCGYC 3 cut(s) 132, 147, 240
HspAI GCGC 6 cut(s) 61, 111, 147, 787, 965, 995
KasI GGCGCC 1 cut(s) 146
KpnI GGTACC 1 cut(s) 1776
Ksp22I TGATCA 2 cut(s) 1080, 1449
KspAI GTTAAC 1 cut(s) 1692
Kzo9I GATC 9 cut(s) 88, 118, 387, 495, 1080, 1137, 1449, 1791, 1984
LguI GCTCTTC 1 cut(s) 335
LmnI GCTCC 8 cut(s) 308, 404, 472, 749, 879, 953, 1502, 2100
LweI GCATC 6 cut(s) 416, 577, 1581, 1666, 1870, 2110
MaeI CTAG 3 cut(s) 852, 1811, 2073
MaeII ACGT 6 cut(s) 132, 240, 468, 1470, 1660, 1927
MaeIII GTNAC 9 cut(s) 218, 241, 1243, 1466, 1571, 1703, 1733, 2140, 2224
MalI GATC 9 cut(s) 90, 120, 389, 497, 1082, 1139, 1451, 1793, 1986
MboI GATC 9 cut(s) 88, 118, 387, 495, 1080, 1137, 1449, 1791, 1984
MboII GAAGA 7 cut(s) 244, 301, 322, 791, 924, 1027, 1172
MfeI CAATTG 1 cut(s) 1236
MflI RGATCY 3 cut(s) 88, 387, 1984
MhlI GDGCHC 2 cut(s) 661, 1936
MlsI TGGCCA 1 cut(s) 1181
MluCI AATT 7 cut(s) 281, 293, 1084, 1236, 1403, 1542, 1598
MluNI TGGCCA 1 cut(s) 1181
Mly113I GGCGCC 1 cut(s) 147
MlyI GAGTC 2 cut(s) 222, 1109
MmeI TCCRAC 1 cut(s) 417
Mox20I TGGCCA 1 cut(s) 1181
MscI TGGCCA 1 cut(s) 1181
MseI TTAA 4 cut(s) 396, 1002, 1559, 1691
MslI CAYNNNNRTG 5 cut(s) 456, 569, 642, 757, 978
Msp20I TGGCCA 1 cut(s) 1181
MspI CCGG 5 cut(s) 32, 84, 499, 1252, 2078
MspR9I CCNGG 3 cut(s) 32, 1759, 2078
MunI CAATTG 1 cut(s) 1236
Mva1269I GAATGC 2 cut(s) 1478, 1828
MvaI CCWGG 1 cut(s) 1759
MvnI CGCG 2 cut(s) 344, 789
NarI GGCGCC 1 cut(s) 147
NciI CCSGG 2 cut(s) 32, 2078
NcoI CCATGG 1 cut(s) 1182
NdeII GATC 9 cut(s) 88, 118, 387, 495, 1080, 1137, 1449, 1791, 1984
NheI GCTAGC 1 cut(s) 2072
NlaIV GGNNCC 6 cut(s) 148, 503, 982, 1259, 1774, 1804
NmeAIII GCCGAG 3 cut(s) 73, 2078, 2221
NmuCI GTSAC 5 cut(s) 241, 1243, 1466, 1571, 2140
NruI TCGCGA 1 cut(s) 344
NspI RCATGY 2 cut(s) 521, 537
PaeI GCATGC 1 cut(s) 537
PaeR7I CTCGAG 1 cut(s) 383
PagI TCATGA 4 cut(s) 570, 1446, 1818, 1975
PceI AGGCCT 1 cut(s) 1376
PciSI GCTCTTC 1 cut(s) 335
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 147
PctI GAATGC 2 cut(s) 1478, 1828
PfeI GAWTC 5 cut(s) 194, 379, 1296, 1394, 1894
PflMI CCANNNNNTGG 2 cut(s) 825, 1489
PinAI ACCGGT 1 cut(s) 1251
PleI GAGTC 2 cut(s) 221, 1109
PluTI GGCGCC 1 cut(s) 150
PpsI GAGTC 2 cut(s) 221, 1109
Ppu21I YACGTR 1 cut(s) 1928
PshAI GACNNNNGTC 1 cut(s) 731
Psp6I CCWGG 1 cut(s) 1757
PspFI CCCAGC 2 cut(s) 745, 1628
PspGI CCWGG 1 cut(s) 1757
PspN4I GGNNCC 6 cut(s) 148, 503, 982, 1259, 1774, 1804
PspPI GGNCC 4 cut(s) 367, 501, 1257, 1803
PstI CTGCAG 2 cut(s) 542, 673
PstNI CAGNNNCTG 3 cut(s) 1118, 1489, 1505
PsuI RGATCY 3 cut(s) 88, 387, 1984
RruI TCGCGA 1 cut(s) 344
RsaI GTAC 2 cut(s) 516, 1774
RsaNI GTAC 2 cut(s) 515, 1773
RseI CAYNNNNRTG 5 cut(s) 456, 569, 642, 757, 978
SalI GTCGAC 2 cut(s) 128, 647
SapI GCTCTTC 1 cut(s) 335
SaqAI TTAA 4 cut(s) 396, 1002, 1559, 1691
Sau3AI GATC 9 cut(s) 88, 118, 387, 495, 1080, 1137, 1449, 1791, 1984
Sau96I GGNCC 4 cut(s) 367, 501, 1257, 1803
SchI GAGTC 2 cut(s) 222, 1109
ScrFI CCNGG 3 cut(s) 32, 1759, 2078
SduI GDGCHC 2 cut(s) 661, 1936
SfaNI GCATC 6 cut(s) 416, 577, 1581, 1666, 1870, 2110
SfcI CTRYAG 5 cut(s) 538, 652, 669, 771, 1386
SfoI GGCGCC 1 cut(s) 148
Sfr274I CTCGAG 1 cut(s) 383
SgrDI CGTCGACG 1 cut(s) 128
SinI GGWCC 1 cut(s) 1257
SlaI CTCGAG 1 cut(s) 383
SmiMI CAYNNNNRTG 5 cut(s) 456, 569, 642, 757, 978
SmlI CTYRAG 3 cut(s) 383, 1356, 1943
SmoI CTYRAG 3 cut(s) 383, 1356, 1943
SphI GCATGC 1 cut(s) 537
Sse9I AATT 7 cut(s) 281, 293, 1084, 1236, 1403, 1542, 1598
SseBI AGGCCT 1 cut(s) 1376
SsiI CCGC 7 cut(s) 114, 187, 270, 909, 1044, 1048, 1959
SspDI GGCGCC 1 cut(s) 146
SspI AATATT 1 cut(s) 886
SspMI CTAG 3 cut(s) 852, 1811, 2073
StuI AGGCCT 1 cut(s) 1376
StyD4I CCNGG 3 cut(s) 30, 1757, 2076
StyI CCWWGG 3 cut(s) 1051, 1182, 1261
TaaI ACNGT 4 cut(s) 7, 653, 697, 1571
TaiI ACGT 6 cut(s) 135, 243, 471, 1473, 1663, 1930
TaqI TCGA 6 cut(s) 129, 384, 585, 600, 648, 1608
TasI AATT 7 cut(s) 281, 293, 1084, 1236, 1403, 1542, 1598
TatI WGTACW 1 cut(s) 514
TauI GCSGC 2 cut(s) 116, 1046
TfiI GAWTC 5 cut(s) 194, 379, 1296, 1394, 1894
Tru1I TTAA 4 cut(s) 396, 1002, 1559, 1691
Tru9I TTAA 4 cut(s) 396, 1002, 1559, 1691
TscAI CASTG 4 cut(s) 363, 666, 840, 1459
TseFI GTSAC 5 cut(s) 241, 1243, 1466, 1571, 2140
Tsp45I GTSAC 5 cut(s) 241, 1243, 1466, 1571, 2140
TspDTI ATGAA 5 cut(s) 254, 587, 1173, 1835, 1917
TspGWI ACGGA 2 cut(s) 323, 689
TspRI CASTG 4 cut(s) 363, 666, 840, 1459
Van91I CCANNNNNTGG 2 cut(s) 825, 1489
VneI GTGCAC 1 cut(s) 657
VpaK11BI GGWCC 1 cut(s) 1257
XapI RAATTY 2 cut(s) 1403, 1598
XceI RCATGY 2 cut(s) 521, 537
XhoI CTCGAG 1 cut(s) 383
XmiI GTMKAC 2 cut(s) 129, 648
XspI CTAG 3 cut(s) 852, 1811, 2073
ZraI GACGTC 2 cut(s) 133, 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.