pycom111g01210

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0002610
Physical Location & Seq
Forward (+)
782489 .. 786204
3716 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g01210.4

Sequence Viewer

Length: 2265 bp
ATGACGGTGTTCTCCGGCAAACAGGTGGTCCCGGTGGACTACGAGGCCGAGGTTTCGCAGCGACTTCTCGAAGCCTCGCTCTCCGGCGACCTGAAATCGGCGTTGGAGTGCGCCGCCGATCCTTTCGTGGACGTCAACTTCGTCGGCGCTGTGAGTCTCAAAACGAGAAAGTGTGAGGTATTGTTGCGCGACGAGTCTGCGAGCGAAGTCCGCATCGACTACGAGGAGTTCAAAACCGACGTCACGGCTCTGTTCCTCGCCGTTCATGCTGGAAACGTCGCTTTGGTGAAGAAACTGCTGAGCATCGGAGCTGACGTAAATCAGAAACTCTTTAGGGGCTTCGCGACAACAGCAGCAGTGAGAGAGGGCCACCTTGAGATTCTAGAGATCCTACTGAAAGCTGGAGCATCTCAACCAGCTTGTGAGGAAGCTCTGTTCGAGGCAAGTTGTCATGGACATGCTAGGCTCGTGGAGCGGCTTATGGCCTCTGATTTTATCCGGCCCCATGTTGCCGTGCATGCCATTGTCACAGCCAGCTGCAGGGGGTTTGCTGATGTGGTGGACACTCTCATGAAGTGCGGTGTGGATGCAAGTGCGGCTGATAGGACGCTACTTCAATCGTCTAAGCCAGCTCTTCACACTAATGTGGACTGTGGTGCACTTGTGGCTGCAGTTGTGAGTAGGCAGGTCCCCATCGTACGTTTGTTGCTACAGGCCGGCGTGGGGACAGATGTCAATGTGAGATTGGGAGCATGGTCATGGGACTCAGCTACGGGGGAAGAGTTGCGGGTTGGGGCAGGGCTTGCGGAGCCATATCCCATTACCTGGTGTGCAGTGGAGTATTTTGAAGCAAGCGGTTCTGTCTTGCACATGCTCCTCCAAGGCCGTTCTCCGGATACCCCTCATTGTGGAAGAACTCTCCTGCACCACGCTATTCTATGCGGTAATGCAGGAGCAGTCCGTTCGCTCTTAAGTTGCGGTGCCAATGTAGAATCACCCGTTAAAACAACTGGTAGTACCATGTTTAATCCGATACACATGGCTGCCCGCCTTGGCTTGCCAACAGTCGTTCGATGTTTAATTGAATCGGGATGTGATGTGAACTCCAATACTGATACCGGCGAGACAGCTCTAATGATTTGCGCAAAATACAAGCATGAAGAATGTCTCAGAGTATTGGCGGCAGCTGGTGCTGATTTTGGCTTGGCTAACGCAGCTGGTCAGTCTGTTAGTTCGATTGCCAGGATAGCTGGGTGGTCCCTTGGTTTTCAGCAGGCGTTGATGTATGTAATAAGAGACGGAAAGATGCCCAAATCCAGCAACTTTTCTGTTTTCTCTCCCCTAATGTTTGCAGCTCAAGCCGGCGATATTGAGGCCTTGAAAGCCGTAGTTGTTTCAGGAGAATTTGACATTGATTATCAAGACGGTAAAGGTTTCACAGCCGTTATGATCACTGCTTTGAAGGGACACGTTGAAGCATTCAGGTTTTTGGTTTATGCAGGGGCTGATGTAAAATTGTGTAACAAGTCTGGTGAGACTGCAATTACTCTGTCAGAAATAAGCCAAAACCGTGACCTGTTTGAGAAGGTAATGCTCGAATATGCGCTAGAAAAGGGCAATCGTTACGCTGGAGGGTTCTACGCTCTGCACTGCGCAGCACGCCGTGGGGATGTCGATGCAGTCAAATTGTTAACAAGTAGGGGATATGATGTGAATGTCCCTGACGGAGATGGTAACACTCCGCTCATGTTAGCGGCTAGGGAAGGTTATGGTCTCGTGTGTGAACTCTTGATCTCTCACGGAGCAAACTTGGATGTCAAGAATGCCAAAGGCGAAACACCGCTTTCGCTTGCTACAAAAAGCGGCGGTTTGGAAAATGATGCGGAGCGGGTGATACTAGATGAGCTAGCTCGCAAGCTGGTGCTAGGTGGGGCGCATGTCAGGAAGCATACAAAAGGAGGGAAAGGAAGCCCTCATGGGAAAGAGGTGAGGATGGTGGGAGCTGTAGGGGTGCTGTGCTGGGGGAAGTCAAACCGGAGAAATGTGAAATGCCGGGAGGCGGAGGTTGGGCCTAGTCCAGCCTTTAAGAGGAATAGGAGGAGCCAGAGTGATGCAGATGAACCTGGAGTGTTTAGGGTGGTGACTACCAAGAACAAGGAGGTGCATTTTGTGTGTGAAGGCGGAGTTGAAACCGCCAAGCTTTGGGTGAGAGGTATAAAGCTCGTGACCAAGGAGGCTCTTTTCGGTAAACAGAGGGGCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000278 GO:0000281 GO:0000902 GO:0000904 GO:0000910 GO:0001508 GO:0002028 GO:0003008 GO:0003254 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0005829 GO:0005856 GO:0005886 GO:0005911 GO:0006810 GO:0006888 GO:0006892 GO:0006893 GO:0006996 GO:0007009 GO:0007010 GO:0007016 GO:0007049 GO:0007154 GO:0007275 GO:0007399 GO:0007409 GO:0007528 GO:0008092 GO:0008104 GO:0008150 GO:0009653 GO:0009893 GO:0009986 GO:0009987 GO:0010035 GO:0010038 GO:0010256 GO:0010468 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010649 GO:0010650 GO:0010765 GO:0010959 GO:0010960 GO:0012505 GO:0014704 GO:0014731 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0016328 GO:0016528 GO:0016529 GO:0019222 GO:0019226 GO:0019228 GO:0022008 GO:0022402 GO:0022407 GO:0022409 GO:0022607 GO:0022898 GO:0023052 GO:0030016 GO:0030017 GO:0030018 GO:0030030 GO:0030054 GO:0030154 GO:0030155 GO:0030182 GO:0030315 GO:0030424 GO:0030425 GO:0030507 GO:0030674 GO:0031175 GO:0031594 GO:0031674 GO:0032026 GO:0032386 GO:0032388 GO:0032409 GO:0032410 GO:0032411 GO:0032412 GO:0032413 GO:0032414 GO:0032501 GO:0032502 GO:0032507 GO:0032879 GO:0032880 GO:0032989 GO:0032990 GO:0033036 GO:0033157 GO:0033267 GO:0033268 GO:0034110 GO:0034112 GO:0034613 GO:0034762 GO:0034763 GO:0034764 GO:0034765 GO:0034766 GO:0034767 GO:0035637 GO:0036477 GO:0042221 GO:0042383 GO:0042391 GO:0042592 GO:0042886 GO:0042995 GO:0043001 GO:0043005 GO:0043034 GO:0043194 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043266 GO:0043267 GO:0043269 GO:0043270 GO:0043271 GO:0043292 GO:0044085 GO:0044091 GO:0044092 GO:0044093 GO:0044291 GO:0044304 GO:0044325 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044449 GO:0044456 GO:0044459 GO:0044463 GO:0044464 GO:0045184 GO:0045185 GO:0045202 GO:0045211 GO:0045296 GO:0045760 GO:0045785 GO:0045838 GO:0046907 GO:0048193 GO:0048468 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050808 GO:0050839 GO:0050877 GO:0050896 GO:0051049 GO:0051050 GO:0051051 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051235 GO:0051301 GO:0051641 GO:0051649 GO:0051651 GO:0051716 GO:0055065 GO:0055080 GO:0060090 GO:0060255 GO:0060341 GO:0061024 GO:0061564 GO:0061640 GO:0061951 GO:0065007 GO:0065008 GO:0065009 GO:0070201 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071286 GO:0071702 GO:0071705 GO:0071709 GO:0071840 GO:0071944 GO:0072507 GO:0072657 GO:0072658 GO:0072659 GO:0072660 GO:0090087 GO:0090150 GO:0090313 GO:0090314 GO:0090316 GO:0097060 GO:0097447 GO:0097458 GO:0098590 GO:0098771 GO:0098794 GO:0098876 GO:0098900 GO:0098901 GO:0098902 GO:0099080 GO:0099081 GO:0099512 GO:0120025 GO:0120036 GO:0120038 GO:0120039 GO:1900825 GO:1900827 GO:1901016 GO:1901017 GO:1901379 GO:1901380 GO:1902259 GO:1902260 GO:1902305 GO:1902307 GO:1903047 GO:1903533 GO:1903817 GO:1903827 GO:1903829 GO:1904062 GO:1904063 GO:1904064 GO:1904181 GO:1904951 GO:1905475 GO:1905477 GO:1990778 GO:2000649 GO:2000651 GO:2001257 GO:2001258 GO:2001259
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3

Protein Analysis

755

Amino Acids

80.6

Weight (kDa)

7.03

Isoelectric Point (pI)

30.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 135, 243
Acc16I TGCGCA 2 cut(s) 1144, 1654
Acc36I ACCTGC 1 cut(s) 676
AccB1I GGYRCC 1 cut(s) 980
AccB7I CCANNNNNTGG 2 cut(s) 825, 2202
AccBSI CCGCTC 3 cut(s) 475, 1744, 1888
AccII CGCG 2 cut(s) 189, 344
AccIII TCCGGA 1 cut(s) 892
AclWI GGATC 2 cut(s) 113, 382
AcsI RAATTY 1 cut(s) 1403
AcyI GRCGYC 2 cut(s) 132, 240
AdeI CACNNNGTG 1 cut(s) 1664
AfaI GTAC 2 cut(s) 699, 1018
AfiI CCNNNNNNNGG 9 cut(s) 97, 540, 723, 825, 892, 908, 2059, 2088, 2202
AflII CTTAAG 1 cut(s) 970
AflIII ACRYGT 1 cut(s) 1468
AgsI TTSAA 8 cut(s) 232, 617, 848, 1085, 1381, 1462, 1475, 2189
AjnI CCWGG 3 cut(s) 824, 1241, 2122
AjuI GAANNNNNNNTTGG 2 cut(s) 86, 118
AleI CACNNNNGTG 1 cut(s) 644
AloI GAACNNNNNNTCC 2 cut(s) 419, 451
Alw21I GWGCWC 1 cut(s) 661
Alw26I GTCTC 6 cut(s) 161, 1118, 1172, 1290, 1529, 1778
Alw44I GTGCAC 1 cut(s) 657
AlwI GGATC 2 cut(s) 113, 382
AlwNI CAGNNNCTG 1 cut(s) 1505
Aor13HI TCCGGA 1 cut(s) 892
AoxI GGCC 8 cut(s) 45, 367, 483, 500, 714, 883, 1374, 2069
ApaLI GTGCAC 1 cut(s) 657
ApeKI GCWGC 9 cut(s) 58, 353, 537, 668, 1043, 1184, 1214, 1352, 1655
ApoI RAATTY 1 cut(s) 1403
ArsI GACNNNNNNTTYG 6 cut(s) 12, 44, 122, 154, 225, 257
AspLEI GCGC 7 cut(s) 113, 149, 189, 1145, 1606, 1655, 1936
AspS9I GGNCC 6 cut(s) 28, 367, 501, 688, 1257, 2069
AsuC2I CCSGG 2 cut(s) 32, 2054
AsuHPI GGTGA 7 cut(s) 298, 987, 1544, 1903, 1999, 2152, 2218
AsuNHI GCTAGC 1 cut(s) 1906
AvaII GGWCC 3 cut(s) 28, 688, 1257
BaeGI GKGCMC 1 cut(s) 661
BanI GGYRCC 1 cut(s) 980
BanII GRGCYC 1 cut(s) 2261
BauI CACGAG 3 cut(s) 467, 1775, 2222
Bbv12I GWGCWC 1 cut(s) 661
BbvI GCAGC 9 cut(s) 70, 365, 524, 655, 1030, 1196, 1226, 1364, 1667
BccI CCATC 3 cut(s) 701, 1724, 1987
BceAI ACGGC 7 cut(s) 245, 261, 497, 870, 1370, 1427, 1647
BcgI CGANNNNNNTGC 4 cut(s) 179, 213, 945, 979
BciT130I CCWGG 3 cut(s) 826, 1243, 2124
BciVI GTATCC 1 cut(s) 889
BclI TGATCA 1 cut(s) 1449
BcnI CCSGG 2 cut(s) 32, 2054
BcoDI GTCTC 6 cut(s) 161, 1118, 1172, 1290, 1529, 1778
BfaI CTAG 8 cut(s) 383, 462, 1607, 1758, 1898, 1907, 1925, 2073
BfmI CTRYAG 4 cut(s) 538, 669, 710, 2004
BfoI RGCGCY 1 cut(s) 150
BfrI CTTAAG 1 cut(s) 970
BfuAI ACCTGC 1 cut(s) 676
BfuI GTATCC 1 cut(s) 889
BlpI GCTNAGC 1 cut(s) 299
Bme1390I CCNGG 5 cut(s) 32, 826, 1243, 2054, 2124
Bme18I GGWCC 3 cut(s) 28, 688, 1257
BmgT120I GGNCC 6 cut(s) 28, 367, 501, 688, 1257, 2069
BmiI GGNNCC 7 cut(s) 30, 503, 690, 810, 982, 1259, 2102
BmrFI CCNGG 5 cut(s) 32, 826, 1243, 2054, 2124
BmsI GCATC 8 cut(s) 222, 312, 416, 577, 1296, 1666, 1870, 2101
BmtI GCTAGC 1 cut(s) 1910
BoxI GACNNNNGTC 1 cut(s) 731
BpmI CTGGAG 3 cut(s) 423, 1650, 2145
Bpu1102I GCTNAGC 1 cut(s) 299
BpuEI CTTGAG 2 cut(s) 395, 1341
BpuMI CCSGG 2 cut(s) 32, 2054
BsaHI GRCGYC 2 cut(s) 132, 240
BsaI GGTCTC 1 cut(s) 1778
BsaJI CCNNGG 6 cut(s) 48, 880, 1051, 1261, 1663, 2229
BsaWI WCCGGW 2 cut(s) 892, 2034
BsaXI ACNNNNNCTCC 4 cut(s) 300, 330, 2092, 2122
Bsc4I CCNNNNNNNGG 9 cut(s) 97, 540, 723, 825, 892, 908, 2059, 2088, 2202
Bse118I RCCGGY 3 cut(s) 716, 1118, 1361
Bse1I ACTGG 1 cut(s) 1015
BseAI TCCGGA 1 cut(s) 892
BseBI CCWGG 3 cut(s) 826, 1243, 2124
BseDI CCNNGG 6 cut(s) 48, 880, 1051, 1261, 1663, 2229
BseGI GGATG 5 cut(s) 592, 1097, 1675, 1819, 1998
BseLI CCNNNNNNNGG 9 cut(s) 97, 540, 723, 825, 892, 908, 2059, 2088, 2202
BseMII CTCAG 3 cut(s) 290, 780, 1183
BseNI ACTGG 1 cut(s) 1015
BseRI GAGGAG 3 cut(s) 239, 866, 2113
BseSI GKGCMC 1 cut(s) 661
BseXI GCAGC 9 cut(s) 70, 365, 524, 655, 1030, 1196, 1226, 1364, 1667
BseYI CCCAGC 2 cut(s) 1250, 2019
BsgI GTGCAG 3 cut(s) 852, 908, 1631
Bsh1236I CGCG 2 cut(s) 189, 344
BshFI GGCC 8 cut(s) 47, 369, 485, 502, 716, 885, 1376, 2071
BshNI GGYRCC 1 cut(s) 980
BsiHKAI GWGCWC 1 cut(s) 661
BsiWI CGTACG 1 cut(s) 697
BslFI GGGAC 7 cut(s) 14, 674, 739, 776, 1243, 1479, 1703
BslI CCNNNNNNNGG 9 cut(s) 97, 540, 723, 825, 892, 908, 2059, 2088, 2202
BsmAI GTCTC 6 cut(s) 161, 1118, 1172, 1290, 1529, 1778
BsmBI CGTCTC 1 cut(s) 1290
BsmFI GGGAC 7 cut(s) 14, 674, 739, 776, 1243, 1479, 1703
BsmI GAATGC 2 cut(s) 1478, 1828
BsnI GGCC 8 cut(s) 47, 369, 485, 502, 716, 885, 1376, 2071
Bso31I GGTCTC 1 cut(s) 1778
Bsp1286I GDGCHC 2 cut(s) 661, 2261
Bsp13I TCCGGA 1 cut(s) 892
Bsp143I GATC 4 cut(s) 118, 387, 1449, 1791
Bsp1720I GCTNAGC 1 cut(s) 299
Bsp68I TCGCGA 1 cut(s) 344
BspANI GGCC 8 cut(s) 47, 369, 485, 502, 716, 885, 1376, 2071
BspCNI CTCAG 3 cut(s) 291, 779, 1182
BspEI TCCGGA 1 cut(s) 892
BspFNI CGCG 2 cut(s) 189, 344
BspHI TCATGA 2 cut(s) 570, 2261
BspLI GGNNCC 7 cut(s) 30, 503, 690, 810, 982, 1259, 2102
BspMAI CTGCAG 2 cut(s) 542, 673
BspMI ACCTGC 1 cut(s) 676
BspOI GCTAGC 1 cut(s) 1910
BspPI GGATC 2 cut(s) 113, 382
BspQI GCTCTTC 1 cut(s) 639
BspT107I GGYRCC 1 cut(s) 980
BspTI CTTAAG 1 cut(s) 970
BspTNI GGTCTC 1 cut(s) 1778
BsrBI CCGCTC 3 cut(s) 475, 1744, 1888
BsrFI RCCGGY 3 cut(s) 716, 1118, 1361
BsrI ACTGG 1 cut(s) 1015
BssAI RCCGGY 3 cut(s) 716, 1118, 1361
BssECI CCNNGG 6 cut(s) 48, 880, 1051, 1261, 1663, 2229
BssMI GATC 4 cut(s) 118, 387, 1449, 1791
BssNI GRCGYC 2 cut(s) 132, 240
BssSI CACGAG 3 cut(s) 467, 1775, 2222
BssT1I CCWWGG 4 cut(s) 880, 1051, 1261, 2229
Bst2BI CACGAG 3 cut(s) 467, 1775, 2222
Bst2UI CCWGG 3 cut(s) 826, 1243, 2124
Bst4CI ACNGT 5 cut(s) 7, 653, 1066, 1427, 1571
Bst6I CTCTTC 2 cut(s) 639, 774
BstACI GRCGYC 2 cut(s) 132, 240
BstAFI CTTAAG 1 cut(s) 970
BstAPI GCANNNNNTGC 2 cut(s) 803, 1190
BstDEI CTNAG 4 cut(s) 299, 624, 766, 1169
BstDSI CCRYGG 1 cut(s) 1663
BstENI CCTNNNNNAGG 1 cut(s) 2086
BstF5I GGATG 5 cut(s) 592, 1097, 1675, 1819, 1998
BstFNI CGCG 2 cut(s) 189, 344
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 7 cut(s) 113, 149, 189, 1145, 1606, 1655, 1936
BstKTI GATC 4 cut(s) 121, 390, 1452, 1794
BstMAI GTCTC 6 cut(s) 161, 1118, 1172, 1290, 1529, 1778
BstMBI GATC 4 cut(s) 118, 387, 1449, 1791
BstNI CCWGG 3 cut(s) 826, 1243, 2124
BstNSI RCATGY 4 cut(s) 461, 521, 874, 1940
BstPAI GACNNNNGTC 1 cut(s) 731
BstSCI CCNGG 5 cut(s) 30, 824, 1241, 2052, 2122
BstSFI CTRYAG 4 cut(s) 538, 669, 710, 2004
BstSLI GKGCMC 1 cut(s) 661
BstUI CGCG 2 cut(s) 189, 344
BstV1I GCAGC 9 cut(s) 70, 365, 524, 655, 1030, 1196, 1226, 1364, 1667
BstX2I RGATCY 1 cut(s) 387
BstYI RGATCY 1 cut(s) 387
BsuI GTATCC 1 cut(s) 889
BsuRI GGCC 8 cut(s) 47, 369, 485, 502, 716, 885, 1376, 2071
BtgI CCRYGG 1 cut(s) 1663
BtsCI GGATG 5 cut(s) 592, 1097, 1675, 1819, 1998
BtsI GCAGTG 4 cut(s) 363, 840, 1452, 1648
BtsIMutI CAGTG 4 cut(s) 363, 840, 1452, 1648
BtuMI TCGCGA 1 cut(s) 344
BveI ACCTGC 1 cut(s) 676
CaiI CAGNNNCTG 1 cut(s) 1505
CciI TCATGA 2 cut(s) 570, 2261
CfoI GCGC 7 cut(s) 113, 149, 189, 1145, 1606, 1655, 1936
Cfr10I RCCGGY 3 cut(s) 716, 1118, 1361
Cfr13I GGNCC 6 cut(s) 28, 367, 501, 688, 1257, 2069
CseI GACGC 1 cut(s) 616
CsiI ACCWGGT 1 cut(s) 824
Csp6I GTAC 2 cut(s) 698, 1017
CviQI GTAC 2 cut(s) 698, 1017
DdeI CTNAG 4 cut(s) 299, 624, 766, 1169
DpnI GATC 4 cut(s) 120, 389, 1451, 1793
DpnII GATC 4 cut(s) 118, 387, 1449, 1791
DraIII CACNNNGTG 1 cut(s) 1664
Eam1104I CTCTTC 2 cut(s) 639, 774
EarI CTCTTC 2 cut(s) 639, 774
EciI GGCGGA 2 cut(s) 2075, 2196
Eco130I CCWWGG 4 cut(s) 880, 1051, 1261, 2229
Eco147I AGGCCT 1 cut(s) 1376
Eco24I GRGCYC 1 cut(s) 2261
Eco31I GGTCTC 1 cut(s) 1778
Eco47I GGWCC 3 cut(s) 28, 688, 1257
EcoNI CCTNNNNNAGG 1 cut(s) 2086
EcoO109I RGGNCCY 1 cut(s) 688
EcoRII CCWGG 3 cut(s) 824, 1241, 2122
EcoT14I CCWWGG 4 cut(s) 880, 1051, 1261, 2229
EcoT38I GRGCYC 1 cut(s) 2261
ErhI CCWWGG 4 cut(s) 880, 1051, 1261, 2229
Esp3I CGTCTC 1 cut(s) 1290
FaqI GGGAC 7 cut(s) 14, 674, 739, 776, 1243, 1479, 1703
FauI CCCGC 3 cut(s) 780, 1055, 1881
FbaI TGATCA 1 cut(s) 1449
FokI GGATG 5 cut(s) 599, 1104, 1682, 1826, 2005
FriOI GRGCYC 1 cut(s) 2261
FspBI CTAG 8 cut(s) 383, 462, 1607, 1758, 1898, 1907, 1925, 2073
FspI TGCGCA 2 cut(s) 1144, 1654
GlaI GCGC 7 cut(s) 112, 148, 188, 1144, 1605, 1654, 1935
GsaI CCCAGC 2 cut(s) 1254, 2023
GsuI CTGGAG 3 cut(s) 423, 1650, 2145
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 8 cut(s) 47, 369, 485, 502, 716, 885, 1376, 2071
HgaI GACGC 1 cut(s) 616
HhaI GCGC 7 cut(s) 113, 149, 189, 1145, 1606, 1655, 1936
Hin1I GRCGYC 2 cut(s) 132, 240
Hin6I GCGC 7 cut(s) 111, 147, 187, 1143, 1604, 1653, 1934
HinP1I GCGC 7 cut(s) 111, 147, 187, 1143, 1604, 1653, 1934
HincII GTYRAC 2 cut(s) 136, 1692
HindII GTYRAC 2 cut(s) 136, 1692
HindIII AAGCTT 1 cut(s) 2198
HinfI GANTC 6 cut(s) 154, 194, 379, 764, 992, 1085
HpaI GTTAAC 1 cut(s) 1692
HphI GGTGA 7 cut(s) 298, 987, 1544, 1903, 1999, 2152, 2218
Hpy188I TCNGA 6 cut(s) 308, 324, 490, 1032, 1172, 1555
Hpy99I CGWCG 4 cut(s) 146, 194, 242, 281
HpyAV CCTTC 4 cut(s) 1456, 1579, 1757, 2171
HpyCH4III ACNGT 5 cut(s) 7, 653, 1066, 1427, 1571
HpyCH4IV ACGT 6 cut(s) 132, 240, 276, 315, 700, 1470
HpyF3I CTNAG 4 cut(s) 299, 624, 766, 1169
HpySE526I ACGT 6 cut(s) 132, 240, 276, 315, 700, 1470
Hsp92I GRCGYC 2 cut(s) 132, 240
HspAI GCGC 7 cut(s) 111, 147, 187, 1143, 1604, 1653, 1934
Kpn2I TCCGGA 1 cut(s) 892
KroI GCCGGC 2 cut(s) 716, 1361
KroNI GCCGGC 2 cut(s) 718, 1363
Ksp22I TGATCA 1 cut(s) 1449
KspAI GTTAAC 1 cut(s) 1692
Kzo9I GATC 4 cut(s) 118, 387, 1449, 1791
LguI GCTCTTC 1 cut(s) 639
Lsp1109I GCAGC 9 cut(s) 70, 365, 524, 655, 1030, 1196, 1226, 1364, 1667
LweI GCATC 8 cut(s) 222, 312, 416, 577, 1296, 1666, 1870, 2101
MabI ACCWGGT 1 cut(s) 824
MaeI CTAG 8 cut(s) 383, 462, 1607, 1758, 1898, 1907, 1925, 2073
MaeII ACGT 6 cut(s) 132, 240, 276, 315, 700, 1470
MaeIII GTNAC 8 cut(s) 241, 526, 1520, 1571, 1622, 1733, 2140, 2224
MalI GATC 4 cut(s) 120, 389, 1451, 1793
MbiI CCGCTC 3 cut(s) 475, 1744, 1888
MboI GATC 4 cut(s) 118, 387, 1449, 1791
MboII GAAGA 5 cut(s) 301, 626, 791, 924, 1172
MflI RGATCY 1 cut(s) 387
MhlI GDGCHC 2 cut(s) 661, 2261
MluCI AATT 5 cut(s) 1080, 1403, 1514, 1542, 1685
MlyI GAGTC 3 cut(s) 163, 203, 758
MmeI TCCRAC 1 cut(s) 84
MroI TCCGGA 1 cut(s) 892
MroNI GCCGGC 2 cut(s) 716, 1361
MseI TTAA 6 cut(s) 971, 1002, 1026, 1079, 1691, 2085
MslI CAYNNNNRTG 5 cut(s) 456, 569, 642, 644, 757
MspA1I CMGCKG 3 cut(s) 537, 1187, 1217
MspCI CTTAAG 1 cut(s) 970
MspR9I CCNGG 5 cut(s) 32, 826, 1243, 2054, 2124
Mva1269I GAATGC 2 cut(s) 1478, 1828
MvaI CCWGG 3 cut(s) 826, 1243, 2124
MvnI CGCG 2 cut(s) 189, 344
NaeI GCCGGC 2 cut(s) 718, 1363
NciI CCSGG 2 cut(s) 32, 2054
NdeII GATC 4 cut(s) 118, 387, 1449, 1791
NgoMIV GCCGGC 2 cut(s) 716, 1361
NheI GCTAGC 1 cut(s) 1906
NlaIV GGNNCC 7 cut(s) 30, 503, 690, 810, 982, 1259, 2102
NmeAIII GCCGAG 1 cut(s) 73
NmuCI GTSAC 5 cut(s) 241, 526, 1571, 2140, 2224
NruI TCGCGA 1 cut(s) 344
NsbI TGCGCA 2 cut(s) 1144, 1654
NspI RCATGY 4 cut(s) 461, 521, 874, 1940
OliI CACNNNNGTG 1 cut(s) 644
PaeI GCATGC 1 cut(s) 521
PagI TCATGA 2 cut(s) 570, 2261
PceI AGGCCT 1 cut(s) 1376
PciSI GCTCTTC 1 cut(s) 639
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 312
PctI GAATGC 2 cut(s) 1478, 1828
PdiI GCCGGC 2 cut(s) 718, 1363
PfeI GAWTC 3 cut(s) 379, 992, 1085
Pfl23II CGTACG 1 cut(s) 697
PflMI CCANNNNNTGG 2 cut(s) 825, 2202
PleI GAGTC 3 cut(s) 162, 202, 758
PpsI GAGTC 3 cut(s) 162, 202, 758
PpuMI RGGWCCY 1 cut(s) 688
PshAI GACNNNNGTC 1 cut(s) 731
Psp5II RGGWCCY 1 cut(s) 688
Psp6I CCWGG 3 cut(s) 824, 1241, 2122
PspFI CCCAGC 2 cut(s) 1250, 2019
PspGI CCWGG 3 cut(s) 824, 1241, 2122
PspLI CGTACG 1 cut(s) 697
PspN4I GGNNCC 7 cut(s) 30, 503, 690, 810, 982, 1259, 2102
PspPI GGNCC 6 cut(s) 28, 367, 501, 688, 1257, 2069
PspPPI RGGWCCY 1 cut(s) 688
PstI CTGCAG 2 cut(s) 542, 673
PstNI CAGNNNCTG 1 cut(s) 1505
PsuI RGATCY 1 cut(s) 387
PvuII CAGCTG 3 cut(s) 537, 1187, 1217
RruI TCGCGA 1 cut(s) 344
RsaI GTAC 2 cut(s) 699, 1018
RsaNI GTAC 2 cut(s) 698, 1017
RseI CAYNNNNRTG 5 cut(s) 456, 569, 642, 644, 757
SapI GCTCTTC 1 cut(s) 639
SaqAI TTAA 6 cut(s) 971, 1002, 1026, 1079, 1691, 2085
Sau3AI GATC 4 cut(s) 118, 387, 1449, 1791
Sau96I GGNCC 6 cut(s) 28, 367, 501, 688, 1257, 2069
SchI GAGTC 3 cut(s) 163, 203, 758
ScrFI CCNGG 5 cut(s) 32, 826, 1243, 2054, 2124
SduI GDGCHC 2 cut(s) 661, 2261
SexAI ACCWGGT 1 cut(s) 824
SfaNI GCATC 8 cut(s) 222, 312, 416, 577, 1296, 1666, 1870, 2101
SfcI CTRYAG 4 cut(s) 538, 669, 710, 2004
SinI GGWCC 3 cut(s) 28, 688, 1257
SmiMI CAYNNNNRTG 5 cut(s) 456, 569, 642, 644, 757
SmlI CTYRAG 3 cut(s) 374, 970, 1356
SmoI CTYRAG 3 cut(s) 374, 970, 1356
SphI GCATGC 1 cut(s) 521
Sse9I AATT 5 cut(s) 1080, 1403, 1514, 1542, 1685
SseBI AGGCCT 1 cut(s) 1376
SspMI CTAG 8 cut(s) 383, 462, 1607, 1758, 1898, 1907, 1925, 2073
StuI AGGCCT 1 cut(s) 1376
StyD4I CCNGG 5 cut(s) 30, 824, 1241, 2052, 2122
StyI CCWWGG 4 cut(s) 880, 1051, 1261, 2229
TaaI ACNGT 5 cut(s) 7, 653, 1066, 1427, 1571
TaiI ACGT 6 cut(s) 135, 243, 279, 318, 703, 1473
TaqI TCGA 7 cut(s) 69, 216, 438, 1072, 1235, 1596, 1674
TasI AATT 5 cut(s) 1080, 1403, 1514, 1542, 1685
TauI GCSGC 6 cut(s) 116, 478, 599, 1184, 1757, 1866
TfiI GAWTC 3 cut(s) 379, 992, 1085
Tru1I TTAA 6 cut(s) 971, 1002, 1026, 1079, 1691, 2085
Tru9I TTAA 6 cut(s) 971, 1002, 1026, 1079, 1691, 2085
TscAI CASTG 4 cut(s) 363, 840, 1459, 1655
TseFI GTSAC 5 cut(s) 241, 526, 1571, 2140, 2224
TseI GCWGC 9 cut(s) 58, 353, 537, 668, 1043, 1184, 1214, 1352, 1655
Tsp45I GTSAC 5 cut(s) 241, 526, 1571, 2140, 2224
TspDTI ATGAA 4 cut(s) 254, 587, 1173, 2133
TspGWI ACGGA 4 cut(s) 950, 1314, 1740, 1815
TspRI CASTG 4 cut(s) 363, 840, 1459, 1655
Van91I CCANNNNNTGG 2 cut(s) 825, 2202
Vha464I CTTAAG 1 cut(s) 970
VneI GTGCAC 1 cut(s) 657
VpaK11BI GGWCC 3 cut(s) 28, 688, 1257
XagI CCTNNNNNAGG 1 cut(s) 2086
XapI RAATTY 1 cut(s) 1403
XbaI TCTAGA 1 cut(s) 382
XceI RCATGY 4 cut(s) 461, 521, 874, 1940
XspI CTAG 8 cut(s) 383, 462, 1607, 1758, 1898, 1907, 1925, 2073
ZraI GACGTC 2 cut(s) 133, 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.