MD17G1014900.v1.1

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002610
Physical Location & Seq
Forward (+)
1237990 .. 1242440
4451 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1014900.v1.1.491

Sequence Viewer

Length: 2262 bp
ATGGTATTCTCCGGCAAACAGGTGTTCCCGGTGGACTACGAGGCCGAGGTTTCGCAACGACTGCTCGAAGCCTCGCTCTCCGGTGATCTGAAATCGGCGCTGGAGTGCATCGCCGATCCGTTCGTGGACGTTAACTTCGTCGGCGCTGTGCTCCTCAAAGCGAGGAAGTGTGAGTTGTTGCTGCGCGACGAGTCGGCGAGCGAAGTCCGCGTCCACTACCAGGAGTTCAAAACCGACGTCACTGCTCTATTCCTCGCCGTTCATGCTGGAAACGTCGCTTTGGTGAAGAAATTACTGAGCATTGGTGCTGATGTAAATCAGAAACTCTTCAGGGGCTTCGCGACAACAGCAGCAGTGAGAGAGGGCCACCTTGAGATTCTAGAGATCCTACTGAAAGCTGGAGCATCTCAACCAGCCTGTGAGGAAGCTCTATTGGAGGCAAGTTGTCATGGACATGCTAGGCTTGTTGACCGGCTTATGGCCTCTGATTTGATTCGGCCCCATATTGCCGTGCATGCTATTGTCACAGCCAGCTGCAGGGGGTTTGCTGATGTGGTGGACACTCTCATGAAGTGTGGTGTGGATGCAAGTGCCGCTGATAGGATGCTGCTTCAATCGTCTAAGCCAGCTCTTCACACTAATGTTGACTGTAGTGCACTTGTGGCTGCAGTTGTGAGTAGGCAGGTCCCAATCATACGTTTGCTGCTACAGGCCGGTGTGAGGACGGATGTCAATGTGAGGTTGGGAGCATGGTCATGGGACCCAGCTACGGGGGAAGAGTTTCGGGTTGGGGCAGGGTTGGCGGAGCCATATCCCATTACCTGGTGTGCAGTGGAGTATTTTGAAGCAAGTGGTTCTGTCTTGCACATGCTCCTCCAAGGCCTTTCTCCGGATACCCCTCATTGTGGAAGAACTCTCCTGCACCACGCTATTCTATGCGGTAATGCAGGAGCTGTCCGTGTGCTCTTAAGTTGTGGTGCCAATGTAGAATCACCCGTTAAAGCAACTGGTGGTACCAGGTTTAATCCGATACACATGGCTGCCCGCCTTGGTTTGCCAACAATCGTTCAATGTTTAACTGAATCTGGATGTGATATGAACTCCAAGACTGATTCTGGCGAGACAGCTGTAATGATCTGCACAAAATACAAGCATGAAGAATGTCTCAGAGTATTGGCGGCTGCTGGTGCTGATTTTGGCTTGGTTAATGCGGCTGGTCAGTCTGTTAGTTCGATTGCCAGGGCAGCTAGGTGGTCACTTGGTTTTCAGCAGGCGTTGATGTGTATAATGAGTGACGGAAAGATGCCCAAATCCAGCAACTTTTCTGTTTTCTCTCCCCTGATGTTTGCAGCTCAAGCCGGCGATATTGAGGCCTTGAAAGCCGTAGTTGGTTCAGGAGAATTTGACATCGATTATCAAGATGATAAAGGTTTCACGGCCGTTATGATCACTGCTTTGAAGGGGCACGTTGAAGCATTCAGGTTTCTGGTTTATGCAGGGGCTGATGTAAAACTGTGTAACAAGTCTGGTGAGACTGCAATTACTCTATCAGAAATAAGCCAAAACCGTGACCTATTTGAGAAGGTAATGCTCGAATATGCACTAGAAAAGGGCAATCGTTACGCTGGAGGGTTCTACGCTCTGCATTGTGCAGCACGCCGTGGGGATGTGGATGCAGTCAAGTTGTTAACAAGTAGGGGATATGATGTGAATGTCCCTGACGGAGATGGTTACACTCCGCTCATGTTAGCGGCTAGGGAAGGTTACAGTCTCATGTGTGAACTCTTGATATCTCACGGGGCAAACTTGGATGTCAAGAATGCCAAAGGCGAAACACCGCTTTTGCTTGCGAGGAAAAGCGGCGGTGGTTTGAAAAATGAGGCAGAGCGGGTGATACTAGATGAGCTTGCTCGCAAGCTGGTGCTAGGTGGGGCGCGTGTCTGGAAGCATACGAAGGGAGGGAAAGGCAGCCCTCATGGGAAAGAGATGAGGATGGTGGGAACTGTAGGGGTGCTCTGCTGGGGGAAATCAAACCGGAGAAATGTGATATGCCGGGAGGCAGAGGCGGGGCCTAGTCCAGCCTTTAGGAGGAATCGGAGGAGCAAGAGCGATGCAGATGAACCCGGAGTGTTTAGGGTGGTGACTACCAAGAACAAGGAGGTGCATTTTTCGTGTGAAGGCGGGGTTGAAAGCGCCGAGCTTTGGGTGAGGGGTATAAAGCTTGTGACCAAGGAGGCTCTTTCAGTGATCGGAGCGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000278 GO:0000281 GO:0000902 GO:0000904 GO:0000910 GO:0001508 GO:0002028 GO:0003008 GO:0003254 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005794 GO:0005829 GO:0005856 GO:0005886 GO:0005911 GO:0006810 GO:0006888 GO:0006892 GO:0006893 GO:0006996 GO:0007009 GO:0007010 GO:0007016 GO:0007049 GO:0007154 GO:0007275 GO:0007399 GO:0007409 GO:0007528 GO:0008092 GO:0008104 GO:0008150 GO:0009653 GO:0009893 GO:0009986 GO:0009987 GO:0010035 GO:0010038 GO:0010256 GO:0010468 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010649 GO:0010650 GO:0010765 GO:0010959 GO:0010960 GO:0012505 GO:0014704 GO:0014731 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0016328 GO:0016528 GO:0016529 GO:0019222 GO:0019226 GO:0019228 GO:0022008 GO:0022402 GO:0022407 GO:0022409 GO:0022607 GO:0022898 GO:0023052 GO:0030016 GO:0030017 GO:0030018 GO:0030030 GO:0030054 GO:0030154 GO:0030155 GO:0030182 GO:0030315 GO:0030424 GO:0030425 GO:0030507 GO:0030674 GO:0031175 GO:0031594 GO:0031674 GO:0032026 GO:0032386 GO:0032388 GO:0032409 GO:0032410 GO:0032411 GO:0032412 GO:0032413 GO:0032414 GO:0032501 GO:0032502 GO:0032507 GO:0032879 GO:0032880 GO:0032989 GO:0032990 GO:0033036 GO:0033157 GO:0033267 GO:0033268 GO:0034110 GO:0034112 GO:0034613 GO:0034762 GO:0034763 GO:0034764 GO:0034765 GO:0034766 GO:0034767 GO:0035637 GO:0036477 GO:0042221 GO:0042383 GO:0042391 GO:0042592 GO:0042886 GO:0042995 GO:0043001 GO:0043005 GO:0043034 GO:0043194 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043266 GO:0043267 GO:0043269 GO:0043270 GO:0043271 GO:0043292 GO:0044085 GO:0044091 GO:0044092 GO:0044093 GO:0044291 GO:0044304 GO:0044325 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044449 GO:0044456 GO:0044459 GO:0044463 GO:0044464 GO:0045184 GO:0045185 GO:0045202 GO:0045211 GO:0045296 GO:0045760 GO:0045785 GO:0045838 GO:0046907 GO:0048193 GO:0048468 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048812 GO:0048856 GO:0048858 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050808 GO:0050839 GO:0050877 GO:0050896 GO:0051049 GO:0051050 GO:0051051 GO:0051179 GO:0051222 GO:0051223 GO:0051234 GO:0051235 GO:0051301 GO:0051641 GO:0051649 GO:0051651 GO:0051716 GO:0055065 GO:0055080 GO:0060090 GO:0060255 GO:0060341 GO:0061024 GO:0061564 GO:0061640 GO:0061951 GO:0065007 GO:0065008 GO:0065009 GO:0070201 GO:0070727 GO:0070887 GO:0071241 GO:0071248 GO:0071286 GO:0071702 GO:0071705 GO:0071709 GO:0071840 GO:0071944 GO:0072507 GO:0072657 GO:0072658 GO:0072659 GO:0072660 GO:0090087 GO:0090150 GO:0090313 GO:0090314 GO:0090316 GO:0097060 GO:0097447 GO:0097458 GO:0098590 GO:0098771 GO:0098794 GO:0098876 GO:0098900 GO:0098901 GO:0098902 GO:0099080 GO:0099081 GO:0099512 GO:0120025 GO:0120036 GO:0120038 GO:0120039 GO:1900825 GO:1900827 GO:1901016 GO:1901017 GO:1901379 GO:1901380 GO:1902259 GO:1902260 GO:1902305 GO:1902307 GO:1903047 GO:1903533 GO:1903817 GO:1903827 GO:1903829 GO:1904062 GO:1904063 GO:1904064 GO:1904181 GO:1904951 GO:1905475 GO:1905477 GO:1990778 GO:2000649 GO:2000651 GO:2001257 GO:2001258 GO:2001259
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3

Protein Analysis

754

Amino Acids

80.8

Weight (kDa)

7.25

Isoelectric Point (pI)

32.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 58 - 136 2e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 286 - 369 3.1e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 341 - 400 1.1e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 447 - 527 3.4e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 548 - 621 6.6e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 548 - 597 2.6e-08 Ankyrin repeats (many copies)
Ank_5 PF13857 569 - 616 1.9e-06 Ankyrin repeats (many copies)
Ank PF00023 576 - 607 1e-07 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 240
Acc36I ACCTGC 1 cut(s) 673
Acc65I GGTACC 1 cut(s) 1013
AccB1I GGYRCC 2 cut(s) 977, 1013
AccB7I CCANNNNNTGG 1 cut(s) 822
AccBSI CCGCTC 2 cut(s) 1741, 1888
AccII CGCG 4 cut(s) 186, 210, 341, 1936
AccIII TCCGGA 1 cut(s) 889
AclWI GGATC 2 cut(s) 110, 379
AcoI YGGCCR 1 cut(s) 1437
AcsI RAATTY 1 cut(s) 1400
AcuI CTGAAG 1 cut(s) 313
AcyI GRCGYC 1 cut(s) 237
AdeI CACNNNGTG 1 cut(s) 1661
AfaI GTAC 1 cut(s) 1015
AflII CTTAAG 1 cut(s) 967
AgsI TTSAA 9 cut(s) 229, 614, 845, 1070, 1378, 1459, 1472, 1873, 2189
AjnI CCWGG 4 cut(s) 219, 821, 1016, 1238
Alw21I GWGCWC 4 cut(s) 153, 658, 966, 2016
Alw26I GTCTC 4 cut(s) 1115, 1169, 1526, 1775
Alw44I GTGCAC 1 cut(s) 654
AlwI GGATC 2 cut(s) 110, 379
AlwNI CAGNNNCTG 2 cut(s) 953, 1502
Aor13HI TCCGGA 1 cut(s) 889
AoxI GGCC 9 cut(s) 42, 364, 480, 497, 711, 880, 1371, 1437, 2069
ApaLI GTGCAC 1 cut(s) 654
ApoI RAATTY 1 cut(s) 1400
ArsI GACNNNNNNTTYG 6 cut(s) 119, 151, 195, 222, 227, 254
Asp700I GAANNNNTTC 2 cut(s) 326, 780
Asp718I GGTACC 1 cut(s) 1013
AspLEI GCGC 5 cut(s) 100, 146, 186, 1936, 2195
AspS9I GGNCC 5 cut(s) 364, 498, 685, 760, 2069
AsuC2I CCSGG 3 cut(s) 29, 2054, 2124
AsuHPI GGTGA 7 cut(s) 95, 295, 984, 1541, 1903, 2152, 2218
AvaII GGWCC 2 cut(s) 685, 760
BaeGI GKGCMC 2 cut(s) 658, 1467
BanI GGYRCC 2 cut(s) 977, 1013
Bbv12I GWGCWC 4 cut(s) 153, 658, 966, 2016
BccI CCATC 2 cut(s) 1721, 1987
BceAI ACGGC 6 cut(s) 242, 494, 1367, 1424, 1452, 1644
BcgI CGANNNNNNTGC 2 cut(s) 224, 258
BciT130I CCWGG 4 cut(s) 221, 823, 1018, 1240
BciVI GTATCC 1 cut(s) 886
BclI TGATCA 1 cut(s) 1446
BcnI CCSGG 3 cut(s) 29, 2054, 2124
BcoDI GTCTC 4 cut(s) 1115, 1169, 1526, 1775
BfaI CTAG 8 cut(s) 380, 459, 1248, 1604, 1755, 1898, 1925, 2073
BfmI CTRYAG 5 cut(s) 535, 649, 666, 707, 2004
BfoI RGCGCY 3 cut(s) 101, 147, 2196
BfrI CTTAAG 1 cut(s) 967
BfuAI ACCTGC 1 cut(s) 673
BfuI GTATCC 1 cut(s) 886
Bme1390I CCNGG 7 cut(s) 29, 221, 823, 1018, 1240, 2054, 2124
Bme18I GGWCC 2 cut(s) 685, 760
BmgT120I GGNCC 5 cut(s) 364, 498, 685, 760, 2069
BmiI GGNNCC 8 cut(s) 500, 687, 761, 762, 807, 979, 1015, 2070
BmrFI CCNGG 7 cut(s) 29, 221, 823, 1018, 1240, 2054, 2124
BmsI GCATC 7 cut(s) 117, 413, 574, 594, 1293, 1663, 2101
BoxI GACNNNNGTC 1 cut(s) 728
BpmI CTGGAG 3 cut(s) 122, 420, 1647
BpuEI CTTGAG 2 cut(s) 392, 1338
BpuMI CCSGG 3 cut(s) 29, 2054, 2124
Bsa29I ATCGAT 1 cut(s) 1410
BsaBI GATNNNNATC 1 cut(s) 315
BsaHI GRCGYC 1 cut(s) 237
BsaJI CCNNGG 6 cut(s) 45, 877, 1048, 1239, 1660, 2229
BsaWI WCCGGW 3 cut(s) 80, 889, 2034
Bse118I RCCGGY 3 cut(s) 471, 713, 1358
Bse1I ACTGG 1 cut(s) 1012
Bse8I GATNNNNATC 1 cut(s) 315
BseAI TCCGGA 1 cut(s) 889
BseBI CCWGG 4 cut(s) 221, 823, 1018, 1240
BseCI ATCGAT 1 cut(s) 1410
BseDI CCNNGG 6 cut(s) 45, 877, 1048, 1239, 1660, 2229
BseGI GGATG 8 cut(s) 589, 609, 733, 1094, 1672, 1678, 1816, 1998
BseJI GATNNNNATC 1 cut(s) 315
BseMII CTCAG 2 cut(s) 287, 1180
BseNI ACTGG 1 cut(s) 1012
BseRI GAGGAG 3 cut(s) 143, 863, 2113
BseSI GKGCMC 2 cut(s) 658, 1467
BseX3I CGGCCG 1 cut(s) 1437
BseYI CCCAGC 2 cut(s) 763, 2019
BsgI GTGCAG 4 cut(s) 849, 905, 1123, 1671
Bsh1236I CGCG 4 cut(s) 186, 210, 341, 1936
Bsh1285I CGRYCG 1 cut(s) 1440
BshFI GGCC 9 cut(s) 44, 366, 482, 499, 713, 882, 1373, 1439, 2071
BshNI GGYRCC 2 cut(s) 977, 1013
BshVI ATCGAT 1 cut(s) 1410
BsiEI CGRYCG 1 cut(s) 1440
BsiHKAI GWGCWC 4 cut(s) 153, 658, 966, 2016
BslFI GGGAC 3 cut(s) 671, 773, 1700
BsmAI GTCTC 4 cut(s) 1115, 1169, 1526, 1775
BsmFI GGGAC 3 cut(s) 671, 773, 1700
BsmI GAATGC 2 cut(s) 1475, 1825
BsnI GGCC 9 cut(s) 44, 366, 482, 499, 713, 882, 1373, 1439, 2071
Bsp1286I GDGCHC 5 cut(s) 153, 658, 966, 1467, 2016
Bsp13I TCCGGA 1 cut(s) 889
Bsp143I GATC 6 cut(s) 85, 115, 384, 1134, 1446, 2247
Bsp68I TCGCGA 1 cut(s) 341
BspANI GGCC 9 cut(s) 44, 366, 482, 499, 713, 882, 1373, 1439, 2071
BspCNI CTCAG 2 cut(s) 288, 1179
BspDI ATCGAT 1 cut(s) 1410
BspEI TCCGGA 1 cut(s) 889
BspFNI CGCG 4 cut(s) 186, 210, 341, 1936
BspHI TCATGA 2 cut(s) 567, 2258
BspLI GGNNCC 8 cut(s) 500, 687, 761, 762, 807, 979, 1015, 2070
BspMAI CTGCAG 2 cut(s) 539, 670
BspMI ACCTGC 1 cut(s) 673
BspPI GGATC 2 cut(s) 110, 379
BspQI GCTCTTC 1 cut(s) 636
BspT107I GGYRCC 2 cut(s) 977, 1013
BspTI CTTAAG 1 cut(s) 967
BsrBI CCGCTC 2 cut(s) 1741, 1888
BsrFI RCCGGY 3 cut(s) 471, 713, 1358
BsrI ACTGG 1 cut(s) 1012
BssAI RCCGGY 3 cut(s) 471, 713, 1358
BssECI CCNNGG 6 cut(s) 45, 877, 1048, 1239, 1660, 2229
BssMI GATC 6 cut(s) 85, 115, 384, 1134, 1446, 2247
BssNI GRCGYC 1 cut(s) 237
BssT1I CCWWGG 3 cut(s) 877, 1048, 2229
Bst2UI CCWGG 4 cut(s) 221, 823, 1018, 1240
Bst4CI ACNGT 5 cut(s) 650, 1515, 1568, 1769, 2005
Bst6I CTCTTC 3 cut(s) 332, 636, 771
BstACI GRCGYC 1 cut(s) 237
BstAFI CTTAAG 1 cut(s) 967
BstAPI GCANNNNNTGC 1 cut(s) 61
BstDEI CTNAG 3 cut(s) 296, 621, 1166
BstDSI CCRYGG 1 cut(s) 1660
BstENI CCTNNNNNAGG 1 cut(s) 2086
BstF5I GGATG 8 cut(s) 589, 609, 733, 1094, 1672, 1678, 1816, 1998
BstFNI CGCG 4 cut(s) 186, 210, 341, 1936
BstH2I RGCGCY 3 cut(s) 101, 147, 2196
BstHHI GCGC 5 cut(s) 100, 146, 186, 1936, 2195
BstKTI GATC 6 cut(s) 88, 118, 387, 1137, 1449, 2250
BstMAI GTCTC 4 cut(s) 1115, 1169, 1526, 1775
BstMBI GATC 6 cut(s) 85, 115, 384, 1134, 1446, 2247
BstMCI CGRYCG 1 cut(s) 1440
BstNI CCWGG 4 cut(s) 221, 823, 1018, 1240
BstNSI RCATGY 3 cut(s) 458, 518, 871
BstPAI GACNNNNGTC 1 cut(s) 728
BstSCI CCNGG 7 cut(s) 27, 219, 821, 1016, 1238, 2052, 2122
BstSFI CTRYAG 5 cut(s) 535, 649, 666, 707, 2004
BstSLI GKGCMC 2 cut(s) 658, 1467
BstUI CGCG 4 cut(s) 186, 210, 341, 1936
BstX2I RGATCY 1 cut(s) 384
BstYI RGATCY 1 cut(s) 384
BstZI CGGCCG 1 cut(s) 1437
Bsu15I ATCGAT 1 cut(s) 1410
BsuI GTATCC 1 cut(s) 886
BsuRI GGCC 9 cut(s) 44, 366, 482, 499, 713, 882, 1373, 1439, 2071
BsuTUI ATCGAT 1 cut(s) 1410
BtgI CCRYGG 1 cut(s) 1660
BtgZI GCGATG 2 cut(s) 94, 2124
BtsCI GGATG 8 cut(s) 589, 609, 733, 1094, 1672, 1678, 1816, 1998
BtsI GCAGTG 4 cut(s) 240, 360, 837, 1449
BtsIMutI CAGTG 5 cut(s) 240, 360, 837, 1449, 2250
BtuMI TCGCGA 1 cut(s) 341
BveI ACCTGC 1 cut(s) 673
CaiI CAGNNNCTG 2 cut(s) 953, 1502
CciI TCATGA 2 cut(s) 567, 2258
CfoI GCGC 5 cut(s) 100, 146, 186, 1936, 2195
Cfr10I RCCGGY 3 cut(s) 471, 713, 1358
Cfr13I GGNCC 5 cut(s) 364, 498, 685, 760, 2069
ClaI ATCGAT 1 cut(s) 1410
CseI GACGC 2 cut(s) 199, 2244
CsiI ACCWGGT 2 cut(s) 821, 1016
Csp6I GTAC 1 cut(s) 1014
CviQI GTAC 1 cut(s) 1014
DdeI CTNAG 3 cut(s) 296, 621, 1166
DpnI GATC 6 cut(s) 87, 117, 386, 1136, 1448, 2249
DpnII GATC 6 cut(s) 85, 115, 384, 1134, 1446, 2247
DraIII CACNNNGTG 1 cut(s) 1661
EaeI YGGCCR 1 cut(s) 1437
EagI CGGCCG 1 cut(s) 1437
Eam1104I CTCTTC 3 cut(s) 332, 636, 771
EarI CTCTTC 3 cut(s) 332, 636, 771
EciI GGCGGA 1 cut(s) 818
EclXI CGGCCG 1 cut(s) 1437
Eco130I CCWWGG 3 cut(s) 877, 1048, 2229
Eco147I AGGCCT 2 cut(s) 882, 1373
Eco32I GATATC 1 cut(s) 1791
Eco47I GGWCC 2 cut(s) 685, 760
Eco52I CGGCCG 1 cut(s) 1437
Eco57I CTGAAG 1 cut(s) 313
EcoNI CCTNNNNNAGG 1 cut(s) 2086
EcoO109I RGGNCCY 3 cut(s) 685, 760, 2069
EcoRII CCWGG 4 cut(s) 219, 821, 1016, 1238
EcoRV GATATC 1 cut(s) 1791
EcoT14I CCWWGG 3 cut(s) 877, 1048, 2229
ErhI CCWWGG 3 cut(s) 877, 1048, 2229
FaqI GGGAC 3 cut(s) 671, 773, 1700
FauI CCCGC 4 cut(s) 1052, 1881, 2059, 2174
FbaI TGATCA 1 cut(s) 1446
FokI GGATG 8 cut(s) 596, 616, 740, 1101, 1679, 1685, 1823, 2005
FspBI CTAG 8 cut(s) 380, 459, 1248, 1604, 1755, 1898, 1925, 2073
GlaI GCGC 5 cut(s) 99, 145, 185, 1935, 2194
GsaI CCCAGC 2 cut(s) 767, 2023
GsuI CTGGAG 3 cut(s) 122, 420, 1647
HaeII RGCGCY 3 cut(s) 101, 147, 2196
HaeIII GGCC 9 cut(s) 44, 366, 482, 499, 713, 882, 1373, 1439, 2071
HgaI GACGC 2 cut(s) 199, 2244
HhaI GCGC 5 cut(s) 100, 146, 186, 1936, 2195
Hin1I GRCGYC 1 cut(s) 237
Hin6I GCGC 5 cut(s) 98, 144, 184, 1934, 2193
HinP1I GCGC 5 cut(s) 98, 144, 184, 1934, 2193
HincII GTYRAC 4 cut(s) 133, 469, 646, 1689
HindII GTYRAC 4 cut(s) 133, 469, 646, 1689
HindIII AAGCTT 1 cut(s) 2219
HinfI GANTC 7 cut(s) 191, 376, 493, 989, 1082, 1112, 2092
HpaI GTTAAC 2 cut(s) 133, 1689
HphI GGTGA 7 cut(s) 95, 295, 984, 1541, 1903, 2152, 2218
Hpy188I TCNGA 8 cut(s) 90, 321, 487, 1029, 1169, 1552, 2097, 2252
Hpy99I CGWCG 4 cut(s) 143, 191, 239, 278
HpyAV CCTTC 5 cut(s) 1453, 1576, 1754, 1948, 2171
HpyCH4III ACNGT 5 cut(s) 650, 1515, 1568, 1769, 2005
HpyCH4IV ACGT 5 cut(s) 129, 237, 273, 697, 1467
HpyF3I CTNAG 3 cut(s) 296, 621, 1166
HpySE526I ACGT 5 cut(s) 129, 237, 273, 697, 1467
Hsp92I GRCGYC 1 cut(s) 237
HspAI GCGC 5 cut(s) 98, 144, 184, 1934, 2193
KflI GGGWCCC 1 cut(s) 760
Kpn2I TCCGGA 1 cut(s) 889
KpnI GGTACC 1 cut(s) 1017
KroI GCCGGC 1 cut(s) 1358
KroNI GCCGGC 1 cut(s) 1360
Ksp22I TGATCA 1 cut(s) 1446
KspAI GTTAAC 2 cut(s) 133, 1689
Kzo9I GATC 6 cut(s) 85, 115, 384, 1134, 1446, 2247
LguI GCTCTTC 1 cut(s) 636
LmnI GCTCC 8 cut(s) 156, 401, 746, 805, 876, 950, 2100, 2252
LweI GCATC 7 cut(s) 117, 413, 574, 594, 1293, 1663, 2101
MabI ACCWGGT 2 cut(s) 821, 1016
MaeI CTAG 8 cut(s) 380, 459, 1248, 1604, 1755, 1898, 1925, 2073
MaeII ACGT 5 cut(s) 129, 237, 273, 697, 1467
MalI GATC 6 cut(s) 87, 117, 386, 1136, 1448, 2249
MbiI CCGCTC 2 cut(s) 1741, 1888
MboI GATC 6 cut(s) 85, 115, 384, 1134, 1446, 2247
MboII GAAGA 6 cut(s) 298, 319, 623, 788, 921, 1169
MflI RGATCY 1 cut(s) 384
MhlI GDGCHC 5 cut(s) 153, 658, 966, 1467, 2016
MluCI AATT 3 cut(s) 290, 1400, 1539
MlyI GAGTC 1 cut(s) 200
MroI TCCGGA 1 cut(s) 889
MroNI GCCGGC 1 cut(s) 1358
MroXI GAANNNNTTC 2 cut(s) 326, 780
MseI TTAA 7 cut(s) 132, 968, 999, 1023, 1076, 1206, 1688
MslI CAYNNNNRTG 4 cut(s) 453, 566, 639, 754
MspA1I CMGCKG 3 cut(s) 534, 596, 1127
MspCI CTTAAG 1 cut(s) 967
MspR9I CCNGG 7 cut(s) 29, 221, 823, 1018, 1240, 2054, 2124
Mva1269I GAATGC 2 cut(s) 1475, 1825
MvaI CCWGG 4 cut(s) 221, 823, 1018, 1240
MvnI CGCG 4 cut(s) 186, 210, 341, 1936
NaeI GCCGGC 1 cut(s) 1360
NciI CCSGG 3 cut(s) 29, 2054, 2124
NdeII GATC 6 cut(s) 85, 115, 384, 1134, 1446, 2247
NgoMIV GCCGGC 1 cut(s) 1358
NlaIV GGNNCC 8 cut(s) 500, 687, 761, 762, 807, 979, 1015, 2070
NmeAIII GCCGAG 2 cut(s) 70, 2221
NmuCI GTSAC 7 cut(s) 238, 523, 1254, 1292, 1568, 2140, 2224
NruI TCGCGA 1 cut(s) 341
NspI RCATGY 3 cut(s) 458, 518, 871
PaeI GCATGC 1 cut(s) 518
PagI TCATGA 2 cut(s) 567, 2258
PceI AGGCCT 2 cut(s) 882, 1373
PciSI GCTCTTC 1 cut(s) 636
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PctI GAATGC 2 cut(s) 1475, 1825
PdiI GCCGGC 1 cut(s) 1360
PdmI GAANNNNTTC 2 cut(s) 326, 780
PfeI GAWTC 6 cut(s) 376, 493, 989, 1082, 1112, 2092
PflMI CCANNNNNTGG 1 cut(s) 822
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
PpuMI RGGWCCY 2 cut(s) 685, 760
PshAI GACNNNNGTC 1 cut(s) 728
Psp5II RGGWCCY 2 cut(s) 685, 760
Psp6I CCWGG 4 cut(s) 219, 821, 1016, 1238
PspFI CCCAGC 2 cut(s) 763, 2019
PspGI CCWGG 4 cut(s) 219, 821, 1016, 1238
PspN4I GGNNCC 8 cut(s) 500, 687, 761, 762, 807, 979, 1015, 2070
PspPI GGNCC 5 cut(s) 364, 498, 685, 760, 2069
PspPPI RGGWCCY 2 cut(s) 685, 760
PstI CTGCAG 2 cut(s) 539, 670
PstNI CAGNNNCTG 2 cut(s) 953, 1502
PsuI RGATCY 1 cut(s) 384
PvuII CAGCTG 2 cut(s) 534, 1127
RruI TCGCGA 1 cut(s) 341
RsaI GTAC 1 cut(s) 1015
RsaNI GTAC 1 cut(s) 1014
RseI CAYNNNNRTG 4 cut(s) 453, 566, 639, 754
SapI GCTCTTC 1 cut(s) 636
SaqAI TTAA 7 cut(s) 132, 968, 999, 1023, 1076, 1206, 1688
Sau3AI GATC 6 cut(s) 85, 115, 384, 1134, 1446, 2247
Sau96I GGNCC 5 cut(s) 364, 498, 685, 760, 2069
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 7 cut(s) 29, 221, 823, 1018, 1240, 2054, 2124
SduI GDGCHC 5 cut(s) 153, 658, 966, 1467, 2016
SexAI ACCWGGT 2 cut(s) 821, 1016
SfaNI GCATC 7 cut(s) 117, 413, 574, 594, 1293, 1663, 2101
SfcI CTRYAG 5 cut(s) 535, 649, 666, 707, 2004
SinI GGWCC 2 cut(s) 685, 760
SmiMI CAYNNNNRTG 4 cut(s) 453, 566, 639, 754
SmlI CTYRAG 3 cut(s) 371, 967, 1353
SmoI CTYRAG 3 cut(s) 371, 967, 1353
SphI GCATGC 1 cut(s) 518
Sse9I AATT 3 cut(s) 290, 1400, 1539
SseBI AGGCCT 2 cut(s) 882, 1373
SspMI CTAG 8 cut(s) 380, 459, 1248, 1604, 1755, 1898, 1925, 2073
StuI AGGCCT 2 cut(s) 882, 1373
StyD4I CCNGG 7 cut(s) 27, 219, 821, 1016, 1238, 2052, 2122
StyI CCWWGG 3 cut(s) 877, 1048, 2229
TaaI ACNGT 5 cut(s) 650, 1515, 1568, 1769, 2005
TaiI ACGT 5 cut(s) 132, 240, 276, 700, 1470
TaqI TCGA 4 cut(s) 66, 1232, 1410, 1593
TasI AATT 3 cut(s) 290, 1400, 1539
TauI GCSGC 5 cut(s) 596, 1181, 1214, 1754, 1863
TfiI GAWTC 6 cut(s) 376, 493, 989, 1082, 1112, 2092
Tru1I TTAA 7 cut(s) 132, 968, 999, 1023, 1076, 1206, 1688
Tru9I TTAA 7 cut(s) 132, 968, 999, 1023, 1076, 1206, 1688
TscAI CASTG 5 cut(s) 247, 360, 837, 1456, 2250
TseFI GTSAC 7 cut(s) 238, 523, 1254, 1292, 1568, 2140, 2224
Tsp45I GTSAC 7 cut(s) 238, 523, 1254, 1292, 1568, 2140, 2224
TspDTI ATGAA 5 cut(s) 251, 584, 1112, 1170, 2133
TspGWI ACGGA 5 cut(s) 108, 740, 947, 1311, 1737
TspRI CASTG 5 cut(s) 247, 360, 837, 1456, 2250
Van91I CCANNNNNTGG 1 cut(s) 822
Vha464I CTTAAG 1 cut(s) 967
VneI GTGCAC 1 cut(s) 654
VpaK11BI GGWCC 2 cut(s) 685, 760
XagI CCTNNNNNAGG 1 cut(s) 2086
XapI RAATTY 1 cut(s) 1400
XbaI TCTAGA 1 cut(s) 379
XceI RCATGY 3 cut(s) 458, 518, 871
XmnI GAANNNNTTC 2 cut(s) 326, 780
XspI CTAG 8 cut(s) 380, 459, 1248, 1604, 1755, 1898, 1925, 2073
ZraI GACGTC 1 cut(s) 238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.