Rroxscaffold_5G00372590

Ankyrin repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002610
Physical Location & Seq
Forward (+)
54006516 .. 54009992
3477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00372590.1

Sequence Viewer

Length: 2574 bp
ATGACGGTGTTCGCCGGAAAAGCGCACGTGTTTCCTATCAACTACGAAGCCGAGGTGTCGCAGCGGTTGGTCGACGCTGCTCACGACAATGATCCGAAGTCGGTGTGCGAGTGTCTGGAAGATCCTTTCGTCGACGTGAACTACGTCGGAACAGTGTGCTTGAAGAGTAGGAAGACAGAGATTGTGTTGAAAGGTGAGTCGGCTCACGAGGTTGGCGTCGAGTACGAGGAGTTCAAGACTCAGGTCACCGCCTTCTTCCTTGCAGCTCATTCCGGCAATTTGACGCTCGTCAGAAAACTACTGAGTTATGGAGCCAATGTGAATCTGAAACTGTTTCGAGGCTATGCGACGACAGCAGCAGTGAGGGAAGGACACGCACAGATACTGGAGGTTGTTATCAATGCTGGAGCATCTCAGAAGGCCTGCGAGGAGGCCTTGTTGGAGGCAAGCTACTTGGGCCGGGCTAGGCTGGCTGAGATGCTCATGGCATCCGAGTTGATCCGTCCTCAAGCTGCTGTACATGCTCTCGTTTCTGCTAGCTGCAGAGGGTTTGTTGATGTTATAGACATACTCATCAAGCGTGGGGTAAATATCGATGCAATTGACCGGACATTGCTTCAATCTTCGAAGCCATCACTATACACCAACGCCGACTGCAATGCTCTTGTTGCTGCCATTGTCAGCAGGCAGATTTCGGTTGTGAGATTATTGTTGCAGGCTGGAGCCAGAACAGATATCAAAGTGAGTCTTGGAGGCTGGTCCTGGGATGTAAGTACAGGAGAAGAGTTTCGGGTTGGTGCAGGACTAGCCGAGGCGTACAGCGTGACATGGTGTGCTGTGGAGTACTTTGAAGCCAGCGGTGCTATCTTGCAAATGCTCCTACAACATGTCTCCCCTAACATTGCTCATTTTGGGAGGACTCTCATTCACCATGCCATCTTGTGCAATAATGAAAGAGCAGTGGATGTGCTGTTGAGTTGTGGCGCAGATGTAGAGGTTCCGATTAAGACAACTACTTCAGAAACTGAGTATCCCATCCACATGGCTTCGCGGTTTGGATTAAGTAAGATTCTCCAACAACTGATCGATGGTGGCTGCAATGTCAACTCTCGAACGGACTCTAGAGAAACTCCTCTCATGATCTGTGCTAGATATAAACACCAGGAGTGTTTCAGAATTCTTGCTTCTAACGGTGCTGATTTTGGCCTGGTCAATTCTTGTGGTCAAAGTGCAAGTGTAATTGCAGAGTCAGCTAAGTGGAGTCTCGGCTTTAAAAGAGTAGTTCTAGACTTGATTCGAACTGGGAAGGTTGTTCAATCGAGTAATATGACAATCTTTTCTCCTCTATTGTTTGTGACTCAAGCAAATGATGTTGAGGCTTTGAAACAGCTGATCCATTGTGGTTGCAATATCAACTCACAAACAGAATCTGGAGAAACTGCACTAATGATCTGTGCTAGACATAAACACCAGGAATGCTTCAAAGTTCTTGCTTCAAATGGTGCCGATTTTGGCCTTGTCAACTCTTGCGGTCAGAGTGCAAGCTCAATTGCAGAGTCAGCCAAGTGGACTCCTATCTTCAAACAAGCTATCCTAGATGTGATTCGGGATGGGAAGGTTGTTCGATCAAGCAACACATCAATATGTTCTCCTCTAATGCTTGTGACTCAAGAAAACAGTGTTGAGGCTTTGAAACAACTGATTGGGAGGACAGACATCGATGTTGATGAGCAAGACGAAAATGGATACTCGGCTGCAATGATAGCTGCAGCAGATGGCAACTTGGAAGCTTTCAAATTGCTTATCTGTGCAGGAGCTGACATGAATCTGCAGAACAAACATGGTCAGACCCCATTGGACCTACTAGATACAAACCAAAACGGTGAAGAATTTGAAAAATTATTACTGAATAATGCAGTTCAAAAGGGCCCCAATAGTAGTTCCCTTGAGTTCTATGCTCTTCATCTTGCAGCGCAACATGGAGACTTGGATTTGGTTCATACATTGATAAGTCAGGGGTATGATGTTAATGCTTTCGATGCTGAGGGATACACTCCATTGATGTTAGCAGCAAGAGGTGGCCATGGTAAGCTGTGTGAGCTTTTGATTTCTTTTGGGGCTAGATGTGATACTGTGAATGCAAGACATGAGACGGCACTGTCTCTTGCAAGGAAAACTGGATCTAAAAATGATGCAGAGAAGGCTATATTGAATGAGTTTGCACTAAAACTGGTGCTGGGTGGCAGTCATGTCAAGAAGCACACAAAATGTGGTAAAGGAGCTCCCCATAGTAAATTGCTGAGAATGGTTGGGGCAGCTGGGCTTTTAAGGTGGGGGAAGTCAAGTAAGAGAAATGTGATCTGCAAGATGGCGGAGGTCGGGCCTAGTGAGGCATTCAGATGGAACCGCAGGAGGAAGTTCGACTGTGATGAACCCGGGATGTTCCATGTGGTGACTACAAAAAACAAGGAGCTGCATTTTGTGTGTGAAGGTGGGAATGAAATGGCAGAGTTGTGGGTGAGAGGGATCAAGCTTGTGACAATGAAAGCTATCTTTGGCAAGAATAATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4

Protein Analysis

857

Amino Acids

93.22

Weight (kDa)

6.53

Isoelectric Point (pI)

31.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 341 - 402 2.3e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 452 - 505 2.5e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 552 - 638 5.3e-10 Ankyrin repeats (3 copies)
Ank_5 PF13857 572 - 621 2.2e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 654 - 730 3.3e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 654 - 703 1.3e-10 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2158
AccB1I GGYRCC 1 cut(s) 1502
AccI GTMKAC 2 cut(s) 72, 132
AccII CGCG 1 cut(s) 1051
AciI CCGC 7 cut(s) 64, 249, 858, 1051, 1530, 2372, 2407
AclWI GGATC 6 cut(s) 86, 116, 493, 1387, 2188, 2534
AcoI YGGCCR 1 cut(s) 2080
AcsI RAATTY 2 cut(s) 1176, 1889
AcuI CTGAAG 1 cut(s) 1002
AcvI CACGTG 1 cut(s) 28
AcyI GRCGYC 1 cut(s) 216
AfaI GTAC 5 cut(s) 224, 519, 775, 818, 845
AflIII ACRYGT 2 cut(s) 27, 886
AjiI CACGTC 1 cut(s) 136
AjnI CCWGG 4 cut(s) 761, 1161, 1206, 1470
Alw21I GWGCWC 1 cut(s) 2284
Alw26I GTCTC 5 cut(s) 895, 1268, 1977, 2144, 2166
AlwI GGATC 6 cut(s) 86, 116, 493, 1387, 2188, 2534
AlwNI CAGNNNCTG 4 cut(s) 385, 1025, 1430, 1817
Ama87I CYCGRG 1 cut(s) 2435
AoxI GGCC 8 cut(s) 420, 432, 457, 1204, 1513, 1927, 2080, 2381
ApaI GGGCCC 1 cut(s) 1931
ApoI RAATTY 2 cut(s) 1176, 1889
Asp700I GAANNNNTTC 1 cut(s) 786
AspLEI GCGC 3 cut(s) 25, 986, 1975
AspS9I GGNCC 6 cut(s) 457, 759, 1858, 1927, 1928, 2381
AsuC2I CCSGG 3 cut(s) 461, 2436, 2437
AsuHPI GGTGA 6 cut(s) 206, 238, 920, 1895, 2464, 2530
AsuII TTCGAA 2 cut(s) 626, 1297
AsuNHI GCTAGC 1 cut(s) 536
AvaI CYCGRG 1 cut(s) 2435
AvaII GGWCC 2 cut(s) 759, 1858
BaeGI GKGCMC 1 cut(s) 1931
BalI TGGCCA 1 cut(s) 2082
BanI GGYRCC 1 cut(s) 1502
BanII GRGCYC 2 cut(s) 1931, 2284
BauI CACGAG 1 cut(s) 206
BbrPI CACGTG 1 cut(s) 28
BbsI GAAGAC 1 cut(s) 179
Bbv12I GWGCWC 1 cut(s) 2284
BbvCI CCTCAGC 1 cut(s) 2043
BccI CCATC 8 cut(s) 640, 944, 1043, 1082, 1604, 1769, 2362, 2394
BceAI ACGGC 1 cut(s) 2169
BcgI CGANNNNNNTGC 2 cut(s) 641, 675
BciT130I CCWGG 4 cut(s) 763, 1163, 1208, 1472
BciVI GTATCC 3 cut(s) 1041, 1739, 2042
BcnI CCSGG 3 cut(s) 461, 2436, 2437
BcoDI GTCTC 5 cut(s) 895, 1268, 1977, 2144, 2166
BfmI CTRYAG 3 cut(s) 541, 1767, 1829
BfuI GTATCC 3 cut(s) 1041, 1739, 2042
BmcAI AGTACT 1 cut(s) 845
Bme1390I CCNGG 7 cut(s) 461, 763, 1163, 1208, 1472, 2436, 2437
Bme18I GGWCC 2 cut(s) 759, 1858
BmeT110I CYCGRG 1 cut(s) 2435
BmgBI CACGTC 1 cut(s) 136
BmgT120I GGNCC 6 cut(s) 457, 759, 1858, 1927, 1928, 2381
BmiI GGNNCC 7 cut(s) 313, 724, 999, 1504, 1929, 1930, 2405
BmrFI CCNGG 7 cut(s) 461, 763, 1163, 1208, 1472, 2436, 2437
BmrI ACTGGG 1 cut(s) 1311
BmsI GCATC 6 cut(s) 419, 468, 497, 586, 2029, 2182
BmtI GCTAGC 1 cut(s) 540
BmuI ACTGGG 1 cut(s) 1311
BoxI GACNNNNGTC 2 cut(s) 242, 287
BpiI GAAGAC 1 cut(s) 179
BpmI CTGGAG 4 cut(s) 407, 426, 741, 1452
Bpu10I CCTNAGC 1 cut(s) 2043
Bpu14I TTCGAA 2 cut(s) 626, 1297
BpuEI CTTGAG 4 cut(s) 492, 1344, 1653, 1967
BpuMI CCSGG 3 cut(s) 461, 2436, 2437
Bsa29I ATCGAT 3 cut(s) 594, 1086, 1719
BsaAI YACGTR 1 cut(s) 28
BsaHI GRCGYC 1 cut(s) 216
BsaJI CCNNGG 5 cut(s) 51, 762, 810, 2083, 2435
BsaWI WCCGGW 1 cut(s) 606
BsaXI ACNNNNNCTCC 4 cut(s) 303, 333, 422, 452
Bse1I ACTGG 4 cut(s) 390, 1306, 2182, 2235
Bse3DI GCAATG 5 cut(s) 611, 664, 900, 1105, 1764
BseBI CCWGG 4 cut(s) 763, 1163, 1208, 1472
BseCI ATCGAT 3 cut(s) 594, 1086, 1719
BseDI CCNNGG 5 cut(s) 51, 762, 810, 2083, 2435
BseGI GGATG 6 cut(s) 488, 772, 970, 1035, 1615, 2445
BseMI GCAATG 5 cut(s) 611, 664, 900, 1105, 1764
BseMII CTCAG 7 cut(s) 254, 293, 428, 465, 1017, 2034, 2291
BseNI ACTGG 4 cut(s) 390, 1306, 2182, 2235
BseRI GAGGAG 5 cut(s) 242, 443, 1122, 1332, 1641
BseSI GKGCMC 1 cut(s) 1931
BseYI CCCAGC 2 cut(s) 2236, 2318
BsgI GTGCAG 3 cut(s) 819, 1425, 1830
Bsh1236I CGCG 1 cut(s) 1051
BshFI GGCC 8 cut(s) 422, 434, 459, 1206, 1515, 1929, 2082, 2383
BshNI GGYRCC 1 cut(s) 1502
BshVI ATCGAT 3 cut(s) 594, 1086, 1719
BsiHKAI GWGCWC 1 cut(s) 2284
BsiHKCI CYCGRG 1 cut(s) 2435
BsiSI CCGG 5 cut(s) 15, 273, 460, 607, 2436
BsmAI GTCTC 5 cut(s) 895, 1268, 1977, 2144, 2166
BsmBI CGTCTC 1 cut(s) 2144
BsmI GAATGC 3 cut(s) 1481, 2143, 2393
BsnI GGCC 8 cut(s) 422, 434, 459, 1206, 1515, 1929, 2082, 2383
BsoBI CYCGRG 1 cut(s) 2435
Bsp119I TTCGAA 2 cut(s) 626, 1297
Bsp120I GGGCCC 1 cut(s) 1927
Bsp1286I GDGCHC 2 cut(s) 1931, 2284
Bsp1407I TGTACA 1 cut(s) 517
Bsp19I CCATGG 1 cut(s) 2083
BspACI CCGC 7 cut(s) 64, 249, 858, 1051, 1530, 2372, 2407
BspANI GGCC 8 cut(s) 422, 434, 459, 1206, 1515, 1929, 2082, 2383
BspCNI CTCAG 7 cut(s) 253, 294, 427, 466, 1018, 2035, 2292
BspDI ATCGAT 3 cut(s) 594, 1086, 1719
BspFNI CGCG 1 cut(s) 1051
BspHI TCATGA 1 cut(s) 1137
BspLI GGNNCC 7 cut(s) 313, 724, 999, 1504, 1929, 1930, 2405
BspMAI CTGCAG 3 cut(s) 545, 1771, 1833
BspOI GCTAGC 1 cut(s) 540
BspPI GGATC 6 cut(s) 86, 116, 493, 1387, 2188, 2534
BspQI GCTCTTC 1 cut(s) 1965
BspT104I TTCGAA 2 cut(s) 626, 1297
BspT107I GGYRCC 1 cut(s) 1502
BsrDI GCAATG 5 cut(s) 611, 664, 900, 1105, 1764
BsrGI TGTACA 1 cut(s) 517
BsrI ACTGG 4 cut(s) 390, 1306, 2182, 2235
BssECI CCNNGG 5 cut(s) 51, 762, 810, 2083, 2435
BssNI GRCGYC 1 cut(s) 216
BssSI CACGAG 1 cut(s) 206
BssT1I CCWWGG 1 cut(s) 2083
Bst2BI CACGAG 1 cut(s) 206
Bst2UI CCWGG 4 cut(s) 763, 1163, 1208, 1472
Bst4CI ACNGT 9 cut(s) 7, 154, 333, 1193, 1679, 1883, 2134, 2160, 2426
Bst6I CTCTTC 3 cut(s) 158, 777, 1965
BstACI GRCGYC 1 cut(s) 216
BstAUI TGTACA 1 cut(s) 517
BstBAI YACGTR 1 cut(s) 28
BstBI TTCGAA 2 cut(s) 626, 1297
BstC8I GCNNGC 8 cut(s) 424, 448, 471, 538, 686, 717, 856, 1543
BstDEI CTNAG 8 cut(s) 240, 302, 414, 474, 1026, 1254, 2043, 2300
BstDSI CCRYGG 1 cut(s) 2083
BstEII GGTNACC 1 cut(s) 244
BstF5I GGATG 6 cut(s) 488, 772, 970, 1035, 1615, 2445
BstFNI CGCG 1 cut(s) 1051
BstHHI GCGC 3 cut(s) 25, 986, 1975
BstMAI GTCTC 5 cut(s) 895, 1268, 1977, 2144, 2166
BstNI CCWGG 4 cut(s) 763, 1163, 1208, 1472
BstNSI RCATGY 2 cut(s) 524, 890
BstPAI GACNNNNGTC 2 cut(s) 242, 287
BstPI GGTNACC 1 cut(s) 244
BstSCI CCNGG 7 cut(s) 459, 761, 1161, 1206, 1470, 2434, 2435
BstSFI CTRYAG 3 cut(s) 541, 1767, 1829
BstSLI GKGCMC 1 cut(s) 1931
BstUI CGCG 1 cut(s) 1051
BstV2I GAAGAC 1 cut(s) 179
BstX2I RGATCY 2 cut(s) 121, 2180
BstXI CCANNNNNNTGG 1 cut(s) 1042
BstYI RGATCY 2 cut(s) 121, 2180
Bsu15I ATCGAT 3 cut(s) 594, 1086, 1719
BsuI GTATCC 3 cut(s) 1041, 1739, 2042
BsuRI GGCC 8 cut(s) 422, 434, 459, 1206, 1515, 1929, 2082, 2383
BsuTUI ATCGAT 3 cut(s) 594, 1086, 1719
BtgI CCRYGG 1 cut(s) 2083
BtrI CACGTC 1 cut(s) 136
BtsCI GGATG 6 cut(s) 488, 772, 970, 1035, 1615, 2445
BtsI GCAGTG 2 cut(s) 366, 966
BtsIMutI CAGTG 5 cut(s) 159, 366, 966, 1684, 2156
Cac8I GCNNGC 8 cut(s) 424, 448, 471, 538, 686, 717, 856, 1543
CaiI CAGNNNCTG 4 cut(s) 385, 1025, 1430, 1817
CciI TCATGA 1 cut(s) 1137
CfoI GCGC 3 cut(s) 25, 986, 1975
Cfr13I GGNCC 6 cut(s) 457, 759, 1858, 1927, 1928, 2381
Cfr9I CCCGGG 1 cut(s) 2435
ClaI ATCGAT 3 cut(s) 594, 1086, 1719
CseI GACGC 3 cut(s) 83, 205, 292
Csp6I GTAC 5 cut(s) 223, 518, 774, 817, 844
CspCI CAANNNNNGTGG 2 cut(s) 1201, 1236
CviQI GTAC 5 cut(s) 223, 518, 774, 817, 844
DdeI CTNAG 8 cut(s) 240, 302, 414, 474, 1026, 1254, 2043, 2300
DraI TTTAAA 1 cut(s) 1273
DrdI GACNNNNNNGTC 1 cut(s) 2158
DseDI GACNNNNNNGTC 1 cut(s) 2158
EaeI YGGCCR 1 cut(s) 2080
Eam1104I CTCTTC 3 cut(s) 158, 777, 1965
EarI CTCTTC 3 cut(s) 158, 777, 1965
EciI GGCGGA 1 cut(s) 2387
Ecl136II GAGCTC 1 cut(s) 2282
Eco130I CCWWGG 1 cut(s) 2083
Eco147I AGGCCT 2 cut(s) 422, 434
Eco24I GRGCYC 2 cut(s) 1931, 2284
Eco32I GATATC 1 cut(s) 736
Eco47I GGWCC 2 cut(s) 759, 1858
Eco53kI GAGCTC 1 cut(s) 2282
Eco57I CTGAAG 1 cut(s) 1002
Eco72I CACGTG 1 cut(s) 28
Eco88I CYCGRG 1 cut(s) 2435
Eco91I GGTNACC 1 cut(s) 244
EcoICRI GAGCTC 1 cut(s) 2282
EcoO109I RGGNCCY 2 cut(s) 1927, 1928
EcoO65I GGTNACC 1 cut(s) 244
EcoRI GAATTC 1 cut(s) 1176
EcoRII CCWGG 4 cut(s) 761, 1161, 1206, 1470
EcoRV GATATC 1 cut(s) 736
EcoT14I CCWWGG 1 cut(s) 2083
EcoT38I GRGCYC 2 cut(s) 1931, 2284
ErhI CCWWGG 1 cut(s) 2083
Esp3I CGTCTC 1 cut(s) 2144
FalI AAGNNNNNCTT 2 cut(s) 732, 764
FblI GTMKAC 2 cut(s) 72, 132
FokI GGATG 6 cut(s) 475, 779, 977, 1022, 1622, 2452
FriOI GRGCYC 2 cut(s) 1931, 2284
GlaI GCGC 3 cut(s) 24, 985, 1974
GsaI CCCAGC 2 cut(s) 2240, 2322
GsuI CTGGAG 4 cut(s) 407, 426, 741, 1452
HaeIII GGCC 8 cut(s) 422, 434, 459, 1206, 1515, 1929, 2082, 2383
HapII CCGG 5 cut(s) 15, 273, 460, 607, 2436
HgaI GACGC 3 cut(s) 83, 205, 292
HhaI GCGC 3 cut(s) 25, 986, 1975
Hin1I GRCGYC 1 cut(s) 216
Hin6I GCGC 3 cut(s) 23, 984, 1973
HinP1I GCGC 3 cut(s) 23, 984, 1973
HincII GTYRAC 4 cut(s) 73, 133, 1105, 1522
HindII GTYRAC 4 cut(s) 73, 133, 1105, 1522
HindIII AAGCTT 2 cut(s) 1788, 2531
HpaII CCGG 5 cut(s) 15, 273, 460, 607, 2436
HphI GGTGA 6 cut(s) 206, 238, 920, 1895, 2464, 2530
Hpy166II GTNNAC 6 cut(s) 73, 133, 139, 1105, 1522, 1569
Hpy8I GTNNAC 6 cut(s) 73, 133, 139, 1105, 1522, 1569
Hpy99I CGWCG 6 cut(s) 77, 134, 137, 149, 221, 352
HpyAV CCTTC 7 cut(s) 262, 362, 412, 1300, 1609, 2194, 2483
HpyCH4III ACNGT 9 cut(s) 7, 154, 333, 1193, 1679, 1883, 2134, 2160, 2426
HpyCH4IV ACGT 3 cut(s) 27, 135, 144
HpyF3I CTNAG 8 cut(s) 240, 302, 414, 474, 1026, 1254, 2043, 2300
HpySE526I ACGT 3 cut(s) 27, 135, 144
Hsp92I GRCGYC 1 cut(s) 216
HspAI GCGC 3 cut(s) 23, 984, 1973
LguI GCTCTTC 1 cut(s) 1965
LmnI GCTCC 8 cut(s) 311, 407, 722, 882, 1814, 2279, 2287, 2470
LweI GCATC 6 cut(s) 419, 468, 497, 586, 2029, 2182
MaeII ACGT 3 cut(s) 27, 135, 144
MaeIII GTNAC 6 cut(s) 244, 823, 1354, 1663, 2452, 2536
MboII GAAGA 9 cut(s) 131, 175, 184, 247, 615, 794, 1570, 1898, 1952
MfeI CAATTG 2 cut(s) 600, 1548
MflI RGATCY 2 cut(s) 121, 2180
MhlI GDGCHC 2 cut(s) 1931, 2284
MlsI TGGCCA 1 cut(s) 2082
MluNI TGGCCA 1 cut(s) 2082
MmeI TCCRAC 3 cut(s) 127, 420, 1099
Mox20I TGGCCA 1 cut(s) 2082
MroXI GAANNNNTTC 1 cut(s) 786
MscI TGGCCA 1 cut(s) 2082
MseI TTAA 5 cut(s) 1005, 1061, 1272, 2028, 2327
MslI CAYNNNNRTG 4 cut(s) 87, 1040, 1642, 2398
Msp20I TGGCCA 1 cut(s) 2082
MspA1I CMGCKG 4 cut(s) 64, 858, 1390, 2318
MspI CCGG 5 cut(s) 15, 273, 460, 607, 2436
MspR9I CCNGG 7 cut(s) 461, 763, 1163, 1208, 1472, 2436, 2437
MunI CAATTG 2 cut(s) 600, 1548
Mva1269I GAATGC 3 cut(s) 1481, 2143, 2393
MvaI CCWGG 4 cut(s) 763, 1163, 1208, 1472
MvnI CGCG 1 cut(s) 1051
NciI CCSGG 3 cut(s) 461, 2436, 2437
NcoI CCATGG 1 cut(s) 2083
NheI GCTAGC 1 cut(s) 536
NlaIV GGNNCC 7 cut(s) 313, 724, 999, 1504, 1929, 1930, 2405
NmeAIII GCCGAG 4 cut(s) 76, 835, 1245, 1730
NmuCI GTSAC 6 cut(s) 244, 823, 1354, 1663, 2452, 2536
NspI RCATGY 2 cut(s) 524, 890
NspV TTCGAA 2 cut(s) 626, 1297
PagI TCATGA 1 cut(s) 1137
PceI AGGCCT 2 cut(s) 422, 434
PciI ACATGT 1 cut(s) 886
PciSI GCTCTTC 1 cut(s) 1965
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 141, 213
PctI GAATGC 3 cut(s) 1481, 2143, 2393
PdmI GAANNNNTTC 1 cut(s) 786
PfeI GAWTC 6 cut(s) 322, 1069, 1294, 1427, 1603, 1825
PmaCI CACGTG 1 cut(s) 28
PmlI CACGTG 1 cut(s) 28
Ppu21I YACGTR 1 cut(s) 28
PscI ACATGT 1 cut(s) 886
PshAI GACNNNNGTC 2 cut(s) 242, 287
Psp124BI GAGCTC 1 cut(s) 2284
Psp6I CCWGG 4 cut(s) 761, 1161, 1206, 1470
PspCI CACGTG 1 cut(s) 28
PspEI GGTNACC 1 cut(s) 244
PspFI CCCAGC 2 cut(s) 2236, 2318
PspGI CCWGG 4 cut(s) 761, 1161, 1206, 1470
PspN4I GGNNCC 7 cut(s) 313, 724, 999, 1504, 1929, 1930, 2405
PspOMI GGGCCC 1 cut(s) 1927
PspPI GGNCC 6 cut(s) 457, 759, 1858, 1927, 1928, 2381
PsrI GAACNNNNNNTAC 2 cut(s) 215, 247
PstI CTGCAG 3 cut(s) 545, 1771, 1833
PstNI CAGNNNCTG 4 cut(s) 385, 1025, 1430, 1817
PsuI RGATCY 2 cut(s) 121, 2180
PvuII CAGCTG 2 cut(s) 1390, 2318
RsaI GTAC 5 cut(s) 224, 519, 775, 818, 845
RsaNI GTAC 5 cut(s) 223, 518, 774, 817, 844
RseI CAYNNNNRTG 4 cut(s) 87, 1040, 1642, 2398
SacI GAGCTC 1 cut(s) 2284
SalI GTCGAC 2 cut(s) 71, 131
SapI GCTCTTC 1 cut(s) 1965
SaqAI TTAA 5 cut(s) 1005, 1061, 1272, 2028, 2327
Sau96I GGNCC 6 cut(s) 457, 759, 1858, 1927, 1928, 2381
ScaI AGTACT 1 cut(s) 845
ScrFI CCNGG 7 cut(s) 461, 763, 1163, 1208, 1472, 2436, 2437
SduI GDGCHC 2 cut(s) 1931, 2284
SfaNI GCATC 6 cut(s) 419, 468, 497, 586, 2029, 2182
SfcI CTRYAG 3 cut(s) 541, 1767, 1829
SfuI TTCGAA 2 cut(s) 626, 1297
SgrDI CGTCGACG 1 cut(s) 131
SinI GGWCC 2 cut(s) 759, 1858
SmaI CCCGGG 1 cut(s) 2437
SmiMI CAYNNNNRTG 4 cut(s) 87, 1040, 1642, 2398
SmlI CTYRAG 4 cut(s) 507, 1359, 1668, 1946
SmoI CTYRAG 4 cut(s) 507, 1359, 1668, 1946
SseBI AGGCCT 2 cut(s) 422, 434
SsiI CCGC 7 cut(s) 64, 249, 858, 1051, 1530, 2372, 2407
SstI GAGCTC 1 cut(s) 2284
StuI AGGCCT 2 cut(s) 422, 434
StyD4I CCNGG 7 cut(s) 459, 761, 1161, 1206, 1470, 2434, 2435
StyI CCWWGG 1 cut(s) 2083
TaaI ACNGT 9 cut(s) 7, 154, 333, 1193, 1679, 1883, 2134, 2160, 2426
TaiI ACGT 3 cut(s) 30, 138, 147
TatI WGTACW 3 cut(s) 517, 773, 843
TfiI GAWTC 6 cut(s) 322, 1069, 1294, 1427, 1603, 1825
Tru1I TTAA 5 cut(s) 1005, 1061, 1272, 2028, 2327
Tru9I TTAA 5 cut(s) 1005, 1061, 1272, 2028, 2327
TscAI CASTG 5 cut(s) 159, 366, 966, 1684, 2163
TseFI GTSAC 6 cut(s) 244, 823, 1354, 1663, 2452, 2536
Tsp45I GTSAC 6 cut(s) 244, 823, 1354, 1663, 2452, 2536
TspDTI ATGAA 7 cut(s) 966, 1838, 1952, 1988, 2445, 2514, 2558
TspGWI ACGGA 2 cut(s) 491, 1130
TspMI CCCGGG 1 cut(s) 2435
TspRI CASTG 5 cut(s) 159, 366, 966, 1684, 2163
VpaK11BI GGWCC 2 cut(s) 759, 1858
XapI RAATTY 2 cut(s) 1176, 1889
XbaI TCTAGA 2 cut(s) 1121, 1285
XceI RCATGY 2 cut(s) 524, 890
XmaI CCCGGG 1 cut(s) 2435
XmiI GTMKAC 2 cut(s) 72, 132
XmnI GAANNNNTTC 1 cut(s) 786
ZrmI AGTACT 1 cut(s) 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.