FvH4_4g31430

CAAX protease self-immunity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004201
Physical Location & Seq
Reverse (-)
30568241 .. 30571645
3405 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31430.t3

Sequence Viewer

Length: 1020 bp
ATGGTGTATACCCTCTTCACATCTCTAGCTCAGTCTCCCCTTCCCACTTTCTCAAACTCCCCCAGCTTAAATTCCTCCAAAACCTTCCTCAAACGAACCCACACCCTTTTCCTCAATGCCCATACAACCCACCTCACACGCCTCAGAACCACCCGGCCGCCGCTTCATGTCTGCTTTTCCGGCGGCCCACGAAACCCAAAACCTGATTTCCAAGAAAAGGAAAATGAAGGGGGGTTGCAGATTTTGAAGAGGTGGGATGTGCCATGGGAGTGGCAAACAGTGTCATTGACATCACTGGCTTGTGGGCTGAGTTTTGTTTTGACAGGATTGGTGGAGGCAGCAGTTGTACCCTATTTAGGATTTAGCATTCAGGATTTGAGTTTGGATGAGAAGGCTGAGTTTCTCTTGGTTGCTCAAGGTGTTACGACTGCAGCTATACTTGGAGTTCTGTATACTGTTGCAAGCACTTTCCAACCGCTGCCTCAAGATATTTACCGCTATGAGTGGAGGGATCCTTTCAGTCTACAAAGAGGGTGGCTTTTGTGGGCTGTGGTTGGTCTTGTTGGTGCTGTAGCTGCTGTGGCGTTGACAGGGGCAGCCACATCCTTTTTCAGTGGGGAGCCCCCACAAAGAGAGAAGGACGCTCTAGTTAGCTTACTCCCGCTAATTGGATCATCAAGTGTTAGCACTGCTTGCTTGTTAGGCATCACTGGAGTTTTGGCTCCAATTCTTGAGGAAACTGTGTTCCGCGGGTTTTTCATGGTCTCCATGACTAAGTGGGTACCTACTCCAGTTGCTGTGGTAATTAGTGCAGCTGTATTTGCACTTGCACATCTCACTCCTGGAGAGTTTCCTCAGCTGTTTGTACTAGGGGCTGCTATGGGATTGTCGTATGCTCAAACTCGTAACCTTTTGACCCCTATCACAATACATGCTTTTTGGAACTCGGGAGTTATATTGCTTCTGACCTTCCTTCAGCTTCAAGGGTATGATATCAAGGAATTGTTGCAGGCAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abi

Protein Analysis

340

Amino Acids

36.97

Weight (kDa)

6.59

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rce1-like PF02517 231 - 296 1.2e-11 Type II CAAX prenyl endopeptidase Rce1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 781
AccB1I GGYRCC 1 cut(s) 781
AccI GTMKAC 3 cut(s) 8, 452, 523
AccII CGCG 1 cut(s) 750
AciI CCGC 8 cut(s) 158, 161, 183, 476, 496, 662, 748, 750
AclWI GGATC 3 cut(s) 506, 519, 679
AcoI YGGCCR 1 cut(s) 155
AcsI RAATTY 1 cut(s) 70
AcuI CTGAAG 1 cut(s) 959
AfaI GTAC 3 cut(s) 348, 783, 867
AfiI CCNNNNNNNGG 3 cut(s) 217, 356, 668
AgsI TTSAA 2 cut(s) 247, 983
AjnI CCWGG 1 cut(s) 841
AloI GAACNNNNNNTCC 2 cut(s) 728, 760
AluBI AGCT 8 cut(s) 29, 66, 434, 575, 654, 815, 859, 979
AluI AGCT 8 cut(s) 29, 66, 434, 575, 654, 815, 859, 979
Alw26I GTCTC 2 cut(s) 39, 769
AlwI GGATC 3 cut(s) 506, 519, 679
AlwNI CAGNNNCTG 1 cut(s) 797
Ama87I CYCGRG 1 cut(s) 946
AoxI GGCC 2 cut(s) 155, 184
ApeKI GCWGC 7 cut(s) 338, 431, 478, 575, 596, 812, 875
ApoI RAATTY 1 cut(s) 70
Asp718I GGTACC 1 cut(s) 781
AspS9I GGNCC 1 cut(s) 185
AsuC2I CCSGG 1 cut(s) 154
AvaI CYCGRG 1 cut(s) 946
BamHI GGATCC 1 cut(s) 511
BanI GGYRCC 1 cut(s) 781
BanII GRGCYC 1 cut(s) 624
BarI GAAGNNNNNNTAC 1 cut(s) 31
BbvCI CCTCAGC 1 cut(s) 855
BbvI GCAGC 7 cut(s) 350, 443, 465, 562, 608, 824, 862
BciT130I CCWGG 1 cut(s) 843
BcnI CCSGG 1 cut(s) 154
BcoDI GTCTC 2 cut(s) 39, 769
BfaI CTAG 3 cut(s) 26, 647, 869
BfmI CTRYAG 2 cut(s) 429, 570
Bme1390I CCNGG 2 cut(s) 154, 843
BmeT110I CYCGRG 1 cut(s) 946
BmgT120I GGNCC 1 cut(s) 185
BmiI GGNNCC 4 cut(s) 513, 621, 723, 783
BmrFI CCNGG 2 cut(s) 154, 843
BmsI GCATC 1 cut(s) 714
BpmI CTGGAG 3 cut(s) 732, 774, 864
Bpu10I CCTNAGC 1 cut(s) 855
BpuEI CTTGAG 3 cut(s) 399, 468, 752
BpuMI CCSGG 1 cut(s) 154
BsaI GGTCTC 1 cut(s) 769
BsaJI CCNNGG 2 cut(s) 263, 748
Bsc4I CCNNNNNNNGG 3 cut(s) 217, 356, 668
Bse1I ACTGG 3 cut(s) 300, 715, 791
BseBI CCWGG 1 cut(s) 843
BseDI CCNNGG 2 cut(s) 263, 748
BseGI GGATG 3 cut(s) 262, 391, 602
BseLI CCNNNNNNNGG 3 cut(s) 217, 356, 668
BseMII CTCAG 5 cut(s) 44, 157, 299, 387, 869
BseNI ACTGG 3 cut(s) 300, 715, 791
BseX3I CGGCCG 1 cut(s) 155
BseXI GCAGC 7 cut(s) 350, 443, 465, 562, 608, 824, 862
BseYI CCCAGC 1 cut(s) 62
BsgI GTGCAG 1 cut(s) 831
Bsh1236I CGCG 1 cut(s) 750
Bsh1285I CGRYCG 1 cut(s) 158
BshFI GGCC 2 cut(s) 157, 186
BshNI GGYRCC 1 cut(s) 781
BsiEI CGRYCG 1 cut(s) 158
BsiHKCI CYCGRG 1 cut(s) 946
BsiSI CCGG 2 cut(s) 154, 180
BslI CCNNNNNNNGG 3 cut(s) 217, 356, 668
BsmAI GTCTC 2 cut(s) 39, 769
BsmI GAATGC 1 cut(s) 366
BsnI GGCC 2 cut(s) 157, 186
Bso31I GGTCTC 1 cut(s) 769
BsoBI CYCGRG 1 cut(s) 946
Bsp1286I GDGCHC 1 cut(s) 624
Bsp143I GATC 2 cut(s) 511, 671
Bsp19I CCATGG 1 cut(s) 263
BspACI CCGC 8 cut(s) 158, 161, 183, 476, 496, 662, 748, 750
BspANI GGCC 2 cut(s) 157, 186
BspCNI CTCAG 5 cut(s) 43, 156, 300, 388, 868
BspFNI CGCG 1 cut(s) 750
BspLI GGNNCC 4 cut(s) 513, 621, 723, 783
BspMAI CTGCAG 1 cut(s) 433
BspPI GGATC 3 cut(s) 506, 519, 679
BspT107I GGYRCC 1 cut(s) 781
BspTNI GGTCTC 1 cut(s) 769
BsrI ACTGG 3 cut(s) 300, 715, 791
BssECI CCNNGG 2 cut(s) 263, 748
BssMI GATC 2 cut(s) 511, 671
BssNAI GTATAC 2 cut(s) 9, 453
BssT1I CCWWGG 1 cut(s) 263
Bst1107I GTATAC 2 cut(s) 9, 453
Bst2UI CCWGG 1 cut(s) 843
Bst4CI ACNGT 3 cut(s) 280, 457, 742
Bst6I CTCTTC 2 cut(s) 20, 242
BstAPI GCANNNNNTGC 1 cut(s) 693
BstC8I GCNNGC 3 cut(s) 463, 694, 1011
BstDEI CTNAG 6 cut(s) 30, 143, 308, 396, 774, 855
BstDSI CCRYGG 2 cut(s) 263, 748
BstF5I GGATG 3 cut(s) 262, 391, 602
BstFNI CGCG 1 cut(s) 750
BstKTI GATC 2 cut(s) 514, 674
BstMAI GTCTC 2 cut(s) 39, 769
BstMBI GATC 2 cut(s) 511, 671
BstMCI CGRYCG 1 cut(s) 158
BstMWI GCNNNNNNNGC 6 cut(s) 180, 575, 581, 693, 702, 821
BstNI CCWGG 1 cut(s) 843
BstNSI RCATGY 1 cut(s) 935
BstSCI CCNGG 2 cut(s) 152, 841
BstSFI CTRYAG 2 cut(s) 429, 570
BstUI CGCG 1 cut(s) 750
BstV1I GCAGC 7 cut(s) 350, 443, 465, 562, 608, 824, 862
BstX2I RGATCY 1 cut(s) 511
BstXI CCANNNNNNTGG 1 cut(s) 270
BstYI RGATCY 1 cut(s) 511
BstZ17I GTATAC 2 cut(s) 9, 453
BstZI CGGCCG 1 cut(s) 155
BsuRI GGCC 2 cut(s) 157, 186
BtgI CCRYGG 2 cut(s) 263, 748
BtsCI GGATG 3 cut(s) 262, 391, 602
BtsI GCAGTG 1 cut(s) 687
BtsIMutI CAGTG 5 cut(s) 285, 293, 619, 687, 708
Cac8I GCNNGC 3 cut(s) 463, 694, 1011
CaiI CAGNNNCTG 1 cut(s) 797
Cfr13I GGNCC 1 cut(s) 185
Cfr42I CCGCGG 1 cut(s) 751
CseI GACGC 1 cut(s) 650
Csp6I GTAC 3 cut(s) 347, 782, 866
CspCI CAANNNNNGTGG 2 cut(s) 515, 550
CviAII CATG 5 cut(s) 167, 264, 760, 769, 932
CviQI GTAC 3 cut(s) 347, 782, 866
DdeI CTNAG 6 cut(s) 30, 143, 308, 396, 774, 855
DpnI GATC 2 cut(s) 513, 673
DpnII GATC 2 cut(s) 511, 671
EaeI YGGCCR 1 cut(s) 155
EagI CGGCCG 1 cut(s) 155
Eam1104I CTCTTC 2 cut(s) 20, 242
EarI CTCTTC 2 cut(s) 20, 242
EclXI CGGCCG 1 cut(s) 155
Eco130I CCWWGG 1 cut(s) 263
Eco24I GRGCYC 1 cut(s) 624
Eco31I GGTCTC 1 cut(s) 769
Eco32I GATATC 1 cut(s) 994
Eco52I CGGCCG 1 cut(s) 155
Eco57I CTGAAG 1 cut(s) 959
Eco88I CYCGRG 1 cut(s) 946
EcoRII CCWGG 1 cut(s) 841
EcoRV GATATC 1 cut(s) 994
EcoT14I CCWWGG 1 cut(s) 263
EcoT38I GRGCYC 1 cut(s) 624
ErhI CCWWGG 1 cut(s) 263
FaeI CATG 5 cut(s) 170, 267, 763, 772, 935
FatI CATG 5 cut(s) 166, 263, 759, 768, 931
FauI CCCGC 2 cut(s) 669, 743
FblI GTMKAC 3 cut(s) 8, 452, 523
FokI GGATG 3 cut(s) 269, 398, 589
FriOI GRGCYC 1 cut(s) 624
FspBI CTAG 3 cut(s) 26, 647, 869
GsaI CCCAGC 1 cut(s) 66
GsuI CTGGAG 3 cut(s) 732, 774, 864
HaeIII GGCC 2 cut(s) 157, 186
HapII CCGG 2 cut(s) 154, 180
HgaI GACGC 1 cut(s) 650
Hin1II CATG 5 cut(s) 170, 267, 763, 772, 935
HincII GTYRAC 1 cut(s) 588
HindII GTYRAC 1 cut(s) 588
HpaII CCGG 2 cut(s) 154, 180
Hpy166II GTNNAC 4 cut(s) 9, 453, 524, 588
Hpy188I TCNGA 2 cut(s) 146, 966
Hpy188III TCNNGA 4 cut(s) 371, 485, 731, 948
Hpy8I GTNNAC 4 cut(s) 9, 453, 524, 588
HpyAV CCTTC 7 cut(s) 50, 94, 221, 385, 631, 979, 983
HpyCH4III ACNGT 3 cut(s) 280, 457, 742
HpyCH4V TGCA 7 cut(s) 238, 431, 461, 812, 824, 830, 1009
HpyF10VI GCNNNNNNNGC 6 cut(s) 180, 575, 581, 693, 702, 821
HpyF3I CTNAG 6 cut(s) 30, 143, 308, 396, 774, 855
Hsp92II CATG 5 cut(s) 170, 267, 763, 772, 935
KpnI GGTACC 1 cut(s) 785
KspI CCGCGG 1 cut(s) 751
Kzo9I GATC 2 cut(s) 511, 671
LmnI GCTCC 2 cut(s) 619, 727
Lsp1109I GCAGC 7 cut(s) 350, 443, 465, 562, 608, 824, 862
LweI GCATC 1 cut(s) 714
MaeI CTAG 3 cut(s) 26, 647, 869
MaeIII GTNAC 2 cut(s) 421, 905
MalI GATC 2 cut(s) 513, 673
MboI GATC 2 cut(s) 511, 671
MboII GAAGA 2 cut(s) 7, 259
MflI RGATCY 1 cut(s) 511
MhlI GDGCHC 1 cut(s) 624
MluCI AATT 5 cut(s) 70, 666, 726, 804, 1001
MmeI TCCRAC 1 cut(s) 496
MseI TTAA 1 cut(s) 68
MslI CAYNNNNRTG 1 cut(s) 268
MspA1I CMGCKG 4 cut(s) 478, 750, 815, 859
MspI CCGG 2 cut(s) 154, 180
MspR9I CCNGG 2 cut(s) 154, 843
Mva1269I GAATGC 1 cut(s) 366
MvaI CCWGG 1 cut(s) 843
MvnI CGCG 1 cut(s) 750
MwoI GCNNNNNNNGC 6 cut(s) 180, 575, 581, 693, 702, 821
NciI CCSGG 1 cut(s) 154
NcoI CCATGG 1 cut(s) 263
NdeII GATC 2 cut(s) 511, 671
NlaIII CATG 5 cut(s) 170, 267, 763, 772, 935
NlaIV GGNNCC 4 cut(s) 513, 621, 723, 783
NspI RCATGY 1 cut(s) 935
PctI GAATGC 1 cut(s) 366
PfoI TCCNGGA 1 cut(s) 841
Psp6I CCWGG 1 cut(s) 841
PspFI CCCAGC 1 cut(s) 62
PspGI CCWGG 1 cut(s) 841
PspN4I GGNNCC 4 cut(s) 513, 621, 723, 783
PspPI GGNCC 1 cut(s) 185
PstI CTGCAG 1 cut(s) 433
PstNI CAGNNNCTG 1 cut(s) 797
PsuI RGATCY 1 cut(s) 511
PvuII CAGCTG 2 cut(s) 815, 859
RsaI GTAC 3 cut(s) 348, 783, 867
RsaNI GTAC 3 cut(s) 347, 782, 866
RseI CAYNNNNRTG 1 cut(s) 268
SacII CCGCGG 1 cut(s) 751
SaqAI TTAA 1 cut(s) 68
Sau3AI GATC 2 cut(s) 511, 671
Sau96I GGNCC 1 cut(s) 185
ScrFI CCNGG 2 cut(s) 154, 843
SduI GDGCHC 1 cut(s) 624
SfaNI GCATC 1 cut(s) 714
SfcI CTRYAG 2 cut(s) 429, 570
Sfr303I CCGCGG 1 cut(s) 751
SgrBI CCGCGG 1 cut(s) 751
SmiMI CAYNNNNRTG 1 cut(s) 268
SmlI CTYRAG 3 cut(s) 414, 483, 731
SmoI CTYRAG 3 cut(s) 414, 483, 731
Sse9I AATT 5 cut(s) 70, 666, 726, 804, 1001
SsiI CCGC 8 cut(s) 158, 161, 183, 476, 496, 662, 748, 750
SspMI CTAG 3 cut(s) 26, 647, 869
StyD4I CCNGG 2 cut(s) 152, 841
StyI CCWWGG 1 cut(s) 263
TaaI ACNGT 3 cut(s) 280, 457, 742
TasI AATT 5 cut(s) 70, 666, 726, 804, 1001
TatI WGTACW 1 cut(s) 865
TauI GCSGC 3 cut(s) 160, 163, 186
Tru1I TTAA 1 cut(s) 68
Tru9I TTAA 1 cut(s) 68
TscAI CASTG 5 cut(s) 285, 300, 619, 694, 715
TseI GCWGC 7 cut(s) 338, 431, 478, 575, 596, 812, 875
TspDTI ATGAA 3 cut(s) 155, 240, 748
TspRI CASTG 5 cut(s) 285, 300, 619, 694, 715
XapI RAATTY 1 cut(s) 70
XceI RCATGY 1 cut(s) 935
XmiI GTMKAC 3 cut(s) 8, 452, 523
XspI CTAG 3 cut(s) 26, 647, 869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.