Rw7G025690

CAAX protease self-immunity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0004201
Physical Location & Seq
Reverse (-)
32229710 .. 32232387
2678 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G025690.1

Sequence Viewer

Length: 600 bp
ATGGAAAATGAAGGGGGCTTGCAAATTTTAAAGAGGTGGGATGTGCCTTGGGAATGGCAAACAGTGTCATTGACATCACTGGCTTGTGGACTGAGTTCTGTTTTGACAGGATTGGTAGAGGCAGCAGTTGTACCCTATTTAGGATTGAGCATTCAGGATTTGAGTCTAGATGAGAAGGCAGAGTTTCTGTTGGTTGCTCAAGGTGTTACGACTGCAGCCATACTTGGAGTTCTGTATACCGTTGCCGGCAAATTCCAACCGTTGCCTCAAGATATTTATCGCTATGATTGTATTAGCTTGTACTGTTGCAGATTGGAGGGAACCTTTCAGTCTACCAAGAGGGTGACTTTTGTGGGCTGGGATTGGTCTTGTTGGTGCTGTAGTTGCTGTGACATTGACAGGGGCTGCCACGTCCTTTTTCAGTGGGCAAACCCCAGAAAGAGAGATCAAAGCTTTTGGGATGATATTGAGGATGGGCCAAGGCCTTTGGGTCCTTTTGGCTTACAACAGTGGGCGGTAAAGGATCGGAAATGGTATACCCAACAACAACTTGACGAGGTCCGCATTGAAGTAGCCGACTACTTACAGACTCTGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abi

Protein Analysis

199

Amino Acids

22.73

Weight (kDa)

4.64

Isoelectric Point (pI)

49.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 236, 332, 536
AciI CCGC 2 cut(s) 515, 562
AclWI GGATC 1 cut(s) 531
AcsI RAATTY 2 cut(s) 24, 251
AfaI GTAC 2 cut(s) 132, 302
AfiI CCNNNNNNNGG 1 cut(s) 140
AgsI TTSAA 1 cut(s) 569
AjiI CACGTC 1 cut(s) 412
AluBI AGCT 2 cut(s) 297, 453
AluI AGCT 2 cut(s) 297, 453
AlwI GGATC 1 cut(s) 531
AlwNI CAGNNNCTG 2 cut(s) 405, 592
AoxI GGCC 2 cut(s) 476, 482
ApeKI GCWGC 3 cut(s) 122, 215, 405
ApoI RAATTY 2 cut(s) 24, 251
AspS9I GGNCC 3 cut(s) 476, 491, 559
AsuHPI GGTGA 1 cut(s) 355
AvaII GGWCC 2 cut(s) 491, 559
BbvI GCAGC 3 cut(s) 134, 227, 392
BccI CCATC 1 cut(s) 467
BfaI CTAG 1 cut(s) 167
BfmI CTRYAG 3 cut(s) 213, 379, 596
BisI GCNGC 3 cut(s) 123, 216, 406
BlsI GCNGC 3 cut(s) 124, 217, 407
Bme18I GGWCC 2 cut(s) 491, 559
BmgBI CACGTC 1 cut(s) 412
BmgT120I GGNCC 3 cut(s) 476, 491, 559
BmiI GGNNCC 2 cut(s) 322, 492
BpuEI CTTGAG 2 cut(s) 183, 252
BsaBI GATNNNNATC 1 cut(s) 276
BsaJI CCNNGG 2 cut(s) 47, 479
Bsc4I CCNNNNNNNGG 1 cut(s) 140
Bse118I RCCGGY 1 cut(s) 245
Bse1I ACTGG 1 cut(s) 84
Bse8I GATNNNNATC 1 cut(s) 276
BseDI CCNNGG 2 cut(s) 47, 479
BseGI GGATG 3 cut(s) 46, 466, 478
BseJI GATNNNNATC 1 cut(s) 276
BseLI CCNNNNNNNGG 1 cut(s) 140
BseMII CTCAG 1 cut(s) 83
BseNI ACTGG 1 cut(s) 84
BseXI GCAGC 3 cut(s) 134, 227, 392
BseYI CCCAGC 1 cut(s) 357
BshFI GGCC 2 cut(s) 478, 484
BsiSI CCGG 1 cut(s) 246
BslI CCNNNNNNNGG 1 cut(s) 140
BsmI GAATGC 1 cut(s) 150
BsnI GGCC 2 cut(s) 478, 484
Bsp143I GATC 2 cut(s) 445, 523
BspACI CCGC 2 cut(s) 515, 562
BspANI GGCC 2 cut(s) 478, 484
BspCNI CTCAG 1 cut(s) 84
BspLI GGNNCC 2 cut(s) 322, 492
BspMAI CTGCAG 1 cut(s) 217
BspPI GGATC 1 cut(s) 531
BsrFI RCCGGY 1 cut(s) 245
BsrI ACTGG 1 cut(s) 84
BssAI RCCGGY 1 cut(s) 245
BssECI CCNNGG 2 cut(s) 47, 479
BssMI GATC 2 cut(s) 445, 523
BssNAI GTATAC 2 cut(s) 237, 537
BssT1I CCWWGG 2 cut(s) 47, 479
Bst1107I GTATAC 2 cut(s) 237, 537
Bst4CI ACNGT 5 cut(s) 64, 241, 261, 305, 510
BstC8I GCNNGC 2 cut(s) 20, 247
BstDEI CTNAG 1 cut(s) 92
BstF5I GGATG 3 cut(s) 46, 466, 478
BstKTI GATC 2 cut(s) 448, 526
BstMBI GATC 2 cut(s) 445, 523
BstMWI GCNNNNNNNGC 1 cut(s) 384
BstSFI CTRYAG 3 cut(s) 213, 379, 596
BstV1I GCAGC 3 cut(s) 134, 227, 392
BstZ17I GTATAC 2 cut(s) 237, 537
BsuRI GGCC 2 cut(s) 478, 484
BtrI CACGTC 1 cut(s) 412
BtsCI GGATG 3 cut(s) 46, 466, 478
BtsIMutI CAGTG 4 cut(s) 69, 77, 428, 515
Cac8I GCNNGC 2 cut(s) 20, 247
CaiI CAGNNNCTG 2 cut(s) 405, 592
Cfr10I RCCGGY 1 cut(s) 245
Cfr13I GGNCC 3 cut(s) 476, 491, 559
Csp6I GTAC 2 cut(s) 131, 301
CviQI GTAC 2 cut(s) 131, 301
DdeI CTNAG 1 cut(s) 92
DpnI GATC 2 cut(s) 447, 525
DpnII GATC 2 cut(s) 445, 523
DraI TTTAAA 1 cut(s) 30
Eco130I CCWWGG 2 cut(s) 47, 479
Eco147I AGGCCT 1 cut(s) 484
Eco47I GGWCC 2 cut(s) 491, 559
EcoO109I RGGNCCY 1 cut(s) 491
EcoT14I CCWWGG 2 cut(s) 47, 479
ErhI CCWWGG 2 cut(s) 47, 479
FaiI YATR 4 cut(s) 221, 237, 285, 537
FblI GTMKAC 3 cut(s) 236, 332, 536
Fnu4HI GCNGC 3 cut(s) 123, 216, 406
FokI GGATG 3 cut(s) 53, 473, 485
Fsp4HI GCNGC 3 cut(s) 123, 216, 406
FspBI CTAG 1 cut(s) 167
GluI GCNGC 3 cut(s) 123, 216, 406
GsaI CCCAGC 1 cut(s) 361
HaeIII GGCC 2 cut(s) 478, 484
HapII CCGG 1 cut(s) 246
HindIII AAGCTT 1 cut(s) 451
HinfI GANTC 2 cut(s) 163, 589
HpaII CCGG 1 cut(s) 246
HphI GGTGA 1 cut(s) 355
Hpy166II GTNNAC 4 cut(s) 89, 237, 333, 537
Hpy188I TCNGA 1 cut(s) 528
Hpy188III TCNNGA 3 cut(s) 155, 167, 269
Hpy8I GTNNAC 4 cut(s) 89, 237, 333, 537
HpyAV CCTTC 2 cut(s) 5, 169
HpyCH4III ACNGT 5 cut(s) 64, 241, 261, 305, 510
HpyCH4IV ACGT 1 cut(s) 411
HpyCH4V TGCA 3 cut(s) 22, 215, 309
HpyF10VI GCNNNNNNNGC 1 cut(s) 384
HpyF3I CTNAG 1 cut(s) 92
HpySE526I ACGT 1 cut(s) 411
KroI GCCGGC 1 cut(s) 245
KroNI GCCGGC 1 cut(s) 247
Kzo9I GATC 2 cut(s) 445, 523
LpnPI CCDG 7 cut(s) 65, 93, 140, 259, 343, 385, 448
Lsp1109I GCAGC 3 cut(s) 134, 227, 392
MaeI CTAG 1 cut(s) 167
MaeII ACGT 1 cut(s) 411
MaeIII GTNAC 3 cut(s) 205, 343, 389
MalI GATC 2 cut(s) 447, 525
MboI GATC 2 cut(s) 445, 523
MluCI AATT 2 cut(s) 24, 251
MlyI GAGTC 2 cut(s) 172, 583
MmeI TCCRAC 1 cut(s) 280
MnlI CCTC 7 cut(s) 27, 112, 276, 310, 333, 463, 550
MroNI GCCGGC 1 cut(s) 245
MseI TTAA 1 cut(s) 29
MspI CCGG 1 cut(s) 246
Mva1269I GAATGC 1 cut(s) 150
MwoI GCNNNNNNNGC 1 cut(s) 384
NaeI GCCGGC 1 cut(s) 247
NdeII GATC 2 cut(s) 445, 523
NgoMIV GCCGGC 1 cut(s) 245
NlaIV GGNNCC 2 cut(s) 322, 492
NmuCI GTSAC 2 cut(s) 343, 389
PceI AGGCCT 1 cut(s) 484
PctI GAATGC 1 cut(s) 150
PdiI GCCGGC 1 cut(s) 247
PflFI GACNNNGTC 1 cut(s) 557
PkrI GCNGC 3 cut(s) 124, 217, 407
PleI GAGTC 2 cut(s) 171, 583
PpsI GAGTC 2 cut(s) 171, 583
PpuMI RGGWCCY 1 cut(s) 491
Psp5II RGGWCCY 1 cut(s) 491
PspFI CCCAGC 1 cut(s) 357
PspN4I GGNNCC 2 cut(s) 322, 492
PspPI GGNCC 3 cut(s) 476, 491, 559
PspPPI RGGWCCY 1 cut(s) 491
PstI CTGCAG 1 cut(s) 217
PstNI CAGNNNCTG 2 cut(s) 405, 592
PsyI GACNNNGTC 1 cut(s) 557
RsaI GTAC 2 cut(s) 132, 302
RsaNI GTAC 2 cut(s) 131, 301
SaqAI TTAA 1 cut(s) 29
SatI GCNGC 3 cut(s) 123, 216, 406
Sau3AI GATC 2 cut(s) 445, 523
Sau96I GGNCC 3 cut(s) 476, 491, 559
SchI GAGTC 2 cut(s) 172, 583
SetI ASST 7 cut(s) 38, 205, 299, 326, 414, 455, 561
SfcI CTRYAG 3 cut(s) 213, 379, 596
SinI GGWCC 2 cut(s) 491, 559
SmlI CTYRAG 2 cut(s) 198, 267
SmoI CTYRAG 2 cut(s) 198, 267
Sse9I AATT 2 cut(s) 24, 251
SseBI AGGCCT 1 cut(s) 484
SsiI CCGC 2 cut(s) 515, 562
SspMI CTAG 1 cut(s) 167
StuI AGGCCT 1 cut(s) 484
StyI CCWWGG 2 cut(s) 47, 479
TaaI ACNGT 5 cut(s) 64, 241, 261, 305, 510
TaiI ACGT 1 cut(s) 414
TasI AATT 2 cut(s) 24, 251
TatI WGTACW 1 cut(s) 300
Tru1I TTAA 1 cut(s) 29
Tru9I TTAA 1 cut(s) 29
TscAI CASTG 4 cut(s) 69, 84, 428, 515
TseFI GTSAC 2 cut(s) 343, 389
TseI GCWGC 3 cut(s) 122, 215, 405
Tsp45I GTSAC 2 cut(s) 343, 389
TspDTI ATGAA 1 cut(s) 24
TspRI CASTG 4 cut(s) 69, 84, 428, 515
Tth111I GACNNNGTC 1 cut(s) 557
VpaK11BI GGWCC 2 cut(s) 491, 559
XapI RAATTY 2 cut(s) 24, 251
XbaI TCTAGA 1 cut(s) 166
XmiI GTMKAC 3 cut(s) 236, 332, 536
XspI CTAG 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.