MD13G1072600.v1.1

CAAX protease self-immunity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004201
Physical Location & Seq
Reverse (-)
5103414 .. 5106588
3175 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1072600.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGCTTAAAAGTTCGAATACAATATCAAAACCAGCAATGGTGTGCACGCTGTTCACATCTGTAGCTCAATCTTCCTTCTCCTGCTTCTCGGACTCACCCAAGCCAACTTCCTCCAAAACCCAAATCCCCTCCCTTTTTCTCGACGGCCGCAGAAGCTTCACCGCCGGCTTCTCGCCGCGCCGATCGACCCAGCTTTCAAGGCTCAGAAACGTCAGAAATCCGCCGTACATTTGCTTTTTCAGCGGCGGAGGAAAGCCCAAAGGCGATTTCCAAGAAAAGGAAAATGAATCGCAGTGGCTGATTTTGAAGCGGTGGGATGTCCCCTGGGAATGGCAAACGGCGTCGTTAACATCGCTTGCTTGTGGGCTGAGTTTTGTTTTGACAGGACTGGTAGAGGCGGCAGCTATACCGTATTTAGGAATTCGAATTCAGGATTTGAGTTTGGATGAGAAGGCAGAGATACTGTTTCTTGATCAAGGTATTACAACTGCAGCTGTACTTGCTGTTCTATATACCGTTGCCAACACTTACCAACCACTGCCTGAAGATGTTTATCGCTACGATTTGAAGGAACCTTTCAATCTACAGAAGGGATGGCTATTGTGGGCAGGGATTGGTTTTGTTGGTGCCATAGCTGCTATTGCAGTGACAGGGGCTGCATTGTCCATGTTGAGGGGGGAGAGCCCAGAACGAGAGAAGGACGCTCTAGTAAGTTTGCTTCCACTGATTGGATCGTCCGGTATTAGTACTGCTTGCTTGCTAGGCATCACTGGAGTTTTGGCTCCAATTCTCGAAGAAACTGTGTTTCGCGGGTTTTTTATGGTCTCCATGACTAAATGGGTACCTACACCAGTGGCTATTGTAATAAGTGCCGCTGTATTTGCACTTGCACATCTCACTCCTGGAGAGTTTCCCCAGCTGTTTGTGCTAGGCACTGCTCTGGGATTGTCGTATGCTCAAACTCGCAACCTTGTAACTCCCATCACAATACATGCTCTCTGGAACTCAGGAGTGATCTTGCTTCTGACGTTCCTTCAGCTACAAGGGTACGATATCAAGGAACTGTTGCAGACAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abi

Protein Analysis

360

Amino Acids

39.12

Weight (kDa)

7.58

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rce1-like PF02517 251 - 336 4.3e-21 Type II CAAX prenyl endopeptidase Rce1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 841
AccB1I GGYRCC 2 cut(s) 626, 841
AccB7I CCANNNNNTGG 1 cut(s) 728
AccII CGCG 2 cut(s) 178, 810
AclWI GGATC 1 cut(s) 739
AcoI YGGCCR 1 cut(s) 145
AcsI RAATTY 2 cut(s) 420, 426
AcuI CTGAAG 2 cut(s) 564, 1019
AcyI GRCGYC 1 cut(s) 341
AfaI GTAC 5 cut(s) 227, 498, 748, 843, 1049
AfiI CCNNNNNNNGG 5 cut(s) 277, 330, 416, 672, 728
AgsI TTSAA 4 cut(s) 198, 307, 568, 580
AjnI CCWGG 2 cut(s) 323, 901
AjuI GAANNNNNNNTTGG 2 cut(s) 251, 283
AluBI AGCT 8 cut(s) 65, 156, 193, 404, 494, 635, 919, 1039
AluI AGCT 8 cut(s) 65, 156, 193, 404, 494, 635, 919, 1039
Alw21I GWGCWC 1 cut(s) 47
Alw26I GTCTC 1 cut(s) 829
Alw44I GTGCAC 1 cut(s) 43
AlwI GGATC 1 cut(s) 739
AlwNI CAGNNNCTG 2 cut(s) 298, 656
AoxI GGCC 1 cut(s) 145
ApaLI GTGCAC 1 cut(s) 43
ApeKI GCWGC 4 cut(s) 401, 491, 635, 656
ApoI RAATTY 2 cut(s) 420, 426
Asp718I GGTACC 1 cut(s) 841
AspLEI GCGC 1 cut(s) 180
AsuHPI GGTGA 2 cut(s) 87, 151
AsuII TTCGAA 2 cut(s) 14, 424
BaeGI GKGCMC 1 cut(s) 47
BanI GGYRCC 2 cut(s) 626, 841
BanII GRGCYC 1 cut(s) 686
BarI GAAGNNNNNNTAC 2 cut(s) 702, 734
Bbv12I GWGCWC 1 cut(s) 47
BbvI GCAGC 4 cut(s) 413, 503, 622, 643
BccI CCATC 2 cut(s) 588, 989
BceAI ACGGC 3 cut(s) 160, 208, 354
BciT130I CCWGG 2 cut(s) 325, 903
BclI TGATCA 1 cut(s) 472
BcoDI GTCTC 1 cut(s) 829
BfaI CTAG 3 cut(s) 707, 761, 929
BfmI CTRYAG 3 cut(s) 60, 489, 584
BisI GCNGC 9 cut(s) 148, 176, 244, 399, 402, 492, 636, 657, 873
BlsI GCNGC 9 cut(s) 149, 177, 245, 400, 403, 493, 637, 658, 874
BmcAI AGTACT 1 cut(s) 748
Bme1390I CCNGG 2 cut(s) 325, 903
BmiI GGNNCC 4 cut(s) 573, 628, 783, 843
BmrFI CCNGG 2 cut(s) 325, 903
BmsI GCATC 1 cut(s) 774
BpmI CTGGAG 2 cut(s) 792, 924
Bpu14I TTCGAA 2 cut(s) 14, 424
BsaBI GATNNNNATC 1 cut(s) 552
BsaHI GRCGYC 1 cut(s) 341
BsaI GGTCTC 1 cut(s) 829
BsaJI CCNNGG 2 cut(s) 323, 324
BsaWI WCCGGW 1 cut(s) 737
Bsc4I CCNNNNNNNGG 5 cut(s) 277, 330, 416, 672, 728
Bse118I RCCGGY 1 cut(s) 164
Bse1I ACTGG 3 cut(s) 393, 775, 851
Bse3DI GCAATG 1 cut(s) 42
Bse8I GATNNNNATC 1 cut(s) 552
BseBI CCWGG 2 cut(s) 325, 903
BseDI CCNNGG 2 cut(s) 323, 324
BseGI GGATG 3 cut(s) 322, 451, 599
BseJI GATNNNNATC 1 cut(s) 552
BseLI CCNNNNNNNGG 5 cut(s) 277, 330, 416, 672, 728
BseMI GCAATG 1 cut(s) 42
BseMII CTCAG 3 cut(s) 217, 359, 1020
BseNI ACTGG 3 cut(s) 393, 775, 851
BseSI GKGCMC 1 cut(s) 47
BseX3I CGGCCG 1 cut(s) 145
BseXI GCAGC 4 cut(s) 413, 503, 622, 643
BseYI CCCAGC 2 cut(s) 189, 915
Bsh1236I CGCG 2 cut(s) 178, 810
Bsh1285I CGRYCG 2 cut(s) 148, 185
BshFI GGCC 1 cut(s) 147
BshNI GGYRCC 2 cut(s) 626, 841
BsiEI CGRYCG 2 cut(s) 148, 185
BsiHKAI GWGCWC 1 cut(s) 47
BsiSI CCGG 2 cut(s) 165, 738
BslFI GGGAC 1 cut(s) 305
BslI CCNNNNNNNGG 5 cut(s) 277, 330, 416, 672, 728
BsmAI GTCTC 1 cut(s) 829
BsmFI GGGAC 1 cut(s) 305
BsnI GGCC 1 cut(s) 147
Bso31I GGTCTC 1 cut(s) 829
Bsp119I TTCGAA 2 cut(s) 14, 424
Bsp1286I GDGCHC 2 cut(s) 47, 686
Bsp143I GATC 4 cut(s) 182, 472, 731, 1014
BspANI GGCC 1 cut(s) 147
BspCNI CTCAG 3 cut(s) 216, 360, 1019
BspFNI CGCG 2 cut(s) 178, 810
BspLI GGNNCC 4 cut(s) 573, 628, 783, 843
BspMAI CTGCAG 1 cut(s) 493
BspPI GGATC 1 cut(s) 739
BspT104I TTCGAA 2 cut(s) 14, 424
BspT107I GGYRCC 2 cut(s) 626, 841
BspTNI GGTCTC 1 cut(s) 829
BsrDI GCAATG 1 cut(s) 42
BsrFI RCCGGY 1 cut(s) 164
BsrI ACTGG 3 cut(s) 393, 775, 851
BssAI RCCGGY 1 cut(s) 164
BssECI CCNNGG 2 cut(s) 323, 324
BssMI GATC 4 cut(s) 182, 472, 731, 1014
BssNI GRCGYC 1 cut(s) 341
Bst2UI CCWGG 2 cut(s) 325, 903
Bst4CI ACNGT 5 cut(s) 411, 465, 517, 802, 1065
BstACI GRCGYC 1 cut(s) 341
BstBI TTCGAA 2 cut(s) 14, 424
BstC8I GCNNGC 5 cut(s) 47, 166, 357, 754, 758
BstDEI CTNAG 3 cut(s) 203, 368, 1006
BstF5I GGATG 3 cut(s) 322, 451, 599
BstFNI CGCG 2 cut(s) 178, 810
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 4 cut(s) 185, 475, 734, 1017
BstMAI GTCTC 1 cut(s) 829
BstMBI GATC 4 cut(s) 182, 472, 731, 1014
BstMCI CGRYCG 2 cut(s) 148, 185
BstMWI GCNNNNNNNGC 9 cut(s) 153, 199, 240, 500, 635, 641, 762, 881, 925
BstNI CCWGG 2 cut(s) 325, 903
BstNSI RCATGY 1 cut(s) 995
BstSCI CCNGG 2 cut(s) 323, 901
BstSFI CTRYAG 3 cut(s) 60, 489, 584
BstSLI GKGCMC 1 cut(s) 47
BstUI CGCG 2 cut(s) 178, 810
BstV1I GCAGC 4 cut(s) 413, 503, 622, 643
BstZI CGGCCG 1 cut(s) 145
BsuRI GGCC 1 cut(s) 147
BtgZI GCGATG 1 cut(s) 336
BtsCI GGATG 3 cut(s) 322, 451, 599
BtsI GCAGTG 4 cut(s) 299, 536, 651, 933
BtsIMutI CAGTG 7 cut(s) 299, 536, 651, 722, 768, 858, 933
Cac8I GCNNGC 5 cut(s) 47, 166, 357, 754, 758
CaiI CAGNNNCTG 2 cut(s) 298, 656
CfoI GCGC 1 cut(s) 180
Cfr10I RCCGGY 1 cut(s) 164
CseI GACGC 2 cut(s) 330, 710
Csp6I GTAC 5 cut(s) 226, 497, 747, 842, 1048
CviAII CATG 3 cut(s) 667, 829, 992
CviQI GTAC 5 cut(s) 226, 497, 747, 842, 1048
DdeI CTNAG 3 cut(s) 203, 368, 1006
DpnI GATC 4 cut(s) 184, 474, 733, 1016
DpnII GATC 4 cut(s) 182, 472, 731, 1014
EaeI YGGCCR 1 cut(s) 145
EagI CGGCCG 1 cut(s) 145
EciI GGCGGA 2 cut(s) 210, 261
EclXI CGGCCG 1 cut(s) 145
Eco24I GRGCYC 1 cut(s) 686
Eco31I GGTCTC 1 cut(s) 829
Eco32I GATATC 1 cut(s) 1054
Eco52I CGGCCG 1 cut(s) 145
Eco57I CTGAAG 2 cut(s) 564, 1019
EcoRI GAATTC 2 cut(s) 420, 426
EcoRII CCWGG 2 cut(s) 323, 901
EcoRV GATATC 1 cut(s) 1054
EcoT38I GRGCYC 1 cut(s) 686
FaeI CATG 3 cut(s) 670, 832, 995
FaiI YATR 9 cut(s) 407, 511, 513, 632, 668, 821, 830, 954, 993
FaqI GGGAC 1 cut(s) 305
FatI CATG 3 cut(s) 666, 828, 991
FauI CCCGC 1 cut(s) 803
FbaI TGATCA 1 cut(s) 472
Fnu4HI GCNGC 9 cut(s) 148, 176, 244, 399, 402, 492, 636, 657, 873
FokI GGATG 3 cut(s) 329, 458, 606
FriOI GRGCYC 1 cut(s) 686
Fsp4HI GCNGC 9 cut(s) 148, 176, 244, 399, 402, 492, 636, 657, 873
FspBI CTAG 3 cut(s) 707, 761, 929
GlaI GCGC 1 cut(s) 179
GluI GCNGC 9 cut(s) 148, 176, 244, 399, 402, 492, 636, 657, 873
GsaI CCCAGC 2 cut(s) 193, 919
GsuI CTGGAG 2 cut(s) 792, 924
HaeIII GGCC 1 cut(s) 147
HapII CCGG 2 cut(s) 165, 738
HgaI GACGC 2 cut(s) 330, 710
HhaI GCGC 1 cut(s) 180
Hin1I GRCGYC 1 cut(s) 341
Hin1II CATG 3 cut(s) 670, 832, 995
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HincII GTYRAC 1 cut(s) 348
HindII GTYRAC 1 cut(s) 348
HindIII AAGCTT 1 cut(s) 154
HinfI GANTC 2 cut(s) 92, 287
HpaI GTTAAC 1 cut(s) 348
HpaII CCGG 2 cut(s) 165, 738
HphI GGTGA 2 cut(s) 87, 151
Hpy166II GTNNAC 3 cut(s) 45, 54, 348
Hpy188I TCNGA 4 cut(s) 91, 206, 215, 1026
Hpy188III TCNNGA 6 cut(s) 140, 431, 470, 791, 1000, 1008
Hpy8I GTNNAC 3 cut(s) 45, 54, 348
Hpy99I CGWCG 2 cut(s) 146, 346
HpyAV CCTTC 6 cut(s) 85, 445, 562, 583, 691, 1043
HpyCH4III ACNGT 5 cut(s) 411, 465, 517, 802, 1065
HpyCH4IV ACGT 2 cut(s) 210, 1028
HpyCH4V TGCA 7 cut(s) 45, 491, 644, 659, 884, 890, 1069
HpyF10VI GCNNNNNNNGC 9 cut(s) 153, 199, 240, 500, 635, 641, 762, 881, 925
HpyF3I CTNAG 3 cut(s) 203, 368, 1006
HpySE526I ACGT 2 cut(s) 210, 1028
Hsp92I GRCGYC 1 cut(s) 341
Hsp92II CATG 3 cut(s) 670, 832, 995
HspAI GCGC 1 cut(s) 178
KpnI GGTACC 1 cut(s) 845
KroI GCCGGC 1 cut(s) 164
KroNI GCCGGC 1 cut(s) 166
Ksp22I TGATCA 1 cut(s) 472
KspAI GTTAAC 1 cut(s) 348
Kzo9I GATC 4 cut(s) 182, 472, 731, 1014
LmnI GCTCC 1 cut(s) 787
Lsp1109I GCAGC 4 cut(s) 413, 503, 622, 643
LweI GCATC 1 cut(s) 774
MaeI CTAG 3 cut(s) 707, 761, 929
MaeII ACGT 2 cut(s) 210, 1028
MaeIII GTNAC 2 cut(s) 646, 973
MalI GATC 4 cut(s) 184, 474, 733, 1016
MboI GATC 4 cut(s) 182, 472, 731, 1014
MboII GAAGA 3 cut(s) 63, 557, 806
MhlI GDGCHC 2 cut(s) 47, 686
MluCI AATT 3 cut(s) 420, 426, 786
MlyI GAGTC 1 cut(s) 86
MnlI CCTC 5 cut(s) 121, 139, 242, 388, 666
MroNI GCCGGC 1 cut(s) 164
MseI TTAA 2 cut(s) 6, 347
MspA1I CMGCKG 4 cut(s) 243, 494, 875, 919
MspI CCGG 2 cut(s) 165, 738
MspR9I CCNGG 2 cut(s) 325, 903
MvaI CCWGG 2 cut(s) 325, 903
MvnI CGCG 2 cut(s) 178, 810
MwoI GCNNNNNNNGC 9 cut(s) 153, 199, 240, 500, 635, 641, 762, 881, 925
NaeI GCCGGC 1 cut(s) 166
NdeII GATC 4 cut(s) 182, 472, 731, 1014
NgoMIV GCCGGC 1 cut(s) 164
NlaIII CATG 3 cut(s) 670, 832, 995
NlaIV GGNNCC 4 cut(s) 573, 628, 783, 843
NmuCI GTSAC 1 cut(s) 646
NspI RCATGY 1 cut(s) 995
NspV TTCGAA 2 cut(s) 14, 424
PasI CCCWGGG 1 cut(s) 324
PdiI GCCGGC 1 cut(s) 166
PfeI GAWTC 1 cut(s) 287
PflMI CCANNNNNTGG 1 cut(s) 728
PfoI TCCNGGA 1 cut(s) 901
PkrI GCNGC 9 cut(s) 149, 177, 245, 400, 403, 493, 637, 658, 874
Ple19I CGATCG 1 cut(s) 185
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
Psp6I CCWGG 2 cut(s) 323, 901
PspFI CCCAGC 2 cut(s) 189, 915
PspGI CCWGG 2 cut(s) 323, 901
PspN4I GGNNCC 4 cut(s) 573, 628, 783, 843
PsrI GAACNNNNNNTAC 2 cut(s) 489, 521
PstI CTGCAG 1 cut(s) 493
PstNI CAGNNNCTG 2 cut(s) 298, 656
PvuI CGATCG 1 cut(s) 185
PvuII CAGCTG 2 cut(s) 494, 919
RsaI GTAC 5 cut(s) 227, 498, 748, 843, 1049
RsaNI GTAC 5 cut(s) 226, 497, 747, 842, 1048
SaqAI TTAA 2 cut(s) 6, 347
SatI GCNGC 9 cut(s) 148, 176, 244, 399, 402, 492, 636, 657, 873
Sau3AI GATC 4 cut(s) 182, 472, 731, 1014
ScaI AGTACT 1 cut(s) 748
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 2 cut(s) 325, 903
SduI GDGCHC 2 cut(s) 47, 686
SfaNI GCATC 1 cut(s) 774
SfcI CTRYAG 3 cut(s) 60, 489, 584
SfuI TTCGAA 2 cut(s) 14, 424
Sse9I AATT 3 cut(s) 420, 426, 786
SspMI CTAG 3 cut(s) 707, 761, 929
StyD4I CCNGG 2 cut(s) 323, 901
TaaI ACNGT 5 cut(s) 411, 465, 517, 802, 1065
TaiI ACGT 2 cut(s) 213, 1031
TaqI TCGA 5 cut(s) 14, 141, 185, 424, 792
TasI AATT 3 cut(s) 420, 426, 786
TatI WGTACW 2 cut(s) 496, 746
TauI GCSGC 5 cut(s) 150, 178, 246, 401, 875
TfiI GAWTC 1 cut(s) 287
Tru1I TTAA 2 cut(s) 6, 347
Tru9I TTAA 2 cut(s) 6, 347
TscAI CASTG 7 cut(s) 299, 543, 651, 729, 775, 858, 940
TseFI GTSAC 1 cut(s) 646
TseI GCWGC 4 cut(s) 401, 491, 635, 656
Tsp45I GTSAC 1 cut(s) 646
TspDTI ATGAA 1 cut(s) 300
TspRI CASTG 7 cut(s) 299, 543, 651, 729, 775, 858, 940
Van91I CCANNNNNTGG 1 cut(s) 728
VneI GTGCAC 1 cut(s) 43
XapI RAATTY 2 cut(s) 420, 426
XceI RCATGY 1 cut(s) 995
XspI CTAG 3 cut(s) 707, 761, 929
ZrmI AGTACT 1 cut(s) 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.