Rw4G031920

CAAX protease self-immunity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004201
Physical Location & Seq
Reverse (-)
59114142 .. 59117084
2943 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G031920.1

Sequence Viewer

Length: 1017 bp
ATGGTTTACACCCTCTTCACATCTCTAGCTCAATCCCCACTTCCCACTTTCTCACACACCCCTTACTTAAATCCCTCCAAAACCTTCCTTAAGCGAACCCACACACTTTTCCTCAATGGTCACACCAGGTCAAACCACCTCTCACGGCTCAGAACCACCAGGCCGCCGCCTCATGTCTGCTTTTCCAGCGGTGGAGGAAAACCCAGACCCGATTTCCAAGAAAAGGAAAATGAAGGGGGCTTGCAAATTTTGAAGAGGTGGGATGTGCCTTGGGAATGGCAAACAGTGTCATTGACATCACTGGCTTGTGGGCTGAGTTTTGTTTTGACAGGATTGGTAGAGGCAGCAGTTGTACCCTATTTAGGATTGAGCATTCAGGATTTGAGTCTAGATGAGAAGGCAGAGTTTCTGTTGGTTGCTCAAGGTGTTACGACTGCAGCCATACTTGGAGTTTTGTATACCGTTGCCGGCAAGTTCCAACCGCTGCCTCAAGATATTTATCGCTATGATTGGAGGGAACCTTTCAGTCTACAGAGAGGGTGGCTTTTGTGGGCAGGGATTGGTCTTGTTGGTGCTGTAGCTGCTGTGGCATTGACAGGGGCTGCCACGTCCTTTTTCAGTGGGCAAACCCCAGAAAGAGAGGTTTGGAACTTGCTCCCATTAATTGGATCATCAAGTATTAGCACTGCTTGCTTGTTAGGCATCACTGGAGTTTTGGCTCCAATTCTTGAGGAAACTGTGTTCCGTGGGTTTTTTATGGTCTCCATGACTAAGTGGGTACCTACACCAGTTGCTGTTGTAATTAGTGCAGCTGTATTTGCTCTTGCACATCTCACTCCTGGAGAGTTTCCCCAGCTGTTTGTGCTAGGCGCTGCTATGGGATTGTCGTATGCTCAAACTCGCAACCTTTTGACCCCCATCACAATACATGCTTTCTGGAACTCGGGAGTTATATTGCTTCTGACCTTCCTTCAGCTTCAAGGGTACGATATCAAGGAATTGTTGCAGGCAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abi

Protein Analysis

338

Amino Acids

36.98

Weight (kDa)

7.77

Isoelectric Point (pI)

41.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rce1-like PF02517 230 - 315 5.6e-21 Type II CAAX prenyl endopeptidase Rce1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 778
AccB1I GGYRCC 1 cut(s) 778
AccB7I CCANNNNNTGG 1 cut(s) 665
AccI GTMKAC 2 cut(s) 458, 529
AciI CCGC 4 cut(s) 164, 167, 189, 482
AclWI GGATC 1 cut(s) 676
AcsI RAATTY 1 cut(s) 246
AcuI CTGAAG 1 cut(s) 956
AfaI GTAC 3 cut(s) 354, 780, 986
AfiI CCNNNNNNNGG 3 cut(s) 223, 362, 665
AflII CTTAAG 1 cut(s) 89
AgsI TTSAA 2 cut(s) 253, 980
AjiI CACGTC 1 cut(s) 609
AjnI CCWGG 3 cut(s) 125, 158, 838
AloI GAACNNNNNNTCC 2 cut(s) 725, 757
AluBI AGCT 5 cut(s) 29, 581, 812, 856, 976
AluI AGCT 5 cut(s) 29, 581, 812, 856, 976
Alw26I GTCTC 1 cut(s) 766
AlwI GGATC 1 cut(s) 676
AlwNI CAGNNNCTG 2 cut(s) 602, 794
Ama87I CYCGRG 1 cut(s) 943
AoxI GGCC 1 cut(s) 161
ApeKI GCWGC 7 cut(s) 344, 437, 484, 581, 602, 809, 872
ApoI RAATTY 1 cut(s) 246
AseI ATTAAT 1 cut(s) 662
Asp718I GGTACC 1 cut(s) 778
AspLEI GCGC 1 cut(s) 872
AvaI CYCGRG 1 cut(s) 943
BanI GGYRCC 1 cut(s) 778
BbvI GCAGC 7 cut(s) 356, 449, 471, 568, 589, 821, 859
BccI CCATC 1 cut(s) 926
BceAI ACGGC 1 cut(s) 161
BciT130I CCWGG 3 cut(s) 127, 160, 840
BcoDI GTCTC 1 cut(s) 766
BfaI CTAG 3 cut(s) 26, 389, 866
BfmI CTRYAG 3 cut(s) 435, 530, 576
BfoI RGCGCY 1 cut(s) 873
BfrI CTTAAG 1 cut(s) 89
BisI GCNGC 9 cut(s) 164, 167, 345, 438, 485, 582, 603, 810, 873
BlsI GCNGC 9 cut(s) 165, 168, 346, 439, 486, 583, 604, 811, 874
Bme1390I CCNGG 3 cut(s) 127, 160, 840
BmeT110I CYCGRG 1 cut(s) 943
BmgBI CACGTC 1 cut(s) 609
BmiI GGNNCC 3 cut(s) 519, 720, 780
BmrFI CCNGG 3 cut(s) 127, 160, 840
BmsI GCATC 1 cut(s) 711
BpmI CTGGAG 2 cut(s) 729, 861
BpuEI CTTGAG 3 cut(s) 405, 474, 749
BsaBI GATNNNNATC 1 cut(s) 498
BsaI GGTCTC 1 cut(s) 766
BsaJI CCNNGG 2 cut(s) 269, 745
Bsc4I CCNNNNNNNGG 3 cut(s) 223, 362, 665
Bse118I RCCGGY 1 cut(s) 467
Bse1I ACTGG 3 cut(s) 306, 712, 788
Bse8I GATNNNNATC 1 cut(s) 498
BseBI CCWGG 3 cut(s) 127, 160, 840
BseDI CCNNGG 2 cut(s) 269, 745
BseGI GGATG 1 cut(s) 268
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 3 cut(s) 223, 362, 665
BseMII CTCAG 2 cut(s) 163, 305
BseNI ACTGG 3 cut(s) 306, 712, 788
BseXI GCAGC 7 cut(s) 356, 449, 471, 568, 589, 821, 859
BseYI CCCAGC 1 cut(s) 852
BsgI GTGCAG 1 cut(s) 828
BshFI GGCC 1 cut(s) 163
BshNI GGYRCC 1 cut(s) 778
BsiHKCI CYCGRG 1 cut(s) 943
BsiSI CCGG 1 cut(s) 468
BslI CCNNNNNNNGG 3 cut(s) 223, 362, 665
BsmAI GTCTC 1 cut(s) 766
BsmI GAATGC 1 cut(s) 372
BsnI GGCC 1 cut(s) 163
Bso31I GGTCTC 1 cut(s) 766
BsoBI CYCGRG 1 cut(s) 943
Bsp143I GATC 1 cut(s) 668
BspACI CCGC 4 cut(s) 164, 167, 189, 482
BspANI GGCC 1 cut(s) 163
BspCNI CTCAG 2 cut(s) 162, 306
BspLI GGNNCC 3 cut(s) 519, 720, 780
BspMAI CTGCAG 1 cut(s) 439
BspPI GGATC 1 cut(s) 676
BspT107I GGYRCC 1 cut(s) 778
BspTI CTTAAG 1 cut(s) 89
BspTNI GGTCTC 1 cut(s) 766
BsrFI RCCGGY 1 cut(s) 467
BsrI ACTGG 3 cut(s) 306, 712, 788
BssAI RCCGGY 1 cut(s) 467
BssECI CCNNGG 2 cut(s) 269, 745
BssMI GATC 1 cut(s) 668
BssNAI GTATAC 1 cut(s) 459
BssT1I CCWWGG 1 cut(s) 269
Bst1107I GTATAC 1 cut(s) 459
Bst2UI CCWGG 3 cut(s) 127, 160, 840
Bst4CI ACNGT 3 cut(s) 286, 463, 739
Bst6I CTCTTC 2 cut(s) 20, 248
BstAFI CTTAAG 1 cut(s) 89
BstAPI GCANNNNNTGC 1 cut(s) 690
BstC8I GCNNGC 4 cut(s) 242, 469, 691, 1008
BstDEI CTNAG 3 cut(s) 149, 314, 771
BstDSI CCRYGG 1 cut(s) 745
BstF5I GGATG 1 cut(s) 268
BstH2I RGCGCY 1 cut(s) 873
BstHHI GCGC 1 cut(s) 872
BstKTI GATC 1 cut(s) 671
BstMAI GTCTC 1 cut(s) 766
BstMBI GATC 1 cut(s) 668
BstMWI GCNNNNNNNGC 7 cut(s) 186, 581, 587, 690, 699, 818, 862
BstNI CCWGG 3 cut(s) 127, 160, 840
BstNSI RCATGY 1 cut(s) 932
BstSCI CCNGG 3 cut(s) 125, 158, 838
BstSFI CTRYAG 3 cut(s) 435, 530, 576
BstV1I GCAGC 7 cut(s) 356, 449, 471, 568, 589, 821, 859
BstZ17I GTATAC 1 cut(s) 459
BsuRI GGCC 1 cut(s) 163
BtgI CCRYGG 1 cut(s) 745
BtrI CACGTC 1 cut(s) 609
BtsCI GGATG 1 cut(s) 268
BtsI GCAGTG 1 cut(s) 684
BtsIMutI CAGTG 5 cut(s) 291, 299, 625, 684, 705
Cac8I GCNNGC 4 cut(s) 242, 469, 691, 1008
CaiI CAGNNNCTG 2 cut(s) 602, 794
CfoI GCGC 1 cut(s) 872
Cfr10I RCCGGY 1 cut(s) 467
CsiI ACCWGGT 1 cut(s) 125
Csp6I GTAC 3 cut(s) 353, 779, 985
CviAII CATG 3 cut(s) 173, 766, 929
CviQI GTAC 3 cut(s) 353, 779, 985
DdeI CTNAG 3 cut(s) 149, 314, 771
DpnI GATC 1 cut(s) 670
DpnII GATC 1 cut(s) 668
Eam1104I CTCTTC 2 cut(s) 20, 248
EarI CTCTTC 2 cut(s) 20, 248
Eco130I CCWWGG 1 cut(s) 269
Eco31I GGTCTC 1 cut(s) 766
Eco32I GATATC 1 cut(s) 991
Eco57I CTGAAG 1 cut(s) 956
Eco88I CYCGRG 1 cut(s) 943
EcoRII CCWGG 3 cut(s) 125, 158, 838
EcoRV GATATC 1 cut(s) 991
EcoT14I CCWWGG 1 cut(s) 269
ErhI CCWWGG 1 cut(s) 269
FaeI CATG 3 cut(s) 176, 769, 932
FatI CATG 3 cut(s) 172, 765, 928
FblI GTMKAC 2 cut(s) 458, 529
Fnu4HI GCNGC 9 cut(s) 164, 167, 345, 438, 485, 582, 603, 810, 873
FokI GGATG 1 cut(s) 275
Fsp4HI GCNGC 9 cut(s) 164, 167, 345, 438, 485, 582, 603, 810, 873
FspBI CTAG 3 cut(s) 26, 389, 866
GlaI GCGC 1 cut(s) 871
GluI GCNGC 9 cut(s) 164, 167, 345, 438, 485, 582, 603, 810, 873
GsaI CCCAGC 1 cut(s) 856
GsuI CTGGAG 2 cut(s) 729, 861
HaeII RGCGCY 1 cut(s) 873
HaeIII GGCC 1 cut(s) 163
HapII CCGG 1 cut(s) 468
HhaI GCGC 1 cut(s) 872
Hin1II CATG 3 cut(s) 176, 769, 932
Hin6I GCGC 1 cut(s) 870
HinP1I GCGC 1 cut(s) 870
HinfI GANTC 1 cut(s) 385
HpaII CCGG 1 cut(s) 468
Hpy166II GTNNAC 3 cut(s) 7, 459, 530
Hpy188I TCNGA 2 cut(s) 152, 963
Hpy188III TCNNGA 6 cut(s) 377, 389, 491, 728, 937, 945
Hpy8I GTNNAC 3 cut(s) 7, 459, 530
HpyAV CCTTC 5 cut(s) 94, 227, 391, 976, 980
HpyCH4III ACNGT 3 cut(s) 286, 463, 739
HpyCH4IV ACGT 1 cut(s) 608
HpyCH4V TGCA 5 cut(s) 244, 437, 809, 827, 1006
HpyF10VI GCNNNNNNNGC 7 cut(s) 186, 581, 587, 690, 699, 818, 862
HpyF3I CTNAG 3 cut(s) 149, 314, 771
HpySE526I ACGT 1 cut(s) 608
Hsp92II CATG 3 cut(s) 176, 769, 932
HspAI GCGC 1 cut(s) 870
KpnI GGTACC 1 cut(s) 782
KroI GCCGGC 1 cut(s) 467
KroNI GCCGGC 1 cut(s) 469
Kzo9I GATC 1 cut(s) 668
LmnI GCTCC 2 cut(s) 660, 724
Lsp1109I GCAGC 7 cut(s) 356, 449, 471, 568, 589, 821, 859
LweI GCATC 1 cut(s) 711
MabI ACCWGGT 1 cut(s) 125
MaeI CTAG 3 cut(s) 26, 389, 866
MaeII ACGT 1 cut(s) 608
MaeIII GTNAC 2 cut(s) 119, 427
MalI GATC 1 cut(s) 670
MboI GATC 1 cut(s) 668
MboII GAAGA 2 cut(s) 7, 265
MluCI AATT 5 cut(s) 246, 663, 723, 801, 998
MlyI GAGTC 1 cut(s) 394
MmeI TCCRAC 1 cut(s) 502
MroNI GCCGGC 1 cut(s) 467
MseI TTAA 3 cut(s) 68, 90, 662
MspA1I CMGCKG 4 cut(s) 189, 484, 812, 856
MspCI CTTAAG 1 cut(s) 89
MspI CCGG 1 cut(s) 468
MspR9I CCNGG 3 cut(s) 127, 160, 840
Mva1269I GAATGC 1 cut(s) 372
MvaI CCWGG 3 cut(s) 127, 160, 840
MwoI GCNNNNNNNGC 7 cut(s) 186, 581, 587, 690, 699, 818, 862
NaeI GCCGGC 1 cut(s) 469
NdeII GATC 1 cut(s) 668
NgoMIV GCCGGC 1 cut(s) 467
NlaIII CATG 3 cut(s) 176, 769, 932
NlaIV GGNNCC 3 cut(s) 519, 720, 780
NmuCI GTSAC 1 cut(s) 119
NspI RCATGY 1 cut(s) 932
PctI GAATGC 1 cut(s) 372
PdiI GCCGGC 1 cut(s) 469
PflMI CCANNNNNTGG 1 cut(s) 665
PfoI TCCNGGA 1 cut(s) 838
PkrI GCNGC 9 cut(s) 165, 168, 346, 439, 486, 583, 604, 811, 874
PleI GAGTC 1 cut(s) 393
PpsI GAGTC 1 cut(s) 393
PshBI ATTAAT 1 cut(s) 662
Psp6I CCWGG 3 cut(s) 125, 158, 838
PspFI CCCAGC 1 cut(s) 852
PspGI CCWGG 3 cut(s) 125, 158, 838
PspN4I GGNNCC 3 cut(s) 519, 720, 780
PstI CTGCAG 1 cut(s) 439
PstNI CAGNNNCTG 2 cut(s) 602, 794
PvuII CAGCTG 2 cut(s) 812, 856
RsaI GTAC 3 cut(s) 354, 780, 986
RsaNI GTAC 3 cut(s) 353, 779, 985
SaqAI TTAA 3 cut(s) 68, 90, 662
SatI GCNGC 9 cut(s) 164, 167, 345, 438, 485, 582, 603, 810, 873
Sau3AI GATC 1 cut(s) 668
SchI GAGTC 1 cut(s) 394
ScrFI CCNGG 3 cut(s) 127, 160, 840
SexAI ACCWGGT 1 cut(s) 125
SfaNI GCATC 1 cut(s) 711
SfcI CTRYAG 3 cut(s) 435, 530, 576
SmlI CTYRAG 4 cut(s) 89, 420, 489, 728
SmoI CTYRAG 4 cut(s) 89, 420, 489, 728
Sse9I AATT 5 cut(s) 246, 663, 723, 801, 998
SsiI CCGC 4 cut(s) 164, 167, 189, 482
SspMI CTAG 3 cut(s) 26, 389, 866
StyD4I CCNGG 3 cut(s) 125, 158, 838
StyI CCWWGG 1 cut(s) 269
TaaI ACNGT 3 cut(s) 286, 463, 739
TaiI ACGT 1 cut(s) 611
TasI AATT 5 cut(s) 246, 663, 723, 801, 998
TauI GCSGC 2 cut(s) 166, 169
Tru1I TTAA 3 cut(s) 68, 90, 662
Tru9I TTAA 3 cut(s) 68, 90, 662
TscAI CASTG 5 cut(s) 291, 306, 625, 691, 712
TseFI GTSAC 1 cut(s) 119
TseI GCWGC 7 cut(s) 344, 437, 484, 581, 602, 809, 872
Tsp45I GTSAC 1 cut(s) 119
TspDTI ATGAA 1 cut(s) 246
TspGWI ACGGA 1 cut(s) 734
TspRI CASTG 5 cut(s) 291, 306, 625, 691, 712
Van91I CCANNNNNTGG 1 cut(s) 665
Vha464I CTTAAG 1 cut(s) 89
VspI ATTAAT 1 cut(s) 662
XapI RAATTY 1 cut(s) 246
XbaI TCTAGA 1 cut(s) 388
XceI RCATGY 1 cut(s) 932
XmiI GTMKAC 2 cut(s) 458, 529
XspI CTAG 3 cut(s) 26, 389, 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.